FvH4_6g17171

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016881
Physical Location & Seq
Forward (+)
11157181 .. 11158568
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17171.t1

Sequence Viewer

Length: 975 bp
ATGGAGGACGTTGCATCCAACTTTCGTCCTTCTTCATCGTCCTACTTCACACCCCTTTTGATTTCCATGGCCGGAATAGTCTCCACTACTCTAGCAGTAATAGCATACCATTTCATAGTGGTGAAGTTTTGCATGAGGCATAGGTATAGGCAGAGACAGAGGATACTCGAAGCATTCTCTAATCTCTCACCGCAACAAAATCCCAACATCAACCAAAGTGGGGTGGATGAGAAAGTTTTGAGCTCTATCCCAATTCTCTCTTACTCCACAAAAGACGGACTAACCGGTCTAGATCAGAGTGAGTGTGTGATCTGCTTGGGTGAGCTGGAAGATGGTGACATGGTGAGGCTTCTCCCCAATTGCAAACATGTGTTTCACAATCCATGCATAGACATATGGTTCCTGGCACATACAAACTGTCCAATTTGTAGGGCACCAATAGCTGCGCCCATTAAAACTGGGGAACATTTGACTGCAGATCAAGAGATCCCAGATCAAGAAGTTCGAGATCAATCCAGCAGCGTCGAGTGTTCTAATTCGAATTCGACCTCGAACTTGTCTACCCAGAGTCATGAGGAATTAAGGGGTCTACTTAGCGTCCAATTGCCAAAACCAAATGGTTTGCTAAGGAATTGTGTCTCACTTGTGTTATTACCTATTGATGGGAGTAATAAGCAGCAGAGGTTTGTGAATGGCTTGCAGAGGTCTTTGTCTTTGGATCCGTCACATATCCTCATAAACATACAGAGAGAATGTGATGATGATCACAAACCCTCTTCATCATCTTCATCATCATCGTCTTCTTCTTCTCCTCCTCCTCACACTAAAGCTGTTCTCGTAGAGTGTGGATCATTTAAGGAACGATCTTTCAGACAGTTTGATCGGATGTCGACAGTGTTAATACAATCTCTTTCGCAATTGCGGGTGGGGCAAAGTAGCAGCATGGCAAATGGTCATGGAATTCTTCCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

325

Amino Acids

35.68

Weight (kDa)

6.41

Isoelectric Point (pI)

62.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 101 - 144 2.8e-13 Ring finger domain
zf-RING_11 PF17123 101 - 130 1.7e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 101 - 143 2.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 101 - 144 1.8e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016881)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17171
malus_domestica MD12G1103100.v1.1 MD14G1097100.v1.1
prunus_persica Prupe.7G123200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471271
rosa_laevigata RLG00000024192
rosa_multiflora Rmu_ssc0000091.1_g000001
rosa_roxburghii Rroxscaffold_6G00410150
rosa_rugosa Rorug03G0116700
rosa_samantha Rh3AG166000 Rh3BG191400 Rh3CG181700 Rh3DG184300
rosa_wichuraiana Rw3G015620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 433
AccI GTMKAC 3 cut(s) 560, 589, 890
AciI CCGC 2 cut(s) 191, 922
AclWI GGATC 4 cut(s) 481, 713, 726, 856
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 541, 960
AfiI CCNNNNNNNGG 2 cut(s) 220, 662
AflIII ACRYGT 1 cut(s) 367
AgeI ACCGGT 1 cut(s) 284
AjnI CCWGG 1 cut(s) 402
AluBI AGCT 4 cut(s) 243, 325, 443, 830
AluI AGCT 4 cut(s) 243, 325, 443, 830
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 3 cut(s) 85, 148, 643
AlwI GGATC 4 cut(s) 481, 713, 726, 856
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 4 cut(s) 443, 519, 676, 939
ApoI RAATTY 2 cut(s) 541, 960
AsiGI ACCGGT 1 cut(s) 284
AspLEI GCGC 1 cut(s) 448
AsuHPI GGTGA 5 cut(s) 133, 180, 332, 347, 355
AsuII TTCGAA 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 436
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 245
BbsI GAAGAC 1 cut(s) 792
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 4 cut(s) 430, 531, 688, 951
BccI CCATC 2 cut(s) 326, 656
BciT130I CCWGG 1 cut(s) 404
BciVI GTATCC 1 cut(s) 156
BclI TGATCA 1 cut(s) 763
BcoDI GTCTC 3 cut(s) 85, 148, 643
BfaI CTAG 2 cut(s) 92, 290
BfmI CTRYAG 1 cut(s) 474
BfuI GTATCC 1 cut(s) 156
BisI GCNGC 4 cut(s) 444, 520, 677, 940
BlsI GCNGC 4 cut(s) 445, 521, 678, 941
Bme1390I CCNGG 1 cut(s) 404
BmiI GGNNCC 3 cut(s) 401, 435, 720
BmrFI CCNGG 1 cut(s) 404
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 468
BpiI GAAGAC 1 cut(s) 792
Bpu10I CCTNAGC 1 cut(s) 626
Bpu14I TTCGAA 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 762
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 662
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 463
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 1 cut(s) 404
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 3 cut(s) 14, 232, 891
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 2 cut(s) 220, 662
BseNI ACTGG 1 cut(s) 463
BseRI GAGGAG 3 cut(s) 801, 804, 807
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 4 cut(s) 430, 531, 688, 951
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 1 cut(s) 433
BshTI ACCGGT 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 2 cut(s) 72, 285
BslI CCNNNNNNNGG 2 cut(s) 220, 662
BsmAI GTCTC 3 cut(s) 85, 148, 643
BsmI GAATGC 1 cut(s) 173
BsnI GGCC 1 cut(s) 71
Bsp119I TTCGAA 1 cut(s) 539
Bsp1286I GDGCHC 2 cut(s) 245, 436
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 2 cut(s) 191, 922
BspANI GGCC 1 cut(s) 71
BspHI TCATGA 1 cut(s) 571
BspLI GGNNCC 3 cut(s) 401, 435, 720
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 4 cut(s) 481, 713, 726, 856
BspT104I TTCGAA 1 cut(s) 539
BspT107I GGYRCC 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 463
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 3 cut(s) 419, 876, 895
Bst6I CTCTTC 1 cut(s) 781
BstBI TTCGAA 1 cut(s) 539
BstC8I GCNNGC 1 cut(s) 698
BstDEI CTNAG 2 cut(s) 593, 626
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 3 cut(s) 14, 232, 891
BstHHI GCGC 1 cut(s) 448
BstMAI GTCTC 3 cut(s) 85, 148, 643
BstMWI GCNNNNNNNGC 3 cut(s) 101, 440, 928
BstNI CCWGG 1 cut(s) 404
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 402
BstSFI CTRYAG 1 cut(s) 474
BstSLI GKGCMC 1 cut(s) 436
BstV1I GCAGC 4 cut(s) 430, 531, 688, 951
BstV2I GAAGAC 1 cut(s) 792
BstX2I RGATCY 2 cut(s) 486, 718
BstYI RGATCY 2 cut(s) 486, 718
BsuI GTATCC 1 cut(s) 156
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 3 cut(s) 14, 232, 891
BtsIMutI CAGTG 1 cut(s) 900
Cac8I GCNNGC 1 cut(s) 698
CciI TCATGA 1 cut(s) 571
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 1 cut(s) 284
CseI GACGC 2 cut(s) 511, 586
CspAI ACCGGT 1 cut(s) 284
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 8 cut(s) 67, 133, 340, 368, 384, 572, 943, 956
CviJI RGCY 7 cut(s) 71, 243, 325, 349, 443, 696, 830
CviKI_1 RGCY 7 cut(s) 71, 243, 325, 349, 443, 696, 830
DdeI CTNAG 2 cut(s) 593, 626
DrdI GACNNNNNNGTC 1 cut(s) 285
DseDI GACNNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 781
EarI CTCTTC 1 cut(s) 781
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 243
EcoICRI GAGCTC 1 cut(s) 243
EcoRI GAATTC 2 cut(s) 541, 960
EcoRII CCWGG 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 389
EcoT38I GRGCYC 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 8 cut(s) 70, 136, 343, 371, 387, 575, 946, 959
FatI CATG 8 cut(s) 66, 132, 339, 367, 383, 571, 942, 955
FauI CCCGC 1 cut(s) 915
FauNDI CATATG 1 cut(s) 395
FbaI TGATCA 1 cut(s) 763
FblI GTMKAC 3 cut(s) 560, 589, 890
Fnu4HI GCNGC 4 cut(s) 444, 520, 677, 940
FokI GGATG 2 cut(s) 239, 898
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 4 cut(s) 444, 520, 677, 940
FspBI CTAG 2 cut(s) 92, 290
GlaI GCGC 1 cut(s) 447
GluI GCNGC 4 cut(s) 444, 520, 677, 940
HaeIII GGCC 1 cut(s) 71
HapII CCGG 2 cut(s) 72, 285
HgaI GACGC 2 cut(s) 511, 586
HhaI GCGC 1 cut(s) 448
Hin1II CATG 8 cut(s) 70, 136, 343, 371, 387, 575, 946, 959
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HincII GTYRAC 1 cut(s) 891
HindII GTYRAC 1 cut(s) 891
HinfI GANTC 1 cut(s) 568
HpaII CCGG 2 cut(s) 72, 285
HphI GGTGA 5 cut(s) 133, 180, 332, 347, 355
Hpy166II GTNNAC 3 cut(s) 561, 590, 891
Hpy188I TCNGA 3 cut(s) 297, 872, 885
Hpy188III TCNNGA 5 cut(s) 290, 482, 497, 506, 572
Hpy8I GTNNAC 3 cut(s) 561, 590, 891
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 3 cut(s) 419, 876, 895
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 6 cut(s) 14, 132, 363, 387, 476, 700
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 440, 928
HpyF3I CTNAG 2 cut(s) 593, 626
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 8 cut(s) 70, 136, 343, 371, 387, 575, 946, 959
HspAI GCGC 1 cut(s) 446
Ksp22I TGATCA 1 cut(s) 763
LpnPI CCDG 9 cut(s) 85, 298, 311, 389, 416, 444, 504, 529, 578
Lsp1109I GCAGC 4 cut(s) 430, 531, 688, 951
LweI GCATC 1 cut(s) 23
MaeI CTAG 2 cut(s) 92, 290
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 2 cut(s) 335, 723
MboII GAAGA 8 cut(s) 24, 341, 768, 777, 792, 795, 798, 956
MfeI CAATTG 3 cut(s) 358, 602, 917
MflI RGATCY 2 cut(s) 486, 718
MhlI GDGCHC 2 cut(s) 245, 436
MlyI GAGTC 1 cut(s) 577
MmeI TCCRAC 1 cut(s) 42
Mph1103I ATGCAT 1 cut(s) 389
MseI TTAA 4 cut(s) 453, 581, 855, 899
MslI CAYNNNNRTG 1 cut(s) 119
MspI CCGG 2 cut(s) 72, 285
MspR9I CCNGG 1 cut(s) 404
MunI CAATTG 3 cut(s) 358, 602, 917
Mva1269I GAATGC 1 cut(s) 173
MvaI CCWGG 1 cut(s) 404
MwoI GCNNNNNNNGC 3 cut(s) 101, 440, 928
NcoI CCATGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 395
NlaIII CATG 8 cut(s) 70, 136, 343, 371, 387, 575, 946, 959
NlaIV GGNNCC 3 cut(s) 401, 435, 720
NmuCI GTSAC 2 cut(s) 335, 723
NsiI ATGCAT 1 cut(s) 389
NspI RCATGY 1 cut(s) 371
NspV TTCGAA 1 cut(s) 539
PagI TCATGA 1 cut(s) 571
PciI ACATGT 1 cut(s) 367
PctI GAATGC 1 cut(s) 173
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 4 cut(s) 445, 521, 678, 941
PleI GAGTC 1 cut(s) 576
PpsI GAGTC 1 cut(s) 576
PscI ACATGT 1 cut(s) 367
Psp124BI GAGCTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 402
PspGI CCWGG 1 cut(s) 402
PspN4I GGNNCC 3 cut(s) 401, 435, 720
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 2 cut(s) 486, 718
RseI CAYNNNNRTG 1 cut(s) 119
SacI GAGCTC 1 cut(s) 245
SalI GTCGAC 1 cut(s) 889
SaqAI TTAA 4 cut(s) 453, 581, 855, 899
SatI GCNGC 4 cut(s) 444, 520, 677, 940
SchI GAGTC 1 cut(s) 577
ScrFI CCNGG 1 cut(s) 404
SduI GDGCHC 2 cut(s) 245, 436
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 1 cut(s) 474
SfuI TTCGAA 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 119
SsiI CCGC 2 cut(s) 191, 922
SspMI CTAG 2 cut(s) 92, 290
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 402
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 3 cut(s) 419, 876, 895
TaiI ACGT 1 cut(s) 12
TaqI TCGA 7 cut(s) 168, 505, 525, 539, 545, 551, 890
Tru1I TTAA 4 cut(s) 453, 581, 855, 899
Tru9I TTAA 4 cut(s) 453, 581, 855, 899
TscAI CASTG 1 cut(s) 900
TseFI GTSAC 2 cut(s) 335, 723
TseI GCWGC 4 cut(s) 443, 519, 676, 939
Tsp45I GTSAC 2 cut(s) 335, 723
TspDTI ATGAA 4 cut(s) 24, 103, 768, 777
TspGWI ACGGA 2 cut(s) 291, 711
TspRI CASTG 1 cut(s) 900
XapI RAATTY 2 cut(s) 541, 960
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 1 cut(s) 371
XmiI GTMKAC 3 cut(s) 560, 589, 890
XspI CTAG 2 cut(s) 92, 290
Zsp2I ATGCAT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.