pycom12g16270

Monothiol glutaredoxin-S16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015118
Physical Location & Seq
Reverse (-)
18657392 .. 18658836
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g16270.1

Sequence Viewer

Length: 897 bp
ATGGCGACAATCATCCACCTCTCTCCAATCCACACTTCATCTCCCTCTAGCCGCTTATTCTCTTCTCACTCTCCCCAAAATACCCCCAACCCCTCATTCAATTTACCAACCAAAGCCCCTACCTTCCGCTCCATTTCTCTCAAACCTTACGCCGCATCCAAGCCCCGAGCTTCGGTCATCACTTCGGCAGTTAAGGCCCTCTCGGAGACCGAGCTAGTCACCGTGCCGGAGGAGGTAGAAATCGCCGGGAAATTGCCGGCGGAGACTGGGGTCTACGCCGTTTTTGACCAGAGCGGAGAGCTGCAGTTCGTCGGGTTATCGAGGAACATAGCGGCGAGTATTCTCTCCCACCGGAAGTCGTTGCCGGAGCTTTGCTACTCCGTTAAGGTTGGGGTAGTAGATGAACCAGATAGGGCGGTTCTAACGGAAGCTTGGAAATCATGGATGGAAGAACACATAAAAGCAACTGGGAAGGTTCCCCCGGGAAATGAATCGGGCAACGCCACATGGGTCCGAAAGCCACCACCGAGGAAGAAGAAGTCTGATCTTCGGCTAACACCTGGTCCTAATGTGCAGCTGACAGTGCCACTGGAGGAACTCATTGACCGGTTGGTGAATGAGAACAAAGTTGTGGCCTTCATCAAGGGTTCGAGAAGTGCCCCATTGTGTGGTTTCTCGCAGAAGGTTGTCGGAATTCTTGAAAACCACGGGGTGGATTATGAAAGCGTTGATGTGCTTGACGAAGAGTATAATAGAGGATTGAGGGAGACTCTGAAGAAGTACAGTAACTGGCCGACTTTCCCCCAGATATTCGTGAATGGAGAACTGCTTGGGGGATGTGATATCCTAAGTTCCATGCAGGAGAAGGGCGAGCTTGCCAGCTTGTTTAAAAAGTGA

Protein Analysis

299

Amino Acids

32.66

Weight (kDa)

8.31

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 210 - 276 4.1e-18 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38270
fragaria_vesca FvH4_6g11240
malus_domestica MD00G1172400.v1.1
prunus_persica Prupe.6G279600_v2.0.a1
pyrus_communis pycom12g16270
rosa_chinensis RchiOBHm_Chr3g0462751
rosa_laevigata RLG00000024835
rosa_multiflora Rmu_co8134540.1_g000001 Rmu_sc0000766.1_g000004
rosa_roxburghii Rroxscaffold_6G00417230
rosa_rugosa Rorug03G0059000
rosa_samantha Rh3AG117000 Rh3BG120700 Rh3CG123700 Rh3DG122400
rosa_wichuraiana Rw3G009910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 668, 712
AccBSI CCGCTC 2 cut(s) 129, 294
AccI GTMKAC 1 cut(s) 273
AciI CCGC 7 cut(s) 52, 127, 153, 260, 294, 332, 416
AcoI YGGCCR 1 cut(s) 791
AcsI RAATTY 1 cut(s) 693
AcuI CTGAAG 1 cut(s) 794
AdeI CACNNNGTG 1 cut(s) 712
AfaI GTAC 1 cut(s) 782
AfiI CCNNNNNNNGG 3 cut(s) 172, 668, 712
AgeI ACCGGT 1 cut(s) 606
AgsI TTSAA 2 cut(s) 100, 701
AjnI CCWGG 1 cut(s) 559
AjuI GAANNNNNNNTTGG 4 cut(s) 19, 51, 80, 112
AluBI AGCT 8 cut(s) 170, 214, 301, 370, 431, 577, 874, 882
AluI AGCT 8 cut(s) 170, 214, 301, 370, 431, 577, 874, 882
Alw26I GTCTC 3 cut(s) 200, 257, 761
AlwNI CAGNNNCTG 1 cut(s) 789
Ama87I CYCGRG 2 cut(s) 165, 481
AoxI GGCC 3 cut(s) 195, 633, 791
ApeKI GCWGC 2 cut(s) 301, 574
ApoI RAATTY 1 cut(s) 693
AsiGI ACCGGT 1 cut(s) 606
AspS9I GGNCC 3 cut(s) 196, 511, 563
AsuC2I CCSGG 3 cut(s) 247, 482, 483
AsuHPI GGTGA 2 cut(s) 211, 625
AvaI CYCGRG 2 cut(s) 165, 481
AvaII GGWCC 2 cut(s) 511, 563
BaeGI GKGCMC 1 cut(s) 661
BbvI GCAGC 2 cut(s) 288, 586
BccI CCATC 1 cut(s) 439
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 561
BcnI CCSGG 3 cut(s) 247, 482, 483
BcoDI GTCTC 3 cut(s) 200, 257, 761
BfaI CTAG 2 cut(s) 48, 215
BfmI CTRYAG 1 cut(s) 302
BisI GCNGC 5 cut(s) 52, 153, 302, 333, 575
BlsI GCNGC 5 cut(s) 53, 154, 303, 334, 576
Bme1390I CCNGG 4 cut(s) 247, 482, 483, 561
Bme18I GGWCC 2 cut(s) 511, 563
BmeT110I CYCGRG 2 cut(s) 165, 481
BmgT120I GGNCC 3 cut(s) 196, 511, 563
BmiI GGNNCC 2 cut(s) 477, 512
BmrFI CCNGG 4 cut(s) 247, 482, 483, 561
BmrI ACTGGG 2 cut(s) 276, 477
BmsI GCATC 1 cut(s) 164
BmuI ACTGGG 2 cut(s) 276, 477
BoxI GACNNNNGTC 1 cut(s) 269
BplI GAGNNNNNCTC 2 cut(s) 754, 786
BpmI CTGGAG 1 cut(s) 611
BpuMI CCSGG 3 cut(s) 247, 482, 483
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 4 cut(s) 480, 481, 527, 706
BsaWI WCCGGW 2 cut(s) 351, 606
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 668, 712
Bse118I RCCGGY 2 cut(s) 256, 606
Bse1I ACTGG 4 cut(s) 271, 472, 594, 794
BseBI CCWGG 1 cut(s) 561
BseDI CCNNGG 4 cut(s) 480, 481, 527, 706
BseGI GGATG 4 cut(s) 12, 155, 450, 842
BseLI CCNNNNNNNGG 3 cut(s) 172, 668, 712
BseNI ACTGG 4 cut(s) 271, 472, 594, 794
BseRI GAGGAG 1 cut(s) 245
BseSI GKGCMC 1 cut(s) 661
BseXI GCAGC 2 cut(s) 288, 586
BsgI GTGCAG 1 cut(s) 593
BshFI GGCC 3 cut(s) 197, 635, 793
BshTI ACCGGT 1 cut(s) 606
BsiHKCI CYCGRG 2 cut(s) 165, 481
BsiSI CCGG 7 cut(s) 227, 246, 257, 352, 365, 482, 607
BslI CCNNNNNNNGG 3 cut(s) 172, 668, 712
BsmAI GTCTC 3 cut(s) 200, 257, 761
BsnI GGCC 3 cut(s) 197, 635, 793
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 2 cut(s) 165, 481
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 1 cut(s) 544
BspACI CCGC 7 cut(s) 52, 127, 153, 260, 294, 332, 416
BspANI GGCC 3 cut(s) 197, 635, 793
BspLI GGNNCC 2 cut(s) 477, 512
BspMAI CTGCAG 1 cut(s) 306
BspTNI GGTCTC 1 cut(s) 200
BsrBI CCGCTC 2 cut(s) 129, 294
BsrFI RCCGGY 2 cut(s) 256, 606
BsrI ACTGG 4 cut(s) 271, 472, 594, 794
BssAI RCCGGY 2 cut(s) 256, 606
BssECI CCNNGG 4 cut(s) 480, 481, 527, 706
BssMI GATC 1 cut(s) 544
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 3 cut(s) 223, 583, 785
Bst6I CTCTTC 2 cut(s) 67, 738
BstC8I GCNNGC 4 cut(s) 258, 872, 876, 880
BstDEI CTNAG 1 cut(s) 848
BstDSI CCRYGG 1 cut(s) 706
BstF5I GGATG 4 cut(s) 12, 155, 450, 842
BstKTI GATC 1 cut(s) 547
BstMAI GTCTC 3 cut(s) 200, 257, 761
BstMBI GATC 1 cut(s) 544
BstMWI GCNNNNNNNGC 2 cut(s) 194, 583
BstNI CCWGG 1 cut(s) 561
BstPAI GACNNNNGTC 1 cut(s) 269
BstSCI CCNGG 4 cut(s) 245, 480, 481, 559
BstSFI CTRYAG 1 cut(s) 302
BstSLI GKGCMC 1 cut(s) 661
BstV1I GCAGC 2 cut(s) 288, 586
BsuRI GGCC 3 cut(s) 197, 635, 793
BtgI CCRYGG 1 cut(s) 706
BtsCI GGATG 4 cut(s) 12, 155, 450, 842
BtsIMutI CAGTG 2 cut(s) 587, 588
Cac8I GCNNGC 4 cut(s) 258, 872, 876, 880
CaiI CAGNNNCTG 1 cut(s) 789
Cfr10I RCCGGY 2 cut(s) 256, 606
Cfr13I GGNCC 3 cut(s) 196, 511, 563
Cfr9I CCCGGG 1 cut(s) 481
CsiI ACCWGGT 1 cut(s) 559
Csp6I GTAC 1 cut(s) 781
CspAI ACCGGT 1 cut(s) 606
CviAII CATG 3 cut(s) 441, 507, 856
CviQI GTAC 1 cut(s) 781
DdeI CTNAG 1 cut(s) 848
DpnI GATC 1 cut(s) 546
DpnII GATC 1 cut(s) 544
DraI TTTAAA 1 cut(s) 889
DraIII CACNNNGTG 1 cut(s) 712
EaeI YGGCCR 1 cut(s) 791
Eam1104I CTCTTC 2 cut(s) 67, 738
EarI CTCTTC 2 cut(s) 67, 738
EciI GGCGGA 1 cut(s) 275
Eco31I GGTCTC 1 cut(s) 200
Eco32I GATATC 1 cut(s) 844
Eco47I GGWCC 2 cut(s) 511, 563
Eco57I CTGAAG 1 cut(s) 794
Eco88I CYCGRG 2 cut(s) 165, 481
EcoO109I RGGNCCY 1 cut(s) 196
EcoRI GAATTC 1 cut(s) 693
EcoRII CCWGG 1 cut(s) 559
EcoRV GATATC 1 cut(s) 844
FaeI CATG 3 cut(s) 444, 510, 859
FaiI YATR 7 cut(s) 329, 442, 458, 508, 720, 750, 857
FatI CATG 3 cut(s) 440, 506, 855
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 5 cut(s) 52, 153, 302, 333, 575
FokI GGATG 3 cut(s) 142, 457, 849
Fsp4HI GCNGC 5 cut(s) 52, 153, 302, 333, 575
FspBI CTAG 2 cut(s) 48, 215
GluI GCNGC 5 cut(s) 52, 153, 302, 333, 575
GsuI CTGGAG 1 cut(s) 611
HaeIII GGCC 3 cut(s) 197, 635, 793
HapII CCGG 7 cut(s) 227, 246, 257, 352, 365, 482, 607
Hin1II CATG 3 cut(s) 444, 510, 859
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 2 cut(s) 491, 769
HpaII CCGG 7 cut(s) 227, 246, 257, 352, 365, 482, 607
HphI GGTGA 2 cut(s) 211, 625
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 5 cut(s) 205, 515, 544, 692, 774
Hpy188III TCNNGA 3 cut(s) 651, 698, 814
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 5 cut(s) 133, 466, 646, 676, 859
HpyCH4III ACNGT 3 cut(s) 223, 583, 785
HpyCH4V TGCA 3 cut(s) 304, 574, 859
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 583
HpyF3I CTNAG 1 cut(s) 848
Hsp92II CATG 3 cut(s) 444, 510, 859
KroI GCCGGC 1 cut(s) 256
KroNI GCCGGC 1 cut(s) 258
Kzo9I GATC 1 cut(s) 544
LmnI GCTCC 2 cut(s) 134, 367
Lsp1109I GCAGC 2 cut(s) 288, 586
LweI GCATC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 559
MaeI CTAG 2 cut(s) 48, 215
MaeIII GTNAC 2 cut(s) 217, 785
MalI GATC 1 cut(s) 546
MbiI CCGCTC 2 cut(s) 129, 294
MboI GATC 1 cut(s) 544
MboII GAAGA 7 cut(s) 54, 461, 539, 544, 547, 755, 787
MhlI GDGCHC 1 cut(s) 661
MluCI AATT 3 cut(s) 100, 251, 693
MlyI GAGTC 1 cut(s) 763
MmeI TCCRAC 1 cut(s) 670
MroNI GCCGGC 1 cut(s) 256
MseI TTAA 3 cut(s) 192, 384, 888
MspA1I CMGCKG 1 cut(s) 577
MspI CCGG 7 cut(s) 227, 246, 257, 352, 365, 482, 607
MspR9I CCNGG 4 cut(s) 247, 482, 483, 561
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 2 cut(s) 194, 583
NaeI GCCGGC 1 cut(s) 258
NciI CCSGG 3 cut(s) 247, 482, 483
NdeII GATC 1 cut(s) 544
NgoMIV GCCGGC 1 cut(s) 256
NlaIII CATG 3 cut(s) 444, 510, 859
NlaIV GGNNCC 2 cut(s) 477, 512
NmuCI GTSAC 1 cut(s) 217
PdiI GCCGGC 1 cut(s) 258
PfeI GAWTC 1 cut(s) 491
PflMI CCANNNNNTGG 2 cut(s) 668, 712
PinAI ACCGGT 1 cut(s) 606
PkrI GCNGC 5 cut(s) 53, 154, 303, 334, 576
PleI GAGTC 1 cut(s) 763
PpsI GAGTC 1 cut(s) 763
PshAI GACNNNNGTC 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 2 cut(s) 477, 512
PspPI GGNCC 3 cut(s) 196, 511, 563
PstI CTGCAG 1 cut(s) 306
PstNI CAGNNNCTG 1 cut(s) 789
PvuII CAGCTG 1 cut(s) 577
RsaI GTAC 1 cut(s) 782
RsaNI GTAC 1 cut(s) 781
SaqAI TTAA 3 cut(s) 192, 384, 888
SatI GCNGC 5 cut(s) 52, 153, 302, 333, 575
Sau3AI GATC 1 cut(s) 544
Sau96I GGNCC 3 cut(s) 196, 511, 563
SchI GAGTC 1 cut(s) 763
ScrFI CCNGG 4 cut(s) 247, 482, 483, 561
SduI GDGCHC 1 cut(s) 661
SexAI ACCWGGT 1 cut(s) 559
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 302
SinI GGWCC 2 cut(s) 511, 563
SmaI CCCGGG 1 cut(s) 483
Sse9I AATT 3 cut(s) 100, 251, 693
SsiI CCGC 7 cut(s) 52, 127, 153, 260, 294, 332, 416
SspMI CTAG 2 cut(s) 48, 215
StyD4I CCNGG 4 cut(s) 245, 480, 481, 559
TaaI ACNGT 3 cut(s) 223, 583, 785
TaqI TCGA 2 cut(s) 320, 650
TaqII GACCGA 2 cut(s) 163, 224
TasI AATT 3 cut(s) 100, 251, 693
TatI WGTACW 1 cut(s) 780
TauI GCSGC 3 cut(s) 54, 155, 335
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 3 cut(s) 192, 384, 888
Tru9I TTAA 3 cut(s) 192, 384, 888
TscAI CASTG 2 cut(s) 588, 594
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 2 cut(s) 301, 574
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 5 cut(s) 27, 417, 504, 628, 735
TspGWI ACGGA 2 cut(s) 370, 440
TspMI CCCGGG 1 cut(s) 481
TspRI CASTG 2 cut(s) 588, 594
Van91I CCANNNNNTGG 2 cut(s) 668, 712
VpaK11BI GGWCC 2 cut(s) 511, 563
XapI RAATTY 1 cut(s) 693
XmaI CCCGGG 1 cut(s) 481
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 2 cut(s) 48, 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.