Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0005073

Physical Location & Seq

Genomic Coordinates Reverse (-)

8898700 .. 8906937

Gene Structure 8238 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG111400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2634 bp

ATGGCCAGCCTCGCATTCGGTTCCAGCCTCACTTTACTTCGCGCCAACCAAAACGACGCCGCACAGAATAAGCCTCTTATCTACACTCCCAATTTCAACTCTCTCCGATTCAACCACAGCCTCAGGACCCAAGCTTCGTCGTCGACGCGGTGCCGCGGCGTCGGAGCTCCACGCGCCGCCGCGGAGGTGTTGGAGGATGTAACTGTCGAGGGAGAGGAGAAGGAGAATCGTAGGGTTCTGAGAGTCGGGGTCATCTGCGGTGGCCCCTCCGCCGAGCGTGGAATCTCTCTCAACTCCGCTAGATCAGTAATCGATCATATTCAAGGAGAGGATTTACATGTGAGTTGCTACTACATTGACTCTGAACTCAACGCTTTTGCAATTTCCCCTGCTCAGGTGTACTCCAATACTCCGGCGGATTTTGATTTCAAACTCGAAAGCCTTGCTCAAAGTTTCAAGTCTTGGTCTGACTTTGCGGACCATCTTGCTGTCAATGTGGACATAGTGTTTCCAGTCATACATGGTCAATTTGGTGAAGATGGCGGCATTCAGGAGGTGTTGGAAAGGTATAACATTCCATTTGTTGGCACGGGATCAAATGAGTGTCGTCAAGCATTCGACAAGTACAATGCCTCATTGGAGCTCAGCAGACATGGATTCGTAACTGTACCAAGTTGTTTAGTACAGGGAAGTAAAGCAGATGAACCTGAACTGTCGGAATGGTTTGCAAAAAATCAACTGGATCCTAAGTCCGGAAAAGTTGTGGTAAAACCAGCACGAGCAGGTTCAAGCATTGGTGTTACAGTTGCATATGGCTTGGCTGATTCTCTTAGCAAGGCTAATGCAATTATTACAGAGGGAATTGATTCCAAAGTCCTTGTTGAAATATTTCTTGAAGGAGGGAGTGAATTTACAGCAATTGTTCTTGATGTGGGGTATGGTACAGATTCTCATCCTGTTGTACTATTACCAACTGAGGTGGAACTTCAATTCCTTGGTAGTGTAGATGTTAGAGAGAAGGATGCAATCTTCAACTACCGCAGGAAATATCTTCCAACACAACAGGTTGCTTATCATACTCCACCTCGTTTTCCTATTGATGTCATCCAAAATATTCGAGATGGAGCATCTCAGTTATTCCAAAAGCTTGGCCTTCGTGACTTTGCTCGAATCGATGGATGGTTTCTGCCTAATTCTATTCATGTACCATCATCGCCAGATAGCAAGTTTGGAAGGACTGAGATGGGCACAATATTATACACTGACATTAACCTGATTAGTGGTATGGAGCAGACCAGCTTTTTATTCCAGCAAGCCTCAAAGGTTGGGTTTTCTCATGCAAATATTCTCCGGAGCATCATTAATCATGCTTGTTTGCGATTTCCGCATCTTGCATCTTGTGATGTGTCTAGTGATTTGCCTAGAACATCAAAATCCCCACTGCTTAAAGATGATTGGGAAGGCACTCAGAAAGTTTTTGTTATTTTTGGAGGAGACACGTCAGAGAGGCAAGTATCTCTTATGAGCGGAACAAATGTTTGGCTCAATTTGCAAGCTTTTGATGATCTGGAAGTGCTTCCTTGCTTGCTTGCCCCCACGAATGGATATTCGTCTAGTACTGATGTGGACAAAAATGAAGTTGATGCAACTTCCCAAACAGTTTGGTCATTACCTTATTCTCTTGTGCTAAGACACACCACGGAGGAAGTTCTTGCTGCATGTGTAGAGGCAATAGAACCAGACCGAGCTGCACTTACATCTCAATTACGGAACAGAGTTATTGATGATCTTATGGAAGGCTTCAAGAAACATTCTTGGTTTACAGGATTTGATATCACTGATGAACTGCCTGTTAAATTTTCTATTGAAGAGTGGATAAAGCTAGCCAAGGAAGTCAAGGCAACAGTATTTATTGCAGTGCATGGAGGCATTGGTGAAGATGGTACACTTCAGTCTTTGTTGGAGGCTGAAGGGATTCCACATACAGGTCCCGGTGTTTTGGCTTCAAAGATTTGTATGGACAAAGTCGCAACATCAGTTGCTCTTAAACATCTATCGGACTTGGGAGTTCTTACCATAAACAAAGATTTGAGAAGAAGAGATCAGCTCCTTAATACACCAATACCAAATGTATGGCATGAATTGACCTCCAAGCTTCAGTGTGAAACATTATGTGTTAAACCGGCAAGAGATGGATGCTCAACTGGAGTTGCAAGGTTATGCTGTGATGAAGACCTTTCAGTGTATGTAAAAGCATTGGAGGACTGTCTCCTTCGGATTCCCCCTAATAGTTTGTCAAAGGAACACGGAATGATCGAAATGCCAAACCCCCCTCCAGAGCTCCTAATCTTTGAACCTTTTATTGATACGGATGATATAATTGTTTCATTGAAGTCCATAAATGAAAATGGACATCACCTCATGTGGAATGGACAGAGTAGATGGGTTGAAATAACTGTTGGTGTTATTGGGAAACAAGGATCAATGCACTCATTGAGTCCCAGTATCACTGTGAAGGAGAGTGGTGATATTTTATCACTTGAGGAGAAATTTCAGGGTGGGACTGGCATCAATCTTACTCCACCTCCGTCATCAATCATTAGGTTTGTCAATAAAAATGATTCCTTTTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dala_Dala_lig_C Dala_Dala_lig_N

Protein Analysis

877

Amino Acids

96.3

Weight (kDa)

5.17

Isoelectric Point (pI)

42.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dala_Dala_lig_N	PF01820	81 - 196	1.4e-24	D-ala D-ala ligase N-terminus
Dala_Dala_lig_C	PF07478	254 - 392	2.9e-13	D-ala D-ala ligase C-terminus
Dala_Dala_lig_N	PF01820	491 - 664	9.2e-18	D-ala D-ala ligase N-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005073)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08840 AT3G08840 AT3G08840 AT3G08840 AT3G08840 AT3G08840 AT3G08840 AT3G08840 AT3G08840 AT3G08840 AT3G08840 AT3G08840 AT3G08840
fragaria_vesca	FvH4_6g10330
malus_domestica	MD04G1168900.v1.1 MD12G1182400.v1.1
prunus_persica	Prupe.6G287100_v2.0.a1
pyrus_communis	pycom04g14970 pycom12g17150
rosa_chinensis	RchiOBHm_Chr3g0461101
rosa_laevigata	RLG00000024952
rosa_multiflora	Rmu_sc0001521.1_g000056
rosa_roxburghii	Rroxscaffold_6G00418850
rosa_rugosa	Rorug03G0048200 Rorug03G0048300
rosa_samantha	Rh3AG105700 Rh3BG109700 Rh3CG111400 Rh3DG111400
rosa_wichuraiana	Rw3G009050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	773
AccB1I	GGYRCC	1 cut(s)	150
AccB7I	CCANNNNNTGG	1 cut(s)	584
AccBSI	CCGCTC	1 cut(s)	1527
AccI	GTMKAC	1 cut(s)	143
AccII	CGCG	5 cut(s)	42, 148, 156, 174, 182
AccIII	TCCGGA	2 cut(s)	752, 1350
AclWI	GGATC	4 cut(s)	601, 737, 750, 2488
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	3 cut(s)	908, 1856, 2549
AcuI	CTGAAG	3 cut(s)	1934, 1989, 2141
AcyI	GRCGYC	2 cut(s)	57, 159
AfaI	GTAC	9 cut(s)	401, 626, 669, 684, 943, 963, 1206, 1618, 1945
AfiI	CCNNNNNNNGG	5 cut(s)	394, 584, 752, 1601, 1985
AflIII	ACRYGT	2 cut(s)	337, 1497
AjiI	CACGTC	1 cut(s)	1500
AjuI	GAANNNNNNNTTGG	4 cut(s)	1522, 1554, 2442, 2474
AloI	GAACNNNNNNTCC	2 cut(s)	975, 1007
Alw21I	GWGCWC	3 cut(s)	169, 645, 2343
Alw26I	GTCTC	2 cut(s)	1488, 2273
AlwI	GGATC	4 cut(s)	601, 737, 750, 2488
Aor13HI	TCCGGA	2 cut(s)	752, 1350
AoxI	GGCC	3 cut(s)	3, 262, 1150
ApeKI	GCWGC	2 cut(s)	1715, 1748
ApoI	RAATTY	3 cut(s)	908, 1856, 2549
ArsI	GACNNNNNNTTYG	2 cut(s)	118, 150
AseI	ATTAAT	1 cut(s)	1362
Asp700I	GAANNNNTTC	4 cut(s)	865, 888, 1575, 1974
AspLEI	GCGC	2 cut(s)	44, 176
AspS9I	GGNCC	4 cut(s)	126, 263, 478, 1988
AsuC2I	CCSGG	1 cut(s)	1992
AsuHPI	GGTGA	4 cut(s)	545, 1946, 2408, 2537
AsuNHI	GCTAGC	1 cut(s)	1882
AvaII	GGWCC	3 cut(s)	126, 478, 1988
AxyI	CCTNAGG	1 cut(s)	122
BaeGI	GKGCMC	1 cut(s)	1250
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	742
BanI	GGYRCC	1 cut(s)	150
BanII	GRGCYC	3 cut(s)	169, 645, 2343
BauI	CACGAG	1 cut(s)	777
BbsI	GAAGAC	1 cut(s)	2238
Bbv12I	GWGCWC	3 cut(s)	169, 645, 2343
BbvI	GCAGC	2 cut(s)	1702, 1735
BcgI	CGANNNNNNTGC	2 cut(s)	425, 459
BcnI	CCSGG	1 cut(s)	1992
BcoDI	GTCTC	2 cut(s)	1488, 2273
BfaI	CTAG	5 cut(s)	300, 1410, 1422, 1614, 1883
BfuAI	ACCTGC	1 cut(s)	773
BisI	GCNGC	8 cut(s)	60, 154, 157, 177, 180, 544, 1716, 1749
BlpI	GCTNAGC	1 cut(s)	644
BlsI	GCNGC	8 cut(s)	61, 155, 158, 178, 181, 545, 1717, 1750
BmcAI	AGTACT	1 cut(s)	1618
Bme1390I	CCNGG	1 cut(s)	1992
Bme18I	GGWCC	3 cut(s)	126, 478, 1988
BmgBI	CACGTC	1 cut(s)	1500
BmgT120I	GGNCC	4 cut(s)	126, 263, 478, 1988
BmiI	GGNNCC	6 cut(s)	22, 128, 152, 265, 744, 1990
BmrFI	CCNGG	1 cut(s)	1992
BmrI	ACTGGG	1 cut(s)	2496
BmsI	GCATC	8 cut(s)	1012, 1136, 1365, 1396, 1403, 1633, 2186, 2577
BmtI	GCTAGC	1 cut(s)	1886
BmuI	ACTGGG	1 cut(s)	2496
BpiI	GAAGAC	1 cut(s)	2238
BplI	GAGNNNNNCTC	2 cut(s)	2091, 2123
BpmI	CTGGAG	2 cut(s)	2226, 2319
Bpu10I	CCTNAGC	1 cut(s)	393
Bpu1102I	GCTNAGC	1 cut(s)	644
BpuEI	CTTGAG	1 cut(s)	2561
BpuMI	CCSGG	1 cut(s)	1992
Bsa29I	ATCGAT	2 cut(s)	312, 1173
BsaBI	GATNNNNATC	1 cut(s)	951
BsaHI	GRCGYC	2 cut(s)	57, 159
BsaJI	CCNNGG	5 cut(s)	154, 180, 994, 1698, 1887
BsaWI	WCCGGW	2 cut(s)	752, 1350
BsaXI	ACNNNNNCTCC	2 cut(s)	2569, 2599
Bsc4I	CCNNNNNNNGG	5 cut(s)	394, 584, 752, 1601, 1985
Bse118I	RCCGGY	1 cut(s)	2182
Bse1I	ACTGG	5 cut(s)	512, 744, 2209, 2502, 2569
Bse21I	CCTNAGG	1 cut(s)	122
Bse8I	GATNNNNATC	1 cut(s)	951
BseAI	TCCGGA	2 cut(s)	752, 1350
BseCI	ATCGAT	2 cut(s)	312, 1173
BseDI	CCNNGG	5 cut(s)	154, 180, 994, 1698, 1887
BseGI	GGATG	7 cut(s)	202, 952, 1027, 1104, 1184, 2201, 2377
BseJI	GATNNNNATC	1 cut(s)	951
BseLI	CCNNNNNNNGG	5 cut(s)	394, 584, 752, 1601, 1985
BseMII	CTCAG	8 cut(s)	136, 230, 407, 658, 966, 1145, 1230, 1481
BseNI	ACTGG	5 cut(s)	512, 744, 2209, 2502, 2569
BseRI	GAGGAG	3 cut(s)	230, 1506, 2558
BseSI	GKGCMC	1 cut(s)	1250
BseXI	GCAGC	2 cut(s)	1702, 1735
BsgI	GTGCAG	1 cut(s)	1734
Bsh1236I	CGCG	5 cut(s)	42, 148, 156, 174, 182
BshFI	GGCC	3 cut(s)	5, 264, 1152
BshNI	GGYRCC	1 cut(s)	150
BshVI	ATCGAT	2 cut(s)	312, 1173
BsiHKAI	GWGCWC	3 cut(s)	169, 645, 2343
BsiSI	CCGG	5 cut(s)	413, 753, 1351, 1992, 2183
BslFI	GGGAC	3 cut(s)	1974, 2484, 2575
BslI	CCNNNNNNNGG	5 cut(s)	394, 584, 752, 1601, 1985
BsmAI	GTCTC	2 cut(s)	1488, 2273
BsmFI	GGGAC	3 cut(s)	1974, 2484, 2575
BsmI	GAATGC	3 cut(s)	14, 546, 614
BsnI	GGCC	3 cut(s)	5, 264, 1152
Bsp1286I	GDGCHC	4 cut(s)	169, 645, 1250, 2343
Bsp13I	TCCGGA	2 cut(s)	752, 1350
Bsp143I	GATC	9 cut(s)	302, 313, 593, 742, 1564, 1786, 2101, 2313, 2480
Bsp1720I	GCTNAGC	1 cut(s)	644
BspANI	GGCC	3 cut(s)	5, 264, 1152
BspCNI	CTCAG	8 cut(s)	135, 231, 406, 657, 967, 1144, 1231, 1480
BspDI	ATCGAT	2 cut(s)	312, 1173
BspEI	TCCGGA	2 cut(s)	752, 1350
BspFNI	CGCG	5 cut(s)	42, 148, 156, 174, 182
BspLI	GGNNCC	6 cut(s)	22, 128, 152, 265, 744, 1990
BspMI	ACCTGC	1 cut(s)	773
BspOI	GCTAGC	1 cut(s)	1886
BspPI	GGATC	4 cut(s)	601, 737, 750, 2488
BspT107I	GGYRCC	1 cut(s)	150
BsrBI	CCGCTC	1 cut(s)	1527
BsrFI	RCCGGY	1 cut(s)	2182
BsrI	ACTGG	5 cut(s)	512, 744, 2209, 2502, 2569
BssAI	RCCGGY	1 cut(s)	2182
BssECI	CCNNGG	5 cut(s)	154, 180, 994, 1698, 1887
BssMI	GATC	9 cut(s)	302, 313, 593, 742, 1564, 1786, 2101, 2313, 2480
BssNI	GRCGYC	2 cut(s)	57, 159
BssSI	CACGAG	1 cut(s)	777
BssT1I	CCWWGG	2 cut(s)	994, 1887
Bst2BI	CACGAG	1 cut(s)	777
Bst4CI	ACNGT	9 cut(s)	205, 667, 714, 805, 1660, 1906, 2267, 2458, 2512
Bst6I	CTCTTC	2 cut(s)	1863, 2092
BstACI	GRCGYC	2 cut(s)	57, 159
BstC8I	GCNNGC	6 cut(s)	7, 1314, 1554, 1586, 1590, 1884
BstDSI	CCRYGG	3 cut(s)	154, 180, 1698
BstF5I	GGATG	7 cut(s)	202, 952, 1027, 1104, 1184, 2201, 2377
BstFNI	CGCG	5 cut(s)	42, 148, 156, 174, 182
BstHHI	GCGC	2 cut(s)	44, 176
BstKTI	GATC	9 cut(s)	305, 316, 596, 745, 1567, 1789, 2104, 2316, 2483
BstMAI	GTCTC	2 cut(s)	1488, 2273
BstMBI	GATC	9 cut(s)	302, 313, 593, 742, 1564, 1786, 2101, 2313, 2480
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 173, 1384, 1549
BstNSI	RCATGY	2 cut(s)	341, 1722
BstSCI	CCNGG	1 cut(s)	1990
BstSLI	GKGCMC	1 cut(s)	1250
BstUI	CGCG	5 cut(s)	42, 148, 156, 174, 182
BstV1I	GCAGC	2 cut(s)	1702, 1735
BstV2I	GAAGAC	1 cut(s)	2238
BstX2I	RGATCY	1 cut(s)	742
BstXI	CCANNNNNNTGG	2 cut(s)	1148, 2133
BstYI	RGATCY	1 cut(s)	742
Bsu15I	ATCGAT	2 cut(s)	312, 1173
Bsu36I	CCTNAGG	1 cut(s)	122
BsuRI	GGCC	3 cut(s)	5, 264, 1152
BsuTUI	ATCGAT	2 cut(s)	312, 1173
BtgI	CCRYGG	3 cut(s)	154, 180, 1698
BtgZI	GCGATG	1 cut(s)	1197
BtrI	CACGTC	1 cut(s)	1500
BtsCI	GGATG	7 cut(s)	202, 952, 1027, 1104, 1184, 2201, 2377
BtsI	GCAGTG	2 cut(s)	1439, 1923
BtsIMutI	CAGTG	7 cut(s)	1260, 1439, 1836, 1923, 2165, 2247, 2508
BveI	ACCTGC	1 cut(s)	773
Cac8I	GCNNGC	6 cut(s)	7, 1314, 1554, 1586, 1590, 1884
CfoI	GCGC	2 cut(s)	44, 176
Cfr10I	RCCGGY	1 cut(s)	2182
Cfr13I	GGNCC	4 cut(s)	126, 263, 478, 1988
Cfr42I	CCGCGG	2 cut(s)	157, 183
ClaI	ATCGAT	2 cut(s)	312, 1173
CseI	GACGC	3 cut(s)	65, 148, 154
Csp6I	GTAC	9 cut(s)	400, 625, 668, 683, 942, 962, 1205, 1617, 1944
CspCI	CAANNNNNGTGG	2 cut(s)	960, 995
CviQI	GTAC	9 cut(s)	400, 625, 668, 683, 942, 962, 1205, 1617, 1944
DpnI	GATC	9 cut(s)	304, 315, 595, 744, 1566, 1788, 2103, 2315, 2482
DpnII	GATC	9 cut(s)	302, 313, 593, 742, 1564, 1786, 2101, 2313, 2480
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	2 cut(s)	1863, 2092
EarI	CTCTTC	2 cut(s)	1863, 2092
EciI	GGCGGA	2 cut(s)	259, 431
Ecl136II	GAGCTC	3 cut(s)	167, 643, 2341
Eco130I	CCWWGG	2 cut(s)	994, 1887
Eco24I	GRGCYC	3 cut(s)	169, 645, 2343
Eco32I	GATATC	1 cut(s)	1834
Eco47I	GGWCC	3 cut(s)	126, 478, 1988
Eco53kI	GAGCTC	3 cut(s)	167, 643, 2341
Eco57I	CTGAAG	3 cut(s)	1934, 1989, 2141
Eco81I	CCTNAGG	1 cut(s)	122
EcoICRI	GAGCTC	3 cut(s)	167, 643, 2341
EcoO109I	RGGNCCY	2 cut(s)	126, 1988
EcoRV	GATATC	1 cut(s)	1834
EcoT14I	CCWWGG	2 cut(s)	994, 1887
EcoT38I	GRGCYC	3 cut(s)	169, 645, 2343
ErhI	CCWWGG	2 cut(s)	994, 1887
FalI	AAGNNNNNCTT	2 cut(s)	1503, 1535
FaqI	GGGAC	3 cut(s)	1974, 2484, 2575
FauNDI	CATATG	1 cut(s)	811
FblI	GTMKAC	1 cut(s)	143
Fnu4HI	GCNGC	8 cut(s)	60, 154, 157, 177, 180, 544, 1716, 1749
FokI	GGATG	7 cut(s)	209, 939, 1034, 1091, 1191, 2208, 2384
FriOI	GRGCYC	3 cut(s)	169, 645, 2343
Fsp4HI	GCNGC	8 cut(s)	60, 154, 157, 177, 180, 544, 1716, 1749
FspBI	CTAG	5 cut(s)	300, 1410, 1422, 1614, 1883
GlaI	GCGC	2 cut(s)	43, 175
GluI	GCNGC	8 cut(s)	60, 154, 157, 177, 180, 544, 1716, 1749
GsuI	CTGGAG	2 cut(s)	2226, 2319
HaeIII	GGCC	3 cut(s)	5, 264, 1152
HapII	CCGG	5 cut(s)	413, 753, 1351, 1992, 2183
HgaI	GACGC	3 cut(s)	65, 148, 154
HhaI	GCGC	2 cut(s)	44, 176
Hin1I	GRCGYC	2 cut(s)	57, 159
Hin6I	GCGC	2 cut(s)	42, 174
HinP1I	GCGC	2 cut(s)	42, 174
HincII	GTYRAC	1 cut(s)	144
HindII	GTYRAC	1 cut(s)	144
HindIII	AAGCTT	4 cut(s)	132, 1145, 1554, 2153
HpaII	CCGG	5 cut(s)	413, 753, 1351, 1992, 2183
HphI	GGTGA	4 cut(s)	545, 1946, 2408, 2537
Hpy166II	GTNNAC	6 cut(s)	144, 400, 499, 1627, 1821, 1946
Hpy8I	GTNNAC	6 cut(s)	144, 400, 499, 1627, 1821, 1946
Hpy99I	CGWCG	5 cut(s)	59, 142, 145, 148, 164
HpyCH4III	ACNGT	9 cut(s)	205, 667, 714, 805, 1660, 1906, 2267, 2458, 2512
HpyCH4IV	ACGT	1 cut(s)	1499
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 173, 1384, 1549
HpySE526I	ACGT	1 cut(s)	1499
Hsp92I	GRCGYC	2 cut(s)	57, 159
HspAI	GCGC	2 cut(s)	42, 174
Kpn2I	TCCGGA	2 cut(s)	752, 1350
KspI	CCGCGG	2 cut(s)	157, 183
Kzo9I	GATC	9 cut(s)	302, 313, 593, 742, 1564, 1786, 2101, 2313, 2480
LmnI	GCTCC	8 cut(s)	164, 172, 640, 1124, 1288, 1353, 2112, 2346
Lsp1109I	GCAGC	2 cut(s)	1702, 1735
LweI	GCATC	8 cut(s)	1012, 1136, 1365, 1396, 1403, 1633, 2186, 2577
MaeI	CTAG	5 cut(s)	300, 1410, 1422, 1614, 1883
MaeII	ACGT	1 cut(s)	1499
MaeIII	GTNAC	4 cut(s)	199, 661, 799, 1157
MalI	GATC	9 cut(s)	304, 315, 595, 744, 1566, 1788, 2103, 2315, 2482
MbiI	CCGCTC	1 cut(s)	1527
MboI	GATC	9 cut(s)	302, 313, 593, 742, 1564, 1786, 2101, 2313, 2480
MboII	GAAGA	8 cut(s)	548, 1021, 1043, 1880, 1949, 2106, 2109, 2243
MfeI	CAATTG	1 cut(s)	918
MflI	RGATCY	1 cut(s)	742
MhlI	GDGCHC	4 cut(s)	169, 645, 1250, 2343
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	252, 353, 2506
MmeI	TCCRAC	6 cut(s)	142, 171, 540, 696, 1079, 1941
Mox20I	TGGCCA	1 cut(s)	5
MroI	TCCGGA	2 cut(s)	752, 1350
MroXI	GAANNNNTTC	4 cut(s)	865, 888, 1575, 1974
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	8 cut(s)	1269, 1362, 1446, 1854, 2046, 2112, 2178, 2632
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	156, 182
MspI	CCGG	5 cut(s)	413, 753, 1351, 1992, 2183
MspR9I	CCNGG	1 cut(s)	1992
MunI	CAATTG	1 cut(s)	918
Mva1269I	GAATGC	3 cut(s)	14, 546, 614
MvnI	CGCG	5 cut(s)	42, 148, 156, 174, 182
MwoI	GCNNNNNNNGC	4 cut(s)	11, 173, 1384, 1549
NciI	CCSGG	1 cut(s)	1992
NdeI	CATATG	1 cut(s)	811
NdeII	GATC	9 cut(s)	302, 313, 593, 742, 1564, 1786, 2101, 2313, 2480
NheI	GCTAGC	1 cut(s)	1882
NlaIV	GGNNCC	6 cut(s)	22, 128, 152, 265, 744, 1990
NmeAIII	GCCGAG	1 cut(s)	298
NmuCI	GTSAC	1 cut(s)	1157
NspI	RCATGY	2 cut(s)	341, 1722
PciI	ACATGT	1 cut(s)	337
PcsI	WCGNNNNNNNCGW	1 cut(s)	2313
PctI	GAATGC	3 cut(s)	14, 546, 614
PdmI	GAANNNNTTC	4 cut(s)	865, 888, 1575, 1974
PflFI	GACNNNGTC	1 cut(s)	2024
PflMI	CCANNNNNTGG	1 cut(s)	584
PkrI	GCNGC	8 cut(s)	61, 155, 158, 178, 181, 545, 1717, 1750
PleI	GAGTC	3 cut(s)	251, 353, 2505
PpsI	GAGTC	3 cut(s)	251, 353, 2505
PpuMI	RGGWCCY	2 cut(s)	126, 1988
PscI	ACATGT	1 cut(s)	337
PshBI	ATTAAT	1 cut(s)	1362
Psp124BI	GAGCTC	3 cut(s)	169, 645, 2343
Psp5II	RGGWCCY	2 cut(s)	126, 1988
PspN4I	GGNNCC	6 cut(s)	22, 128, 152, 265, 744, 1990
PspPI	GGNCC	4 cut(s)	126, 263, 478, 1988
PspPPI	RGGWCCY	2 cut(s)	126, 1988
PsuI	RGATCY	1 cut(s)	742
PsyI	GACNNNGTC	1 cut(s)	2024
RsaI	GTAC	9 cut(s)	401, 626, 669, 684, 943, 963, 1206, 1618, 1945
RsaNI	GTAC	9 cut(s)	400, 625, 668, 683, 942, 962, 1205, 1617, 1944
SacI	GAGCTC	3 cut(s)	169, 645, 2343
SacII	CCGCGG	2 cut(s)	157, 183
SalI	GTCGAC	1 cut(s)	142
SaqAI	TTAA	8 cut(s)	1269, 1362, 1446, 1854, 2046, 2112, 2178, 2632
SatI	GCNGC	8 cut(s)	60, 154, 157, 177, 180, 544, 1716, 1749
Sau3AI	GATC	9 cut(s)	302, 313, 593, 742, 1564, 1786, 2101, 2313, 2480
Sau96I	GGNCC	4 cut(s)	126, 263, 478, 1988
ScaI	AGTACT	1 cut(s)	1618
SchI	GAGTC	3 cut(s)	252, 353, 2506
ScrFI	CCNGG	1 cut(s)	1992
SduI	GDGCHC	4 cut(s)	169, 645, 1250, 2343
SfaNI	GCATC	8 cut(s)	1012, 1136, 1365, 1396, 1403, 1633, 2186, 2577
Sfr303I	CCGCGG	2 cut(s)	157, 183
SgrBI	CCGCGG	2 cut(s)	157, 183
SgrDI	CGTCGACG	1 cut(s)	142
SinI	GGWCC	3 cut(s)	126, 478, 1988
SmlI	CTYRAG	1 cut(s)	2540
SmoI	CTYRAG	1 cut(s)	2540
SspI	AATATT	4 cut(s)	888, 1114, 1254, 1345
SspMI	CTAG	5 cut(s)	300, 1410, 1422, 1614, 1883
SstI	GAGCTC	3 cut(s)	169, 645, 2343
StyD4I	CCNGG	1 cut(s)	1990
StyI	CCWWGG	2 cut(s)	994, 1887
TaaI	ACNGT	9 cut(s)	205, 667, 714, 805, 1660, 1906, 2267, 2458, 2512
TaiI	ACGT	1 cut(s)	1502
TaqI	TCGA	9 cut(s)	143, 207, 312, 435, 618, 1117, 1168, 1173, 2316
TaqII	GACCGA	1 cut(s)	1758
TatI	WGTACW	5 cut(s)	399, 624, 682, 961, 1616
TauI	GCSGC	6 cut(s)	62, 156, 159, 179, 182, 546
Tru1I	TTAA	8 cut(s)	1269, 1362, 1446, 1854, 2046, 2112, 2178, 2632
Tru9I	TTAA	8 cut(s)	1269, 1362, 1446, 1854, 2046, 2112, 2178, 2632
TscAI	CASTG	7 cut(s)	1267, 1446, 1843, 1923, 2165, 2247, 2515
TseFI	GTSAC	1 cut(s)	1157
TseI	GCWGC	2 cut(s)	1715, 1748
Tsp45I	GTSAC	1 cut(s)	1157
TspDTI	ATGAA	8 cut(s)	717, 1190, 1650, 1857, 2154, 2244, 2376, 2418
TspGWI	ACGGA	5 cut(s)	1715, 1783, 2322, 2384, 2577
TspRI	CASTG	7 cut(s)	1267, 1446, 1843, 1923, 2165, 2247, 2515
Tth111I	GACNNNGTC	1 cut(s)	2024
Van91I	CCANNNNNTGG	1 cut(s)	584
VpaK11BI	GGWCC	3 cut(s)	126, 478, 1988
VspI	ATTAAT	1 cut(s)	1362
XapI	RAATTY	3 cut(s)	908, 1856, 2549
XceI	RCATGY	2 cut(s)	341, 1722
XmiI	GTMKAC	1 cut(s)	143
XmnI	GAANNNNTTC	4 cut(s)	865, 888, 1575, 1974
XspI	CTAG	5 cut(s)	300, 1410, 1422, 1614, 1883
ZrmI	AGTACT	1 cut(s)	1618

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.