FvH4_6g11240

Monothiol glutaredoxin-S16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015118
Physical Location & Seq
Forward (+)
6693557 .. 6695926
2370 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g11240.t1

Sequence Viewer

Length: 882 bp
ATGGCCACCATCATCCACCTCTCTCCGATTCAAACTCCGCCGCCGTCTCCTCGCTTATTTTCTTCCCAAACTACCCTTCACCTCTCACTCAATTTACACCCCAAGCCCCTCTCCTTTCCCTCCATTTCTCTCCGGCCCTACGCTCCGGCCAAGCCTCGCGCTCTGGTCGTCACTTCGGCTGTCAAGAGCCTATCGGAGACTGAGCTAGTCCCAGTGGACGGTGAGGAAGTCGCCGGAAAATTGCCGTCGGAGACCGGCGTCTACGCCGTTTTTGACCAGAACGGTGAGCTTCAGTTCGTCGGCTTATCGAGAAACATAGCCGCGAGTATTCTTGTTCACCGGAAATCGGTGCCGGAGCTATGCCACTCCGTCAAGGTTGGGATAGTGGATGAACCAGATAGGACGGTTCTGACGGAAGCTTGGAAATCATGGATGGAAGAACACATAAAAGCTACTGGGAAAGTGCCTCCAGGTAATGAATCAGGCAATGCCACCTGGGTGCGGCAGGCACCTAAGAAGAAGAAGCCTGATCTGCGGCTGACACCAGGTCGTCACATGCAGTTGACAGTTCCGTTGGAGCAACTTGTGGATCGGTTGGTGAAGGAGAACAAGGTTGTGGCATTTATCAAGGGTTCAAGAAGTGCCCCGTTGTGTGGTTTCTCGCAGAGAGTTATTGGCATTCTTGAAAACCAAGGGGTAGATTATGAGAGTGTTGATGTGCTTGATGAAGAGTATAACTCTGGATTGAGGGAGACTTTGAAGAGCTATAGCAATTGGCCTACTTTCCCGCAAATATTTGTAAACGGAGAACTGGTTGGGGGATGTGATATTCTAACATCCATGCACGAAAAGGGCGAGCTTGCTAGCTTGTTTAAGAAGTGA

Protein Analysis

294

Amino Acids

32.23

Weight (kDa)

7.75

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 205 - 272 8.8e-18 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38270
fragaria_vesca FvH4_6g11240
malus_domestica MD00G1172400.v1.1
prunus_persica Prupe.6G279600_v2.0.a1
pyrus_communis pycom12g16270
rosa_chinensis RchiOBHm_Chr3g0462751
rosa_laevigata RLG00000024835
rosa_multiflora Rmu_co8134540.1_g000001 Rmu_sc0000766.1_g000004
rosa_roxburghii Rroxscaffold_6G00417230
rosa_rugosa Rorug03G0059000
rosa_samantha Rh3AG117000 Rh3BG120700 Rh3CG123700 Rh3DG122400
rosa_wichuraiana Rw3G009910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 349, 508
AccI GTMKAC 1 cut(s) 261
AccII CGCG 2 cut(s) 159, 323
AciI CCGC 6 cut(s) 38, 41, 321, 502, 535, 788
AclWI GGATC 1 cut(s) 597
AcoI YGGCCR 2 cut(s) 3, 147
AcuI CTGAAG 1 cut(s) 275
AcyI GRCGYC 1 cut(s) 258
AfiI CCNNNNNNNGG 4 cut(s) 218, 346, 501, 653
AgsI TTSAA 4 cut(s) 32, 636, 686, 760
AhdI GACNNNNNGTC 1 cut(s) 546
AjnI CCWGG 3 cut(s) 469, 494, 544
AjuI GAANNNNNNNTTGG 2 cut(s) 60, 92
AleI CACNNNNGTG 1 cut(s) 497
AluBI AGCT 8 cut(s) 205, 289, 358, 419, 452, 765, 859, 867
AluI AGCT 8 cut(s) 205, 289, 358, 419, 452, 765, 859, 867
Alw26I GTCTC 4 cut(s) 51, 191, 245, 746
AlwI GGATC 1 cut(s) 597
AoxI GGCC 4 cut(s) 3, 134, 147, 776
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 135
AsuHPI GGTGA 5 cut(s) 71, 233, 296, 329, 610
AsuNHI GCTAGC 1 cut(s) 863
BaeGI GKGCMC 1 cut(s) 646
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 349, 508
BccI CCATC 2 cut(s) 17, 427
BceAI ACGGC 3 cut(s) 28, 229, 251
BciT130I CCWGG 3 cut(s) 471, 496, 546
BcoDI GTCTC 4 cut(s) 51, 191, 245, 746
BfaI CTAG 2 cut(s) 206, 864
BfmI CTRYAG 1 cut(s) 766
BisI GCNGC 4 cut(s) 41, 321, 503, 536
BlsI GCNGC 4 cut(s) 42, 322, 504, 537
Bme1390I CCNGG 3 cut(s) 471, 496, 546
BmeRI GACNNNNNGTC 1 cut(s) 546
BmgT120I GGNCC 1 cut(s) 135
BmiI GGNNCC 2 cut(s) 351, 510
BmrFI CCNGG 3 cut(s) 471, 496, 546
BmrI ACTGGG 2 cut(s) 206, 465
BmtI GCTAGC 1 cut(s) 867
BmuI ACTGGG 2 cut(s) 206, 465
BoxI GACNNNNGTC 1 cut(s) 257
BplI GAGNNNNNCTC 2 cut(s) 722, 754
BpmI CTGGAG 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 495, 691
BsaWI WCCGGW 1 cut(s) 339
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 346, 501, 653
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 3 cut(s) 212, 460, 816
Bse3DI GCAATG 1 cut(s) 493
BseBI CCWGG 3 cut(s) 471, 496, 546
BseDI CCNNGG 2 cut(s) 495, 691
BseGI GGATG 5 cut(s) 12, 394, 438, 827, 836
BseLI CCNNNNNNNGG 4 cut(s) 218, 346, 501, 653
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 3 cut(s) 212, 460, 816
BseRI GAGGAG 1 cut(s) 39
BseSI GKGCMC 1 cut(s) 646
Bsh1236I CGCG 2 cut(s) 159, 323
BshFI GGCC 4 cut(s) 5, 136, 149, 778
BshNI GGYRCC 2 cut(s) 349, 508
BsiSI CCGG 6 cut(s) 133, 146, 234, 255, 340, 353
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 4 cut(s) 218, 346, 501, 653
BsmAI GTCTC 4 cut(s) 51, 191, 245, 746
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 4 cut(s) 5, 136, 149, 778
Bso31I GGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 2 cut(s) 529, 589
BspACI CCGC 6 cut(s) 38, 41, 321, 502, 535, 788
BspANI GGCC 4 cut(s) 5, 136, 149, 778
BspCNI CTCAG 1 cut(s) 193
BspFNI CGCG 2 cut(s) 159, 323
BspLI GGNNCC 2 cut(s) 351, 510
BspOI GCTAGC 1 cut(s) 867
BspPI GGATC 1 cut(s) 597
BspQI GCTCTTC 1 cut(s) 755
BspT107I GGYRCC 2 cut(s) 349, 508
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 3 cut(s) 212, 460, 816
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 495, 691
BssMI GATC 2 cut(s) 529, 589
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 1 cut(s) 691
Bst2UI CCWGG 3 cut(s) 471, 496, 546
Bst4CI ACNGT 4 cut(s) 221, 284, 406, 568
Bst6I CTCTTC 2 cut(s) 723, 755
BstACI GRCGYC 1 cut(s) 258
BstC8I GCNNGC 4 cut(s) 507, 857, 861, 865
BstDEI CTNAG 2 cut(s) 201, 513
BstF5I GGATG 5 cut(s) 12, 394, 438, 827, 836
BstFNI CGCG 2 cut(s) 159, 323
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 2 cut(s) 532, 592
BstMAI GTCTC 4 cut(s) 51, 191, 245, 746
BstMBI GATC 2 cut(s) 529, 589
BstMWI GCNNNNNNNGC 1 cut(s) 532
BstNI CCWGG 3 cut(s) 471, 496, 546
BstNSI RCATGY 1 cut(s) 559
BstPAI GACNNNNGTC 1 cut(s) 257
BstSCI CCNGG 3 cut(s) 469, 494, 544
BstSFI CTRYAG 1 cut(s) 766
BstSLI GKGCMC 1 cut(s) 646
BstUI CGCG 2 cut(s) 159, 323
BsuRI GGCC 4 cut(s) 5, 136, 149, 778
BtsCI GGATG 5 cut(s) 12, 394, 438, 827, 836
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 4 cut(s) 507, 857, 861, 865
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 1 cut(s) 135
CseI GACGC 1 cut(s) 247
CsiI ACCWGGT 1 cut(s) 544
CviAII CATG 3 cut(s) 429, 556, 841
DdeI CTNAG 2 cut(s) 201, 513
DpnI GATC 2 cut(s) 531, 591
DpnII GATC 2 cut(s) 529, 589
DriI GACNNNNNGTC 1 cut(s) 546
EaeI YGGCCR 2 cut(s) 3, 147
Eam1104I CTCTTC 2 cut(s) 723, 755
Eam1105I GACNNNNNGTC 1 cut(s) 546
EarI CTCTTC 2 cut(s) 723, 755
EciI GGCGGA 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 691
Eco31I GGTCTC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 275
EcoRII CCWGG 3 cut(s) 469, 494, 544
EcoT14I CCWWGG 1 cut(s) 691
ErhI CCWWGG 1 cut(s) 691
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 3 cut(s) 432, 559, 844
FaiI YATR 9 cut(s) 317, 361, 430, 446, 557, 705, 735, 768, 842
FaqI GGGAC 1 cut(s) 194
FatI CATG 3 cut(s) 428, 555, 840
FauI CCCGC 1 cut(s) 795
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 4 cut(s) 41, 321, 503, 536
FokI GGATG 4 cut(s) 401, 445, 823, 834
Fsp4HI GCNGC 4 cut(s) 41, 321, 503, 536
FspBI CTAG 2 cut(s) 206, 864
GlaI GCGC 1 cut(s) 160
GluI GCNGC 4 cut(s) 41, 321, 503, 536
GsuI CTGGAG 1 cut(s) 453
HaeIII GGCC 4 cut(s) 5, 136, 149, 778
HapII CCGG 6 cut(s) 133, 146, 234, 255, 340, 353
HgaI GACGC 1 cut(s) 247
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 3 cut(s) 432, 559, 844
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 564
HindII GTYRAC 1 cut(s) 564
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 2 cut(s) 28, 479
HpaII CCGG 6 cut(s) 133, 146, 234, 255, 340, 353
HphI GGTGA 5 cut(s) 71, 233, 296, 329, 610
Hpy166II GTNNAC 5 cut(s) 217, 262, 337, 564, 802
Hpy188I TCNGA 4 cut(s) 27, 196, 250, 411
Hpy188III TCNNGA 5 cut(s) 184, 309, 636, 683, 741
Hpy8I GTNNAC 5 cut(s) 217, 262, 337, 564, 802
Hpy99I CGWCG 2 cut(s) 250, 302
HpyAV CCTTC 2 cut(s) 86, 595
HpyCH4III ACNGT 4 cut(s) 221, 284, 406, 568
HpyCH4V TGCA 2 cut(s) 559, 844
HpyF10VI GCNNNNNNNGC 1 cut(s) 532
HpyF3I CTNAG 2 cut(s) 201, 513
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 3 cut(s) 432, 559, 844
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 2 cut(s) 529, 589
LguI GCTCTTC 1 cut(s) 755
LmnI GCTCC 3 cut(s) 148, 355, 577
MabI ACCWGGT 1 cut(s) 544
MaeI CTAG 2 cut(s) 206, 864
MaeIII GTNAC 2 cut(s) 169, 551
MalI GATC 2 cut(s) 531, 591
MboI GATC 2 cut(s) 529, 589
MboII GAAGA 6 cut(s) 54, 449, 529, 532, 740, 772
MfeI CAATTG 1 cut(s) 772
MhlI GDGCHC 1 cut(s) 646
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 91, 239, 772
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 228, 555
MnlI CCTC 9 cut(s) 29, 60, 92, 119, 130, 165, 217, 477, 741
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 873
MslI CAYNNNNRTG 1 cut(s) 497
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 6 cut(s) 133, 146, 234, 255, 340, 353
MspR9I CCNGG 3 cut(s) 471, 496, 546
MunI CAATTG 1 cut(s) 772
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 3 cut(s) 471, 496, 546
MvnI CGCG 2 cut(s) 159, 323
MwoI GCNNNNNNNGC 1 cut(s) 532
NdeII GATC 2 cut(s) 529, 589
NheI GCTAGC 1 cut(s) 863
NlaIII CATG 3 cut(s) 432, 559, 844
NlaIV GGNNCC 2 cut(s) 351, 510
NmuCI GTSAC 2 cut(s) 169, 551
NspI RCATGY 1 cut(s) 559
OliI CACNNNNGTG 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 755
PcsI WCGNNNNNNNCGW 1 cut(s) 852
PctI GAATGC 1 cut(s) 678
PfeI GAWTC 2 cut(s) 28, 479
PkrI GCNGC 4 cut(s) 42, 322, 504, 537
PshAI GACNNNNGTC 1 cut(s) 257
Psp6I CCWGG 3 cut(s) 469, 494, 544
PspGI CCWGG 3 cut(s) 469, 494, 544
PspN4I GGNNCC 2 cut(s) 351, 510
PspPI GGNCC 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 755
SaqAI TTAA 1 cut(s) 873
SatI GCNGC 4 cut(s) 41, 321, 503, 536
Sau3AI GATC 2 cut(s) 529, 589
Sau96I GGNCC 1 cut(s) 135
ScrFI CCNGG 3 cut(s) 471, 496, 546
SduI GDGCHC 1 cut(s) 646
SexAI ACCWGGT 1 cut(s) 544
SfcI CTRYAG 1 cut(s) 766
SmiMI CAYNNNNRTG 1 cut(s) 497
Sse9I AATT 3 cut(s) 91, 239, 772
SsiI CCGC 6 cut(s) 38, 41, 321, 502, 535, 788
SspI AATATT 1 cut(s) 795
SspMI CTAG 2 cut(s) 206, 864
StyD4I CCNGG 3 cut(s) 469, 494, 544
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 4 cut(s) 221, 284, 406, 568
TaqI TCGA 1 cut(s) 308
TasI AATT 3 cut(s) 91, 239, 772
TauI GCSGC 4 cut(s) 43, 323, 505, 538
TfiI GAWTC 2 cut(s) 28, 479
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 2 cut(s) 169, 551
Tsp45I GTSAC 2 cut(s) 169, 551
TspDTI ATGAA 3 cut(s) 405, 492, 741
TspGWI ACGGA 4 cut(s) 358, 428, 561, 819
TspRI CASTG 1 cut(s) 219
XceI RCATGY 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 2 cut(s) 206, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.