Rh3DG111400

D-ala D-ala ligase N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005073
Physical Location & Seq
Reverse (-)
9125283 .. 9135448
10166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG111400.1

Sequence Viewer

Length: 2868 bp
ATGGCCAGCCTCGCATTCGGTTCCAGCCTCACTTTACTTCGCGCCAACCAAAACGACGCCGCACAGAATAAGCCTCTTATCTACACTCCCAATTTCATCTCTCTCCGATTCAACCACAGCCTCAGAACCCACGCTTCGTCGTCGACGCGGTGCCGCGGCGTCGGAGCTCCACGCGCCGCCGCGGAGGTGGTGGAGGATGTAACTGTCGAGGGAGAGGAGAAGGAGAATCGTAGGGTTCTGAGAGTCGGGGTCATCTGCGGTGGCCCCTCCGCCGAGCGTGGAATCTCTCTCAACTCCGCTAGATCAGTAATCGATCATATTCAAGGAGAGGATTTACATGTGAGTTGCTACTACATTGACTCTGAACTCAACGCTTTTGCAATTTCCCCTGCTCAGGTGTACTCCAACACTCCGGCGGATTTTGATTTCAAACTCGAAAGCCTTGCTCAAGGTTTCAAGTCTTGGTCTGACTTTGCGGACCATCTTGCTGTCAATGTGGACATAGTGTTTCCAGTCATACATGGTCAATTTGGTGAAGATGGCGGCATTCAGGAGGTGTTGGAAAGGCATAACATTCCATTTGTTGGCACGGGATCAAATGAGTGTCGTCAAGCATTCGACAAGTACAATGCCTCATTGGAGCTCAGCAGACATGGATTTGTAACTGTACCAAGTTGTTTAGTACAGGGCAGTAAAGCAGATGAACCCAAACTGTCAGAATGGTTTGCAAAAAATCAACTGGATCCTAAGTCCGGAAAAGTTGTGGTAAAACCAGCACGAGCAGGTTCAAGCATTGGTGTTACAGTTGCATATGGCTTGGCTGATTCTCTTAGCAAGGCTAATGCAATTATTACAGAGGGAATTGATTCCAAAGTCCTTGTTGAAATATTTCTTGAAGGAGGGAGTGAATTTACAGCAATTGTTCTTGATGTGGGGTATGGTACAGATTCTCATCCTGTTGTACTATTACCAACTGAGGTGGAACTTCAATTCCTTGGTAGTGTAGATGTTAGAGAGAAGGATGCAATCTTCAACTACCGCAGGAAATATCTTCCAACACAACAGGTTGCTTATCATACTCCACCTCGTTTTCCTATTGATGTCATCCAAAATATTCGAGATGGAGCATCTCAGTTATTCCAAAAGCTTGGCCTTCGTGACTTTGCTCGAATCGATGGATGGTTTCTGCCCAATTCTATTCATGTACCATCATCGCCAGATAGCAAGTTTGGAAGGACTGAGATGGGCACAATATTATACACTGACATTAACCTGATTAGTGGTATGGAGCAGACCAGCTTTTTATTCCAGCAAGCCTCAAAGGTTGGGTTTTCTCATGCAAATATTCTCCGGAGCATCATTAATCATGCTTGTTTGCGATTTCCGCATCTTGCATCTTGTGATGTGTCTAGTGATTTGCCTAGAACATCAAAATCCCCACTGCTTAAAGATGATTGGGAAGGCACTCAGAAAGTTTTTGTTATTTTTGGAGGAGACACATCAGAGAGGCAAGTATCTCTTATGAGCGGAACAAATGTTTGGCTTAATTTGCAAGCTTTTGATGATCTGGAAGTGCTTCCTTGCTTGCTTGCCCCCACGAATGGATATTCGTCCAGTACTGATGTGGACAAAAATGAAGTTGATGCAACTTCCCAAACAGTTTGGTCATTACCTTATTCTCTTGTGCTAAGACACACCACGGAGGAAGTTCTTGCTGCATGTGTAGAGGCAATAGAACCAGACCGAGCTGCACTTACATCTCAATTACGGAACAGAGTTATTGATGATCTTACGGAAGGCTTCAAGAAACATTCTTGGTTTACAGGATTTGATATCACTGATGAACTGCCTGTTAAATTTTCTATTGAAGAGTGGATAAAGCTAGCCAAGGAAGTCAAGGCAACAGTATTTATTGCAGTGCATGGAGGCATTGGTGAAGATGGTACACTTCAGTCTTTGTTGGAGGCTGAAGGGATTCCACATACAGGTCCTGGTGTTTTGGCTTCAAAGATTTGTATGGACAAAGTCGCAACATCAGTTGCTCTTAAACATCTATCGGACTTGGGAGTTCTTACCATAAACAAAGATTTGAGAAGAAGAGATCAGCTCCTTAATACACCAATACCAAATGTATGGCATGAATTGACCTCCAAGCTTCAGTGTGAAACATTATGTGTTAAACCGGCAAGAGATGGATGCTCAACTGGAGTTGCAAGGTTATGCTGTGATGAAGACCTTTCAGTGTACGTAAAAGCATTGGAGGACTGTCTCCTTCGGATTCCCCCTAATAGTTTGTCAAAGGAACACGGAATGATCGAAATGCCAAACCCCGCTCCAGAGCTCCTAATCTTTGAACCTTTTATTGATACGGATGATATAATTGTTTCATTGAAGTCCATAAATGAAAATGGACATCACCTCATGTGGAATGGACAGAGTAGATGGGTTGAAATAACTGTTGGTGTTATTGGGAAACAAGGATCAATGCACTCACTGAGTCCCAGTATCACTGTGAAGGAGAGTGGTGATATTTTATCACTTGAGGAGAAATTTCAGGGTGGGACTGGCATCAATCTTACTCCACCTCCGTCATCGATCATTAGCCATGAGGCCTTGCAGAAATGTAAACAACACATTGAAATGATTGCCAACACTTTAGAATTAGAAGGATTTTCACGAATTGATGCCTTTGTCAACGTGGATAGTGGAGAGGTGCTGGTTATAGAGGTCAATACAGTGCCTGGAATGACACCTTCCACTGTTCTGATTCATCAGGTATTCAATTTGGAAACACATTTTCTTTTGGGGGAAATTGAATCAGCTCATTTTGGTTTCGCATTGCAGCAACGGAAACATATTTTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dala_Dala_lig_C Dala_Dala_lig_N

Protein Analysis

955

Amino Acids

104.84

Weight (kDa)

5.26

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dala_Dala_lig_N PF01820 81 - 196 5.3e-24 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 254 - 392 3.4e-13 D-ala D-ala ligase C-terminus
Dala_Dala_lig_N PF01820 491 - 664 9.7e-18 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 816 - 921 1.8e-11 D-ala D-ala ligase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 773
AccB1I GGYRCC 1 cut(s) 150
AccB7I CCANNNNNTGG 1 cut(s) 584
AccBSI CCGCTC 2 cut(s) 1527, 2333
AccI GTMKAC 1 cut(s) 143
AccII CGCG 5 cut(s) 42, 148, 156, 174, 182
AccIII TCCGGA 2 cut(s) 752, 1350
AclWI GGATC 4 cut(s) 601, 737, 750, 2488
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 908, 1856, 2549
AcuI CTGAAG 3 cut(s) 1934, 1989, 2141
AcyI GRCGYC 2 cut(s) 57, 159
AfiI CCNNNNNNNGG 5 cut(s) 394, 584, 752, 1601, 1985
AflIII ACRYGT 1 cut(s) 337
AjnI CCWGG 2 cut(s) 1990, 2740
AjuI GAANNNNNNNTTGG 4 cut(s) 1522, 1554, 2442, 2474
AloI GAACNNNNNNTCC 2 cut(s) 975, 1007
Alw21I GWGCWC 3 cut(s) 169, 645, 2343
Alw26I GTCTC 2 cut(s) 1488, 2273
AlwI GGATC 4 cut(s) 601, 737, 750, 2488
AlwNI CAGNNNCTG 2 cut(s) 1991, 2741
Aor13HI TCCGGA 2 cut(s) 752, 1350
AoxI GGCC 4 cut(s) 3, 262, 1150, 2610
ApeKI GCWGC 3 cut(s) 1715, 1748, 2842
ApoI RAATTY 3 cut(s) 908, 1856, 2549
AseI ATTAAT 1 cut(s) 1362
Asp700I GAANNNNTTC 4 cut(s) 865, 888, 1575, 1974
AspLEI GCGC 2 cut(s) 44, 176
AspS9I GGNCC 3 cut(s) 263, 478, 1988
AsuHPI GGTGA 4 cut(s) 545, 1946, 2408, 2537
AsuNHI GCTAGC 1 cut(s) 1882
AvaII GGWCC 2 cut(s) 478, 1988
BaeGI GKGCMC 1 cut(s) 1250
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 742
BanI GGYRCC 1 cut(s) 150
BanII GRGCYC 3 cut(s) 169, 645, 2343
BauI CACGAG 1 cut(s) 777
BbsI GAAGAC 1 cut(s) 2238
Bbv12I GWGCWC 3 cut(s) 169, 645, 2343
BbvI GCAGC 3 cut(s) 1702, 1735, 2854
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 2 cut(s) 1992, 2742
BcoDI GTCTC 2 cut(s) 1488, 2273
BfaI CTAG 4 cut(s) 300, 1410, 1422, 1883
BfuAI ACCTGC 1 cut(s) 773
BisI GCNGC 9 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749, 2843
BlpI GCTNAGC 1 cut(s) 644
BlsI GCNGC 9 cut(s) 61, 155, 158, 178, 181, 545, 1717, 1750, 2844
BmcAI AGTACT 1 cut(s) 1618
Bme1390I CCNGG 2 cut(s) 1992, 2742
Bme18I GGWCC 2 cut(s) 478, 1988
BmgT120I GGNCC 3 cut(s) 263, 478, 1988
BmiI GGNNCC 4 cut(s) 22, 152, 265, 744
BmrFI CCNGG 2 cut(s) 1992, 2742
BmrI ACTGGG 1 cut(s) 2496
BmsI GCATC 9 cut(s) 1012, 1136, 1365, 1396, 1403, 1633, 2186, 2577, 2674
BmtI GCTAGC 1 cut(s) 1886
BmuI ACTGGG 1 cut(s) 2496
BpiI GAAGAC 1 cut(s) 2238
BplI GAGNNNNNCTC 2 cut(s) 2091, 2123
BpmI CTGGAG 2 cut(s) 2226, 2319
Bpu10I CCTNAGC 1 cut(s) 393
Bpu1102I GCTNAGC 1 cut(s) 644
BpuEI CTTGAG 2 cut(s) 432, 2561
Bsa29I ATCGAT 3 cut(s) 312, 1173, 2594
BsaAI YACGTR 1 cut(s) 2248
BsaBI GATNNNNATC 1 cut(s) 951
BsaHI GRCGYC 2 cut(s) 57, 159
BsaJI CCNNGG 5 cut(s) 154, 180, 994, 1698, 1887
BsaWI WCCGGW 2 cut(s) 752, 1350
BsaXI ACNNNNNCTCC 2 cut(s) 2569, 2599
Bsc4I CCNNNNNNNGG 5 cut(s) 394, 584, 752, 1601, 1985
Bse118I RCCGGY 1 cut(s) 2182
Bse1I ACTGG 6 cut(s) 512, 744, 1614, 2209, 2502, 2569
Bse3DI GCAATG 1 cut(s) 2837
Bse8I GATNNNNATC 1 cut(s) 951
BseAI TCCGGA 2 cut(s) 752, 1350
BseBI CCWGG 2 cut(s) 1992, 2742
BseCI ATCGAT 3 cut(s) 312, 1173, 2594
BseDI CCNNGG 5 cut(s) 154, 180, 994, 1698, 1887
BseGI GGATG 7 cut(s) 202, 952, 1027, 1104, 1184, 2201, 2377
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 5 cut(s) 394, 584, 752, 1601, 1985
BseMI GCAATG 1 cut(s) 2837
BseMII CTCAG 9 cut(s) 136, 230, 407, 658, 966, 1145, 1230, 1481, 2486
BseNI ACTGG 6 cut(s) 512, 744, 1614, 2209, 2502, 2569
BseRI GAGGAG 3 cut(s) 230, 1506, 2558
BseSI GKGCMC 1 cut(s) 1250
BseXI GCAGC 3 cut(s) 1702, 1735, 2854
BsgI GTGCAG 1 cut(s) 1734
Bsh1236I CGCG 5 cut(s) 42, 148, 156, 174, 182
BshFI GGCC 4 cut(s) 5, 264, 1152, 2612
BshNI GGYRCC 1 cut(s) 150
BshVI ATCGAT 3 cut(s) 312, 1173, 2594
BsiHKAI GWGCWC 3 cut(s) 169, 645, 2343
BsiSI CCGG 4 cut(s) 413, 753, 1351, 2183
BslFI GGGAC 2 cut(s) 2484, 2575
BslI CCNNNNNNNGG 5 cut(s) 394, 584, 752, 1601, 1985
BsmAI GTCTC 2 cut(s) 1488, 2273
BsmFI GGGAC 2 cut(s) 2484, 2575
BsmI GAATGC 3 cut(s) 14, 546, 614
BsnI GGCC 4 cut(s) 5, 264, 1152, 2612
Bsp1286I GDGCHC 4 cut(s) 169, 645, 1250, 2343
Bsp13I TCCGGA 2 cut(s) 752, 1350
Bsp1720I GCTNAGC 1 cut(s) 644
BspANI GGCC 4 cut(s) 5, 264, 1152, 2612
BspCNI CTCAG 9 cut(s) 135, 231, 406, 657, 967, 1144, 1231, 1480, 2487
BspDI ATCGAT 3 cut(s) 312, 1173, 2594
BspEI TCCGGA 2 cut(s) 752, 1350
BspFNI CGCG 5 cut(s) 42, 148, 156, 174, 182
BspLI GGNNCC 4 cut(s) 22, 152, 265, 744
BspMI ACCTGC 1 cut(s) 773
BspOI GCTAGC 1 cut(s) 1886
BspPI GGATC 4 cut(s) 601, 737, 750, 2488
BspT107I GGYRCC 1 cut(s) 150
BsrBI CCGCTC 2 cut(s) 1527, 2333
BsrDI GCAATG 1 cut(s) 2837
BsrFI RCCGGY 1 cut(s) 2182
BsrI ACTGG 6 cut(s) 512, 744, 1614, 2209, 2502, 2569
BssAI RCCGGY 1 cut(s) 2182
BssECI CCNNGG 5 cut(s) 154, 180, 994, 1698, 1887
BssNI GRCGYC 2 cut(s) 57, 159
BssSI CACGAG 1 cut(s) 777
BssT1I CCWWGG 2 cut(s) 994, 1887
Bst2BI CACGAG 1 cut(s) 777
Bst2UI CCWGG 2 cut(s) 1992, 2742
Bst6I CTCTTC 2 cut(s) 1863, 2092
BstACI GRCGYC 2 cut(s) 57, 159
BstBAI YACGTR 1 cut(s) 2248
BstC8I GCNNGC 6 cut(s) 7, 1314, 1554, 1586, 1590, 1884
BstDSI CCRYGG 3 cut(s) 154, 180, 1698
BstF5I GGATG 7 cut(s) 202, 952, 1027, 1104, 1184, 2201, 2377
BstFNI CGCG 5 cut(s) 42, 148, 156, 174, 182
BstHHI GCGC 2 cut(s) 44, 176
BstMAI GTCTC 2 cut(s) 1488, 2273
BstMWI GCNNNNNNNGC 4 cut(s) 11, 173, 1384, 1549
BstNI CCWGG 2 cut(s) 1992, 2742
BstNSI RCATGY 2 cut(s) 341, 1722
BstSCI CCNGG 2 cut(s) 1990, 2740
BstSLI GKGCMC 1 cut(s) 1250
BstSNI TACGTA 1 cut(s) 2248
BstUI CGCG 5 cut(s) 42, 148, 156, 174, 182
BstV1I GCAGC 3 cut(s) 1702, 1735, 2854
BstV2I GAAGAC 1 cut(s) 2238
BstX2I RGATCY 1 cut(s) 742
BstXI CCANNNNNNTGG 2 cut(s) 1148, 2133
BstYI RGATCY 1 cut(s) 742
Bsu15I ATCGAT 3 cut(s) 312, 1173, 2594
BsuRI GGCC 4 cut(s) 5, 264, 1152, 2612
BsuTUI ATCGAT 3 cut(s) 312, 1173, 2594
BtgI CCRYGG 3 cut(s) 154, 180, 1698
BtgZI GCGATG 1 cut(s) 1197
BtsCI GGATG 7 cut(s) 202, 952, 1027, 1104, 1184, 2201, 2377
BtsI GCAGTG 2 cut(s) 1439, 1923
BveI ACCTGC 1 cut(s) 773
Cac8I GCNNGC 6 cut(s) 7, 1314, 1554, 1586, 1590, 1884
CaiI CAGNNNCTG 2 cut(s) 1991, 2741
CfoI GCGC 2 cut(s) 44, 176
Cfr10I RCCGGY 1 cut(s) 2182
Cfr13I GGNCC 3 cut(s) 263, 478, 1988
Cfr42I CCGCGG 2 cut(s) 157, 183
ClaI ATCGAT 3 cut(s) 312, 1173, 2594
CseI GACGC 3 cut(s) 65, 148, 154
CspCI CAANNNNNGTGG 2 cut(s) 960, 995
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 1863, 2092
EarI CTCTTC 2 cut(s) 1863, 2092
EciI GGCGGA 2 cut(s) 259, 431
Ecl136II GAGCTC 3 cut(s) 167, 643, 2341
Eco105I TACGTA 1 cut(s) 2248
Eco130I CCWWGG 2 cut(s) 994, 1887
Eco147I AGGCCT 1 cut(s) 2612
Eco24I GRGCYC 3 cut(s) 169, 645, 2343
Eco32I GATATC 1 cut(s) 1834
Eco47I GGWCC 2 cut(s) 478, 1988
Eco53kI GAGCTC 3 cut(s) 167, 643, 2341
Eco57I CTGAAG 3 cut(s) 1934, 1989, 2141
EcoICRI GAGCTC 3 cut(s) 167, 643, 2341
EcoO109I RGGNCCY 1 cut(s) 1988
EcoRII CCWGG 2 cut(s) 1990, 2740
EcoRV GATATC 1 cut(s) 1834
EcoT14I CCWWGG 2 cut(s) 994, 1887
EcoT38I GRGCYC 3 cut(s) 169, 645, 2343
ErhI CCWWGG 2 cut(s) 994, 1887
FalI AAGNNNNNCTT 2 cut(s) 1503, 1535
FaqI GGGAC 2 cut(s) 2484, 2575
FauI CCCGC 1 cut(s) 2338
FauNDI CATATG 1 cut(s) 811
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 9 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749, 2843
FokI GGATG 7 cut(s) 209, 939, 1034, 1091, 1191, 2208, 2384
FriOI GRGCYC 3 cut(s) 169, 645, 2343
Fsp4HI GCNGC 9 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749, 2843
FspBI CTAG 4 cut(s) 300, 1410, 1422, 1883
GlaI GCGC 2 cut(s) 43, 175
GluI GCNGC 9 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749, 2843
GsuI CTGGAG 2 cut(s) 2226, 2319
HaeIII GGCC 4 cut(s) 5, 264, 1152, 2612
HapII CCGG 4 cut(s) 413, 753, 1351, 2183
HgaI GACGC 3 cut(s) 65, 148, 154
HhaI GCGC 2 cut(s) 44, 176
Hin1I GRCGYC 2 cut(s) 57, 159
Hin6I GCGC 2 cut(s) 42, 174
HinP1I GCGC 2 cut(s) 42, 174
HincII GTYRAC 2 cut(s) 144, 2695
HindII GTYRAC 2 cut(s) 144, 2695
HindIII AAGCTT 3 cut(s) 1145, 1554, 2153
HpaII CCGG 4 cut(s) 413, 753, 1351, 2183
HphI GGTGA 4 cut(s) 545, 1946, 2408, 2537
Hpy166II GTNNAC 9 cut(s) 144, 400, 499, 1627, 1821, 1946, 2245, 2627, 2695
Hpy8I GTNNAC 9 cut(s) 144, 400, 499, 1627, 1821, 1946, 2245, 2627, 2695
Hpy99I CGWCG 5 cut(s) 59, 142, 145, 148, 164
HpyCH4IV ACGT 2 cut(s) 2247, 2697
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 173, 1384, 1549
HpySE526I ACGT 2 cut(s) 2247, 2697
Hsp92I GRCGYC 2 cut(s) 57, 159
HspAI GCGC 2 cut(s) 42, 174
Kpn2I TCCGGA 2 cut(s) 752, 1350
KspI CCGCGG 2 cut(s) 157, 183
LmnI GCTCC 9 cut(s) 164, 172, 640, 1124, 1288, 1353, 2112, 2338, 2346
Lsp1109I GCAGC 3 cut(s) 1702, 1735, 2854
LweI GCATC 9 cut(s) 1012, 1136, 1365, 1396, 1403, 1633, 2186, 2577, 2674
MaeI CTAG 4 cut(s) 300, 1410, 1422, 1883
MaeII ACGT 2 cut(s) 2247, 2697
MaeIII GTNAC 4 cut(s) 199, 661, 799, 1157
MbiI CCGCTC 2 cut(s) 1527, 2333
MboII GAAGA 8 cut(s) 548, 1021, 1043, 1880, 1949, 2106, 2109, 2243
MfeI CAATTG 1 cut(s) 918
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 4 cut(s) 169, 645, 1250, 2343
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 252, 353, 2506
MmeI TCCRAC 5 cut(s) 142, 429, 540, 1079, 1941
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 752, 1350
MroXI GAANNNNTTC 4 cut(s) 865, 888, 1575, 1974
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 1269, 1362, 1446, 1545, 1854, 2046, 2112, 2178
MslI CAYNNNNRTG 1 cut(s) 2639
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 156, 182
MspI CCGG 4 cut(s) 413, 753, 1351, 2183
MspR9I CCNGG 2 cut(s) 1992, 2742
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 3 cut(s) 14, 546, 614
MvaI CCWGG 2 cut(s) 1992, 2742
MvnI CGCG 5 cut(s) 42, 148, 156, 174, 182
MwoI GCNNNNNNNGC 4 cut(s) 11, 173, 1384, 1549
NdeI CATATG 1 cut(s) 811
NheI GCTAGC 1 cut(s) 1882
NlaIV GGNNCC 4 cut(s) 22, 152, 265, 744
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 1157
NspI RCATGY 2 cut(s) 341, 1722
PceI AGGCCT 1 cut(s) 2612
PciI ACATGT 1 cut(s) 337
PcsI WCGNNNNNNNCGW 1 cut(s) 2313
PctI GAATGC 3 cut(s) 14, 546, 614
PdmI GAANNNNTTC 4 cut(s) 865, 888, 1575, 1974
PflFI GACNNNGTC 1 cut(s) 2024
PflMI CCANNNNNTGG 1 cut(s) 584
PkrI GCNGC 9 cut(s) 61, 155, 158, 178, 181, 545, 1717, 1750, 2844
PleI GAGTC 3 cut(s) 251, 353, 2505
PpsI GAGTC 3 cut(s) 251, 353, 2505
Ppu21I YACGTR 1 cut(s) 2248
PpuMI RGGWCCY 1 cut(s) 1988
PscI ACATGT 1 cut(s) 337
PshBI ATTAAT 1 cut(s) 1362
Psp124BI GAGCTC 3 cut(s) 169, 645, 2343
Psp5II RGGWCCY 1 cut(s) 1988
Psp6I CCWGG 2 cut(s) 1990, 2740
PspGI CCWGG 2 cut(s) 1990, 2740
PspN4I GGNNCC 4 cut(s) 22, 152, 265, 744
PspPI GGNCC 3 cut(s) 263, 478, 1988
PspPPI RGGWCCY 1 cut(s) 1988
PstNI CAGNNNCTG 2 cut(s) 1991, 2741
PsuI RGATCY 1 cut(s) 742
PsyI GACNNNGTC 1 cut(s) 2024
RseI CAYNNNNRTG 1 cut(s) 2639
SacI GAGCTC 3 cut(s) 169, 645, 2343
SacII CCGCGG 2 cut(s) 157, 183
SalI GTCGAC 1 cut(s) 142
SaqAI TTAA 8 cut(s) 1269, 1362, 1446, 1545, 1854, 2046, 2112, 2178
SatI GCNGC 9 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749, 2843
Sau96I GGNCC 3 cut(s) 263, 478, 1988
ScaI AGTACT 1 cut(s) 1618
SchI GAGTC 3 cut(s) 252, 353, 2506
ScrFI CCNGG 2 cut(s) 1992, 2742
SduI GDGCHC 4 cut(s) 169, 645, 1250, 2343
SfaNI GCATC 9 cut(s) 1012, 1136, 1365, 1396, 1403, 1633, 2186, 2577, 2674
Sfr303I CCGCGG 2 cut(s) 157, 183
SgrBI CCGCGG 2 cut(s) 157, 183
SgrDI CGTCGACG 1 cut(s) 142
SinI GGWCC 2 cut(s) 478, 1988
SmiMI CAYNNNNRTG 1 cut(s) 2639
SmlI CTYRAG 2 cut(s) 447, 2540
SmoI CTYRAG 2 cut(s) 447, 2540
SnaBI TACGTA 1 cut(s) 2248
SseBI AGGCCT 1 cut(s) 2612
SspI AATATT 4 cut(s) 888, 1114, 1254, 1345
SspMI CTAG 4 cut(s) 300, 1410, 1422, 1883
SstI GAGCTC 3 cut(s) 169, 645, 2343
StuI AGGCCT 1 cut(s) 2612
StyD4I CCNGG 2 cut(s) 1990, 2740
StyI CCWWGG 2 cut(s) 994, 1887
TaiI ACGT 2 cut(s) 2250, 2700
TaqII GACCGA 1 cut(s) 1758
TatI WGTACW 5 cut(s) 399, 624, 682, 961, 1616
TauI GCSGC 6 cut(s) 62, 156, 159, 179, 182, 546
Tru1I TTAA 8 cut(s) 1269, 1362, 1446, 1545, 1854, 2046, 2112, 2178
Tru9I TTAA 8 cut(s) 1269, 1362, 1446, 1545, 1854, 2046, 2112, 2178
TseFI GTSAC 1 cut(s) 1157
TseI GCWGC 3 cut(s) 1715, 1748, 2842
Tsp45I GTSAC 1 cut(s) 1157
TspGWI ACGGA 7 cut(s) 1715, 1783, 1808, 2322, 2384, 2577, 2863
Tth111I GACNNNGTC 1 cut(s) 2024
Van91I CCANNNNNTGG 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 478, 1988
VspI ATTAAT 1 cut(s) 1362
XapI RAATTY 3 cut(s) 908, 1856, 2549
XceI RCATGY 2 cut(s) 341, 1722
XcmI CCANNNNNNNNNTGG 1 cut(s) 1621
XmiI GTMKAC 1 cut(s) 143
XmnI GAANNNNTTC 4 cut(s) 865, 888, 1575, 1974
XspI CTAG 4 cut(s) 300, 1410, 1422, 1883
ZrmI AGTACT 1 cut(s) 1618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.