MD12G1182400.v1.1

D-ala D-ala ligase N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005073
Physical Location & Seq
Reverse (-)
26210910 .. 26218815
7906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1182400.v1.1.491

Sequence Viewer

Length: 2838 bp
ATGGCGACGATCGCATTTGGTTCCGGCCTCGTTTCAGCTCGCGCCGAGCAAAGCGACGGCGTTCGGAACTCGGTGCTGTACTCCCCGAACTTCAATTCCTTGCGGTTGAGGAGAAGTTGTGCTTCGACGACGCGGTGGCGCGGGGTCGGAGTCACACGCGCCACCGCGGAGGCGGTGGAAGGTCGAGTTATTGAGACGGAAGGGGAGGTGAAAGAGAAGGAGAAATGTAGGGTTCTTAGAGTTGGCGTCGTTTGCGGAGGTCCCTCGGCGGAGCGTGGGATTTCTCTCAACTCGGCTCGATCAGTTCTTGATCACATTCAGGGAGGTGATATACATGTGAGTTGCTATTACGTTGACGCTGGCCTTAATGCGTTTGCAATATCCCCTGCTCAGATGTACTCAAACACTCCTGCGGACTTCGATTTTAAACTCGAAAGCCTTGCCCAGGGTTTCCAGTCTTTGCCCGAGTTCGCGGAATATCTAGCTGCCTCTGTGGACATAGTGTTCCCAGTCATACATGGTCAATTTGGTGAAGATGGTGGCATTCAGGAGCTTCTGGAAAAGTATAACGTTCCATTTGTGGGCACGGGATCAATTGAATGTTGCCAAGCATTCGACAAATACAATGCTTCATTGGAGCTCAGCAAACAAGGATTCATAACAGTACCAAGTTGCTTAGTACAGGGAAGTGAAGCAGATGAATCTGAACTGTCAAAATGGTTTGCAAGAAATCAACTGGATGTTGAGTCAGGGAAAGTTGTGGTAAAACCAGCACGAGCAGGATCAAGCATTGGTGTTACAGTTGCATATGGTCTGGCTGATACTTATAGCAAGGCTAATGCAATTATTACAGAGGGAATTGACTCCAAAGTCCTTGTAGAAATATTTCTTGAAGGTGGGAGCGAGTTTACAGCAATTGTTCTTGATGTGGGCTCTGGTTTAGATTCCCATCCTGTTGTATTACTCCCAAGCGAGGTAGAACTTCAGTTCCATGACAATGTTGATGAGGATGCAATTTTCAACTACCGGAGGAAATATCTTCCAACACAACAGGTTGCTTATCATACTCCACCTCGGTTTCCTATTGATGTCATTGAAAGTATTCGAGAAGGAGCATCTCAGTTATTCAGAAAGCTTGGTCTTCGTGACTTTGCTCGAATTGATGGATGGTTTCTCCCTAATTCTATTCATGTACCATCATCATCAGATAGCAAATTCGGAAAGACTGAGATGGGTACAATATTGTATACTGACATTAATCTGATTAGTGGGATGGAACAGACCAGCTTTTTATTTCAGCAAGCCTCGAAGGTTGGACTTTCTCATTCAAATATTCTCAGGAGCATAATTCGTCGGGCTTGCATGCGTTATCCGAATCTTGCACCATTTTGTAGTGCATCAGATCATGTTTCTAGAAGATCACAAACGTCACTGCGTAATGAACCGGTTTGCAACGACACTCGTAAAGTTTTTGTTATTTTTGGAGGAGACTCTTCAGAGAGGCAAGTATCTCTTATCAGCGGAACAAATGTTTGGCTCAATTTGCAAGCTTTTGACGATCTTGAAGTGATTCCATGTTTGCTTGCCCCCACAATTGACAAAAACGAAGTTGATGTGAGTTCCAGAACAGTTTGGTCTTTACCTTATTCTCTTGTGCTGAGACACACTGTAGAGGAAGTTCTTGATGCATGTGTAGAGGCAATTGAACCAGACCGAGTTGCATTGACATCTCACTTAAGGAACAGAGTTGTTCATGATCTTATGGAAGGCTTGAAGAAACATTCTTGGTTTACTGGATTTGATATCATTGATGAACTGCCTGTAAAATTCTCGGTTGAACAGTGGATCAAGCTAGCCAAGGAAGTTCACGCTACAGTTTTTCTTTCAGTGCATGGAGGCATTGGTGAAGATGGCACACTTCAGTCATTGTTAGAGGCTGAAGGGATTCCACATACAGGTCCTGGAGTGATGTCTTCAAAGATTTGTATGGACAAACTTGCAACATCGCTTGCTCTTAATCATCTGTCGGATTTGGGAGTTCTTACTATCAACAAAGATTTGAGGAGAAAAGAAGATCTCATTAGTTCATCAACACCAAATGTATGGCATGAATTGACGTCAAAGCTTCACTGTGCAACATTATGTGTTAAACCTGCAAGAGATGGATGCTCAACGGGGGTTGCAAGGTTATGCTGTGCTGAAGACCTTTCGGTGTATGTAAAAGCATTGGAAGACTGTCTTCACCGGATTCCTCCTAATAGTTTTTCTAAGGCACATGGAACTATTGAGATGCCAAACCCCCCTCCAGAGCTCTTAATCTTCGAGCCTTTTATCGAGACCGATAATATAATCTTATCATCAAAATCTAGAAATGAAAAGGGGCATCAGATCTTGTGGAGCGGAGAGAGTAGATGGGTTGAAATAACTGTTGGAGTTGTTGGGAAACAAGGATCAATGCGCTCACTGACTCCTAGCATCACTGTCAGGGAGAGTGGTGACATTTTGACGCTCGAGGAGAAATTCCAAGGTGGGACTGGCATCAATCTGACTCCACCTCCATCATCCATTATTAGCAACGAGGCGTTGCAGAAATGTAAAAAACATATCGAAATAATTGCAAACACTCTTGAATTAGAAGGGTTTTCACGAATAGATGCATTTGTCAACGTGGACAGTGGAGAGGTCTTGATGATAGAGGTCAACACAGTGCCTGGAATGACGCCTTCCACGGTCTTGATTCACCAGGCACTAGCGGAGGAACCACCGATGTACCCTCACGAGTTTTTCCGTACACTGCTCGATTTGGCATCAGAGAGGACCATATATACTTTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dala_Dala_lig_C Dala_Dala_lig_N

Protein Analysis

946

Amino Acids

103.75

Weight (kDa)

5.33

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dala_Dala_lig_N PF01820 80 - 195 2.3e-23 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 252 - 450 4.7e-16 D-ala D-ala ligase C-terminus
Dala_Dala_lig_N PF01820 489 - 654 4.1e-17 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 806 - 912 3.4e-11 D-ala D-ala ligase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 869
AatII GACGTC 1 cut(s) 2120
Acc36I ACCTGC 1 cut(s) 2161
AccBSI CCGCTC 1 cut(s) 2400
AccI GTMKAC 1 cut(s) 1247
AccII CGCG 6 cut(s) 42, 133, 141, 159, 167, 473
AclI AACGTT 1 cut(s) 570
AclWI GGATC 4 cut(s) 598, 790, 1853, 2458
AcsI RAATTY 3 cut(s) 1214, 1826, 2519
AcuI CTGAAG 5 cut(s) 968, 1479, 1904, 1959, 2220
AcyI GRCGYC 3 cut(s) 246, 2117, 2720
AfaI GTAC 8 cut(s) 80, 398, 666, 681, 1194, 1237, 2771, 2791
AfiI CCNNNNNNNGG 4 cut(s) 445, 581, 973, 1955
AflII CTTAAG 1 cut(s) 1735
AflIII ACRYGT 1 cut(s) 334
AgeI ACCGGT 1 cut(s) 1444
AjnI CCWGG 4 cut(s) 444, 1960, 2710, 2742
AjuI GAANNNNNNNTTGG 4 cut(s) 1516, 1548, 2412, 2444
AloI GAACNNNNNNTCC 6 cut(s) 80, 112, 488, 520, 972, 1004
Alw21I GWGCWC 2 cut(s) 642, 2313
Alw26I GTCTC 4 cut(s) 188, 1482, 1654, 2330
AlwI GGATC 4 cut(s) 598, 790, 1853, 2458
AlwNI CAGNNNCTG 2 cut(s) 1961, 2711
Ama87I CYCGRG 2 cut(s) 464, 2510
AoxI GGCC 2 cut(s) 25, 361
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 3 cut(s) 1214, 1826, 2519
AseI ATTAAT 1 cut(s) 1257
AsiGI ACCGGT 1 cut(s) 1444
Asp700I GAANNNNTTC 4 cut(s) 885, 1101, 1569, 1944
AspLEI GCGC 4 cut(s) 44, 141, 161, 2460
AspS9I GGNCC 3 cut(s) 260, 1958, 2817
AsuHPI GGTGA 7 cut(s) 220, 338, 542, 1916, 2234, 2507, 2732
AsuNHI GCTAGC 1 cut(s) 1852
AvaI CYCGRG 2 cut(s) 464, 2510
AvaII GGWCC 3 cut(s) 260, 1958, 2817
BaeGI GKGCMC 1 cut(s) 587
BaeI ACNNNNGTAYC 2 cut(s) 2753, 2786
BanII GRGCYC 3 cut(s) 642, 935, 2313
BauI CACGAG 2 cut(s) 774, 2777
BbsI GAAGAC 5 cut(s) 1133, 1965, 2208, 2231, 2238
Bbv12I GWGCWC 2 cut(s) 642, 2313
BbvI GCAGC 1 cut(s) 472
BceAI ACGGC 1 cut(s) 73
BcgI CGANNNNNNTGC 4 cut(s) 422, 456, 2597, 2631
BciT130I CCWGG 4 cut(s) 446, 1962, 2712, 2744
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 4 cut(s) 188, 1482, 1654, 2330
BfaI CTAG 6 cut(s) 482, 1413, 1853, 2367, 2472, 2750
BfmI CTRYAG 2 cut(s) 1668, 1872
BfrI CTTAAG 1 cut(s) 1735
BfuAI ACCTGC 1 cut(s) 2161
BglII AGATCT 2 cut(s) 2074, 2388
BisI GCNGC 1 cut(s) 486
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 1 cut(s) 487
Bme1390I CCNGG 4 cut(s) 446, 1962, 2712, 2744
Bme18I GGWCC 3 cut(s) 260, 1958, 2817
BmeT110I CYCGRG 2 cut(s) 464, 2510
BmgT120I GGNCC 3 cut(s) 260, 1958, 2817
BmiI GGNNCC 3 cut(s) 22, 262, 2760
BmrFI CCNGG 4 cut(s) 446, 1962, 2712, 2744
BmrI ACTGGG 1 cut(s) 503
BmtI GCTAGC 1 cut(s) 1856
BmuI ACTGGG 1 cut(s) 503
BpiI GAAGAC 5 cut(s) 1133, 1965, 2208, 2231, 2238
BpmI CTGGAG 2 cut(s) 1983, 2289
Bpu1102I GCTNAGC 1 cut(s) 641
BsaBI GATNNNNATC 1 cut(s) 948
BsaHI GRCGYC 3 cut(s) 246, 2117, 2720
BsaI GGTCTC 1 cut(s) 2330
BsaJI CCNNGG 8 cut(s) 165, 264, 444, 445, 1073, 1857, 2524, 2727
BsaWI WCCGGW 3 cut(s) 1026, 1444, 2244
BsaXI ACNNNNNCTCC 4 cut(s) 103, 133, 2539, 2569
Bsc4I CCNNNNNNNGG 4 cut(s) 445, 581, 973, 1955
Bse118I RCCGGY 1 cut(s) 1444
Bse1I ACTGG 5 cut(s) 454, 509, 741, 1798, 2539
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 4 cut(s) 446, 1962, 2712, 2744
BseDI CCNNGG 8 cut(s) 165, 264, 444, 445, 1073, 1857, 2524, 2727
BseGI GGATG 7 cut(s) 745, 949, 1015, 1172, 1278, 2171, 2561
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 4 cut(s) 445, 581, 973, 1955
BseMII CTCAG 6 cut(s) 404, 655, 1133, 1218, 1351, 1649
BseNI ACTGG 5 cut(s) 454, 509, 741, 1798, 2539
BseRI GAGGAG 4 cut(s) 124, 1500, 2077, 2528
BseSI GKGCMC 1 cut(s) 587
BseXI GCAGC 1 cut(s) 472
Bsh1236I CGCG 6 cut(s) 42, 133, 141, 159, 167, 473
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 27, 363
BshTI ACCGGT 1 cut(s) 1444
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 642, 2313
BsiHKCI CYCGRG 2 cut(s) 464, 2510
BsiSI CCGG 4 cut(s) 24, 1027, 1445, 2245
BslFI GGGAC 2 cut(s) 246, 2545
BslI CCNNNNNNNGG 4 cut(s) 445, 581, 973, 1955
BsmAI GTCTC 4 cut(s) 188, 1482, 1654, 2330
BsmBI CGTCTC 1 cut(s) 188
BsmFI GGGAC 2 cut(s) 246, 2545
BsmI GAATGC 2 cut(s) 543, 611
BsnI GGCC 2 cut(s) 27, 363
Bso31I GGTCTC 1 cut(s) 2330
BsoBI CYCGRG 2 cut(s) 464, 2510
Bsp1286I GDGCHC 4 cut(s) 587, 642, 935, 2313
Bsp1720I GCTNAGC 1 cut(s) 641
BspANI GGCC 2 cut(s) 27, 363
BspCNI CTCAG 6 cut(s) 403, 654, 1132, 1219, 1350, 1650
BspFNI CGCG 6 cut(s) 42, 133, 141, 159, 167, 473
BspHI TCATGA 1 cut(s) 1753
BspLI GGNNCC 3 cut(s) 22, 262, 2760
BspMI ACCTGC 1 cut(s) 2161
BspOI GCTAGC 1 cut(s) 1856
BspPI GGATC 4 cut(s) 598, 790, 1853, 2458
BspTI CTTAAG 1 cut(s) 1735
BspTNI GGTCTC 1 cut(s) 2330
BsrBI CCGCTC 1 cut(s) 2400
BsrFI RCCGGY 1 cut(s) 1444
BsrI ACTGG 5 cut(s) 454, 509, 741, 1798, 2539
BssAI RCCGGY 1 cut(s) 1444
BssECI CCNNGG 8 cut(s) 165, 264, 444, 445, 1073, 1857, 2524, 2727
BssNAI GTATAC 1 cut(s) 1248
BssNI GRCGYC 3 cut(s) 246, 2117, 2720
BssSI CACGAG 2 cut(s) 774, 2777
BssT1I CCWWGG 2 cut(s) 1857, 2524
Bst1107I GTATAC 1 cut(s) 1248
Bst2BI CACGAG 2 cut(s) 774, 2777
Bst2UI CCWGG 4 cut(s) 446, 1962, 2712, 2744
Bst6I CTCTTC 1 cut(s) 1498
BstACI GRCGYC 3 cut(s) 246, 2117, 2720
BstAFI CTTAAG 1 cut(s) 1735
BstC8I GCNNGC 9 cut(s) 40, 361, 1302, 1360, 1364, 1548, 1584, 1854, 2010
BstDEI CTNAG 9 cut(s) 236, 390, 641, 676, 1119, 1227, 1337, 1658, 2268
BstDSI CCRYGG 2 cut(s) 165, 2727
BstENI CCTNNNNNAGG 1 cut(s) 443
BstF5I GGATG 7 cut(s) 745, 949, 1015, 1172, 1278, 2171, 2561
BstFNI CGCG 6 cut(s) 42, 133, 141, 159, 167, 473
BstHHI GCGC 4 cut(s) 44, 141, 161, 2460
BstMAI GTCTC 4 cut(s) 188, 1482, 1654, 2330
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 11, 252, 1543
BstNI CCWGG 4 cut(s) 446, 1962, 2712, 2744
BstNSI RCATGY 3 cut(s) 338, 1366, 1692
BstSCI CCNGG 4 cut(s) 444, 1960, 2710, 2742
BstSFI CTRYAG 2 cut(s) 1668, 1872
BstSLI GKGCMC 1 cut(s) 587
BstUI CGCG 6 cut(s) 42, 133, 141, 159, 167, 473
BstV1I GCAGC 1 cut(s) 472
BstV2I GAAGAC 5 cut(s) 1133, 1965, 2208, 2231, 2238
BstX2I RGATCY 2 cut(s) 2074, 2388
BstXI CCANNNNNNTGG 1 cut(s) 2103
BstYI RGATCY 2 cut(s) 2074, 2388
BstZ17I GTATAC 1 cut(s) 1248
BsuRI GGCC 2 cut(s) 27, 363
BtgI CCRYGG 2 cut(s) 165, 2727
BtgZI GCGATG 1 cut(s) 1989
BtsCI GGATG 7 cut(s) 745, 949, 1015, 1172, 1278, 2171, 2561
BtsI GCAGTG 2 cut(s) 1430, 2792
BveI ACCTGC 1 cut(s) 2161
Cac8I GCNNGC 9 cut(s) 40, 361, 1302, 1360, 1364, 1548, 1584, 1854, 2010
CaiI CAGNNNCTG 2 cut(s) 1961, 2711
CciI TCATGA 1 cut(s) 1753
CfoI GCGC 4 cut(s) 44, 141, 161, 2460
Cfr10I RCCGGY 1 cut(s) 1444
Cfr13I GGNCC 3 cut(s) 260, 1958, 2817
Cfr42I CCGCGG 1 cut(s) 168
CseI GACGC 5 cut(s) 139, 235, 365, 2515, 2728
Csp6I GTAC 8 cut(s) 79, 397, 665, 680, 1193, 1236, 2770, 2790
CspAI ACCGGT 1 cut(s) 1444
CviQI GTAC 8 cut(s) 79, 397, 665, 680, 1193, 1236, 2770, 2790
DdeI CTNAG 9 cut(s) 236, 390, 641, 676, 1119, 1227, 1337, 1658, 2268
DraI TTTAAA 2 cut(s) 427, 2832
DrdI GACNNNNNNGTC 1 cut(s) 869
DseDI GACNNNNNNGTC 1 cut(s) 869
Eam1104I CTCTTC 1 cut(s) 1498
EarI CTCTTC 1 cut(s) 1498
EciI GGCGGA 1 cut(s) 284
Ecl136II GAGCTC 2 cut(s) 640, 2311
Eco130I CCWWGG 2 cut(s) 1857, 2524
Eco24I GRGCYC 3 cut(s) 642, 935, 2313
Eco31I GGTCTC 1 cut(s) 2330
Eco32I GATATC 1 cut(s) 1804
Eco47I GGWCC 3 cut(s) 260, 1958, 2817
Eco53kI GAGCTC 2 cut(s) 640, 2311
Eco57I CTGAAG 5 cut(s) 968, 1479, 1904, 1959, 2220
Eco88I CYCGRG 2 cut(s) 464, 2510
EcoICRI GAGCTC 2 cut(s) 640, 2311
EcoNI CCTNNNNNAGG 1 cut(s) 443
EcoO109I RGGNCCY 2 cut(s) 260, 1958
EcoRII CCWGG 4 cut(s) 444, 1960, 2710, 2742
EcoRV GATATC 1 cut(s) 1804
EcoT14I CCWWGG 2 cut(s) 1857, 2524
EcoT22I ATGCAT 2 cut(s) 1690, 2659
EcoT38I GRGCYC 3 cut(s) 642, 935, 2313
ErhI CCWWGG 2 cut(s) 1857, 2524
Esp3I CGTCTC 1 cut(s) 188
FalI AAGNNNNNCTT 6 cut(s) 106, 138, 1497, 1529, 2223, 2255
FaqI GGGAC 2 cut(s) 246, 2545
FauI CCCGC 1 cut(s) 134
FauNDI CATATG 1 cut(s) 808
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 1 cut(s) 1247
Fnu4HI GCNGC 1 cut(s) 486
FokI GGATG 7 cut(s) 752, 936, 1022, 1179, 1285, 2178, 2548
FriOI GRGCYC 3 cut(s) 642, 935, 2313
Fsp4HI GCNGC 1 cut(s) 486
FspBI CTAG 6 cut(s) 482, 1413, 1853, 2367, 2472, 2750
GlaI GCGC 4 cut(s) 43, 140, 160, 2459
GluI GCNGC 1 cut(s) 486
GsuI CTGGAG 2 cut(s) 1983, 2289
HaeIII GGCC 2 cut(s) 27, 363
HapII CCGG 4 cut(s) 24, 1027, 1445, 2245
HgaI GACGC 5 cut(s) 139, 235, 365, 2515, 2728
HhaI GCGC 4 cut(s) 44, 141, 161, 2460
Hin1I GRCGYC 3 cut(s) 246, 2117, 2720
Hin6I GCGC 4 cut(s) 42, 139, 159, 2458
HinP1I GCGC 4 cut(s) 42, 139, 159, 2458
HincII GTYRAC 3 cut(s) 355, 2665, 2701
HindII GTYRAC 3 cut(s) 355, 2665, 2701
HindIII AAGCTT 3 cut(s) 1133, 1548, 2123
HpaII CCGG 4 cut(s) 24, 1027, 1445, 2245
HphI GGTGA 7 cut(s) 220, 338, 542, 1916, 2234, 2507, 2732
Hpy99I CGWCG 6 cut(s) 10, 59, 130, 133, 251, 1356
HpyCH4IV ACGT 5 cut(s) 351, 570, 1427, 2117, 2667
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 252, 1543
HpyF3I CTNAG 9 cut(s) 236, 390, 641, 676, 1119, 1227, 1337, 1658, 2268
HpySE526I ACGT 5 cut(s) 351, 570, 1427, 2117, 2667
Hsp92I GRCGYC 3 cut(s) 246, 2117, 2720
HspAI GCGC 4 cut(s) 42, 139, 159, 2458
Ksp22I TGATCA 1 cut(s) 310
KspI CCGCGG 1 cut(s) 168
LmnI GCTCC 7 cut(s) 271, 550, 637, 900, 1112, 1341, 2397
Lsp1109I GCAGC 1 cut(s) 472
MaeI CTAG 6 cut(s) 482, 1413, 1853, 2367, 2472, 2750
MaeII ACGT 5 cut(s) 351, 570, 1427, 2117, 2667
MaeIII GTNAC 5 cut(s) 151, 796, 1145, 1428, 2495
MbiI CCGCTC 1 cut(s) 2400
MfeI CAATTG 4 cut(s) 594, 915, 1593, 1701
MflI RGATCY 2 cut(s) 2074, 2388
MhlI GDGCHC 4 cut(s) 587, 642, 935, 2313
MlyI GAGTC 6 cut(s) 159, 755, 857, 1484, 2461, 2542
MmeI TCCRAC 5 cut(s) 127, 1067, 1294, 2007, 2410
Mph1103I ATGCAT 2 cut(s) 1690, 2659
MroXI GAANNNNTTC 4 cut(s) 885, 1101, 1569, 1944
MseI TTAA 8 cut(s) 366, 426, 1257, 1736, 2016, 2148, 2315, 2831
MslI CAYNNNNRTG 1 cut(s) 996
MspA1I CMGCKG 2 cut(s) 167, 1521
MspCI CTTAAG 1 cut(s) 1735
MspI CCGG 4 cut(s) 24, 1027, 1445, 2245
MspR9I CCNGG 4 cut(s) 446, 1962, 2712, 2744
MunI CAATTG 4 cut(s) 594, 915, 1593, 1701
Mva1269I GAATGC 2 cut(s) 543, 611
MvaI CCWGG 4 cut(s) 446, 1962, 2712, 2744
MvnI CGCG 6 cut(s) 42, 133, 141, 159, 167, 473
MwoI GCNNNNNNNGC 3 cut(s) 11, 252, 1543
NdeI CATATG 1 cut(s) 808
NheI GCTAGC 1 cut(s) 1852
NlaIV GGNNCC 3 cut(s) 22, 262, 2760
NmeAIII GCCGAG 3 cut(s) 70, 245, 272
NmuCI GTSAC 4 cut(s) 151, 1145, 1428, 2495
NsiI ATGCAT 2 cut(s) 1690, 2659
NspI RCATGY 3 cut(s) 338, 1366, 1692
PaeI GCATGC 1 cut(s) 1366
PaeR7I CTCGAG 1 cut(s) 2510
PagI TCATGA 1 cut(s) 1753
PasI CCCWGGG 1 cut(s) 445
PciI ACATGT 1 cut(s) 334
PctI GAATGC 2 cut(s) 543, 611
PdmI GAANNNNTTC 4 cut(s) 885, 1101, 1569, 1944
PfeI GAWTC 8 cut(s) 654, 701, 944, 1375, 1570, 1945, 2248, 2737
PfoI TCCNGGA 1 cut(s) 1960
PinAI ACCGGT 1 cut(s) 1444
PkrI GCNGC 1 cut(s) 487
Ple19I CGATCG 1 cut(s) 12
PleI GAGTC 6 cut(s) 158, 754, 857, 1484, 2461, 2542
PpsI GAGTC 6 cut(s) 158, 754, 857, 1484, 2461, 2542
PpuMI RGGWCCY 2 cut(s) 260, 1958
PscI ACATGT 1 cut(s) 334
PshBI ATTAAT 1 cut(s) 1257
Psp124BI GAGCTC 2 cut(s) 642, 2313
Psp1406I AACGTT 1 cut(s) 570
Psp5II RGGWCCY 2 cut(s) 260, 1958
Psp6I CCWGG 4 cut(s) 444, 1960, 2710, 2742
PspGI CCWGG 4 cut(s) 444, 1960, 2710, 2742
PspN4I GGNNCC 3 cut(s) 22, 262, 2760
PspPI GGNCC 3 cut(s) 260, 1958, 2817
PspPPI RGGWCCY 2 cut(s) 260, 1958
PspXI VCTCGAGB 1 cut(s) 2510
PsrI GAACNNNNNNTAC 2 cut(s) 1662, 1694
PstNI CAGNNNCTG 2 cut(s) 1961, 2711
PsuI RGATCY 2 cut(s) 2074, 2388
PvuI CGATCG 1 cut(s) 12
RsaI GTAC 8 cut(s) 80, 398, 666, 681, 1194, 1237, 2771, 2791
RsaNI GTAC 8 cut(s) 79, 397, 665, 680, 1193, 1236, 2770, 2790
RseI CAYNNNNRTG 1 cut(s) 996
SacI GAGCTC 2 cut(s) 642, 2313
SacII CCGCGG 1 cut(s) 168
SaqAI TTAA 8 cut(s) 366, 426, 1257, 1736, 2016, 2148, 2315, 2831
SatI GCNGC 1 cut(s) 486
Sau96I GGNCC 3 cut(s) 260, 1958, 2817
SchI GAGTC 6 cut(s) 159, 755, 857, 1484, 2461, 2542
ScrFI CCNGG 4 cut(s) 446, 1962, 2712, 2744
SduI GDGCHC 4 cut(s) 587, 642, 935, 2313
SfcI CTRYAG 2 cut(s) 1668, 1872
Sfr274I CTCGAG 1 cut(s) 2510
Sfr303I CCGCGG 1 cut(s) 168
SgrBI CCGCGG 1 cut(s) 168
SinI GGWCC 3 cut(s) 260, 1958, 2817
SlaI CTCGAG 1 cut(s) 2510
SmiMI CAYNNNNRTG 1 cut(s) 996
SmlI CTYRAG 2 cut(s) 1735, 2510
SmoI CTYRAG 2 cut(s) 1735, 2510
SphI GCATGC 1 cut(s) 1366
SspI AATATT 3 cut(s) 885, 1242, 1333
SspMI CTAG 6 cut(s) 482, 1413, 1853, 2367, 2472, 2750
SstI GAGCTC 2 cut(s) 642, 2313
StyD4I CCNGG 4 cut(s) 444, 1960, 2710, 2742
StyI CCWWGG 2 cut(s) 1857, 2524
TaiI ACGT 5 cut(s) 354, 573, 1430, 2120, 2670
TaqII GACCGA 2 cut(s) 1728, 2354
TatI WGTACW 3 cut(s) 78, 396, 679
TfiI GAWTC 8 cut(s) 654, 701, 944, 1375, 1570, 1945, 2248, 2737
Tru1I TTAA 8 cut(s) 366, 426, 1257, 1736, 2016, 2148, 2315, 2831
Tru9I TTAA 8 cut(s) 366, 426, 1257, 1736, 2016, 2148, 2315, 2831
TseFI GTSAC 4 cut(s) 151, 1145, 1428, 2495
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 4 cut(s) 151, 1145, 1428, 2495
TspGWI ACGGA 2 cut(s) 212, 2777
Vha464I CTTAAG 1 cut(s) 1735
VpaK11BI GGWCC 3 cut(s) 260, 1958, 2817
VspI ATTAAT 1 cut(s) 1257
XagI CCTNNNNNAGG 1 cut(s) 443
XapI RAATTY 3 cut(s) 1214, 1826, 2519
XbaI TCTAGA 2 cut(s) 1412, 2366
XceI RCATGY 3 cut(s) 338, 1366, 1692
XcmI CCANNNNNNNNNTGG 1 cut(s) 2531
XhoI CTCGAG 1 cut(s) 2510
XmiI GTMKAC 1 cut(s) 1247
XmnI GAANNNNTTC 4 cut(s) 885, 1101, 1569, 1944
XspI CTAG 6 cut(s) 482, 1413, 1853, 2367, 2472, 2750
ZraI GACGTC 1 cut(s) 2118
Zsp2I ATGCAT 2 cut(s) 1690, 2659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.