pycom04g13060

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012720
Physical Location & Seq
Reverse (-)
16068906 .. 16071204
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g13060.4

Sequence Viewer

Length: 1641 bp
ATGTGTATTTCAAATTTTATTTTATATTTTATTTTAATTCTTAAGGAAACTGTCCTCCAAATAAAATCTGAACCGTCCATTTCCAATCCCCACAACTCATATCCAACCCTTTCAAACAAAAACTCCCGCTTCTCCATTCAAATCCAACCGTTGCGACCCAAAAAGCCTACCTTTCTTATAAAATCCTCTCTCTCCGTCCCCACCCTTCTGTCTCATCCATTTCTCTCTGCGATTTTCTCACTGAGAGACCCACAAACCCTAACCCTTTCTGATTTTTCACCCCCCTTTCAACCAAAAATGCGTGAGATCCTTCACATCCAGGGAGGCCAGTGCGGCAACCAGATTGGCGCCAAGTTCTGGGAGGTGGTCTGCGCCGAGCACGGCATCGACGGCACCGGAAGGTACGAGGGAGACTCGGAGCTCCAGCTTGAAAGGATCAATGTCTACTACAACGAAGCCAGTTGCGGGAGGTACGTGCCGAGGGCTGTGCTCATGGACCTCGAGCCTGGGACCATGGACAGCGTCCGATCAGGACCCTACGGTCAGATTTTCCGGCCTGACAACTTCGTTTTCGGCCAGTCTGGTGCCGGAAACAACTGGGCCAAGGGTCACTATACCGAAGGCGCTGAGTTGATCGATTCGGTTCTTGATGTGGTTCGCAAGGAGGCCGAGAATTGTGATTGCCTACAAGGGTTTCAGGTGTGTCATTCTCTGGGAGGAGGTACTGGGTCTGGCATGGGTACTCTCTTGATTTCAAAGATCAGAGAGGAATACCCAGACCGTATGATGCTCACTTTCTCGGTTTTCCCCTCTCCAAAGGTCTCAGACACAGTTGTTGAGCCTTACAACGCAACCTTGTCTGTGCACCAGCTTGTTGAAAACGCAGACGAGTGTATGGTTCTTGATAATGAAGCTCTGTATGACATTTGCTTCCGCACACTCAAGCTCAGCACCCCGAGCTTTGGGGATTTGAACCATTTGATTTCTGCAACTATGTCTGGTGTCACATGCTGTTTGAGGTTCCCTGGTCAGTTGAACTCTGACCTCCGAAAGCTTGCTGTCAATCTCATCCCCTTCCCTCGTCTTCACTTCTTCATGGTGGGTTTTGCTCCTTTGACCTCACGCGGTTCACAGCAGTACAGAGCTCTCACTGTGCCTGAGCTCACACAGCAAATGTGGGATGCGAAGAACATGATGTGTGCAGCTGACCCTCGCCATGGTCGGTATTTGACTGCCTCAGCCGTGTTCAGGGGTAAAATGAGCACAAAGGAAGTTGATGAACAGATGATCAATGTCCAGAACAAGAACTCTTCTTACTTTGTGGAATGGATTCCCAACAATGTCAAGTCTACTGTATGTGATATCCCACCCACCGGCTTGAAAATGGCGTCCACCTTCATTGGAAACTCAACCTCAATTCAGGAGATGTTCCGCCGTGTGAGCGAGCAGTTCACTGCTATGTTCAGGAGGAAGGCTTTCTTGCATTGGTACACTGGTGAAGGCATGGACGAGATGGAGTTCACAGAAGCTGAAAGCAACATGAATGATCTGGTTTCAGAGTATCAGCAGTACCAGGATGCCACAGCTGAGGAGGATGAGTACTATGAGGACGAAGAGGAGGAAGCTCAGGAGGAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

547

Amino Acids

61.58

Weight (kDa)

5.04

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62690 AT5G62700
fragaria_vesca FvH4_6g12290
malus_domestica MD04G1143700.v1.1 MD12G1158100.v1.1
prunus_persica Prupe.6G268300_v2.0.a1
pyrus_communis pycom04g13060 pycom05g25220 pycom06g07900 pycom07g24790 pycom11g07310 pycom12g15020
rosa_chinensis RchiOBHm_Chr3g0464121
rosa_laevigata RLG00000024721
rosa_multiflora Rmu_sc0010401.1_g000003
rosa_roxburghii Rroxscaffold_6G00415980
rosa_rugosa Rorug03G0070700
rosa_wichuraiana Rw3G010800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 179
AasI GACNNNNNNGTC 1 cut(s) 540
AccB1I GGYRCC 3 cut(s) 347, 392, 584
AccB7I CCANNNNNTGG 1 cut(s) 357
AccI GTMKAC 2 cut(s) 444, 1349
AccII CGCG 1 cut(s) 1125
AciI CCGC 6 cut(s) 127, 333, 465, 934, 1125, 1432
AclWI GGATC 2 cut(s) 301, 443
AcoI YGGCCR 1 cut(s) 574
AcsI RAATTY 1 cut(s) 13
AcyI GRCGYC 2 cut(s) 348, 1388
AfaI GTAC 8 cut(s) 404, 473, 724, 742, 1139, 1490, 1571, 1601
AfiI CCNNNNNNNGG 6 cut(s) 357, 465, 962, 1217, 1248, 1373
AflII CTTAAG 1 cut(s) 41
AjnI CCWGG 4 cut(s) 318, 505, 1024, 1572
Alw21I GWGCWC 7 cut(s) 381, 423, 492, 867, 1147, 1164, 1265
Alw26I GTCTC 4 cut(s) 216, 240, 405, 826
Alw44I GTGCAC 1 cut(s) 863
AlwI GGATC 2 cut(s) 301, 443
AlwNI CAGNNNCTG 1 cut(s) 1529
Ama87I CYCGRG 2 cut(s) 500, 955
AoxI GGCC 5 cut(s) 325, 554, 574, 600, 666
ApaLI GTGCAC 1 cut(s) 863
ApeKI GCWGC 1 cut(s) 1202
ApoI RAATTY 1 cut(s) 13
Asp700I GAANNNNTTC 2 cut(s) 1329, 1475
AspLEI GCGC 3 cut(s) 350, 374, 626
AspS9I GGNCC 4 cut(s) 496, 510, 533, 600
AsuHPI GGTGA 2 cut(s) 270, 1508
AvaI CYCGRG 2 cut(s) 500, 955
AvaII GGWCC 3 cut(s) 496, 510, 533
BaeGI GKGCMC 1 cut(s) 867
BaeI ACNNNNGTAYC 2 cut(s) 1480, 1513
BanI GGYRCC 3 cut(s) 347, 392, 584
BanII GRGCYC 3 cut(s) 423, 1147, 1164
BbsI GAAGAC 1 cut(s) 1076
Bbv12I GWGCWC 7 cut(s) 381, 423, 492, 867, 1147, 1164, 1265
BbvCI CCTCAGC 2 cut(s) 1237, 1587
BbvI GCAGC 1 cut(s) 1214
BccI CCATC 1 cut(s) 1507
BceAI ACGGC 4 cut(s) 397, 406, 1226, 1419
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 4 cut(s) 320, 507, 1026, 1574
BclI TGATCA 1 cut(s) 1287
BcoDI GTCTC 4 cut(s) 216, 240, 405, 826
BfoI RGCGCY 2 cut(s) 351, 627
BfrI CTTAAG 1 cut(s) 41
BglI GCCNNNNNGGC 1 cut(s) 333
BisI GCNGC 2 cut(s) 334, 1203
BlpI GCTNAGC 1 cut(s) 947
BlsI GCNGC 2 cut(s) 335, 1204
BmcAI AGTACT 1 cut(s) 1601
Bme1390I CCNGG 4 cut(s) 320, 507, 1026, 1574
Bme18I GGWCC 3 cut(s) 496, 510, 533
BmeT110I CYCGRG 2 cut(s) 500, 955
BmgT120I GGNCC 4 cut(s) 496, 510, 533, 600
BmiI GGNNCC 6 cut(s) 349, 394, 511, 535, 586, 1022
BmrFI CCNGG 4 cut(s) 320, 507, 1026, 1574
BmrI ACTGGG 2 cut(s) 607, 735
BmsI GCATC 4 cut(s) 393, 777, 1171, 1567
BmuI ACTGGG 2 cut(s) 607, 735
BpiI GAAGAC 1 cut(s) 1076
BplI GAGNNNNNCTC 2 cut(s) 398, 430
BpmI CTGGAG 1 cut(s) 407
Bpu10I CCTNAGC 4 cut(s) 1158, 1237, 1587, 1626
Bpu1102I GCTNAGC 1 cut(s) 947
BpuEI CTTGAG 1 cut(s) 926
Bsa29I ATCGAT 1 cut(s) 636
BsaAI YACGTR 1 cut(s) 475
BsaHI GRCGYC 2 cut(s) 348, 1388
BsaI GGTCTC 2 cut(s) 240, 826
BsaJI CCNNGG 7 cut(s) 319, 479, 506, 513, 603, 1024, 1216
BsaWI WCCGGW 1 cut(s) 395
BsaXI ACNNNNNCTCC 5 cut(s) 107, 137, 1583, 1613, 1622
Bsc4I CCNNNNNNNGG 6 cut(s) 357, 465, 962, 1217, 1248, 1373
Bse118I RCCGGY 1 cut(s) 1373
Bse1I ACTGG 6 cut(s) 328, 459, 577, 602, 730, 1498
BseBI CCWGG 4 cut(s) 320, 507, 1026, 1574
BseCI ATCGAT 1 cut(s) 636
BseDI CCNNGG 7 cut(s) 319, 479, 506, 513, 603, 1024, 1216
BseGI GGATG 6 cut(s) 214, 315, 1068, 1186, 1582, 1600
BseLI CCNNNNNNNGG 6 cut(s) 357, 465, 962, 1217, 1248, 1373
BseMII CTCAG 8 cut(s) 233, 618, 837, 961, 1149, 1251, 1578, 1640
BseNI ACTGG 6 cut(s) 328, 459, 577, 602, 730, 1498
BseRI GAGGAG 3 cut(s) 732, 1604, 1631
BseSI GKGCMC 1 cut(s) 867
BseXI GCAGC 1 cut(s) 1214
BsgI GTGCAG 1 cut(s) 1221
Bsh1236I CGCG 1 cut(s) 1125
BshFI GGCC 5 cut(s) 327, 556, 576, 602, 668
BshNI GGYRCC 3 cut(s) 347, 392, 584
BshVI ATCGAT 1 cut(s) 636
BsiHKAI GWGCWC 7 cut(s) 381, 423, 492, 867, 1147, 1164, 1265
BsiHKCI CYCGRG 2 cut(s) 500, 955
BsiSI CCGG 4 cut(s) 396, 553, 588, 1374
BslFI GGGAC 2 cut(s) 182, 523
BslI CCNNNNNNNGG 6 cut(s) 357, 465, 962, 1217, 1248, 1373
BsmAI GTCTC 4 cut(s) 216, 240, 405, 826
BsmFI GGGAC 2 cut(s) 182, 523
BsnI GGCC 5 cut(s) 327, 556, 576, 602, 668
Bso31I GGTCTC 2 cut(s) 240, 826
BsoBI CYCGRG 2 cut(s) 500, 955
Bsp1286I GDGCHC 7 cut(s) 381, 423, 492, 867, 1147, 1164, 1265
Bsp143I GATC 7 cut(s) 306, 435, 527, 633, 759, 1287, 1546
Bsp1720I GCTNAGC 1 cut(s) 947
Bsp19I CCATGG 2 cut(s) 513, 1216
BspACI CCGC 6 cut(s) 127, 333, 465, 934, 1125, 1432
BspANI GGCC 5 cut(s) 327, 556, 576, 602, 668
BspCNI CTCAG 8 cut(s) 234, 619, 836, 960, 1150, 1250, 1579, 1639
BspDI ATCGAT 1 cut(s) 636
BspFNI CGCG 1 cut(s) 1125
BspLI GGNNCC 6 cut(s) 349, 394, 511, 535, 586, 1022
BspPI GGATC 2 cut(s) 301, 443
BspT107I GGYRCC 3 cut(s) 347, 392, 584
BspTI CTTAAG 1 cut(s) 41
BspTNI GGTCTC 2 cut(s) 240, 826
BsrFI RCCGGY 1 cut(s) 1373
BsrI ACTGG 6 cut(s) 328, 459, 577, 602, 730, 1498
BssAI RCCGGY 1 cut(s) 1373
BssECI CCNNGG 7 cut(s) 319, 479, 506, 513, 603, 1024, 1216
BssMI GATC 7 cut(s) 306, 435, 527, 633, 759, 1287, 1546
BssNI GRCGYC 2 cut(s) 348, 1388
BssT1I CCWWGG 3 cut(s) 513, 603, 1216
Bst2UI CCWGG 4 cut(s) 320, 507, 1026, 1574
Bst4CI ACNGT 8 cut(s) 52, 75, 150, 542, 782, 832, 1153, 1354
Bst6I CTCTTC 2 cut(s) 1315, 1608
BstACI GRCGYC 2 cut(s) 348, 1388
BstAFI CTTAAG 1 cut(s) 41
BstBAI YACGTR 1 cut(s) 475
BstC8I GCNNGC 2 cut(s) 1056, 1445
BstDEI CTNAG 8 cut(s) 242, 627, 823, 947, 1158, 1237, 1587, 1626
BstDSI CCRYGG 2 cut(s) 513, 1216
BstF5I GGATG 6 cut(s) 214, 315, 1068, 1186, 1582, 1600
BstFNI CGCG 1 cut(s) 1125
BstH2I RGCGCY 2 cut(s) 351, 627
BstHHI GCGC 3 cut(s) 350, 374, 626
BstKTI GATC 7 cut(s) 309, 438, 530, 636, 762, 1290, 1549
BstMAI GTCTC 4 cut(s) 216, 240, 405, 826
BstMBI GATC 7 cut(s) 306, 435, 527, 633, 759, 1287, 1546
BstMWI GCNNNNNNNGC 5 cut(s) 333, 390, 957, 1168, 1440
BstNI CCWGG 4 cut(s) 320, 507, 1026, 1574
BstNSI RCATGY 1 cut(s) 1011
BstSCI CCNGG 4 cut(s) 318, 505, 1024, 1572
BstSLI GKGCMC 1 cut(s) 867
BstUI CGCG 1 cut(s) 1125
BstV1I GCAGC 1 cut(s) 1214
BstV2I GAAGAC 1 cut(s) 1076
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
Bsu15I ATCGAT 1 cut(s) 636
BsuRI GGCC 5 cut(s) 327, 556, 576, 602, 668
BsuTUI ATCGAT 1 cut(s) 636
BtgI CCRYGG 2 cut(s) 513, 1216
BtsCI GGATG 6 cut(s) 214, 315, 1068, 1186, 1582, 1600
BtsI GCAGTG 1 cut(s) 1452
BtsIMutI CAGTG 5 cut(s) 239, 335, 1149, 1452, 1491
Cac8I GCNNGC 2 cut(s) 1056, 1445
CaiI CAGNNNCTG 1 cut(s) 1529
CfoI GCGC 3 cut(s) 350, 374, 626
Cfr10I RCCGGY 1 cut(s) 1373
Cfr13I GGNCC 4 cut(s) 496, 510, 533, 600
ClaI ATCGAT 1 cut(s) 636
CseI GACGC 2 cut(s) 511, 1377
Csp6I GTAC 8 cut(s) 403, 472, 723, 741, 1138, 1489, 1570, 1600
CspCI CAANNNNNGTGG 2 cut(s) 1381, 1416
CviAII CATG 9 cut(s) 493, 514, 736, 1008, 1096, 1192, 1217, 1504, 1540
CviQI GTAC 8 cut(s) 403, 472, 723, 741, 1138, 1489, 1570, 1600
DdeI CTNAG 8 cut(s) 242, 627, 823, 947, 1158, 1237, 1587, 1626
DinI GGCGCC 1 cut(s) 349
DpnI GATC 7 cut(s) 308, 437, 529, 635, 761, 1289, 1548
DpnII GATC 7 cut(s) 306, 435, 527, 633, 759, 1287, 1546
DrdI GACNNNNNNGTC 1 cut(s) 540
DseDI GACNNNNNNGTC 1 cut(s) 540
EaeI YGGCCR 1 cut(s) 574
Eam1104I CTCTTC 2 cut(s) 1315, 1608
EarI CTCTTC 2 cut(s) 1315, 1608
EciI GGCGGA 1 cut(s) 1421
Ecl136II GAGCTC 3 cut(s) 421, 1145, 1162
Eco130I CCWWGG 3 cut(s) 513, 603, 1216
Eco24I GRGCYC 3 cut(s) 423, 1147, 1164
Eco31I GGTCTC 2 cut(s) 240, 826
Eco32I GATATC 1 cut(s) 1363
Eco47I GGWCC 3 cut(s) 496, 510, 533
Eco53kI GAGCTC 3 cut(s) 421, 1145, 1162
Eco88I CYCGRG 2 cut(s) 500, 955
EcoICRI GAGCTC 3 cut(s) 421, 1145, 1162
EcoO109I RGGNCCY 1 cut(s) 533
EcoRII CCWGG 4 cut(s) 318, 505, 1024, 1572
EcoRV GATATC 1 cut(s) 1363
EcoT14I CCWWGG 3 cut(s) 513, 603, 1216
EcoT38I GRGCYC 3 cut(s) 423, 1147, 1164
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
ErhI CCWWGG 3 cut(s) 513, 603, 1216
FaeI CATG 9 cut(s) 496, 517, 739, 1011, 1099, 1195, 1220, 1507, 1543
FalI AAGNNNNNCTT 4 cut(s) 155, 187, 1463, 1495
FaqI GGGAC 2 cut(s) 182, 523
FatI CATG 9 cut(s) 492, 513, 735, 1007, 1095, 1191, 1216, 1503, 1539
FauI CCCGC 2 cut(s) 134, 458
FbaI TGATCA 1 cut(s) 1287
FblI GTMKAC 2 cut(s) 444, 1349
Fnu4HI GCNGC 2 cut(s) 334, 1203
FokI GGATG 6 cut(s) 201, 302, 1055, 1193, 1589, 1607
FriOI GRGCYC 3 cut(s) 423, 1147, 1164
Fsp4HI GCNGC 2 cut(s) 334, 1203
GlaI GCGC 3 cut(s) 349, 373, 625
GluI GCNGC 2 cut(s) 334, 1203
GsuI CTGGAG 1 cut(s) 407
HaeII RGCGCY 2 cut(s) 351, 627
HaeIII GGCC 5 cut(s) 327, 556, 576, 602, 668
HapII CCGG 4 cut(s) 396, 553, 588, 1374
HgaI GACGC 2 cut(s) 511, 1377
HhaI GCGC 3 cut(s) 350, 374, 626
Hin1I GRCGYC 2 cut(s) 348, 1388
Hin1II CATG 9 cut(s) 496, 517, 739, 1011, 1099, 1195, 1220, 1507, 1543
Hin6I GCGC 3 cut(s) 348, 372, 624
HinP1I GCGC 3 cut(s) 348, 372, 624
HindIII AAGCTT 1 cut(s) 1052
HinfI GANTC 3 cut(s) 413, 638, 1330
HpaII CCGG 4 cut(s) 396, 553, 588, 1374
HphI GGTGA 2 cut(s) 270, 1508
Hpy166II GTNNAC 8 cut(s) 445, 865, 1130, 1350, 1392, 1452, 1491, 1521
Hpy188III TCNNGA 8 cut(s) 531, 647, 748, 902, 1297, 1421, 1465, 1628
Hpy8I GTNNAC 8 cut(s) 445, 865, 1130, 1350, 1392, 1452, 1491, 1521
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 8 cut(s) 215, 320, 393, 614, 1084, 1405, 1465, 1493
HpyCH4III ACNGT 8 cut(s) 52, 75, 150, 542, 782, 832, 1153, 1354
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 4 cut(s) 865, 989, 1202, 1483
HpyF10VI GCNNNNNNNGC 5 cut(s) 333, 390, 957, 1168, 1440
HpyF3I CTNAG 8 cut(s) 242, 627, 823, 947, 1158, 1237, 1587, 1626
HpySE526I ACGT 1 cut(s) 474
Hsp92I GRCGYC 2 cut(s) 348, 1388
Hsp92II CATG 9 cut(s) 496, 517, 739, 1011, 1099, 1195, 1220, 1507, 1543
HspAI GCGC 3 cut(s) 348, 372, 624
KasI GGCGCC 1 cut(s) 347
Ksp22I TGATCA 1 cut(s) 1287
Kzo9I GATC 7 cut(s) 306, 435, 527, 633, 759, 1287, 1546
LmnI GCTCC 3 cut(s) 418, 426, 1114
Lsp1109I GCAGC 1 cut(s) 1214
LweI GCATC 4 cut(s) 393, 777, 1171, 1567
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 2 cut(s) 608, 1003
MalI GATC 7 cut(s) 308, 437, 529, 635, 761, 1289, 1548
MboI GATC 7 cut(s) 306, 435, 527, 633, 759, 1287, 1546
MboII GAAGA 5 cut(s) 1076, 1084, 1198, 1302, 1625
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 7 cut(s) 381, 423, 492, 867, 1147, 1164, 1265
MluCI AATT 4 cut(s) 13, 36, 673, 1416
Mly113I GGCGCC 1 cut(s) 348
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 2 cut(s) 128, 169
MroXI GAANNNNTTC 2 cut(s) 1329, 1475
MseI TTAA 2 cut(s) 35, 42
MslI CAYNNNNRTG 1 cut(s) 1457
MspA1I CMGCKG 2 cut(s) 1205, 1586
MspCI CTTAAG 1 cut(s) 41
MspI CCGG 4 cut(s) 396, 553, 588, 1374
MspR9I CCNGG 4 cut(s) 320, 507, 1026, 1574
MvaI CCWGG 4 cut(s) 320, 507, 1026, 1574
MvnI CGCG 1 cut(s) 1125
MwoI GCNNNNNNNGC 5 cut(s) 333, 390, 957, 1168, 1440
NarI GGCGCC 1 cut(s) 348
NcoI CCATGG 2 cut(s) 513, 1216
NdeII GATC 7 cut(s) 306, 435, 527, 633, 759, 1287, 1546
NlaIII CATG 9 cut(s) 496, 517, 739, 1011, 1099, 1195, 1220, 1507, 1543
NlaIV GGNNCC 6 cut(s) 349, 394, 511, 535, 586, 1022
NmeAIII GCCGAG 3 cut(s) 400, 504, 694
NmuCI GTSAC 2 cut(s) 608, 1003
NspI RCATGY 1 cut(s) 1011
PaeR7I CTCGAG 1 cut(s) 500
PdmI GAANNNNTTC 2 cut(s) 1329, 1475
PfeI GAWTC 2 cut(s) 638, 1330
PflFI GACNNNGTC 1 cut(s) 521
PflMI CCANNNNNTGG 1 cut(s) 357
PkrI GCNGC 2 cut(s) 335, 1204
PleI GAGTC 1 cut(s) 407
PluTI GGCGCC 1 cut(s) 351
PpsI GAGTC 1 cut(s) 407
Ppu21I YACGTR 1 cut(s) 475
PpuMI RGGWCCY 1 cut(s) 533
PsiI TTATAA 1 cut(s) 179
Psp124BI GAGCTC 3 cut(s) 423, 1147, 1164
Psp5II RGGWCCY 1 cut(s) 533
Psp6I CCWGG 4 cut(s) 318, 505, 1024, 1572
PspGI CCWGG 4 cut(s) 318, 505, 1024, 1572
PspN4I GGNNCC 6 cut(s) 349, 394, 511, 535, 586, 1022
PspPI GGNCC 4 cut(s) 496, 510, 533, 600
PspPPI RGGWCCY 1 cut(s) 533
PspXI VCTCGAGB 1 cut(s) 500
PsrI GAACNNNNNNTAC 2 cut(s) 1298, 1330
PstNI CAGNNNCTG 1 cut(s) 1529
PsuI RGATCY 1 cut(s) 306
PsyI GACNNNGTC 1 cut(s) 521
PvuII CAGCTG 2 cut(s) 1205, 1586
RsaI GTAC 8 cut(s) 404, 473, 724, 742, 1139, 1490, 1571, 1601
RsaNI GTAC 8 cut(s) 403, 472, 723, 741, 1138, 1489, 1570, 1600
RseI CAYNNNNRTG 1 cut(s) 1457
SacI GAGCTC 3 cut(s) 423, 1147, 1164
SaqAI TTAA 2 cut(s) 35, 42
SatI GCNGC 2 cut(s) 334, 1203
Sau3AI GATC 7 cut(s) 306, 435, 527, 633, 759, 1287, 1546
Sau96I GGNCC 4 cut(s) 496, 510, 533, 600
ScaI AGTACT 1 cut(s) 1601
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 4 cut(s) 320, 507, 1026, 1574
SduI GDGCHC 7 cut(s) 381, 423, 492, 867, 1147, 1164, 1265
SfaNI GCATC 4 cut(s) 393, 777, 1171, 1567
SfoI GGCGCC 1 cut(s) 349
Sfr274I CTCGAG 1 cut(s) 500
SinI GGWCC 3 cut(s) 496, 510, 533
SlaI CTCGAG 1 cut(s) 500
SmiMI CAYNNNNRTG 1 cut(s) 1457
SmlI CTYRAG 3 cut(s) 41, 500, 941
SmoI CTYRAG 3 cut(s) 41, 500, 941
Sse9I AATT 4 cut(s) 13, 36, 673, 1416
SsiI CCGC 6 cut(s) 127, 333, 465, 934, 1125, 1432
SspDI GGCGCC 1 cut(s) 347
SstI GAGCTC 3 cut(s) 423, 1147, 1164
StyD4I CCNGG 4 cut(s) 318, 505, 1024, 1572
StyI CCWWGG 3 cut(s) 513, 603, 1216
TaaI ACNGT 8 cut(s) 52, 75, 150, 542, 782, 832, 1153, 1354
TaiI ACGT 1 cut(s) 477
TaqI TCGA 3 cut(s) 387, 501, 636
TasI AATT 4 cut(s) 13, 36, 673, 1416
TatI WGTACW 2 cut(s) 1137, 1599
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 2 cut(s) 638, 1330
Tru1I TTAA 2 cut(s) 35, 42
Tru9I TTAA 2 cut(s) 35, 42
TscAI CASTG 5 cut(s) 246, 335, 1156, 1459, 1498
TseFI GTSAC 2 cut(s) 608, 1003
TseI GCWGC 1 cut(s) 1202
Tsp45I GTSAC 2 cut(s) 608, 1003
TspDTI ATGAA 5 cut(s) 924, 1084, 1293, 1387, 1556
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 5 cut(s) 246, 335, 1156, 1459, 1498
Tth111I GACNNNGTC 1 cut(s) 521
Van91I CCANNNNNTGG 1 cut(s) 357
Vha464I CTTAAG 1 cut(s) 41
VneI GTGCAC 1 cut(s) 863
VpaK11BI GGWCC 3 cut(s) 496, 510, 533
XapI RAATTY 1 cut(s) 13
XceI RCATGY 1 cut(s) 1011
XhoI CTCGAG 1 cut(s) 500
XmiI GTMKAC 2 cut(s) 444, 1349
XmnI GAANNNNTTC 2 cut(s) 1329, 1475
ZrmI AGTACT 1 cut(s) 1601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.