pycom12g18210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014347
Physical Location & Seq
Forward (+)
20409795 .. 20410282
488 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18210.1

Sequence Viewer

Length: 393 bp
ATGGACGACGCCACGAACAGTAACACCATAAAGTTGGTGGTTTTCTTAATCTTCATCATGGGGCTGCTATTAACCGTTAACCCTGCATCAGCAACTGTAGAGACGTATGAGCTAAACCTAAACCAATCAACAACAACGGAAACATTTGATCCCGCCTGTTCGACGGAGTATAAGCTATTGATCGATGGCGTGACTAAAACTCATCACGATACACAAAGGCGGCGGCTAGCGCCGTATCAGCTATGCTTGCAGTGTAAGTGCTGCTCAGCCACCACCTGCATCTTCATGCCTTGCTGCTTTGGCATCAACTGCCAACTCCCCAACAAACCCTTTGGAGTTTGCGGTTTTGTCCCCAAGACTTGCAACTGCACCAATTGCACAACTCCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

14.3

Weight (kDa)

6.66

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7866 PF25268 78 - 127 1.4e-19 Domain of unknown function (DUF7866)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014347)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01015 AT5G01015
fragaria_vesca FvH4_6g09130
malus_domestica MD04G1180500.v1.1 MD12G1195900.v1.1
prunus_persica Prupe.6G300400_v2.0.a1
pyrus_communis pycom04g15960 pycom12g18210
rosa_chinensis RchiOBHm_Chr3g0459341
rosa_laevigata RLG00000025089
rosa_roxburghii Rroxscaffold_6G00420250
rosa_rugosa Rorug03G0035500
rosa_samantha Rh3AG093600 Rh3BG096500 Rh3CG097700 Rh3DG097100
rosa_wichuraiana Rw3G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 284
AciI CCGC 4 cut(s) 153, 220, 223, 342
AclWI GGATC 1 cut(s) 143
AcyI GRCGYC 1 cut(s) 9
AluBI AGCT 3 cut(s) 112, 175, 241
AluI AGCT 3 cut(s) 112, 175, 241
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 1 cut(s) 143
AlwNI CAGNNNCTG 1 cut(s) 95
ApeKI GCWGC 3 cut(s) 64, 261, 294
AspLEI GCGC 1 cut(s) 232
AsuNHI GCTAGC 1 cut(s) 226
BbvI GCAGC 3 cut(s) 51, 248, 281
BccI CCATC 1 cut(s) 179
BceAI ACGGC 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 1 cut(s) 227
BfmI CTRYAG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 284
BisI GCNGC 5 cut(s) 65, 221, 224, 262, 295
BlpI GCTNAGC 1 cut(s) 265
BlsI GCNGC 5 cut(s) 66, 222, 225, 263, 296
BmsI GCATC 3 cut(s) 95, 288, 312
BmtI GCTAGC 1 cut(s) 230
Bpu1102I GCTNAGC 1 cut(s) 265
Bsa29I ATCGAT 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 9
BseCI ATCGAT 1 cut(s) 183
BseMII CTCAG 1 cut(s) 279
BseXI GCAGC 3 cut(s) 51, 248, 281
BsgI GTGCAG 1 cut(s) 352
BshVI ATCGAT 1 cut(s) 183
BslFI GGGAC 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 95
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 335
Bsp143I GATC 2 cut(s) 148, 180
Bsp1720I GCTNAGC 1 cut(s) 265
BspACI CCGC 4 cut(s) 153, 220, 223, 342
BspCNI CTCAG 1 cut(s) 278
BspDI ATCGAT 1 cut(s) 183
BspMI ACCTGC 1 cut(s) 284
BspOI GCTAGC 1 cut(s) 230
BspPI GGATC 1 cut(s) 143
BssMI GATC 2 cut(s) 148, 180
BssNI GRCGYC 1 cut(s) 9
Bst4CI ACNGT 3 cut(s) 20, 76, 97
BstACI GRCGYC 1 cut(s) 9
BstAPI GCANNNNNTGC 2 cut(s) 309, 375
BstC8I GCNNGC 2 cut(s) 228, 248
BstDEI CTNAG 1 cut(s) 265
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 2 cut(s) 151, 183
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 2 cut(s) 148, 180
BstMWI GCNNNNNNNGC 6 cut(s) 229, 238, 247, 300, 309, 375
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 51, 248, 281
BstXI CCANNNNNNTGG 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 183
BsuTUI ATCGAT 1 cut(s) 183
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 1 cut(s) 257
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 2 cut(s) 228, 248
CaiI CAGNNNCTG 1 cut(s) 95
CfoI GCGC 1 cut(s) 232
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 1 cut(s) 17
CviAII CATG 2 cut(s) 58, 286
CviJI RGCY 6 cut(s) 64, 112, 175, 226, 241, 269
CviKI_1 RGCY 6 cut(s) 64, 112, 175, 226, 241, 269
DdeI CTNAG 1 cut(s) 265
DpnI GATC 2 cut(s) 150, 182
DpnII GATC 2 cut(s) 148, 180
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 2 cut(s) 61, 289
FaiI YATR 6 cut(s) 29, 59, 108, 171, 244, 287
FaqI GGGAC 1 cut(s) 335
FatI CATG 2 cut(s) 57, 285
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 5 cut(s) 65, 221, 224, 262, 295
Fsp4HI GCNGC 5 cut(s) 65, 221, 224, 262, 295
FspBI CTAG 1 cut(s) 227
GlaI GCGC 1 cut(s) 231
GluI GCNGC 5 cut(s) 65, 221, 224, 262, 295
HaeII RGCGCY 1 cut(s) 233
HgaI GACGC 1 cut(s) 17
HhaI GCGC 1 cut(s) 232
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 2 cut(s) 61, 289
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HpaI GTTAAC 1 cut(s) 79
Hpy166II GTNNAC 1 cut(s) 79
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 11, 166
HpyCH4III ACNGT 3 cut(s) 20, 76, 97
HpyCH4IV ACGT 1 cut(s) 104
HpyCH4V TGCA 6 cut(s) 86, 250, 279, 363, 369, 378
HpyF10VI GCNNNNNNNGC 6 cut(s) 229, 238, 247, 300, 309, 375
HpyF3I CTNAG 1 cut(s) 265
HpySE526I ACGT 1 cut(s) 104
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 2 cut(s) 61, 289
HspAI GCGC 1 cut(s) 230
KspAI GTTAAC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 148, 180
LpnPI CCDG 3 cut(s) 96, 169, 289
Lsp1109I GCAGC 3 cut(s) 51, 248, 281
LweI GCATC 3 cut(s) 95, 288, 312
MaeI CTAG 1 cut(s) 227
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 2 cut(s) 20, 190
MalI GATC 2 cut(s) 150, 182
MboI GATC 2 cut(s) 148, 180
MboII GAAGA 2 cut(s) 43, 274
MfeI CAATTG 1 cut(s) 373
MluCI AATT 1 cut(s) 373
MseI TTAA 3 cut(s) 47, 71, 78
MslI CAYNNNNRTG 1 cut(s) 284
MunI CAATTG 1 cut(s) 373
MwoI GCNNNNNNNGC 6 cut(s) 229, 238, 247, 300, 309, 375
NdeII GATC 2 cut(s) 148, 180
NheI GCTAGC 1 cut(s) 226
NlaIII CATG 2 cut(s) 61, 289
NmuCI GTSAC 1 cut(s) 190
PaqCI CACCTGC 1 cut(s) 284
PkrI GCNGC 5 cut(s) 66, 222, 225, 263, 296
PstNI CAGNNNCTG 1 cut(s) 95
RseI CAYNNNNRTG 1 cut(s) 284
SaqAI TTAA 3 cut(s) 47, 71, 78
SatI GCNGC 5 cut(s) 65, 221, 224, 262, 295
Sau3AI GATC 2 cut(s) 148, 180
SetI ASST 6 cut(s) 107, 114, 120, 177, 243, 278
SfaNI GCATC 3 cut(s) 95, 288, 312
SfcI CTRYAG 1 cut(s) 96
SmiMI CAYNNNNRTG 1 cut(s) 284
Sse9I AATT 1 cut(s) 373
SsiI CCGC 4 cut(s) 153, 220, 223, 342
SspMI CTAG 1 cut(s) 227
TaaI ACNGT 3 cut(s) 20, 76, 97
TaiI ACGT 1 cut(s) 107
TaqI TCGA 2 cut(s) 161, 183
TasI AATT 1 cut(s) 373
TauI GCSGC 2 cut(s) 223, 226
Tru1I TTAA 3 cut(s) 47, 71, 78
Tru9I TTAA 3 cut(s) 47, 71, 78
TscAI CASTG 1 cut(s) 257
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 3 cut(s) 64, 261, 294
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 2 cut(s) 43, 274
TspGWI ACGGA 2 cut(s) 152, 179
TspRI CASTG 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XspI CTAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.