pycom04g09490

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007518
Physical Location & Seq
Forward (+)
12119535 .. 12123109
3575 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g09490.1

Sequence Viewer

Length: 1506 bp
ATGGCGACTGAAGATGAAAGCATCGTTAAAAATGGTGGCAACATTTTCACCAACGAAGCTGAGCCTTCAGCCCACATGGGTGGAGATTCCATTGAGATAATTGAAGAAGCTCCTCCTGACCATCCATGGAGACGACAGAACCTTGTGTTACAGATACCTCCAAGAACTCTTGAGGATGCCAAAGAAGATTTTGTAAGCATAAACATGCCCCCAACGCCGAGTCCCACTCCCAAAAGAGTAAACTTCTCCCCATTACCCAGCCCTATTGTAGCCAAAATCAGTGGTTCCCCAGGTCCCTCATCATCAAAAACTAAATCAACTACAAAAAAAAGCCTCCTTCCAAAACTAAGTTTCAAACATCGGAACACTACTTCGGAGATTCAGAAGGCCGCCGCTCTAGCACTAGGAGGCTTACCTGCAGGGACACGTGAGAAGCCTACAATTCCAAGGGCCTGGTCCTTTACAAAGCTTTTAACTCCCAGAACGAAAAATACATCATCCTTGCCTGTAACTCCAATTGCTCACTCAAATCCAGAGTCTATGCATGGCAGGAACACAACTGATCTGAAAGCAGAGGCCCAACGACCTATTCACCGTTCACTTTCAGTCCCTGCAATTAATAAAGATGGAAGTATATGTGTAGGCAGTGTCATTCGTGTAATTCCAACCACGCCACAAGTGGCTGAAGGGACTGTCACGACAACATCAAGCACATCCCCTGTAAATGACACTGGTGGAAGCAATGATCGTGGTGAAGATATTGCTGAAGAAGAAGCTGTTTGTCGAATTTGCTTAGTTGAGCTGGGTGAAGACGCTGACACCCTCAAGATGGAATGCAGATGTAAAGGCGACCTTGCTCTTGCCCACCAAGAATGTGCTGTAAAATGGTTCAGCATTAAAGGTAATAAAAAATGTGATGTGTGCGGAGAAGAGGTTCAGAACCTACCGGTCACCCTTCTACGAATTCAAAATTTTCAGGCTGTTAATTTTCGAGCAAGTAGAGCACAGCAGGCTGAGGCTACTCAATATAGGGTTTGGCAGGATGTTCCCATCCTTGTCATAGTCAGCATGCTTGCTTACTTCTGTTTTCTTGAGCAGCTTCTGGTGGGGAAAATGGGATCGGGTGCAATTGCTCTTTCTCTTCCTTTTTCCTGCATTTTGGGTCTTCTGGCATCCATGACATCGAGCACGATGGTGAGAAGAAGATATGTCTGGATTTATGCAACTATTCAGTTCGTACTGGTGGTTCTCGCGTCACATCTACTTTATTCATTGATTCACATGCAGGCAGTTCTGGCTCTTCTCCTCGCCACCTTTTCAGGATTTGGAGTTACAATGTGCGGAAATTCTATTATTGTCAAGGCTTTGGGATGGAGGGAAAGATGGCGTTCTCAGGAGTCAAATCAACAGCGTGGTTCTCAGGAGGTGGCGCAGCCAAATCAATCACCCCAGAATACACGACATACACAGACGGATCCCCAACAGCGGGAAAATGAACTCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

502

Amino Acids

54.83

Weight (kDa)

8.43

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 261 - 308 2.3e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 424
AccBSI CCGCTC 1 cut(s) 395
AccII CGCG 1 cut(s) 1253
AciI CCGC 5 cut(s) 390, 393, 924, 1341, 1486
AclWI GGATC 3 cut(s) 1126, 1469, 1482
AcsI RAATTY 4 cut(s) 786, 963, 970, 1345
AcuI CTGAAG 4 cut(s) 30, 51, 705, 786
AcvI CACGTG 1 cut(s) 428
AfaI GTAC 1 cut(s) 1239
AfiI CCNNNNNNNGG 3 cut(s) 829, 1485, 1486
AflIII ACRYGT 1 cut(s) 425
AgeI ACCGGT 1 cut(s) 946
AgsI TTSAA 3 cut(s) 104, 355, 968
AjnI CCWGG 2 cut(s) 289, 452
AjuI GAANNNNNNNTTGG 2 cut(s) 573, 605
AleI CACNNNNGTG 2 cut(s) 78, 1193
AloI GAACNNNNNNTCC 2 cut(s) 918, 950
AluBI AGCT 6 cut(s) 59, 110, 469, 776, 802, 1099
AluI AGCT 6 cut(s) 59, 110, 469, 776, 802, 1099
Alw21I GWGCWC 2 cut(s) 1006, 1190
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 3 cut(s) 1126, 1469, 1482
AlwNI CAGNNNCTG 2 cut(s) 611, 1102
AoxI GGCC 3 cut(s) 387, 450, 576
ApeKI GCWGC 2 cut(s) 1096, 1432
ApoI RAATTY 4 cut(s) 786, 963, 970, 1345
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
AseI ATTAAT 1 cut(s) 618
AsiGI ACCGGT 1 cut(s) 946
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 1 cut(s) 1432
AspS9I GGNCC 4 cut(s) 293, 450, 456, 577
AsuHPI GGTGA 7 cut(s) 40, 584, 764, 818, 943, 1207, 1437
AvaII GGWCC 2 cut(s) 293, 456
BamHI GGATCC 1 cut(s) 1474
BbrPI CACGTG 1 cut(s) 428
BbsI GAAGAC 2 cut(s) 816, 1157
Bbv12I GWGCWC 2 cut(s) 1006, 1190
BbvCI CCTCAGC 1 cut(s) 1014
BbvI GCAGC 2 cut(s) 1108, 1444
BccI CCATC 7 cut(s) 129, 620, 823, 1058, 1186, 1365, 1377
BciT130I CCWGG 2 cut(s) 291, 454
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 2 cut(s) 398, 404
BfmI CTRYAG 1 cut(s) 417
BfuAI ACCTGC 1 cut(s) 424
BisI GCNGC 4 cut(s) 390, 393, 1097, 1433
BlpI GCTNAGC 1 cut(s) 60
BlsI GCNGC 4 cut(s) 391, 394, 1098, 1434
Bme1390I CCNGG 2 cut(s) 291, 454
Bme18I GGWCC 2 cut(s) 293, 456
BmgT120I GGNCC 4 cut(s) 293, 450, 456, 577
BmiI GGNNCC 3 cut(s) 286, 295, 1476
BmrFI CCNGG 2 cut(s) 291, 454
BmsI GCATC 3 cut(s) 30, 166, 1181
BpiI GAAGAC 2 cut(s) 816, 1157
BplI GAGNNNNNCTC 2 cut(s) 211, 243
Bpu10I CCTNAGC 1 cut(s) 1014
Bpu1102I GCTNAGC 1 cut(s) 60
BpuEI CTTGAG 3 cut(s) 191, 809, 1112
BsaAI YACGTR 1 cut(s) 428
BsaJI CCNNGG 3 cut(s) 125, 289, 446
BsaWI WCCGGW 1 cut(s) 946
BsaXI ACNNNNNCTCC 2 cut(s) 918, 948
Bsc4I CCNNNNNNNGG 3 cut(s) 829, 1485, 1486
Bse118I RCCGGY 1 cut(s) 946
Bse1I ACTGG 2 cut(s) 736, 1245
Bse3DI GCAATG 1 cut(s) 748
BseBI CCWGG 2 cut(s) 291, 454
BseDI CCNNGG 3 cut(s) 125, 289, 446
BseGI GGATG 8 cut(s) 121, 181, 497, 713, 1048, 1050, 1172, 1376
BseLI CCNNNNNNNGG 3 cut(s) 829, 1485, 1486
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 4 cut(s) 51, 1005, 1406, 1433
BseNI ACTGG 2 cut(s) 736, 1245
BseRI GAGGAG 2 cut(s) 102, 1295
BseXI GCAGC 2 cut(s) 1108, 1444
BseYI CCCAGC 2 cut(s) 257, 802
Bsh1236I CGCG 1 cut(s) 1253
BshFI GGCC 3 cut(s) 389, 452, 578
BshTI ACCGGT 1 cut(s) 946
BsiHKAI GWGCWC 2 cut(s) 1006, 1190
BsiSI CCGG 1 cut(s) 947
BslFI GGGAC 5 cut(s) 207, 279, 436, 593, 703
BslI CCNNNNNNNGG 3 cut(s) 829, 1485, 1486
BsmAI GTCTC 1 cut(s) 124
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 5 cut(s) 207, 279, 436, 593, 703
BsmI GAATGC 1 cut(s) 839
BsnI GGCC 3 cut(s) 389, 452, 578
Bsp1286I GDGCHC 2 cut(s) 1006, 1190
Bsp143I GATC 4 cut(s) 562, 745, 1118, 1474
Bsp1720I GCTNAGC 1 cut(s) 60
Bsp19I CCATGG 1 cut(s) 125
BspACI CCGC 5 cut(s) 390, 393, 924, 1341, 1486
BspANI GGCC 3 cut(s) 389, 452, 578
BspCNI CTCAG 4 cut(s) 52, 1006, 1405, 1432
BspFNI CGCG 1 cut(s) 1253
BspLI GGNNCC 3 cut(s) 286, 295, 1476
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 424
BspPI GGATC 3 cut(s) 1126, 1469, 1482
BspQI GCTCTTC 1 cut(s) 1305
BsrBI CCGCTC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 748
BsrFI RCCGGY 1 cut(s) 946
BsrI ACTGG 2 cut(s) 736, 1245
BssAI RCCGGY 1 cut(s) 946
BssECI CCNNGG 3 cut(s) 125, 289, 446
BssMI GATC 4 cut(s) 562, 745, 1118, 1474
BssT1I CCWWGG 2 cut(s) 125, 446
Bst2UI CCWGG 2 cut(s) 291, 454
Bst4CI ACNGT 2 cut(s) 596, 694
Bst6I CTCTTC 3 cut(s) 924, 1146, 1305
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 4 cut(s) 1011, 1070, 1074, 1287
BstDEI CTNAG 6 cut(s) 60, 347, 793, 1014, 1392, 1419
BstDSI CCRYGG 1 cut(s) 125
BstEII GGTNACC 1 cut(s) 949
BstF5I GGATG 8 cut(s) 121, 181, 497, 713, 1048, 1050, 1172, 1376
BstFNI CGCG 1 cut(s) 1253
BstHHI GCGC 1 cut(s) 1432
BstKTI GATC 4 cut(s) 565, 748, 1121, 1477
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 4 cut(s) 562, 745, 1118, 1474
BstMWI GCNNNNNNNGC 5 cut(s) 214, 398, 1001, 1010, 1295
BstNI CCWGG 2 cut(s) 291, 454
BstNSI RCATGY 3 cut(s) 208, 1072, 1285
BstPI GGTNACC 1 cut(s) 949
BstSCI CCNGG 2 cut(s) 289, 452
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 1253
BstV1I GCAGC 2 cut(s) 1108, 1444
BstV2I GAAGAC 2 cut(s) 816, 1157
BstX2I RGATCY 1 cut(s) 1474
BstXI CCANNNNNNTGG 2 cut(s) 80, 453
BstYI RGATCY 1 cut(s) 1474
BsuRI GGCC 3 cut(s) 389, 452, 578
BtgI CCRYGG 1 cut(s) 125
BtsCI GGATG 8 cut(s) 121, 181, 497, 713, 1048, 1050, 1172, 1376
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 3 cut(s) 286, 652, 729
BveI ACCTGC 1 cut(s) 424
Cac8I GCNNGC 4 cut(s) 1011, 1070, 1074, 1287
CaiI CAGNNNCTG 2 cut(s) 611, 1102
CfoI GCGC 1 cut(s) 1432
Cfr10I RCCGGY 1 cut(s) 946
Cfr13I GGNCC 4 cut(s) 293, 450, 456, 577
CseI GACGC 2 cut(s) 821, 1242
Csp6I GTAC 1 cut(s) 1238
CspAI ACCGGT 1 cut(s) 946
CspCI CAANNNNNGTGG 4 cut(s) 262, 297, 730, 765
CviAII CATG 7 cut(s) 76, 126, 205, 545, 1069, 1177, 1282
CviQI GTAC 1 cut(s) 1238
DdeI CTNAG 6 cut(s) 60, 347, 793, 1014, 1392, 1419
DpnI GATC 4 cut(s) 564, 747, 1120, 1476
DpnII GATC 4 cut(s) 562, 745, 1118, 1474
Eam1104I CTCTTC 3 cut(s) 924, 1146, 1305
EarI CTCTTC 3 cut(s) 924, 1146, 1305
Eco130I CCWWGG 2 cut(s) 125, 446
Eco47I GGWCC 2 cut(s) 293, 456
Eco57I CTGAAG 4 cut(s) 30, 51, 705, 786
Eco72I CACGTG 1 cut(s) 428
Eco91I GGTNACC 1 cut(s) 949
EcoO109I RGGNCCY 2 cut(s) 293, 450
EcoO65I GGTNACC 1 cut(s) 949
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 2 cut(s) 289, 452
EcoT14I CCWWGG 2 cut(s) 125, 446
EcoT22I ATGCAT 1 cut(s) 546
ErhI CCWWGG 2 cut(s) 125, 446
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 7 cut(s) 79, 129, 208, 548, 1072, 1180, 1285
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 5 cut(s) 207, 279, 436, 593, 703
FatI CATG 7 cut(s) 75, 125, 204, 544, 1068, 1176, 1281
FauI CCCGC 1 cut(s) 1479
Fnu4HI GCNGC 4 cut(s) 390, 393, 1097, 1433
FokI GGATG 8 cut(s) 108, 188, 484, 700, 1037, 1055, 1159, 1383
Fsp4HI GCNGC 4 cut(s) 390, 393, 1097, 1433
FspBI CTAG 2 cut(s) 398, 404
GlaI GCGC 1 cut(s) 1431
GluI GCNGC 4 cut(s) 390, 393, 1097, 1433
GsaI CCCAGC 2 cut(s) 261, 806
HaeIII GGCC 3 cut(s) 389, 452, 578
HapII CCGG 1 cut(s) 947
HgaI GACGC 2 cut(s) 821, 1242
HhaI GCGC 1 cut(s) 1432
Hin1II CATG 7 cut(s) 79, 129, 208, 548, 1072, 1180, 1285
Hin6I GCGC 1 cut(s) 1430
HinP1I GCGC 1 cut(s) 1430
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 6 cut(s) 86, 220, 379, 536, 1276, 1397
HpaII CCGG 1 cut(s) 947
HphI GGTGA 7 cut(s) 40, 584, 764, 818, 943, 1207, 1437
Hpy166II GTNNAC 2 cut(s) 241, 599
Hpy188I TCNGA 5 cut(s) 363, 376, 384, 567, 939
Hpy8I GTNNAC 2 cut(s) 241, 599
HpyAV CCTTC 5 cut(s) 75, 347, 379, 680, 965
HpyCH4III ACNGT 2 cut(s) 596, 694
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 8 cut(s) 419, 544, 614, 837, 1127, 1155, 1223, 1285
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 398, 1001, 1010, 1295
HpyF3I CTNAG 6 cut(s) 60, 347, 793, 1014, 1392, 1419
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 7 cut(s) 79, 129, 208, 548, 1072, 1180, 1285
HspAI GCGC 1 cut(s) 1430
Kzo9I GATC 4 cut(s) 562, 745, 1118, 1474
LguI GCTCTTC 1 cut(s) 1305
LmnI GCTCC 1 cut(s) 115
Lsp1109I GCAGC 2 cut(s) 1108, 1444
LweI GCATC 3 cut(s) 30, 166, 1181
MaeI CTAG 2 cut(s) 398, 404
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 6 cut(s) 147, 508, 694, 949, 1254, 1330
MalI GATC 4 cut(s) 564, 747, 1120, 1476
MbiI CCGCTC 1 cut(s) 395
MboI GATC 4 cut(s) 562, 745, 1118, 1474
MfeI CAATTG 2 cut(s) 516, 1128
MflI RGATCY 1 cut(s) 1474
MhlI GDGCHC 2 cut(s) 1006, 1190
MlyI GAGTC 3 cut(s) 229, 545, 1406
MmeI TCCRAC 1 cut(s) 689
Mph1103I ATGCAT 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 5 cut(s) 27, 473, 618, 897, 984
MslI CAYNNNNRTG 3 cut(s) 78, 203, 1193
MspA1I CMGCKG 1 cut(s) 1486
MspI CCGG 1 cut(s) 947
MspR9I CCNGG 2 cut(s) 291, 454
MunI CAATTG 2 cut(s) 516, 1128
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 2 cut(s) 291, 454
MvnI CGCG 1 cut(s) 1253
MwoI GCNNNNNNNGC 5 cut(s) 214, 398, 1001, 1010, 1295
NcoI CCATGG 1 cut(s) 125
NdeII GATC 4 cut(s) 562, 745, 1118, 1474
NlaIII CATG 7 cut(s) 79, 129, 208, 548, 1072, 1180, 1285
NlaIV GGNNCC 3 cut(s) 286, 295, 1476
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 3 cut(s) 694, 949, 1254
NsiI ATGCAT 1 cut(s) 546
NspI RCATGY 3 cut(s) 208, 1072, 1285
OliI CACNNNNGTG 2 cut(s) 78, 1193
PaeI GCATGC 1 cut(s) 1072
PciSI GCTCTTC 1 cut(s) 1305
PctI GAATGC 1 cut(s) 839
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 3 cut(s) 86, 379, 1276
PinAI ACCGGT 1 cut(s) 946
PkrI GCNGC 4 cut(s) 391, 394, 1098, 1434
PleI GAGTC 3 cut(s) 228, 544, 1405
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
PpsI GAGTC 3 cut(s) 228, 544, 1405
Ppu21I YACGTR 1 cut(s) 428
PpuMI RGGWCCY 1 cut(s) 293
PshBI ATTAAT 1 cut(s) 618
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 2 cut(s) 289, 452
PspCI CACGTG 1 cut(s) 428
PspEI GGTNACC 1 cut(s) 949
PspFI CCCAGC 2 cut(s) 257, 802
PspGI CCWGG 2 cut(s) 289, 452
PspN4I GGNNCC 3 cut(s) 286, 295, 1476
PspPI GGNCC 4 cut(s) 293, 450, 456, 577
PspPPI RGGWCCY 1 cut(s) 293
PsrI GAACNNNNNNTAC 4 cut(s) 475, 507, 1230, 1262
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 2 cut(s) 611, 1102
PsuI RGATCY 1 cut(s) 1474
RsaI GTAC 1 cut(s) 1239
RsaNI GTAC 1 cut(s) 1238
RseI CAYNNNNRTG 3 cut(s) 78, 203, 1193
SapI GCTCTTC 1 cut(s) 1305
SaqAI TTAA 5 cut(s) 27, 473, 618, 897, 984
SatI GCNGC 4 cut(s) 390, 393, 1097, 1433
Sau3AI GATC 4 cut(s) 562, 745, 1118, 1474
Sau96I GGNCC 4 cut(s) 293, 450, 456, 577
SbfI CCTGCAGG 1 cut(s) 421
SchI GAGTC 3 cut(s) 229, 545, 1406
ScrFI CCNGG 2 cut(s) 291, 454
SdaI CCTGCAGG 1 cut(s) 421
SduI GDGCHC 2 cut(s) 1006, 1190
SfaNI GCATC 3 cut(s) 30, 166, 1181
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 2 cut(s) 293, 456
SmiMI CAYNNNNRTG 3 cut(s) 78, 203, 1193
SmlI CTYRAG 3 cut(s) 170, 824, 1091
SmoI CTYRAG 3 cut(s) 170, 824, 1091
SphI GCATGC 1 cut(s) 1072
Sse8387I CCTGCAGG 1 cut(s) 421
SsiI CCGC 5 cut(s) 390, 393, 924, 1341, 1486
SspMI CTAG 2 cut(s) 398, 404
StyD4I CCNGG 2 cut(s) 289, 452
StyI CCWWGG 2 cut(s) 125, 446
TaaI ACNGT 2 cut(s) 596, 694
TaiI ACGT 1 cut(s) 430
TaqI TCGA 4 cut(s) 784, 991, 1184, 1500
TauI GCSGC 2 cut(s) 392, 395
TfiI GAWTC 3 cut(s) 86, 379, 1276
Tru1I TTAA 5 cut(s) 27, 473, 618, 897, 984
Tru9I TTAA 5 cut(s) 27, 473, 618, 897, 984
TscAI CASTG 3 cut(s) 286, 652, 736
TseFI GTSAC 3 cut(s) 694, 949, 1254
TseI GCWGC 2 cut(s) 1096, 1432
Tsp45I GTSAC 3 cut(s) 694, 949, 1254
TspDTI ATGAA 2 cut(s) 30, 1260
TspGWI ACGGA 1 cut(s) 1487
TspRI CASTG 3 cut(s) 286, 652, 736
VpaK11BI GGWCC 2 cut(s) 293, 456
VspI ATTAAT 1 cut(s) 618
XapI RAATTY 4 cut(s) 786, 963, 970, 1345
XceI RCATGY 3 cut(s) 208, 1072, 1285
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 2 cut(s) 398, 404
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.