MD00G1172400.v1.1

Monothiol glutaredoxin-S16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015118
Physical Location & Seq
Reverse (-)
39568138 .. 39570272
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1172400.v1.1.491

Sequence Viewer

Length: 363 bp
ATGGAAGAACACATAAAAGCAACCGGGAAGGTTCCTCCGGGAAATGAATCAGGCAATGCCATATGGGTCCGAAAGCCACCACCAAGGAAGAAGAAGCCTGTCGTGGCCTTTATTAAGGGCTCGAGAAGTGCCCCATTGTGCGGATTTTCGCAGAGGGTTGTTGGCATTATTGAAAACCAGGGGGTGGATTATGAGAGCGTTGATGTGCTTGATGAAGAGTATAATAGTGGATTGAGGGAGACTCTGAAGAAGTATAGTAACTGGCCAACTTTCCCGCAGATATTTGTGAATGGGCTTGGGGGCTGTGATATTCTAGGTTCCATGCAGGAGAAGGGTGAGCTTTCCAGCTTGTTTAAAAAGTGA

Protein Analysis

121

Amino Acids

13.25

Weight (kDa)

8.91

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 34 - 98 8.4e-17 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38270
fragaria_vesca FvH4_6g11240
malus_domestica MD00G1172400.v1.1
prunus_persica Prupe.6G279600_v2.0.a1
pyrus_communis pycom12g16270
rosa_chinensis RchiOBHm_Chr3g0462751
rosa_laevigata RLG00000024835
rosa_multiflora Rmu_co8134540.1_g000001 Rmu_sc0000766.1_g000004
rosa_roxburghii Rroxscaffold_6G00417230
rosa_rugosa Rorug03G0059000
rosa_samantha Rh3AG117000 Rh3BG120700 Rh3CG123700 Rh3DG122400
rosa_wichuraiana Rw3G009910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 184
AciI CCGC 2 cut(s) 141, 275
AcoI YGGCCR 1 cut(s) 263
AcuI CTGAAG 1 cut(s) 266
AfiI CCNNNNNNNGG 2 cut(s) 140, 184
AgsI TTSAA 1 cut(s) 173
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 2 cut(s) 340, 348
AluI AGCT 2 cut(s) 340, 348
Alw26I GTCTC 1 cut(s) 233
Ama87I CYCGRG 1 cut(s) 121
AoxI GGCC 2 cut(s) 105, 263
AspS9I GGNCC 1 cut(s) 67
AsuC2I CCSGG 2 cut(s) 25, 39
AsuHPI GGTGA 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 121
AvaII GGWCC 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 133
BalI TGGCCA 1 cut(s) 265
BanII GRGCYC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 179
BcnI CCSGG 2 cut(s) 25, 39
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 1 cut(s) 314
Bme1390I CCNGG 3 cut(s) 25, 39, 179
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 3 cut(s) 33, 68, 319
BmrFI CCNGG 3 cut(s) 25, 39, 179
BplI GAGNNNNNCTC 2 cut(s) 226, 258
BpuMI CCSGG 2 cut(s) 25, 39
BsaJI CCNNGG 2 cut(s) 83, 178
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 184
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 61
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 2 cut(s) 83, 178
BseLI CCNNNNNNNGG 2 cut(s) 140, 184
BseMI GCAATG 1 cut(s) 61
BseNI ACTGG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 133
BshFI GGCC 2 cut(s) 107, 265
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 2 cut(s) 24, 38
BslI CCNNNNNNNGG 2 cut(s) 140, 184
BsmAI GTCTC 1 cut(s) 233
BsnI GGCC 2 cut(s) 107, 265
BsoBI CYCGRG 1 cut(s) 121
Bsp1286I GDGCHC 2 cut(s) 122, 133
BspACI CCGC 2 cut(s) 141, 275
BspANI GGCC 2 cut(s) 107, 265
BspLI GGNNCC 3 cut(s) 33, 68, 319
BsrDI GCAATG 1 cut(s) 61
BsrI ACTGG 1 cut(s) 266
BssECI CCNNGG 2 cut(s) 83, 178
BssT1I CCWWGG 1 cut(s) 83
Bst2UI CCWGG 1 cut(s) 179
Bst6I CTCTTC 1 cut(s) 210
BstMAI GTCTC 1 cut(s) 233
BstNI CCWGG 1 cut(s) 179
BstSCI CCNGG 3 cut(s) 23, 37, 177
BstSLI GKGCMC 1 cut(s) 133
BsuRI GGCC 2 cut(s) 107, 265
Cfr13I GGNCC 1 cut(s) 67
CviAII CATG 1 cut(s) 322
CviJI RGCY 9 cut(s) 76, 97, 107, 120, 265, 295, 303, 340, 348
CviKI_1 RGCY 9 cut(s) 76, 97, 107, 120, 265, 295, 303, 340, 348
DraI TTTAAA 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 263
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 83
EcoT38I GRGCYC 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 83
FaeI CATG 1 cut(s) 325
FaiI YATR 7 cut(s) 14, 62, 64, 192, 222, 255, 323
FatI CATG 1 cut(s) 321
FauI CCCGC 1 cut(s) 282
FauNDI CATATG 1 cut(s) 62
FriOI GRGCYC 1 cut(s) 122
FspBI CTAG 1 cut(s) 314
HaeIII GGCC 2 cut(s) 107, 265
HapII CCGG 2 cut(s) 24, 38
Hin1II CATG 1 cut(s) 325
HinfI GANTC 2 cut(s) 47, 241
HpaII CCGG 2 cut(s) 24, 38
HphI GGTGA 1 cut(s) 347
Hpy188I TCNGA 2 cut(s) 71, 246
Hpy188III TCNNGA 1 cut(s) 123
HpyAV CCTTC 2 cut(s) 22, 325
HpyCH4V TGCA 1 cut(s) 325
Hsp92II CATG 1 cut(s) 325
LpnPI CCDG 9 cut(s) 36, 37, 51, 111, 164, 191, 247, 311, 358
MaeI CTAG 1 cut(s) 314
MaeIII GTNAC 1 cut(s) 257
MboII GAAGA 5 cut(s) 17, 100, 103, 227, 259
MhlI GDGCHC 2 cut(s) 122, 133
MlsI TGGCCA 1 cut(s) 265
MluNI TGGCCA 1 cut(s) 265
MlyI GAGTC 1 cut(s) 235
MnlI CCTC 3 cut(s) 45, 147, 228
Mox20I TGGCCA 1 cut(s) 265
MscI TGGCCA 1 cut(s) 265
MseI TTAA 2 cut(s) 114, 354
Msp20I TGGCCA 1 cut(s) 265
MspI CCGG 2 cut(s) 24, 38
MspR9I CCNGG 3 cut(s) 25, 39, 179
MvaI CCWGG 1 cut(s) 179
NciI CCSGG 2 cut(s) 25, 39
NdeI CATATG 1 cut(s) 62
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 3 cut(s) 33, 68, 319
PaeR7I CTCGAG 1 cut(s) 121
PfeI GAWTC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 184
PfoI TCCNGGA 1 cut(s) 37
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 3 cut(s) 33, 68, 319
PspPI GGNCC 1 cut(s) 67
SaqAI TTAA 2 cut(s) 114, 354
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 3 cut(s) 25, 39, 179
SduI GDGCHC 2 cut(s) 122, 133
SetI ASST 4 cut(s) 33, 319, 342, 350
Sfr274I CTCGAG 1 cut(s) 121
SinI GGWCC 1 cut(s) 67
SlaI CTCGAG 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
SsiI CCGC 2 cut(s) 141, 275
SspMI CTAG 1 cut(s) 314
StyD4I CCNGG 3 cut(s) 23, 37, 177
StyI CCWWGG 1 cut(s) 83
TaqI TCGA 1 cut(s) 122
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 2 cut(s) 114, 354
Tru9I TTAA 2 cut(s) 114, 354
TspDTI ATGAA 2 cut(s) 60, 228
Van91I CCANNNNNTGG 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 67
XhoI CTCGAG 1 cut(s) 121
XspI CTAG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.