Rorug03G0048200

D-ala D-ala ligase N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005073
Physical Location & Seq
Reverse (-)
3745201 .. 3746435
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0048200.1

Sequence Viewer

Length: 426 bp
ATGAGTGGGAAGGAAGGTGGGTCGACTAAGGGAGGCAGAGGCAAGCCGAAGGCCACCAAGTCTGTGTCCAGGTCTAGCAAAGCGGGGCTTCAATTTCCGGTGGGTAGGATCGCCCGGTTTCTCAAGTCCGGCAAGTATGCCGAGCGTGTCGGCGCCGGAGCTCCGGTTTATCTCTCTGCCGTGCTTGAATACCTGGCGGCTGAGGTTTTGGAGCTTGCTGGCAACGCAGCTAGGGACAACAAGAAGAATCGTATTGTACCGAGGCACATTCAGCTTGCGGTGAGGAATGACGAGGAGCTAAGCAAGCTTTTGGGGTCTGTGACTATTGCTAATGGAGGAGTTTTGCCCAACATTCACCAAACTCTTTTGCCTAAGAAGGTTGGGAAAGGCAAGGGCGATATCGGATCCGCTTCTCAGGAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dala_Dala_lig_C Dala_Dala_lig_N

Protein Analysis

141

Amino Acids

14.79

Weight (kDa)

10.28

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 16 - 95 7.9e-15 Core histone H2A/H2B/H3/H4 domain
Histone_H2A_C PF16211 98 - 131 2.4e-18 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 152
AccI GTMKAC 1 cut(s) 23
AciI CCGC 4 cut(s) 83, 197, 278, 408
AclWI GGATC 3 cut(s) 116, 399, 412
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 1 cut(s) 258
AgsI TTSAA 2 cut(s) 92, 188
AjnI CCWGG 2 cut(s) 68, 192
AluBI AGCT 6 cut(s) 161, 214, 230, 274, 298, 307
AluI AGCT 6 cut(s) 161, 214, 230, 274, 298, 307
Alw21I GWGCWC 1 cut(s) 163
AlwI GGATC 3 cut(s) 116, 399, 412
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 227
AspLEI GCGC 1 cut(s) 155
AsuC2I CCSGG 1 cut(s) 115
AsuHPI GGTGA 2 cut(s) 292, 347
BamHI GGATCC 1 cut(s) 404
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 163
Bbv12I GWGCWC 1 cut(s) 163
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 239
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 2 cut(s) 70, 194
BcnI CCSGG 1 cut(s) 115
BfaI CTAG 3 cut(s) 75, 231, 424
BfoI RGCGCY 1 cut(s) 156
BisI GCNGC 2 cut(s) 198, 228
BlpI GCTNAGC 1 cut(s) 299
BlsI GCNGC 2 cut(s) 199, 229
Bme1390I CCNGG 3 cut(s) 70, 115, 194
BmiI GGNNCC 2 cut(s) 154, 406
BmrFI CCNGG 3 cut(s) 70, 115, 194
Bpu10I CCTNAGC 1 cut(s) 201
Bpu1102I GCTNAGC 1 cut(s) 299
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 115
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 260
BsaWI WCCGGW 2 cut(s) 97, 163
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
BseBI CCWGG 2 cut(s) 70, 194
BseDI CCNNGG 1 cut(s) 260
BseMII CTCAG 1 cut(s) 192
BseRI GAGGAG 2 cut(s) 308, 351
BseXI GCAGC 1 cut(s) 239
BshFI GGCC 1 cut(s) 53
BshNI GGYRCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 5 cut(s) 98, 115, 129, 156, 164
BslFI GGGAC 1 cut(s) 248
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 2 cut(s) 108, 404
Bsp1720I GCTNAGC 1 cut(s) 299
BspACI CCGC 4 cut(s) 83, 197, 278, 408
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 1 cut(s) 193
BspLI GGNNCC 2 cut(s) 154, 406
BspPI GGATC 3 cut(s) 116, 399, 412
BspT107I GGYRCC 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 2 cut(s) 108, 404
BssNI GRCGYC 1 cut(s) 153
Bst2UI CCWGG 2 cut(s) 70, 194
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 5 cut(s) 44, 216, 220, 276, 305
BstDEI CTNAG 5 cut(s) 27, 201, 299, 372, 414
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 2 cut(s) 111, 407
BstMBI GATC 2 cut(s) 108, 404
BstMWI GCNNNNNNNGC 3 cut(s) 224, 271, 304
BstNI CCWGG 2 cut(s) 70, 194
BstSCI CCNGG 3 cut(s) 68, 113, 192
BstV1I GCAGC 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 404
BstYI RGATCY 1 cut(s) 404
BsuRI GGCC 1 cut(s) 53
Cac8I GCNNGC 5 cut(s) 44, 216, 220, 276, 305
CfoI GCGC 1 cut(s) 155
Csp6I GTAC 1 cut(s) 257
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 5 cut(s) 27, 201, 299, 372, 414
DinI GGCGCC 1 cut(s) 154
DpnI GATC 2 cut(s) 110, 406
DpnII GATC 2 cut(s) 108, 404
Ecl136II GAGCTC 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 163
Eco32I GATATC 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 68, 192
EcoRV GATATC 1 cut(s) 400
EcoT38I GRGCYC 1 cut(s) 163
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
FaiI YATR 1 cut(s) 138
FalI AAGNNNNNCTT 2 cut(s) 72, 104
FaqI GGGAC 1 cut(s) 248
FauI CCCGC 1 cut(s) 76
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 2 cut(s) 198, 228
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 2 cut(s) 198, 228
FspBI CTAG 3 cut(s) 75, 231, 424
GlaI GCGC 1 cut(s) 154
GluI GCNGC 2 cut(s) 198, 228
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 1 cut(s) 53
HapII CCGG 5 cut(s) 98, 115, 129, 156, 164
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 153
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 1 cut(s) 247
HpaII CCGG 5 cut(s) 98, 115, 129, 156, 164
HphI GGTGA 2 cut(s) 292, 347
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 1 cut(s) 404
Hpy188III TCNNGA 1 cut(s) 416
Hpy8I GTNNAC 1 cut(s) 24
HpyAV CCTTC 4 cut(s) 4, 8, 43, 370
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 271, 304
HpyF3I CTNAG 5 cut(s) 27, 201, 299, 372, 414
Hsp92I GRCGYC 1 cut(s) 153
HspAI GCGC 1 cut(s) 153
KasI GGCGCC 1 cut(s) 152
Kzo9I GATC 2 cut(s) 108, 404
LmnI GCTCC 4 cut(s) 158, 166, 211, 295
Lsp1109I GCAGC 1 cut(s) 239
MaeI CTAG 3 cut(s) 75, 231, 424
MaeIII GTNAC 1 cut(s) 319
MalI GATC 2 cut(s) 110, 406
MboI GATC 2 cut(s) 108, 404
MboII GAAGA 1 cut(s) 256
MflI RGATCY 1 cut(s) 404
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 1 cut(s) 92
Mly113I GGCGCC 1 cut(s) 153
MnlI CCTC 7 cut(s) 26, 32, 196, 255, 276, 286, 329
MspI CCGG 5 cut(s) 98, 115, 129, 156, 164
MspR9I CCNGG 3 cut(s) 70, 115, 194
MvaI CCWGG 2 cut(s) 70, 194
MwoI GCNNNNNNNGC 3 cut(s) 224, 271, 304
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 1 cut(s) 115
NdeII GATC 2 cut(s) 108, 404
NlaIV GGNNCC 2 cut(s) 154, 406
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 319
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 2 cut(s) 199, 229
PluTI GGCGCC 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 163
Psp6I CCWGG 2 cut(s) 68, 192
PspGI CCWGG 2 cut(s) 68, 192
PspN4I GGNNCC 2 cut(s) 154, 406
PsuI RGATCY 1 cut(s) 404
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
SacI GAGCTC 1 cut(s) 163
SalI GTCGAC 1 cut(s) 22
SatI GCNGC 2 cut(s) 198, 228
Sau3AI GATC 2 cut(s) 108, 404
ScrFI CCNGG 3 cut(s) 70, 115, 194
SduI GDGCHC 1 cut(s) 163
SfoI GGCGCC 1 cut(s) 154
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 92
SsiI CCGC 4 cut(s) 83, 197, 278, 408
SspDI GGCGCC 1 cut(s) 152
SspMI CTAG 3 cut(s) 75, 231, 424
SstI GAGCTC 1 cut(s) 163
StyD4I CCNGG 3 cut(s) 68, 113, 192
TaqI TCGA 1 cut(s) 23
TasI AATT 1 cut(s) 92
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 1 cut(s) 247
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 1 cut(s) 227
Tsp45I GTSAC 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 23
XspI CTAG 3 cut(s) 75, 231, 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.