MD12G1195900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014347
Physical Location & Seq
Forward (+)
27695028 .. 27695604
577 bp
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UTR
Exon/CDS
Intron
MD12G1195900.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGACGACGCCACAAACAGTAACACCATAAAGTTGGTGGTTTTCTTAATCTTCATCATGGGGCTGCTATTAACCGTTAACCCTGCATCAGCAACTGAGACGTACGAGCCAAACCTAAACCAATCAACAACAACGGAAACATTTCATCCCGCCTGTTCGACGGAGTATAAGCTATTGATCGATGGCGTGACTAAAACTCATCACGATACACAAAGGCGGCGGCTAGCGCCGTATCAGCTATGCTTGCAGTGCAAGTGCTGCTCAGCCATCACCTGCATCTCCATGCCTTGCTGCTTCGGCATCAACTGCCAACTCCCCAACAAACCCTTTGGAGTTTGCGGTTTTGTCCCCAAGACTTGCAACTGCACCAATTGCACAACTCCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.16

Weight (kDa)

7.44

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7866 PF25268 77 - 126 1.1e-19 Domain of unknown function (DUF7866)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014347)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01015 AT5G01015
fragaria_vesca FvH4_6g09130
malus_domestica MD04G1180500.v1.1 MD12G1195900.v1.1
prunus_persica Prupe.6G300400_v2.0.a1
pyrus_communis pycom04g15960 pycom12g18210
rosa_chinensis RchiOBHm_Chr3g0459341
rosa_laevigata RLG00000025089
rosa_roxburghii Rroxscaffold_6G00420250
rosa_rugosa Rorug03G0035500
rosa_samantha Rh3AG093600 Rh3BG096500 Rh3CG097700 Rh3DG097100
rosa_wichuraiana Rw3G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 281
AciI CCGC 4 cut(s) 150, 217, 220, 339
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 1 cut(s) 104
AluBI AGCT 2 cut(s) 172, 238
AluI AGCT 2 cut(s) 172, 238
Alw26I GTCTC 1 cut(s) 92
AlwNI CAGNNNCTG 1 cut(s) 95
ApeKI GCWGC 3 cut(s) 64, 258, 291
Asp700I GAANNNNTTC 1 cut(s) 141
AspLEI GCGC 1 cut(s) 229
AsuHPI GGTGA 1 cut(s) 262
AsuNHI GCTAGC 1 cut(s) 223
BbvI GCAGC 3 cut(s) 51, 245, 278
BccI CCATC 2 cut(s) 176, 275
BceAI ACGGC 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 224
BfoI RGCGCY 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 281
BisI GCNGC 5 cut(s) 65, 218, 221, 259, 292
BlpI GCTNAGC 1 cut(s) 262
BlsI GCNGC 5 cut(s) 66, 219, 222, 260, 293
BmsI GCATC 3 cut(s) 95, 285, 309
BmtI GCTAGC 1 cut(s) 227
Bpu1102I GCTNAGC 1 cut(s) 262
Bsa29I ATCGAT 1 cut(s) 180
BsaHI GRCGYC 1 cut(s) 9
BseCI ATCGAT 1 cut(s) 180
BseGI GGATG 1 cut(s) 145
BseMII CTCAG 2 cut(s) 87, 276
BseXI GCAGC 3 cut(s) 51, 245, 278
BsgI GTGCAG 1 cut(s) 349
BshVI ATCGAT 1 cut(s) 180
BsiWI CGTACG 1 cut(s) 102
BslFI GGGAC 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 92
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 332
Bsp143I GATC 1 cut(s) 177
Bsp1720I GCTNAGC 1 cut(s) 262
BspACI CCGC 4 cut(s) 150, 217, 220, 339
BspCNI CTCAG 2 cut(s) 88, 275
BspDI ATCGAT 1 cut(s) 180
BspMI ACCTGC 1 cut(s) 281
BspOI GCTAGC 1 cut(s) 227
BssMI GATC 1 cut(s) 177
BssNI GRCGYC 1 cut(s) 9
Bst4CI ACNGT 2 cut(s) 20, 76
BstACI GRCGYC 1 cut(s) 9
BstAPI GCANNNNNTGC 3 cut(s) 258, 306, 372
BstC8I GCNNGC 2 cut(s) 225, 245
BstDEI CTNAG 2 cut(s) 96, 262
BstF5I GGATG 1 cut(s) 145
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 1 cut(s) 180
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 1 cut(s) 177
BstMWI GCNNNNNNNGC 8 cut(s) 226, 235, 244, 249, 258, 297, 306, 372
BstV1I GCAGC 3 cut(s) 51, 245, 278
BstXI CCANNNNNNTGG 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 180
BsuTUI ATCGAT 1 cut(s) 180
BtsCI GGATG 1 cut(s) 145
BtsI GCAGTG 1 cut(s) 254
BtsIMutI CAGTG 1 cut(s) 254
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 2 cut(s) 225, 245
CaiI CAGNNNCTG 1 cut(s) 95
CfoI GCGC 1 cut(s) 229
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 103
CviAII CATG 2 cut(s) 58, 283
CviJI RGCY 6 cut(s) 64, 109, 172, 223, 238, 266
CviKI_1 RGCY 6 cut(s) 64, 109, 172, 223, 238, 266
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 2 cut(s) 96, 262
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 2 cut(s) 61, 286
FaiI YATR 5 cut(s) 29, 59, 168, 241, 284
FaqI GGGAC 1 cut(s) 332
FatI CATG 2 cut(s) 57, 282
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 5 cut(s) 65, 218, 221, 259, 292
FokI GGATG 1 cut(s) 132
Fsp4HI GCNGC 5 cut(s) 65, 218, 221, 259, 292
FspBI CTAG 1 cut(s) 224
GlaI GCGC 1 cut(s) 228
GluI GCNGC 5 cut(s) 65, 218, 221, 259, 292
HaeII RGCGCY 1 cut(s) 230
HgaI GACGC 1 cut(s) 17
HhaI GCGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 2 cut(s) 61, 286
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HpaI GTTAAC 1 cut(s) 79
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 79
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 11, 163
HpyCH4III ACNGT 2 cut(s) 20, 76
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 7 cut(s) 86, 247, 252, 276, 360, 366, 375
HpyF10VI GCNNNNNNNGC 8 cut(s) 226, 235, 244, 249, 258, 297, 306, 372
HpyF3I CTNAG 2 cut(s) 96, 262
HpySE526I ACGT 1 cut(s) 101
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 2 cut(s) 61, 286
HspAI GCGC 1 cut(s) 227
KspAI GTTAAC 1 cut(s) 79
Kzo9I GATC 1 cut(s) 177
LpnPI CCDG 3 cut(s) 96, 166, 286
Lsp1109I GCAGC 3 cut(s) 51, 245, 278
LweI GCATC 3 cut(s) 95, 285, 309
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 2 cut(s) 20, 187
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MboII GAAGA 1 cut(s) 43
MfeI CAATTG 1 cut(s) 370
MluCI AATT 1 cut(s) 370
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 3 cut(s) 47, 71, 78
MslI CAYNNNNRTG 1 cut(s) 281
MunI CAATTG 1 cut(s) 370
MwoI GCNNNNNNNGC 8 cut(s) 226, 235, 244, 249, 258, 297, 306, 372
NdeII GATC 1 cut(s) 177
NheI GCTAGC 1 cut(s) 223
NlaIII CATG 2 cut(s) 61, 286
NmuCI GTSAC 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 281
PdmI GAANNNNTTC 1 cut(s) 141
Pfl23II CGTACG 1 cut(s) 102
PkrI GCNGC 5 cut(s) 66, 219, 222, 260, 293
PspLI CGTACG 1 cut(s) 102
PstNI CAGNNNCTG 1 cut(s) 95
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 3 cut(s) 47, 71, 78
SatI GCNGC 5 cut(s) 65, 218, 221, 259, 292
Sau3AI GATC 1 cut(s) 177
SetI ASST 5 cut(s) 104, 117, 174, 240, 275
SfaNI GCATC 3 cut(s) 95, 285, 309
SmiMI CAYNNNNRTG 1 cut(s) 281
Sse9I AATT 1 cut(s) 370
SsiI CCGC 4 cut(s) 150, 217, 220, 339
SspMI CTAG 1 cut(s) 224
TaaI ACNGT 2 cut(s) 20, 76
TaiI ACGT 1 cut(s) 104
TaqI TCGA 2 cut(s) 158, 180
TasI AATT 1 cut(s) 370
TauI GCSGC 2 cut(s) 220, 223
Tru1I TTAA 3 cut(s) 47, 71, 78
Tru9I TTAA 3 cut(s) 47, 71, 78
TscAI CASTG 1 cut(s) 254
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 3 cut(s) 64, 258, 291
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 43, 134
TspGWI ACGGA 2 cut(s) 149, 176
TspRI CASTG 1 cut(s) 254
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.