Rorug03G0035500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014347
Physical Location & Seq
Forward (+)
2819440 .. 2825219
5780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0035500.1

Sequence Viewer

Length: 672 bp
ATGGCGGGAGTGTGCTGCTCTCTGAGACTACAGATTCCTCTGCTCTCACACCCCACGAGAAACCATGGAATCCGGTGCTGCGCAAAAGCACCAGATAACAATCCCAAGAGAGAAAAAACTTCGCAGATATTGAAACTCGCAGTGTCCGGAGTCACTGAGTTCCTCAGGCTTCTATCTTCGTTCAACACAACAACAACAACCAGATTGAATAAAGCGACTGATAAACAAAGGGATGAGTTTTCGAATGTGGGAATAGATGATGTTTTGATGATTCTCGAAGCGGATTACAAGAATGCGTATTTTGTGACAGGCATTCTCACCTCTGCAATCTATGCTGAAGACTGTATCTTTGAAGATCCAACTATTAGGTTTCAAGGTACGGAGTTGTATGCTCGCAACTTGAAATTGCTTGTTCCTTTCTTTGACTCTCCATCAATTGGATTGGAAAAGATTGAGAAGGGTATTGATTCTGAAGCGAACTTTGTTCTAGCAACATGGAAACTAAGGACCTACCTGAAGCTCCCATGGAGGCCTTTGATTTCTATTGATGGAAGTACAGTCTATGACTTGGATGATAAGTTTAAAATTGTAAGGCATGCTGAGAGTTGGAATGTTTCTGCAATTGAGGCAGTTGGCCAGATATTCAGACCTTCTGGAAGGTCCGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

25.11

Weight (kDa)

6.83

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 87 - 199 1e-22 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014347)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01015 AT5G01015
fragaria_vesca FvH4_6g09130
malus_domestica MD04G1180500.v1.1 MD12G1195900.v1.1
prunus_persica Prupe.6G300400_v2.0.a1
pyrus_communis pycom04g15960 pycom12g18210
rosa_chinensis RchiOBHm_Chr3g0459341
rosa_laevigata RLG00000025089
rosa_roxburghii Rroxscaffold_6G00420250
rosa_rugosa Rorug03G0035500
rosa_samantha Rh3AG093600 Rh3BG096500 Rh3CG097700 Rh3DG097100
rosa_wichuraiana Rw3G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 437
AccIII TCCGGA 1 cut(s) 146
AciI CCGC 2 cut(s) 5, 281
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 1 cut(s) 634
AcuI CTGAAG 3 cut(s) 357, 492, 536
AfaI GTAC 2 cut(s) 379, 556
AfiI CCNNNNNNNGG 1 cut(s) 437
AgsI TTSAA 6 cut(s) 133, 184, 208, 353, 374, 403
AluBI AGCT 1 cut(s) 520
AluI AGCT 1 cut(s) 520
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 350
Aor13HI TCCGGA 1 cut(s) 146
AoxI GGCC 2 cut(s) 530, 634
ApeKI GCWGC 2 cut(s) 15, 78
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 507, 660
AsuHPI GGTGA 1 cut(s) 310
AsuII TTCGAA 1 cut(s) 242
AvaII GGWCC 2 cut(s) 507, 660
AxyI CCTNAGG 1 cut(s) 164
BalI TGGCCA 1 cut(s) 636
BauI CACGAG 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 2, 65
BccI CCATC 2 cut(s) 439, 542
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 1 cut(s) 488
BfmI CTRYAG 1 cut(s) 29
BisI GCNGC 2 cut(s) 16, 79
BlsI GCNGC 2 cut(s) 17, 80
Bme18I GGWCC 2 cut(s) 507, 660
BmgT120I GGNCC 2 cut(s) 507, 660
BpiI GAAGAC 1 cut(s) 345
Bpu14I TTCGAA 1 cut(s) 242
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 64, 524
BsaWI WCCGGW 2 cut(s) 72, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse21I CCTNAGG 1 cut(s) 164
Bse8I GATNNNNATC 1 cut(s) 99
BseAI TCCGGA 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 64, 524
BseGI GGATG 2 cut(s) 238, 577
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 437
BseMII CTCAG 4 cut(s) 14, 147, 178, 591
BseXI GCAGC 2 cut(s) 2, 65
BshFI GGCC 2 cut(s) 532, 636
BsiSI CCGG 2 cut(s) 73, 147
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 1 cut(s) 19
BsmI GAATGC 2 cut(s) 298, 312
BsnI GGCC 2 cut(s) 532, 636
Bsp119I TTCGAA 1 cut(s) 242
Bsp13I TCCGGA 1 cut(s) 146
Bsp143I GATC 1 cut(s) 355
Bsp19I CCATGG 2 cut(s) 64, 524
BspACI CCGC 2 cut(s) 5, 281
BspANI GGCC 2 cut(s) 532, 636
BspCNI CTCAG 4 cut(s) 15, 148, 177, 592
BspEI TCCGGA 1 cut(s) 146
BspPI GGATC 1 cut(s) 350
BspT104I TTCGAA 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 64, 524
BssMI GATC 1 cut(s) 355
BssSI CACGAG 1 cut(s) 55
BssT1I CCWWGG 2 cut(s) 64, 524
Bst2BI CACGAG 1 cut(s) 55
Bst4CI ACNGT 2 cut(s) 344, 559
BstAPI GCANNNNNTGC 1 cut(s) 332
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 394, 597
BstDEI CTNAG 5 cut(s) 23, 156, 164, 503, 600
BstDSI CCRYGG 2 cut(s) 64, 524
BstF5I GGATG 2 cut(s) 238, 577
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 2 cut(s) 332, 626
BstNSI RCATGY 1 cut(s) 599
BstSFI CTRYAG 1 cut(s) 29
BstV1I GCAGC 2 cut(s) 2, 65
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
Bsu36I CCTNAGG 1 cut(s) 164
BsuRI GGCC 2 cut(s) 532, 636
BtgI CCRYGG 2 cut(s) 64, 524
BtsCI GGATG 2 cut(s) 238, 577
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 153
Cac8I GCNNGC 2 cut(s) 394, 597
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 507, 660
Csp6I GTAC 2 cut(s) 378, 555
CviAII CATG 4 cut(s) 65, 495, 525, 596
CviJI RGCY 4 cut(s) 169, 520, 532, 636
CviKI_1 RGCY 4 cut(s) 169, 520, 532, 636
CviQI GTAC 2 cut(s) 378, 555
DdeI CTNAG 5 cut(s) 23, 156, 164, 503, 600
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
DraI TTTAAA 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 634
Eco130I CCWWGG 2 cut(s) 64, 524
Eco147I AGGCCT 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 507, 660
Eco57I CTGAAG 3 cut(s) 357, 492, 536
Eco81I CCTNAGG 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 507
EcoT14I CCWWGG 2 cut(s) 64, 524
ErhI CCWWGG 2 cut(s) 64, 524
FaeI CATG 4 cut(s) 68, 498, 528, 599
FaiI YATR 7 cut(s) 66, 333, 390, 496, 526, 564, 597
FatI CATG 4 cut(s) 64, 494, 524, 595
Fnu4HI GCNGC 2 cut(s) 16, 79
FokI GGATG 2 cut(s) 245, 584
Fsp4HI GCNGC 2 cut(s) 16, 79
FspBI CTAG 1 cut(s) 488
FspI TGCGCA 1 cut(s) 82
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 16, 79
HaeIII GGCC 2 cut(s) 532, 636
HapII CCGG 2 cut(s) 73, 147
HhaI GCGC 1 cut(s) 83
Hin1II CATG 4 cut(s) 68, 498, 528, 599
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HinfI GANTC 6 cut(s) 34, 69, 150, 271, 425, 467
HpaII CCGG 2 cut(s) 73, 147
HphI GGTGA 1 cut(s) 310
Hpy188I TCNGA 4 cut(s) 24, 472, 647, 664
Hpy188III TCNNGA 3 cut(s) 147, 275, 654
HpyAV CCTTC 3 cut(s) 451, 651, 660
HpyCH4III ACNGT 2 cut(s) 344, 559
HpyCH4V TGCA 2 cut(s) 326, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 332, 626
HpyF3I CTNAG 5 cut(s) 23, 156, 164, 503, 600
Hsp92II CATG 4 cut(s) 68, 498, 528, 599
HspAI GCGC 1 cut(s) 81
Kpn2I TCCGGA 1 cut(s) 146
Kzo9I GATC 1 cut(s) 355
LmnI GCTCC 1 cut(s) 525
LpnPI CCDG 9 cut(s) 86, 105, 151, 160, 214, 294, 527, 639, 650
Lsp1109I GCAGC 2 cut(s) 2, 65
MaeI CTAG 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 151, 304
MalI GATC 1 cut(s) 357
MboI GATC 1 cut(s) 355
MboII GAAGA 3 cut(s) 168, 350, 365
MfeI CAATTG 2 cut(s) 435, 621
MflI RGATCY 1 cut(s) 355
MlsI TGGCCA 1 cut(s) 636
MluCI AATT 4 cut(s) 404, 435, 585, 621
MluNI TGGCCA 1 cut(s) 636
MlyI GAGTC 2 cut(s) 159, 419
MmeI TCCRAC 2 cut(s) 383, 587
MnlI CCTC 5 cut(s) 48, 173, 331, 522, 619
Mox20I TGGCCA 1 cut(s) 636
MroI TCCGGA 1 cut(s) 146
MscI TGGCCA 1 cut(s) 636
MseI TTAA 1 cut(s) 582
Msp20I TGGCCA 1 cut(s) 636
MspI CCGG 2 cut(s) 73, 147
MunI CAATTG 2 cut(s) 435, 621
Mva1269I GAATGC 2 cut(s) 298, 312
MwoI GCNNNNNNNGC 2 cut(s) 332, 626
NcoI CCATGG 2 cut(s) 64, 524
NdeII GATC 1 cut(s) 355
NlaIII CATG 4 cut(s) 68, 498, 528, 599
NmuCI GTSAC 2 cut(s) 151, 304
NsbI TGCGCA 1 cut(s) 82
NspI RCATGY 1 cut(s) 599
NspV TTCGAA 1 cut(s) 242
PaeI GCATGC 1 cut(s) 599
PceI AGGCCT 1 cut(s) 532
PctI GAATGC 2 cut(s) 298, 312
PfeI GAWTC 4 cut(s) 34, 69, 271, 467
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 2 cut(s) 17, 80
PleI GAGTC 2 cut(s) 158, 419
PpsI GAGTC 2 cut(s) 158, 419
PpuMI RGGWCCY 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 507
PspPI GGNCC 2 cut(s) 507, 660
PspPPI RGGWCCY 1 cut(s) 507
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 2 cut(s) 379, 556
RsaNI GTAC 2 cut(s) 378, 555
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 2 cut(s) 16, 79
Sau3AI GATC 1 cut(s) 355
Sau96I GGNCC 2 cut(s) 507, 660
SchI GAGTC 2 cut(s) 159, 419
SetI ASST 8 cut(s) 323, 371, 379, 512, 516, 522, 652, 662
SfcI CTRYAG 1 cut(s) 29
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 2 cut(s) 507, 660
SphI GCATGC 1 cut(s) 599
Sse9I AATT 4 cut(s) 404, 435, 585, 621
SseBI AGGCCT 1 cut(s) 532
SsiI CCGC 2 cut(s) 5, 281
SspMI CTAG 1 cut(s) 488
StuI AGGCCT 1 cut(s) 532
StyI CCWWGG 2 cut(s) 64, 524
TaaI ACNGT 2 cut(s) 344, 559
TaqI TCGA 2 cut(s) 242, 276
TasI AATT 4 cut(s) 404, 435, 585, 621
TatI WGTACW 1 cut(s) 554
TfiI GAWTC 4 cut(s) 34, 69, 271, 467
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 2 cut(s) 147, 160
TseFI GTSAC 2 cut(s) 151, 304
TseI GCWGC 2 cut(s) 15, 78
Tsp45I GTSAC 2 cut(s) 151, 304
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 2 cut(s) 147, 160
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 507, 660
XceI RCATGY 1 cut(s) 599
XspI CTAG 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.