RchiOBHm_Chr3g0461101

D-ala D-ala ligase N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005073
Physical Location & Seq
Reverse (-)
9163643 .. 9172076
8434 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42756

Sequence Viewer

Length: 2865 bp
ATGGCCAGCCTCGCATTCGGTTCCAGCCTCACTTTACTTCGCGCCAACCAAAACGACGCCGCACAGAATAAGCCTCTTATCTACACTCCCAATTTCATCTCTCTCCGATTCAACCACAGCCTCAGAACCCACGCTTCGTCGTCGACGCGGTGCCGCGGCGTCGGATCTCCACGCGCCGCCGCGGAGGTGGTGGAGGATGTAACTGTCGAGGGAGAGGAGAAGGAGAATCGTAGGGTTCTGAGAGTCGGGGTCATCTGCGGTGGCCCCTCCGCCGAGCGTGGAATCTCTCTCAACTCCGCTAGATCAGTAATCGATCATATTCAAGGAGAGGATTTACATGTGAGTTGCTACTACATTGACTCTGAACTCAACGCTTTTGCAATTTCCCCTGCTCAGGTGTACTCCAACACTCCGGCGGATTTTGATTTCAAACTCGAAAGCCTTGCTCAAGGTTTCAAGTCTTGGTCTGACTTTGCGGACCATCTTGCTGTCAATGTGGACATAGTGTTTCCAGTCATACATGGTCAATTTGGTGAAGATGGCGGCATTCAGGAGGTGTTGGAAAGGTATAACATTCCATTTGTTGGCACGGGATCAAATGAGTGTCGTCAAGCATTCGACAAGTACAATGCCTCATTGGAGCTCAGCAGACATGGATTCGTAACTGTACCAAGTTGTTTAGTACAGGGAAGTAAAGCAGATGAACCTGAACTGTCAGAATGGTTTGCAAAAAATCAACTGGATCCTAAGTCCGGAAAAGTTGTGGTAAAACCAGCACGAGCAGGTTCAAGCATTGGTGTTACAGTTGCATATGGCTTGGCTGATTCTCTTAGCAAGGCTAATGCAATTATTACAGAGGGAATTGATTCCAAAGTCCTTGTTGAAATATTTCTTGAAGGAGGGAGTGAATTTACAGCAATTGTTCTTGATGTGGGGTATGGTACAGATTCTCATCCTGTTGTACTATTACCAACTGAGGTGGAACTTCAATTCCTTGGTAGTGTAGATGTTAGAGAGAAGGATGCAATCTTCAACTACCGCAGGAAATATCTTCCAACACAACAGGTTGCTTATCATACTCCACCTCGTTTTCCTATTGATGTCATCCAAAATATTCGAGATGGAGCATCTCAGTTATTCCAAAAGCTTGGCCTTCGTGACTTTGCTCGAATCGATGGATGGTTTCTGCCTAATTCTATTCATGTACCATCATCGCCAGATAGCAAGTTTGGAAGGACTGGGATGGGCACAATATTATACACTGACATTAACCTGATTAGTGGTATGGAGCAGACCAGCTTTTTATTCCAGCAAGCCTCAAAGGTTGGGTTTTCTCATGCAAATATTCTCCGGAGCATCATTAATCATGCTTGTTTGCGATTTCCGCATCTTGCATCTTGTGATGTGTCTAGTGATTTGGCTAGAACATCAAAATCCCCACTGCTTAAAGATGATTGGGAAGGCACTCAGAAAGTTTTTGTTATTTTTGGAGGAGACACATCAGAGAGGCAAGTATCTCTTATGAGTGGAACAAATGTTTGGCTCAATTTGCAAGCTTTTGATGATCTGGAAGTGCTTCCTTGCTTGCTTGCCCCCACGAATGGATATTCGTCTAGTACTGATGTGGACAAAAATGAAGTTGATGCAACTTCCCAAACAGTTTGGTCATTACCTTATTCTCTTGTGCTAAGACACACCACGGAGGAAGTTCTTGCTGCATGTGTAGAGGCAATAGAACCAGACCGAGCTGCACTTACATCTCAATTACGGAACAGAGTTATTGATGATCTTATGGAAGGCTTCAAGAAACATTCTTGGTTTACAGGATTTGATATCACTGATGAACTGCCTGTTAAATTTTCTATTGAAGAGTGGATAAAGCTAGCCAAGGAAGTCAAGGCAACAGTATTTATTGCAGTGCATGGAGGCATTGGTGAAGATGGTACACTTCAGTCTTTGTTGGAGGCTGAAGGGATTCCACATACAGGTCCTGGTGTTTTGGCTTCAAAGATTTGTATGGACAAAGTCGCAACATCAGTTGCTCTTAAACATCTATCGGACTTGGGAGTTCTTACCATAAACAAAGATTTGAGAAGAAGAGATCAGCTCCTTAATACACCAATACCAAATGTATGGCATGAATTGACCTCCAAGCTTCAGTGTGAAACATTATGTGTTAAACCGGCAAGAGATGGATGCTCAACTGGAGTTGCAAGGTTATGCTGTGATGAAGACCTTTCAGTGTATGTAAAAGCATTGGAGGACTGTCTCCTTCGGATTCCCCCTAATAGTTTGTCAAAGGAACACGGAATGATCGAAATGCCAAACCCCCCTCCAGAGCTCCTAATCTTTGAACCTTTTATTGATACGGATGACATAATTGTTTCATTGAAGTCCATAAATGAAAATGGACATCACCTCATGTGGAATGGACAGAGTAGATGGGTTGAAATAACTGTTGGTGTTATTGGGAAACAAGGATCAATGCACTCATTGAGTCCTAGTATCACTGTGAAGGAGAGTGGTGATATTTTATCACTTGAGGAGAAATTTCAGGGTGGGACTGGCATCAATCTTACTCCACCTCCGTCATCGATCATTAGCCACGAGGCCTTGCAGAAATGTAAACAACACATTGAAATGATTGCCAACACTTTAGAATTAGAAGGATTTTCACGAATTGATGCCTTTGTCAACGTGGATAGTGGAGAGGTGCTGGTTATAGAGGTCAATACAGTGCCTGGAATGACACCTTCCACTGTTCTGATTCATCAGGCACTAGCAGAGGTACCGCCCATGTATCCTCACCAGTTCTTCCGGACACTGCTTGATTTGGCATCAGAGAGGACAATATACGTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dala_Dala_lig_C Dala_Dala_lig_N

Protein Analysis

954

Amino Acids

104.69

Weight (kDa)

5.16

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dala_Dala_lig_N PF01820 81 - 196 2.2e-24 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 254 - 392 3.5e-13 D-ala D-ala ligase C-terminus
Dala_Dala_lig_N PF01820 491 - 664 1.1e-17 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 816 - 922 1.5e-11 D-ala D-ala ligase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 773
Acc65I GGTACC 1 cut(s) 2788
AccB1I GGYRCC 2 cut(s) 150, 2788
AccB7I CCANNNNNTGG 1 cut(s) 584
AccI GTMKAC 1 cut(s) 143
AccII CGCG 5 cut(s) 42, 148, 156, 174, 182
AccIII TCCGGA 3 cut(s) 752, 1350, 2817
AclWI GGATC 5 cut(s) 172, 601, 737, 750, 2488
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 908, 1856, 2549
AcuI CTGAAG 3 cut(s) 1934, 1989, 2141
AcyI GRCGYC 2 cut(s) 57, 159
AfiI CCNNNNNNNGG 5 cut(s) 394, 584, 752, 1601, 1985
AflIII ACRYGT 1 cut(s) 337
AjnI CCWGG 2 cut(s) 1990, 2740
AjuI GAANNNNNNNTTGG 4 cut(s) 1522, 1554, 2442, 2474
AloI GAACNNNNNNTCC 2 cut(s) 975, 1007
AluBI AGCT 9 cut(s) 643, 1147, 1299, 1556, 1748, 1882, 2107, 2155, 2341
AluI AGCT 9 cut(s) 643, 1147, 1299, 1556, 1748, 1882, 2107, 2155, 2341
Alw21I GWGCWC 2 cut(s) 645, 2343
Alw26I GTCTC 2 cut(s) 1488, 2273
AlwI GGATC 5 cut(s) 172, 601, 737, 750, 2488
AlwNI CAGNNNCTG 2 cut(s) 1991, 2741
Aor13HI TCCGGA 3 cut(s) 752, 1350, 2817
AoxI GGCC 4 cut(s) 3, 262, 1150, 2610
ApeKI GCWGC 2 cut(s) 1715, 1748
ApoI RAATTY 3 cut(s) 908, 1856, 2549
AseI ATTAAT 1 cut(s) 1362
Asp700I GAANNNNTTC 4 cut(s) 865, 888, 1575, 1974
Asp718I GGTACC 1 cut(s) 2788
AspLEI GCGC 2 cut(s) 44, 176
AspS9I GGNCC 3 cut(s) 263, 478, 1988
AsuHPI GGTGA 5 cut(s) 545, 1946, 2408, 2537, 2798
AsuNHI GCTAGC 1 cut(s) 1882
AvaII GGWCC 2 cut(s) 478, 1988
BaeGI GKGCMC 1 cut(s) 1250
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 742
BanI GGYRCC 2 cut(s) 150, 2788
BanII GRGCYC 2 cut(s) 645, 2343
BauI CACGAG 2 cut(s) 777, 2606
BbsI GAAGAC 1 cut(s) 2238
Bbv12I GWGCWC 2 cut(s) 645, 2343
BbvI GCAGC 2 cut(s) 1702, 1735
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 2 cut(s) 1992, 2742
BciVI GTATCC 1 cut(s) 2811
BcoDI GTCTC 2 cut(s) 1488, 2273
BfaI CTAG 7 cut(s) 300, 1410, 1422, 1614, 1883, 2502, 2780
BfuAI ACCTGC 1 cut(s) 773
BfuI GTATCC 1 cut(s) 2811
BisI GCNGC 8 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749
BlpI GCTNAGC 1 cut(s) 644
BlsI GCNGC 8 cut(s) 61, 155, 158, 178, 181, 545, 1717, 1750
BmcAI AGTACT 1 cut(s) 1618
Bme1390I CCNGG 2 cut(s) 1992, 2742
Bme18I GGWCC 2 cut(s) 478, 1988
BmgT120I GGNCC 3 cut(s) 263, 478, 1988
BmiI GGNNCC 5 cut(s) 22, 152, 265, 744, 2790
BmrFI CCNGG 2 cut(s) 1992, 2742
BmrI ACTGGG 1 cut(s) 1248
BmtI GCTAGC 1 cut(s) 1886
BmuI ACTGGG 1 cut(s) 1248
BpiI GAAGAC 1 cut(s) 2238
BplI GAGNNNNNCTC 2 cut(s) 2091, 2123
BpmI CTGGAG 2 cut(s) 2226, 2319
Bpu10I CCTNAGC 1 cut(s) 393
Bpu1102I GCTNAGC 1 cut(s) 644
BpuEI CTTGAG 2 cut(s) 432, 2561
Bsa29I ATCGAT 3 cut(s) 312, 1173, 2594
BsaBI GATNNNNATC 1 cut(s) 951
BsaHI GRCGYC 2 cut(s) 57, 159
BsaJI CCNNGG 5 cut(s) 154, 180, 994, 1698, 1887
BsaWI WCCGGW 3 cut(s) 752, 1350, 2817
BsaXI ACNNNNNCTCC 2 cut(s) 2569, 2599
Bsc4I CCNNNNNNNGG 5 cut(s) 394, 584, 752, 1601, 1985
Bse118I RCCGGY 1 cut(s) 2182
Bse1I ACTGG 6 cut(s) 512, 744, 1243, 2209, 2569, 2809
Bse8I GATNNNNATC 1 cut(s) 951
BseAI TCCGGA 3 cut(s) 752, 1350, 2817
BseBI CCWGG 2 cut(s) 1992, 2742
BseCI ATCGAT 3 cut(s) 312, 1173, 2594
BseDI CCNNGG 5 cut(s) 154, 180, 994, 1698, 1887
BseGI GGATG 8 cut(s) 202, 952, 1027, 1104, 1184, 1248, 2201, 2377
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 5 cut(s) 394, 584, 752, 1601, 1985
BseMII CTCAG 7 cut(s) 136, 230, 407, 658, 966, 1145, 1481
BseNI ACTGG 6 cut(s) 512, 744, 1243, 2209, 2569, 2809
BseRI GAGGAG 3 cut(s) 230, 1506, 2558
BseSI GKGCMC 1 cut(s) 1250
BseXI GCAGC 2 cut(s) 1702, 1735
BsgI GTGCAG 1 cut(s) 1734
Bsh1236I CGCG 5 cut(s) 42, 148, 156, 174, 182
BshFI GGCC 4 cut(s) 5, 264, 1152, 2612
BshNI GGYRCC 2 cut(s) 150, 2788
BshVI ATCGAT 3 cut(s) 312, 1173, 2594
BsiHKAI GWGCWC 2 cut(s) 645, 2343
BsiSI CCGG 5 cut(s) 413, 753, 1351, 2183, 2818
BslFI GGGAC 1 cut(s) 2575
BslI CCNNNNNNNGG 5 cut(s) 394, 584, 752, 1601, 1985
BsmAI GTCTC 2 cut(s) 1488, 2273
BsmFI GGGAC 1 cut(s) 2575
BsmI GAATGC 3 cut(s) 14, 546, 614
BsnI GGCC 4 cut(s) 5, 264, 1152, 2612
Bsp1286I GDGCHC 3 cut(s) 645, 1250, 2343
Bsp13I TCCGGA 3 cut(s) 752, 1350, 2817
Bsp1720I GCTNAGC 1 cut(s) 644
BspANI GGCC 4 cut(s) 5, 264, 1152, 2612
BspCNI CTCAG 7 cut(s) 135, 231, 406, 657, 967, 1144, 1480
BspDI ATCGAT 3 cut(s) 312, 1173, 2594
BspEI TCCGGA 3 cut(s) 752, 1350, 2817
BspFNI CGCG 5 cut(s) 42, 148, 156, 174, 182
BspLI GGNNCC 5 cut(s) 22, 152, 265, 744, 2790
BspMI ACCTGC 1 cut(s) 773
BspOI GCTAGC 1 cut(s) 1886
BspPI GGATC 5 cut(s) 172, 601, 737, 750, 2488
BspT107I GGYRCC 2 cut(s) 150, 2788
BsrFI RCCGGY 1 cut(s) 2182
BsrI ACTGG 6 cut(s) 512, 744, 1243, 2209, 2569, 2809
BssAI RCCGGY 1 cut(s) 2182
BssECI CCNNGG 5 cut(s) 154, 180, 994, 1698, 1887
BssNI GRCGYC 2 cut(s) 57, 159
BssSI CACGAG 2 cut(s) 777, 2606
BssT1I CCWWGG 2 cut(s) 994, 1887
Bst2BI CACGAG 2 cut(s) 777, 2606
Bst2UI CCWGG 2 cut(s) 1992, 2742
Bst6I CTCTTC 2 cut(s) 1863, 2092
BstACI GRCGYC 2 cut(s) 57, 159
BstC8I GCNNGC 6 cut(s) 7, 1314, 1554, 1586, 1590, 1884
BstDSI CCRYGG 3 cut(s) 154, 180, 1698
BstF5I GGATG 8 cut(s) 202, 952, 1027, 1104, 1184, 1248, 2201, 2377
BstFNI CGCG 5 cut(s) 42, 148, 156, 174, 182
BstHHI GCGC 2 cut(s) 44, 176
BstMAI GTCTC 2 cut(s) 1488, 2273
BstMWI GCNNNNNNNGC 3 cut(s) 11, 1384, 1549
BstNI CCWGG 2 cut(s) 1992, 2742
BstNSI RCATGY 2 cut(s) 341, 1722
BstSCI CCNGG 2 cut(s) 1990, 2740
BstSLI GKGCMC 1 cut(s) 1250
BstUI CGCG 5 cut(s) 42, 148, 156, 174, 182
BstV1I GCAGC 2 cut(s) 1702, 1735
BstV2I GAAGAC 1 cut(s) 2238
BstX2I RGATCY 2 cut(s) 164, 742
BstXI CCANNNNNNTGG 2 cut(s) 1148, 2133
BstYI RGATCY 2 cut(s) 164, 742
Bsu15I ATCGAT 3 cut(s) 312, 1173, 2594
BsuI GTATCC 1 cut(s) 2811
BsuRI GGCC 4 cut(s) 5, 264, 1152, 2612
BsuTUI ATCGAT 3 cut(s) 312, 1173, 2594
BtgI CCRYGG 3 cut(s) 154, 180, 1698
BtgZI GCGATG 1 cut(s) 1197
BtsCI GGATG 8 cut(s) 202, 952, 1027, 1104, 1184, 1248, 2201, 2377
BtsI GCAGTG 3 cut(s) 1439, 1923, 2822
BveI ACCTGC 1 cut(s) 773
Cac8I GCNNGC 6 cut(s) 7, 1314, 1554, 1586, 1590, 1884
CaiI CAGNNNCTG 2 cut(s) 1991, 2741
CfoI GCGC 2 cut(s) 44, 176
Cfr10I RCCGGY 1 cut(s) 2182
Cfr13I GGNCC 3 cut(s) 263, 478, 1988
Cfr42I CCGCGG 2 cut(s) 157, 183
ClaI ATCGAT 3 cut(s) 312, 1173, 2594
CseI GACGC 3 cut(s) 65, 148, 154
CspCI CAANNNNNGTGG 2 cut(s) 960, 995
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 1863, 2092
EarI CTCTTC 2 cut(s) 1863, 2092
EciI GGCGGA 2 cut(s) 259, 431
Ecl136II GAGCTC 2 cut(s) 643, 2341
Eco130I CCWWGG 2 cut(s) 994, 1887
Eco147I AGGCCT 1 cut(s) 2612
Eco24I GRGCYC 2 cut(s) 645, 2343
Eco32I GATATC 1 cut(s) 1834
Eco47I GGWCC 2 cut(s) 478, 1988
Eco53kI GAGCTC 2 cut(s) 643, 2341
Eco57I CTGAAG 3 cut(s) 1934, 1989, 2141
EcoICRI GAGCTC 2 cut(s) 643, 2341
EcoO109I RGGNCCY 1 cut(s) 1988
EcoRII CCWGG 2 cut(s) 1990, 2740
EcoRV GATATC 1 cut(s) 1834
EcoT14I CCWWGG 2 cut(s) 994, 1887
EcoT38I GRGCYC 2 cut(s) 645, 2343
ErhI CCWWGG 2 cut(s) 994, 1887
FalI AAGNNNNNCTT 2 cut(s) 1503, 1535
FaqI GGGAC 1 cut(s) 2575
FauNDI CATATG 1 cut(s) 811
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 8 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749
FokI GGATG 8 cut(s) 209, 939, 1034, 1091, 1191, 1255, 2208, 2384
FriOI GRGCYC 2 cut(s) 645, 2343
Fsp4HI GCNGC 8 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749
FspBI CTAG 7 cut(s) 300, 1410, 1422, 1614, 1883, 2502, 2780
GlaI GCGC 2 cut(s) 43, 175
GluI GCNGC 8 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749
GsuI CTGGAG 2 cut(s) 2226, 2319
HaeIII GGCC 4 cut(s) 5, 264, 1152, 2612
HapII CCGG 5 cut(s) 413, 753, 1351, 2183, 2818
HgaI GACGC 3 cut(s) 65, 148, 154
HhaI GCGC 2 cut(s) 44, 176
Hin1I GRCGYC 2 cut(s) 57, 159
Hin6I GCGC 2 cut(s) 42, 174
HinP1I GCGC 2 cut(s) 42, 174
HincII GTYRAC 2 cut(s) 144, 2695
HindII GTYRAC 2 cut(s) 144, 2695
HindIII AAGCTT 3 cut(s) 1145, 1554, 2153
HpaII CCGG 5 cut(s) 413, 753, 1351, 2183, 2818
HphI GGTGA 5 cut(s) 545, 1946, 2408, 2537, 2798
Hpy166II GTNNAC 8 cut(s) 144, 400, 499, 1627, 1821, 1946, 2627, 2695
Hpy8I GTNNAC 8 cut(s) 144, 400, 499, 1627, 1821, 1946, 2627, 2695
Hpy99I CGWCG 5 cut(s) 59, 142, 145, 148, 164
HpyCH4IV ACGT 2 cut(s) 2697, 2856
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 1384, 1549
HpySE526I ACGT 2 cut(s) 2697, 2856
Hsp92I GRCGYC 2 cut(s) 57, 159
HspAI GCGC 2 cut(s) 42, 174
Kpn2I TCCGGA 3 cut(s) 752, 1350, 2817
KpnI GGTACC 1 cut(s) 2792
KspI CCGCGG 2 cut(s) 157, 183
LmnI GCTCC 6 cut(s) 640, 1124, 1288, 1353, 2112, 2346
Lsp1109I GCAGC 2 cut(s) 1702, 1735
MaeI CTAG 7 cut(s) 300, 1410, 1422, 1614, 1883, 2502, 2780
MaeII ACGT 2 cut(s) 2697, 2856
MaeIII GTNAC 4 cut(s) 199, 661, 799, 1157
MboII GAAGA 9 cut(s) 548, 1021, 1043, 1880, 1949, 2106, 2109, 2243, 2806
MfeI CAATTG 1 cut(s) 918
MflI RGATCY 2 cut(s) 164, 742
MhlI GDGCHC 3 cut(s) 645, 1250, 2343
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 252, 353, 2506
MmeI TCCRAC 5 cut(s) 142, 429, 540, 1079, 1941
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 3 cut(s) 752, 1350, 2817
MroXI GAANNNNTTC 4 cut(s) 865, 888, 1575, 1974
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 1269, 1362, 1446, 1854, 2046, 2112, 2178
MslI CAYNNNNRTG 1 cut(s) 2639
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 156, 182
MspI CCGG 5 cut(s) 413, 753, 1351, 2183, 2818
MspR9I CCNGG 2 cut(s) 1992, 2742
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 3 cut(s) 14, 546, 614
MvaI CCWGG 2 cut(s) 1992, 2742
MvnI CGCG 5 cut(s) 42, 148, 156, 174, 182
MwoI GCNNNNNNNGC 3 cut(s) 11, 1384, 1549
NdeI CATATG 1 cut(s) 811
NheI GCTAGC 1 cut(s) 1882
NlaIV GGNNCC 5 cut(s) 22, 152, 265, 744, 2790
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 1157
NspI RCATGY 2 cut(s) 341, 1722
PceI AGGCCT 1 cut(s) 2612
PciI ACATGT 1 cut(s) 337
PcsI WCGNNNNNNNCGW 1 cut(s) 2313
PctI GAATGC 3 cut(s) 14, 546, 614
PdmI GAANNNNTTC 4 cut(s) 865, 888, 1575, 1974
PflFI GACNNNGTC 1 cut(s) 2024
PflMI CCANNNNNTGG 1 cut(s) 584
PkrI GCNGC 8 cut(s) 61, 155, 158, 178, 181, 545, 1717, 1750
PleI GAGTC 3 cut(s) 251, 353, 2505
PpsI GAGTC 3 cut(s) 251, 353, 2505
PpuMI RGGWCCY 1 cut(s) 1988
PscI ACATGT 1 cut(s) 337
PshBI ATTAAT 1 cut(s) 1362
Psp124BI GAGCTC 2 cut(s) 645, 2343
Psp5II RGGWCCY 1 cut(s) 1988
Psp6I CCWGG 2 cut(s) 1990, 2740
PspGI CCWGG 2 cut(s) 1990, 2740
PspN4I GGNNCC 5 cut(s) 22, 152, 265, 744, 2790
PspPI GGNCC 3 cut(s) 263, 478, 1988
PspPPI RGGWCCY 1 cut(s) 1988
PstNI CAGNNNCTG 2 cut(s) 1991, 2741
PsuI RGATCY 2 cut(s) 164, 742
PsyI GACNNNGTC 1 cut(s) 2024
RseI CAYNNNNRTG 1 cut(s) 2639
SacI GAGCTC 2 cut(s) 645, 2343
SacII CCGCGG 2 cut(s) 157, 183
SalI GTCGAC 1 cut(s) 142
SaqAI TTAA 7 cut(s) 1269, 1362, 1446, 1854, 2046, 2112, 2178
SatI GCNGC 8 cut(s) 60, 154, 157, 177, 180, 544, 1716, 1749
Sau96I GGNCC 3 cut(s) 263, 478, 1988
ScaI AGTACT 1 cut(s) 1618
SchI GAGTC 3 cut(s) 252, 353, 2506
ScrFI CCNGG 2 cut(s) 1992, 2742
SduI GDGCHC 3 cut(s) 645, 1250, 2343
Sfr303I CCGCGG 2 cut(s) 157, 183
SgrBI CCGCGG 2 cut(s) 157, 183
SgrDI CGTCGACG 1 cut(s) 142
SinI GGWCC 2 cut(s) 478, 1988
SmiMI CAYNNNNRTG 1 cut(s) 2639
SmlI CTYRAG 2 cut(s) 447, 2540
SmoI CTYRAG 2 cut(s) 447, 2540
SseBI AGGCCT 1 cut(s) 2612
SspI AATATT 4 cut(s) 888, 1114, 1254, 1345
SspMI CTAG 7 cut(s) 300, 1410, 1422, 1614, 1883, 2502, 2780
SstI GAGCTC 2 cut(s) 645, 2343
StuI AGGCCT 1 cut(s) 2612
StyD4I CCNGG 2 cut(s) 1990, 2740
StyI CCWWGG 2 cut(s) 994, 1887
TaiI ACGT 2 cut(s) 2700, 2859
TaqII GACCGA 1 cut(s) 1758
TatI WGTACW 5 cut(s) 399, 624, 682, 961, 1616
TauI GCSGC 6 cut(s) 62, 156, 159, 179, 182, 546
Tru1I TTAA 7 cut(s) 1269, 1362, 1446, 1854, 2046, 2112, 2178
Tru9I TTAA 7 cut(s) 1269, 1362, 1446, 1854, 2046, 2112, 2178
TseFI GTSAC 1 cut(s) 1157
TseI GCWGC 2 cut(s) 1715, 1748
Tsp45I GTSAC 1 cut(s) 1157
TspGWI ACGGA 5 cut(s) 1715, 1783, 2322, 2384, 2577
Tth111I GACNNNGTC 1 cut(s) 2024
Van91I CCANNNNNTGG 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 478, 1988
VspI ATTAAT 1 cut(s) 1362
XapI RAATTY 3 cut(s) 908, 1856, 2549
XceI RCATGY 2 cut(s) 341, 1722
XmiI GTMKAC 1 cut(s) 143
XmnI GAANNNNTTC 4 cut(s) 865, 888, 1575, 1974
XspI CTAG 7 cut(s) 300, 1410, 1422, 1614, 1883, 2502, 2780
ZrmI AGTACT 1 cut(s) 1618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.