MD12G1119900.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007518
Physical Location & Seq
Forward (+)
19199749 .. 19203748
4000 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1119900.v1.1.491

Sequence Viewer

Length: 1563 bp
ATGGCGACTGAAGATGGAACCTTTGCTAAAAACGGTGGCGGCATTTTCACAAACGAAGCTGAGCCTTCAACCTACATGGATGGAGTTTCCACTGAGATAATTGAAGAAGCACCTCCTGGCCATCTGTGGAGACGACAGAACCTCGTCTTACAGATACCTTCAAGAACTCTTGAGGATGCCAAAGAGAATTTTGTGAGAATAAACATGCCCCCAACGCCGAGCCCCACTCCCAAAAGAGTAAATTTCTCCCCATTACCCAGCCCTAGTTTAGCCAAAATCAATGCGTCCCCAGGTCCCTCGTTGTCAAAAACTAAATCGACCACAAAAAGAAGCCTCCTTCCAAGACTAAGTTTCAAAAATTGGAACACTACTTCGGATATTGAGAAGGCTGCCACTCGAGCACTAGGAGGCTTACCTGCAGGGACACATGAGAAGCCTTCAACTCCAAGGGCCTGGTCCTTTGCAACGCTTTTAACTCCCAGGACGAAAACCGCATCATCCTTGCCTGCAACTCCAATTGCTCACTCAAATCTAGAGTCCATGCATGGCAGGAACACAATTGATCTAAAAGCAGAGGCCCAACGACCTATTTGCCGCTCACGTTCTGTTCCTGCGATTAAGAAAGATGGAAGTATATATGCAGGCAGTGTCATTCGTGTAATTCCAACCACGCCACGAGTGGCTGAGAGGACTGTCACGACTACGTCAAGCACATCCCCAATAAATGTCACTAATGGAAGTGATGATGGTGGTGAAGATATTGCTGAAGAAGAAGCTGTTTGTCGAATTTGCTTAGTTGAGCTAGGTGAAGATGCCGACACCCTCAAGATGGAATGCAGCTGTAAAGGCGACCTTGCTCTTGCCCACCAAAAATGTGCTGTAAAATGGTTCAGCATTAAGGGTAATAAAACATGCGATGTGTGCGGAGAAGATGTTCAGAACCTGCCAGTCACACTTTTACGAATTCAAAATTCTCAGGCTGTTAATTTTCGAGCAAGTAGAGCACAGCAGGCTGAGGTTAATCAATATAGGGTTTGGCAGGATGTTCCGATCCTTGCCATAGTTAGCATGCTTGCTTACTTCTGTTTTCTTGAGCAGCTTCTGGTGGGGAAAATGGGATCGCACGCAATCACTCTCTCTCTTCCTTTTTCCTGCATCTTGGGTCTACTGGCATCCATGACATCGTCCACAATGGTGAGAAGAAGATATGTCTGGAGTTATGCAACTACACAGTTTGCACTGGTCGTCATTGCAGCACATCTACTTTATTCATTGATTCACATGCAGGCAGTTCTGGCTCTTCTCCTCGCCACCTTTTCAGGGTTCGGAATTACAATGTGTGGAAGCTCTATCATTGTTGAGGCTTTGAGATGGAGGGGAAGATGGCGTTCTCGGCAGTCAAATCAACAGCATGGTTCTCAGGAGGTGACACAACCGAATCAATCACCACAGAATACACAACAAACACAAACGAATCCCCGACAGCGGGAAAATGAACCTCAAGGTGCAGAACTTACTCATGGCTCTGCTTCTGTATGCACAAATACCACCGCTGCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

521

Amino Acids

56.66

Weight (kDa)

9.0

Isoelectric Point (pI)

53.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 261 - 308 3.4e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 424, 951
AccBSI CCGCTC 1 cut(s) 597
AccI GTMKAC 1 cut(s) 1165
AciI CCGC 6 cut(s) 39, 492, 595, 924, 1486, 1551
AclWI GGATC 2 cut(s) 1045, 1126
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 5 cut(s) 187, 241, 786, 963, 970
AcuI CTGAAG 2 cut(s) 30, 786
AfiI CCNNNNNNNGG 4 cut(s) 829, 1320, 1485, 1486
AgsI TTSAA 6 cut(s) 69, 104, 162, 355, 441, 968
AjnI CCWGG 4 cut(s) 115, 289, 452, 479
AleI CACNNNNGTG 1 cut(s) 1193
AloI GAACNNNNNNTCC 2 cut(s) 918, 950
AluBI AGCT 6 cut(s) 59, 776, 802, 840, 1099, 1347
AluI AGCT 6 cut(s) 59, 776, 802, 840, 1099, 1347
Alw21I GWGCWC 2 cut(s) 403, 1006
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 2 cut(s) 1045, 1126
AlwNI CAGNNNCTG 2 cut(s) 943, 1102
Ama87I CYCGRG 1 cut(s) 396
AoxI GGCC 3 cut(s) 118, 450, 576
ApeKI GCWGC 5 cut(s) 389, 837, 1096, 1253, 1553
ApoI RAATTY 5 cut(s) 187, 241, 786, 963, 970
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
Asp700I GAANNNNTTC 1 cut(s) 933
AspS9I GGNCC 4 cut(s) 293, 450, 456, 577
AsuHPI GGTGA 5 cut(s) 764, 818, 1207, 1437, 1438
AvaI CYCGRG 1 cut(s) 396
AvaII GGWCC 2 cut(s) 293, 456
BalI TGGCCA 1 cut(s) 120
BanII GRGCYC 1 cut(s) 224
BauI CACGAG 1 cut(s) 675
Bbv12I GWGCWC 2 cut(s) 403, 1006
BbvCI CCTCAGC 1 cut(s) 1014
BbvI GCAGC 5 cut(s) 376, 849, 1108, 1265, 1540
BccI CCATC 8 cut(s) 8, 74, 129, 620, 740, 823, 1365, 1377
BcgI CGANNNNNNTGC 2 cut(s) 573, 607
BciT130I CCWGG 4 cut(s) 117, 291, 454, 481
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 4 cut(s) 264, 404, 533, 803
BfmI CTRYAG 1 cut(s) 417
BfuAI ACCTGC 2 cut(s) 424, 951
BisI GCNGC 7 cut(s) 40, 390, 595, 838, 1097, 1254, 1554
BlpI GCTNAGC 1 cut(s) 60
BlsI GCNGC 7 cut(s) 41, 391, 596, 839, 1098, 1255, 1555
Bme1390I CCNGG 4 cut(s) 117, 291, 454, 481
Bme18I GGWCC 2 cut(s) 293, 456
BmeT110I CYCGRG 1 cut(s) 396
BmgT120I GGNCC 4 cut(s) 293, 450, 456, 577
BmiI GGNNCC 2 cut(s) 19, 295
BmrFI CCNGG 4 cut(s) 117, 291, 454, 481
BmsI GCATC 5 cut(s) 166, 503, 802, 1164, 1181
BplI GAGNNNNNCTC 2 cut(s) 211, 243
BpmI CTGGAG 1 cut(s) 1234
Bpu10I CCTNAGC 1 cut(s) 1014
Bpu1102I GCTNAGC 1 cut(s) 60
BpuEI CTTGAG 4 cut(s) 191, 809, 1112, 1485
BsaJI CCNNGG 3 cut(s) 289, 446, 479
BsaXI ACNNNNNCTCC 2 cut(s) 918, 948
Bsc4I CCNNNNNNNGG 4 cut(s) 829, 1320, 1485, 1486
Bse1I ACTGG 3 cut(s) 947, 1173, 1245
Bse3DI GCAATG 1 cut(s) 1248
BseBI CCWGG 4 cut(s) 117, 291, 454, 481
BseDI CCNNGG 3 cut(s) 289, 446, 479
BseGI GGATG 6 cut(s) 85, 181, 497, 713, 1048, 1172
BseLI CCNNNNNNNGG 4 cut(s) 829, 1320, 1485, 1486
BseMI GCAATG 1 cut(s) 1248
BseMII CTCAG 6 cut(s) 51, 84, 675, 989, 1005, 1433
BseNI ACTGG 3 cut(s) 947, 1173, 1245
BseRI GAGGAG 1 cut(s) 1295
BseXI GCAGC 5 cut(s) 376, 849, 1108, 1265, 1540
BseYI CCCAGC 1 cut(s) 257
BsgI GTGCAG 1 cut(s) 1527
BshFI GGCC 3 cut(s) 120, 452, 578
BsiHKAI GWGCWC 2 cut(s) 403, 1006
BsiHKCI CYCGRG 1 cut(s) 396
BslFI GGGAC 3 cut(s) 271, 279, 436
BslI CCNNNNNNNGG 4 cut(s) 829, 1320, 1485, 1486
BsmAI GTCTC 1 cut(s) 124
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 3 cut(s) 271, 279, 436
BsmI GAATGC 1 cut(s) 839
BsnI GGCC 3 cut(s) 120, 452, 578
BsoBI CYCGRG 1 cut(s) 396
Bsp1286I GDGCHC 3 cut(s) 224, 403, 1006
Bsp143I GATC 3 cut(s) 562, 1050, 1118
Bsp1720I GCTNAGC 1 cut(s) 60
BspACI CCGC 6 cut(s) 39, 492, 595, 924, 1486, 1551
BspANI GGCC 3 cut(s) 120, 452, 578
BspCNI CTCAG 6 cut(s) 52, 85, 676, 988, 1006, 1432
BspLI GGNNCC 2 cut(s) 19, 295
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 2 cut(s) 424, 951
BspPI GGATC 2 cut(s) 1045, 1126
BspQI GCTCTTC 1 cut(s) 1305
BsrBI CCGCTC 1 cut(s) 597
BsrDI GCAATG 1 cut(s) 1248
BsrI ACTGG 3 cut(s) 947, 1173, 1245
BssECI CCNNGG 3 cut(s) 289, 446, 479
BssMI GATC 3 cut(s) 562, 1050, 1118
BssSI CACGAG 1 cut(s) 675
BssT1I CCWWGG 1 cut(s) 446
Bst2BI CACGAG 1 cut(s) 675
Bst2UI CCWGG 4 cut(s) 117, 291, 454, 481
Bst4CI ACNGT 3 cut(s) 35, 694, 1233
Bst6I CTCTTC 2 cut(s) 1146, 1305
BstC8I GCNNGC 8 cut(s) 507, 643, 1011, 1070, 1074, 1125, 1287, 1558
BstDEI CTNAG 8 cut(s) 60, 93, 347, 684, 793, 975, 1014, 1419
BstF5I GGATG 6 cut(s) 85, 181, 497, 713, 1048, 1172
BstKTI GATC 3 cut(s) 565, 1053, 1121
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 3 cut(s) 562, 1050, 1118
BstMWI GCNNNNNNNGC 8 cut(s) 214, 398, 846, 921, 1001, 1010, 1295, 1393
BstNI CCWGG 4 cut(s) 117, 291, 454, 481
BstNSI RCATGY 5 cut(s) 208, 915, 1072, 1285, 1560
BstSCI CCNGG 4 cut(s) 115, 289, 452, 479
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 5 cut(s) 376, 849, 1108, 1265, 1540
BstXI CCANNNNNNTGG 1 cut(s) 453
BsuRI GGCC 3 cut(s) 120, 452, 578
BtgZI GCGATG 1 cut(s) 930
BtsCI GGATG 6 cut(s) 85, 181, 497, 713, 1048, 1172
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 3 cut(s) 90, 652, 1238
BveI ACCTGC 2 cut(s) 424, 951
Cac8I GCNNGC 8 cut(s) 507, 643, 1011, 1070, 1074, 1125, 1287, 1558
CaiI CAGNNNCTG 2 cut(s) 943, 1102
Cfr13I GGNCC 4 cut(s) 293, 450, 456, 577
CseI GACGC 1 cut(s) 273
DdeI CTNAG 8 cut(s) 60, 93, 347, 684, 793, 975, 1014, 1419
DpnI GATC 3 cut(s) 564, 1052, 1120
DpnII GATC 3 cut(s) 562, 1050, 1118
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 2 cut(s) 1146, 1305
EarI CTCTTC 2 cut(s) 1146, 1305
Eco130I CCWWGG 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 293, 456
Eco57I CTGAAG 2 cut(s) 30, 786
Eco88I CYCGRG 1 cut(s) 396
EcoO109I RGGNCCY 2 cut(s) 293, 450
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 4 cut(s) 115, 289, 452, 479
EcoT14I CCWWGG 1 cut(s) 446
EcoT22I ATGCAT 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 446
Esp3I CGTCTC 1 cut(s) 124
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 3 cut(s) 271, 279, 436
FauI CCCGC 1 cut(s) 1479
FblI GTMKAC 1 cut(s) 1165
Fnu4HI GCNGC 7 cut(s) 40, 390, 595, 838, 1097, 1254, 1554
FokI GGATG 6 cut(s) 92, 188, 484, 700, 1055, 1159
FriOI GRGCYC 1 cut(s) 224
Fsp4HI GCNGC 7 cut(s) 40, 390, 595, 838, 1097, 1254, 1554
FspBI CTAG 4 cut(s) 264, 404, 533, 803
GluI GCNGC 7 cut(s) 40, 390, 595, 838, 1097, 1254, 1554
GsaI CCCAGC 1 cut(s) 261
GsuI CTGGAG 1 cut(s) 1234
HaeIII GGCC 3 cut(s) 120, 452, 578
HgaI GACGC 1 cut(s) 273
HinfI GANTC 4 cut(s) 536, 1276, 1438, 1474
HphI GGTGA 5 cut(s) 764, 818, 1207, 1437, 1438
Hpy166II GTNNAC 2 cut(s) 1166, 1188
Hpy188I TCNGA 4 cut(s) 376, 939, 1050, 1328
Hpy188III TCNNGA 8 cut(s) 162, 170, 533, 697, 826, 1091, 1213, 1421
Hpy8I GTNNAC 2 cut(s) 1166, 1188
HpyAV CCTTC 5 cut(s) 75, 168, 347, 379, 447
HpyCH4III ACNGT 3 cut(s) 35, 694, 1233
HpyCH4IV ACGT 2 cut(s) 601, 704
HpyF10VI GCNNNNNNNGC 8 cut(s) 214, 398, 846, 921, 1001, 1010, 1295, 1393
HpyF3I CTNAG 8 cut(s) 60, 93, 347, 684, 793, 975, 1014, 1419
HpySE526I ACGT 2 cut(s) 601, 704
Kzo9I GATC 3 cut(s) 562, 1050, 1118
LguI GCTCTTC 1 cut(s) 1305
Lsp1109I GCAGC 5 cut(s) 376, 849, 1108, 1265, 1540
LweI GCATC 5 cut(s) 166, 503, 802, 1164, 1181
MaeI CTAG 4 cut(s) 264, 404, 533, 803
MaeII ACGT 2 cut(s) 601, 704
MaeIII GTNAC 4 cut(s) 694, 727, 949, 1426
MalI GATC 3 cut(s) 564, 1052, 1120
MbiI CCGCTC 1 cut(s) 597
MboI GATC 3 cut(s) 562, 1050, 1118
MfeI CAATTG 2 cut(s) 516, 558
MhlI GDGCHC 3 cut(s) 224, 403, 1006
MlsI TGGCCA 1 cut(s) 120
MluNI TGGCCA 1 cut(s) 120
MlyI GAGTC 1 cut(s) 545
MmeI TCCRAC 1 cut(s) 689
Mox20I TGGCCA 1 cut(s) 120
Mph1103I ATGCAT 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 933
MscI TGGCCA 1 cut(s) 120
MseI TTAA 5 cut(s) 473, 618, 897, 984, 1020
MslI CAYNNNNRTG 1 cut(s) 1193
Msp20I TGGCCA 1 cut(s) 120
MspA1I CMGCKG 3 cut(s) 840, 1486, 1553
MspR9I CCNGG 4 cut(s) 117, 291, 454, 481
MunI CAATTG 2 cut(s) 516, 558
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 4 cut(s) 117, 291, 454, 481
MwoI GCNNNNNNNGC 8 cut(s) 214, 398, 846, 921, 1001, 1010, 1295, 1393
NdeII GATC 3 cut(s) 562, 1050, 1118
NlaIV GGNNCC 2 cut(s) 19, 295
NmeAIII GCCGAG 2 cut(s) 243, 1372
NmuCI GTSAC 4 cut(s) 694, 727, 949, 1426
NsiI ATGCAT 1 cut(s) 546
NspI RCATGY 5 cut(s) 208, 915, 1072, 1285, 1560
OliI CACNNNNGTG 1 cut(s) 1193
PaeI GCATGC 2 cut(s) 1072, 1560
PaeR7I CTCGAG 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 1305
PctI GAATGC 1 cut(s) 839
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 3 cut(s) 1276, 1438, 1474
PflFI GACNNNGTC 2 cut(s) 703, 1183
PkrI GCNGC 7 cut(s) 41, 391, 596, 839, 1098, 1255, 1555
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
PpuMI RGGWCCY 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 4 cut(s) 115, 289, 452, 479
PspFI CCCAGC 1 cut(s) 257
PspGI CCWGG 4 cut(s) 115, 289, 452, 479
PspN4I GGNNCC 2 cut(s) 19, 295
PspPI GGNCC 4 cut(s) 293, 450, 456, 577
PspPPI RGGWCCY 1 cut(s) 293
PspXI VCTCGAGB 1 cut(s) 396
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 2 cut(s) 943, 1102
PsyI GACNNNGTC 2 cut(s) 703, 1183
PvuII CAGCTG 1 cut(s) 840
RseI CAYNNNNRTG 1 cut(s) 1193
SapI GCTCTTC 1 cut(s) 1305
SaqAI TTAA 5 cut(s) 473, 618, 897, 984, 1020
SatI GCNGC 7 cut(s) 40, 390, 595, 838, 1097, 1254, 1554
Sau3AI GATC 3 cut(s) 562, 1050, 1118
Sau96I GGNCC 4 cut(s) 293, 450, 456, 577
SbfI CCTGCAGG 1 cut(s) 421
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 4 cut(s) 117, 291, 454, 481
SdaI CCTGCAGG 1 cut(s) 421
SduI GDGCHC 3 cut(s) 224, 403, 1006
SfaNI GCATC 5 cut(s) 166, 503, 802, 1164, 1181
SfcI CTRYAG 1 cut(s) 417
Sfr274I CTCGAG 1 cut(s) 396
SinI GGWCC 2 cut(s) 293, 456
SlaI CTCGAG 1 cut(s) 396
SmiMI CAYNNNNRTG 1 cut(s) 1193
SmlI CTYRAG 5 cut(s) 170, 396, 824, 1091, 1500
SmoI CTYRAG 5 cut(s) 170, 396, 824, 1091, 1500
SphI GCATGC 2 cut(s) 1072, 1560
Sse8387I CCTGCAGG 1 cut(s) 421
SsiI CCGC 6 cut(s) 39, 492, 595, 924, 1486, 1551
SspMI CTAG 4 cut(s) 264, 404, 533, 803
StyD4I CCNGG 4 cut(s) 115, 289, 452, 479
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 3 cut(s) 35, 694, 1233
TaiI ACGT 2 cut(s) 604, 707
TaqI TCGA 4 cut(s) 317, 397, 784, 991
TauI GCSGC 2 cut(s) 42, 597
TfiI GAWTC 3 cut(s) 1276, 1438, 1474
Tru1I TTAA 5 cut(s) 473, 618, 897, 984, 1020
Tru9I TTAA 5 cut(s) 473, 618, 897, 984, 1020
TscAI CASTG 3 cut(s) 97, 652, 1245
TseFI GTSAC 4 cut(s) 694, 727, 949, 1426
TseI GCWGC 5 cut(s) 389, 837, 1096, 1253, 1553
Tsp45I GTSAC 4 cut(s) 694, 727, 949, 1426
TspDTI ATGAA 2 cut(s) 1260, 1509
TspRI CASTG 3 cut(s) 97, 652, 1245
Tth111I GACNNNGTC 2 cut(s) 703, 1183
VpaK11BI GGWCC 2 cut(s) 293, 456
XapI RAATTY 5 cut(s) 187, 241, 786, 963, 970
XbaI TCTAGA 1 cut(s) 532
XceI RCATGY 5 cut(s) 208, 915, 1072, 1285, 1560
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XhoI CTCGAG 1 cut(s) 396
XmiI GTMKAC 1 cut(s) 1165
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 4 cut(s) 264, 404, 533, 803
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.