FvH4_6g15720

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007518
Physical Location & Seq
Reverse (-)
9803518 .. 9807979
4462 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g15720.t4

Sequence Viewer

Length: 1521 bp
ATGGCAATTGAAGAAGAAGAAGCTTCTGCTAAGCAAAATGATGCTGCTGACATTTCCACCAGTCAAGTTGATCCTTCACCCCAAGAGGATGGAAATTCCCCTGAGATAATTGAAGAAACTCCTCCTGAACACCCATGGAGACGACAGAACCTCGCCTTACAGATACCCTCAAGAACTCTTGCGGATGCCAAAGAGGATTTTGTGAGAATAGACATGCCCCCAACTCCAAGTCCCACTCCCAAAAGAGTAAACTTCTCCCCATTACCCAGCCCTTATTTAAACAAAGTCAGTGGATCCCCAGGTCCCTCATCCTCAAAAACTAAGACAACCATAAAAAGCCTCCTTCCCAAACTAAGTTTCAAGTATCGGAACACTACTTCAGAGATACGGAAGGCTGCTACGCTTGCACTAGGAGGTACACCTACGGCAACCCGTCAGAGGCCTATAATTTCTAGGACATTTTCTCTTACAAACCTTTTAACACCAAGTCTGAAGAATACATCATCATTGCCTACAACTCCAATTTCTCACTCAAATCCAGAGTCTATGCATGGGAGGAACACTGTTGATCTGAAAGGAGGGCGCCAGCTGCCTATTCATCGTTCACATTCAGTACCTGTGCTTAATAAAGATGGGAGTGTATGTCTAGGTGGTGTCATTCGAGTGATTCCTTCCACACCACGAGCGATGGAGAGAACTGTTTCAATAACTTCATGCACCTCCCCAAAAGATGACAATGATGGAAGTGATGATGATGGTGAAGATATTGCCGCTGAAGAGGCTGTTTGTCGAATTTGCTTAGTTGAACTGGGGGAAGGTGCTGACACCCTGAAGATGGAATGCAGCTGTAAAGGCGACCTCGCTCTGGCCCACCAAGAGTGTGCTGTAAAATGGTTCAGCATTAAAGGTAATAAAACATGTGATGTGTGCAAGCAAGAGGTCCAGAACCTGCCTGTCACGCTTTTACGAATTCAAAATGTTCAAGCACATAATTTACGTGGAACTAGAGCACAGCAGCCTGAGGTTACTCAATACAGGGTCTGGCAGGATGTTCCCATTCTCGTCATAGTTAGCATGCTCGCATACTTCTGTTTTCTTGAGCAGCTTCTGGTAGGAAAAATGGGATCAGGTGCAATTGCGCTCTCTCTTCCTTTTTCCTGTATATTGGGTCTTTTGGCATCCATGACAGCAAGCACAATGGTGAGAAGAAAATATGTCTGGGTTTATGCGACCATTCAGTTCTTTTTGGTGGTTCTCTCAGCTCATCTACTTTATTCATTGATTCACATGCAGGCAGTTCTGTCGGTTCTTCTTGCCACATTCACGGGGTTTGGAGTTACAATGTGTGGGAATTCCATTATTATCGAGGCTTTGAGATGGAGGGAAAGATGTCTTTCTCGGTCAAATCAACGTCATGGTACTGCTGCAGTGACTCAGCCGAATCAACCACAACAAACAGAGACAAATCCTCAGCATCTTGAAAATGAAATTGAAGGTGCTGAAGCTGTTCGTGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

507

Amino Acids

55.35

Weight (kDa)

7.55

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 263 - 310 5.9e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 957
AccB1I GGYRCC 1 cut(s) 582
AciI CCGC 2 cut(s) 182, 771
AclWI GGATC 4 cut(s) 65, 288, 301, 1132
AcsI RAATTY 4 cut(s) 94, 792, 969, 1351
AcuI CTGAAG 5 cut(s) 363, 512, 795, 851, 1521
AcyI GRCGYC 1 cut(s) 583
AfaI GTAC 3 cut(s) 418, 615, 1420
AfiI CCNNNNNNNGG 3 cut(s) 438, 835, 865
AflIII ACRYGT 1 cut(s) 917
AgsI TTSAA 9 cut(s) 11, 113, 361, 705, 806, 974, 983, 1481, 1493
AjnI CCWGG 1 cut(s) 298
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AleI CACNNNNGTG 1 cut(s) 1199
AluBI AGCT 7 cut(s) 23, 589, 846, 1105, 1262, 1505, 1518
AluI AGCT 7 cut(s) 23, 589, 846, 1105, 1262, 1505, 1518
Alw21I GWGCWC 1 cut(s) 1012
Alw26I GTCTC 2 cut(s) 133, 1454
AlwI GGATC 4 cut(s) 65, 288, 301, 1132
AlwNI CAGNNNCTG 4 cut(s) 617, 949, 1041, 1108
AoxI GGCC 2 cut(s) 440, 867
ApeKI GCWGC 8 cut(s) 44, 395, 589, 843, 1015, 1102, 1424, 1515
ApoI RAATTY 4 cut(s) 94, 792, 969, 1351
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp700I GAANNNNTTC 2 cut(s) 700, 1506
AspLEI GCGC 2 cut(s) 585, 1141
AspS9I GGNCC 3 cut(s) 302, 868, 940
AsuHPI GGTGA 3 cut(s) 69, 770, 1213
AvaII GGWCC 2 cut(s) 302, 940
AxyI CCTNAGG 1 cut(s) 1020
BamHI GGATCC 1 cut(s) 293
BanI GGYRCC 1 cut(s) 582
BauI CACGAG 1 cut(s) 681
Bbv12I GWGCWC 1 cut(s) 1012
BbvCI CCTCAGC 1 cut(s) 1470
BbvI GCAGC 7 cut(s) 31, 382, 576, 855, 1027, 1114, 1411
BccI CCATC 7 cut(s) 83, 626, 682, 734, 749, 829, 1371
BceAI ACGGC 1 cut(s) 441
BcgI CGANNNNNNTGC 2 cut(s) 1284, 1318
BciT130I CCWGG 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 133, 1454
BfaI CTAG 4 cut(s) 410, 453, 647, 1005
BfmI CTRYAG 1 cut(s) 1425
BfoI RGCGCY 1 cut(s) 586
BfuAI ACCTGC 1 cut(s) 957
BisI GCNGC 9 cut(s) 45, 396, 590, 771, 844, 1016, 1103, 1425, 1516
BlpI GCTNAGC 1 cut(s) 30
BlsI GCNGC 9 cut(s) 46, 397, 591, 772, 845, 1017, 1104, 1426, 1517
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 2 cut(s) 302, 940
BmgT120I GGNCC 3 cut(s) 302, 868, 940
BmiI GGNNCC 3 cut(s) 295, 304, 584
BmrFI CCNGG 1 cut(s) 300
BmrI ACTGGG 1 cut(s) 818
BmsI GCATC 4 cut(s) 31, 175, 1187, 1483
BmuI ACTGGG 1 cut(s) 818
Bpu10I CCTNAGC 1 cut(s) 1470
Bpu1102I GCTNAGC 1 cut(s) 30
BpuEI CTTGAG 2 cut(s) 154, 1118
BsaAI YACGTR 1 cut(s) 998
BsaHI GRCGYC 1 cut(s) 583
BsaJI CCNNGG 2 cut(s) 134, 298
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 3 cut(s) 438, 835, 865
Bse1I ACTGG 2 cut(s) 60, 813
Bse21I CCTNAGG 1 cut(s) 1020
Bse3DI GCAATG 1 cut(s) 506
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 134, 298
BseGI GGATG 5 cut(s) 94, 190, 308, 1054, 1178
BseLI CCNNNNNNNGG 3 cut(s) 438, 835, 865
BseMI GCAATG 1 cut(s) 506
BseMII CTCAG 5 cut(s) 93, 1011, 1272, 1448, 1484
BseNI ACTGG 2 cut(s) 60, 813
BseRI GAGGAG 1 cut(s) 111
BseXI GCAGC 7 cut(s) 31, 382, 576, 855, 1027, 1114, 1411
BseYI CCCAGC 1 cut(s) 266
BshFI GGCC 2 cut(s) 442, 869
BshNI GGYRCC 1 cut(s) 582
BsiHKAI GWGCWC 1 cut(s) 1012
BslFI GGGAC 2 cut(s) 216, 288
BslI CCNNNNNNNGG 3 cut(s) 438, 835, 865
BsmAI GTCTC 2 cut(s) 133, 1454
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 216, 288
BsmI GAATGC 1 cut(s) 845
BsnI GGCC 2 cut(s) 442, 869
Bsp1286I GDGCHC 1 cut(s) 1012
Bsp143I GATC 4 cut(s) 70, 293, 568, 1124
Bsp1720I GCTNAGC 1 cut(s) 30
Bsp19I CCATGG 1 cut(s) 134
BspACI CCGC 2 cut(s) 182, 771
BspANI GGCC 2 cut(s) 442, 869
BspCNI CTCAG 5 cut(s) 94, 1012, 1271, 1447, 1483
BspLI GGNNCC 3 cut(s) 295, 304, 584
BspMAI CTGCAG 1 cut(s) 1429
BspMI ACCTGC 1 cut(s) 957
BspPI GGATC 4 cut(s) 65, 288, 301, 1132
BspT107I GGYRCC 1 cut(s) 582
BsrDI GCAATG 1 cut(s) 506
BsrI ACTGG 2 cut(s) 60, 813
BssECI CCNNGG 2 cut(s) 134, 298
BssMI GATC 4 cut(s) 70, 293, 568, 1124
BssNI GRCGYC 1 cut(s) 583
BssSI CACGAG 1 cut(s) 681
BssT1I CCWWGG 1 cut(s) 134
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 2 cut(s) 565, 700
Bst6I CTCTTC 2 cut(s) 771, 1152
BstACI GRCGYC 1 cut(s) 583
BstBAI YACGTR 1 cut(s) 998
BstC8I GCNNGC 7 cut(s) 405, 587, 932, 1076, 1080, 1192, 1293
BstDEI CTNAG 9 cut(s) 30, 102, 321, 353, 799, 1020, 1258, 1434, 1470
BstDSI CCRYGG 1 cut(s) 134
BstF5I GGATG 5 cut(s) 94, 190, 308, 1054, 1178
BstH2I RGCGCY 1 cut(s) 586
BstHHI GCGC 2 cut(s) 585, 1141
BstKTI GATC 4 cut(s) 73, 296, 571, 1127
BstMAI GTCTC 2 cut(s) 133, 1454
BstMBI GATC 4 cut(s) 70, 293, 568, 1124
BstMWI GCNNNNNNNGC 5 cut(s) 404, 589, 779, 852, 958
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 4 cut(s) 217, 921, 1078, 1291
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 1425
BstV1I GCAGC 7 cut(s) 31, 382, 576, 855, 1027, 1114, 1411
BstX2I RGATCY 1 cut(s) 293
BstXI CCANNNNNNTGG 1 cut(s) 89
BstYI RGATCY 1 cut(s) 293
Bsu36I CCTNAGG 1 cut(s) 1020
BsuRI GGCC 2 cut(s) 442, 869
BtgI CCRYGG 1 cut(s) 134
BtgZI GCGATG 1 cut(s) 701
BtsCI GGATG 5 cut(s) 94, 190, 308, 1054, 1178
BtsI GCAGTG 1 cut(s) 1434
BtsIMutI CAGTG 3 cut(s) 295, 561, 1434
BveI ACCTGC 1 cut(s) 957
Cac8I GCNNGC 7 cut(s) 405, 587, 932, 1076, 1080, 1192, 1293
CaiI CAGNNNCTG 4 cut(s) 617, 949, 1041, 1108
CfoI GCGC 2 cut(s) 585, 1141
Cfr13I GGNCC 3 cut(s) 302, 868, 940
Csp6I GTAC 3 cut(s) 417, 614, 1419
CspCI CAANNNNNGTGG 2 cut(s) 271, 306
CviAII CATG 9 cut(s) 135, 214, 551, 714, 918, 1075, 1183, 1288, 1415
CviQI GTAC 3 cut(s) 417, 614, 1419
DdeI CTNAG 9 cut(s) 30, 102, 321, 353, 799, 1020, 1258, 1434, 1470
DinI GGCGCC 1 cut(s) 584
DpnI GATC 4 cut(s) 72, 295, 570, 1126
DpnII GATC 4 cut(s) 70, 293, 568, 1124
DraI TTTAAA 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 771, 1152
EarI CTCTTC 2 cut(s) 771, 1152
Eco130I CCWWGG 1 cut(s) 134
Eco147I AGGCCT 1 cut(s) 442
Eco47I GGWCC 2 cut(s) 302, 940
Eco57I CTGAAG 5 cut(s) 363, 512, 795, 851, 1521
Eco81I CCTNAGG 1 cut(s) 1020
EcoO109I RGGNCCY 1 cut(s) 302
EcoRI GAATTC 2 cut(s) 969, 1351
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 134
EcoT22I ATGCAT 1 cut(s) 552
EgeI GGCGCC 1 cut(s) 584
EheI GGCGCC 1 cut(s) 584
ErhI CCWWGG 1 cut(s) 134
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 9 cut(s) 138, 217, 554, 717, 921, 1078, 1186, 1291, 1418
FaqI GGGAC 2 cut(s) 216, 288
FatI CATG 9 cut(s) 134, 213, 550, 713, 917, 1074, 1182, 1287, 1414
Fnu4HI GCNGC 9 cut(s) 45, 396, 590, 771, 844, 1016, 1103, 1425, 1516
FokI GGATG 5 cut(s) 101, 197, 295, 1061, 1165
Fsp4HI GCNGC 9 cut(s) 45, 396, 590, 771, 844, 1016, 1103, 1425, 1516
FspBI CTAG 4 cut(s) 410, 453, 647, 1005
GlaI GCGC 2 cut(s) 584, 1140
GluI GCNGC 9 cut(s) 45, 396, 590, 771, 844, 1016, 1103, 1425, 1516
GsaI CCCAGC 1 cut(s) 270
HaeII RGCGCY 1 cut(s) 586
HaeIII GGCC 2 cut(s) 442, 869
HhaI GCGC 2 cut(s) 585, 1141
Hin1I GRCGYC 1 cut(s) 583
Hin1II CATG 9 cut(s) 138, 217, 554, 717, 921, 1078, 1186, 1291, 1418
Hin6I GCGC 2 cut(s) 583, 1139
HinP1I GCGC 2 cut(s) 583, 1139
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 5 cut(s) 542, 667, 1282, 1432, 1441
HphI GGTGA 3 cut(s) 69, 770, 1213
Hpy166II GTNNAC 3 cut(s) 250, 419, 605
Hpy188I TCNGA 5 cut(s) 369, 382, 438, 492, 573
Hpy188III TCNNGA 6 cut(s) 125, 171, 539, 943, 1097, 1478
Hpy8I GTNNAC 3 cut(s) 250, 419, 605
HpyAV CCTTC 6 cut(s) 84, 353, 385, 681, 809, 1487
HpyCH4III ACNGT 2 cut(s) 565, 700
HpyCH4IV ACGT 2 cut(s) 997, 1411
HpyCH4V TGCA 8 cut(s) 407, 550, 717, 843, 930, 1133, 1291, 1427
HpyF10VI GCNNNNNNNGC 5 cut(s) 404, 589, 779, 852, 958
HpyF3I CTNAG 9 cut(s) 30, 102, 321, 353, 799, 1020, 1258, 1434, 1470
HpySE526I ACGT 2 cut(s) 997, 1411
Hsp92I GRCGYC 1 cut(s) 583
Hsp92II CATG 9 cut(s) 138, 217, 554, 717, 921, 1078, 1186, 1291, 1418
HspAI GCGC 2 cut(s) 583, 1139
KasI GGCGCC 1 cut(s) 582
Kzo9I GATC 4 cut(s) 70, 293, 568, 1124
Lsp1109I GCAGC 7 cut(s) 31, 382, 576, 855, 1027, 1114, 1411
LweI GCATC 4 cut(s) 31, 175, 1187, 1483
MaeI CTAG 4 cut(s) 410, 453, 647, 1005
MaeII ACGT 2 cut(s) 997, 1411
MaeIII GTNAC 4 cut(s) 955, 1024, 1336, 1429
MalI GATC 4 cut(s) 72, 295, 570, 1126
MboI GATC 4 cut(s) 70, 293, 568, 1124
MfeI CAATTG 2 cut(s) 6, 1134
MflI RGATCY 1 cut(s) 293
MhlI GDGCHC 1 cut(s) 1012
Mly113I GGCGCC 1 cut(s) 583
MlyI GAGTC 2 cut(s) 551, 1426
Mph1103I ATGCAT 1 cut(s) 552
MroXI GAANNNNTTC 2 cut(s) 700, 1506
MseI TTAA 4 cut(s) 278, 479, 624, 903
MslI CAYNNNNRTG 2 cut(s) 662, 1199
MspA1I CMGCKG 4 cut(s) 589, 773, 846, 1518
MspR9I CCNGG 1 cut(s) 300
MunI CAATTG 2 cut(s) 6, 1134
Mva1269I GAATGC 1 cut(s) 845
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 5 cut(s) 404, 589, 779, 852, 958
NarI GGCGCC 1 cut(s) 583
NcoI CCATGG 1 cut(s) 134
NdeII GATC 4 cut(s) 70, 293, 568, 1124
NlaIII CATG 9 cut(s) 138, 217, 554, 717, 921, 1078, 1186, 1291, 1418
NlaIV GGNNCC 3 cut(s) 295, 304, 584
NmuCI GTSAC 2 cut(s) 955, 1429
NsiI ATGCAT 1 cut(s) 552
NspI RCATGY 4 cut(s) 217, 921, 1078, 1291
OliI CACNNNNGTG 1 cut(s) 1199
PaeI GCATGC 1 cut(s) 1078
PceI AGGCCT 1 cut(s) 442
PciI ACATGT 1 cut(s) 917
PctI GAATGC 1 cut(s) 845
PdmI GAANNNNTTC 2 cut(s) 700, 1506
PfeI GAWTC 3 cut(s) 667, 1282, 1441
PkrI GCNGC 9 cut(s) 46, 397, 591, 772, 845, 1017, 1104, 1426, 1517
PleI GAGTC 2 cut(s) 550, 1426
PluTI GGCGCC 1 cut(s) 586
PpsI GAGTC 2 cut(s) 550, 1426
Ppu21I YACGTR 1 cut(s) 998
PpuMI RGGWCCY 1 cut(s) 302
PscI ACATGT 1 cut(s) 917
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 266
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 3 cut(s) 295, 304, 584
PspPI GGNCC 3 cut(s) 302, 868, 940
PspPPI RGGWCCY 1 cut(s) 302
PstI CTGCAG 1 cut(s) 1429
PstNI CAGNNNCTG 4 cut(s) 617, 949, 1041, 1108
PsuI RGATCY 1 cut(s) 293
PvuII CAGCTG 3 cut(s) 589, 846, 1518
RsaI GTAC 3 cut(s) 418, 615, 1420
RsaNI GTAC 3 cut(s) 417, 614, 1419
RseI CAYNNNNRTG 2 cut(s) 662, 1199
SaqAI TTAA 4 cut(s) 278, 479, 624, 903
SatI GCNGC 9 cut(s) 45, 396, 590, 771, 844, 1016, 1103, 1425, 1516
Sau3AI GATC 4 cut(s) 70, 293, 568, 1124
Sau96I GGNCC 3 cut(s) 302, 868, 940
SchI GAGTC 2 cut(s) 551, 1426
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 1 cut(s) 1012
SfaNI GCATC 4 cut(s) 31, 175, 1187, 1483
SfcI CTRYAG 1 cut(s) 1425
SfoI GGCGCC 1 cut(s) 584
SinI GGWCC 2 cut(s) 302, 940
SmiMI CAYNNNNRTG 2 cut(s) 662, 1199
SmlI CTYRAG 2 cut(s) 169, 1097
SmoI CTYRAG 2 cut(s) 169, 1097
SphI GCATGC 1 cut(s) 1078
SseBI AGGCCT 1 cut(s) 442
SsiI CCGC 2 cut(s) 182, 771
SspDI GGCGCC 1 cut(s) 582
SspMI CTAG 4 cut(s) 410, 453, 647, 1005
StuI AGGCCT 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 1 cut(s) 134
TaaI ACNGT 2 cut(s) 565, 700
TaiI ACGT 2 cut(s) 1000, 1414
TaqI TCGA 3 cut(s) 661, 790, 1365
TaqII GACCGA 1 cut(s) 1389
TauI GCSGC 1 cut(s) 773
TfiI GAWTC 3 cut(s) 667, 1282, 1441
Tru1I TTAA 4 cut(s) 278, 479, 624, 903
Tru9I TTAA 4 cut(s) 278, 479, 624, 903
TscAI CASTG 3 cut(s) 295, 568, 1434
TseFI GTSAC 2 cut(s) 955, 1429
TseI GCWGC 8 cut(s) 44, 395, 589, 843, 1015, 1102, 1424, 1515
Tsp45I GTSAC 2 cut(s) 955, 1429
TspDTI ATGAA 4 cut(s) 587, 702, 1266, 1500
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 3 cut(s) 295, 568, 1434
VpaK11BI GGWCC 2 cut(s) 302, 940
XapI RAATTY 4 cut(s) 94, 792, 969, 1351
XceI RCATGY 4 cut(s) 217, 921, 1078, 1291
XmnI GAANNNNTTC 2 cut(s) 700, 1506
XspI CTAG 4 cut(s) 410, 453, 647, 1005
Zsp2I ATGCAT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.