MD04G1099800.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007518
Physical Location & Seq
Forward (+)
18471068 .. 18475179
4112 bp
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UTR
Exon/CDS
Intron
MD04G1099800.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGGCGACTGAAGATGAAAGCATCGTTAAAAATGGTGGCAACATTTTCACCAACGAAGCTGAGCCTTCAGCCCACATGGGTGGAGATTCCACTGAGATAATTGAAGAAGCTCCTCCTGACCATCCATGGAGACGACAGAACCTCGTGTTACAGATACCTCCAAGAACTCTTGAGGATGCCAAAGAAGATTTTGTGAGCATAAACATGCCCCCAACACCGAGTCCCACTCCCAAAAGAGTAAACTTCTCCCCATTACCCAGCCCCATTGTAGCCAAAATCAGTGGTTCCCCAGGTCCCTCATCATCAAAAACTAAATCAACCACAAAAAGAAGCCTCCTTCCAAAACTTAGTTTCAAAAATCGGAACACTACTTCGGAGATTCAGAAGGCTGCTGCTCTAGCACTAGGAGGCTTACCTGCAGGGACACGTGAAAAGCCTACAATTCCAAGGACCTGGTCCTTTACAAAGCTTTTAACTCCCAGGACGAAAAATACATCATCCTTGCCTGTAACTCCAATTGCTCACTCAAATCCAGAGTCTATGCATGGCAGGAACACAACTGATCTGAAAGCAGAGGCCCAACGACCTATTCACCGTTCACATTCAGTCCCTGCGATTAATAAAGATGGAAGCATATATGTAGGCAGTGTCATTCGTGTAATTCCAACCACACCACGAGTGGCTGAAAGGACTGTCACGACAACATCAAGCACATCCCCTGTAAATGACACTGGTGGAAGCGTTGATGGTGGTGAAGATATTGCTGAAGAAGAAGCTGTTTGTCGAATTTGCTTAGTTGAGCTGGGTGAAGACGCTGACACCCTCAAGATGGAATGCAGCTGTAAAGGCGACCTTGCTCTTGCCCACCAAGAATGTGCTGTAAAATGGTTCAGCATTAAAGGTAATAAAAAATGTGATGTGTGTGGAGAAGAGGTTCAGAACCTACCGGTCACCCTTCTACGAATTCAAAATTCTCAGGCCGTTAATTTTCGAGCAAGTAGAGCACAGCAGGCTGAGGCTACTCAATATAGGGTTTGGCAGGATGTTCCCATCCTTGTCATAGTCAGCATGCTTGCTTACTTCTGTTTTCTTGAGCAACTTCTGGTGGGGAAAATGGGATCTGGTGCAATTGCTCTTTCTCTTCCTTTTTCCTGCATTTTGGGTCTTCTGGCATCCATGACATCGACCACGATGGTGAGAAGAAGATATGTCTGGATTTATGCAACTATTCAGTTTGTACTGGTGGTTCTCGCGGCACATCTACTTTATTCATTGATTCACATGCAGGCAGTTCTGGCTCTTCTCCTCGCCACCTTTTCAGGATTTGGAGTTACAATGTGTGGAAGTTCTTTTATTGTCGAGGCTTTGGGATGGAGGGAAAGATGGCGTTCTCAGCAGTCAAATCAACAGCGTGGTTCTCAGGAGGTGGCGCAGCCGAATCAATCACCCCAGAATACACAACATACACAGACGAATCCCCAACAGCGGGAAACTGAACTCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

502

Amino Acids

54.78

Weight (kDa)

8.27

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 261 - 308 3.3e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 424
AccII CGCG 1 cut(s) 1253
AciI CCGC 2 cut(s) 1253, 1486
AclWI GGATC 1 cut(s) 1126
AcsI RAATTY 3 cut(s) 786, 963, 970
AcuI CTGAAG 3 cut(s) 30, 51, 786
AcvI CACGTG 1 cut(s) 428
AfaI GTAC 1 cut(s) 1239
AfiI CCNNNNNNNGG 3 cut(s) 829, 1485, 1486
AflIII ACRYGT 1 cut(s) 425
AgeI ACCGGT 1 cut(s) 946
AgsI TTSAA 3 cut(s) 104, 355, 968
AjnI CCWGG 3 cut(s) 289, 452, 479
AjuI GAANNNNNNNTTGG 2 cut(s) 573, 605
AleI CACNNNNGTG 2 cut(s) 78, 1193
AloI GAACNNNNNNTCC 2 cut(s) 918, 950
AluBI AGCT 6 cut(s) 59, 110, 469, 776, 802, 840
AluI AGCT 6 cut(s) 59, 110, 469, 776, 802, 840
Alw21I GWGCWC 1 cut(s) 1006
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 1 cut(s) 1126
AlwNI CAGNNNCTG 1 cut(s) 611
AoxI GGCC 2 cut(s) 576, 978
ApeKI GCWGC 4 cut(s) 389, 392, 837, 1432
ApoI RAATTY 3 cut(s) 786, 963, 970
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
AseI ATTAAT 1 cut(s) 618
AsiGI ACCGGT 1 cut(s) 946
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 1 cut(s) 1432
AspS9I GGNCC 4 cut(s) 293, 450, 456, 577
AsuHPI GGTGA 7 cut(s) 40, 584, 764, 818, 943, 1207, 1437
AvaII GGWCC 3 cut(s) 293, 450, 456
BauI CACGAG 2 cut(s) 143, 675
BbrPI CACGTG 1 cut(s) 428
BbsI GAAGAC 2 cut(s) 816, 1157
Bbv12I GWGCWC 1 cut(s) 1006
BbvCI CCTCAGC 1 cut(s) 1014
BbvI GCAGC 4 cut(s) 376, 379, 849, 1444
BccI CCATC 8 cut(s) 129, 620, 740, 823, 1058, 1186, 1365, 1377
BceAI ACGGC 1 cut(s) 965
BciT130I CCWGG 3 cut(s) 291, 454, 481
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 2 cut(s) 398, 404
BfmI CTRYAG 1 cut(s) 417
BfuAI ACCTGC 1 cut(s) 424
BisI GCNGC 5 cut(s) 390, 393, 838, 1254, 1433
BlpI GCTNAGC 1 cut(s) 60
BlsI GCNGC 5 cut(s) 391, 394, 839, 1255, 1434
Bme1390I CCNGG 3 cut(s) 291, 454, 481
Bme18I GGWCC 3 cut(s) 293, 450, 456
BmgT120I GGNCC 4 cut(s) 293, 450, 456, 577
BmiI GGNNCC 2 cut(s) 286, 295
BmrFI CCNGG 3 cut(s) 291, 454, 481
BmsI GCATC 3 cut(s) 30, 166, 1181
BpiI GAAGAC 2 cut(s) 816, 1157
BplI GAGNNNNNCTC 2 cut(s) 211, 243
Bpu10I CCTNAGC 1 cut(s) 1014
Bpu1102I GCTNAGC 1 cut(s) 60
BpuEI CTTGAG 3 cut(s) 191, 809, 1112
BsaAI YACGTR 1 cut(s) 428
BsaJI CCNNGG 4 cut(s) 125, 289, 446, 479
BsaWI WCCGGW 1 cut(s) 946
BsaXI ACNNNNNCTCC 2 cut(s) 918, 948
Bsc4I CCNNNNNNNGG 3 cut(s) 829, 1485, 1486
Bse118I RCCGGY 1 cut(s) 946
Bse1I ACTGG 2 cut(s) 736, 1245
BseBI CCWGG 3 cut(s) 291, 454, 481
BseDI CCNNGG 4 cut(s) 125, 289, 446, 479
BseGI GGATG 8 cut(s) 121, 181, 497, 713, 1048, 1050, 1172, 1376
BseLI CCNNNNNNNGG 3 cut(s) 829, 1485, 1486
BseMII CTCAG 6 cut(s) 51, 84, 989, 1005, 1406, 1433
BseNI ACTGG 2 cut(s) 736, 1245
BseRI GAGGAG 2 cut(s) 102, 1295
BseXI GCAGC 4 cut(s) 376, 379, 849, 1444
BseYI CCCAGC 2 cut(s) 257, 802
Bsh1236I CGCG 1 cut(s) 1253
BshFI GGCC 2 cut(s) 578, 980
BshTI ACCGGT 1 cut(s) 946
BsiHKAI GWGCWC 1 cut(s) 1006
BsiSI CCGG 1 cut(s) 947
BslFI GGGAC 4 cut(s) 207, 279, 436, 593
BslI CCNNNNNNNGG 3 cut(s) 829, 1485, 1486
BsmAI GTCTC 1 cut(s) 124
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 4 cut(s) 207, 279, 436, 593
BsmI GAATGC 1 cut(s) 839
BsnI GGCC 2 cut(s) 578, 980
Bsp1286I GDGCHC 1 cut(s) 1006
Bsp143I GATC 2 cut(s) 562, 1118
Bsp1720I GCTNAGC 1 cut(s) 60
Bsp19I CCATGG 1 cut(s) 125
BspACI CCGC 2 cut(s) 1253, 1486
BspANI GGCC 2 cut(s) 578, 980
BspCNI CTCAG 6 cut(s) 52, 85, 988, 1006, 1405, 1432
BspFNI CGCG 1 cut(s) 1253
BspLI GGNNCC 2 cut(s) 286, 295
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 424
BspPI GGATC 1 cut(s) 1126
BspQI GCTCTTC 1 cut(s) 1305
BsrFI RCCGGY 1 cut(s) 946
BsrI ACTGG 2 cut(s) 736, 1245
BssAI RCCGGY 1 cut(s) 946
BssECI CCNNGG 4 cut(s) 125, 289, 446, 479
BssMI GATC 2 cut(s) 562, 1118
BssSI CACGAG 2 cut(s) 143, 675
BssT1I CCWWGG 2 cut(s) 125, 446
Bst2BI CACGAG 2 cut(s) 143, 675
Bst2UI CCWGG 3 cut(s) 291, 454, 481
Bst4CI ACNGT 2 cut(s) 596, 694
Bst6I CTCTTC 3 cut(s) 924, 1146, 1305
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 4 cut(s) 1011, 1070, 1074, 1287
BstDEI CTNAG 8 cut(s) 60, 93, 347, 793, 975, 1014, 1392, 1419
BstDSI CCRYGG 1 cut(s) 125
BstEII GGTNACC 1 cut(s) 949
BstF5I GGATG 8 cut(s) 121, 181, 497, 713, 1048, 1050, 1172, 1376
BstFNI CGCG 1 cut(s) 1253
BstHHI GCGC 1 cut(s) 1432
BstKTI GATC 2 cut(s) 565, 1121
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 2 cut(s) 562, 1118
BstMWI GCNNNNNNNGC 6 cut(s) 398, 846, 1001, 1010, 1295, 1393
BstNI CCWGG 3 cut(s) 291, 454, 481
BstNSI RCATGY 3 cut(s) 208, 1072, 1285
BstPI GGTNACC 1 cut(s) 949
BstSCI CCNGG 3 cut(s) 289, 452, 479
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 1253
BstV1I GCAGC 4 cut(s) 376, 379, 849, 1444
BstV2I GAAGAC 2 cut(s) 816, 1157
BstX2I RGATCY 1 cut(s) 1118
BstXI CCANNNNNNTGG 2 cut(s) 80, 453
BstYI RGATCY 1 cut(s) 1118
BsuRI GGCC 2 cut(s) 578, 980
BtgI CCRYGG 1 cut(s) 125
BtsCI GGATG 8 cut(s) 121, 181, 497, 713, 1048, 1050, 1172, 1376
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 4 cut(s) 90, 286, 652, 729
BveI ACCTGC 1 cut(s) 424
Cac8I GCNNGC 4 cut(s) 1011, 1070, 1074, 1287
CaiI CAGNNNCTG 1 cut(s) 611
CfoI GCGC 1 cut(s) 1432
Cfr10I RCCGGY 1 cut(s) 946
Cfr13I GGNCC 4 cut(s) 293, 450, 456, 577
CseI GACGC 1 cut(s) 821
CsiI ACCWGGT 1 cut(s) 452
Csp6I GTAC 1 cut(s) 1238
CspAI ACCGGT 1 cut(s) 946
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviAII CATG 7 cut(s) 76, 126, 205, 545, 1069, 1177, 1282
CviQI GTAC 1 cut(s) 1238
DdeI CTNAG 8 cut(s) 60, 93, 347, 793, 975, 1014, 1392, 1419
DpnI GATC 2 cut(s) 564, 1120
DpnII GATC 2 cut(s) 562, 1118
Eam1104I CTCTTC 3 cut(s) 924, 1146, 1305
EarI CTCTTC 3 cut(s) 924, 1146, 1305
Eco130I CCWWGG 2 cut(s) 125, 446
Eco47I GGWCC 3 cut(s) 293, 450, 456
Eco57I CTGAAG 3 cut(s) 30, 51, 786
Eco72I CACGTG 1 cut(s) 428
Eco91I GGTNACC 1 cut(s) 949
EcoO109I RGGNCCY 2 cut(s) 293, 450
EcoO65I GGTNACC 1 cut(s) 949
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 3 cut(s) 289, 452, 479
EcoT14I CCWWGG 2 cut(s) 125, 446
EcoT22I ATGCAT 1 cut(s) 546
ErhI CCWWGG 2 cut(s) 125, 446
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 7 cut(s) 79, 129, 208, 548, 1072, 1180, 1285
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 4 cut(s) 207, 279, 436, 593
FatI CATG 7 cut(s) 75, 125, 204, 544, 1068, 1176, 1281
FauI CCCGC 1 cut(s) 1479
Fnu4HI GCNGC 5 cut(s) 390, 393, 838, 1254, 1433
FokI GGATG 8 cut(s) 108, 188, 484, 700, 1037, 1055, 1159, 1383
Fsp4HI GCNGC 5 cut(s) 390, 393, 838, 1254, 1433
FspBI CTAG 2 cut(s) 398, 404
GlaI GCGC 1 cut(s) 1431
GluI GCNGC 5 cut(s) 390, 393, 838, 1254, 1433
GsaI CCCAGC 2 cut(s) 261, 806
HaeIII GGCC 2 cut(s) 578, 980
HapII CCGG 1 cut(s) 947
HgaI GACGC 1 cut(s) 821
HhaI GCGC 1 cut(s) 1432
Hin1II CATG 7 cut(s) 79, 129, 208, 548, 1072, 1180, 1285
Hin6I GCGC 1 cut(s) 1430
HinP1I GCGC 1 cut(s) 1430
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 7 cut(s) 86, 220, 379, 536, 1276, 1438, 1474
HpaII CCGG 1 cut(s) 947
HphI GGTGA 7 cut(s) 40, 584, 764, 818, 943, 1207, 1437
Hpy166II GTNNAC 2 cut(s) 241, 599
Hpy188I TCNGA 5 cut(s) 363, 376, 384, 567, 939
Hpy188III TCNNGA 9 cut(s) 116, 170, 533, 697, 826, 1091, 1213, 1320, 1421
Hpy8I GTNNAC 2 cut(s) 241, 599
HpyAV CCTTC 4 cut(s) 75, 347, 379, 965
HpyCH4III ACNGT 2 cut(s) 596, 694
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 7 cut(s) 419, 544, 837, 1127, 1155, 1223, 1285
HpyF10VI GCNNNNNNNGC 6 cut(s) 398, 846, 1001, 1010, 1295, 1393
HpyF3I CTNAG 8 cut(s) 60, 93, 347, 793, 975, 1014, 1392, 1419
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 7 cut(s) 79, 129, 208, 548, 1072, 1180, 1285
HspAI GCGC 1 cut(s) 1430
Kzo9I GATC 2 cut(s) 562, 1118
LguI GCTCTTC 1 cut(s) 1305
LmnI GCTCC 1 cut(s) 115
Lsp1109I GCAGC 4 cut(s) 376, 379, 849, 1444
LweI GCATC 3 cut(s) 30, 166, 1181
MabI ACCWGGT 1 cut(s) 452
MaeI CTAG 2 cut(s) 398, 404
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 5 cut(s) 147, 508, 694, 949, 1330
MalI GATC 2 cut(s) 564, 1120
MboI GATC 2 cut(s) 562, 1118
MfeI CAATTG 2 cut(s) 516, 1128
MflI RGATCY 1 cut(s) 1118
MhlI GDGCHC 1 cut(s) 1006
MluCI AATT 9 cut(s) 99, 441, 516, 660, 786, 963, 970, 985, 1128
MlyI GAGTC 2 cut(s) 229, 545
MmeI TCCRAC 1 cut(s) 689
Mph1103I ATGCAT 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 5 cut(s) 27, 473, 618, 897, 984
MslI CAYNNNNRTG 3 cut(s) 78, 203, 1193
MspA1I CMGCKG 2 cut(s) 840, 1486
MspI CCGG 1 cut(s) 947
MspR9I CCNGG 3 cut(s) 291, 454, 481
MunI CAATTG 2 cut(s) 516, 1128
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 3 cut(s) 291, 454, 481
MvnI CGCG 1 cut(s) 1253
MwoI GCNNNNNNNGC 6 cut(s) 398, 846, 1001, 1010, 1295, 1393
NcoI CCATGG 1 cut(s) 125
NdeII GATC 2 cut(s) 562, 1118
NlaIII CATG 7 cut(s) 79, 129, 208, 548, 1072, 1180, 1285
NlaIV GGNNCC 2 cut(s) 286, 295
NmuCI GTSAC 2 cut(s) 694, 949
NsiI ATGCAT 1 cut(s) 546
NspI RCATGY 3 cut(s) 208, 1072, 1285
OliI CACNNNNGTG 2 cut(s) 78, 1193
PaeI GCATGC 1 cut(s) 1072
PciSI GCTCTTC 1 cut(s) 1305
PctI GAATGC 1 cut(s) 839
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 5 cut(s) 86, 379, 1276, 1438, 1474
PflFI GACNNNGTC 1 cut(s) 454
PinAI ACCGGT 1 cut(s) 946
PkrI GCNGC 5 cut(s) 391, 394, 839, 1255, 1434
PleI GAGTC 2 cut(s) 228, 544
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
PpsI GAGTC 2 cut(s) 228, 544
Ppu21I YACGTR 1 cut(s) 428
PpuMI RGGWCCY 2 cut(s) 293, 450
PshBI ATTAAT 1 cut(s) 618
Psp5II RGGWCCY 2 cut(s) 293, 450
Psp6I CCWGG 3 cut(s) 289, 452, 479
PspCI CACGTG 1 cut(s) 428
PspEI GGTNACC 1 cut(s) 949
PspFI CCCAGC 2 cut(s) 257, 802
PspGI CCWGG 3 cut(s) 289, 452, 479
PspN4I GGNNCC 2 cut(s) 286, 295
PspPI GGNCC 4 cut(s) 293, 450, 456, 577
PspPPI RGGWCCY 2 cut(s) 293, 450
PsrI GAACNNNNNNTAC 2 cut(s) 1230, 1262
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 611
PsuI RGATCY 1 cut(s) 1118
PsyI GACNNNGTC 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 840
RsaI GTAC 1 cut(s) 1239
RsaNI GTAC 1 cut(s) 1238
RseI CAYNNNNRTG 3 cut(s) 78, 203, 1193
SapI GCTCTTC 1 cut(s) 1305
SaqAI TTAA 5 cut(s) 27, 473, 618, 897, 984
SatI GCNGC 5 cut(s) 390, 393, 838, 1254, 1433
Sau3AI GATC 2 cut(s) 562, 1118
Sau96I GGNCC 4 cut(s) 293, 450, 456, 577
SbfI CCTGCAGG 1 cut(s) 421
SchI GAGTC 2 cut(s) 229, 545
ScrFI CCNGG 3 cut(s) 291, 454, 481
SdaI CCTGCAGG 1 cut(s) 421
SduI GDGCHC 1 cut(s) 1006
SexAI ACCWGGT 1 cut(s) 452
SfaNI GCATC 3 cut(s) 30, 166, 1181
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 3 cut(s) 293, 450, 456
SmiMI CAYNNNNRTG 3 cut(s) 78, 203, 1193
SmlI CTYRAG 3 cut(s) 170, 824, 1091
SmoI CTYRAG 3 cut(s) 170, 824, 1091
SphI GCATGC 1 cut(s) 1072
Sse8387I CCTGCAGG 1 cut(s) 421
Sse9I AATT 9 cut(s) 99, 441, 516, 660, 786, 963, 970, 985, 1128
SsiI CCGC 2 cut(s) 1253, 1486
SspMI CTAG 2 cut(s) 398, 404
StyD4I CCNGG 3 cut(s) 289, 452, 479
StyI CCWWGG 2 cut(s) 125, 446
TaaI ACNGT 2 cut(s) 596, 694
TaiI ACGT 1 cut(s) 430
TaqI TCGA 5 cut(s) 784, 991, 1184, 1359, 1500
TasI AATT 9 cut(s) 99, 441, 516, 660, 786, 963, 970, 985, 1128
TatI WGTACW 1 cut(s) 1237
TauI GCSGC 1 cut(s) 1256
TfiI GAWTC 5 cut(s) 86, 379, 1276, 1438, 1474
Tru1I TTAA 5 cut(s) 27, 473, 618, 897, 984
Tru9I TTAA 5 cut(s) 27, 473, 618, 897, 984
TscAI CASTG 4 cut(s) 97, 286, 652, 736
TseFI GTSAC 2 cut(s) 694, 949
TseI GCWGC 4 cut(s) 389, 392, 837, 1432
Tsp45I GTSAC 2 cut(s) 694, 949
TspDTI ATGAA 2 cut(s) 30, 1260
TspRI CASTG 4 cut(s) 97, 286, 652, 736
Tth111I GACNNNGTC 1 cut(s) 454
VpaK11BI GGWCC 3 cut(s) 293, 450, 456
VspI ATTAAT 1 cut(s) 618
XapI RAATTY 3 cut(s) 786, 963, 970
XceI RCATGY 3 cut(s) 208, 1072, 1285
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 2 cut(s) 398, 404
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.