MD04G1143700.v1.1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012720
Physical Location & Seq
Reverse (-)
23284338 .. 23288643
4306 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1143700.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGCGTGAGATCCTTCACATCCAGGGAGGCCAGTGCGGCAACCAGATTGGCGCCAAGTTCTGGGAGGTGGTCTGCGCCGAGCACGGCATCGACGGCACCGGTAGGTACGAGGGAGACTCGGAGCTCCAGCTTGAGAGGATCAATGTCTACTACAACGAAGCCAGTTGCGGGAGGTACGTGCCGAGGGCTGTGCTCATGGACCTCGAGCCTGGGACCATGGACAGCGTCCGATCTGGACCCTACGGTCAGATTTTCAGGCCTGACAACTTCGTTTTTGGCCAGTCTGGTGCCGGGAACAACTGGGCTAAGGGTCACTATACCGAAGGCGCTGAGTTGATCGATTCGGTTCTTGATGTGGTTCGCAAGGAGGCCGAGAATTGTGATTGCCTGCAAGGGTTTCAGGTTTGTCATTCTCTGGGAGGAGGTACTGGGTCTGGCATGGGTACTCTGTTGATTTCAAAGATCAGAGAGGAATACCCAGATCGGATGATGCTCACTTTCTCGGTTTTTCCCTCCCCAAAGGTGTCGGACACTGTAGTTGAGCCTTACAATGCGACCTTGTCTGTGCACCAGCTTGTTGAAAATGCAGATGAGTGTATGGTTCTTGATAATGAAGCTCTGTATGACATTTGTTTCCGCACACTCAAGCTCAGCACCCCGAGCTTTGGAGATTTGAACCATTTGATTTCTGCAACTATGTCTGGTGTCACATGCTGTTTGAGGTTCCCTGGTCAGTTGAACTCTGACCTCCGAAAGCTTGCTGTCAATCTCATCCCCTTCCCTCGTCTCCACTTCTTCATGGTGGGTTTTGCTCCTTTGACCTCACGCGGTTCACAGCAGTACCGAGCTCTCACTGTGCCTGAGCTCACACAGCAAATGTGGGATGCGAAGAACATGATGTGTGCAGCTGACCCTCGCCATGGTCGGTATTTGACTGCCTCAGCCATGTTCAGGGGTAAAATGAGCACAAAGGAAGTTGATGAGCAGATGATCAATGTCCAGAACAAGAACTCTTCTTACTTTGTGGAATGGATTCCCAACAATGTCAAGTCTACTGTATGTGACATCCCTCCCACCGGCTTGAAAATGGCATCCACCTTCATTGGAAACTCAACCTCAATTCAGGAGATGTTCCGCCGTGTGAGCGAGCAGTTCACAGCTATGTTCAGGAGGAAGGCTTTCTTGCATTGGTACACTGGTGAAGGCATGGACGAGATGGAGTTCACAGAAGCTGAAAGCAACATGAATGATCTGGTTTCGGAGTATCAGCAGTACCAGGATGCCACAGCCGAGGAGGATGAGTACTATGAGGACGAAGAGGAGGAAGCTCAGGAGGAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

448

Amino Acids

50.39

Weight (kDa)

4.71

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 211 1.2e-64 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 261 - 382 1.7e-40 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62690 AT5G62700
fragaria_vesca FvH4_6g12290
malus_domestica MD04G1143700.v1.1 MD12G1158100.v1.1
prunus_persica Prupe.6G268300_v2.0.a1
pyrus_communis pycom04g13060 pycom05g25220 pycom06g07900 pycom07g24790 pycom11g07310 pycom12g15020
rosa_chinensis RchiOBHm_Chr3g0464121
rosa_laevigata RLG00000024721
rosa_multiflora Rmu_sc0010401.1_g000003
rosa_roxburghii Rroxscaffold_6G00415980
rosa_rugosa Rorug03G0070700
rosa_wichuraiana Rw3G010800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 243
AccB1I GGYRCC 3 cut(s) 50, 95, 287
AccB7I CCANNNNNTGG 1 cut(s) 60
AccI GTMKAC 2 cut(s) 147, 1052
AccII CGCG 1 cut(s) 828
AciI CCGC 5 cut(s) 36, 168, 637, 828, 1135
AclWI GGATC 2 cut(s) 4, 146
AcoI YGGCCR 1 cut(s) 277
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 8 cut(s) 107, 176, 427, 445, 842, 1193, 1274, 1304
AfiI CCNNNNNNNGG 6 cut(s) 60, 168, 665, 920, 951, 1076
AgeI ACCGGT 1 cut(s) 98
AgsI TTSAA 5 cut(s) 459, 581, 676, 739, 1084
AjnI CCWGG 4 cut(s) 21, 208, 727, 1275
Alw21I GWGCWC 7 cut(s) 84, 126, 195, 570, 850, 867, 968
Alw26I GTCTC 2 cut(s) 108, 791
Alw44I GTGCAC 1 cut(s) 566
AlwI GGATC 2 cut(s) 4, 146
AlwNI CAGNNNCTG 1 cut(s) 1232
Ama87I CYCGRG 2 cut(s) 203, 658
AoxI GGCC 4 cut(s) 28, 257, 277, 369
ApaLI GTGCAC 1 cut(s) 566
ApeKI GCWGC 1 cut(s) 905
AsiGI ACCGGT 1 cut(s) 98
Asp700I GAANNNNTTC 2 cut(s) 1032, 1178
AspLEI GCGC 3 cut(s) 53, 77, 329
AspS9I GGNCC 3 cut(s) 199, 213, 236
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 1211
AvaI CYCGRG 2 cut(s) 203, 658
AvaII GGWCC 3 cut(s) 199, 213, 236
BaeGI GKGCMC 1 cut(s) 570
BaeI ACNNNNGTAYC 4 cut(s) 824, 857, 1183, 1216
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 3 cut(s) 50, 95, 287
BanII GRGCYC 3 cut(s) 126, 850, 867
Bbv12I GWGCWC 7 cut(s) 84, 126, 195, 570, 850, 867, 968
BbvCI CCTCAGC 1 cut(s) 940
BbvI GCAGC 1 cut(s) 917
BccI CCATC 1 cut(s) 1210
BceAI ACGGC 3 cut(s) 100, 109, 1122
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 4 cut(s) 23, 210, 729, 1277
BclI TGATCA 1 cut(s) 990
BcnI CCSGG 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 108, 791
BfmI CTRYAG 1 cut(s) 534
BfoI RGCGCY 2 cut(s) 54, 330
BglI GCCNNNNNGGC 1 cut(s) 36
BisI GCNGC 2 cut(s) 37, 906
BlpI GCTNAGC 1 cut(s) 650
BlsI GCNGC 2 cut(s) 38, 907
BmcAI AGTACT 1 cut(s) 1304
Bme1390I CCNGG 5 cut(s) 23, 210, 292, 729, 1277
Bme18I GGWCC 3 cut(s) 199, 213, 236
BmeT110I CYCGRG 2 cut(s) 203, 658
BmgT120I GGNCC 3 cut(s) 199, 213, 236
BmiI GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
BmrFI CCNGG 5 cut(s) 23, 210, 292, 729, 1277
BmrI ACTGGG 2 cut(s) 310, 438
BmsI GCATC 5 cut(s) 96, 480, 874, 1100, 1270
BmuI ACTGGG 2 cut(s) 310, 438
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 4 cut(s) 306, 861, 940, 1329
Bpu1102I GCTNAGC 1 cut(s) 650
BpuEI CTTGAG 2 cut(s) 152, 629
BpuMI CCSGG 1 cut(s) 292
Bsa29I ATCGAT 1 cut(s) 339
BsaAI YACGTR 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 7 cut(s) 22, 182, 209, 216, 727, 919, 1290
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 3 cut(s) 1286, 1316, 1325
Bsc4I CCNNNNNNNGG 6 cut(s) 60, 168, 665, 920, 951, 1076
Bse118I RCCGGY 2 cut(s) 98, 1076
Bse1I ACTGG 6 cut(s) 31, 162, 280, 305, 433, 1201
BseBI CCWGG 4 cut(s) 23, 210, 729, 1277
BseCI ATCGAT 1 cut(s) 339
BseDI CCNNGG 7 cut(s) 22, 182, 209, 216, 727, 919, 1290
BseGI GGATG 8 cut(s) 18, 492, 771, 889, 1065, 1091, 1285, 1303
BseLI CCNNNNNNNGG 6 cut(s) 60, 168, 665, 920, 951, 1076
BseMII CTCAG 5 cut(s) 321, 664, 852, 954, 1343
BseNI ACTGG 6 cut(s) 31, 162, 280, 305, 433, 1201
BseRI GAGGAG 3 cut(s) 435, 1307, 1334
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 1 cut(s) 917
BsgI GTGCAG 1 cut(s) 924
Bsh1236I CGCG 1 cut(s) 828
BshFI GGCC 4 cut(s) 30, 259, 279, 371
BshNI GGYRCC 3 cut(s) 50, 95, 287
BshTI ACCGGT 1 cut(s) 98
BshVI ATCGAT 1 cut(s) 339
BsiHKAI GWGCWC 7 cut(s) 84, 126, 195, 570, 850, 867, 968
BsiHKCI CYCGRG 2 cut(s) 203, 658
BsiSI CCGG 3 cut(s) 99, 291, 1077
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 6 cut(s) 60, 168, 665, 920, 951, 1076
BsmAI GTCTC 2 cut(s) 108, 791
BsmBI CGTCTC 1 cut(s) 791
BsmFI GGGAC 1 cut(s) 226
BsnI GGCC 4 cut(s) 30, 259, 279, 371
BsoBI CYCGRG 2 cut(s) 203, 658
Bsp1286I GDGCHC 7 cut(s) 84, 126, 195, 570, 850, 867, 968
Bsp143I GATC 8 cut(s) 9, 138, 230, 336, 462, 481, 990, 1249
Bsp1720I GCTNAGC 1 cut(s) 650
Bsp19I CCATGG 2 cut(s) 216, 919
BspACI CCGC 5 cut(s) 36, 168, 637, 828, 1135
BspANI GGCC 4 cut(s) 30, 259, 279, 371
BspCNI CTCAG 5 cut(s) 322, 663, 853, 953, 1342
BspDI ATCGAT 1 cut(s) 339
BspFNI CGCG 1 cut(s) 828
BspLI GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
BspPI GGATC 2 cut(s) 4, 146
BspT107I GGYRCC 3 cut(s) 50, 95, 287
BsrFI RCCGGY 2 cut(s) 98, 1076
BsrI ACTGG 6 cut(s) 31, 162, 280, 305, 433, 1201
BssAI RCCGGY 2 cut(s) 98, 1076
BssECI CCNNGG 7 cut(s) 22, 182, 209, 216, 727, 919, 1290
BssMI GATC 8 cut(s) 9, 138, 230, 336, 462, 481, 990, 1249
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 216, 919
Bst2UI CCWGG 4 cut(s) 23, 210, 729, 1277
Bst4CI ACNGT 4 cut(s) 245, 535, 856, 1057
Bst6I CTCTTC 2 cut(s) 1018, 1311
BstACI GRCGYC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 178
BstC8I GCNNGC 3 cut(s) 389, 759, 1148
BstDEI CTNAG 6 cut(s) 306, 330, 650, 861, 940, 1329
BstDSI CCRYGG 2 cut(s) 216, 919
BstF5I GGATG 8 cut(s) 18, 492, 771, 889, 1065, 1091, 1285, 1303
BstFNI CGCG 1 cut(s) 828
BstH2I RGCGCY 2 cut(s) 54, 330
BstHHI GCGC 3 cut(s) 53, 77, 329
BstKTI GATC 8 cut(s) 12, 141, 233, 339, 465, 484, 993, 1252
BstMAI GTCTC 2 cut(s) 108, 791
BstMBI GATC 8 cut(s) 9, 138, 230, 336, 462, 481, 990, 1249
BstMWI GCNNNNNNNGC 5 cut(s) 36, 93, 660, 871, 1143
BstNI CCWGG 4 cut(s) 23, 210, 729, 1277
BstNSI RCATGY 1 cut(s) 714
BstSCI CCNGG 5 cut(s) 21, 208, 290, 727, 1275
BstSFI CTRYAG 1 cut(s) 534
BstSLI GKGCMC 1 cut(s) 570
BstUI CGCG 1 cut(s) 828
BstV1I GCAGC 1 cut(s) 917
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
Bsu15I ATCGAT 1 cut(s) 339
BsuRI GGCC 4 cut(s) 30, 259, 279, 371
BsuTUI ATCGAT 1 cut(s) 339
BtgI CCRYGG 2 cut(s) 216, 919
BtsCI GGATG 8 cut(s) 18, 492, 771, 889, 1065, 1091, 1285, 1303
BtsIMutI CAGTG 4 cut(s) 38, 531, 852, 1194
Cac8I GCNNGC 3 cut(s) 389, 759, 1148
CaiI CAGNNNCTG 1 cut(s) 1232
CfoI GCGC 3 cut(s) 53, 77, 329
Cfr10I RCCGGY 2 cut(s) 98, 1076
Cfr13I GGNCC 3 cut(s) 199, 213, 236
ClaI ATCGAT 1 cut(s) 339
CseI GACGC 1 cut(s) 214
Csp6I GTAC 8 cut(s) 106, 175, 426, 444, 841, 1192, 1273, 1303
CspAI ACCGGT 1 cut(s) 98
CspCI CAANNNNNGTGG 2 cut(s) 1084, 1119
CviQI GTAC 8 cut(s) 106, 175, 426, 444, 841, 1192, 1273, 1303
DdeI CTNAG 6 cut(s) 306, 330, 650, 861, 940, 1329
DinI GGCGCC 1 cut(s) 52
DpnI GATC 8 cut(s) 11, 140, 232, 338, 464, 483, 992, 1251
DpnII GATC 8 cut(s) 9, 138, 230, 336, 462, 481, 990, 1249
DrdI GACNNNNNNGTC 1 cut(s) 243
DseDI GACNNNNNNGTC 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 1018, 1311
EarI CTCTTC 2 cut(s) 1018, 1311
EciI GGCGGA 1 cut(s) 1124
Ecl136II GAGCTC 3 cut(s) 124, 848, 865
Eco130I CCWWGG 2 cut(s) 216, 919
Eco147I AGGCCT 1 cut(s) 259
Eco24I GRGCYC 3 cut(s) 126, 850, 867
Eco47I GGWCC 3 cut(s) 199, 213, 236
Eco53kI GAGCTC 3 cut(s) 124, 848, 865
Eco88I CYCGRG 2 cut(s) 203, 658
EcoICRI GAGCTC 3 cut(s) 124, 848, 865
EcoRII CCWGG 4 cut(s) 21, 208, 727, 1275
EcoT14I CCWWGG 2 cut(s) 216, 919
EcoT38I GRGCYC 3 cut(s) 126, 850, 867
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 216, 919
Esp3I CGTCTC 1 cut(s) 791
FalI AAGNNNNNCTT 2 cut(s) 1166, 1198
FaqI GGGAC 1 cut(s) 226
FauI CCCGC 1 cut(s) 161
FbaI TGATCA 1 cut(s) 990
FblI GTMKAC 2 cut(s) 147, 1052
Fnu4HI GCNGC 2 cut(s) 37, 906
FokI GGATG 8 cut(s) 5, 499, 758, 896, 1052, 1078, 1292, 1310
FriOI GRGCYC 3 cut(s) 126, 850, 867
Fsp4HI GCNGC 2 cut(s) 37, 906
GlaI GCGC 3 cut(s) 52, 76, 328
GluI GCNGC 2 cut(s) 37, 906
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 2 cut(s) 54, 330
HaeIII GGCC 4 cut(s) 30, 259, 279, 371
HapII CCGG 3 cut(s) 99, 291, 1077
HgaI GACGC 1 cut(s) 214
HhaI GCGC 3 cut(s) 53, 77, 329
Hin1I GRCGYC 1 cut(s) 51
Hin6I GCGC 3 cut(s) 51, 75, 327
HinP1I GCGC 3 cut(s) 51, 75, 327
HindIII AAGCTT 1 cut(s) 755
HinfI GANTC 3 cut(s) 116, 341, 1033
HpaII CCGG 3 cut(s) 99, 291, 1077
HphI GGTGA 1 cut(s) 1211
Hpy166II GTNNAC 7 cut(s) 148, 568, 833, 1053, 1155, 1194, 1224
Hpy188I TCNGA 9 cut(s) 121, 230, 249, 467, 486, 529, 745, 752, 1261
Hpy188III TCNNGA 7 cut(s) 234, 350, 605, 1000, 1124, 1168, 1331
Hpy8I GTNNAC 7 cut(s) 148, 568, 833, 1053, 1155, 1194, 1224
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 6 cut(s) 23, 317, 787, 1108, 1168, 1196
HpyCH4III ACNGT 4 cut(s) 245, 535, 856, 1057
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 6 cut(s) 391, 568, 587, 692, 905, 1186
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 93, 660, 871, 1143
HpyF3I CTNAG 6 cut(s) 306, 330, 650, 861, 940, 1329
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 51
HspAI GCGC 3 cut(s) 51, 75, 327
KasI GGCGCC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 990
Kzo9I GATC 8 cut(s) 9, 138, 230, 336, 462, 481, 990, 1249
LmnI GCTCC 3 cut(s) 121, 129, 817
Lsp1109I GCAGC 1 cut(s) 917
LweI GCATC 5 cut(s) 96, 480, 874, 1100, 1270
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 3 cut(s) 311, 706, 1061
MalI GATC 8 cut(s) 11, 140, 232, 338, 464, 483, 992, 1251
MboI GATC 8 cut(s) 9, 138, 230, 336, 462, 481, 990, 1249
MboII GAAGA 4 cut(s) 787, 901, 1005, 1328
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 7 cut(s) 84, 126, 195, 570, 850, 867, 968
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 2 cut(s) 376, 1119
MluNI TGGCCA 1 cut(s) 279
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 507
Mox20I TGGCCA 1 cut(s) 279
MroXI GAANNNNTTC 2 cut(s) 1032, 1178
MscI TGGCCA 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 1160
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 3 cut(s) 99, 291, 1077
MspR9I CCNGG 5 cut(s) 23, 210, 292, 729, 1277
MvaI CCWGG 4 cut(s) 23, 210, 729, 1277
MvnI CGCG 1 cut(s) 828
MwoI GCNNNNNNNGC 5 cut(s) 36, 93, 660, 871, 1143
NarI GGCGCC 1 cut(s) 51
NciI CCSGG 1 cut(s) 292
NcoI CCATGG 2 cut(s) 216, 919
NdeII GATC 8 cut(s) 9, 138, 230, 336, 462, 481, 990, 1249
NlaIV GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
NmeAIII GCCGAG 4 cut(s) 103, 207, 397, 1315
NmuCI GTSAC 3 cut(s) 311, 706, 1061
NspI RCATGY 1 cut(s) 714
PaeR7I CTCGAG 1 cut(s) 203
PceI AGGCCT 1 cut(s) 259
PdmI GAANNNNTTC 2 cut(s) 1032, 1178
PfeI GAWTC 2 cut(s) 341, 1033
PflFI GACNNNGTC 2 cut(s) 224, 559
PflMI CCANNNNNTGG 1 cut(s) 60
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 2 cut(s) 38, 907
PleI GAGTC 1 cut(s) 110
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 110
Ppu21I YACGTR 1 cut(s) 178
Psp124BI GAGCTC 3 cut(s) 126, 850, 867
Psp6I CCWGG 4 cut(s) 21, 208, 727, 1275
PspGI CCWGG 4 cut(s) 21, 208, 727, 1275
PspN4I GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
PspPI GGNCC 3 cut(s) 199, 213, 236
PspXI VCTCGAGB 1 cut(s) 203
PsrI GAACNNNNNNTAC 2 cut(s) 1001, 1033
PstNI CAGNNNCTG 1 cut(s) 1232
PsuI RGATCY 1 cut(s) 9
PsyI GACNNNGTC 2 cut(s) 224, 559
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 8 cut(s) 107, 176, 427, 445, 842, 1193, 1274, 1304
RsaNI GTAC 8 cut(s) 106, 175, 426, 444, 841, 1192, 1273, 1303
RseI CAYNNNNRTG 1 cut(s) 1160
SacI GAGCTC 3 cut(s) 126, 850, 867
SatI GCNGC 2 cut(s) 37, 906
Sau3AI GATC 8 cut(s) 9, 138, 230, 336, 462, 481, 990, 1249
Sau96I GGNCC 3 cut(s) 199, 213, 236
ScaI AGTACT 1 cut(s) 1304
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 5 cut(s) 23, 210, 292, 729, 1277
SduI GDGCHC 7 cut(s) 84, 126, 195, 570, 850, 867, 968
SfaNI GCATC 5 cut(s) 96, 480, 874, 1100, 1270
SfcI CTRYAG 1 cut(s) 534
SfoI GGCGCC 1 cut(s) 52
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 3 cut(s) 199, 213, 236
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 1160
SmlI CTYRAG 3 cut(s) 131, 203, 644
SmoI CTYRAG 3 cut(s) 131, 203, 644
Sse9I AATT 2 cut(s) 376, 1119
SseBI AGGCCT 1 cut(s) 259
SsiI CCGC 5 cut(s) 36, 168, 637, 828, 1135
SspDI GGCGCC 1 cut(s) 50
SstI GAGCTC 3 cut(s) 126, 850, 867
StuI AGGCCT 1 cut(s) 259
StyD4I CCNGG 5 cut(s) 21, 208, 290, 727, 1275
StyI CCWWGG 2 cut(s) 216, 919
TaaI ACNGT 4 cut(s) 245, 535, 856, 1057
TaiI ACGT 1 cut(s) 180
TaqI TCGA 3 cut(s) 90, 204, 339
TasI AATT 2 cut(s) 376, 1119
TatI WGTACW 1 cut(s) 1302
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 2 cut(s) 341, 1033
TscAI CASTG 4 cut(s) 38, 538, 859, 1201
TseFI GTSAC 3 cut(s) 311, 706, 1061
TseI GCWGC 1 cut(s) 905
Tsp45I GTSAC 3 cut(s) 311, 706, 1061
TspDTI ATGAA 4 cut(s) 627, 787, 1090, 1259
TspRI CASTG 4 cut(s) 38, 538, 859, 1201
Tth111I GACNNNGTC 2 cut(s) 224, 559
Van91I CCANNNNNTGG 1 cut(s) 60
VneI GTGCAC 1 cut(s) 566
VpaK11BI GGWCC 3 cut(s) 199, 213, 236
XceI RCATGY 1 cut(s) 714
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 2 cut(s) 147, 1052
XmnI GAANNNNTTC 2 cut(s) 1032, 1178
ZrmI AGTACT 1 cut(s) 1304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.