MD12G1103100.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016881
Physical Location & Seq
Reverse (-)
16154741 .. 16155718
978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1103100.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGAGAATGTTGGGATAAGAGTGGAAGGAGCTTCAGGTTCTGGTTCTTTCTTCACACCCCTTTTGATTTCCATGGCAGGCATTGTCTTCACAACCCTAGCAATAGTTGCGTACCATTTCCTAGTTAAGTATTGCCTACGGAGGAGGCAACAGCAGATGCAACTGCAAATCCAAGCCAGCCTCGCGCAACAATCAGGCACCAATCAATCCAATGGGGTTGATGAGAAAGTCCTGAATTCCATCCCAATTCTCTCGTACTCGACGAACGATGGTGAACTTTTTCGCGTGGATCAAAGCGAGTGCGTGATTTGCTTGGGAGACTTAGAAGACGGGGATTTGGTGCGTTTGTTGCCGAGTTGCAAGCACGTGTTTCATAATCCATGCATAGAAAGGTGGTTTTGGGGGCATACAAACTGTCCGGTTTGTAGGTCAGCTATAGATGTGCCTATTGCTTCTGTGGCATCAACGTTGCCTGTGGAATATAGTCATGATCAAGTTGGTCAAGTTAGAGACAGAGAACTCGAAGAAGCCGGCCCAGTATATCAACCCAATTGCTCATCGCACCCTCATGATCCAGAGGAGGGTTTGGCAATTACAGCTGCTAGTACTACTATATCAACCCAATTGTCAAAACCAAATGGTTTGCTTAGGCATTGTGTGTCACTGGTGGTATTGCCTATGGAGGATAGTAGTAAGCAGCAGATTTTGTTGAAGAGGTTGGAGAGGTCTTTGTCTTTGGATCATTCCCACGTTCTTATCAATATATACACAGAGAGTGATGACAAGCCCTCTTCATCATCTTCTACTTCTTCTGATTGTTCCTCTTCGAAGCCGGTTCTTAAGCAGTGTAGATCGTTGAAGGCGCGGTCGATGAGGCAGCTTGATCGAATGTCTTCAGTTTTAGTAAAATCTATTTCTCAACTGCGGATGGGCCAGAGTTTCAGCACGGCCAGTGGCCATGGAAATCTTCCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

326

Amino Acids

35.68

Weight (kDa)

6.41

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 100 - 143 4.8e-13 Ring finger domain
zf-RING_11 PF17123 100 - 129 9.9e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 100 - 142 3.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 100 - 143 6.5e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 101 - 142 1.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016881)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17171
malus_domestica MD12G1103100.v1.1 MD14G1097100.v1.1
prunus_persica Prupe.7G123200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471271
rosa_laevigata RLG00000024192
rosa_multiflora Rmu_ssc0000091.1_g000001
rosa_roxburghii Rroxscaffold_6G00410150
rosa_rugosa Rorug03G0116700
rosa_samantha Rh3AG166000 Rh3BG191400 Rh3CG181700 Rh3DG184300
rosa_wichuraiana Rw3G015620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 197
AccII CGCG 3 cut(s) 185, 285, 865
AciI CCGC 2 cut(s) 865, 925
AclI AACGTT 1 cut(s) 467
AclWI GGATC 3 cut(s) 297, 566, 747
AcoI YGGCCR 2 cut(s) 948, 955
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 2 cut(s) 18, 879
AcvI CACGTG 1 cut(s) 367
AfaI GTAC 3 cut(s) 113, 257, 607
AfiI CCNNNNNNNGG 1 cut(s) 581
AflII CTTAAG 1 cut(s) 839
AflIII ACRYGT 1 cut(s) 366
AgsI TTSAA 2 cut(s) 712, 859
AluBI AGCT 4 cut(s) 32, 434, 599, 880
AluI AGCT 4 cut(s) 32, 434, 599, 880
Alw26I GTCTC 2 cut(s) 312, 504
AlwI GGATC 3 cut(s) 297, 566, 747
AlwNI CAGNNNCTG 1 cut(s) 41
AoxI GGCC 4 cut(s) 532, 931, 948, 955
ApeKI GCWGC 3 cut(s) 599, 697, 877
ApoI RAATTY 1 cut(s) 235
Asp700I GAANNNNTTC 2 cut(s) 279, 892
AspLEI GCGC 2 cut(s) 187, 865
AspS9I GGNCC 2 cut(s) 533, 931
AsuHPI GGTGA 1 cut(s) 284
AsuII TTCGAA 1 cut(s) 827
BalI TGGCCA 1 cut(s) 957
BanI GGYRCC 1 cut(s) 197
BbrPI CACGTG 1 cut(s) 367
BbsI GAAGAC 3 cut(s) 79, 333, 885
BbvI GCAGC 3 cut(s) 586, 709, 889
BccI CCATC 3 cut(s) 248, 263, 922
BceAI ACGGC 1 cut(s) 963
BcgI CGANNNNNNTGC 2 cut(s) 866, 900
BclI TGATCA 1 cut(s) 490
BcoDI GTCTC 2 cut(s) 312, 504
BfaI CTAG 3 cut(s) 98, 122, 603
BfmI CTRYAG 1 cut(s) 435
BfrI CTTAAG 1 cut(s) 839
BisI GCNGC 3 cut(s) 600, 698, 878
BlsI GCNGC 3 cut(s) 601, 699, 879
BmcAI AGTACT 1 cut(s) 607
BmgT120I GGNCC 2 cut(s) 533, 931
BmiI GGNNCC 1 cut(s) 199
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 2 cut(s) 147, 470
BmuI ACTGGG 1 cut(s) 530
BpiI GAAGAC 3 cut(s) 79, 333, 885
Bpu10I CCTNAGC 1 cut(s) 647
Bpu14I TTCGAA 1 cut(s) 827
BsaAI YACGTR 1 cut(s) 367
BsaJI CCNNGG 2 cut(s) 72, 958
BsaWI WCCGGW 1 cut(s) 418
Bsc4I CCNNNNNNNGG 1 cut(s) 581
Bse118I RCCGGY 2 cut(s) 530, 832
Bse1I ACTGG 3 cut(s) 536, 669, 951
BseDI CCNNGG 2 cut(s) 72, 958
BseGI GGATG 2 cut(s) 240, 933
BseLI CCNNNNNNNGG 1 cut(s) 581
BseNI ACTGG 3 cut(s) 536, 669, 951
BseRI GAGGAG 2 cut(s) 157, 593
BseXI GCAGC 3 cut(s) 586, 709, 889
Bsh1236I CGCG 3 cut(s) 185, 285, 865
Bsh1285I CGRYCG 1 cut(s) 869
BshFI GGCC 4 cut(s) 534, 933, 950, 957
BshNI GGYRCC 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 869
BsiSI CCGG 3 cut(s) 419, 531, 833
BslI CCNNNNNNNGG 1 cut(s) 581
BsmAI GTCTC 2 cut(s) 312, 504
BsnI GGCC 4 cut(s) 534, 933, 950, 957
Bsp119I TTCGAA 1 cut(s) 827
Bsp143I GATC 6 cut(s) 289, 490, 571, 739, 851, 883
Bsp19I CCATGG 2 cut(s) 72, 958
BspACI CCGC 2 cut(s) 865, 925
BspANI GGCC 4 cut(s) 534, 933, 950, 957
BspFNI CGCG 3 cut(s) 185, 285, 865
BspHI TCATGA 2 cut(s) 487, 568
BspLI GGNNCC 1 cut(s) 199
BspPI GGATC 3 cut(s) 297, 566, 747
BspT104I TTCGAA 1 cut(s) 827
BspT107I GGYRCC 1 cut(s) 197
BspTI CTTAAG 1 cut(s) 839
BsrFI RCCGGY 2 cut(s) 530, 832
BsrI ACTGG 3 cut(s) 536, 669, 951
BssAI RCCGGY 2 cut(s) 530, 832
BssECI CCNNGG 2 cut(s) 72, 958
BssMI GATC 6 cut(s) 289, 490, 571, 739, 851, 883
BssT1I CCWWGG 2 cut(s) 72, 958
Bst4CI ACNGT 1 cut(s) 416
Bst6I CTCTTC 3 cut(s) 707, 796, 829
BstAFI CTTAAG 1 cut(s) 839
BstAPI GCANNNNNTGC 1 cut(s) 107
BstBAI YACGTR 1 cut(s) 367
BstBI TTCGAA 1 cut(s) 827
BstC8I GCNNGC 4 cut(s) 79, 178, 362, 532
BstDEI CTNAG 2 cut(s) 322, 647
BstDSI CCRYGG 2 cut(s) 72, 958
BstF5I GGATG 2 cut(s) 240, 933
BstFNI CGCG 3 cut(s) 185, 285, 865
BstHHI GCGC 2 cut(s) 187, 865
BstKTI GATC 6 cut(s) 292, 493, 574, 742, 854, 886
BstMAI GTCTC 2 cut(s) 312, 504
BstMBI GATC 6 cut(s) 289, 490, 571, 739, 851, 883
BstMCI CGRYCG 1 cut(s) 869
BstMWI GCNNNNNNNGC 6 cut(s) 107, 182, 309, 349, 458, 596
BstSFI CTRYAG 1 cut(s) 435
BstUI CGCG 3 cut(s) 185, 285, 865
BstV1I GCAGC 3 cut(s) 586, 709, 889
BstV2I GAAGAC 3 cut(s) 79, 333, 885
BsuRI GGCC 4 cut(s) 534, 933, 950, 957
BtgI CCRYGG 2 cut(s) 72, 958
BtgZI GCGATG 1 cut(s) 543
BtsCI GGATG 2 cut(s) 240, 933
BtsI GCAGTG 1 cut(s) 851
BtsIMutI CAGTG 3 cut(s) 662, 851, 958
Cac8I GCNNGC 4 cut(s) 79, 178, 362, 532
CaiI CAGNNNCTG 1 cut(s) 41
CciI TCATGA 2 cut(s) 487, 568
CfoI GCGC 2 cut(s) 187, 865
Cfr10I RCCGGY 2 cut(s) 530, 832
Cfr13I GGNCC 2 cut(s) 533, 931
Csp6I GTAC 3 cut(s) 112, 256, 606
CviAII CATG 5 cut(s) 73, 381, 488, 569, 959
CviQI GTAC 3 cut(s) 112, 256, 606
DdeI CTNAG 2 cut(s) 322, 647
DpnI GATC 6 cut(s) 291, 492, 573, 741, 853, 885
DpnII GATC 6 cut(s) 289, 490, 571, 739, 851, 883
EaeI YGGCCR 2 cut(s) 948, 955
Eam1104I CTCTTC 3 cut(s) 707, 796, 829
EarI CTCTTC 3 cut(s) 707, 796, 829
Eco130I CCWWGG 2 cut(s) 72, 958
Eco57I CTGAAG 2 cut(s) 18, 879
Eco72I CACGTG 1 cut(s) 367
EcoRI GAATTC 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 72, 958
EcoT22I ATGCAT 1 cut(s) 386
ErhI CCWWGG 2 cut(s) 72, 958
FaeI CATG 5 cut(s) 76, 384, 491, 572, 962
FatI CATG 5 cut(s) 72, 380, 487, 568, 958
FbaI TGATCA 1 cut(s) 490
Fnu4HI GCNGC 3 cut(s) 600, 698, 878
FokI GGATG 2 cut(s) 227, 940
Fsp4HI GCNGC 3 cut(s) 600, 698, 878
FspBI CTAG 3 cut(s) 98, 122, 603
GlaI GCGC 2 cut(s) 186, 864
GluI GCNGC 3 cut(s) 600, 698, 878
HaeIII GGCC 4 cut(s) 534, 933, 950, 957
HapII CCGG 3 cut(s) 419, 531, 833
HhaI GCGC 2 cut(s) 187, 865
Hin1II CATG 5 cut(s) 76, 384, 491, 572, 962
Hin6I GCGC 2 cut(s) 185, 863
HinP1I GCGC 2 cut(s) 185, 863
HpaII CCGG 3 cut(s) 419, 531, 833
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 275
Hpy188I TCNGA 1 cut(s) 814
Hpy188III TCNNGA 4 cut(s) 232, 488, 569, 575
Hpy8I GTNNAC 1 cut(s) 275
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 2 cut(s) 20, 853
HpyCH4III ACNGT 1 cut(s) 416
HpyCH4IV ACGT 3 cut(s) 366, 467, 750
HpyCH4V TGCA 4 cut(s) 160, 166, 360, 384
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 182, 309, 349, 458, 596
HpyF3I CTNAG 2 cut(s) 322, 647
HpySE526I ACGT 3 cut(s) 366, 467, 750
Hsp92II CATG 5 cut(s) 76, 384, 491, 572, 962
HspAI GCGC 2 cut(s) 185, 863
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 6 cut(s) 289, 490, 571, 739, 851, 883
LmnI GCTCC 1 cut(s) 29
Lsp1109I GCAGC 3 cut(s) 586, 709, 889
LweI GCATC 2 cut(s) 147, 470
MaeI CTAG 3 cut(s) 98, 122, 603
MaeII ACGT 3 cut(s) 366, 467, 750
MaeIII GTNAC 1 cut(s) 660
MalI GATC 6 cut(s) 291, 492, 573, 741, 853, 885
MboI GATC 6 cut(s) 289, 490, 571, 739, 851, 883
MfeI CAATTG 2 cut(s) 550, 623
MlsI TGGCCA 1 cut(s) 957
MluCI AATT 5 cut(s) 235, 246, 550, 591, 623
MluNI TGGCCA 1 cut(s) 957
MmeI TCCRAC 1 cut(s) 699
Mox20I TGGCCA 1 cut(s) 957
Mph1103I ATGCAT 1 cut(s) 386
MroNI GCCGGC 1 cut(s) 530
MroXI GAANNNNTTC 2 cut(s) 279, 892
MscI TGGCCA 1 cut(s) 957
MseI TTAA 2 cut(s) 126, 840
MslI CAYNNNNRTG 1 cut(s) 567
Msp20I TGGCCA 1 cut(s) 957
MspA1I CMGCKG 1 cut(s) 599
MspCI CTTAAG 1 cut(s) 839
MspI CCGG 3 cut(s) 419, 531, 833
MunI CAATTG 2 cut(s) 550, 623
MvnI CGCG 3 cut(s) 185, 285, 865
MwoI GCNNNNNNNGC 6 cut(s) 107, 182, 309, 349, 458, 596
NaeI GCCGGC 1 cut(s) 532
NcoI CCATGG 2 cut(s) 72, 958
NdeII GATC 6 cut(s) 289, 490, 571, 739, 851, 883
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 5 cut(s) 76, 384, 491, 572, 962
NlaIV GGNNCC 1 cut(s) 199
NmeAIII GCCGAG 1 cut(s) 378
NmuCI GTSAC 1 cut(s) 660
NsiI ATGCAT 1 cut(s) 386
NspV TTCGAA 1 cut(s) 827
PagI TCATGA 2 cut(s) 487, 568
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PdiI GCCGGC 1 cut(s) 532
PdmI GAANNNNTTC 2 cut(s) 279, 892
PkrI GCNGC 3 cut(s) 601, 699, 879
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
Ppu21I YACGTR 1 cut(s) 367
Psp1406I AACGTT 1 cut(s) 467
PspCI CACGTG 1 cut(s) 367
PspN4I GGNNCC 1 cut(s) 199
PspPI GGNCC 2 cut(s) 533, 931
PstNI CAGNNNCTG 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 3 cut(s) 113, 257, 607
RsaNI GTAC 3 cut(s) 112, 256, 606
RseI CAYNNNNRTG 1 cut(s) 567
SaqAI TTAA 2 cut(s) 126, 840
SatI GCNGC 3 cut(s) 600, 698, 878
Sau3AI GATC 6 cut(s) 289, 490, 571, 739, 851, 883
Sau96I GGNCC 2 cut(s) 533, 931
ScaI AGTACT 1 cut(s) 607
SfaNI GCATC 2 cut(s) 147, 470
SfcI CTRYAG 1 cut(s) 435
SfuI TTCGAA 1 cut(s) 827
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 5 cut(s) 235, 246, 550, 591, 623
SsiI CCGC 2 cut(s) 865, 925
SspMI CTAG 3 cut(s) 98, 122, 603
StyI CCWWGG 2 cut(s) 72, 958
TaaI ACNGT 1 cut(s) 416
TaiI ACGT 3 cut(s) 369, 470, 753
TaqI TCGA 5 cut(s) 260, 522, 827, 869, 886
TasI AATT 5 cut(s) 235, 246, 550, 591, 623
TatI WGTACW 1 cut(s) 605
Tru1I TTAA 2 cut(s) 126, 840
Tru9I TTAA 2 cut(s) 126, 840
TscAI CASTG 3 cut(s) 669, 851, 958
TseFI GTSAC 1 cut(s) 660
TseI GCWGC 3 cut(s) 599, 697, 877
Tsp45I GTSAC 1 cut(s) 660
TspDTI ATGAA 2 cut(s) 362, 783
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 3 cut(s) 669, 851, 958
Vha464I CTTAAG 1 cut(s) 839
XapI RAATTY 1 cut(s) 235
XmnI GAANNNNTTC 2 cut(s) 279, 892
XspI CTAG 3 cut(s) 98, 122, 603
ZrmI AGTACT 1 cut(s) 607
Zsp2I ATGCAT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.