FvH4_6g10330

D-ala D-ala ligase N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005073
Physical Location & Seq
Reverse (-)
6151585 .. 6160315
8731 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10330.t1

Sequence Viewer

Length: 2874 bp
ATGGCCTCCGTCGCACTCGGCTCCACCCTCACTTTACTCCGCGCCAACCAAAACGACGCCGCTCCACACAAGCCTCTTATCTTCACTCCAAATTTCAACTCTCTCCGATTCAACCACAGCCTCAGAACTCACGCCTCTTCCACGCGGTGCCGCGGCGTCGGAGCTCCACGCGCCGCCGCCGAGGCGGTGGAGGATGTGATTGTTGAGGGAGTGGAGAAGGAGAAGGTGAAAGGTAGCAGGGCTCTGAGAGTCGGTGTCGTCTGCGGTGGTCCCTCCGCCGAGCGTGGAATCTCTCTCAACTCGGCGAGATCAGTAATCGATCACATACAAGGAGAGGATTTACATGTGAGTTGCTACTACATTGACTCGGAGCTCAATGCGTTTGCGATATCGCCTGCTCAGGTGTACTCCAACACTCCAGCGGATTTTGATTTCAAACTCGAAAGCCTTGCTCAAGGTTTCAAGTCTTGGTCTGACTTTGCGGACCACCTTGCTGTCAATGTGGACATAGTGTTTCCGGTCATACATGGTCAATTTGGTGAAGATGGTGGCATTCAGGAGGTGTTGGAACGGTATAACATTCCATTTGTTGGCACGGGATCAAATGAGTGTCGTCAAGCTTTTGACAAGTACAATGCCTCATTGGAGCTCAGCAGACATGGATTTGTAACTGTACCAAGTTGTTTAGTAGAGGGAAGTGAAGCAGATGAACCTGAACTGTCGGAGTGGTTTGCAAAAAATCAACTGGATCCTAACTCTGGGAAAGTTGTGGTAAAACCAGCACGAGCAGGTTCAAGCATTGGTGTTACAGTTGCATATGGCTTGGCTGATTCTCTTGCCAAGGCTAATGCAATTATTACAGAGGGAATTGATTCCAAAGTCCTTGTTGAAATATTTCTTGAAGGAGGGAGTGAATTTACCGCAATTGTTCTTGATGTGGGGTATGGTACAGATTCTCATCCTGTTGTACTACTACCAACTGAGGTGGAACTTCAATTCCTTGGTAGTGTAGATGTTAGAGAGAAGGATGCAATCTTCAACTATCGCAGGAAATATCTTCCAACACAACAGGTTGCTTATCACACTCCACCTCGTTTTCCTATTGATGTCATCGAAAATATTCGAGATGGAGCATCTCAGTTATTCAAAAGGCTTGGCCTTCGTGACTTTGCTCGAATCGATGGATGGTTCCTGCCTAATTCTGTTCATGTACCGTCATCACCAGATAGCAAGTTTGGAAGGACTGAAATGGGTACAATATTATACACTGACATTAACCTGATTAGTGGTATGGAGCAGACCAGCTTTTTATTCCAGCAAGCCTCAAAGGTTGGGTTTTCTCATGCAAATATCCTCCGGAGTATCATTAATCATGCTTGTTTGAGGTTTCCGCATCTTGCATCTTGTGATGGTGTGTCCGGTGATTTGTCTAGAACATTAAAATCCCCGCTGCTTAAAGATGATTGGGAAGGCACTCAGAAAGTTTTTGTTATTTTTGGAGGAGACACATCCGAGAGGCAAGTATCTCTTATGAGCGGAACAAATGTTTGGCTCAATTTGCAAGCTTTTGATGATCTAGAAGTACTTCCTTGCTTGCTTGCCCCAACGAATGGATATTCATCTAGTAATGATGTGGACAAAAATGAAGTTGATGCAACTTCCAGAACAGTTTGGTCATTACCCTATTCTCTTGTGCTAAGACACACCACGGAGGAAGTTCTTGCTGCATGTGTAGAGGCAATAGAACCAGACCGAGCTGCACTTACATCTCAATTACGGAACAGAGTGATTAATGATCTTATGGAAGGCTTCAAGAAACATTCTTGGTTTACAGGATTCGATATCAATGATGAACTGCCTGTTAAATTTTCTATTGAAGAGTGGATCAAGCTAGCCAAGGAAGTCAAGGCAACAGTATTCATTGCGGTGCATGGAGGTATTGGTGAAGACGGTACACTTCAGTCTTTGCTGGAGGCTGAAGGAGTTCCACATACAGGTCCTGGTGTTTTGGCCTCAAAGATTTGTATGGACAAAGTTGCTACATCAGTTGCTCTTAAACATCTATCAGACTTGGGAGTTCTTACCATAAACAAAGATGTGAGAAGAAGAGATGAGCTCCTTAGTACACCAATACCAAATGTATGGTATGAATTGACCTCCAAGCTTCAGTGTGAAACATTATGTGTTAAACCAGCAAGAGATGGATGCTCAACTGGAGTTGCAAGGTTATGCTGTGACGGAGACCTTTCAGTGTATGTAAAAGCGTTAGAGGACTGTCTCCTTCGGATTCCCCCTAATAGTTTGTCAAAGGAACACGGAATGATCGAAATGCCAAACCCCCCTCCAGAGCTCCTAATCTTTGAACCTTTTATTGAAACGGATGATATAATTGTTTCATCGAAGTCCATGAATGAAAATGGACATCACCTCATGTGGAAAGGACAGAGTAGATGGGTTGAAATAACCATTGGTGTTATTGGGAAACAAGGATTAATGCACTCGTTGAGTCCCAGTATCACCGTAAAGGAGAGTGGTGATATTTTATCACTCGAAGAGAAATTTCAGGGTGGAACTGGCATCAATCTTACTCCACCTCCTTCATCGATCATTAGCCACGAGGCCTTGCAGAAATGCAAACAAAATATAGAAATGATCGCAAACACTCTAGAATTAGAAGGATTTTCAAGAATTGATGCCTTTGTCAACGTGGATAGTGGAGAGGTGCTGATTATAGAGGTCAATACAGTGCCTGGAATGACACCTTCCACTGTTCTTATTCATCAGGCACTAGCAGAGGAACCTCCCATGTATCCTCATCAGTTCTTCCGGACACTGCTTGATTTGGCATCAGAGAGGACCATATACATTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dala_Dala_lig_C Dala_Dala_lig_N

Protein Analysis

958

Amino Acids

104.85

Weight (kDa)

5.12

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dala_Dala_lig_N PF01820 83 - 198 2.4e-24 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 256 - 394 2.4e-13 D-ala D-ala ligase C-terminus
Dala_Dala_lig_N PF01820 494 - 667 1.6e-17 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 819 - 925 2.7e-11 D-ala D-ala ligase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 779
AccB1I GGYRCC 1 cut(s) 147
AccB7I CCANNNNNTGG 2 cut(s) 590, 1610
AccBSI CCGCTC 2 cut(s) 62, 1536
AccII CGCG 4 cut(s) 42, 145, 153, 171
AccIII TCCGGA 2 cut(s) 1356, 2826
AclWI GGATC 4 cut(s) 607, 743, 756, 1892
AcsI RAATTY 4 cut(s) 91, 914, 1865, 2558
AcuI CTGAAG 3 cut(s) 1943, 1998, 2150
AcyI GRCGYC 2 cut(s) 57, 156
AdeI CACNNNGTG 1 cut(s) 147
AfiI CCNNNNNNNGG 4 cut(s) 590, 758, 1610, 1994
AflIII ACRYGT 1 cut(s) 343
AjnI CCWGG 2 cut(s) 1999, 2749
AjuI GAANNNNNNNTTGG 4 cut(s) 1531, 1563, 2451, 2483
AloI GAACNNNNNNTCC 2 cut(s) 981, 1013
Alw21I GWGCWC 5 cut(s) 166, 375, 651, 2118, 2352
Alw26I GTCTC 3 cut(s) 1497, 2235, 2282
AlwI GGATC 4 cut(s) 607, 743, 756, 1892
AlwNI CAGNNNCTG 2 cut(s) 2000, 2750
Aor13HI TCCGGA 2 cut(s) 1356, 2826
AoxI GGCC 4 cut(s) 3, 1156, 2010, 2619
ApeKI GCWGC 3 cut(s) 1450, 1724, 1757
ApoI RAATTY 4 cut(s) 91, 914, 1865, 2558
AseI ATTAAT 3 cut(s) 1368, 1791, 2492
Asp700I GAANNNNTTC 5 cut(s) 871, 894, 1119, 1584, 1983
AspLEI GCGC 2 cut(s) 44, 173
AspS9I GGNCC 4 cut(s) 269, 484, 1997, 2856
AsuHPI GGTGA 8 cut(s) 238, 551, 1212, 1433, 1955, 2417, 2509, 2546
AsuNHI GCTAGC 1 cut(s) 1891
AvaII GGWCC 4 cut(s) 269, 484, 1997, 2856
BamHI GGATCC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 147
BanII GRGCYC 6 cut(s) 166, 244, 375, 651, 2118, 2352
BauI CACGAG 2 cut(s) 783, 2615
BbsI GAAGAC 1 cut(s) 1953
Bbv12I GWGCWC 5 cut(s) 166, 375, 651, 2118, 2352
BbvI GCAGC 3 cut(s) 1437, 1711, 1744
BccI CCATC 7 cut(s) 539, 1121, 1175, 1179, 1403, 2195, 2445
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BciT130I CCWGG 2 cut(s) 2001, 2751
BciVI GTATCC 1 cut(s) 2820
BcoDI GTCTC 3 cut(s) 1497, 2235, 2282
BfaI CTAG 6 cut(s) 1431, 1577, 1623, 1892, 2666, 2789
BfuAI ACCTGC 1 cut(s) 779
BfuI GTATCC 1 cut(s) 2820
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 8 cut(s) 60, 151, 154, 174, 177, 1451, 1725, 1758
BlpI GCTNAGC 1 cut(s) 650
BlsI GCNGC 8 cut(s) 61, 152, 155, 175, 178, 1452, 1726, 1759
BmcAI AGTACT 1 cut(s) 1584
Bme1390I CCNGG 2 cut(s) 2001, 2751
Bme18I GGWCC 4 cut(s) 269, 484, 1997, 2856
BmgT120I GGNCC 4 cut(s) 269, 484, 1997, 2856
BmiI GGNNCC 6 cut(s) 22, 149, 271, 750, 1190, 2799
BmrFI CCNGG 2 cut(s) 2001, 2751
BmrI ACTGGG 1 cut(s) 2505
BmsI GCATC 9 cut(s) 1018, 1142, 1402, 1409, 1642, 2195, 2586, 2683, 2855
BmtI GCTAGC 1 cut(s) 1895
BmuI ACTGGG 1 cut(s) 2505
BpiI GAAGAC 1 cut(s) 1953
BplI GAGNNNNNCTC 2 cut(s) 2100, 2132
BpmI CTGGAG 4 cut(s) 402, 1991, 2235, 2328
Bpu10I CCTNAGC 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 650
BpuEI CTTGAG 1 cut(s) 438
Bsa29I ATCGAT 3 cut(s) 318, 1179, 2603
BsaBI GATNNNNATC 2 cut(s) 957, 1618
BsaHI GRCGYC 2 cut(s) 57, 156
BsaI GGTCTC 1 cut(s) 2235
BsaJI CCNNGG 6 cut(s) 151, 180, 840, 1000, 1707, 1896
BsaWI WCCGGW 4 cut(s) 517, 1356, 1418, 2826
BsaXI ACNNNNNCTCC 2 cut(s) 2578, 2608
Bsc4I CCNNNNNNNGG 4 cut(s) 590, 758, 1610, 1994
Bse1I ACTGG 4 cut(s) 750, 2218, 2511, 2578
Bse3DI GCAATG 1 cut(s) 1920
Bse8I GATNNNNATC 2 cut(s) 957, 1618
BseAI TCCGGA 2 cut(s) 1356, 2826
BseBI CCWGG 2 cut(s) 2001, 2751
BseCI ATCGAT 3 cut(s) 318, 1179, 2603
BseDI CCNNGG 6 cut(s) 151, 180, 840, 1000, 1707, 1896
BseGI GGATG 7 cut(s) 199, 958, 1033, 1190, 1508, 2210, 2386
BseJI GATNNNNATC 2 cut(s) 957, 1618
BseLI CCNNNNNNNGG 4 cut(s) 590, 758, 1610, 1994
BseMI GCAATG 1 cut(s) 1920
BseMII CTCAG 7 cut(s) 136, 236, 413, 664, 972, 1151, 1490
BseNI ACTGG 4 cut(s) 750, 2218, 2511, 2578
BseRI GAGGAG 1 cut(s) 1515
BseXI GCAGC 3 cut(s) 1437, 1711, 1744
BsgI GTGCAG 1 cut(s) 1743
Bsh1236I CGCG 4 cut(s) 42, 145, 153, 171
BshFI GGCC 4 cut(s) 5, 1158, 2012, 2621
BshNI GGYRCC 1 cut(s) 147
BshVI ATCGAT 3 cut(s) 318, 1179, 2603
BsiHKAI GWGCWC 5 cut(s) 166, 375, 651, 2118, 2352
BsiSI CCGG 4 cut(s) 518, 1357, 1419, 2827
BslFI GGGAC 2 cut(s) 255, 2493
BslI CCNNNNNNNGG 4 cut(s) 590, 758, 1610, 1994
BsmAI GTCTC 3 cut(s) 1497, 2235, 2282
BsmFI GGGAC 2 cut(s) 255, 2493
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 4 cut(s) 5, 1158, 2012, 2621
Bso31I GGTCTC 1 cut(s) 2235
Bsp1286I GDGCHC 6 cut(s) 166, 244, 375, 651, 2118, 2352
Bsp13I TCCGGA 2 cut(s) 1356, 2826
Bsp1720I GCTNAGC 1 cut(s) 650
BspANI GGCC 4 cut(s) 5, 1158, 2012, 2621
BspCNI CTCAG 7 cut(s) 135, 237, 412, 663, 973, 1150, 1489
BspDI ATCGAT 3 cut(s) 318, 1179, 2603
BspEI TCCGGA 2 cut(s) 1356, 2826
BspFNI CGCG 4 cut(s) 42, 145, 153, 171
BspLI GGNNCC 6 cut(s) 22, 149, 271, 750, 1190, 2799
BspMI ACCTGC 1 cut(s) 779
BspOI GCTAGC 1 cut(s) 1895
BspPI GGATC 4 cut(s) 607, 743, 756, 1892
BspT107I GGYRCC 1 cut(s) 147
BspTNI GGTCTC 1 cut(s) 2235
BsrBI CCGCTC 2 cut(s) 62, 1536
BsrDI GCAATG 1 cut(s) 1920
BsrI ACTGG 4 cut(s) 750, 2218, 2511, 2578
BssECI CCNNGG 6 cut(s) 151, 180, 840, 1000, 1707, 1896
BssNI GRCGYC 2 cut(s) 57, 156
BssSI CACGAG 2 cut(s) 783, 2615
BssT1I CCWWGG 3 cut(s) 840, 1000, 1896
Bst2BI CACGAG 2 cut(s) 783, 2615
Bst2UI CCWGG 2 cut(s) 2001, 2751
Bst6I CTCTTC 4 cut(s) 142, 1872, 2101, 2547
BstACI GRCGYC 2 cut(s) 57, 156
BstC8I GCNNGC 6 cut(s) 396, 1320, 1563, 1595, 1599, 1893
BstDEI CTNAG 9 cut(s) 122, 245, 399, 650, 981, 1137, 1476, 1697, 2120
BstDSI CCRYGG 2 cut(s) 151, 1707
BstF5I GGATG 7 cut(s) 199, 958, 1033, 1190, 1508, 2210, 2386
BstFNI CGCG 4 cut(s) 42, 145, 153, 171
BstHHI GCGC 2 cut(s) 44, 173
BstMAI GTCTC 3 cut(s) 1497, 2235, 2282
BstMWI GCNNNNNNNGC 4 cut(s) 11, 170, 182, 1558
BstNI CCWGG 2 cut(s) 2001, 2751
BstNSI RCATGY 2 cut(s) 347, 1731
BstSCI CCNGG 2 cut(s) 1999, 2749
BstUI CGCG 4 cut(s) 42, 145, 153, 171
BstV1I GCAGC 3 cut(s) 1437, 1711, 1744
BstV2I GAAGAC 1 cut(s) 1953
BstX2I RGATCY 1 cut(s) 748
BstXI CCANNNNNNTGG 1 cut(s) 2142
BstYI RGATCY 1 cut(s) 748
Bsu15I ATCGAT 3 cut(s) 318, 1179, 2603
BsuI GTATCC 1 cut(s) 2820
BsuRI GGCC 4 cut(s) 5, 1158, 2012, 2621
BsuTUI ATCGAT 3 cut(s) 318, 1179, 2603
BtgI CCRYGG 2 cut(s) 151, 1707
BtsCI GGATG 7 cut(s) 199, 958, 1033, 1190, 1508, 2210, 2386
BtsI GCAGTG 1 cut(s) 2831
BtsIMutI CAGTG 6 cut(s) 1266, 2174, 2256, 2751, 2766, 2831
BveI ACCTGC 1 cut(s) 779
Cac8I GCNNGC 6 cut(s) 396, 1320, 1563, 1595, 1599, 1893
CaiI CAGNNNCTG 2 cut(s) 2000, 2750
CfoI GCGC 2 cut(s) 44, 173
Cfr13I GGNCC 4 cut(s) 269, 484, 1997, 2856
Cfr42I CCGCGG 1 cut(s) 154
ClaI ATCGAT 3 cut(s) 318, 1179, 2603
CseI GACGC 2 cut(s) 65, 145
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
DdeI CTNAG 9 cut(s) 122, 245, 399, 650, 981, 1137, 1476, 1697, 2120
DraIII CACNNNGTG 1 cut(s) 147
Eam1104I CTCTTC 4 cut(s) 142, 1872, 2101, 2547
EarI CTCTTC 4 cut(s) 142, 1872, 2101, 2547
EciI GGCGGA 1 cut(s) 265
Ecl136II GAGCTC 5 cut(s) 164, 373, 649, 2116, 2350
Eco130I CCWWGG 3 cut(s) 840, 1000, 1896
Eco147I AGGCCT 1 cut(s) 2621
Eco24I GRGCYC 6 cut(s) 166, 244, 375, 651, 2118, 2352
Eco31I GGTCTC 1 cut(s) 2235
Eco32I GATATC 2 cut(s) 390, 1843
Eco47I GGWCC 4 cut(s) 269, 484, 1997, 2856
Eco53kI GAGCTC 5 cut(s) 164, 373, 649, 2116, 2350
Eco57I CTGAAG 3 cut(s) 1943, 1998, 2150
EcoICRI GAGCTC 5 cut(s) 164, 373, 649, 2116, 2350
EcoO109I RGGNCCY 1 cut(s) 1997
EcoRII CCWGG 2 cut(s) 1999, 2749
EcoRV GATATC 2 cut(s) 390, 1843
EcoT14I CCWWGG 3 cut(s) 840, 1000, 1896
EcoT38I GRGCYC 6 cut(s) 166, 244, 375, 651, 2118, 2352
ErhI CCWWGG 3 cut(s) 840, 1000, 1896
FalI AAGNNNNNCTT 2 cut(s) 1512, 1544
FaqI GGGAC 2 cut(s) 255, 2493
FauI CCCGC 1 cut(s) 1455
FauNDI CATATG 1 cut(s) 817
Fnu4HI GCNGC 8 cut(s) 60, 151, 154, 174, 177, 1451, 1725, 1758
FokI GGATG 7 cut(s) 206, 945, 1040, 1197, 1495, 2217, 2393
FriOI GRGCYC 6 cut(s) 166, 244, 375, 651, 2118, 2352
Fsp4HI GCNGC 8 cut(s) 60, 151, 154, 174, 177, 1451, 1725, 1758
FspBI CTAG 6 cut(s) 1431, 1577, 1623, 1892, 2666, 2789
GlaI GCGC 2 cut(s) 43, 172
GluI GCNGC 8 cut(s) 60, 151, 154, 174, 177, 1451, 1725, 1758
GsuI CTGGAG 4 cut(s) 402, 1991, 2235, 2328
HaeIII GGCC 4 cut(s) 5, 1158, 2012, 2621
HapII CCGG 4 cut(s) 518, 1357, 1419, 2827
HgaI GACGC 2 cut(s) 65, 145
HhaI GCGC 2 cut(s) 44, 173
Hin1I GRCGYC 2 cut(s) 57, 156
Hin6I GCGC 2 cut(s) 42, 171
HinP1I GCGC 2 cut(s) 42, 171
HincII GTYRAC 1 cut(s) 2704
HindII GTYRAC 1 cut(s) 2704
HindIII AAGCTT 3 cut(s) 618, 1563, 2162
HpaII CCGG 4 cut(s) 518, 1357, 1419, 2827
HphI GGTGA 8 cut(s) 238, 551, 1212, 1433, 1955, 2417, 2509, 2546
Hpy166II GTNNAC 7 cut(s) 406, 505, 1636, 1830, 1955, 2126, 2704
Hpy8I GTNNAC 7 cut(s) 406, 505, 1636, 1830, 1955, 2126, 2704
Hpy99I CGWCG 3 cut(s) 14, 59, 161
HpyCH4IV ACGT 1 cut(s) 2706
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 170, 182, 1558
HpyF3I CTNAG 9 cut(s) 122, 245, 399, 650, 981, 1137, 1476, 1697, 2120
HpySE526I ACGT 1 cut(s) 2706
Hsp92I GRCGYC 2 cut(s) 57, 156
HspAI GCGC 2 cut(s) 42, 171
Kpn2I TCCGGA 2 cut(s) 1356, 2826
KspI CCGCGG 1 cut(s) 154
Lsp1109I GCAGC 3 cut(s) 1437, 1711, 1744
LweI GCATC 9 cut(s) 1018, 1142, 1402, 1409, 1642, 2195, 2586, 2683, 2855
MaeI CTAG 6 cut(s) 1431, 1577, 1623, 1892, 2666, 2789
MaeII ACGT 1 cut(s) 2706
MaeIII GTNAC 4 cut(s) 667, 805, 1163, 2234
MbiI CCGCTC 2 cut(s) 62, 1536
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 1 cut(s) 748
MhlI GDGCHC 6 cut(s) 166, 244, 375, 651, 2118, 2352
MlyI GAGTC 3 cut(s) 258, 359, 2515
MmeI TCCRAC 5 cut(s) 139, 435, 546, 702, 1085
MroI TCCGGA 2 cut(s) 1356, 2826
MroXI GAANNNNTTC 5 cut(s) 871, 894, 1119, 1584, 1983
MseI TTAA 9 cut(s) 1275, 1368, 1439, 1455, 1791, 1863, 2055, 2187, 2492
MslI CAYNNNNRTG 1 cut(s) 1925
MspA1I CMGCKG 3 cut(s) 153, 422, 1450
MspI CCGG 4 cut(s) 518, 1357, 1419, 2827
MspR9I CCNGG 2 cut(s) 2001, 2751
MunI CAATTG 1 cut(s) 924
Mva1269I GAATGC 1 cut(s) 552
MvaI CCWGG 2 cut(s) 2001, 2751
MvnI CGCG 4 cut(s) 42, 145, 153, 171
MwoI GCNNNNNNNGC 4 cut(s) 11, 170, 182, 1558
NdeI CATATG 1 cut(s) 817
NheI GCTAGC 1 cut(s) 1891
NlaIV GGNNCC 6 cut(s) 22, 149, 271, 750, 1190, 2799
NmeAIII GCCGAG 3 cut(s) 205, 281, 304
NmuCI GTSAC 2 cut(s) 1163, 2234
NspI RCATGY 2 cut(s) 347, 1731
PceI AGGCCT 1 cut(s) 2621
PciI ACATGT 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 2322
PctI GAATGC 1 cut(s) 552
PdmI GAANNNNTTC 5 cut(s) 871, 894, 1119, 1584, 1983
PfeI GAWTC 8 cut(s) 108, 288, 830, 872, 953, 1176, 1836, 2287
PflMI CCANNNNNTGG 2 cut(s) 590, 1610
PkrI GCNGC 8 cut(s) 61, 152, 155, 175, 178, 1452, 1726, 1759
PleI GAGTC 3 cut(s) 257, 359, 2514
PpsI GAGTC 3 cut(s) 257, 359, 2514
PpuMI RGGWCCY 1 cut(s) 1997
PscI ACATGT 1 cut(s) 343
PshBI ATTAAT 3 cut(s) 1368, 1791, 2492
Psp124BI GAGCTC 5 cut(s) 166, 375, 651, 2118, 2352
Psp5II RGGWCCY 1 cut(s) 1997
Psp6I CCWGG 2 cut(s) 1999, 2749
PspGI CCWGG 2 cut(s) 1999, 2749
PspN4I GGNNCC 6 cut(s) 22, 149, 271, 750, 1190, 2799
PspPI GGNCC 4 cut(s) 269, 484, 1997, 2856
PspPPI RGGWCCY 1 cut(s) 1997
PstNI CAGNNNCTG 2 cut(s) 2000, 2750
PsuI RGATCY 1 cut(s) 748
RseI CAYNNNNRTG 1 cut(s) 1925
SacI GAGCTC 5 cut(s) 166, 375, 651, 2118, 2352
SacII CCGCGG 1 cut(s) 154
SaqAI TTAA 9 cut(s) 1275, 1368, 1439, 1455, 1791, 1863, 2055, 2187, 2492
SatI GCNGC 8 cut(s) 60, 151, 154, 174, 177, 1451, 1725, 1758
Sau96I GGNCC 4 cut(s) 269, 484, 1997, 2856
ScaI AGTACT 1 cut(s) 1584
SchI GAGTC 3 cut(s) 258, 359, 2515
ScrFI CCNGG 2 cut(s) 2001, 2751
SduI GDGCHC 6 cut(s) 166, 244, 375, 651, 2118, 2352
SfaNI GCATC 9 cut(s) 1018, 1142, 1402, 1409, 1642, 2195, 2586, 2683, 2855
Sfr303I CCGCGG 1 cut(s) 154
SgrBI CCGCGG 1 cut(s) 154
SinI GGWCC 4 cut(s) 269, 484, 1997, 2856
SmiMI CAYNNNNRTG 1 cut(s) 1925
SmlI CTYRAG 1 cut(s) 453
SmoI CTYRAG 1 cut(s) 453
SseBI AGGCCT 1 cut(s) 2621
SspI AATATT 3 cut(s) 894, 1120, 1260
SspMI CTAG 6 cut(s) 1431, 1577, 1623, 1892, 2666, 2789
SstI GAGCTC 5 cut(s) 166, 375, 651, 2118, 2352
StuI AGGCCT 1 cut(s) 2621
StyD4I CCNGG 2 cut(s) 1999, 2749
StyI CCWWGG 3 cut(s) 840, 1000, 1896
TaiI ACGT 1 cut(s) 2709
TaqII GACCGA 1 cut(s) 1767
TatI WGTACW 5 cut(s) 405, 630, 967, 1582, 2123
TauI GCSGC 5 cut(s) 62, 153, 156, 176, 179
TfiI GAWTC 8 cut(s) 108, 288, 830, 872, 953, 1176, 1836, 2287
Tru1I TTAA 9 cut(s) 1275, 1368, 1439, 1455, 1791, 1863, 2055, 2187, 2492
Tru9I TTAA 9 cut(s) 1275, 1368, 1439, 1455, 1791, 1863, 2055, 2187, 2492
TscAI CASTG 6 cut(s) 1273, 2174, 2256, 2751, 2773, 2838
TseFI GTSAC 2 cut(s) 1163, 2234
TseI GCWGC 3 cut(s) 1450, 1724, 1757
Tsp45I GTSAC 2 cut(s) 1163, 2234
TspGWI ACGGA 5 cut(s) 1724, 1792, 2253, 2331, 2393
TspRI CASTG 6 cut(s) 1273, 2174, 2256, 2751, 2773, 2838
Van91I CCANNNNNTGG 2 cut(s) 590, 1610
VpaK11BI GGWCC 4 cut(s) 269, 484, 1997, 2856
VspI ATTAAT 3 cut(s) 1368, 1791, 2492
XapI RAATTY 4 cut(s) 91, 914, 1865, 2558
XbaI TCTAGA 3 cut(s) 1430, 1576, 2665
XceI RCATGY 2 cut(s) 347, 1731
XmnI GAANNNNTTC 5 cut(s) 871, 894, 1119, 1584, 1983
XspI CTAG 6 cut(s) 1431, 1577, 1623, 1892, 2666, 2789
ZrmI AGTACT 1 cut(s) 1584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.