Rw3G009910

Monothiol glutaredoxin-S16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0015118
Physical Location & Seq
Forward (+)
9516025 .. 9518202
2178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G009910.1

Sequence Viewer

Length: 891 bp
ATGGCCACAATCATCCACCTCTCTCCGATTCACACTTCGCCGCCGTCTCCTCGCTTATTTTCTCCCCAATCTTCCCAAAATACCCTTCACCTCTCATTCAATTCACACCCCAAACCCCTCTCCCTTCCCTCCATTTCTCTCAAGCCCTGCGCTCCGGCCAGGCCTCGCGCTTTGGTCGTCACTTCGGCTGTTAAGAGCCTCTCGGAGACTGAGCTAGTCTCAGTGGAGGGCGAACAAGTCGCCGGAAAATTGCCGTCGGAGACCGGCGTCTACGCTGTATTTGACCAGAACGGTGAGCTTCAGTTCGTCGGCTTATCGAGAAACATAGCCGCGAGTGTTCTTGTTCACCGGAAATCGGTGCCGGAGCTGTGCCACTCCGTCAAGGTTGGGATAGTAGATGAACCAGATAGGACGGTTCTAACTGAAGCTTGGAAATCATGGATGGAAGAACACATAAAAGCTACTGGGAAAGTGCCTCCAGGAAATGAATCAGGCAACGCCACCTGGGTCCGGCCGGCACCTAAGAAGAAGAAGCCTGATCTGCGGCTGACACCAGGTCGTCATGTGCAGTTGACGGTTCCATTGGAGCAACTTGTGGATCGGTTGGTGAAGGAGAACAAGGTCGTGGCCTTTATCAAGGGTTCACGAAGTGCACCATTGTGTGGTTTCTCGCAGAGGGTTGTTGGCATTCTTGAAAATCAAGGGGTGGATTATGAGAGCGTTGATGTACTTGATGAAGAGTATAATTCCGGATTGAGGGAGACTCTCAAGACCTATAGTAATTGGCCTACTTTCCCGCAGATATTTGTGAATGGAGAACTGGTTGGGGGTTGCGATATTCTAACATCCATGCACGAAAAGGGAGAGCTTGCTAGCTTGCTTAAGAAATGA

Protein Analysis

296

Amino Acids

32.36

Weight (kDa)

8.28

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 208 - 275 9.5e-18 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38270
fragaria_vesca FvH4_6g11240
malus_domestica MD00G1172400.v1.1
prunus_persica Prupe.6G279600_v2.0.a1
pyrus_communis pycom12g16270
rosa_chinensis RchiOBHm_Chr3g0462751
rosa_laevigata RLG00000024835
rosa_multiflora Rmu_co8134540.1_g000001 Rmu_sc0000766.1_g000004
rosa_roxburghii Rroxscaffold_6G00417230
rosa_rugosa Rorug03G0059000
rosa_samantha Rh3AG117000 Rh3BG120700 Rh3CG123700 Rh3DG122400
rosa_wichuraiana Rw3G009910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 358, 517
AccB7I CCANNNNNTGG 1 cut(s) 662
AccI GTMKAC 1 cut(s) 270
AccII CGCG 2 cut(s) 168, 332
AccIII TCCGGA 1 cut(s) 749
AciI CCGC 4 cut(s) 41, 330, 544, 797
AclWI GGATC 1 cut(s) 606
AcoI YGGCCR 3 cut(s) 3, 156, 512
AcuI CTGAAG 2 cut(s) 284, 444
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 650
AfaI GTAC 1 cut(s) 729
AfiI CCNNNNNNNGG 4 cut(s) 355, 510, 662, 756
AflII CTTAAG 1 cut(s) 881
AgsI TTSAA 2 cut(s) 100, 695
AhdI GACNNNNNGTC 1 cut(s) 555
AjnI CCWGG 4 cut(s) 158, 478, 503, 553
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AleI CACNNNNGTG 1 cut(s) 658
AluBI AGCT 7 cut(s) 214, 298, 367, 428, 461, 868, 876
AluI AGCT 7 cut(s) 214, 298, 367, 428, 461, 868, 876
Alw21I GWGCWC 1 cut(s) 655
Alw26I GTCTC 5 cut(s) 51, 200, 223, 254, 755
Alw44I GTGCAC 1 cut(s) 651
AlwI GGATC 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 749
AoxI GGCC 6 cut(s) 3, 156, 161, 512, 627, 785
ApaLI GTGCAC 1 cut(s) 651
AspLEI GCGC 2 cut(s) 152, 170
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 4 cut(s) 80, 305, 338, 619
AsuNHI GCTAGC 1 cut(s) 872
AvaII GGWCC 1 cut(s) 508
BaeGI GKGCMC 1 cut(s) 655
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 358, 517
Bbv12I GWGCWC 1 cut(s) 655
BccI CCATC 1 cut(s) 436
BceAI ACGGC 2 cut(s) 28, 238
BciT130I CCWGG 4 cut(s) 160, 480, 505, 555
BcoDI GTCTC 5 cut(s) 51, 200, 223, 254, 755
BfaI CTAG 2 cut(s) 215, 873
BfmI CTRYAG 1 cut(s) 775
BfrI CTTAAG 1 cut(s) 881
BisI GCNGC 3 cut(s) 41, 330, 545
BlsI GCNGC 3 cut(s) 42, 331, 546
Bme1390I CCNGG 4 cut(s) 160, 480, 505, 555
Bme18I GGWCC 1 cut(s) 508
BmeRI GACNNNNNGTC 1 cut(s) 555
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 4 cut(s) 360, 509, 519, 579
BmrFI CCNGG 4 cut(s) 160, 480, 505, 555
BmrI ACTGGG 1 cut(s) 474
BmtI GCTAGC 1 cut(s) 876
BmuI ACTGGG 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 266
BplI GAGNNNNNCTC 4 cut(s) 203, 235, 748, 780
BpmI CTGGAG 1 cut(s) 462
BpuEI CTTGAG 2 cut(s) 125, 752
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 504
BsaWI WCCGGW 2 cut(s) 348, 749
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 510, 662, 756
Bse118I RCCGGY 2 cut(s) 263, 514
Bse1I ACTGG 2 cut(s) 469, 825
BseAI TCCGGA 1 cut(s) 749
BseBI CCWGG 4 cut(s) 160, 480, 505, 555
BseDI CCNNGG 1 cut(s) 504
BseGI GGATG 3 cut(s) 12, 447, 845
BseLI CCNNNNNNNGG 4 cut(s) 355, 510, 662, 756
BseMII CTCAG 2 cut(s) 201, 234
BseNI ACTGG 2 cut(s) 469, 825
BseRI GAGGAG 1 cut(s) 39
BseSI GKGCMC 1 cut(s) 655
BseX3I CGGCCG 1 cut(s) 512
BsgI GTGCAG 1 cut(s) 587
Bsh1236I CGCG 2 cut(s) 168, 332
Bsh1285I CGRYCG 1 cut(s) 515
BshFI GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
BshNI GGYRCC 2 cut(s) 358, 517
BsiEI CGRYCG 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 655
BsiSI CCGG 8 cut(s) 155, 243, 264, 349, 362, 511, 515, 750
BslI CCNNNNNNNGG 4 cut(s) 355, 510, 662, 756
BsmAI GTCTC 5 cut(s) 51, 200, 223, 254, 755
BsmBI CGTCTC 1 cut(s) 51
BsmI GAATGC 1 cut(s) 687
BsnI GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
Bso31I GGTCTC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 655
Bsp13I TCCGGA 1 cut(s) 749
Bsp143I GATC 2 cut(s) 538, 598
BspACI CCGC 4 cut(s) 41, 330, 544, 797
BspANI GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
BspCNI CTCAG 2 cut(s) 202, 233
BspEI TCCGGA 1 cut(s) 749
BspFNI CGCG 2 cut(s) 168, 332
BspLI GGNNCC 4 cut(s) 360, 509, 519, 579
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 1 cut(s) 606
BspT107I GGYRCC 2 cut(s) 358, 517
BspTI CTTAAG 1 cut(s) 881
BspTNI GGTCTC 1 cut(s) 254
BsrFI RCCGGY 2 cut(s) 263, 514
BsrI ACTGG 2 cut(s) 469, 825
BssAI RCCGGY 2 cut(s) 263, 514
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 2 cut(s) 538, 598
BssNI GRCGYC 1 cut(s) 267
Bst2UI CCWGG 4 cut(s) 160, 480, 505, 555
Bst4CI ACNGT 3 cut(s) 293, 415, 577
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 1 cut(s) 267
BstAFI CTTAAG 1 cut(s) 881
BstC8I GCNNGC 4 cut(s) 516, 870, 874, 878
BstDEI CTNAG 3 cut(s) 210, 220, 522
BstF5I GGATG 3 cut(s) 12, 447, 845
BstFNI CGCG 2 cut(s) 168, 332
BstHHI GCGC 2 cut(s) 152, 170
BstKTI GATC 2 cut(s) 541, 601
BstMAI GTCTC 5 cut(s) 51, 200, 223, 254, 755
BstMBI GATC 2 cut(s) 538, 598
BstMCI CGRYCG 1 cut(s) 515
BstMWI GCNNNNNNNGC 1 cut(s) 541
BstNI CCWGG 4 cut(s) 160, 480, 505, 555
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 4 cut(s) 158, 478, 503, 553
BstSFI CTRYAG 1 cut(s) 775
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 2 cut(s) 168, 332
BstZI CGGCCG 1 cut(s) 512
BsuRI GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
BtsCI GGATG 3 cut(s) 12, 447, 845
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 4 cut(s) 516, 870, 874, 878
CfoI GCGC 2 cut(s) 152, 170
Cfr10I RCCGGY 2 cut(s) 263, 514
Cfr13I GGNCC 1 cut(s) 508
CseI GACGC 1 cut(s) 256
CsiI ACCWGGT 1 cut(s) 553
Csp6I GTAC 1 cut(s) 728
CviAII CATG 3 cut(s) 438, 563, 850
CviQI GTAC 1 cut(s) 728
DdeI CTNAG 3 cut(s) 210, 220, 522
DpnI GATC 2 cut(s) 540, 600
DpnII GATC 2 cut(s) 538, 598
DraIII CACNNNGTG 1 cut(s) 650
DriI GACNNNNNGTC 1 cut(s) 555
EaeI YGGCCR 3 cut(s) 3, 156, 512
EagI CGGCCG 1 cut(s) 512
Eam1104I CTCTTC 1 cut(s) 732
Eam1105I GACNNNNNGTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 732
EclXI CGGCCG 1 cut(s) 512
Eco147I AGGCCT 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 508
Eco52I CGGCCG 1 cut(s) 512
Eco57I CTGAAG 2 cut(s) 284, 444
EcoRII CCWGG 4 cut(s) 158, 478, 503, 553
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 3 cut(s) 441, 566, 853
FaiI YATR 8 cut(s) 326, 439, 455, 564, 714, 744, 777, 851
FatI CATG 3 cut(s) 437, 562, 849
FauI CCCGC 1 cut(s) 804
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 3 cut(s) 41, 330, 545
FokI GGATG 2 cut(s) 454, 832
Fsp4HI GCNGC 3 cut(s) 41, 330, 545
FspBI CTAG 2 cut(s) 215, 873
GlaI GCGC 2 cut(s) 151, 169
GluI GCNGC 3 cut(s) 41, 330, 545
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 6 cut(s) 5, 158, 163, 514, 629, 787
HapII CCGG 8 cut(s) 155, 243, 264, 349, 362, 511, 515, 750
HgaI GACGC 1 cut(s) 256
HhaI GCGC 2 cut(s) 152, 170
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 441, 566, 853
Hin6I GCGC 2 cut(s) 150, 168
HinP1I GCGC 2 cut(s) 150, 168
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 3 cut(s) 28, 488, 763
HpaII CCGG 8 cut(s) 155, 243, 264, 349, 362, 511, 515, 750
HphI GGTGA 4 cut(s) 80, 305, 338, 619
Hpy166II GTNNAC 5 cut(s) 271, 346, 573, 644, 653
Hpy188I TCNGA 3 cut(s) 27, 205, 259
Hpy188III TCNNGA 5 cut(s) 318, 645, 692, 750, 769
Hpy8I GTNNAC 5 cut(s) 271, 346, 573, 644, 653
Hpy99I CGWCG 2 cut(s) 259, 311
HpyAV CCTTC 3 cut(s) 95, 134, 604
HpyCH4III ACNGT 3 cut(s) 293, 415, 577
HpyCH4V TGCA 3 cut(s) 568, 653, 853
HpyF10VI GCNNNNNNNGC 1 cut(s) 541
HpyF3I CTNAG 3 cut(s) 210, 220, 522
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 441, 566, 853
HspAI GCGC 2 cut(s) 150, 168
Kpn2I TCCGGA 1 cut(s) 749
KroI GCCGGC 1 cut(s) 514
KroNI GCCGGC 1 cut(s) 516
Kzo9I GATC 2 cut(s) 538, 598
LmnI GCTCC 3 cut(s) 157, 364, 586
MabI ACCWGGT 1 cut(s) 553
MaeI CTAG 2 cut(s) 215, 873
MaeIII GTNAC 1 cut(s) 178
MalI GATC 2 cut(s) 540, 600
MboI GATC 2 cut(s) 538, 598
MboII GAAGA 5 cut(s) 63, 458, 538, 541, 749
MhlI GDGCHC 1 cut(s) 655
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 100, 248, 745, 781
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 757
MmeI TCCRAC 1 cut(s) 237
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 749
MroNI GCCGGC 1 cut(s) 514
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 192, 882
MslI CAYNNNNRTG 1 cut(s) 658
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 881
MspI CCGG 8 cut(s) 155, 243, 264, 349, 362, 511, 515, 750
MspR9I CCNGG 4 cut(s) 160, 480, 505, 555
Mva1269I GAATGC 1 cut(s) 687
MvaI CCWGG 4 cut(s) 160, 480, 505, 555
MvnI CGCG 2 cut(s) 168, 332
MwoI GCNNNNNNNGC 1 cut(s) 541
NaeI GCCGGC 1 cut(s) 516
NdeII GATC 2 cut(s) 538, 598
NgoMIV GCCGGC 1 cut(s) 514
NheI GCTAGC 1 cut(s) 872
NlaIII CATG 3 cut(s) 441, 566, 853
NlaIV GGNNCC 4 cut(s) 360, 509, 519, 579
NmuCI GTSAC 1 cut(s) 178
OliI CACNNNNGTG 1 cut(s) 658
PceI AGGCCT 1 cut(s) 163
PctI GAATGC 1 cut(s) 687
PdiI GCCGGC 1 cut(s) 516
PfeI GAWTC 2 cut(s) 28, 488
PflMI CCANNNNNTGG 1 cut(s) 662
PfoI TCCNGGA 1 cut(s) 478
PkrI GCNGC 3 cut(s) 42, 331, 546
PleI GAGTC 1 cut(s) 757
PpsI GAGTC 1 cut(s) 757
PshAI GACNNNNGTC 1 cut(s) 266
Psp6I CCWGG 4 cut(s) 158, 478, 503, 553
PspGI CCWGG 4 cut(s) 158, 478, 503, 553
PspN4I GGNNCC 4 cut(s) 360, 509, 519, 579
PspPI GGNCC 1 cut(s) 508
RsaI GTAC 1 cut(s) 729
RsaNI GTAC 1 cut(s) 728
RseI CAYNNNNRTG 1 cut(s) 658
SaqAI TTAA 2 cut(s) 192, 882
SatI GCNGC 3 cut(s) 41, 330, 545
Sau3AI GATC 2 cut(s) 538, 598
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 1 cut(s) 757
ScrFI CCNGG 4 cut(s) 160, 480, 505, 555
SduI GDGCHC 1 cut(s) 655
SexAI ACCWGGT 1 cut(s) 553
SfcI CTRYAG 1 cut(s) 775
SinI GGWCC 1 cut(s) 508
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 3 cut(s) 140, 767, 881
SmoI CTYRAG 3 cut(s) 140, 767, 881
Sse9I AATT 4 cut(s) 100, 248, 745, 781
SseBI AGGCCT 1 cut(s) 163
SsiI CCGC 4 cut(s) 41, 330, 544, 797
SspMI CTAG 2 cut(s) 215, 873
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 4 cut(s) 158, 478, 503, 553
TaaI ACNGT 3 cut(s) 293, 415, 577
TaqI TCGA 1 cut(s) 317
TasI AATT 4 cut(s) 100, 248, 745, 781
TatI WGTACW 1 cut(s) 727
TauI GCSGC 3 cut(s) 43, 332, 547
TfiI GAWTC 2 cut(s) 28, 488
Tru1I TTAA 2 cut(s) 192, 882
Tru9I TTAA 2 cut(s) 192, 882
TscAI CASTG 1 cut(s) 228
TseFI GTSAC 1 cut(s) 178
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 3 cut(s) 414, 501, 750
TspGWI ACGGA 1 cut(s) 367
TspRI CASTG 1 cut(s) 228
Van91I CCANNNNNTGG 1 cut(s) 662
Vha464I CTTAAG 1 cut(s) 881
VneI GTGCAC 1 cut(s) 651
VpaK11BI GGWCC 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 2 cut(s) 215, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.