MD04G1168900.v1.1

D-ala D-ala ligase N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005073
Physical Location & Seq
Reverse (-)
25930927 .. 25938295
7369 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1168900.v1.1.491

Sequence Viewer

Length: 2835 bp
ATGGCGACGATCGCATTTGGTTCCAGCCTTGTTTCAGTTCGCGCCGAGCAAACCGACGCTGTTCGGAGCTCGCTGCTGTACACCCAGAACTTCAATTTCTTGCGGTTGAAGAGAAGCTGTGCTTCGTCGATGACGCGGTGGCGCGGCGTCGGAGTTCCACGCGCCACTGCGGAGGCGGTGGGCGGTGGAGTTATTGAGAGGGGAGAGGAGGTGAAAGAGAAGGACAAAGGCAGCGTTCTGAGAGTTGGCGTCGTTTGCGGAGGTCCCTCGGCGGAGCGTGGGATTTCTCTCAACTCGGCTCGATCAGTTCTCGATCACATTCAGGGAGGTGATATACATGTGAATTGCTATTACCTTGACGCTGGCCTCAATGCATTTGCAATATCCCCTGCTCAGGTTTACTCAAACACTCCCGCGGATTTTGATTTTAAACTCGAAAGCCTTGCCCAAGGTTTCCGGTCTTTGTCTGACTTCGCTGAACATCTAGCTGGCTCTGTGGACATAGTGTTCCCAGTCATACACGGTCAATTTGGTGAAGACGGTGGCATTCAGCTGCTGGAAAAGTATAACGTTCCATTTGTTGGCACGGGATCAAGTGAATGTTGCCAAGCATTCGATAAGTACAATGCTTCGTTGGAGCTCAGCAAACAAGGATTTATAACAGTACCAAGTTGTTTAGTACAGGGAAGTGAAGCAGATGAATCTGAACTGTCAAAATGGTTTGCAAGAAATCAACTGGATGTTGAGTCAGGGAAAGTTGTGGTAAAACCAGCACGAGCGGGATCAAGCATTGGTGTTACAGTTGCATATGGTGTGGCTGATACTTTTAGCAAGGCTAATGCAATTATTACAGAGGGAATTGACTCCAAAGTCCTTGTAGAAATATTTCTTGAAGGAGGGAGTGAGTTTACAGCAATTGTTCTTGATGTGGGGTCTGGATTAGATTCCCATCCTGTTGTATTACTACCAAGCGAGGTAGAACTTCAATTCCATGACAGTGTTGATAAGGATGCAATTTTCAACTACAGGAGGAAATATCTTCCAACACAACAGGTTGCTTATCATACCCCACCTCGTTTTCCGATTGGTGTTATCGAAAGTATTCGAGAAGGAGCATCTCAGTTATTCAGAAAGCTTGGTCTTCGTGACTTTGCTCGTATTGATGGATGGTTTCTCCCTAATTCTATTCATGTACCAACATTGTCAGATAGCAAATTCGGAAGGACTGAGATGGGTACAATATTCTATACTGACATTAATCTGATTAGTGGGATGGAGCAGACCAGCTTTTTATTTCAGCAAGCCTCGAAGGTTGGATTTTCTCATTCAAATATTCTCCGGAGCATAATCCGGCGTGCTTGTATGCGGTATCCAAACCTTGCACCATTTTGTAGTGCATCAGATCATGTTCCTAGAAGATCAAAAACTTCACTGCGTAGTGAACCAGTTTGCAAGGGTAAGCGCAAAGTTTTTGTTATTTTTGGAGGAGACTCATCAGAGAGACAGGTATCTCTTATCAGCGGAACAAACGTTTGGCTTAATTTGCAAGCTTTTGACGATCTTGAAGTGATTCCATGTTTGCTTGCCCCCACAATTGTCAAAAACAAGGTTGATTTGAGTTCCAGAACCGTTTGGTCTTTACCTTACTCTCTTGTTCTGAGACACACCATAGAGGAAGTTCTTGATGCATGCGTAGAGGCAATTGAACCAGACCGAGTTGCTCATACATCTCACTTAAGGGACAGAGTTGTTCACGATCTTACAGAAGGCTTGAAGAAACATTCTTGGTTCACGGGGTTTGATATCACTGACGAACTGCCCGTAAAATTATCGGTTGAACAGTGGATCAAGCTAGCCAAGGAAGTTCAGGCTACAGTTTTTCTTTCAGTGCATGGAGGCATTGGTGAAGATGGCACACTTCAGTCTTTGTTGGAGGTTGAAGGGATTCCACATACAGGTCCTGGGGTGATGGCATCAAAGATTTGTATGGACAAACTTGCAACGTCCCTTGCTCTTAATCATCTGTCGGATTTGGGAGTTCTTACTATCAACAAAGATTTGAGGAGAAAAGAAGATCTCATTAGTTCATCAATACCAAATCTATGGCATGAATTGACCTCAAAGCTTCACTGTGAAACATTATGTGTTAAACCTGCAAGAGATGGATGCTCAACGGGGGTTGGAAGGTTATGCTGCGCTGATGACCTTTTAGTGTATGTAAAAGCATTGGAAGACTGTCTTCACCGGATTCCTCCTAATAGTTTCTCTAAGGCGCATGGAACAATTGAGATGCCAAACCCCCCTCCAGAGCTCTTAATCTTCGAGCCTTTTATCGAGACCGATAATATAATTGTTTCATCAAAATCTAGAAATGAAAAGGGGCATCAGATCTTGTGGAGCGGAGAGAGTAGATGGGTTGAAATAACTGTCGGAGTTGTTGGAACACAAGGATCTATGCGCTCGCTGACTCCTAGCATCACGGTCAGGGAGAGTGGTGACATTTTAACACTTGAGGAGAAATTCCAAGGTGGGACTGGCATCAATCTGACTCCACCTCCATCATCTATTATTAGCAACGAGGCCTTGCAGAAATGTAAAAAACATATCGAAATAATTGCAAATAGTCTTGAATTAGAAGGATTTTCACGGATTGATGCATTTGTCAACGTGGACAGTGGAGAGGTCTTGATGATAGAGGTCAACACAGTGCCTGGAATGACGCCTTCGACTGTCCTGATTCATCAGGCGCTAGCCGAGGAACCTCCCTTGTACCCTCACGAGTTTTTCCGGACGCTGCTTGATTTAGCATTAGAGAGGACCGTTTATACGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dala_Dala_lig_C Dala_Dala_lig_N

Protein Analysis

945

Amino Acids

103.41

Weight (kDa)

5.64

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dala_Dala_lig_N PF01820 81 - 195 2.2e-20 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 252 - 450 1.1e-15 D-ala D-ala ligase C-terminus
Dala_Dala_lig_N PF01820 489 - 654 1.1e-17 D-ala D-ala ligase N-terminus
Dala_Dala_lig_C PF07478 806 - 912 8.3e-11 D-ala D-ala ligase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 659
AasI GACNNNNNNGTC 1 cut(s) 869
Acc36I ACCTGC 1 cut(s) 2161
AccB7I CCANNNNNTGG 1 cut(s) 581
AccBSI CCGCTC 2 cut(s) 779, 2400
AccII CGCG 5 cut(s) 42, 136, 144, 162, 416
AccIII TCCGGA 2 cut(s) 1338, 2787
AclI AACGTT 2 cut(s) 570, 1530
AclWI GGATC 4 cut(s) 598, 790, 1853, 2458
AcsI RAATTY 2 cut(s) 1214, 2519
AcuI CTGAAG 1 cut(s) 1904
AcyI GRCGYC 3 cut(s) 147, 249, 2720
AfaI GTAC 7 cut(s) 80, 623, 666, 681, 1194, 1237, 2771
AfiI CCNNNNNNNGG 3 cut(s) 394, 581, 1955
AflII CTTAAG 1 cut(s) 1735
AflIII ACRYGT 1 cut(s) 337
AjnI CCWGG 2 cut(s) 1960, 2710
AjuI GAANNNNNNNTTGG 2 cut(s) 1516, 1548
AloI GAACNNNNNNTCC 4 cut(s) 491, 523, 972, 1004
Alw21I GWGCWC 3 cut(s) 71, 642, 2313
Alw26I GTCTC 4 cut(s) 1482, 1495, 1654, 2330
AlwI GGATC 4 cut(s) 598, 790, 1853, 2458
AlwNI CAGNNNCTG 3 cut(s) 556, 1961, 2711
Aor13HI TCCGGA 2 cut(s) 1338, 2787
AoxI GGCC 2 cut(s) 364, 2580
ApeKI GCWGC 5 cut(s) 73, 231, 553, 2193, 2794
ApoI RAATTY 2 cut(s) 1214, 2519
AseI ATTAAT 1 cut(s) 1257
Asp700I GAANNNNTTC 4 cut(s) 885, 1101, 1569, 1944
AspLEI GCGC 8 cut(s) 44, 144, 164, 1464, 2198, 2275, 2460, 2749
AspS9I GGNCC 3 cut(s) 263, 1958, 2817
AsuHPI GGTGA 7 cut(s) 223, 341, 545, 1916, 1978, 2234, 2507
AsuNHI GCTAGC 2 cut(s) 1852, 2749
AvaII GGWCC 3 cut(s) 263, 1958, 2817
BanII GRGCYC 3 cut(s) 71, 642, 2313
BauI CACGAG 2 cut(s) 774, 2777
BbsI GAAGAC 4 cut(s) 543, 1133, 2231, 2238
Bbv12I GWGCWC 3 cut(s) 71, 642, 2313
BbvI GCAGC 5 cut(s) 60, 243, 540, 2180, 2781
BcgI CGANNNNNNTGC 4 cut(s) 425, 459, 2597, 2631
BciT130I CCWGG 2 cut(s) 1962, 2712
BciVI GTATCC 1 cut(s) 1380
BcoDI GTCTC 4 cut(s) 1482, 1495, 1654, 2330
BfaI CTAG 6 cut(s) 485, 1413, 1853, 2367, 2472, 2750
BfmI CTRYAG 2 cut(s) 1024, 1872
BfoI RGCGCY 1 cut(s) 2750
BfrI CTTAAG 1 cut(s) 1735
BfuAI ACCTGC 1 cut(s) 2161
BfuI GTATCC 1 cut(s) 1380
BglII AGATCT 2 cut(s) 2074, 2388
BisI GCNGC 6 cut(s) 74, 145, 232, 554, 2194, 2795
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 6 cut(s) 75, 146, 233, 555, 2195, 2796
Bme1390I CCNGG 2 cut(s) 1962, 2712
Bme18I GGWCC 3 cut(s) 263, 1958, 2817
BmgT120I GGNCC 3 cut(s) 263, 1958, 2817
BmiI GGNNCC 3 cut(s) 22, 265, 2760
BmrFI CCNGG 2 cut(s) 1962, 2712
BmrI ACTGGG 1 cut(s) 506
BmtI GCTAGC 2 cut(s) 1856, 2753
BmuI ACTGGG 1 cut(s) 506
BpiI GAAGAC 4 cut(s) 543, 1133, 2231, 2238
BpmI CTGGAG 1 cut(s) 2289
Bpu10I CCTNAGC 1 cut(s) 393
Bpu1102I GCTNAGC 1 cut(s) 641
BpuEI CTTGAG 1 cut(s) 2531
BsaBI GATNNNNATC 1 cut(s) 948
BsaHI GRCGYC 3 cut(s) 147, 249, 2720
BsaI GGTCTC 1 cut(s) 2330
BsaJI CCNNGG 7 cut(s) 267, 414, 448, 1857, 1961, 2524, 2754
BsaWI WCCGGW 4 cut(s) 456, 1338, 2244, 2787
BsaXI ACNNNNNCTCC 4 cut(s) 195, 225, 2539, 2569
Bsc4I CCNNNNNNNGG 3 cut(s) 394, 581, 1955
Bse1I ACTGG 4 cut(s) 512, 741, 1445, 2539
Bse8I GATNNNNATC 1 cut(s) 948
BseAI TCCGGA 2 cut(s) 1338, 2787
BseBI CCWGG 2 cut(s) 1962, 2712
BseDI CCNNGG 7 cut(s) 267, 414, 448, 1857, 1961, 2524, 2754
BseGI GGATG 6 cut(s) 745, 949, 1015, 1172, 1278, 2171
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 3 cut(s) 394, 581, 1955
BseMII CTCAG 6 cut(s) 230, 407, 655, 1133, 1218, 1649
BseNI ACTGG 4 cut(s) 512, 741, 1445, 2539
BseRI GAGGAG 4 cut(s) 221, 1500, 2077, 2528
BseXI GCAGC 5 cut(s) 60, 243, 540, 2180, 2781
Bsh1236I CGCG 5 cut(s) 42, 136, 144, 162, 416
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 366, 2582
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 3 cut(s) 71, 642, 2313
BsiSI CCGG 5 cut(s) 457, 1339, 1351, 2245, 2788
BslFI GGGAC 4 cut(s) 249, 1754, 1990, 2545
BslI CCNNNNNNNGG 3 cut(s) 394, 581, 1955
BsmAI GTCTC 4 cut(s) 1482, 1495, 1654, 2330
BsmFI GGGAC 4 cut(s) 249, 1754, 1990, 2545
BsmI GAATGC 2 cut(s) 546, 611
BsnI GGCC 2 cut(s) 366, 2582
Bso31I GGTCTC 1 cut(s) 2330
Bsp1286I GDGCHC 3 cut(s) 71, 642, 2313
Bsp13I TCCGGA 2 cut(s) 1338, 2787
Bsp1407I TGTACA 1 cut(s) 78
Bsp1720I GCTNAGC 1 cut(s) 641
BspANI GGCC 2 cut(s) 366, 2582
BspCNI CTCAG 6 cut(s) 231, 406, 654, 1132, 1219, 1650
BspEI TCCGGA 2 cut(s) 1338, 2787
BspFNI CGCG 5 cut(s) 42, 136, 144, 162, 416
BspLI GGNNCC 3 cut(s) 22, 265, 2760
BspMI ACCTGC 1 cut(s) 2161
BspOI GCTAGC 2 cut(s) 1856, 2753
BspPI GGATC 4 cut(s) 598, 790, 1853, 2458
BspTI CTTAAG 1 cut(s) 1735
BspTNI GGTCTC 1 cut(s) 2330
BsrBI CCGCTC 2 cut(s) 779, 2400
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 4 cut(s) 512, 741, 1445, 2539
BssECI CCNNGG 7 cut(s) 267, 414, 448, 1857, 1961, 2524, 2754
BssNI GRCGYC 3 cut(s) 147, 249, 2720
BssSI CACGAG 2 cut(s) 774, 2777
BssT1I CCWWGG 3 cut(s) 448, 1857, 2524
Bst2BI CACGAG 2 cut(s) 774, 2777
Bst2UI CCWGG 2 cut(s) 1962, 2712
Bst6I CTCTTC 1 cut(s) 104
BstACI GRCGYC 3 cut(s) 147, 249, 2720
BstAFI CTTAAG 1 cut(s) 1735
BstAUI TGTACA 1 cut(s) 78
BstDEI CTNAG 8 cut(s) 239, 393, 641, 1119, 1227, 1658, 2268, 2832
BstDSI CCRYGG 1 cut(s) 414
BstF5I GGATG 6 cut(s) 745, 949, 1015, 1172, 1278, 2171
BstFNI CGCG 5 cut(s) 42, 136, 144, 162, 416
BstH2I RGCGCY 1 cut(s) 2750
BstHHI GCGC 8 cut(s) 44, 144, 164, 1464, 2198, 2275, 2460, 2749
BstMAI GTCTC 4 cut(s) 1482, 1495, 1654, 2330
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 11, 255, 1543
BstNI CCWGG 2 cut(s) 1962, 2712
BstNSI RCATGY 2 cut(s) 341, 1692
BstSCI CCNGG 2 cut(s) 1960, 2710
BstSFI CTRYAG 2 cut(s) 1024, 1872
BstUI CGCG 5 cut(s) 42, 136, 144, 162, 416
BstV1I GCAGC 5 cut(s) 60, 243, 540, 2180, 2781
BstV2I GAAGAC 4 cut(s) 543, 1133, 2231, 2238
BstX2I RGATCY 3 cut(s) 2074, 2388, 2450
BstXI CCANNNNNNTGG 1 cut(s) 2103
BstYI RGATCY 3 cut(s) 2074, 2388, 2450
BsuI GTATCC 1 cut(s) 1380
BsuRI GGCC 2 cut(s) 366, 2582
BtgI CCRYGG 1 cut(s) 414
BtsCI GGATG 6 cut(s) 745, 949, 1015, 1172, 1278, 2171
BtsI GCAGTG 2 cut(s) 165, 1430
BtsIMutI CAGTG 9 cut(s) 165, 1003, 1430, 1806, 1847, 1893, 2128, 2680, 2712
BveI ACCTGC 1 cut(s) 2161
CaiI CAGNNNCTG 3 cut(s) 556, 1961, 2711
CfoI GCGC 8 cut(s) 44, 144, 164, 1464, 2198, 2275, 2460, 2749
Cfr13I GGNCC 3 cut(s) 263, 1958, 2817
Cfr42I CCGCGG 1 cut(s) 417
CseI GACGC 7 cut(s) 65, 136, 142, 238, 368, 2728, 2800
Csp6I GTAC 7 cut(s) 79, 622, 665, 680, 1193, 1236, 2770
CviAII CATG 9 cut(s) 338, 992, 1190, 1406, 1575, 1689, 1892, 2108, 2276
CviQI GTAC 7 cut(s) 79, 622, 665, 680, 1193, 1236, 2770
DdeI CTNAG 8 cut(s) 239, 393, 641, 1119, 1227, 1658, 2268, 2832
DraI TTTAAA 1 cut(s) 430
DrdI GACNNNNNNGTC 1 cut(s) 869
DseDI GACNNNNNNGTC 1 cut(s) 869
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 3 cut(s) 69, 640, 2311
Eco130I CCWWGG 3 cut(s) 448, 1857, 2524
Eco147I AGGCCT 1 cut(s) 2582
Eco24I GRGCYC 3 cut(s) 71, 642, 2313
Eco31I GGTCTC 1 cut(s) 2330
Eco32I GATATC 1 cut(s) 1804
Eco47I GGWCC 3 cut(s) 263, 1958, 2817
Eco53kI GAGCTC 3 cut(s) 69, 640, 2311
Eco57I CTGAAG 1 cut(s) 1904
EcoICRI GAGCTC 3 cut(s) 69, 640, 2311
EcoO109I RGGNCCY 2 cut(s) 263, 1958
EcoRII CCWGG 2 cut(s) 1960, 2710
EcoRV GATATC 1 cut(s) 1804
EcoT14I CCWWGG 3 cut(s) 448, 1857, 2524
EcoT22I ATGCAT 3 cut(s) 376, 1690, 2659
EcoT38I GRGCYC 3 cut(s) 71, 642, 2313
ErhI CCWWGG 3 cut(s) 448, 1857, 2524
FaeI CATG 9 cut(s) 341, 995, 1193, 1409, 1578, 1692, 1895, 2111, 2279
FalI AAGNNNNNCTT 4 cut(s) 106, 138, 2223, 2255
FaqI GGGAC 4 cut(s) 249, 1754, 1990, 2545
FatI CATG 9 cut(s) 337, 991, 1189, 1405, 1574, 1688, 1891, 2107, 2275
FauI CCCGC 2 cut(s) 421, 772
FauNDI CATATG 1 cut(s) 808
Fnu4HI GCNGC 6 cut(s) 74, 145, 232, 554, 2194, 2795
FokI GGATG 6 cut(s) 752, 936, 1022, 1179, 1285, 2178
FriOI GRGCYC 3 cut(s) 71, 642, 2313
Fsp4HI GCNGC 6 cut(s) 74, 145, 232, 554, 2194, 2795
FspBI CTAG 6 cut(s) 485, 1413, 1853, 2367, 2472, 2750
GlaI GCGC 8 cut(s) 43, 143, 163, 1463, 2197, 2274, 2459, 2748
GluI GCNGC 6 cut(s) 74, 145, 232, 554, 2194, 2795
GsuI CTGGAG 1 cut(s) 2289
HaeII RGCGCY 1 cut(s) 2750
HaeIII GGCC 2 cut(s) 366, 2582
HapII CCGG 5 cut(s) 457, 1339, 1351, 2245, 2788
HgaI GACGC 7 cut(s) 65, 136, 142, 238, 368, 2728, 2800
HhaI GCGC 8 cut(s) 44, 144, 164, 1464, 2198, 2275, 2460, 2749
Hin1I GRCGYC 3 cut(s) 147, 249, 2720
Hin1II CATG 9 cut(s) 341, 995, 1193, 1409, 1578, 1692, 1895, 2111, 2279
Hin6I GCGC 8 cut(s) 42, 142, 162, 1462, 2196, 2273, 2458, 2747
HinP1I GCGC 8 cut(s) 42, 142, 162, 1462, 2196, 2273, 2458, 2747
HincII GTYRAC 2 cut(s) 2665, 2701
HindII GTYRAC 2 cut(s) 2665, 2701
HindIII AAGCTT 3 cut(s) 1133, 1548, 2123
HpaII CCGG 5 cut(s) 457, 1339, 1351, 2245, 2788
HphI GGTGA 7 cut(s) 223, 341, 545, 1916, 1978, 2234, 2507
Hpy99I CGWCG 5 cut(s) 10, 59, 130, 152, 254
HpyCH4IV ACGT 4 cut(s) 570, 1530, 2003, 2667
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 255, 1543
HpyF3I CTNAG 8 cut(s) 239, 393, 641, 1119, 1227, 1658, 2268, 2832
HpySE526I ACGT 4 cut(s) 570, 1530, 2003, 2667
Hsp92I GRCGYC 3 cut(s) 147, 249, 2720
Hsp92II CATG 9 cut(s) 341, 995, 1193, 1409, 1578, 1692, 1895, 2111, 2279
HspAI GCGC 8 cut(s) 42, 142, 162, 1462, 2196, 2273, 2458, 2747
Kpn2I TCCGGA 2 cut(s) 1338, 2787
KspI CCGCGG 1 cut(s) 417
LmnI GCTCC 7 cut(s) 66, 274, 637, 1112, 1276, 1341, 2397
Lsp1109I GCAGC 5 cut(s) 60, 243, 540, 2180, 2781
MaeI CTAG 6 cut(s) 485, 1413, 1853, 2367, 2472, 2750
MaeII ACGT 4 cut(s) 570, 1530, 2003, 2667
MaeIII GTNAC 3 cut(s) 796, 1145, 2495
MbiI CCGCTC 2 cut(s) 779, 2400
MfeI CAATTG 4 cut(s) 915, 1593, 1701, 2283
MflI RGATCY 3 cut(s) 2074, 2388, 2450
MhlI GDGCHC 3 cut(s) 71, 642, 2313
MlyI GAGTC 5 cut(s) 755, 857, 1484, 2461, 2542
MmeI TCCRAC 9 cut(s) 130, 615, 1067, 1294, 1911, 2007, 2161, 2410, 2419
Mph1103I ATGCAT 3 cut(s) 376, 1690, 2659
MroI TCCGGA 2 cut(s) 1338, 2787
MroXI GAANNNNTTC 4 cut(s) 885, 1101, 1569, 1944
MseI TTAA 8 cut(s) 429, 1257, 1539, 1736, 2016, 2148, 2315, 2504
MslI CAYNNNNRTG 1 cut(s) 996
MspA1I CMGCKG 3 cut(s) 416, 553, 1521
MspCI CTTAAG 1 cut(s) 1735
MspI CCGG 5 cut(s) 457, 1339, 1351, 2245, 2788
MspR9I CCNGG 2 cut(s) 1962, 2712
MunI CAATTG 4 cut(s) 915, 1593, 1701, 2283
Mva1269I GAATGC 2 cut(s) 546, 611
MvaI CCWGG 2 cut(s) 1962, 2712
MvnI CGCG 5 cut(s) 42, 136, 144, 162, 416
MwoI GCNNNNNNNGC 3 cut(s) 11, 255, 1543
NdeI CATATG 1 cut(s) 808
NheI GCTAGC 2 cut(s) 1852, 2749
NlaIII CATG 9 cut(s) 341, 995, 1193, 1409, 1578, 1692, 1895, 2111, 2279
NlaIV GGNNCC 3 cut(s) 22, 265, 2760
NmeAIII GCCGAG 4 cut(s) 70, 248, 275, 2779
NmuCI GTSAC 2 cut(s) 1145, 2495
NsiI ATGCAT 3 cut(s) 376, 1690, 2659
NspI RCATGY 2 cut(s) 341, 1692
PaeI GCATGC 1 cut(s) 1692
PceI AGGCCT 1 cut(s) 2582
PciI ACATGT 1 cut(s) 337
PcsI WCGNNNNNNNCGW 1 cut(s) 1818
PctI GAATGC 2 cut(s) 546, 611
PdmI GAANNNNTTC 4 cut(s) 885, 1101, 1569, 1944
PfeI GAWTC 6 cut(s) 701, 944, 1570, 1945, 2248, 2737
PflMI CCANNNNNTGG 1 cut(s) 581
PkrI GCNGC 6 cut(s) 75, 146, 233, 555, 2195, 2796
Ple19I CGATCG 1 cut(s) 12
PleI GAGTC 5 cut(s) 754, 857, 1484, 2461, 2542
PpsI GAGTC 5 cut(s) 754, 857, 1484, 2461, 2542
PpuMI RGGWCCY 2 cut(s) 263, 1958
PscI ACATGT 1 cut(s) 337
PshBI ATTAAT 1 cut(s) 1257
PsiI TTATAA 1 cut(s) 659
Psp124BI GAGCTC 3 cut(s) 71, 642, 2313
Psp1406I AACGTT 2 cut(s) 570, 1530
Psp5II RGGWCCY 2 cut(s) 263, 1958
Psp6I CCWGG 2 cut(s) 1960, 2710
PspGI CCWGG 2 cut(s) 1960, 2710
PspN4I GGNNCC 3 cut(s) 22, 265, 2760
PspPI GGNCC 3 cut(s) 263, 1958, 2817
PspPPI RGGWCCY 2 cut(s) 263, 1958
PstNI CAGNNNCTG 3 cut(s) 556, 1961, 2711
PsuI RGATCY 3 cut(s) 2074, 2388, 2450
PvuI CGATCG 1 cut(s) 12
PvuII CAGCTG 1 cut(s) 553
RsaI GTAC 7 cut(s) 80, 623, 666, 681, 1194, 1237, 2771
RsaNI GTAC 7 cut(s) 79, 622, 665, 680, 1193, 1236, 2770
RseI CAYNNNNRTG 1 cut(s) 996
SacI GAGCTC 3 cut(s) 71, 642, 2313
SacII CCGCGG 1 cut(s) 417
SaqAI TTAA 8 cut(s) 429, 1257, 1539, 1736, 2016, 2148, 2315, 2504
SatI GCNGC 6 cut(s) 74, 145, 232, 554, 2194, 2795
Sau96I GGNCC 3 cut(s) 263, 1958, 2817
SchI GAGTC 5 cut(s) 755, 857, 1484, 2461, 2542
ScrFI CCNGG 2 cut(s) 1962, 2712
SduI GDGCHC 3 cut(s) 71, 642, 2313
SfcI CTRYAG 2 cut(s) 1024, 1872
Sfr303I CCGCGG 1 cut(s) 417
SgrBI CCGCGG 1 cut(s) 417
SinI GGWCC 3 cut(s) 263, 1958, 2817
SmiMI CAYNNNNRTG 1 cut(s) 996
SmlI CTYRAG 2 cut(s) 1735, 2510
SmoI CTYRAG 2 cut(s) 1735, 2510
SphI GCATGC 1 cut(s) 1692
SseBI AGGCCT 1 cut(s) 2582
SspI AATATT 3 cut(s) 885, 1242, 1333
SspMI CTAG 6 cut(s) 485, 1413, 1853, 2367, 2472, 2750
SstI GAGCTC 3 cut(s) 71, 642, 2313
StuI AGGCCT 1 cut(s) 2582
StyD4I CCNGG 2 cut(s) 1960, 2710
StyI CCWWGG 3 cut(s) 448, 1857, 2524
TaiI ACGT 4 cut(s) 573, 1533, 2006, 2670
TaqII GACCGA 2 cut(s) 1728, 2354
TatI WGTACW 3 cut(s) 78, 621, 679
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 6 cut(s) 701, 944, 1570, 1945, 2248, 2737
Tru1I TTAA 8 cut(s) 429, 1257, 1539, 1736, 2016, 2148, 2315, 2504
Tru9I TTAA 8 cut(s) 429, 1257, 1539, 1736, 2016, 2148, 2315, 2504
TscAI CASTG 9 cut(s) 172, 1003, 1437, 1813, 1847, 1893, 2135, 2680, 2712
TseFI GTSAC 2 cut(s) 1145, 2495
TseI GCWGC 5 cut(s) 73, 231, 553, 2193, 2794
Tsp45I GTSAC 2 cut(s) 1145, 2495
TspDTI ATGAA 7 cut(s) 714, 1178, 2076, 2124, 2346, 2388, 2729
TspGWI ACGGA 1 cut(s) 2662
TspRI CASTG 9 cut(s) 172, 1003, 1437, 1813, 1847, 1893, 2135, 2680, 2712
Van91I CCANNNNNTGG 1 cut(s) 581
Vha464I CTTAAG 1 cut(s) 1735
VpaK11BI GGWCC 3 cut(s) 263, 1958, 2817
VspI ATTAAT 1 cut(s) 1257
XapI RAATTY 2 cut(s) 1214, 2519
XbaI TCTAGA 1 cut(s) 2366
XceI RCATGY 2 cut(s) 341, 1692
XcmI CCANNNNNNNNNTGG 1 cut(s) 2531
XmnI GAANNNNTTC 4 cut(s) 885, 1101, 1569, 1944
XspI CTAG 6 cut(s) 485, 1413, 1853, 2367, 2472, 2750
Zsp2I ATGCAT 3 cut(s) 376, 1690, 2659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.