Rroxscaffold_6G00418850

D-ala D-ala ligase N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005073
Physical Location & Seq
Forward (+)
40404700 .. 40412777
8078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418850.1

Sequence Viewer

Length: 2769 bp
ATGCTCTTCTTCACCACTCCTACTCCTCCACCTCACTCTCTCCCTCTTTTTCTCGTCGCCGATCATTTCCCGATGGCCAGCCTTGCATTTGGTTCCAGCCTCACTTTACTTCGCGCCAACCAAAACGACGCCGCACAGAATAAGCCTCTTATCTACTCTCCCAATTTCAACTCTCTCCGATTCAACCACAGCCTCAGAACCCACGCTTCGTCGTCGACGCGGTGCCGCGGCGTCCAAGCTCCACGCGCCGCCGCGGAGGTGGTGGAGGATGTAACTGTCGAGGGAGAGGAGGAGGAGGAGGAGAATCGTAGGGTTCTGAGAGTCGGGGTCATCTGCGGTGGCCCCTCCGCCGAGCGTGGAATCTCTCTCAACTCCGCTAGATCAGTAATCGATCATATTCAAGGAGAGGATTTACATGTGAGTTGCTACTACATTGACTCTGAACTCAACGCTTTTGCAATATCCCCTGCTCAGGTGTACTCCAACACTCCGGCGGATTTTGATTTCAAACTTGAAAGCCTTGCTCAAGGTTTCAAGTCTTGGTCTGACTTTGCGGACCACCTTGCTGTCAATGTGGACATAGTGTTTCCAGTCATACATGGTCAATTTGGTGAAGATGGCGGCATTCAGGAGGTGTTGGAAAGGTATAACATTCCATTTGTTGGCACGGGATCAAATGAGTGTCGTCAAGCATTCGACAAGTACAATGCCTCATTGGAGCTCAGCAGACATGGATTCGTAACTGTACCAAGTTGTTTAGTACAGGGAAGTAAAGCAGATGAACCTGAACTGTCAGAATGGTTTGCAAAAAATCAACTGGATCCTAAGTCCGGAAAAGTTGTGGTAAAACCAGCACGAGCAGGTTCAAGCATTGGTGTTACAGTTGCATATGGCTTGGCTGATTCTCTTAGCAAGGCTAATGCAATTATTACAGAGGGAATTGATTCCAAAGTCCTTGTTGAAATATTTCTTGAAGGAGGAGTGAATTTACAGCAATTGTTCTTGATGTGGGGTATGGTGGAACTTCAATTCCTTGGTAGTGTAGATGTCAGAGAGAAGGATGCAATCTTCAACTACCGCAGGAAATATCTTCCAACACAACAGGTTGCTTATCATACTCCACCTCGTTTTCCTATTGATGTCATCCAAAATATTCGAGATGGAGCATCTCAGTTATTCCAAAAGCTTGGCCTTCGTGACTTTGCTCGAATCGATGGATGGTTTCTGCCTAATTCTATTCATGTACCATCATCGCCAGATAGCAAGTTTGGAAGGACTGAGATGGGCACAATATTATACACTGACATTAACCTGATTAGTGGTATGGAGCAGACCAGCTTTTTATTCCAGCAAGCCTCAAAGGTTGGGTTTTCTCATGCAAATATTCTCCGGAGCATCATTAATCATGCTTGTTTGCGATTTCCGCATCTTGCATCTTGTGATGTGTCTAGTGATTTGCCTAGAACATCAAAATCCCCACTGCTTAAAGATGATTGGGAAGGCACTAAGAAAGTTTTTGTTATTTTTGGAGGAGACACGTCAGAGAGGCAAGTATCTCTTATGAGTGGAACAAATGTTTGGCTCAATTTGCAAGCTTTTGATGATCTGGAAGTGCTTCCTTGCTTGCTTGCCCCCACGAATGGATATTCGTCTAGTACTGATGTGGACAAAAATGAAGTTGATGCAACTTCCAAAACAGTTTGGTCATTACCTTATTCTCTTGTGCTAAGACACACCACGGAGGAAGTTCTTGCTGCATGTGTAGAGGCAATAGAACCAGACCGAGCTGCACTTACATCTCAATTACGGAACAGAGTTATTGATGATCTTATGGAAGGCTTCAAGAAACATTCTTGGTTTACAGGATTTGATATCACTGATGAACTGCCTGTTAAATTTTCTATTGAAGAGTGGATAAAGCTAGCCAAGGAAGTCAAGGCAACAGTATTTATTGCAGTGCATGGAGGCATTGGTGAAGATGGTACACTTCAGTCTTGGTTGGAGGCTGAAGGGATTCCACATACAGGTCCCGGTGTTTTGGCTTCAAAGATTTGTATGGACAAAGTCGCAACATCAGTTGCTCTTAAACATCTATCGGACTTGGGAGTTCTTACCATAAACAAAGATTTGAGAAGAAGAGATGAGCTCCTTAATACACCAATACCAAATGTATGGCATGAATTGACCTCCAAGCTTCAGTGTGAAACATTATGTGTTAAACCGGCAAGAGATGGATGCTCAACTGGAGTTGCAAGGTTATGCTGTGATGAAGACCTTTCAGTGTATGTAAAAGCATTGGAGGACTGTCTCCTTCGGATTCCCCCAAATAGTTTGTCAAAGAGTAGATGGGTTGAAATAACTGTTGGTGTTATTGGGAAACAAGGATCAATGCACTCATTGAGTCCCAGTATCACCGTGAAGGAGAGTGGTGATATTTTATCACTTGAGGAGAAATTTCAGGGTGGGACTGGCATCAATCTTACTCCACCTCCGTCATCGATCATTAGCCACGAGGCCTTGCAGAAATGTAAACAACACATTGAAATAATTGCCAACACTTTAGAATTAGAAGGATTTTCACGAATTGATGCCTTTGTCAACGTGGATAGTGGAGAGGTGCTGGTTATAGAGGTCAATACAGTGCCGGGAATGACACCTTCCACTGTTCTGATTCACCAGGCACTAGCAGAGGAACCGCCCATGTATCCTCACCAGTTCTTCCGGACACTGCTTGATTTGGCATCAGAGAGGACAATATACGTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dala_Dala_lig_C Dala_Dala_lig_N

Protein Analysis

922

Amino Acids

101.55

Weight (kDa)

5.24

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dala_Dala_lig_N PF01820 107 - 222 2.1e-24 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 280 - 405 1.2e-13 D-ala D-ala ligase C-terminus
Dala_Dala_lig_N PF01820 504 - 677 5.4e-17 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 737 - 890 8.8e-16 D-ala D-ala ligase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 851
AccB1I GGYRCC 1 cut(s) 222
AccB7I CCANNNNNTGG 1 cut(s) 662
AccI GTMKAC 1 cut(s) 215
AccII CGCG 5 cut(s) 114, 220, 228, 246, 254
AccIII TCCGGA 3 cut(s) 830, 1389, 2721
AclWI GGATC 4 cut(s) 679, 815, 828, 2392
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 3 cut(s) 985, 1895, 2453
AcuI CTGAAG 3 cut(s) 1973, 2028, 2180
AcyI GRCGYC 2 cut(s) 129, 231
AfaI GTAC 7 cut(s) 479, 704, 747, 762, 1245, 1657, 1984
AfiI CCNNNNNNNGG 5 cut(s) 472, 662, 830, 1640, 2024
AflIII ACRYGT 2 cut(s) 415, 1536
AjiI CACGTC 1 cut(s) 1539
AjnI CCWGG 1 cut(s) 2676
AjuI GAANNNNNNNTTGG 4 cut(s) 1561, 1593, 2346, 2378
AloI GAACNNNNNNTCC 2 cut(s) 1014, 1046
AluBI AGCT 9 cut(s) 239, 721, 1186, 1338, 1595, 1787, 1921, 2146, 2194
AluI AGCT 9 cut(s) 239, 721, 1186, 1338, 1595, 1787, 1921, 2146, 2194
Alw21I GWGCWC 2 cut(s) 723, 2148
Alw26I GTCTC 2 cut(s) 1527, 2312
AlwI GGATC 4 cut(s) 679, 815, 828, 2392
Aor13HI TCCGGA 3 cut(s) 830, 1389, 2721
AoxI GGCC 4 cut(s) 75, 340, 1189, 2514
ApeKI GCWGC 2 cut(s) 1754, 1787
ApoI RAATTY 3 cut(s) 985, 1895, 2453
ArsI GACNNNNNNTTYG 2 cut(s) 2318, 2350
AseI ATTAAT 1 cut(s) 1401
Asp700I GAANNNNTTC 4 cut(s) 943, 966, 1614, 2013
AspLEI GCGC 2 cut(s) 116, 248
AspS9I GGNCC 3 cut(s) 341, 556, 2027
AsuC2I CCSGG 2 cut(s) 2031, 2646
AsuHPI GGTGA 7 cut(s) 4, 623, 1985, 2404, 2441, 2666, 2702
AsuNHI GCTAGC 1 cut(s) 1921
AvaII GGWCC 2 cut(s) 556, 2027
BaeGI GKGCMC 1 cut(s) 1289
BalI TGGCCA 1 cut(s) 77
BamHI GGATCC 1 cut(s) 820
BanI GGYRCC 1 cut(s) 222
BanII GRGCYC 2 cut(s) 723, 2148
BauI CACGAG 2 cut(s) 855, 2510
BbsI GAAGAC 1 cut(s) 2277
Bbv12I GWGCWC 2 cut(s) 723, 2148
BbvI GCAGC 2 cut(s) 1741, 1774
BciT130I CCWGG 1 cut(s) 2678
BciVI GTATCC 1 cut(s) 2715
BcnI CCSGG 2 cut(s) 2031, 2646
BcoDI GTCTC 2 cut(s) 1527, 2312
BfaI CTAG 6 cut(s) 378, 1449, 1461, 1653, 1922, 2684
BfuAI ACCTGC 1 cut(s) 851
BfuI GTATCC 1 cut(s) 2715
BisI GCNGC 8 cut(s) 132, 226, 229, 249, 252, 622, 1755, 1788
BlpI GCTNAGC 1 cut(s) 722
BlsI GCNGC 8 cut(s) 133, 227, 230, 250, 253, 623, 1756, 1789
BmcAI AGTACT 1 cut(s) 1657
Bme1390I CCNGG 3 cut(s) 2031, 2646, 2678
Bme18I GGWCC 2 cut(s) 556, 2027
BmgBI CACGTC 1 cut(s) 1539
BmgT120I GGNCC 3 cut(s) 341, 556, 2027
BmiI GGNNCC 6 cut(s) 94, 224, 343, 822, 2029, 2694
BmrFI CCNGG 3 cut(s) 2031, 2646, 2678
BmrI ACTGGG 1 cut(s) 2400
BmtI GCTAGC 1 cut(s) 1925
BmuI ACTGGG 1 cut(s) 2400
BpiI GAAGAC 1 cut(s) 2277
BplI GAGNNNNNCTC 2 cut(s) 2130, 2162
BpmI CTGGAG 1 cut(s) 2265
Bpu10I CCTNAGC 1 cut(s) 471
Bpu1102I GCTNAGC 1 cut(s) 722
BpuEI CTTGAG 2 cut(s) 510, 2465
BpuMI CCSGG 2 cut(s) 2031, 2646
Bsa29I ATCGAT 3 cut(s) 390, 1212, 2498
BsaHI GRCGYC 2 cut(s) 129, 231
BsaJI CCNNGG 5 cut(s) 226, 252, 1033, 1737, 1926
BsaWI WCCGGW 3 cut(s) 830, 1389, 2721
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 2473, 2503
Bsc4I CCNNNNNNNGG 5 cut(s) 472, 662, 830, 1640, 2024
Bse118I RCCGGY 1 cut(s) 2221
Bse1I ACTGG 6 cut(s) 590, 822, 2248, 2406, 2473, 2713
BseAI TCCGGA 3 cut(s) 830, 1389, 2721
BseBI CCWGG 1 cut(s) 2678
BseCI ATCGAT 3 cut(s) 390, 1212, 2498
BseDI CCNNGG 5 cut(s) 226, 252, 1033, 1737, 1926
BseGI GGATG 5 cut(s) 274, 1066, 1143, 1223, 2240
BseLI CCNNNNNNNGG 5 cut(s) 472, 662, 830, 1640, 2024
BseMII CTCAG 6 cut(s) 208, 308, 485, 736, 1184, 1269
BseNI ACTGG 6 cut(s) 590, 822, 2248, 2406, 2473, 2713
BseRI GAGGAG 9 cut(s) 15, 302, 305, 308, 311, 314, 993, 1545, 2462
BseSI GKGCMC 1 cut(s) 1289
BseXI GCAGC 2 cut(s) 1741, 1774
BsgI GTGCAG 1 cut(s) 1773
Bsh1236I CGCG 5 cut(s) 114, 220, 228, 246, 254
BshFI GGCC 4 cut(s) 77, 342, 1191, 2516
BshNI GGYRCC 1 cut(s) 222
BshVI ATCGAT 3 cut(s) 390, 1212, 2498
BsiHKAI GWGCWC 2 cut(s) 723, 2148
BsiSI CCGG 7 cut(s) 491, 831, 1390, 2031, 2222, 2645, 2722
BslFI GGGAC 3 cut(s) 2013, 2388, 2479
BslI CCNNNNNNNGG 5 cut(s) 472, 662, 830, 1640, 2024
BsmAI GTCTC 2 cut(s) 1527, 2312
BsmFI GGGAC 3 cut(s) 2013, 2388, 2479
BsmI GAATGC 2 cut(s) 624, 692
BsnI GGCC 4 cut(s) 77, 342, 1191, 2516
Bsp1286I GDGCHC 3 cut(s) 723, 1289, 2148
Bsp13I TCCGGA 3 cut(s) 830, 1389, 2721
Bsp143I GATC 9 cut(s) 61, 380, 391, 671, 820, 1603, 1825, 2384, 2499
Bsp1720I GCTNAGC 1 cut(s) 722
BspANI GGCC 4 cut(s) 77, 342, 1191, 2516
BspCNI CTCAG 6 cut(s) 207, 309, 484, 735, 1183, 1270
BspDI ATCGAT 3 cut(s) 390, 1212, 2498
BspEI TCCGGA 3 cut(s) 830, 1389, 2721
BspFNI CGCG 5 cut(s) 114, 220, 228, 246, 254
BspLI GGNNCC 6 cut(s) 94, 224, 343, 822, 2029, 2694
BspMI ACCTGC 1 cut(s) 851
BspOI GCTAGC 1 cut(s) 1925
BspPI GGATC 4 cut(s) 679, 815, 828, 2392
BspQI GCTCTTC 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 2221
BsrI ACTGG 6 cut(s) 590, 822, 2248, 2406, 2473, 2713
BssAI RCCGGY 1 cut(s) 2221
BssECI CCNNGG 5 cut(s) 226, 252, 1033, 1737, 1926
BssMI GATC 9 cut(s) 61, 380, 391, 671, 820, 1603, 1825, 2384, 2499
BssNI GRCGYC 2 cut(s) 129, 231
BssSI CACGAG 2 cut(s) 855, 2510
BssT1I CCWWGG 2 cut(s) 1033, 1926
Bst2BI CACGAG 2 cut(s) 855, 2510
Bst2UI CCWGG 1 cut(s) 2678
Bst6I CTCTTC 3 cut(s) 11, 1902, 2131
BstACI GRCGYC 2 cut(s) 129, 231
BstC8I GCNNGC 6 cut(s) 79, 1353, 1593, 1625, 1629, 1923
BstDSI CCRYGG 3 cut(s) 226, 252, 1737
BstF5I GGATG 5 cut(s) 274, 1066, 1143, 1223, 2240
BstFNI CGCG 5 cut(s) 114, 220, 228, 246, 254
BstHHI GCGC 2 cut(s) 116, 248
BstKTI GATC 9 cut(s) 64, 383, 394, 674, 823, 1606, 1828, 2387, 2502
BstMAI GTCTC 2 cut(s) 1527, 2312
BstMBI GATC 9 cut(s) 61, 380, 391, 671, 820, 1603, 1825, 2384, 2499
BstMWI GCNNNNNNNGC 4 cut(s) 83, 245, 1423, 1588
BstNI CCWGG 1 cut(s) 2678
BstNSI RCATGY 2 cut(s) 419, 1761
BstSCI CCNGG 3 cut(s) 2029, 2644, 2676
BstSLI GKGCMC 1 cut(s) 1289
BstUI CGCG 5 cut(s) 114, 220, 228, 246, 254
BstV1I GCAGC 2 cut(s) 1741, 1774
BstV2I GAAGAC 1 cut(s) 2277
BstX2I RGATCY 1 cut(s) 820
BstXI CCANNNNNNTGG 2 cut(s) 1187, 2172
BstYI RGATCY 1 cut(s) 820
Bsu15I ATCGAT 3 cut(s) 390, 1212, 2498
BsuI GTATCC 1 cut(s) 2715
BsuRI GGCC 4 cut(s) 77, 342, 1191, 2516
BsuTUI ATCGAT 3 cut(s) 390, 1212, 2498
BtgI CCRYGG 3 cut(s) 226, 252, 1737
BtgZI GCGATG 1 cut(s) 1236
BtrI CACGTC 1 cut(s) 1539
BtsCI GGATG 5 cut(s) 274, 1066, 1143, 1223, 2240
BtsI GCAGTG 3 cut(s) 1478, 1962, 2726
BtsIMutI CAGTG 9 cut(s) 1299, 1478, 1875, 1962, 2204, 2286, 2646, 2661, 2726
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 6 cut(s) 79, 1353, 1593, 1625, 1629, 1923
CfoI GCGC 2 cut(s) 116, 248
Cfr10I RCCGGY 1 cut(s) 2221
Cfr13I GGNCC 3 cut(s) 341, 556, 2027
Cfr42I CCGCGG 2 cut(s) 229, 255
ClaI ATCGAT 3 cut(s) 390, 1212, 2498
CseI GACGC 3 cut(s) 137, 220, 226
Csp6I GTAC 7 cut(s) 478, 703, 746, 761, 1244, 1656, 1983
CviQI GTAC 7 cut(s) 478, 703, 746, 761, 1244, 1656, 1983
DpnI GATC 9 cut(s) 63, 382, 393, 673, 822, 1605, 1827, 2386, 2501
DpnII GATC 9 cut(s) 61, 380, 391, 671, 820, 1603, 1825, 2384, 2499
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 3 cut(s) 11, 1902, 2131
EarI CTCTTC 3 cut(s) 11, 1902, 2131
EciI GGCGGA 2 cut(s) 337, 509
Ecl136II GAGCTC 2 cut(s) 721, 2146
Eco130I CCWWGG 2 cut(s) 1033, 1926
Eco147I AGGCCT 1 cut(s) 2516
Eco24I GRGCYC 2 cut(s) 723, 2148
Eco32I GATATC 1 cut(s) 1873
Eco47I GGWCC 2 cut(s) 556, 2027
Eco53kI GAGCTC 2 cut(s) 721, 2146
Eco57I CTGAAG 3 cut(s) 1973, 2028, 2180
EcoICRI GAGCTC 2 cut(s) 721, 2146
EcoO109I RGGNCCY 1 cut(s) 2027
EcoRII CCWGG 1 cut(s) 2676
EcoRV GATATC 1 cut(s) 1873
EcoT14I CCWWGG 2 cut(s) 1033, 1926
EcoT38I GRGCYC 2 cut(s) 723, 2148
ErhI CCWWGG 2 cut(s) 1033, 1926
FalI AAGNNNNNCTT 2 cut(s) 1542, 1574
FaqI GGGAC 3 cut(s) 2013, 2388, 2479
FauNDI CATATG 1 cut(s) 889
FblI GTMKAC 1 cut(s) 215
Fnu4HI GCNGC 8 cut(s) 132, 226, 229, 249, 252, 622, 1755, 1788
FokI GGATG 5 cut(s) 281, 1073, 1130, 1230, 2247
FriOI GRGCYC 2 cut(s) 723, 2148
Fsp4HI GCNGC 8 cut(s) 132, 226, 229, 249, 252, 622, 1755, 1788
FspBI CTAG 6 cut(s) 378, 1449, 1461, 1653, 1922, 2684
GlaI GCGC 2 cut(s) 115, 247
GluI GCNGC 8 cut(s) 132, 226, 229, 249, 252, 622, 1755, 1788
GsuI CTGGAG 1 cut(s) 2265
HaeIII GGCC 4 cut(s) 77, 342, 1191, 2516
HapII CCGG 7 cut(s) 491, 831, 1390, 2031, 2222, 2645, 2722
HgaI GACGC 3 cut(s) 137, 220, 226
HhaI GCGC 2 cut(s) 116, 248
Hin1I GRCGYC 2 cut(s) 129, 231
Hin6I GCGC 2 cut(s) 114, 246
HinP1I GCGC 2 cut(s) 114, 246
HincII GTYRAC 2 cut(s) 216, 2599
HindII GTYRAC 2 cut(s) 216, 2599
HindIII AAGCTT 3 cut(s) 1184, 1593, 2192
HpaII CCGG 7 cut(s) 491, 831, 1390, 2031, 2222, 2645, 2722
HphI GGTGA 7 cut(s) 4, 623, 1985, 2404, 2441, 2666, 2702
Hpy166II GTNNAC 8 cut(s) 216, 478, 577, 1666, 1860, 1985, 2531, 2599
Hpy8I GTNNAC 8 cut(s) 216, 478, 577, 1666, 1860, 1985, 2531, 2599
Hpy99I CGWCG 5 cut(s) 59, 131, 214, 217, 220
HpyCH4IV ACGT 3 cut(s) 1538, 2601, 2760
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 245, 1423, 1588
HpySE526I ACGT 3 cut(s) 1538, 2601, 2760
Hsp92I GRCGYC 2 cut(s) 129, 231
HspAI GCGC 2 cut(s) 114, 246
Kpn2I TCCGGA 3 cut(s) 830, 1389, 2721
KspI CCGCGG 2 cut(s) 229, 255
Kzo9I GATC 9 cut(s) 61, 380, 391, 671, 820, 1603, 1825, 2384, 2499
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 6 cut(s) 244, 718, 1163, 1327, 1392, 2151
Lsp1109I GCAGC 2 cut(s) 1741, 1774
MaeI CTAG 6 cut(s) 378, 1449, 1461, 1653, 1922, 2684
MaeII ACGT 3 cut(s) 1538, 2601, 2760
MaeIII GTNAC 4 cut(s) 271, 739, 877, 1196
MalI GATC 9 cut(s) 63, 382, 393, 673, 822, 1605, 1827, 2386, 2501
MboI GATC 9 cut(s) 61, 380, 391, 671, 820, 1603, 1825, 2384, 2499
MboII GAAGA 9 cut(s) 626, 1060, 1082, 1919, 1988, 2145, 2148, 2282, 2710
MfeI CAATTG 1 cut(s) 995
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 3 cut(s) 723, 1289, 2148
MlsI TGGCCA 1 cut(s) 77
MluNI TGGCCA 1 cut(s) 77
MlyI GAGTC 3 cut(s) 330, 431, 2410
MmeI TCCRAC 4 cut(s) 507, 618, 1118, 1980
Mox20I TGGCCA 1 cut(s) 77
MroI TCCGGA 3 cut(s) 830, 1389, 2721
MroXI GAANNNNTTC 4 cut(s) 943, 966, 1614, 2013
MscI TGGCCA 1 cut(s) 77
MseI TTAA 7 cut(s) 1308, 1401, 1485, 1893, 2085, 2151, 2217
Msp20I TGGCCA 1 cut(s) 77
MspA1I CMGCKG 2 cut(s) 228, 254
MspI CCGG 7 cut(s) 491, 831, 1390, 2031, 2222, 2645, 2722
MspR9I CCNGG 3 cut(s) 2031, 2646, 2678
MunI CAATTG 1 cut(s) 995
Mva1269I GAATGC 2 cut(s) 624, 692
MvaI CCWGG 1 cut(s) 2678
MvnI CGCG 5 cut(s) 114, 220, 228, 246, 254
MwoI GCNNNNNNNGC 4 cut(s) 83, 245, 1423, 1588
NciI CCSGG 2 cut(s) 2031, 2646
NdeI CATATG 1 cut(s) 889
NdeII GATC 9 cut(s) 61, 380, 391, 671, 820, 1603, 1825, 2384, 2499
NheI GCTAGC 1 cut(s) 1921
NlaIV GGNNCC 6 cut(s) 94, 224, 343, 822, 2029, 2694
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 1196
NspI RCATGY 2 cut(s) 419, 1761
PceI AGGCCT 1 cut(s) 2516
PciI ACATGT 1 cut(s) 415
PciSI GCTCTTC 1 cut(s) 11
PctI GAATGC 2 cut(s) 624, 692
PdmI GAANNNNTTC 4 cut(s) 943, 966, 1614, 2013
PflFI GACNNNGTC 1 cut(s) 2063
PflMI CCANNNNNTGG 1 cut(s) 662
PkrI GCNGC 8 cut(s) 133, 227, 230, 250, 253, 623, 1756, 1789
PleI GAGTC 3 cut(s) 329, 431, 2409
PpsI GAGTC 3 cut(s) 329, 431, 2409
PpuMI RGGWCCY 1 cut(s) 2027
PscI ACATGT 1 cut(s) 415
PshBI ATTAAT 1 cut(s) 1401
Psp124BI GAGCTC 2 cut(s) 723, 2148
Psp5II RGGWCCY 1 cut(s) 2027
Psp6I CCWGG 1 cut(s) 2676
PspGI CCWGG 1 cut(s) 2676
PspN4I GGNNCC 6 cut(s) 94, 224, 343, 822, 2029, 2694
PspPI GGNCC 3 cut(s) 341, 556, 2027
PspPPI RGGWCCY 1 cut(s) 2027
PsuI RGATCY 1 cut(s) 820
PsyI GACNNNGTC 1 cut(s) 2063
RsaI GTAC 7 cut(s) 479, 704, 747, 762, 1245, 1657, 1984
RsaNI GTAC 7 cut(s) 478, 703, 746, 761, 1244, 1656, 1983
SacI GAGCTC 2 cut(s) 723, 2148
SacII CCGCGG 2 cut(s) 229, 255
SalI GTCGAC 1 cut(s) 214
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 7 cut(s) 1308, 1401, 1485, 1893, 2085, 2151, 2217
SatI GCNGC 8 cut(s) 132, 226, 229, 249, 252, 622, 1755, 1788
Sau3AI GATC 9 cut(s) 61, 380, 391, 671, 820, 1603, 1825, 2384, 2499
Sau96I GGNCC 3 cut(s) 341, 556, 2027
ScaI AGTACT 1 cut(s) 1657
SchI GAGTC 3 cut(s) 330, 431, 2410
ScrFI CCNGG 3 cut(s) 2031, 2646, 2678
SduI GDGCHC 3 cut(s) 723, 1289, 2148
Sfr303I CCGCGG 2 cut(s) 229, 255
SgrBI CCGCGG 2 cut(s) 229, 255
SgrDI CGTCGACG 1 cut(s) 214
SinI GGWCC 2 cut(s) 556, 2027
SmlI CTYRAG 2 cut(s) 525, 2444
SmoI CTYRAG 2 cut(s) 525, 2444
SseBI AGGCCT 1 cut(s) 2516
SspI AATATT 4 cut(s) 966, 1153, 1293, 1384
SspMI CTAG 6 cut(s) 378, 1449, 1461, 1653, 1922, 2684
SstI GAGCTC 2 cut(s) 723, 2148
StuI AGGCCT 1 cut(s) 2516
StyD4I CCNGG 3 cut(s) 2029, 2644, 2676
StyI CCWWGG 2 cut(s) 1033, 1926
TaiI ACGT 3 cut(s) 1541, 2604, 2763
TaqI TCGA 8 cut(s) 215, 279, 390, 696, 1156, 1207, 1212, 2498
TaqII GACCGA 1 cut(s) 1797
TatI WGTACW 4 cut(s) 477, 702, 760, 1655
TauI GCSGC 6 cut(s) 134, 228, 231, 251, 254, 624
Tru1I TTAA 7 cut(s) 1308, 1401, 1485, 1893, 2085, 2151, 2217
Tru9I TTAA 7 cut(s) 1308, 1401, 1485, 1893, 2085, 2151, 2217
TscAI CASTG 9 cut(s) 1306, 1485, 1882, 1962, 2204, 2286, 2646, 2668, 2733
TseFI GTSAC 1 cut(s) 1196
TseI GCWGC 2 cut(s) 1754, 1787
Tsp45I GTSAC 1 cut(s) 1196
TspDTI ATGAA 6 cut(s) 795, 1229, 1689, 1896, 2193, 2283
TspGWI ACGGA 3 cut(s) 1754, 1822, 2481
TspRI CASTG 9 cut(s) 1306, 1485, 1882, 1962, 2204, 2286, 2646, 2668, 2733
Tth111I GACNNNGTC 1 cut(s) 2063
Van91I CCANNNNNTGG 1 cut(s) 662
VpaK11BI GGWCC 2 cut(s) 556, 2027
VspI ATTAAT 1 cut(s) 1401
XapI RAATTY 3 cut(s) 985, 1895, 2453
XceI RCATGY 2 cut(s) 419, 1761
XmiI GTMKAC 1 cut(s) 215
XmnI GAANNNNTTC 4 cut(s) 943, 966, 1614, 2013
XspI CTAG 6 cut(s) 378, 1449, 1461, 1653, 1922, 2684
ZrmI AGTACT 1 cut(s) 1657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.