Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G62690 AT5G62700 |
| fragaria_vesca | FvH4_6g12290 |
| malus_domestica | MD04G1143700.v1.1 MD12G1158100.v1.1 |
| prunus_persica | Prupe.6G268300_v2.0.a1 |
| pyrus_communis | pycom04g13060 pycom05g25220 pycom06g07900 pycom07g24790 pycom11g07310 pycom12g15020 |
| rosa_chinensis | RchiOBHm_Chr3g0464121 |
| rosa_laevigata | RLG00000024721 |
| rosa_multiflora | Rmu_sc0010401.1_g000003 |
| rosa_roxburghii | Rroxscaffold_6G00415980 |
| rosa_rugosa | Rorug03G0070700 |
| rosa_wichuraiana | Rw3G010800 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 269 |
| Acc16I | TGCGCA | 1 cut(s) | 904 |
| Acc36I | ACCTGC | 1 cut(s) | 162 |
| AccB1I | GGYRCC | 3 cut(s) | 95, 212, 287 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 60, 665 |
| AccI | GTMKAC | 1 cut(s) | 147 |
| AciI | CCGC | 5 cut(s) | 27, 36, 122, 168, 637 |
| AclWI | GGATC | 3 cut(s) | 146, 207, 439 |
| AcoI | YGGCCR | 3 cut(s) | 28, 244, 277 |
| AcuI | CTGAAG | 2 cut(s) | 110, 238 |
| AcyI | GRCGYC | 1 cut(s) | 288 |
| AfaI | GTAC | 6 cut(s) | 107, 476, 842, 1193, 1274, 1304 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 22, 60, 291, 665, 920, 951, 1076 |
| AgsI | TTSAA | 3 cut(s) | 676, 1084, 1095 |
| AjnI | CCWGG | 4 cut(s) | 21, 208, 727, 1275 |
| AluBI | AGCT | 8 cut(s) | 130, 188, 574, 617, 649, 663, 908, 1289 |
| AluI | AGCT | 8 cut(s) | 130, 188, 574, 617, 649, 663, 908, 1289 |
| Alw21I | GWGCWC | 4 cut(s) | 84, 195, 570, 968 |
| Alw26I | GTCTC | 3 cut(s) | 519, 529, 791 |
| Alw44I | GTGCAC | 1 cut(s) | 566 |
| AlwI | GGATC | 3 cut(s) | 146, 207, 439 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1232 |
| Ama87I | CYCGRG | 1 cut(s) | 203 |
| AoxI | GGCC | 6 cut(s) | 28, 159, 244, 257, 277, 369 |
| ApaLI | GTGCAC | 1 cut(s) | 566 |
| ApeKI | GCWGC | 1 cut(s) | 905 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1178 |
| AspLEI | GCGC | 3 cut(s) | 77, 290, 905 |
| AspS9I | GGNCC | 1 cut(s) | 236 |
| AsuC2I | CCSGG | 2 cut(s) | 292, 479 |
| AsuHPI | GGTGA | 2 cut(s) | 859, 1211 |
| AsuII | TTCGAA | 1 cut(s) | 458 |
| AvaI | CYCGRG | 1 cut(s) | 203 |
| AvaII | GGWCC | 1 cut(s) | 236 |
| AxyI | CCTNAGG | 1 cut(s) | 749 |
| BaeGI | GKGCMC | 1 cut(s) | 570 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 522, 555, 1183, 1216 |
| BanI | GGYRCC | 3 cut(s) | 95, 212, 287 |
| BanII | GRGCYC | 1 cut(s) | 546 |
| BauI | CACGAG | 1 cut(s) | 825 |
| BbsI | GAAGAC | 1 cut(s) | 265 |
| Bbv12I | GWGCWC | 4 cut(s) | 84, 195, 570, 968 |
| BbvCI | CCTCAGC | 1 cut(s) | 1290 |
| BbvI | GCAGC | 1 cut(s) | 917 |
| BccI | CCATC | 2 cut(s) | 1081, 1210 |
| BceAI | ACGGC | 3 cut(s) | 100, 109, 259 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 28, 62 |
| BciT130I | CCWGG | 4 cut(s) | 23, 210, 729, 1277 |
| BclI | TGATCA | 1 cut(s) | 982 |
| BcnI | CCSGG | 2 cut(s) | 292, 479 |
| BcoDI | GTCTC | 3 cut(s) | 519, 529, 791 |
| BfmI | CTRYAG | 1 cut(s) | 1074 |
| BfoI | RGCGCY | 1 cut(s) | 291 |
| BfuAI | ACCTGC | 1 cut(s) | 162 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 36, 285 |
| BglII | AGATCT | 2 cut(s) | 230, 249 |
| BisI | GCNGC | 5 cut(s) | 28, 37, 122, 169, 906 |
| BlpI | GCTNAGC | 1 cut(s) | 650 |
| BlsI | GCNGC | 5 cut(s) | 29, 38, 123, 170, 907 |
| BmcAI | AGTACT | 1 cut(s) | 1304 |
| Bme1390I | CCNGG | 6 cut(s) | 23, 210, 292, 479, 729, 1277 |
| Bme18I | GGWCC | 1 cut(s) | 236 |
| BmeT110I | CYCGRG | 1 cut(s) | 203 |
| BmgT120I | GGNCC | 1 cut(s) | 236 |
| BmiI | GGNNCC | 6 cut(s) | 52, 97, 214, 238, 289, 725 |
| BmrFI | CCNGG | 6 cut(s) | 23, 210, 292, 479, 729, 1277 |
| BmrI | ACTGGG | 1 cut(s) | 310 |
| BmsI | GCATC | 3 cut(s) | 96, 480, 1270 |
| BmuI | ACTGGG | 1 cut(s) | 310 |
| BpiI | GAAGAC | 1 cut(s) | 265 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 177, 209 |
| Bpu10I | CCTNAGC | 2 cut(s) | 306, 1290 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 650 |
| Bpu14I | TTCGAA | 1 cut(s) | 458 |
| BpuEI | CTTGAG | 2 cut(s) | 152, 629 |
| BpuMI | CCSGG | 2 cut(s) | 292, 479 |
| BsaHI | GRCGYC | 1 cut(s) | 288 |
| BsaI | GGTCTC | 1 cut(s) | 529 |
| BsaJI | CCNNGG | 6 cut(s) | 22, 216, 727, 728, 919, 969 |
| BsaWI | WCCGGW | 1 cut(s) | 98 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1286, 1316 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 22, 60, 291, 665, 920, 951, 1076 |
| Bse1I | ACTGG | 6 cut(s) | 31, 162, 280, 305, 433, 1201 |
| Bse21I | CCTNAGG | 1 cut(s) | 749 |
| Bse3DI | GCAATG | 1 cut(s) | 1000 |
| BseBI | CCWGG | 4 cut(s) | 23, 210, 729, 1277 |
| BseDI | CCNNGG | 6 cut(s) | 22, 216, 727, 728, 919, 969 |
| BseGI | GGATG | 6 cut(s) | 18, 492, 985, 1216, 1285, 1303 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 22, 60, 291, 665, 920, 951, 1076 |
| BseMI | GCAATG | 1 cut(s) | 1000 |
| BseMII | CTCAG | 3 cut(s) | 321, 664, 1281 |
| BseNI | ACTGG | 6 cut(s) | 31, 162, 280, 305, 433, 1201 |
| BseRI | GAGGAG | 3 cut(s) | 435, 485, 1307 |
| BseSI | GKGCMC | 1 cut(s) | 570 |
| BseXI | GCAGC | 1 cut(s) | 917 |
| BseYI | CCCAGC | 1 cut(s) | 870 |
| BshFI | GGCC | 6 cut(s) | 30, 161, 246, 259, 279, 371 |
| BshNI | GGYRCC | 3 cut(s) | 95, 212, 287 |
| BsiHKAI | GWGCWC | 4 cut(s) | 84, 195, 570, 968 |
| BsiHKCI | CYCGRG | 1 cut(s) | 203 |
| BsiSI | CCGG | 4 cut(s) | 99, 285, 291, 479 |
| BslFI | GGGAC | 3 cut(s) | 222, 439, 457 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 22, 60, 291, 665, 920, 951, 1076 |
| BsmAI | GTCTC | 3 cut(s) | 519, 529, 791 |
| BsmBI | CGTCTC | 2 cut(s) | 519, 791 |
| BsmFI | GGGAC | 3 cut(s) | 222, 439, 457 |
| BsmI | GAATGC | 1 cut(s) | 589 |
| BsnI | GGCC | 6 cut(s) | 30, 161, 246, 259, 279, 371 |
| Bso31I | GGTCTC | 1 cut(s) | 529 |
| BsoBI | CYCGRG | 1 cut(s) | 203 |
| Bsp119I | TTCGAA | 1 cut(s) | 458 |
| Bsp1286I | GDGCHC | 5 cut(s) | 84, 195, 546, 570, 968 |
| Bsp1407I | TGTACA | 1 cut(s) | 840 |
| Bsp143I | GATC | 9 cut(s) | 45, 138, 199, 230, 249, 336, 431, 982, 1249 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 650 |
| Bsp19I | CCATGG | 2 cut(s) | 216, 919 |
| BspACI | CCGC | 5 cut(s) | 27, 36, 122, 168, 637 |
| BspANI | GGCC | 6 cut(s) | 30, 161, 246, 259, 279, 371 |
| BspCNI | CTCAG | 3 cut(s) | 322, 663, 1282 |
| BspLI | GGNNCC | 6 cut(s) | 52, 97, 214, 238, 289, 725 |
| BspMI | ACCTGC | 1 cut(s) | 162 |
| BspPI | GGATC | 3 cut(s) | 146, 207, 439 |
| BspT104I | TTCGAA | 1 cut(s) | 458 |
| BspT107I | GGYRCC | 3 cut(s) | 95, 212, 287 |
| BspTNI | GGTCTC | 1 cut(s) | 529 |
| BsrDI | GCAATG | 1 cut(s) | 1000 |
| BsrGI | TGTACA | 1 cut(s) | 840 |
| BsrI | ACTGG | 6 cut(s) | 31, 162, 280, 305, 433, 1201 |
| BssECI | CCNNGG | 6 cut(s) | 22, 216, 727, 728, 919, 969 |
| BssMI | GATC | 9 cut(s) | 45, 138, 199, 230, 249, 336, 431, 982, 1249 |
| BssNI | GRCGYC | 1 cut(s) | 288 |
| BssSI | CACGAG | 1 cut(s) | 825 |
| BssT1I | CCWWGG | 3 cut(s) | 216, 919, 969 |
| Bst2BI | CACGAG | 1 cut(s) | 825 |
| Bst2UI | CCWGG | 4 cut(s) | 23, 210, 729, 1277 |
| Bst4CI | ACNGT | 3 cut(s) | 535, 856, 1057 |
| Bst6I | CTCTTC | 3 cut(s) | 1018, 1305, 1311 |
| BstACI | GRCGYC | 1 cut(s) | 288 |
| BstAUI | TGTACA | 1 cut(s) | 840 |
| BstBI | TTCGAA | 1 cut(s) | 458 |
| BstDEI | CTNAG | 6 cut(s) | 306, 330, 650, 749, 888, 1290 |
| BstDSI | CCRYGG | 2 cut(s) | 216, 919 |
| BstF5I | GGATG | 6 cut(s) | 18, 492, 985, 1216, 1285, 1303 |
| BstH2I | RGCGCY | 1 cut(s) | 291 |
| BstHHI | GCGC | 3 cut(s) | 77, 290, 905 |
| BstKTI | GATC | 9 cut(s) | 48, 141, 202, 233, 252, 339, 434, 985, 1252 |
| BstMAI | GTCTC | 3 cut(s) | 519, 529, 791 |
| BstMBI | GATC | 9 cut(s) | 45, 138, 199, 230, 249, 336, 431, 982, 1249 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 33, 36, 93, 127, 285, 660 |
| BstNI | CCWGG | 4 cut(s) | 23, 210, 729, 1277 |
| BstSCI | CCNGG | 6 cut(s) | 21, 208, 290, 477, 727, 1275 |
| BstSFI | CTRYAG | 1 cut(s) | 1074 |
| BstSLI | GKGCMC | 1 cut(s) | 570 |
| BstV1I | GCAGC | 1 cut(s) | 917 |
| BstV2I | GAAGAC | 1 cut(s) | 265 |
| BstX2I | RGATCY | 4 cut(s) | 199, 230, 249, 431 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 976 |
| BstYI | RGATCY | 4 cut(s) | 199, 230, 249, 431 |
| Bsu36I | CCTNAGG | 1 cut(s) | 749 |
| BsuRI | GGCC | 6 cut(s) | 30, 161, 246, 259, 279, 371 |
| BtgI | CCRYGG | 2 cut(s) | 216, 919 |
| BtsCI | GGATG | 6 cut(s) | 18, 492, 985, 1216, 1285, 1303 |
| BtsI | GCAGTG | 2 cut(s) | 933, 1155 |
| BtsIMutI | CAGTG | 5 cut(s) | 38, 933, 1053, 1155, 1194 |
| BveI | ACCTGC | 1 cut(s) | 162 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1232 |
| CfoI | GCGC | 3 cut(s) | 77, 290, 905 |
| Cfr13I | GGNCC | 1 cut(s) | 236 |
| CseI | GACGC | 1 cut(s) | 214 |
| Csp6I | GTAC | 6 cut(s) | 106, 475, 841, 1192, 1273, 1303 |
| CviQI | GTAC | 6 cut(s) | 106, 475, 841, 1192, 1273, 1303 |
| DdeI | CTNAG | 6 cut(s) | 306, 330, 650, 749, 888, 1290 |
| DinI | GGCGCC | 1 cut(s) | 289 |
| DpnI | GATC | 9 cut(s) | 47, 140, 201, 232, 251, 338, 433, 984, 1251 |
| DpnII | GATC | 9 cut(s) | 45, 138, 199, 230, 249, 336, 431, 982, 1249 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 269 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 269 |
| EaeI | YGGCCR | 3 cut(s) | 28, 244, 277 |
| Eam1104I | CTCTTC | 3 cut(s) | 1018, 1305, 1311 |
| EarI | CTCTTC | 3 cut(s) | 1018, 1305, 1311 |
| Eco130I | CCWWGG | 3 cut(s) | 216, 919, 969 |
| Eco147I | AGGCCT | 1 cut(s) | 259 |
| Eco24I | GRGCYC | 1 cut(s) | 546 |
| Eco31I | GGTCTC | 1 cut(s) | 529 |
| Eco32I | GATATC | 1 cut(s) | 1066 |
| Eco47I | GGWCC | 1 cut(s) | 236 |
| Eco57I | CTGAAG | 2 cut(s) | 110, 238 |
| Eco81I | CCTNAGG | 1 cut(s) | 749 |
| Eco88I | CYCGRG | 1 cut(s) | 203 |
| EcoRII | CCWGG | 4 cut(s) | 21, 208, 727, 1275 |
| EcoRV | GATATC | 1 cut(s) | 1066 |
| EcoT14I | CCWWGG | 3 cut(s) | 216, 919, 969 |
| EcoT38I | GRGCYC | 1 cut(s) | 546 |
| EgeI | GGCGCC | 1 cut(s) | 289 |
| EheI | GGCGCC | 1 cut(s) | 289 |
| ErhI | CCWWGG | 3 cut(s) | 216, 919, 969 |
| Esp3I | CGTCTC | 2 cut(s) | 519, 791 |
| FaqI | GGGAC | 3 cut(s) | 222, 439, 457 |
| FbaI | TGATCA | 1 cut(s) | 982 |
| FblI | GTMKAC | 1 cut(s) | 147 |
| Fnu4HI | GCNGC | 5 cut(s) | 28, 37, 122, 169, 906 |
| FokI | GGATG | 6 cut(s) | 5, 499, 992, 1223, 1292, 1310 |
| FriOI | GRGCYC | 1 cut(s) | 546 |
| Fsp4HI | GCNGC | 5 cut(s) | 28, 37, 122, 169, 906 |
| FspI | TGCGCA | 1 cut(s) | 904 |
| GlaI | GCGC | 3 cut(s) | 76, 289, 904 |
| GluI | GCNGC | 5 cut(s) | 28, 37, 122, 169, 906 |
| GsaI | CCCAGC | 1 cut(s) | 874 |
| HaeII | RGCGCY | 1 cut(s) | 291 |
| HaeIII | GGCC | 6 cut(s) | 30, 161, 246, 259, 279, 371 |
| HapII | CCGG | 4 cut(s) | 99, 285, 291, 479 |
| HgaI | GACGC | 1 cut(s) | 214 |
| HhaI | GCGC | 3 cut(s) | 77, 290, 905 |
| Hin1I | GRCGYC | 1 cut(s) | 288 |
| Hin6I | GCGC | 3 cut(s) | 75, 288, 903 |
| HinP1I | GCGC | 3 cut(s) | 75, 288, 903 |
| HincII | GTYRAC | 1 cut(s) | 852 |
| HindII | GTYRAC | 1 cut(s) | 852 |
| HindIII | AAGCTT | 1 cut(s) | 661 |
| HinfI | GANTC | 6 cut(s) | 10, 116, 341, 445, 884, 1033 |
| HpaII | CCGG | 4 cut(s) | 99, 285, 291, 479 |
| HphI | GGTGA | 2 cut(s) | 859, 1211 |
| Hpy166II | GTNNAC | 8 cut(s) | 148, 568, 833, 852, 1053, 1155, 1194, 1224 |
| Hpy188I | TCNGA | 6 cut(s) | 50, 230, 529, 745, 1002, 1261 |
| Hpy188III | TCNNGA | 6 cut(s) | 203, 350, 605, 1124, 1168, 1334 |
| Hpy8I | GTNNAC | 8 cut(s) | 148, 568, 833, 852, 1053, 1155, 1194, 1224 |
| Hpy99I | CGWCG | 2 cut(s) | 95, 1140 |
| HpyAV | CCTTC | 7 cut(s) | 96, 104, 317, 1108, 1168, 1196, 1322 |
| HpyCH4III | ACNGT | 3 cut(s) | 535, 856, 1057 |
| HpyCH4IV | ACGT | 1 cut(s) | 497 |
| HpyCH4V | TGCA | 5 cut(s) | 568, 587, 597, 1186, 1283 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 33, 36, 93, 127, 285, 660 |
| HpyF3I | CTNAG | 6 cut(s) | 306, 330, 650, 749, 888, 1290 |
| HpySE526I | ACGT | 1 cut(s) | 497 |
| Hsp92I | GRCGYC | 1 cut(s) | 288 |
| HspAI | GCGC | 3 cut(s) | 75, 288, 903 |
| KasI | GGCGCC | 1 cut(s) | 287 |
| Ksp22I | TGATCA | 1 cut(s) | 982 |
| Kzo9I | GATC | 9 cut(s) | 45, 138, 199, 230, 249, 336, 431, 982, 1249 |
| LmnI | GCTCC | 2 cut(s) | 50, 817 |
| Lsp1109I | GCAGC | 1 cut(s) | 917 |
| LweI | GCATC | 3 cut(s) | 96, 480, 1270 |
| MaeII | ACGT | 1 cut(s) | 497 |
| MaeIII | GTNAC | 2 cut(s) | 380, 706 |
| MalI | GATC | 9 cut(s) | 47, 140, 201, 232, 251, 338, 433, 984, 1251 |
| MboI | GATC | 9 cut(s) | 45, 138, 199, 230, 249, 336, 431, 982, 1249 |
| MboII | GAAGA | 8 cut(s) | 244, 265, 1005, 1096, 1322, 1328, 1334, 1337 |
| MflI | RGATCY | 4 cut(s) | 199, 230, 249, 431 |
| MhlI | GDGCHC | 5 cut(s) | 84, 195, 546, 570, 968 |
| MluCI | AATT | 1 cut(s) | 376 |
| Mly113I | GGCGCC | 1 cut(s) | 288 |
| MlyI | GAGTC | 3 cut(s) | 110, 335, 439 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1178 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1160 |
| MspA1I | CMGCKG | 2 cut(s) | 908, 1289 |
| MspI | CCGG | 4 cut(s) | 99, 285, 291, 479 |
| MspR9I | CCNGG | 6 cut(s) | 23, 210, 292, 479, 729, 1277 |
| Mva1269I | GAATGC | 1 cut(s) | 589 |
| MvaI | CCWGG | 4 cut(s) | 23, 210, 729, 1277 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 33, 36, 93, 127, 285, 660 |
| NarI | GGCGCC | 1 cut(s) | 288 |
| NciI | CCSGG | 2 cut(s) | 292, 479 |
| NcoI | CCATGG | 2 cut(s) | 216, 919 |
| NdeII | GATC | 9 cut(s) | 45, 138, 199, 230, 249, 336, 431, 982, 1249 |
| NlaIV | GGNNCC | 6 cut(s) | 52, 97, 214, 238, 289, 725 |
| NmeAIII | GCCGAG | 2 cut(s) | 103, 397 |
| NmuCI | GTSAC | 2 cut(s) | 380, 706 |
| NsbI | TGCGCA | 1 cut(s) | 904 |
| NspV | TTCGAA | 1 cut(s) | 458 |
| PaeR7I | CTCGAG | 1 cut(s) | 203 |
| PasI | CCCWGGG | 1 cut(s) | 728 |
| PceI | AGGCCT | 1 cut(s) | 259 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 75, 922 |
| PctI | GAATGC | 1 cut(s) | 589 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1178 |
| PfeI | GAWTC | 3 cut(s) | 10, 884, 1033 |
| PflFI | GACNNNGTC | 1 cut(s) | 224 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 60, 665 |
| PkrI | GCNGC | 5 cut(s) | 29, 38, 123, 170, 907 |
| PleI | GAGTC | 3 cut(s) | 110, 335, 439 |
| PluTI | GGCGCC | 1 cut(s) | 291 |
| PpsI | GAGTC | 3 cut(s) | 110, 335, 439 |
| Psp6I | CCWGG | 4 cut(s) | 21, 208, 727, 1275 |
| PspFI | CCCAGC | 1 cut(s) | 870 |
| PspGI | CCWGG | 4 cut(s) | 21, 208, 727, 1275 |
| PspN4I | GGNNCC | 6 cut(s) | 52, 97, 214, 238, 289, 725 |
| PspPI | GGNCC | 1 cut(s) | 236 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1001, 1033 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1232 |
| PsuI | RGATCY | 4 cut(s) | 199, 230, 249, 431 |
| PsyI | GACNNNGTC | 1 cut(s) | 224 |
| PvuII | CAGCTG | 2 cut(s) | 908, 1289 |
| RsaI | GTAC | 6 cut(s) | 107, 476, 842, 1193, 1274, 1304 |
| RsaNI | GTAC | 6 cut(s) | 106, 475, 841, 1192, 1273, 1303 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1160 |
| SatI | GCNGC | 5 cut(s) | 28, 37, 122, 169, 906 |
| Sau3AI | GATC | 9 cut(s) | 45, 138, 199, 230, 249, 336, 431, 982, 1249 |
| Sau96I | GGNCC | 1 cut(s) | 236 |
| ScaI | AGTACT | 1 cut(s) | 1304 |
| SchI | GAGTC | 3 cut(s) | 110, 335, 439 |
| ScrFI | CCNGG | 6 cut(s) | 23, 210, 292, 479, 729, 1277 |
| SduI | GDGCHC | 5 cut(s) | 84, 195, 546, 570, 968 |
| SfaNI | GCATC | 3 cut(s) | 96, 480, 1270 |
| SfcI | CTRYAG | 1 cut(s) | 1074 |
| SfoI | GGCGCC | 1 cut(s) | 289 |
| Sfr274I | CTCGAG | 1 cut(s) | 203 |
| SfuI | TTCGAA | 1 cut(s) | 458 |
| SinI | GGWCC | 1 cut(s) | 236 |
| SlaI | CTCGAG | 1 cut(s) | 203 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1160 |
| SmlI | CTYRAG | 3 cut(s) | 131, 203, 644 |
| SmoI | CTYRAG | 3 cut(s) | 131, 203, 644 |
| Sse9I | AATT | 1 cut(s) | 376 |
| SseBI | AGGCCT | 1 cut(s) | 259 |
| SsiI | CCGC | 5 cut(s) | 27, 36, 122, 168, 637 |
| SspDI | GGCGCC | 1 cut(s) | 287 |
| StuI | AGGCCT | 1 cut(s) | 259 |
| StyD4I | CCNGG | 6 cut(s) | 21, 208, 290, 477, 727, 1275 |
| StyI | CCWWGG | 3 cut(s) | 216, 919, 969 |
| TaaI | ACNGT | 3 cut(s) | 535, 856, 1057 |
| TaiI | ACGT | 1 cut(s) | 500 |
| TaqI | TCGA | 5 cut(s) | 90, 204, 339, 351, 458 |
| TasI | AATT | 1 cut(s) | 376 |
| TatI | WGTACW | 2 cut(s) | 840, 1302 |
| TauI | GCSGC | 4 cut(s) | 30, 39, 124, 171 |
| TfiI | GAWTC | 3 cut(s) | 10, 884, 1033 |
| TscAI | CASTG | 5 cut(s) | 38, 940, 1060, 1162, 1201 |
| TseFI | GTSAC | 2 cut(s) | 380, 706 |
| TseI | GCWGC | 1 cut(s) | 905 |
| Tsp45I | GTSAC | 2 cut(s) | 380, 706 |
| TspDTI | ATGAA | 5 cut(s) | 787, 1005, 1090, 1259, 1323 |
| TspGWI | ACGGA | 1 cut(s) | 1124 |
| TspRI | CASTG | 5 cut(s) | 38, 940, 1060, 1162, 1201 |
| Tth111I | GACNNNGTC | 1 cut(s) | 224 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 60, 665 |
| VneI | GTGCAC | 1 cut(s) | 566 |
| VpaK11BI | GGWCC | 1 cut(s) | 236 |
| XhoI | CTCGAG | 1 cut(s) | 203 |
| XmiI | GTMKAC | 1 cut(s) | 147 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1178 |
| ZrmI | AGTACT | 1 cut(s) | 1304 |