MD12G1158100.v1.1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012720
Physical Location & Seq
Reverse (-)
23874765 .. 23877277
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1158100.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGCGTGAGATTCTTCATATCCAGGGAGGCCAGTGCGGCAACCAGATCGGCGCCAAGTTCTGGGAGGTGGTCTGCGCCGAGCACGGCATCGACGGCACCGGAAGGTACGAGGGAGACGCGGAGCTCCAGCTTGAGAGGATCAATGTCTACTACAACGAAGCCAGTTGCGGGAGGTACGTGCCGAGGGCTGTGCTCATGGACCTTGAGCCTGGGACCATGGACAGCGTCAGATCTGGACCCTACGGTCAGATCTTCAGGCCTGATAACTTCGTTTTCGGCCAGTCTGGTGCCGGAAACAACTGGGCGAAGGGTCACTACACCGAGGGCGCGGAGTTGATCGATTCTGTTCTCGATGTGGTTCGCAAGGAGGCTGAGAATTGTGATTGCCTGCAAGGGTTTCAGGTCTGCCATTCTCTAGGAGGAGGTACTGGGTCTGGCATGGGTACTCTGTTGATTTCAAAGATCAGAGAGGAATACCCAGATCGGATGATGCTCACTTTCTCGGTCTTCCCCTCCCCTAAGGTCTCAGACACAGTTGTTGAGCCCTACAATGCAACCCTGTCTGTGCACCAGCTGGTCGAGAATGCGGATGAGTGTATGGTCCTTGATAACGAAGCTCTTTACGACATTTGCTTCCGCACACTCAAGCTCAGCACCCCAAGCTTTGGAGATTTGAACCATTTGATTTCTGCAACTATGTCTGGTGTCACATGCTGTTTGAGGTTCCCTGGTCAGTTGAACTCTGACCTTCGAAAGCTTGCTGTCAATCTCATCCCCTTCCCTCGTCTCCACTTCTTCATGGTGGGTTTTGCTCCTTTGACGTCACGTGGTTCACAGCAGTATAGATCTCTCACAGTGCCTGAGCTCACACAGCAAATGTGGGATTCAAAGAACATGATGTGCGCAGCTGACCCTCGTCATGGTCGGTATCTGACTGCCTCTGCCATGTTCAGGGGTAAAATGAGCACTAAGGAGGTTGATGAACAGATGATCAATGTCCAGAACAAGAACTCCTCTTACTTTGTGGAATGGATTCCCCACAATGTCAAGTCAACTGTCTGTGACATCCCTCCCACCGGCTTGCAAATGGCATCCACCTTCATTGGAAACTCGACCTCAATTCAGGAGATGTTCCGCCGTGTGAGCGAGCAGTTCACTGCTATGTTCAGGAGGAAGGCTTTCTTGCATTGGTACACTGGTGAAGGCATGGACGAGATGGAGTTCACAGAAGCTGAAAGCAACATGAATGACCTGGTTTCAGAGTATCAGCAGTACCAGGATGCCACAGCTGAGGAGGATGAATACTATGAGGACGAGGAGGAGGAAGCTCAGGAGGAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

448

Amino Acids

50.42

Weight (kDa)

4.71

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 211 1.2e-64 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 261 - 382 2.1e-40 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62690 AT5G62700
fragaria_vesca FvH4_6g12290
malus_domestica MD04G1143700.v1.1 MD12G1158100.v1.1
prunus_persica Prupe.6G268300_v2.0.a1
pyrus_communis pycom04g13060 pycom05g25220 pycom06g07900 pycom07g24790 pycom11g07310 pycom12g15020
rosa_chinensis RchiOBHm_Chr3g0464121
rosa_laevigata RLG00000024721
rosa_multiflora Rmu_sc0010401.1_g000003
rosa_roxburghii Rroxscaffold_6G00415980
rosa_rugosa Rorug03G0070700
rosa_wichuraiana Rw3G010800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 243
AatII GACGTC 1 cut(s) 824
Acc16I TGCGCA 1 cut(s) 904
AccB1I GGYRCC 3 cut(s) 50, 95, 287
AccB7I CCANNNNNTGG 2 cut(s) 60, 665
AccI GTMKAC 1 cut(s) 147
AccII CGCG 2 cut(s) 119, 329
AciI CCGC 7 cut(s) 36, 119, 168, 329, 587, 637, 1135
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 277
AcuI CTGAAG 1 cut(s) 238
AcvI CACGTG 1 cut(s) 827
AcyI GRCGYC 2 cut(s) 51, 821
AfaI GTAC 6 cut(s) 107, 176, 427, 445, 1193, 1274
AfiI CCNNNNNNNGG 6 cut(s) 60, 168, 665, 920, 951, 1076
AgsI TTSAA 4 cut(s) 459, 676, 739, 888
AjnI CCWGG 5 cut(s) 21, 208, 727, 1251, 1275
AloI GAACNNNNNNTCC 2 cut(s) 995, 1027
Alw21I GWGCWC 6 cut(s) 84, 126, 195, 570, 867, 968
Alw26I GTCTC 3 cut(s) 108, 529, 791
Alw44I GTGCAC 1 cut(s) 566
AlwI GGATC 1 cut(s) 146
AlwNI CAGNNNCTG 2 cut(s) 860, 1232
AoxI GGCC 3 cut(s) 28, 257, 277
ApaLI GTGCAC 1 cut(s) 566
ApeKI GCWGC 1 cut(s) 905
Asp700I GAANNNNTTC 2 cut(s) 1032, 1178
AspLEI GCGC 4 cut(s) 53, 77, 329, 905
AspS9I GGNCC 4 cut(s) 199, 213, 236, 601
AsuHPI GGTGA 1 cut(s) 1211
AsuII TTCGAA 1 cut(s) 751
AvaII GGWCC 4 cut(s) 199, 213, 236, 601
AxyI CCTNAGG 1 cut(s) 519
BaeGI GKGCMC 1 cut(s) 570
BaeI ACNNNNGTAYC 2 cut(s) 1183, 1216
BanI GGYRCC 3 cut(s) 50, 95, 287
BanII GRGCYC 3 cut(s) 126, 546, 867
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
BbrPI CACGTG 1 cut(s) 827
BbsI GAAGAC 1 cut(s) 499
Bbv12I GWGCWC 6 cut(s) 84, 126, 195, 570, 867, 968
BbvCI CCTCAGC 1 cut(s) 1290
BbvI GCAGC 1 cut(s) 917
BccI CCATC 1 cut(s) 1210
BceAI ACGGC 3 cut(s) 100, 109, 1122
BcgI CGANNNNNNTGC 4 cut(s) 28, 62, 172, 206
BciT130I CCWGG 5 cut(s) 23, 210, 729, 1253, 1277
BclI TGATCA 1 cut(s) 990
BcoDI GTCTC 3 cut(s) 108, 529, 791
BfaI CTAG 1 cut(s) 416
BfoI RGCGCY 1 cut(s) 54
BglI GCCNNNNNGGC 1 cut(s) 36
BglII AGATCT 3 cut(s) 230, 249, 845
BisI GCNGC 2 cut(s) 37, 906
BlpI GCTNAGC 1 cut(s) 650
BlsI GCNGC 2 cut(s) 38, 907
Bme1390I CCNGG 5 cut(s) 23, 210, 729, 1253, 1277
Bme18I GGWCC 4 cut(s) 199, 213, 236, 601
BmgT120I GGNCC 4 cut(s) 199, 213, 236, 601
BmiI GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
BmrFI CCNGG 5 cut(s) 23, 210, 729, 1253, 1277
BmrI ACTGGG 2 cut(s) 310, 438
BmsI GCATC 4 cut(s) 96, 480, 1100, 1270
BmuI ACTGGG 2 cut(s) 310, 438
BoxI GACNNNNGTC 1 cut(s) 915
BpiI GAAGAC 1 cut(s) 499
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 3 cut(s) 861, 1290, 1329
Bpu1102I GCTNAGC 1 cut(s) 650
Bpu14I TTCGAA 1 cut(s) 751
BpuEI CTTGAG 3 cut(s) 152, 224, 629
Bsa29I ATCGAT 1 cut(s) 339
BsaAI YACGTR 2 cut(s) 178, 827
BsaHI GRCGYC 2 cut(s) 51, 821
BsaI GGTCTC 1 cut(s) 529
BsaJI CCNNGG 6 cut(s) 22, 182, 209, 216, 321, 727
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 3 cut(s) 995, 1025, 1325
Bsc4I CCNNNNNNNGG 6 cut(s) 60, 168, 665, 920, 951, 1076
Bse118I RCCGGY 1 cut(s) 1076
Bse1I ACTGG 6 cut(s) 31, 162, 280, 305, 433, 1201
Bse21I CCTNAGG 1 cut(s) 519
BseBI CCWGG 5 cut(s) 23, 210, 729, 1253, 1277
BseCI ATCGAT 1 cut(s) 339
BseDI CCNNGG 6 cut(s) 22, 182, 209, 216, 321, 727
BseGI GGATG 7 cut(s) 492, 595, 771, 1065, 1091, 1285, 1303
BseLI CCNNNNNNNGG 6 cut(s) 60, 168, 665, 920, 951, 1076
BseMII CTCAG 6 cut(s) 363, 540, 664, 852, 1281, 1343
BseNI ACTGG 6 cut(s) 31, 162, 280, 305, 433, 1201
BseRI GAGGAG 5 cut(s) 435, 1003, 1307, 1331, 1334
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 1 cut(s) 917
Bsh1236I CGCG 2 cut(s) 119, 329
BshFI GGCC 3 cut(s) 30, 259, 279
BshNI GGYRCC 3 cut(s) 50, 95, 287
BshVI ATCGAT 1 cut(s) 339
BsiHKAI GWGCWC 6 cut(s) 84, 126, 195, 570, 867, 968
BsiSI CCGG 3 cut(s) 99, 291, 1077
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 6 cut(s) 60, 168, 665, 920, 951, 1076
BsmAI GTCTC 3 cut(s) 108, 529, 791
BsmBI CGTCTC 2 cut(s) 108, 791
BsmFI GGGAC 1 cut(s) 226
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 3 cut(s) 30, 259, 279
Bso31I GGTCTC 1 cut(s) 529
Bsp119I TTCGAA 1 cut(s) 751
Bsp1286I GDGCHC 7 cut(s) 84, 126, 195, 546, 570, 867, 968
Bsp143I GATC 9 cut(s) 45, 138, 230, 249, 336, 462, 481, 845, 990
Bsp1720I GCTNAGC 1 cut(s) 650
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 7 cut(s) 36, 119, 168, 329, 587, 637, 1135
BspANI GGCC 3 cut(s) 30, 259, 279
BspCNI CTCAG 6 cut(s) 364, 539, 663, 853, 1282, 1342
BspDI ATCGAT 1 cut(s) 339
BspFNI CGCG 2 cut(s) 119, 329
BspLI GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
BspPI GGATC 1 cut(s) 146
BspT104I TTCGAA 1 cut(s) 751
BspT107I GGYRCC 3 cut(s) 50, 95, 287
BspTNI GGTCTC 1 cut(s) 529
BsrFI RCCGGY 1 cut(s) 1076
BsrI ACTGG 6 cut(s) 31, 162, 280, 305, 433, 1201
BssAI RCCGGY 1 cut(s) 1076
BssECI CCNNGG 6 cut(s) 22, 182, 209, 216, 321, 727
BssMI GATC 9 cut(s) 45, 138, 230, 249, 336, 462, 481, 845, 990
BssNI GRCGYC 2 cut(s) 51, 821
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 5 cut(s) 23, 210, 729, 1253, 1277
Bst4CI ACNGT 4 cut(s) 245, 535, 856, 1057
BstACI GRCGYC 2 cut(s) 51, 821
BstBAI YACGTR 2 cut(s) 178, 827
BstBI TTCGAA 1 cut(s) 751
BstC8I GCNNGC 4 cut(s) 389, 759, 1082, 1148
BstDEI CTNAG 8 cut(s) 372, 519, 526, 650, 861, 969, 1290, 1329
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 7 cut(s) 492, 595, 771, 1065, 1091, 1285, 1303
BstFNI CGCG 2 cut(s) 119, 329
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 4 cut(s) 53, 77, 329, 905
BstKTI GATC 9 cut(s) 48, 141, 233, 252, 339, 465, 484, 848, 993
BstMAI GTCTC 3 cut(s) 108, 529, 791
BstMBI GATC 9 cut(s) 45, 138, 230, 249, 336, 462, 481, 845, 990
BstMWI GCNNNNNNNGC 5 cut(s) 36, 93, 660, 871, 1143
BstNI CCWGG 5 cut(s) 23, 210, 729, 1253, 1277
BstNSI RCATGY 1 cut(s) 714
BstPAI GACNNNNGTC 1 cut(s) 915
BstSCI CCNGG 5 cut(s) 21, 208, 727, 1251, 1275
BstSLI GKGCMC 1 cut(s) 570
BstUI CGCG 2 cut(s) 119, 329
BstV1I GCAGC 1 cut(s) 917
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 3 cut(s) 230, 249, 845
BstYI RGATCY 3 cut(s) 230, 249, 845
Bsu15I ATCGAT 1 cut(s) 339
Bsu36I CCTNAGG 1 cut(s) 519
BsuRI GGCC 3 cut(s) 30, 259, 279
BsuTUI ATCGAT 1 cut(s) 339
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 7 cut(s) 492, 595, 771, 1065, 1091, 1285, 1303
BtsI GCAGTG 1 cut(s) 1155
BtsIMutI CAGTG 4 cut(s) 38, 861, 1155, 1194
Cac8I GCNNGC 4 cut(s) 389, 759, 1082, 1148
CaiI CAGNNNCTG 2 cut(s) 860, 1232
CfoI GCGC 4 cut(s) 53, 77, 329, 905
Cfr10I RCCGGY 1 cut(s) 1076
Cfr13I GGNCC 4 cut(s) 199, 213, 236, 601
ClaI ATCGAT 1 cut(s) 339
CseI GACGC 2 cut(s) 125, 214
CsiI ACCWGGT 1 cut(s) 1251
Csp6I GTAC 6 cut(s) 106, 175, 426, 444, 1192, 1273
CspCI CAANNNNNGTGG 2 cut(s) 1084, 1119
CviQI GTAC 6 cut(s) 106, 175, 426, 444, 1192, 1273
DdeI CTNAG 8 cut(s) 372, 519, 526, 650, 861, 969, 1290, 1329
DinI GGCGCC 1 cut(s) 52
DpnI GATC 9 cut(s) 47, 140, 232, 251, 338, 464, 483, 847, 992
DpnII GATC 9 cut(s) 45, 138, 230, 249, 336, 462, 481, 845, 990
DrdI GACNNNNNNGTC 1 cut(s) 243
DseDI GACNNNNNNGTC 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 277
EciI GGCGGA 1 cut(s) 1124
Ecl136II GAGCTC 2 cut(s) 124, 865
Eco130I CCWWGG 1 cut(s) 216
Eco147I AGGCCT 1 cut(s) 259
Eco24I GRGCYC 3 cut(s) 126, 546, 867
Eco31I GGTCTC 1 cut(s) 529
Eco47I GGWCC 4 cut(s) 199, 213, 236, 601
Eco53kI GAGCTC 2 cut(s) 124, 865
Eco57I CTGAAG 1 cut(s) 238
Eco72I CACGTG 1 cut(s) 827
Eco81I CCTNAGG 1 cut(s) 519
EcoICRI GAGCTC 2 cut(s) 124, 865
EcoRII CCWGG 5 cut(s) 21, 208, 727, 1251, 1275
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 3 cut(s) 126, 546, 867
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 2 cut(s) 108, 791
FalI AAGNNNNNCTT 2 cut(s) 1166, 1198
FaqI GGGAC 1 cut(s) 226
FauI CCCGC 1 cut(s) 161
FbaI TGATCA 1 cut(s) 990
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 2 cut(s) 37, 906
FokI GGATG 7 cut(s) 499, 602, 758, 1052, 1078, 1292, 1310
FriOI GRGCYC 3 cut(s) 126, 546, 867
Fsp4HI GCNGC 2 cut(s) 37, 906
FspBI CTAG 1 cut(s) 416
FspI TGCGCA 1 cut(s) 904
GlaI GCGC 4 cut(s) 52, 76, 328, 904
GluI GCNGC 2 cut(s) 37, 906
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 3 cut(s) 30, 259, 279
HapII CCGG 3 cut(s) 99, 291, 1077
HgaI GACGC 2 cut(s) 125, 214
HhaI GCGC 4 cut(s) 53, 77, 329, 905
Hin1I GRCGYC 2 cut(s) 51, 821
Hin6I GCGC 4 cut(s) 51, 75, 327, 903
HinP1I GCGC 4 cut(s) 51, 75, 327, 903
HincII GTYRAC 1 cut(s) 1053
HindII GTYRAC 1 cut(s) 1053
HindIII AAGCTT 2 cut(s) 661, 755
HinfI GANTC 4 cut(s) 10, 341, 884, 1033
HpaII CCGG 3 cut(s) 99, 291, 1077
HphI GGTGA 1 cut(s) 1211
Hpy166II GTNNAC 7 cut(s) 148, 568, 833, 1053, 1155, 1194, 1224
Hpy188I TCNGA 8 cut(s) 230, 249, 467, 486, 529, 745, 933, 1261
Hpy188III TCNNGA 7 cut(s) 234, 350, 580, 1000, 1124, 1168, 1331
Hpy8I GTNNAC 7 cut(s) 148, 568, 833, 1053, 1155, 1194, 1224
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 7 cut(s) 96, 301, 758, 787, 1108, 1168, 1196
HpyCH4III ACNGT 4 cut(s) 245, 535, 856, 1057
HpyCH4IV ACGT 3 cut(s) 177, 821, 826
HpyCH4V TGCA 6 cut(s) 391, 554, 568, 692, 1084, 1186
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 93, 660, 871, 1143
HpyF3I CTNAG 8 cut(s) 372, 519, 526, 650, 861, 969, 1290, 1329
HpySE526I ACGT 3 cut(s) 177, 821, 826
Hsp92I GRCGYC 2 cut(s) 51, 821
HspAI GCGC 4 cut(s) 51, 75, 327, 903
KasI GGCGCC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 990
Kzo9I GATC 9 cut(s) 45, 138, 230, 249, 336, 462, 481, 845, 990
LmnI GCTCC 3 cut(s) 121, 129, 817
Lsp1109I GCAGC 1 cut(s) 917
LweI GCATC 4 cut(s) 96, 480, 1100, 1270
MabI ACCWGGT 1 cut(s) 1251
MaeI CTAG 1 cut(s) 416
MaeII ACGT 3 cut(s) 177, 821, 826
MaeIII GTNAC 4 cut(s) 311, 706, 822, 1061
MalI GATC 9 cut(s) 47, 140, 232, 251, 338, 464, 483, 847, 992
MboI GATC 9 cut(s) 45, 138, 230, 249, 336, 462, 481, 845, 990
MboII GAAGA 4 cut(s) 5, 244, 499, 787
MflI RGATCY 3 cut(s) 230, 249, 845
MhlI GDGCHC 7 cut(s) 84, 126, 195, 546, 570, 867, 968
MluCI AATT 2 cut(s) 376, 1119
Mly113I GGCGCC 1 cut(s) 51
MroXI GAANNNNTTC 2 cut(s) 1032, 1178
MslI CAYNNNNRTG 1 cut(s) 1160
MspA1I CMGCKG 3 cut(s) 574, 908, 1289
MspI CCGG 3 cut(s) 99, 291, 1077
MspR9I CCNGG 5 cut(s) 23, 210, 729, 1253, 1277
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 5 cut(s) 23, 210, 729, 1253, 1277
MvnI CGCG 2 cut(s) 119, 329
MwoI GCNNNNNNNGC 5 cut(s) 36, 93, 660, 871, 1143
NarI GGCGCC 1 cut(s) 51
NcoI CCATGG 1 cut(s) 216
NdeII GATC 9 cut(s) 45, 138, 230, 249, 336, 462, 481, 845, 990
NlaIV GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
NmeAIII GCCGAG 2 cut(s) 103, 207
NmuCI GTSAC 4 cut(s) 311, 706, 822, 1061
NsbI TGCGCA 1 cut(s) 904
NspI RCATGY 1 cut(s) 714
NspV TTCGAA 1 cut(s) 751
PceI AGGCCT 1 cut(s) 259
PctI GAATGC 1 cut(s) 589
PdmI GAANNNNTTC 2 cut(s) 1032, 1178
PfeI GAWTC 4 cut(s) 10, 341, 884, 1033
PflFI GACNNNGTC 1 cut(s) 224
PflMI CCANNNNNTGG 2 cut(s) 60, 665
PkrI GCNGC 2 cut(s) 38, 907
PluTI GGCGCC 1 cut(s) 54
PmaCI CACGTG 1 cut(s) 827
PmlI CACGTG 1 cut(s) 827
Ppu21I YACGTR 2 cut(s) 178, 827
PshAI GACNNNNGTC 1 cut(s) 915
Psp124BI GAGCTC 2 cut(s) 126, 867
Psp6I CCWGG 5 cut(s) 21, 208, 727, 1251, 1275
PspCI CACGTG 1 cut(s) 827
PspGI CCWGG 5 cut(s) 21, 208, 727, 1251, 1275
PspN4I GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
PspPI GGNCC 4 cut(s) 199, 213, 236, 601
PsrI GAACNNNNNNTAC 2 cut(s) 1001, 1033
PstNI CAGNNNCTG 2 cut(s) 860, 1232
PsuI RGATCY 3 cut(s) 230, 249, 845
PsyI GACNNNGTC 1 cut(s) 224
PvuII CAGCTG 3 cut(s) 574, 908, 1289
RsaI GTAC 6 cut(s) 107, 176, 427, 445, 1193, 1274
RsaNI GTAC 6 cut(s) 106, 175, 426, 444, 1192, 1273
RseI CAYNNNNRTG 1 cut(s) 1160
SacI GAGCTC 2 cut(s) 126, 867
SatI GCNGC 2 cut(s) 37, 906
Sau3AI GATC 9 cut(s) 45, 138, 230, 249, 336, 462, 481, 845, 990
Sau96I GGNCC 4 cut(s) 199, 213, 236, 601
ScrFI CCNGG 5 cut(s) 23, 210, 729, 1253, 1277
SduI GDGCHC 7 cut(s) 84, 126, 195, 546, 570, 867, 968
SexAI ACCWGGT 1 cut(s) 1251
SfaNI GCATC 4 cut(s) 96, 480, 1100, 1270
SfoI GGCGCC 1 cut(s) 52
SfuI TTCGAA 1 cut(s) 751
SinI GGWCC 4 cut(s) 199, 213, 236, 601
SmiMI CAYNNNNRTG 1 cut(s) 1160
SmlI CTYRAG 3 cut(s) 131, 203, 644
SmoI CTYRAG 3 cut(s) 131, 203, 644
Sse9I AATT 2 cut(s) 376, 1119
SseBI AGGCCT 1 cut(s) 259
SsiI CCGC 7 cut(s) 36, 119, 168, 329, 587, 637, 1135
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 1 cut(s) 416
SstI GAGCTC 2 cut(s) 126, 867
StuI AGGCCT 1 cut(s) 259
StyD4I CCNGG 5 cut(s) 21, 208, 727, 1251, 1275
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 4 cut(s) 245, 535, 856, 1057
TaiI ACGT 3 cut(s) 180, 824, 829
TaqI TCGA 6 cut(s) 90, 339, 351, 579, 751, 1112
TaqII GACCGA 1 cut(s) 493
TasI AATT 2 cut(s) 376, 1119
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 4 cut(s) 10, 341, 884, 1033
TscAI CASTG 4 cut(s) 38, 861, 1162, 1201
TseFI GTSAC 4 cut(s) 311, 706, 822, 1061
TseI GCWGC 1 cut(s) 905
Tsp45I GTSAC 4 cut(s) 311, 706, 822, 1061
TspDTI ATGAA 6 cut(s) 5, 787, 996, 1090, 1259, 1314
TspRI CASTG 4 cut(s) 38, 861, 1162, 1201
Tth111I GACNNNGTC 1 cut(s) 224
Van91I CCANNNNNTGG 2 cut(s) 60, 665
VneI GTGCAC 1 cut(s) 566
VpaK11BI GGWCC 4 cut(s) 199, 213, 236, 601
XceI RCATGY 1 cut(s) 714
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 2 cut(s) 1032, 1178
XspI CTAG 1 cut(s) 416
ZraI GACGTC 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.