RchiOBHm_Chr3g0469421

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007518
Physical Location & Seq
Reverse (-)
15167021 .. 15171472
4452 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43526

Sequence Viewer

Length: 1515 bp
ATGGTAATTGAAGAAGAAGCTTCTGCTAAGGAAAATGATGCTGGCATTTCCGCCAGCGAAGTTGACCCTTCACCCCAACAGGATGGAAACTCCAGTGAGATAATTGAAGAAACTCCTCCTGGTCACCCATGGAGACGACAAAACCTCACCTTACAGATACCCACCAGAACTGTTGAGGATCCCGAAGAGGATTTTGTGAGAATAGACATGCCCCCAACGCCAAGTTCCACTCCGAAAAGAGTAAACTTCTCCCCATTACCCAGCCCTAGTTTAAACAAAATCAGTGGATCCCCAGGTCCCTCATCCTCAAAAACTAAGTCAACCATAAAAAACCTCCTTCCAAAACTAAGTTTCAAGTATCGGAACACTACTTCAGAGATTCGGAAGGCTGCTACTCTTGCACTAGGAGGTACATCTACAGCTACCCGTGAGAAGCCTTCAATTTCTAGGACACTTTCTCTTCCAAAGCTTTTAGCACCTAGACTGAAGAATACGTCATCCTTGCCTACAACCCCAATTGCTCACTCAAATCCAGAGTCTATGCATGGAAGAAACACAACTGATCTGAAAGGAGGGCGCCAACTGCCTATTCATCGTTCACATTCGGTCCCTGAGCTTAACAAAGATGGGAGTGTAAGTCTAGGTAGTGTCTTTCGAGTAATTCCTACCACACCACGAGCAATGGAGAGAAGTGTATCAATAACTTCATGCACCTCCCCGAAGGATGAAAATGATGGAAGTGATGATGATGGTGAAGATATTGCCGAAGAAGAAGCTGTTTGTCGAATTTGCTTAGTTGAATTGGGGGAAGGTGCTGACACCCTCAAGATGGAATGCAGCTGTAAAGGTGACCTCGCTCTAGCCCACCAAGAGTGTGCTGTAAAATGGTTCAGCATTAAAGGTAATAAAACATGTGATGTGTGCAAGCAAGAGGTCCAGAACCTACCTGTCACGCTTTTACGAATTCAAAATGTTCAAGCCCATAATTTACGAGGAACTAGAGCACAGCAGCCTGAGGTTACTCAATATAGGGTTTGGCAGGATGTTCCAATTCTCGTCATAGTTAGCATGCTCGCGTACTTCTGTTTTCTTGAGCAGCTTCTGGTAGGAAAAATGGGATCAGGTGCAATTGCGCTCTCTCTTCCTTTTTCCTGTATATTGGGTCTTTTGGCATCCATGACAGCAACCACAATGGTGAAAAGAAAATACGTCTGGGTTTATGCAACCATTCAGTTTGCCTTGGTGGTTCTCTCAGCTCATTTATTTTATTCATTGATTCACATGCAGGCAGTTCTGTCAGTTCTTCTTGCCACGTTTACCGGATTTGGAGTCACAATGTGTGGGAATTCTATTATTATTGAGGCTCTGGAATGGAGGGAAAGATGGCTTTCTCGGTCAAATCAACAGCATGGTACTCCGGCGGTGACCCAGCCAAATCAACCACAACAAACACAGACAAATCCTCGGCAGCTTGAAAATGAAATTCAAGGTGCTGAAGCTATTCATGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

504

Amino Acids

55.16

Weight (kDa)

6.62

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 261 - 308 7.2e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 576
AccII CGCG 1 cut(s) 1076
AciI CCGC 2 cut(s) 51, 1421
AclWI GGATC 5 cut(s) 173, 186, 282, 295, 1126
AcsI RAATTY 4 cut(s) 786, 963, 1345, 1482
AcuI CTGAAG 3 cut(s) 357, 506, 1515
AcyI GRCGYC 1 cut(s) 577
AdeI CACNNNGTG 1 cut(s) 1338
AfaI GTAC 3 cut(s) 412, 1079, 1414
AfiI CCNNNNNNNGG 1 cut(s) 829
AflIII ACRYGT 1 cut(s) 911
AgsI TTSAA 9 cut(s) 11, 107, 355, 441, 800, 968, 977, 1475, 1487
AjnI CCWGG 2 cut(s) 118, 292
AjuI GAANNNNNNNTTGG 4 cut(s) 208, 240, 573, 605
AleI CACNNNNGTG 1 cut(s) 1193
Alw21I GWGCWC 1 cut(s) 1006
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 5 cut(s) 173, 186, 282, 295, 1126
AlwNI CAGNNNCTG 1 cut(s) 1102
ApeKI GCWGC 6 cut(s) 389, 837, 1009, 1096, 1468, 1509
ApoI RAATTY 4 cut(s) 786, 963, 1345, 1482
ArsI GACNNNNNNTTYG 4 cut(s) 302, 334, 521, 553
Asp700I GAANNNNTTC 1 cut(s) 1500
AspLEI GCGC 2 cut(s) 579, 1135
AspS9I GGNCC 3 cut(s) 296, 607, 934
AsuHPI GGTGA 7 cut(s) 63, 116, 139, 764, 860, 1207, 1435
AvaII GGWCC 3 cut(s) 296, 607, 934
AxyI CCTNAGG 1 cut(s) 1014
BamHI GGATCC 2 cut(s) 178, 287
BanI GGYRCC 1 cut(s) 576
BauI CACGAG 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 1006
BbvI GCAGC 5 cut(s) 376, 849, 1021, 1108, 1480
BccI CCATC 6 cut(s) 77, 620, 728, 743, 823, 1377
BciT130I CCWGG 2 cut(s) 120, 294
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
BfmI CTRYAG 1 cut(s) 417
BfoI RGCGCY 1 cut(s) 580
BisI GCNGC 6 cut(s) 390, 838, 1010, 1097, 1469, 1510
BlsI GCNGC 6 cut(s) 391, 839, 1011, 1098, 1470, 1511
Bme1390I CCNGG 2 cut(s) 120, 294
Bme18I GGWCC 3 cut(s) 296, 607, 934
BmgT120I GGNCC 3 cut(s) 296, 607, 934
BmiI GGNNCC 5 cut(s) 180, 289, 298, 578, 609
BmrFI CCNGG 2 cut(s) 120, 294
BmsI GCATC 2 cut(s) 28, 1181
BpmI CTGGAG 1 cut(s) 76
Bpu10I CCTNAGC 2 cut(s) 27, 612
BpuEI CTTGAG 2 cut(s) 809, 1112
BsaHI GRCGYC 1 cut(s) 577
BsaJI CCNNGG 4 cut(s) 128, 292, 1239, 1463
BsaWI WCCGGW 1 cut(s) 1319
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 829
Bse1I ACTGG 1 cut(s) 93
Bse21I CCTNAGG 1 cut(s) 1014
Bse3DI GCAATG 1 cut(s) 687
BseBI CCWGG 2 cut(s) 120, 294
BseDI CCNNGG 4 cut(s) 128, 292, 1239, 1463
BseGI GGATG 6 cut(s) 88, 302, 497, 730, 1048, 1172
BseLI CCNNNNNNNGG 1 cut(s) 829
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 3 cut(s) 603, 1005, 1266
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 105
BseXI GCAGC 5 cut(s) 376, 849, 1021, 1108, 1480
BseYI CCCAGC 2 cut(s) 260, 1428
Bsh1236I CGCG 1 cut(s) 1076
BshNI GGYRCC 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 1006
BsiSI CCGG 2 cut(s) 1320, 1418
BslFI GGGAC 2 cut(s) 282, 593
BslI CCNNNNNNNGG 1 cut(s) 829
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 2 cut(s) 282, 593
BsmI GAATGC 1 cut(s) 839
Bsp1286I GDGCHC 1 cut(s) 1006
Bsp143I GATC 4 cut(s) 178, 287, 562, 1118
Bsp19I CCATGG 1 cut(s) 128
BspACI CCGC 2 cut(s) 51, 1421
BspCNI CTCAG 3 cut(s) 604, 1006, 1265
BspFNI CGCG 1 cut(s) 1076
BspLI GGNNCC 5 cut(s) 180, 289, 298, 578, 609
BspPI GGATC 5 cut(s) 173, 186, 282, 295, 1126
BspT107I GGYRCC 1 cut(s) 576
BsrDI GCAATG 1 cut(s) 687
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 4 cut(s) 128, 292, 1239, 1463
BssMI GATC 4 cut(s) 178, 287, 562, 1118
BssNI GRCGYC 1 cut(s) 577
BssSI CACGAG 1 cut(s) 675
BssT1I CCWWGG 2 cut(s) 128, 1239
Bst2BI CACGAG 1 cut(s) 675
Bst2UI CCWGG 2 cut(s) 120, 294
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 3 cut(s) 180, 465, 1146
BstACI GRCGYC 1 cut(s) 577
BstC8I GCNNGC 6 cut(s) 43, 55, 926, 1070, 1074, 1287
BstDEI CTNAG 7 cut(s) 27, 315, 347, 612, 793, 1014, 1252
BstDSI CCRYGG 1 cut(s) 128
BstEII GGTNACC 3 cut(s) 122, 848, 1423
BstF5I GGATG 6 cut(s) 88, 302, 497, 730, 1048, 1172
BstFNI CGCG 1 cut(s) 1076
BstH2I RGCGCY 1 cut(s) 580
BstHHI GCGC 2 cut(s) 579, 1135
BstKTI GATC 4 cut(s) 181, 290, 565, 1121
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 4 cut(s) 178, 287, 562, 1118
BstMWI GCNNNNNNNGC 3 cut(s) 217, 398, 583
BstNI CCWGG 2 cut(s) 120, 294
BstNSI RCATGY 4 cut(s) 211, 915, 1072, 1285
BstPI GGTNACC 3 cut(s) 122, 848, 1423
BstSCI CCNGG 2 cut(s) 118, 292
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 1076
BstV1I GCAGC 5 cut(s) 376, 849, 1021, 1108, 1480
BstX2I RGATCY 2 cut(s) 178, 287
BstXI CCANNNNNNTGG 1 cut(s) 83
BstYI RGATCY 2 cut(s) 178, 287
Bsu36I CCTNAGG 1 cut(s) 1014
BtgI CCRYGG 1 cut(s) 128
BtsCI GGATG 6 cut(s) 88, 302, 497, 730, 1048, 1172
BtsIMutI CAGTG 2 cut(s) 100, 289
Cac8I GCNNGC 6 cut(s) 43, 55, 926, 1070, 1074, 1287
CaiI CAGNNNCTG 1 cut(s) 1102
CfoI GCGC 2 cut(s) 579, 1135
Cfr13I GGNCC 3 cut(s) 296, 607, 934
Csp6I GTAC 3 cut(s) 411, 1078, 1413
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviQI GTAC 3 cut(s) 411, 1078, 1413
DdeI CTNAG 7 cut(s) 27, 315, 347, 612, 793, 1014, 1252
DinI GGCGCC 1 cut(s) 578
DpnI GATC 4 cut(s) 180, 289, 564, 1120
DpnII GATC 4 cut(s) 178, 287, 562, 1118
DraI TTTAAA 1 cut(s) 273
DraIII CACNNNGTG 1 cut(s) 1338
Eam1104I CTCTTC 3 cut(s) 180, 465, 1146
EarI CTCTTC 3 cut(s) 180, 465, 1146
EciI GGCGGA 1 cut(s) 40
Eco130I CCWWGG 2 cut(s) 128, 1239
Eco47I GGWCC 3 cut(s) 296, 607, 934
Eco57I CTGAAG 3 cut(s) 357, 506, 1515
Eco81I CCTNAGG 1 cut(s) 1014
Eco91I GGTNACC 3 cut(s) 122, 848, 1423
EcoO109I RGGNCCY 1 cut(s) 296
EcoO65I GGTNACC 3 cut(s) 122, 848, 1423
EcoRI GAATTC 2 cut(s) 963, 1345
EcoRII CCWGG 2 cut(s) 118, 292
EcoT14I CCWWGG 2 cut(s) 128, 1239
EcoT22I ATGCAT 1 cut(s) 546
EgeI GGCGCC 1 cut(s) 578
EheI GGCGCC 1 cut(s) 578
ErhI CCWWGG 2 cut(s) 128, 1239
Esp3I CGTCTC 1 cut(s) 127
FaqI GGGAC 2 cut(s) 282, 593
Fnu4HI GCNGC 6 cut(s) 390, 838, 1010, 1097, 1469, 1510
FokI GGATG 6 cut(s) 95, 289, 484, 737, 1055, 1159
Fsp4HI GCNGC 6 cut(s) 390, 838, 1010, 1097, 1469, 1510
FspBI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
GlaI GCGC 2 cut(s) 578, 1134
GluI GCNGC 6 cut(s) 390, 838, 1010, 1097, 1469, 1510
GsaI CCCAGC 2 cut(s) 264, 1432
GsuI CTGGAG 1 cut(s) 76
HaeII RGCGCY 1 cut(s) 580
HapII CCGG 2 cut(s) 1320, 1418
HhaI GCGC 2 cut(s) 579, 1135
Hin1I GRCGYC 1 cut(s) 577
Hin6I GCGC 2 cut(s) 577, 1133
HinP1I GCGC 2 cut(s) 577, 1133
HincII GTYRAC 2 cut(s) 64, 321
HindII GTYRAC 2 cut(s) 64, 321
HindIII AAGCTT 2 cut(s) 18, 467
HinfI GANTC 4 cut(s) 379, 536, 1276, 1329
HpaII CCGG 2 cut(s) 1320, 1418
HphI GGTGA 7 cut(s) 63, 116, 139, 764, 860, 1207, 1435
Hpy166II GTNNAC 5 cut(s) 64, 244, 321, 599, 1317
Hpy188I TCNGA 5 cut(s) 234, 363, 376, 384, 567
Hpy188III TCNNGA 6 cut(s) 182, 533, 826, 937, 1091, 1367
Hpy8I GTNNAC 5 cut(s) 64, 244, 321, 599, 1317
HpyAV CCTTC 6 cut(s) 78, 347, 379, 447, 715, 803
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 3 cut(s) 494, 1209, 1313
HpyCH4V TGCA 8 cut(s) 401, 544, 711, 837, 924, 1127, 1223, 1285
HpyF10VI GCNNNNNNNGC 3 cut(s) 217, 398, 583
HpyF3I CTNAG 7 cut(s) 27, 315, 347, 612, 793, 1014, 1252
HpySE526I ACGT 3 cut(s) 494, 1209, 1313
Hsp92I GRCGYC 1 cut(s) 577
HspAI GCGC 2 cut(s) 577, 1133
KasI GGCGCC 1 cut(s) 576
Kzo9I GATC 4 cut(s) 178, 287, 562, 1118
Lsp1109I GCAGC 5 cut(s) 376, 849, 1021, 1108, 1480
LweI GCATC 2 cut(s) 28, 1181
MaeI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
MaeII ACGT 3 cut(s) 494, 1209, 1313
MaeIII GTNAC 6 cut(s) 122, 848, 949, 1018, 1330, 1423
MalI GATC 4 cut(s) 180, 289, 564, 1120
MboI GATC 4 cut(s) 178, 287, 562, 1118
MfeI CAATTG 2 cut(s) 516, 1128
MflI RGATCY 2 cut(s) 178, 287
MhlI GDGCHC 1 cut(s) 1006
Mly113I GGCGCC 1 cut(s) 577
MlyI GAGTC 2 cut(s) 545, 1338
Mph1103I ATGCAT 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 1500
MseI TTAA 3 cut(s) 272, 618, 897
MslI CAYNNNNRTG 1 cut(s) 1193
MspA1I CMGCKG 2 cut(s) 840, 1512
MspI CCGG 2 cut(s) 1320, 1418
MspR9I CCNGG 2 cut(s) 120, 294
MssI GTTTAAAC 1 cut(s) 273
MunI CAATTG 2 cut(s) 516, 1128
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 2 cut(s) 120, 294
MvnI CGCG 1 cut(s) 1076
MwoI GCNNNNNNNGC 3 cut(s) 217, 398, 583
NarI GGCGCC 1 cut(s) 577
NcoI CCATGG 1 cut(s) 128
NdeII GATC 4 cut(s) 178, 287, 562, 1118
NlaIV GGNNCC 5 cut(s) 180, 289, 298, 578, 609
NmeAIII GCCGAG 1 cut(s) 1444
NmuCI GTSAC 5 cut(s) 122, 848, 949, 1330, 1423
NsiI ATGCAT 1 cut(s) 546
NspI RCATGY 4 cut(s) 211, 915, 1072, 1285
OliI CACNNNNGTG 1 cut(s) 1193
PaeI GCATGC 1 cut(s) 1072
PciI ACATGT 1 cut(s) 911
PctI GAATGC 1 cut(s) 839
PdmI GAANNNNTTC 1 cut(s) 1500
PfeI GAWTC 2 cut(s) 379, 1276
PkrI GCNGC 6 cut(s) 391, 839, 1011, 1098, 1470, 1511
PleI GAGTC 2 cut(s) 544, 1337
PluTI GGCGCC 1 cut(s) 580
PmeI GTTTAAAC 1 cut(s) 273
PpsI GAGTC 2 cut(s) 544, 1337
PpuMI RGGWCCY 1 cut(s) 296
PscI ACATGT 1 cut(s) 911
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 118, 292
PspEI GGTNACC 3 cut(s) 122, 848, 1423
PspFI CCCAGC 2 cut(s) 260, 1428
PspGI CCWGG 2 cut(s) 118, 292
PspN4I GGNNCC 5 cut(s) 180, 289, 298, 578, 609
PspPI GGNCC 3 cut(s) 296, 607, 934
PspPPI RGGWCCY 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 1102
PsuI RGATCY 2 cut(s) 178, 287
PvuII CAGCTG 2 cut(s) 840, 1512
RsaI GTAC 3 cut(s) 412, 1079, 1414
RsaNI GTAC 3 cut(s) 411, 1078, 1413
RseI CAYNNNNRTG 1 cut(s) 1193
SaqAI TTAA 3 cut(s) 272, 618, 897
SatI GCNGC 6 cut(s) 390, 838, 1010, 1097, 1469, 1510
Sau3AI GATC 4 cut(s) 178, 287, 562, 1118
Sau96I GGNCC 3 cut(s) 296, 607, 934
SchI GAGTC 2 cut(s) 545, 1338
ScrFI CCNGG 2 cut(s) 120, 294
SduI GDGCHC 1 cut(s) 1006
SfaNI GCATC 2 cut(s) 28, 1181
SfcI CTRYAG 1 cut(s) 417
SfoI GGCGCC 1 cut(s) 578
SinI GGWCC 3 cut(s) 296, 607, 934
SmiMI CAYNNNNRTG 1 cut(s) 1193
SmlI CTYRAG 2 cut(s) 824, 1091
SmoI CTYRAG 2 cut(s) 824, 1091
SphI GCATGC 1 cut(s) 1072
SsiI CCGC 2 cut(s) 51, 1421
SspDI GGCGCC 1 cut(s) 576
SspMI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
StyD4I CCNGG 2 cut(s) 118, 292
StyI CCWWGG 2 cut(s) 128, 1239
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 3 cut(s) 497, 1212, 1316
TaqI TCGA 2 cut(s) 655, 784
TaqII GACCGA 2 cut(s) 595, 1383
TfiI GAWTC 2 cut(s) 379, 1276
Tru1I TTAA 3 cut(s) 272, 618, 897
Tru9I TTAA 3 cut(s) 272, 618, 897
TscAI CASTG 2 cut(s) 100, 289
TseFI GTSAC 5 cut(s) 122, 848, 949, 1330, 1423
TseI GCWGC 6 cut(s) 389, 837, 1009, 1096, 1468, 1509
Tsp45I GTSAC 5 cut(s) 122, 848, 949, 1330, 1423
TspDTI ATGAA 6 cut(s) 581, 696, 741, 1260, 1493, 1494
TspRI CASTG 2 cut(s) 100, 289
VpaK11BI GGWCC 3 cut(s) 296, 607, 934
XapI RAATTY 4 cut(s) 786, 963, 1345, 1482
XceI RCATGY 4 cut(s) 211, 915, 1072, 1285
XmnI GAANNNNTTC 1 cut(s) 1500
XspI CTAG 7 cut(s) 267, 404, 447, 480, 641, 860, 999
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.