Rh3DG201000

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0007518
Physical Location & Seq
Forward (+)
18265820 .. 18270290
4471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG201000.1

Sequence Viewer

Length: 1071 bp
ATGGTAATTGAAGAAGAAGCTTCTGCTCAGGAAAATGATGCTGGCATTTCCGCCAGCGAAGTTGACCCTTCACCCCAACAGGATGGAAACTCCACTGAGATAATTGAAGAAACTCCTCCTGGTCACCCATGGAGACGACAAAACCTCGCCTTACAGATACCCACCAGAACTGTTGAAGATCCCGAAGAGGATTTTGTGAGAATAGACATGCCCCCAACGCCAAGTTCCACTCCGAAAAGAGTAAACTTCTCCCCATTACCCAGCCCTAGTTTAAACAAAATCAGTGGATCCCCAGGTCCCTCATCCTCAAAAACTAAGTCAACCATAAAAAGCCTCCTTCCAAAACTAAGTTTCAAGTATCGGAACACTACTTCAGAGATTCAGAAGGCTGCTACTCTTGCACTAGGAGGTACATCTACAGCTACCCGTGAGAAGCCTTCAATTTCTAGGACATTTTCTCTTCCAAAGCTTTTAACACCTAGACTGAAGAATACGTCATCCTTGCCTACAACCCCAATTGCTCACTCAAATCCAGAGTCTATGCATGGAAGAAACACAACTGATCTGAAAGGAGGGCGCCAACTGCATATTCATCGTTCACATTCGGTCCCTGAGCTTAATAAAGATGGGAGTGTAAGTCTAGGTAGTGTCTTTCGAGTAATTCCTACCACACCACGAGCGATGGAGAGAAGTGTATCAATAACTTCATGCACCTCCCCGAAGGATGACAATGATGGAAGTGATGATGATGGTGAAGATATTGCCGAAGAAGAAGCTGTTTGTCGAATTTGCTTAGTTGAATTGGGGGAAGGTGCTGACACCCTCAAGATGGAATGCAGCTGTAAAGGTGACCTCGCTCTAGCCCACCAAGAGTGTGCTGTAAAATGGTTCAGCATTAAAGGTAATAAAACATGTGATGTGTGCAAGCAAGAGGTCCAGAACCTACCTGTCACGCTTTTACGAATTCAAAATGTTCAAGCCCATAATTTACGAGGAACTAGAGCACAGCAGCCTGAGGTTACTCAATATAGGCAAGTGAAATTTTCAACTGCTGTTATGAGCAGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

356

Amino Acids

38.85

Weight (kDa)

6.72

Isoelectric Point (pI)

61.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 261 - 308 4.4e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 576
AciI CCGC 1 cut(s) 51
AclWI GGATC 3 cut(s) 173, 282, 295
AcsI RAATTY 3 cut(s) 786, 963, 1040
AcuI CTGAAG 2 cut(s) 357, 506
AcyI GRCGYC 1 cut(s) 577
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 1 cut(s) 829
AflIII ACRYGT 1 cut(s) 911
AgsI TTSAA 9 cut(s) 11, 107, 176, 355, 441, 800, 968, 977, 1047
AjnI CCWGG 2 cut(s) 118, 292
AjuI GAANNNNNNNTTGG 4 cut(s) 208, 240, 573, 605
AluBI AGCT 6 cut(s) 20, 422, 469, 616, 776, 840
AluI AGCT 6 cut(s) 20, 422, 469, 616, 776, 840
Alw21I GWGCWC 1 cut(s) 1006
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 3 cut(s) 173, 282, 295
ApeKI GCWGC 3 cut(s) 389, 837, 1009
ApoI RAATTY 3 cut(s) 786, 963, 1040
ArsI GACNNNNNNTTYG 4 cut(s) 302, 334, 521, 553
AspLEI GCGC 1 cut(s) 579
AspS9I GGNCC 3 cut(s) 296, 607, 934
AsuHPI GGTGA 4 cut(s) 63, 116, 764, 860
AvaII GGWCC 3 cut(s) 296, 607, 934
AxyI CCTNAGG 1 cut(s) 1014
BamHI GGATCC 1 cut(s) 287
BanI GGYRCC 1 cut(s) 576
BauI CACGAG 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 1006
BbvI GCAGC 3 cut(s) 376, 849, 1021
BccI CCATC 6 cut(s) 77, 620, 676, 728, 743, 823
BcgI CGANNNNNNTGC 2 cut(s) 575, 609
BciT130I CCWGG 2 cut(s) 120, 294
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
BfmI CTRYAG 1 cut(s) 417
BfoI RGCGCY 1 cut(s) 580
BisI GCNGC 3 cut(s) 390, 838, 1010
BlsI GCNGC 3 cut(s) 391, 839, 1011
Bme1390I CCNGG 2 cut(s) 120, 294
Bme18I GGWCC 3 cut(s) 296, 607, 934
BmgT120I GGNCC 3 cut(s) 296, 607, 934
BmiI GGNNCC 4 cut(s) 289, 298, 578, 609
BmrFI CCNGG 2 cut(s) 120, 294
BmsI GCATC 1 cut(s) 28
Bpu10I CCTNAGC 2 cut(s) 27, 612
BpuEI CTTGAG 1 cut(s) 809
BsaHI GRCGYC 1 cut(s) 577
BsaJI CCNNGG 2 cut(s) 128, 292
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 829
Bse21I CCTNAGG 1 cut(s) 1014
BseBI CCWGG 2 cut(s) 120, 294
BseDI CCNNGG 2 cut(s) 128, 292
BseGI GGATG 4 cut(s) 88, 302, 497, 730
BseLI CCNNNNNNNGG 1 cut(s) 829
BseMII CTCAG 4 cut(s) 41, 87, 603, 1005
BseRI GAGGAG 1 cut(s) 105
BseXI GCAGC 3 cut(s) 376, 849, 1021
BseYI CCCAGC 1 cut(s) 260
BshNI GGYRCC 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 1006
BslFI GGGAC 2 cut(s) 282, 593
BslI CCNNNNNNNGG 1 cut(s) 829
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 2 cut(s) 282, 593
BsmI GAATGC 1 cut(s) 839
Bsp1286I GDGCHC 1 cut(s) 1006
Bsp143I GATC 3 cut(s) 178, 287, 562
Bsp19I CCATGG 1 cut(s) 128
BspACI CCGC 1 cut(s) 51
BspCNI CTCAG 4 cut(s) 40, 88, 604, 1006
BspLI GGNNCC 4 cut(s) 289, 298, 578, 609
BspPI GGATC 3 cut(s) 173, 282, 295
BspT107I GGYRCC 1 cut(s) 576
BssECI CCNNGG 2 cut(s) 128, 292
BssMI GATC 3 cut(s) 178, 287, 562
BssNI GRCGYC 1 cut(s) 577
BssSI CACGAG 1 cut(s) 675
BssT1I CCWWGG 1 cut(s) 128
Bst2BI CACGAG 1 cut(s) 675
Bst2UI CCWGG 2 cut(s) 120, 294
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 180, 465
BstACI GRCGYC 1 cut(s) 577
BstC8I GCNNGC 3 cut(s) 43, 55, 926
BstDEI CTNAG 7 cut(s) 27, 96, 315, 347, 612, 793, 1014
BstDSI CCRYGG 1 cut(s) 128
BstEII GGTNACC 2 cut(s) 122, 848
BstF5I GGATG 4 cut(s) 88, 302, 497, 730
BstH2I RGCGCY 1 cut(s) 580
BstHHI GCGC 1 cut(s) 579
BstKTI GATC 3 cut(s) 181, 290, 565
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 3 cut(s) 178, 287, 562
BstMWI GCNNNNNNNGC 3 cut(s) 217, 398, 583
BstNI CCWGG 2 cut(s) 120, 294
BstNSI RCATGY 2 cut(s) 211, 915
BstPI GGTNACC 2 cut(s) 122, 848
BstSCI CCNGG 2 cut(s) 118, 292
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 3 cut(s) 376, 849, 1021
BstX2I RGATCY 2 cut(s) 178, 287
BstXI CCANNNNNNTGG 1 cut(s) 83
BstYI RGATCY 2 cut(s) 178, 287
Bsu36I CCTNAGG 1 cut(s) 1014
BtgI CCRYGG 1 cut(s) 128
BtgZI GCGATG 1 cut(s) 695
BtsCI GGATG 4 cut(s) 88, 302, 497, 730
BtsIMutI CAGTG 2 cut(s) 93, 289
Cac8I GCNNGC 3 cut(s) 43, 55, 926
CfoI GCGC 1 cut(s) 579
Cfr13I GGNCC 3 cut(s) 296, 607, 934
Csp6I GTAC 1 cut(s) 411
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 5 cut(s) 129, 208, 545, 708, 912
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 7 cut(s) 27, 96, 315, 347, 612, 793, 1014
DinI GGCGCC 1 cut(s) 578
DpnI GATC 3 cut(s) 180, 289, 564
DpnII GATC 3 cut(s) 178, 287, 562
DraI TTTAAA 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 180, 465
EarI CTCTTC 2 cut(s) 180, 465
EciI GGCGGA 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 128
Eco47I GGWCC 3 cut(s) 296, 607, 934
Eco57I CTGAAG 2 cut(s) 357, 506
Eco81I CCTNAGG 1 cut(s) 1014
Eco91I GGTNACC 2 cut(s) 122, 848
EcoO109I RGGNCCY 1 cut(s) 296
EcoO65I GGTNACC 2 cut(s) 122, 848
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 2 cut(s) 118, 292
EcoT14I CCWWGG 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 546
EgeI GGCGCC 1 cut(s) 578
EheI GGCGCC 1 cut(s) 578
ErhI CCWWGG 1 cut(s) 128
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 5 cut(s) 132, 211, 548, 711, 915
FaqI GGGAC 2 cut(s) 282, 593
FatI CATG 5 cut(s) 128, 207, 544, 707, 911
Fnu4HI GCNGC 3 cut(s) 390, 838, 1010
FokI GGATG 4 cut(s) 95, 289, 484, 737
Fsp4HI GCNGC 3 cut(s) 390, 838, 1010
FspBI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
GlaI GCGC 1 cut(s) 578
GluI GCNGC 3 cut(s) 390, 838, 1010
GsaI CCCAGC 1 cut(s) 264
HaeII RGCGCY 1 cut(s) 580
HhaI GCGC 1 cut(s) 579
Hin1I GRCGYC 1 cut(s) 577
Hin1II CATG 5 cut(s) 132, 211, 548, 711, 915
Hin6I GCGC 1 cut(s) 577
HinP1I GCGC 1 cut(s) 577
HincII GTYRAC 2 cut(s) 64, 321
HindII GTYRAC 2 cut(s) 64, 321
HindIII AAGCTT 2 cut(s) 18, 467
HinfI GANTC 2 cut(s) 379, 536
HphI GGTGA 4 cut(s) 63, 116, 764, 860
Hpy166II GTNNAC 4 cut(s) 64, 244, 321, 599
Hpy188I TCNGA 5 cut(s) 234, 363, 376, 384, 567
Hpy188III TCNNGA 5 cut(s) 29, 182, 533, 826, 937
Hpy8I GTNNAC 4 cut(s) 64, 244, 321, 599
HpyAV CCTTC 6 cut(s) 78, 347, 379, 447, 715, 803
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 494
HpyCH4V TGCA 6 cut(s) 401, 544, 586, 711, 837, 924
HpyF10VI GCNNNNNNNGC 3 cut(s) 217, 398, 583
HpyF3I CTNAG 7 cut(s) 27, 96, 315, 347, 612, 793, 1014
HpySE526I ACGT 1 cut(s) 494
Hsp92I GRCGYC 1 cut(s) 577
Hsp92II CATG 5 cut(s) 132, 211, 548, 711, 915
HspAI GCGC 1 cut(s) 577
KasI GGCGCC 1 cut(s) 576
Kzo9I GATC 3 cut(s) 178, 287, 562
Lsp1109I GCAGC 3 cut(s) 376, 849, 1021
LweI GCATC 1 cut(s) 28
MaeI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
MaeII ACGT 1 cut(s) 494
MaeIII GTNAC 4 cut(s) 122, 848, 949, 1018
MalI GATC 3 cut(s) 180, 289, 564
MboI GATC 3 cut(s) 178, 287, 562
MfeI CAATTG 1 cut(s) 516
MflI RGATCY 2 cut(s) 178, 287
MhlI GDGCHC 1 cut(s) 1006
Mly113I GGCGCC 1 cut(s) 577
MlyI GAGTC 1 cut(s) 545
Mph1103I ATGCAT 1 cut(s) 546
MseI TTAA 4 cut(s) 272, 473, 618, 897
MspA1I CMGCKG 1 cut(s) 840
MspR9I CCNGG 2 cut(s) 120, 294
MssI GTTTAAAC 1 cut(s) 273
MunI CAATTG 1 cut(s) 516
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 2 cut(s) 120, 294
MwoI GCNNNNNNNGC 3 cut(s) 217, 398, 583
NarI GGCGCC 1 cut(s) 577
NcoI CCATGG 1 cut(s) 128
NdeII GATC 3 cut(s) 178, 287, 562
NlaIII CATG 5 cut(s) 132, 211, 548, 711, 915
NlaIV GGNNCC 4 cut(s) 289, 298, 578, 609
NmuCI GTSAC 3 cut(s) 122, 848, 949
NsiI ATGCAT 1 cut(s) 546
NspI RCATGY 2 cut(s) 211, 915
PciI ACATGT 1 cut(s) 911
PctI GAATGC 1 cut(s) 839
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 3 cut(s) 391, 839, 1011
PleI GAGTC 1 cut(s) 544
PluTI GGCGCC 1 cut(s) 580
PmeI GTTTAAAC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 544
PpuMI RGGWCCY 1 cut(s) 296
PscI ACATGT 1 cut(s) 911
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 118, 292
PspEI GGTNACC 2 cut(s) 122, 848
PspFI CCCAGC 1 cut(s) 260
PspGI CCWGG 2 cut(s) 118, 292
PspN4I GGNNCC 4 cut(s) 289, 298, 578, 609
PspPI GGNCC 3 cut(s) 296, 607, 934
PspPPI RGGWCCY 1 cut(s) 296
PsuI RGATCY 2 cut(s) 178, 287
PvuII CAGCTG 1 cut(s) 840
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
SaqAI TTAA 4 cut(s) 272, 473, 618, 897
SatI GCNGC 3 cut(s) 390, 838, 1010
Sau3AI GATC 3 cut(s) 178, 287, 562
Sau96I GGNCC 3 cut(s) 296, 607, 934
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 2 cut(s) 120, 294
SduI GDGCHC 1 cut(s) 1006
SfaNI GCATC 1 cut(s) 28
SfcI CTRYAG 1 cut(s) 417
SfoI GGCGCC 1 cut(s) 578
SinI GGWCC 3 cut(s) 296, 607, 934
SmlI CTYRAG 1 cut(s) 824
SmoI CTYRAG 1 cut(s) 824
SsiI CCGC 1 cut(s) 51
SspDI GGCGCC 1 cut(s) 576
SspMI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
StyD4I CCNGG 2 cut(s) 118, 292
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 497
TaqI TCGA 2 cut(s) 655, 784
TaqII GACCGA 1 cut(s) 595
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 4 cut(s) 272, 473, 618, 897
Tru9I TTAA 4 cut(s) 272, 473, 618, 897
TscAI CASTG 2 cut(s) 100, 289
TseFI GTSAC 3 cut(s) 122, 848, 949
TseI GCWGC 3 cut(s) 389, 837, 1009
Tsp45I GTSAC 3 cut(s) 122, 848, 949
TspDTI ATGAA 2 cut(s) 581, 696
TspRI CASTG 2 cut(s) 100, 289
VpaK11BI GGWCC 3 cut(s) 296, 607, 934
XapI RAATTY 3 cut(s) 786, 963, 1040
XceI RCATGY 2 cut(s) 211, 915
XspI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.