Rroxscaffold_6G00420250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014347
Physical Location & Seq
Forward (+)
41562424 .. 41563375
952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00420250.1

Sequence Viewer

Length: 378 bp
ATGAATGACTACGATTTCATAATCCGCAGCACCATCAAATCAGTTGTAGCCATAGTCCTCTTCATGGCACTATTGGCCATTAGTAGTACTACTACTACTGCAGTATCTCATGATGTTCTTGAGTTTATTCCAATGAAGAATTCAATGGAGTTTCAAGTATTGGACGTTGTTAGAGCTCACGATGAAGCACGAAGACGGAGGCTGGAGCCCTACCAACTATGCTTGGTTTGCAAGTGCTGCGCCGGCACAACATGCTCCACCATGCCTTGCTGTTTCGGCATAGACTGCCAGCTTCCCGGGAAGCCCTTTGGGGTTTGCGCCTTTGTTCCAAAGATCTGCAACTGTACCAACTGTACAAGTAGTAGTAGTACTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.73

Weight (kDa)

7.44

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7866 PF25268 69 - 119 1.3e-19 Domain of unknown function (DUF7866)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014347)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01015 AT5G01015
fragaria_vesca FvH4_6g09130
malus_domestica MD04G1180500.v1.1 MD12G1195900.v1.1
prunus_persica Prupe.6G300400_v2.0.a1
pyrus_communis pycom04g15960 pycom12g18210
rosa_chinensis RchiOBHm_Chr3g0459341
rosa_laevigata RLG00000025089
rosa_roxburghii Rroxscaffold_6G00420250
rosa_rugosa Rorug03G0035500
rosa_samantha Rh3AG093600 Rh3BG096500 Rh3CG097700 Rh3DG097100
rosa_wichuraiana Rw3G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 25
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 1 cut(s) 139
AfaI GTAC 4 cut(s) 88, 346, 355, 370
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 2 cut(s) 144, 155
AjuI GAANNNNNNNTTGG 2 cut(s) 124, 156
AluBI AGCT 2 cut(s) 176, 292
AluI AGCT 2 cut(s) 176, 292
Alw21I GWGCWC 1 cut(s) 178
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 2 cut(s) 27, 237
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 2 cut(s) 242, 320
AsuC2I CCSGG 2 cut(s) 297, 298
AvaI CYCGRG 1 cut(s) 296
BaeI ACNNNNGTAYC 2 cut(s) 87, 120
BalI TGGCCA 1 cut(s) 77
BanII GRGCYC 2 cut(s) 178, 210
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 39, 224
BccI CCATC 1 cut(s) 41
BcnI CCSGG 2 cut(s) 297, 298
BfmI CTRYAG 1 cut(s) 99
BglII AGATCT 1 cut(s) 333
BisI GCNGC 2 cut(s) 28, 238
BlsI GCNGC 2 cut(s) 29, 239
BmcAI AGTACT 2 cut(s) 88, 370
Bme1390I CCNGG 2 cut(s) 297, 298
BmeT110I CYCGRG 1 cut(s) 296
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 2 cut(s) 297, 298
BpiI GAAGAC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 2 cut(s) 297, 298
BsaJI CCNNGG 1 cut(s) 296
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse118I RCCGGY 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 296
BseLI CCNNNNNNNGG 1 cut(s) 64
BseXI GCAGC 2 cut(s) 39, 224
BshFI GGCC 1 cut(s) 77
BsiHKAI GWGCWC 1 cut(s) 178
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 2 cut(s) 243, 297
BslI CCNNNNNNNGG 1 cut(s) 64
BsnI GGCC 1 cut(s) 77
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 178, 210
Bsp1407I TGTACA 1 cut(s) 353
Bsp143I GATC 1 cut(s) 333
BspACI CCGC 1 cut(s) 25
BspANI GGCC 1 cut(s) 77
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 353
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 296
BssMI GATC 1 cut(s) 333
Bst4CI ACNGT 2 cut(s) 344, 353
Bst6I CTCTTC 1 cut(s) 65
BstAPI GCANNNNNTGC 3 cut(s) 237, 252, 285
BstAUI TGTACA 1 cut(s) 353
BstC8I GCNNGC 2 cut(s) 244, 290
BstHHI GCGC 2 cut(s) 242, 320
BstKTI GATC 1 cut(s) 336
BstMBI GATC 1 cut(s) 333
BstMWI GCNNNNNNNGC 7 cut(s) 74, 228, 237, 243, 252, 276, 285
BstNSI RCATGY 1 cut(s) 255
BstSCI CCNGG 2 cut(s) 295, 296
BstSFI CTRYAG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 39, 224
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 1 cut(s) 77
Cac8I GCNNGC 2 cut(s) 244, 290
CciI TCATGA 1 cut(s) 109
CfoI GCGC 2 cut(s) 242, 320
Cfr10I RCCGGY 1 cut(s) 242
Cfr9I CCCGGG 1 cut(s) 296
Csp6I GTAC 4 cut(s) 87, 345, 354, 369
CviAII CATG 4 cut(s) 64, 110, 252, 262
CviJI RGCY 7 cut(s) 50, 77, 176, 202, 208, 292, 304
CviKI_1 RGCY 7 cut(s) 50, 77, 176, 202, 208, 292, 304
CviQI GTAC 4 cut(s) 87, 345, 354, 369
DpnI GATC 1 cut(s) 335
DpnII GATC 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 176
Eco24I GRGCYC 2 cut(s) 178, 210
Eco53kI GAGCTC 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 139
EcoT38I GRGCYC 2 cut(s) 178, 210
FaeI CATG 4 cut(s) 67, 113, 255, 265
FaiI YATR 8 cut(s) 20, 53, 65, 111, 220, 253, 263, 281
FatI CATG 4 cut(s) 63, 109, 251, 261
Fnu4HI GCNGC 2 cut(s) 28, 238
FriOI GRGCYC 2 cut(s) 178, 210
Fsp4HI GCNGC 2 cut(s) 28, 238
GlaI GCGC 2 cut(s) 241, 319
GluI GCNGC 2 cut(s) 28, 238
GsuI CTGGAG 1 cut(s) 224
HaeIII GGCC 1 cut(s) 77
HapII CCGG 2 cut(s) 243, 297
HhaI GCGC 2 cut(s) 242, 320
Hin1II CATG 4 cut(s) 67, 113, 255, 265
Hin6I GCGC 2 cut(s) 240, 318
HinP1I GCGC 2 cut(s) 240, 318
HpaII CCGG 2 cut(s) 243, 297
Hpy188III TCNNGA 3 cut(s) 110, 119, 179
HpyCH4III ACNGT 2 cut(s) 344, 353
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 3 cut(s) 101, 231, 339
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 228, 237, 243, 252, 276, 285
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 4 cut(s) 67, 113, 255, 265
HspAI GCGC 2 cut(s) 240, 318
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
Kzo9I GATC 1 cut(s) 333
LmnI GCTCC 2 cut(s) 205, 260
LpnPI CCDG 4 cut(s) 188, 256, 302, 310
Lsp1109I GCAGC 2 cut(s) 39, 224
MaeII ACGT 1 cut(s) 165
MalI GATC 1 cut(s) 335
MboI GATC 1 cut(s) 333
MboII GAAGA 3 cut(s) 52, 148, 204
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 2 cut(s) 178, 210
MlsI TGGCCA 1 cut(s) 77
MluCI AATT 1 cut(s) 139
MluNI TGGCCA 1 cut(s) 77
MnlI CCTC 2 cut(s) 68, 192
Mox20I TGGCCA 1 cut(s) 77
MroNI GCCGGC 1 cut(s) 242
MscI TGGCCA 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 77
MspI CCGG 2 cut(s) 243, 297
MspR9I CCNGG 2 cut(s) 297, 298
MwoI GCNNNNNNNGC 7 cut(s) 74, 228, 237, 243, 252, 276, 285
NaeI GCCGGC 1 cut(s) 244
NciI CCSGG 2 cut(s) 297, 298
NdeII GATC 1 cut(s) 333
NgoMIV GCCGGC 1 cut(s) 242
NlaIII CATG 4 cut(s) 67, 113, 255, 265
NlaIV GGNNCC 1 cut(s) 207
NspI RCATGY 1 cut(s) 255
PagI TCATGA 1 cut(s) 109
PdiI GCCGGC 1 cut(s) 244
PkrI GCNGC 2 cut(s) 29, 239
Psp124BI GAGCTC 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 207
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 4 cut(s) 88, 346, 355, 370
RsaNI GTAC 4 cut(s) 87, 345, 354, 369
SacI GAGCTC 1 cut(s) 178
SatI GCNGC 2 cut(s) 28, 238
Sau3AI GATC 1 cut(s) 333
ScaI AGTACT 2 cut(s) 88, 370
ScrFI CCNGG 2 cut(s) 297, 298
SduI GDGCHC 2 cut(s) 178, 210
SetI ASST 3 cut(s) 168, 178, 294
SfcI CTRYAG 1 cut(s) 99
SmaI CCCGGG 1 cut(s) 298
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 1 cut(s) 139
SsiI CCGC 1 cut(s) 25
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 2 cut(s) 295, 296
TaaI ACNGT 2 cut(s) 344, 353
TaiI ACGT 1 cut(s) 168
TasI AATT 1 cut(s) 139
TatI WGTACW 3 cut(s) 86, 353, 368
TseI GCWGC 2 cut(s) 27, 237
TspDTI ATGAA 5 cut(s) 7, 17, 52, 149, 198
TspGWI ACGGA 1 cut(s) 211
TspMI CCCGGG 1 cut(s) 296
XapI RAATTY 1 cut(s) 139
XceI RCATGY 1 cut(s) 255
XmaI CCCGGG 1 cut(s) 296
ZrmI AGTACT 2 cut(s) 88, 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.