RLG00000024835

Monothiol glutaredoxin-S16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015118
Physical Location & Seq
Reverse (-)
39506587 .. 39508393
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024835

Sequence Viewer

Length: 891 bp
ATGGCCACAATCATCCACCTCTCTCCGATTCACACTTCGCCGCCGTCTCCTCGCTTATTTTCTCCCCAATCTTCCCAAAATACCCTTCACCTCTCATTCAATTCACACCCCAAACCCCTCTCCCTTCCCTCCATTTCTCTCAAGCCCTGCGCTCCGGCCAGGCCTCGCGCTTTGGTCGTCACTTCGGCTGTTAAGAGCCTCTCGGAGACTGAGCTAGTCTCAGTGGATGGCGAAGAAGTCGCCGGAAAATTGCCGTCGGAGACCGGCGTCTACGCCGTTTTTGACCAGAACGGTGAGCTTCAGTTCGTCGGCTTATCGAGAAACATAGCGGCGAGTGTTCTTGTTCACCGGAAATCGGTGCCGGAGCTGTGCCACTCCGTCAAGGTTGGGATAGTAGATGAACCAGATAGGACGGTTCTAACTGAAGCTTGGAAATCATGGATGGAAGAACACATAAAAGCTACAGGGAAAGTGCCTCCAGGAAATGAATCAGGCAACGCCACCTGGGTCCGGCCAGCACCTAAGAAGAAGAAGCCTGATTTGCGGCTGACACCAGGTCGTCACGTGCAGTTAACAGTTCCATTGGAGCAACTCGTGGATCGGTTGGTGAAGGAGAACAAGGTTGTGGCCTTTATCAAGGGTTCGAGAAGCGCCCCATTGTGTGGTTTCTCACAGAGGGTTGTTGGCATTCTTGAAAATCAAGGGGTGGATTATGAGAGCGTTGATGTGCTTGACGAAGAGTATAACTCCGGATTGAGGGAGACTCTCAAGACCTATAGTAATTGGCCTACTTTCCCGCAGATATTTGTGAATGGAGAACTGGTCGGGGGTTGCGATATTCTAACATCCATGCACGAAAAGGGAGAGCTTGCTAGCTTGCTTAAGAAATGA

Protein Analysis

297

Amino Acids

32.35

Weight (kDa)

7.73

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 208 - 275 9.5e-18 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38270
fragaria_vesca FvH4_6g11240
malus_domestica MD00G1172400.v1.1
prunus_persica Prupe.6G279600_v2.0.a1
pyrus_communis pycom12g16270
rosa_chinensis RchiOBHm_Chr3g0462751
rosa_laevigata RLG00000024835
rosa_multiflora Rmu_co8134540.1_g000001 Rmu_sc0000766.1_g000004
rosa_roxburghii Rroxscaffold_6G00417230
rosa_rugosa Rorug03G0059000
rosa_samantha Rh3AG117000 Rh3BG120700 Rh3CG123700 Rh3DG122400
rosa_wichuraiana Rw3G009910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 662
AccI GTMKAC 1 cut(s) 270
AccII CGCG 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 749
AciI CCGC 4 cut(s) 41, 329, 544, 797
AclWI GGATC 1 cut(s) 606
AcoI YGGCCR 3 cut(s) 3, 156, 512
AcuI CTGAAG 2 cut(s) 284, 444
AcvI CACGTG 1 cut(s) 565
AcyI GRCGYC 1 cut(s) 267
AfiI CCNNNNNNNGG 4 cut(s) 355, 510, 662, 756
AflII CTTAAG 1 cut(s) 881
AgsI TTSAA 2 cut(s) 100, 695
AhdI GACNNNNNGTC 1 cut(s) 555
AjnI CCWGG 4 cut(s) 158, 478, 503, 553
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AluBI AGCT 7 cut(s) 214, 298, 367, 428, 461, 868, 876
AluI AGCT 7 cut(s) 214, 298, 367, 428, 461, 868, 876
Alw26I GTCTC 5 cut(s) 51, 200, 223, 254, 755
AlwI GGATC 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 749
AoxI GGCC 6 cut(s) 3, 156, 161, 512, 627, 785
AspLEI GCGC 3 cut(s) 152, 170, 653
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 4 cut(s) 80, 305, 338, 619
AsuNHI GCTAGC 1 cut(s) 872
AvaII GGWCC 1 cut(s) 508
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 358
BauI CACGAG 1 cut(s) 593
BbrPI CACGTG 1 cut(s) 565
BccI CCATC 2 cut(s) 221, 436
BceAI ACGGC 3 cut(s) 28, 238, 260
BciT130I CCWGG 4 cut(s) 160, 480, 505, 555
BcoDI GTCTC 5 cut(s) 51, 200, 223, 254, 755
BfaI CTAG 2 cut(s) 215, 873
BfmI CTRYAG 2 cut(s) 462, 775
BfoI RGCGCY 1 cut(s) 654
BfrI CTTAAG 1 cut(s) 881
BisI GCNGC 3 cut(s) 41, 330, 545
BlsI GCNGC 3 cut(s) 42, 331, 546
Bme1390I CCNGG 4 cut(s) 160, 480, 505, 555
Bme18I GGWCC 1 cut(s) 508
BmeRI GACNNNNNGTC 1 cut(s) 555
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 2 cut(s) 360, 509
BmrFI CCNGG 4 cut(s) 160, 480, 505, 555
BmtI GCTAGC 1 cut(s) 876
BoxI GACNNNNGTC 1 cut(s) 266
BplI GAGNNNNNCTC 6 cut(s) 203, 235, 731, 763, 748, 780
BpmI CTGGAG 1 cut(s) 462
BpuEI CTTGAG 2 cut(s) 125, 752
BsaAI YACGTR 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 504
BsaWI WCCGGW 2 cut(s) 348, 749
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 510, 662, 756
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 825
BseAI TCCGGA 1 cut(s) 749
BseBI CCWGG 4 cut(s) 160, 480, 505, 555
BseDI CCNNGG 1 cut(s) 504
BseGI GGATG 4 cut(s) 12, 232, 447, 845
BseLI CCNNNNNNNGG 4 cut(s) 355, 510, 662, 756
BseMII CTCAG 2 cut(s) 201, 234
BseNI ACTGG 1 cut(s) 825
BseRI GAGGAG 1 cut(s) 39
BsgI GTGCAG 1 cut(s) 587
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
BshNI GGYRCC 1 cut(s) 358
BsiSI CCGG 7 cut(s) 155, 243, 264, 349, 362, 511, 750
BslI CCNNNNNNNGG 4 cut(s) 355, 510, 662, 756
BsmAI GTCTC 5 cut(s) 51, 200, 223, 254, 755
BsmBI CGTCTC 1 cut(s) 51
BsmI GAATGC 1 cut(s) 687
BsnI GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
Bso31I GGTCTC 1 cut(s) 254
Bsp13I TCCGGA 1 cut(s) 749
Bsp143I GATC 1 cut(s) 598
BspACI CCGC 4 cut(s) 41, 329, 544, 797
BspANI GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
BspCNI CTCAG 2 cut(s) 202, 233
BspEI TCCGGA 1 cut(s) 749
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 360, 509
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 1 cut(s) 606
BspT107I GGYRCC 1 cut(s) 358
BspTI CTTAAG 1 cut(s) 881
BspTNI GGTCTC 1 cut(s) 254
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 1 cut(s) 825
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 593
Bst2BI CACGAG 1 cut(s) 593
Bst2UI CCWGG 4 cut(s) 160, 480, 505, 555
Bst4CI ACNGT 3 cut(s) 293, 415, 577
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 1 cut(s) 267
BstAFI CTTAAG 1 cut(s) 881
BstBAI YACGTR 1 cut(s) 565
BstC8I GCNNGC 4 cut(s) 516, 870, 874, 878
BstDEI CTNAG 3 cut(s) 210, 220, 522
BstF5I GGATG 4 cut(s) 12, 232, 447, 845
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 654
BstHHI GCGC 3 cut(s) 152, 170, 653
BstKTI GATC 1 cut(s) 601
BstMAI GTCTC 5 cut(s) 51, 200, 223, 254, 755
BstMBI GATC 1 cut(s) 598
BstMWI GCNNNNNNNGC 1 cut(s) 541
BstNI CCWGG 4 cut(s) 160, 480, 505, 555
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 4 cut(s) 158, 478, 503, 553
BstSFI CTRYAG 2 cut(s) 462, 775
BstUI CGCG 1 cut(s) 168
BsuRI GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
BtsCI GGATG 4 cut(s) 12, 232, 447, 845
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 4 cut(s) 516, 870, 874, 878
CfoI GCGC 3 cut(s) 152, 170, 653
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 508
CseI GACGC 1 cut(s) 256
CsiI ACCWGGT 1 cut(s) 553
CviAII CATG 2 cut(s) 438, 850
DdeI CTNAG 3 cut(s) 210, 220, 522
DpnI GATC 1 cut(s) 600
DpnII GATC 1 cut(s) 598
DriI GACNNNNNGTC 1 cut(s) 555
EaeI YGGCCR 3 cut(s) 3, 156, 512
Eam1104I CTCTTC 1 cut(s) 732
Eam1105I GACNNNNNGTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 732
Eco147I AGGCCT 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 508
Eco57I CTGAAG 2 cut(s) 284, 444
Eco72I CACGTG 1 cut(s) 565
EcoRII CCWGG 4 cut(s) 158, 478, 503, 553
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 2 cut(s) 441, 853
FaiI YATR 7 cut(s) 326, 439, 455, 714, 744, 777, 851
FatI CATG 2 cut(s) 437, 849
FauI CCCGC 1 cut(s) 804
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 3 cut(s) 41, 330, 545
FokI GGATG 3 cut(s) 239, 454, 832
Fsp4HI GCNGC 3 cut(s) 41, 330, 545
FspBI CTAG 2 cut(s) 215, 873
GlaI GCGC 3 cut(s) 151, 169, 652
GluI GCNGC 3 cut(s) 41, 330, 545
GsuI CTGGAG 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 654
HaeIII GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
HapII CCGG 7 cut(s) 155, 243, 264, 349, 362, 511, 750
HgaI GACGC 1 cut(s) 256
HhaI GCGC 3 cut(s) 152, 170, 653
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 2 cut(s) 441, 853
Hin6I GCGC 3 cut(s) 150, 168, 651
HinP1I GCGC 3 cut(s) 150, 168, 651
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 3 cut(s) 28, 488, 763
HpaI GTTAAC 1 cut(s) 573
HpaII CCGG 7 cut(s) 155, 243, 264, 349, 362, 511, 750
HphI GGTGA 4 cut(s) 80, 305, 338, 619
Hpy166II GTNNAC 3 cut(s) 271, 346, 573
Hpy188I TCNGA 3 cut(s) 27, 205, 259
Hpy188III TCNNGA 5 cut(s) 318, 645, 692, 750, 769
Hpy8I GTNNAC 3 cut(s) 271, 346, 573
Hpy99I CGWCG 2 cut(s) 259, 311
HpyAV CCTTC 3 cut(s) 95, 134, 604
HpyCH4III ACNGT 3 cut(s) 293, 415, 577
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 2 cut(s) 568, 853
HpyF10VI GCNNNNNNNGC 1 cut(s) 541
HpyF3I CTNAG 3 cut(s) 210, 220, 522
HpySE526I ACGT 1 cut(s) 564
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 2 cut(s) 441, 853
HspAI GCGC 3 cut(s) 150, 168, 651
Kpn2I TCCGGA 1 cut(s) 749
KspAI GTTAAC 1 cut(s) 573
Kzo9I GATC 1 cut(s) 598
LmnI GCTCC 3 cut(s) 157, 364, 586
MabI ACCWGGT 1 cut(s) 553
MaeI CTAG 2 cut(s) 215, 873
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 2 cut(s) 178, 560
MalI GATC 1 cut(s) 600
MboI GATC 1 cut(s) 598
MboII GAAGA 6 cut(s) 63, 245, 458, 538, 541, 749
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 100, 248, 781
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 757
MmeI TCCRAC 1 cut(s) 237
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 749
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 192, 572, 882
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 881
MspI CCGG 7 cut(s) 155, 243, 264, 349, 362, 511, 750
MspR9I CCNGG 4 cut(s) 160, 480, 505, 555
Mva1269I GAATGC 1 cut(s) 687
MvaI CCWGG 4 cut(s) 160, 480, 505, 555
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 541
NdeII GATC 1 cut(s) 598
NheI GCTAGC 1 cut(s) 872
NlaIII CATG 2 cut(s) 441, 853
NlaIV GGNNCC 2 cut(s) 360, 509
NmuCI GTSAC 2 cut(s) 178, 560
PceI AGGCCT 1 cut(s) 163
PcsI WCGNNNNNNNCGW 1 cut(s) 831
PctI GAATGC 1 cut(s) 687
PfeI GAWTC 2 cut(s) 28, 488
PflMI CCANNNNNTGG 1 cut(s) 662
PfoI TCCNGGA 1 cut(s) 478
PkrI GCNGC 3 cut(s) 42, 331, 546
PleI GAGTC 1 cut(s) 757
PmaCI CACGTG 1 cut(s) 565
PmlI CACGTG 1 cut(s) 565
PpsI GAGTC 1 cut(s) 757
Ppu21I YACGTR 1 cut(s) 565
PshAI GACNNNNGTC 1 cut(s) 266
Psp6I CCWGG 4 cut(s) 158, 478, 503, 553
PspCI CACGTG 1 cut(s) 565
PspGI CCWGG 4 cut(s) 158, 478, 503, 553
PspN4I GGNNCC 2 cut(s) 360, 509
PspPI GGNCC 1 cut(s) 508
SaqAI TTAA 3 cut(s) 192, 572, 882
SatI GCNGC 3 cut(s) 41, 330, 545
Sau3AI GATC 1 cut(s) 598
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 1 cut(s) 757
ScrFI CCNGG 4 cut(s) 160, 480, 505, 555
SexAI ACCWGGT 1 cut(s) 553
SfcI CTRYAG 2 cut(s) 462, 775
SinI GGWCC 1 cut(s) 508
SmlI CTYRAG 3 cut(s) 140, 767, 881
SmoI CTYRAG 3 cut(s) 140, 767, 881
Sse9I AATT 3 cut(s) 100, 248, 781
SseBI AGGCCT 1 cut(s) 163
SsiI CCGC 4 cut(s) 41, 329, 544, 797
SspMI CTAG 2 cut(s) 215, 873
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 4 cut(s) 158, 478, 503, 553
TaaI ACNGT 3 cut(s) 293, 415, 577
TaiI ACGT 1 cut(s) 567
TaqI TCGA 2 cut(s) 317, 644
TasI AATT 3 cut(s) 100, 248, 781
TauI GCSGC 3 cut(s) 43, 332, 547
TfiI GAWTC 2 cut(s) 28, 488
Tru1I TTAA 3 cut(s) 192, 572, 882
Tru9I TTAA 3 cut(s) 192, 572, 882
TscAI CASTG 1 cut(s) 228
TseFI GTSAC 2 cut(s) 178, 560
Tsp45I GTSAC 2 cut(s) 178, 560
TspDTI ATGAA 2 cut(s) 414, 501
TspGWI ACGGA 1 cut(s) 367
TspRI CASTG 1 cut(s) 228
Van91I CCANNNNNTGG 1 cut(s) 662
Vha464I CTTAAG 1 cut(s) 881
VpaK11BI GGWCC 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 2 cut(s) 215, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.