pycom06g07900

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012720
Physical Location & Seq
Reverse (-)
12884538 .. 12886524
1987 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g07900.3

Sequence Viewer

Length: 1065 bp
ATGAGAGAAATCTTGCACATTCAAGCTGGACAATGTGGGAACCAAATTGGAGGGAAGTTTTGGGAGGTGATGTGTGACGAGCATGGGATCGATCCCACCGGAAAATATAACGGGAACTCCCACCTTCAGCTTGAGAGGGTAAATGTCTACTACAATGAAGCAAATGGAGGAAGATATGTGCCTAGGGCAGTGCTGATGGACCTCGAGCCGGGGACGATGGACAGCTTAAGAACTGGTCCTTATGGGCAGATTTTCAGGCCGGACAACTTTGTGTTTGGCCAGAATGGAGCCGGAAACAATTGGGCTAAAGGGCATTACACAGAAGGAGCTGAGTTGATTGATGCTGTTCTTGATGTGGTTAGAAAAGAGGCTGAGAATTGTGATTGTTTACAAGGGTTCCAGATTTGCCATTCCCTAGGAGGTGGGACAGGGTCAGGAATGGGGACCCTACTGATTTCAAAGATCAGGGAAGAGTACCCAGATAGAATGATGATGACTTTCTCAGTCTTCCCCTCACCAAAAGTCTCAGACACAGTGGTCGAACCCTACAATGCAACCCTCTCTGTTCACCAATTGGTTGAAAATGCTGATGAGGTCATGGTCCTTGACAATGAAGCCCTCTATGATATTTGTTTCAGAACTCTCAAGCTCACAAATCCAAGCTTTGGTGATTTGAACCATTTGATTTCCACAACCATGAGTGGAGTCACATGTTGCCTCCGCTTCCCGGGACAGCTCAACTCCGATCTTCGAAAACTAGCCGTGAATCTCATCCCCTTCCCCCGCCTCCATTTCTTCATGGTTGGTTTCGCACCCTTGACCTCCCGCGGCTCCCAACTCTATAGAGCCCTCACAATCCCTGAGCTCACACAACAAATGTGGGACGCCAAGAACATGATGTGCGCCGCAGATCCACGCCATGGCCGATACCTGACAGCATCGGCTGTGTTCCGCGGCAAAATGAGCACAAAAGAAGTGGACGAGCAGATCGATCAACATACAAAACAAGAACTCCTCATTCTTTGTGGAGTGGATTCCAAACAATGTCAAATCAAGTGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

355

Amino Acids

39.49

Weight (kDa)

5.7

Isoelectric Point (pI)

25.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62690 AT5G62700
fragaria_vesca FvH4_6g12290
malus_domestica MD04G1143700.v1.1 MD12G1158100.v1.1
prunus_persica Prupe.6G268300_v2.0.a1
pyrus_communis pycom04g13060 pycom05g25220 pycom06g07900 pycom07g24790 pycom11g07310 pycom12g15020
rosa_chinensis RchiOBHm_Chr3g0464121
rosa_laevigata RLG00000024721
rosa_multiflora Rmu_sc0010401.1_g000003
rosa_roxburghii Rroxscaffold_6G00415980
rosa_rugosa Rorug03G0070700
rosa_wichuraiana Rw3G010800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 536
AccB7I CCANNNNNTGG 2 cut(s) 665, 920
AccI GTMKAC 1 cut(s) 147
AccII CGCG 2 cut(s) 828, 954
AciI CCGC 7 cut(s) 721, 784, 826, 828, 906, 952, 954
AclWI GGATC 3 cut(s) 86, 95, 905
AcoI YGGCCR 2 cut(s) 277, 922
AcuI CTGAAG 1 cut(s) 110
AcyI GRCGYC 1 cut(s) 885
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 4 cut(s) 208, 665, 727, 920
AflII CTTAAG 1 cut(s) 226
AflIII ACRYGT 1 cut(s) 710
AgsI TTSAA 4 cut(s) 23, 459, 581, 676
AluBI AGCT 8 cut(s) 26, 130, 225, 329, 649, 663, 736, 865
AluI AGCT 8 cut(s) 26, 130, 225, 329, 649, 663, 736, 865
Alw21I GWGCWC 2 cut(s) 867, 968
Alw26I GTCTC 1 cut(s) 529
AlwI GGATC 3 cut(s) 86, 95, 905
Ama87I CYCGRG 2 cut(s) 203, 727
AoxI GGCC 3 cut(s) 257, 277, 922
AspA2I CCTAGG 2 cut(s) 182, 415
AspLEI GCGC 1 cut(s) 905
AspS9I GGNCC 4 cut(s) 199, 236, 444, 601
AsuC2I CCSGG 3 cut(s) 210, 728, 729
AsuHPI GGTGA 4 cut(s) 79, 507, 560, 680
AsuII TTCGAA 1 cut(s) 751
AvaI CYCGRG 2 cut(s) 203, 727
AvaII GGWCC 4 cut(s) 199, 236, 444, 601
AvrII CCTAGG 2 cut(s) 182, 415
BalI TGGCCA 1 cut(s) 279
BanII GRGCYC 2 cut(s) 850, 867
BbsI GAAGAC 1 cut(s) 499
Bbv12I GWGCWC 2 cut(s) 867, 968
BccI CCATC 2 cut(s) 190, 211
BceAI ACGGC 1 cut(s) 746
BcnI CCSGG 3 cut(s) 210, 728, 729
BcoDI GTCTC 1 cut(s) 529
BfaI CTAG 3 cut(s) 183, 416, 758
BfmI CTRYAG 1 cut(s) 841
BfrI CTTAAG 1 cut(s) 226
BisI GCNGC 3 cut(s) 829, 906, 955
BlnI CCTAGG 2 cut(s) 182, 415
BlsI GCNGC 3 cut(s) 830, 907, 956
Bme1390I CCNGG 3 cut(s) 210, 728, 729
Bme18I GGWCC 4 cut(s) 199, 236, 444, 601
BmeT110I CYCGRG 2 cut(s) 203, 727
BmgT120I GGNCC 4 cut(s) 199, 236, 444, 601
BmiI GGNNCC 6 cut(s) 41, 289, 398, 445, 446, 832
BmrFI CCNGG 3 cut(s) 210, 728, 729
BmsI GCATC 2 cut(s) 331, 947
BpiI GAAGAC 1 cut(s) 499
Bpu10I CCTNAGC 1 cut(s) 861
Bpu14I TTCGAA 1 cut(s) 751
BpuEI CTTGAG 2 cut(s) 152, 629
BpuMI CCSGG 3 cut(s) 210, 728, 729
Bsa29I ATCGAT 2 cut(s) 90, 990
BsaHI GRCGYC 1 cut(s) 885
BsaJI CCNNGG 7 cut(s) 182, 209, 415, 727, 826, 919, 952
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 101, 131, 996, 1026
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 665, 727, 920
Bse1I ACTGG 1 cut(s) 238
BseCI ATCGAT 2 cut(s) 90, 990
BseDI CCNNGG 7 cut(s) 182, 209, 415, 727, 826, 919, 952
BseGI GGATG 1 cut(s) 771
BseLI CCNNNNNNNGG 4 cut(s) 208, 665, 727, 920
BseMII CTCAG 5 cut(s) 321, 363, 516, 540, 852
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 1 cut(s) 1004
Bsh1236I CGCG 2 cut(s) 828, 954
BshFI GGCC 3 cut(s) 259, 279, 924
BshVI ATCGAT 2 cut(s) 90, 990
BsiHKAI GWGCWC 2 cut(s) 867, 968
BsiHKCI CYCGRG 2 cut(s) 203, 727
BsiSI CCGG 5 cut(s) 99, 209, 260, 291, 728
BslFI GGGAC 5 cut(s) 226, 439, 457, 744, 896
BslI CCNNNNNNNGG 4 cut(s) 208, 665, 727, 920
BsmAI GTCTC 1 cut(s) 529
BsmFI GGGAC 5 cut(s) 226, 439, 457, 744, 896
BsnI GGCC 3 cut(s) 259, 279, 924
BsoBI CYCGRG 2 cut(s) 203, 727
Bsp119I TTCGAA 1 cut(s) 751
Bsp1286I GDGCHC 3 cut(s) 850, 867, 968
Bsp143I GATC 7 cut(s) 87, 91, 462, 745, 910, 987, 991
Bsp19I CCATGG 1 cut(s) 919
BspACI CCGC 7 cut(s) 721, 784, 826, 828, 906, 952, 954
BspANI GGCC 3 cut(s) 259, 279, 924
BspCNI CTCAG 5 cut(s) 322, 364, 515, 539, 853
BspDI ATCGAT 2 cut(s) 90, 990
BspFNI CGCG 2 cut(s) 828, 954
BspLI GGNNCC 6 cut(s) 41, 289, 398, 445, 446, 832
BspPI GGATC 3 cut(s) 86, 95, 905
BspT104I TTCGAA 1 cut(s) 751
BspTI CTTAAG 1 cut(s) 226
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 7 cut(s) 182, 209, 415, 727, 826, 919, 952
BssMI GATC 7 cut(s) 87, 91, 462, 745, 910, 987, 991
BssNI GRCGYC 1 cut(s) 885
BssT1I CCWWGG 3 cut(s) 182, 415, 919
Bst4CI ACNGT 1 cut(s) 535
Bst6I CTCTTC 1 cut(s) 465
BstACI GRCGYC 1 cut(s) 885
BstAFI CTTAAG 1 cut(s) 226
BstBI TTCGAA 1 cut(s) 751
BstDEI CTNAG 5 cut(s) 330, 372, 502, 526, 861
BstDSI CCRYGG 3 cut(s) 826, 919, 952
BstF5I GGATG 1 cut(s) 771
BstFNI CGCG 2 cut(s) 828, 954
BstHHI GCGC 1 cut(s) 905
BstKTI GATC 7 cut(s) 90, 94, 465, 748, 913, 990, 994
BstMAI GTCTC 1 cut(s) 529
BstMBI GATC 7 cut(s) 87, 91, 462, 745, 910, 987, 991
BstMWI GCNNNNNNNGC 1 cut(s) 963
BstNSI RCATGY 1 cut(s) 714
BstSCI CCNGG 3 cut(s) 208, 726, 727
BstSFI CTRYAG 1 cut(s) 841
BstUI CGCG 2 cut(s) 828, 954
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 1 cut(s) 910
BstYI RGATCY 1 cut(s) 910
Bsu15I ATCGAT 2 cut(s) 90, 990
BsuRI GGCC 3 cut(s) 259, 279, 924
BsuTUI ATCGAT 2 cut(s) 90, 990
BtgI CCRYGG 3 cut(s) 826, 919, 952
BtsCI GGATG 1 cut(s) 771
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 540
CfoI GCGC 1 cut(s) 905
Cfr13I GGNCC 4 cut(s) 199, 236, 444, 601
Cfr42I CCGCGG 2 cut(s) 829, 955
Cfr9I CCCGGG 1 cut(s) 727
ClaI ATCGAT 2 cut(s) 90, 990
CseI GACGC 1 cut(s) 893
Csp6I GTAC 1 cut(s) 475
CspCI CAANNNNNGTGG 4 cut(s) 860, 895, 957, 992
CviAII CATG 7 cut(s) 83, 598, 697, 711, 799, 895, 920
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 5 cut(s) 330, 372, 502, 526, 861
DpnI GATC 7 cut(s) 89, 93, 464, 747, 912, 989, 993
DpnII GATC 7 cut(s) 87, 91, 462, 745, 910, 987, 991
DrdI GACNNNNNNGTC 1 cut(s) 536
DseDI GACNNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 2 cut(s) 277, 922
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Ecl136II GAGCTC 1 cut(s) 865
Eco130I CCWWGG 3 cut(s) 182, 415, 919
Eco24I GRGCYC 2 cut(s) 850, 867
Eco47I GGWCC 4 cut(s) 199, 236, 444, 601
Eco53kI GAGCTC 1 cut(s) 865
Eco57I CTGAAG 1 cut(s) 110
Eco88I CYCGRG 2 cut(s) 203, 727
EcoICRI GAGCTC 1 cut(s) 865
EcoO109I RGGNCCY 1 cut(s) 444
EcoT14I CCWWGG 3 cut(s) 182, 415, 919
EcoT38I GRGCYC 2 cut(s) 850, 867
ErhI CCWWGG 3 cut(s) 182, 415, 919
FaeI CATG 7 cut(s) 86, 601, 700, 714, 802, 898, 923
FaqI GGGAC 5 cut(s) 226, 439, 457, 744, 896
FatI CATG 7 cut(s) 82, 597, 696, 710, 798, 894, 919
FauI CCCGC 2 cut(s) 791, 833
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 3 cut(s) 829, 906, 955
FokI GGATG 1 cut(s) 758
FriOI GRGCYC 2 cut(s) 850, 867
Fsp4HI GCNGC 3 cut(s) 829, 906, 955
FspBI CTAG 3 cut(s) 183, 416, 758
GlaI GCGC 1 cut(s) 904
GluI GCNGC 3 cut(s) 829, 906, 955
HaeIII GGCC 3 cut(s) 259, 279, 924
HapII CCGG 5 cut(s) 99, 209, 260, 291, 728
HgaI GACGC 1 cut(s) 893
HhaI GCGC 1 cut(s) 905
Hin1I GRCGYC 1 cut(s) 885
Hin1II CATG 7 cut(s) 86, 601, 700, 714, 802, 898, 923
Hin6I GCGC 1 cut(s) 903
HinP1I GCGC 1 cut(s) 903
HindIII AAGCTT 1 cut(s) 661
HinfI GANTC 3 cut(s) 705, 766, 1034
HpaII CCGG 5 cut(s) 99, 209, 260, 291, 728
HphI GGTGA 4 cut(s) 79, 507, 560, 680
Hpy166II GTNNAC 4 cut(s) 148, 389, 568, 979
Hpy188I TCNGA 3 cut(s) 529, 638, 745
Hpy188III TCNNGA 3 cut(s) 350, 400, 435
Hpy8I GTNNAC 4 cut(s) 148, 389, 568, 979
HpyAV CCTTC 3 cut(s) 134, 317, 787
HpyCH4III ACNGT 1 cut(s) 535
HpyCH4V TGCA 2 cut(s) 16, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 963
HpyF3I CTNAG 5 cut(s) 330, 372, 502, 526, 861
Hsp92I GRCGYC 1 cut(s) 885
Hsp92II CATG 7 cut(s) 86, 601, 700, 714, 802, 898, 923
HspAI GCGC 1 cut(s) 903
KflI GGGWCCC 1 cut(s) 444
KspI CCGCGG 2 cut(s) 829, 955
Kzo9I GATC 7 cut(s) 87, 91, 462, 745, 910, 987, 991
LmnI GCTCC 3 cut(s) 287, 326, 836
LweI GCATC 2 cut(s) 331, 947
MaeI CTAG 3 cut(s) 183, 416, 758
MaeIII GTNAC 2 cut(s) 74, 706
MalI GATC 7 cut(s) 89, 93, 464, 747, 912, 989, 993
MboI GATC 7 cut(s) 87, 91, 462, 745, 910, 987, 991
MboII GAAGA 5 cut(s) 183, 482, 499, 740, 787
MfeI CAATTG 2 cut(s) 298, 572
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 3 cut(s) 850, 867, 968
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 4 cut(s) 45, 298, 376, 572
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 1 cut(s) 714
Mox20I TGGCCA 1 cut(s) 279
MscI TGGCCA 1 cut(s) 279
MseI TTAA 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 695
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 2 cut(s) 828, 954
MspCI CTTAAG 1 cut(s) 226
MspI CCGG 5 cut(s) 99, 209, 260, 291, 728
MspR9I CCNGG 3 cut(s) 210, 728, 729
MunI CAATTG 2 cut(s) 298, 572
MvnI CGCG 2 cut(s) 828, 954
MwoI GCNNNNNNNGC 1 cut(s) 963
NciI CCSGG 3 cut(s) 210, 728, 729
NcoI CCATGG 1 cut(s) 919
NdeII GATC 7 cut(s) 87, 91, 462, 745, 910, 987, 991
NlaIII CATG 7 cut(s) 86, 601, 700, 714, 802, 898, 923
NlaIV GGNNCC 6 cut(s) 41, 289, 398, 445, 446, 832
NmuCI GTSAC 2 cut(s) 74, 706
NspI RCATGY 1 cut(s) 714
NspV TTCGAA 1 cut(s) 751
PaeR7I CTCGAG 1 cut(s) 203
PciI ACATGT 1 cut(s) 710
PcsI WCGNNNNNNNCGW 2 cut(s) 922, 987
PfeI GAWTC 2 cut(s) 766, 1034
PflFI GACNNNGTC 1 cut(s) 430
PflMI CCANNNNNTGG 2 cut(s) 665, 920
PkrI GCNGC 3 cut(s) 830, 907, 956
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
PpuMI RGGWCCY 1 cut(s) 444
PscI ACATGT 1 cut(s) 710
Psp124BI GAGCTC 1 cut(s) 867
Psp5II RGGWCCY 1 cut(s) 444
PspN4I GGNNCC 6 cut(s) 41, 289, 398, 445, 446, 832
PspPI GGNCC 4 cut(s) 199, 236, 444, 601
PspPPI RGGWCCY 1 cut(s) 444
PspXI VCTCGAGB 1 cut(s) 203
PsuI RGATCY 1 cut(s) 910
PsyI GACNNNGTC 1 cut(s) 430
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
RseI CAYNNNNRTG 1 cut(s) 695
SacI GAGCTC 1 cut(s) 867
SacII CCGCGG 2 cut(s) 829, 955
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 3 cut(s) 829, 906, 955
Sau3AI GATC 7 cut(s) 87, 91, 462, 745, 910, 987, 991
Sau96I GGNCC 4 cut(s) 199, 236, 444, 601
SchI GAGTC 1 cut(s) 714
ScrFI CCNGG 3 cut(s) 210, 728, 729
SduI GDGCHC 3 cut(s) 850, 867, 968
SfaNI GCATC 2 cut(s) 331, 947
SfcI CTRYAG 1 cut(s) 841
Sfr274I CTCGAG 1 cut(s) 203
Sfr303I CCGCGG 2 cut(s) 829, 955
SfuI TTCGAA 1 cut(s) 751
SgrBI CCGCGG 2 cut(s) 829, 955
SinI GGWCC 4 cut(s) 199, 236, 444, 601
SlaI CTCGAG 1 cut(s) 203
SmaI CCCGGG 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 4 cut(s) 131, 203, 226, 644
SmoI CTYRAG 4 cut(s) 131, 203, 226, 644
Sse9I AATT 4 cut(s) 45, 298, 376, 572
SsiI CCGC 7 cut(s) 721, 784, 826, 828, 906, 952, 954
SspMI CTAG 3 cut(s) 183, 416, 758
SstI GAGCTC 1 cut(s) 867
StyD4I CCNGG 3 cut(s) 208, 726, 727
StyI CCWWGG 3 cut(s) 182, 415, 919
TaaI ACNGT 1 cut(s) 535
TaqI TCGA 5 cut(s) 90, 204, 540, 751, 990
TasI AATT 4 cut(s) 45, 298, 376, 572
TauI GCSGC 3 cut(s) 831, 908, 957
TfiI GAWTC 2 cut(s) 766, 1034
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 2 cut(s) 195, 540
TseFI GTSAC 2 cut(s) 74, 706
Tsp45I GTSAC 2 cut(s) 74, 706
TspDTI ATGAA 3 cut(s) 171, 627, 787
TspMI CCCGGG 1 cut(s) 727
TspRI CASTG 2 cut(s) 195, 540
Tth111I GACNNNGTC 1 cut(s) 430
Van91I CCANNNNNTGG 2 cut(s) 665, 920
Vha464I CTTAAG 1 cut(s) 226
VpaK11BI GGWCC 4 cut(s) 199, 236, 444, 601
XceI RCATGY 1 cut(s) 714
XhoI CTCGAG 1 cut(s) 203
XmaI CCCGGG 1 cut(s) 727
XmaJI CCTAGG 2 cut(s) 182, 415
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 3 cut(s) 183, 416, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.