Rroxscaffold_6G00417230

Monothiol glutaredoxin-S16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015118
Physical Location & Seq
Reverse (-)
39010618 .. 39012807
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00417230.1

Sequence Viewer

Length: 891 bp
ATGGCCACAATCATCCACCTCTCTCCGATTCACACTTCGCCGCCGTCTCCTCGCTTATTTTCTCCCCAATCTTCCCAAAATACCCTTCACCTCTCATTCAATTCACACCCCAAACCCCTCTCCCTTCCCTCCATTTCTCTCAAGCCCTGCGCTCCGGCCAGGCCTCGAGCTTTGGTCGTCACTTCGGCTGTTAAGAGCCTCTCGGAGACTGAGCTAGTCTCAGTGGAGGGCGAACAAGTCGCTGGAAAATTACCGTCGGAGACCGGCGTCTACGCCGTTTTTGACCAGAACGGTGAGCTTCAGTTCGTCGGCTTATCGAGAAACATAGCGGCGAGTGTTCTTGTTCACCGGAAATCGGTGCCGGAGCTGTGCCACTCAGTCAAGGTTGGGATAGTAGATGAACCAGATAGGACGGTTCTAACTGAAGCTTGGAAATCATGGATGGAAGAACACATAAAAGCTACTGGGAAAGTGCCTCCAGGAAATGAATCAGGCAACGCCACCTGGGTCCGGCAGGCTCCTAAAAAGAAGAAGCCTGATCTGCGGCTGACACCAGGTCGTCACGTGCAGTTGACGGTTCCATTGGAGCAACTTGTGGATCGGTTGGTGAAGGAGAACAAGGTCGTGGCCTTTATCAAGGGTTCACGAAGTGCACCATTGTGTGGTTTCTCGCAGAGGGTTGTTGGCATTCTTGAAAATCAAGGGGTGGATTATGAGAGCGTTGATGTGCTTGACGAAGAGTATAACTCTGGATTGAGGGAGACTCTCAAGACCTATAGTAATTGGCCTACTTTCCCGCAGATATTTGTGAATGGAGAACTGGTTGGGGGTTGCGATATTCTAACATCGATGCACGAAAAGGGAGAGCTTGCTAGCTTGCTTAAGAAATGA

Protein Analysis

296

Amino Acids

32.39

Weight (kDa)

8.28

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 208 - 275 9.5e-18 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38270
fragaria_vesca FvH4_6g11240
malus_domestica MD00G1172400.v1.1
prunus_persica Prupe.6G279600_v2.0.a1
pyrus_communis pycom12g16270
rosa_chinensis RchiOBHm_Chr3g0462751
rosa_laevigata RLG00000024835
rosa_multiflora Rmu_co8134540.1_g000001 Rmu_sc0000766.1_g000004
rosa_roxburghii Rroxscaffold_6G00417230
rosa_rugosa Rorug03G0059000
rosa_samantha Rh3AG117000 Rh3BG120700 Rh3CG123700 Rh3DG122400
rosa_wichuraiana Rw3G009910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 662
AccI GTMKAC 1 cut(s) 270
AciI CCGC 4 cut(s) 41, 329, 544, 797
AclWI GGATC 1 cut(s) 606
AcoI YGGCCR 2 cut(s) 3, 156
AcuI CTGAAG 2 cut(s) 284, 444
AcvI CACGTG 1 cut(s) 565
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 650
AfiI CCNNNNNNNGG 3 cut(s) 355, 510, 662
AflII CTTAAG 1 cut(s) 881
AgsI TTSAA 2 cut(s) 100, 695
AhdI GACNNNNNGTC 1 cut(s) 555
AjnI CCWGG 4 cut(s) 158, 478, 503, 553
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AleI CACNNNNGTG 1 cut(s) 658
AluBI AGCT 8 cut(s) 170, 214, 298, 367, 428, 461, 868, 876
AluI AGCT 8 cut(s) 170, 214, 298, 367, 428, 461, 868, 876
Alw21I GWGCWC 1 cut(s) 655
Alw26I GTCTC 5 cut(s) 51, 200, 223, 254, 755
Alw44I GTGCAC 1 cut(s) 651
AlwI GGATC 1 cut(s) 606
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 5 cut(s) 3, 156, 161, 627, 785
ApaLI GTGCAC 1 cut(s) 651
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 4 cut(s) 80, 305, 338, 619
AsuNHI GCTAGC 1 cut(s) 872
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 1 cut(s) 508
BaeGI GKGCMC 1 cut(s) 655
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 358
BbrPI CACGTG 1 cut(s) 565
Bbv12I GWGCWC 1 cut(s) 655
BccI CCATC 1 cut(s) 436
BceAI ACGGC 2 cut(s) 28, 260
BciT130I CCWGG 4 cut(s) 160, 480, 505, 555
BcoDI GTCTC 5 cut(s) 51, 200, 223, 254, 755
BfaI CTAG 2 cut(s) 215, 873
BfmI CTRYAG 1 cut(s) 775
BfrI CTTAAG 1 cut(s) 881
BisI GCNGC 3 cut(s) 41, 330, 545
BlsI GCNGC 3 cut(s) 42, 331, 546
Bme1390I CCNGG 4 cut(s) 160, 480, 505, 555
Bme18I GGWCC 1 cut(s) 508
BmeRI GACNNNNNGTC 1 cut(s) 555
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 4 cut(s) 360, 509, 519, 579
BmrFI CCNGG 4 cut(s) 160, 480, 505, 555
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 1 cut(s) 840
BmtI GCTAGC 1 cut(s) 876
BmuI ACTGGG 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 266
BplI GAGNNNNNCTC 6 cut(s) 203, 235, 731, 763, 748, 780
BpmI CTGGAG 1 cut(s) 462
BpuEI CTTGAG 2 cut(s) 125, 752
Bsa29I ATCGAT 1 cut(s) 848
BsaAI YACGTR 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 504
BsaWI WCCGGW 1 cut(s) 348
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 3 cut(s) 355, 510, 662
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 469, 825
BseBI CCWGG 4 cut(s) 160, 480, 505, 555
BseCI ATCGAT 1 cut(s) 848
BseDI CCNNGG 1 cut(s) 504
BseGI GGATG 2 cut(s) 12, 447
BseLI CCNNNNNNNGG 3 cut(s) 355, 510, 662
BseMII CTCAG 3 cut(s) 201, 234, 390
BseNI ACTGG 2 cut(s) 469, 825
BseRI GAGGAG 1 cut(s) 39
BseSI GKGCMC 1 cut(s) 655
BsgI GTGCAG 1 cut(s) 587
BshFI GGCC 5 cut(s) 5, 158, 163, 629, 787
BshNI GGYRCC 1 cut(s) 358
BshVI ATCGAT 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 655
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 5 cut(s) 155, 264, 349, 362, 511
BslI CCNNNNNNNGG 3 cut(s) 355, 510, 662
BsmAI GTCTC 5 cut(s) 51, 200, 223, 254, 755
BsmBI CGTCTC 1 cut(s) 51
BsmI GAATGC 1 cut(s) 687
BsnI GGCC 5 cut(s) 5, 158, 163, 629, 787
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 655
Bsp143I GATC 2 cut(s) 538, 598
BspACI CCGC 4 cut(s) 41, 329, 544, 797
BspANI GGCC 5 cut(s) 5, 158, 163, 629, 787
BspCNI CTCAG 3 cut(s) 202, 233, 389
BspDI ATCGAT 1 cut(s) 848
BspLI GGNNCC 4 cut(s) 360, 509, 519, 579
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 1 cut(s) 606
BspT107I GGYRCC 1 cut(s) 358
BspTI CTTAAG 1 cut(s) 881
BspTNI GGTCTC 1 cut(s) 254
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 2 cut(s) 469, 825
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 2 cut(s) 538, 598
BssNI GRCGYC 1 cut(s) 267
Bst2UI CCWGG 4 cut(s) 160, 480, 505, 555
Bst4CI ACNGT 4 cut(s) 255, 293, 415, 577
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 1 cut(s) 267
BstAFI CTTAAG 1 cut(s) 881
BstBAI YACGTR 1 cut(s) 565
BstC8I GCNNGC 4 cut(s) 516, 870, 874, 878
BstDEI CTNAG 3 cut(s) 210, 220, 376
BstF5I GGATG 2 cut(s) 12, 447
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 2 cut(s) 541, 601
BstMAI GTCTC 5 cut(s) 51, 200, 223, 254, 755
BstMBI GATC 2 cut(s) 538, 598
BstMWI GCNNNNNNNGC 1 cut(s) 541
BstNI CCWGG 4 cut(s) 160, 480, 505, 555
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 4 cut(s) 158, 478, 503, 553
BstSFI CTRYAG 1 cut(s) 775
BstSLI GKGCMC 1 cut(s) 655
Bsu15I ATCGAT 1 cut(s) 848
BsuRI GGCC 5 cut(s) 5, 158, 163, 629, 787
BsuTUI ATCGAT 1 cut(s) 848
BtsCI GGATG 2 cut(s) 12, 447
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 4 cut(s) 516, 870, 874, 878
CfoI GCGC 1 cut(s) 152
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 508
ClaI ATCGAT 1 cut(s) 848
CseI GACGC 1 cut(s) 256
CsiI ACCWGGT 1 cut(s) 553
CviAII CATG 1 cut(s) 438
DdeI CTNAG 3 cut(s) 210, 220, 376
DpnI GATC 2 cut(s) 540, 600
DpnII GATC 2 cut(s) 538, 598
DraIII CACNNNGTG 1 cut(s) 650
DriI GACNNNNNGTC 1 cut(s) 555
EaeI YGGCCR 2 cut(s) 3, 156
Eam1104I CTCTTC 1 cut(s) 732
Eam1105I GACNNNNNGTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 732
Eco147I AGGCCT 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 508
Eco57I CTGAAG 2 cut(s) 284, 444
Eco72I CACGTG 1 cut(s) 565
Eco88I CYCGRG 1 cut(s) 165
EcoRII CCWGG 4 cut(s) 158, 478, 503, 553
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 1 cut(s) 441
FaiI YATR 6 cut(s) 326, 439, 455, 714, 744, 777
FatI CATG 1 cut(s) 437
FauI CCCGC 1 cut(s) 804
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 3 cut(s) 41, 330, 545
FokI GGATG 1 cut(s) 454
Fsp4HI GCNGC 3 cut(s) 41, 330, 545
FspBI CTAG 2 cut(s) 215, 873
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 41, 330, 545
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 5 cut(s) 5, 158, 163, 629, 787
HapII CCGG 5 cut(s) 155, 264, 349, 362, 511
HgaI GACGC 1 cut(s) 256
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 1 cut(s) 441
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 3 cut(s) 28, 488, 763
HpaII CCGG 5 cut(s) 155, 264, 349, 362, 511
HphI GGTGA 4 cut(s) 80, 305, 338, 619
Hpy166II GTNNAC 5 cut(s) 271, 346, 573, 644, 653
Hpy188I TCNGA 3 cut(s) 27, 205, 259
Hpy188III TCNNGA 5 cut(s) 318, 645, 692, 750, 769
Hpy8I GTNNAC 5 cut(s) 271, 346, 573, 644, 653
Hpy99I CGWCG 2 cut(s) 259, 311
HpyAV CCTTC 3 cut(s) 95, 134, 604
HpyCH4III ACNGT 4 cut(s) 255, 293, 415, 577
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 3 cut(s) 568, 653, 853
HpyF10VI GCNNNNNNNGC 1 cut(s) 541
HpyF3I CTNAG 3 cut(s) 210, 220, 376
HpySE526I ACGT 1 cut(s) 564
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 1 cut(s) 441
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 2 cut(s) 538, 598
LmnI GCTCC 4 cut(s) 157, 364, 523, 586
LweI GCATC 1 cut(s) 840
MabI ACCWGGT 1 cut(s) 553
MaeI CTAG 2 cut(s) 215, 873
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 2 cut(s) 178, 560
MalI GATC 2 cut(s) 540, 600
MboI GATC 2 cut(s) 538, 598
MboII GAAGA 4 cut(s) 63, 458, 541, 749
MhlI GDGCHC 1 cut(s) 655
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 100, 248, 781
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 757
MmeI TCCRAC 1 cut(s) 237
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 192, 882
MslI CAYNNNNRTG 1 cut(s) 658
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 881
MspI CCGG 5 cut(s) 155, 264, 349, 362, 511
MspR9I CCNGG 4 cut(s) 160, 480, 505, 555
Mva1269I GAATGC 1 cut(s) 687
MvaI CCWGG 4 cut(s) 160, 480, 505, 555
MwoI GCNNNNNNNGC 1 cut(s) 541
NdeII GATC 2 cut(s) 538, 598
NheI GCTAGC 1 cut(s) 872
NlaIII CATG 1 cut(s) 441
NlaIV GGNNCC 4 cut(s) 360, 509, 519, 579
NmuCI GTSAC 2 cut(s) 178, 560
OliI CACNNNNGTG 1 cut(s) 658
PaeR7I CTCGAG 1 cut(s) 165
PceI AGGCCT 1 cut(s) 163
PctI GAATGC 1 cut(s) 687
PfeI GAWTC 2 cut(s) 28, 488
PflMI CCANNNNNTGG 1 cut(s) 662
PfoI TCCNGGA 1 cut(s) 478
PkrI GCNGC 3 cut(s) 42, 331, 546
PleI GAGTC 1 cut(s) 757
PmaCI CACGTG 1 cut(s) 565
PmlI CACGTG 1 cut(s) 565
PpsI GAGTC 1 cut(s) 757
Ppu21I YACGTR 1 cut(s) 565
PshAI GACNNNNGTC 1 cut(s) 266
Psp6I CCWGG 4 cut(s) 158, 478, 503, 553
PspCI CACGTG 1 cut(s) 565
PspGI CCWGG 4 cut(s) 158, 478, 503, 553
PspN4I GGNNCC 4 cut(s) 360, 509, 519, 579
PspPI GGNCC 1 cut(s) 508
PspXI VCTCGAGB 1 cut(s) 165
RseI CAYNNNNRTG 1 cut(s) 658
SaqAI TTAA 2 cut(s) 192, 882
SatI GCNGC 3 cut(s) 41, 330, 545
Sau3AI GATC 2 cut(s) 538, 598
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 1 cut(s) 757
ScrFI CCNGG 4 cut(s) 160, 480, 505, 555
SduI GDGCHC 1 cut(s) 655
SexAI ACCWGGT 1 cut(s) 553
SfaNI GCATC 1 cut(s) 840
SfcI CTRYAG 1 cut(s) 775
Sfr274I CTCGAG 1 cut(s) 165
SinI GGWCC 1 cut(s) 508
SlaI CTCGAG 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 4 cut(s) 140, 165, 767, 881
SmoI CTYRAG 4 cut(s) 140, 165, 767, 881
Sse9I AATT 3 cut(s) 100, 248, 781
SseBI AGGCCT 1 cut(s) 163
SsiI CCGC 4 cut(s) 41, 329, 544, 797
SspMI CTAG 2 cut(s) 215, 873
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 4 cut(s) 158, 478, 503, 553
TaaI ACNGT 4 cut(s) 255, 293, 415, 577
TaiI ACGT 1 cut(s) 567
TaqI TCGA 3 cut(s) 166, 317, 848
TasI AATT 3 cut(s) 100, 248, 781
TauI GCSGC 3 cut(s) 43, 332, 547
TfiI GAWTC 2 cut(s) 28, 488
Tru1I TTAA 2 cut(s) 192, 882
Tru9I TTAA 2 cut(s) 192, 882
TscAI CASTG 1 cut(s) 228
TseFI GTSAC 2 cut(s) 178, 560
Tsp45I GTSAC 2 cut(s) 178, 560
TspDTI ATGAA 2 cut(s) 414, 501
TspRI CASTG 1 cut(s) 228
Van91I CCANNNNNTGG 1 cut(s) 662
Vha464I CTTAAG 1 cut(s) 881
VneI GTGCAC 1 cut(s) 651
VpaK11BI GGWCC 1 cut(s) 508
XhoI CTCGAG 1 cut(s) 165
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 2 cut(s) 215, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.