MD14G1097100.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016881
Physical Location & Seq
Forward (+)
14744355 .. 14745365
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1097100.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGGAGAATGTTGGGACTCAAGTGGTAGGAGCTTCAGGTTCGGGTTCTTTCTTCACACCCCTTTTGATTTCCATGGCCGGCATTGTCTTCACAACCCTAGCAATAGTTGCGTACCATTTCCTAGTCGTCAAGTATTTCCTGCGGAGGAGGCAACGGCAGATGCAACTGCAATTTCAAGCCAGCCTCGCGCAACAACCAGCCACCAGCCAATCCATTGGGGTTGATGAGAAAGTCTTGAATACGATTCCGATTCTCACGTATTCAATGGAAGATGGTGAAATTTTTCGCATGGATCAGAGCGAGTGTGTGATTTGCTTGGGAGACCTAGAAGACGGGGATTTGGTGCGTTTGATGCCGAGTTGCAAGCATGTGTTTCACAATCCATGCATAGAGACGTGGTTTATGGGGCATTCTAATTGTCCGGTTTGTAGGTCAGCTATAGATGTGCCTATTGTGCCTGTTTCATCAACGTTGCCTGTGGAATATGATCATGATCATGATGATCAAGTTAGAGACCAAGAACTCGAAGAAGCAGGCCCGGTTAGTTGCTCATCGCACCCTCACGATCCAGAGGAGGGTTTACCAATTAGCACTGCTACCACTACTACAACTCAATTATCAAAACCAAATGGTTTGCTTAGGCATTGCGTGTCACTGGTGGTATTGCCTATGAAGGATAGTAGTAAGCAACAAATTTTGTTAAAGGGGTTGGAGAGGTCTTTGTCTTTGGATCATTCCCACGTTCTTATCAATATATACACAGATAGTGATGACAAGCCATCTTCATCATCATCATCATCATCATCATCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGATTGTGCTTCTTCGAAGCCAGTTCTTACTAATTGTGGATCGTTGAAGGTGCGGTCGATGAGGCAGCTTGATCGAATGTCTTCAGTTTTCGTAAACTCTATTTCTCAACTGCGGAAGAGCCAGAGTTTCAGCACGGACAATGGCCATGGAAATCTTCCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

337

Amino Acids

36.56

Weight (kDa)

5.59

Isoelectric Point (pI)

61.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 101 - 144 2.1e-13 Ring finger domain
zf-RING_11 PF17123 101 - 130 1.8e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 101 - 143 3.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 101 - 144 2.2e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 102 - 143 2.6e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016881)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17171
malus_domestica MD12G1103100.v1.1 MD14G1097100.v1.1
prunus_persica Prupe.7G123200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471271
rosa_laevigata RLG00000024192
rosa_multiflora Rmu_ssc0000091.1_g000001
rosa_roxburghii Rroxscaffold_6G00410150
rosa_rugosa Rorug03G0116700
rosa_samantha Rh3AG166000 Rh3BG191400 Rh3CG181700 Rh3DG184300
rosa_wichuraiana Rw3G015620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 188
AciI CCGC 3 cut(s) 142, 898, 958
AclI AACGTT 1 cut(s) 470
AclWI GGATC 4 cut(s) 300, 560, 738, 892
AcoI YGGCCR 2 cut(s) 75, 988
AcsI RAATTY 2 cut(s) 279, 693
AcuI CTGAAG 2 cut(s) 18, 912
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 1 cut(s) 575
AgsI TTSAA 4 cut(s) 176, 238, 264, 892
AjiI CACGTC 1 cut(s) 396
AluBI AGCT 3 cut(s) 32, 437, 913
AluI AGCT 3 cut(s) 32, 437, 913
Alw26I GTCTC 3 cut(s) 315, 386, 507
AlwI GGATC 4 cut(s) 300, 560, 738, 892
AoxI GGCC 3 cut(s) 75, 535, 988
ApeKI GCWGC 1 cut(s) 910
ApoI RAATTY 2 cut(s) 279, 693
Asp700I GAANNNNTTC 2 cut(s) 282, 925
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 536
AsuC2I CCSGG 1 cut(s) 539
AsuHPI GGTGA 1 cut(s) 287
AsuII TTCGAA 1 cut(s) 860
BalI TGGCCA 1 cut(s) 990
BbsI GAAGAC 3 cut(s) 79, 336, 918
BbvI GCAGC 1 cut(s) 922
BccI CCATC 2 cut(s) 266, 787
BceAI ACGGC 1 cut(s) 170
BcgI CGANNNNNNTGC 2 cut(s) 899, 933
BclI TGATCA 3 cut(s) 487, 493, 502
BcnI CCSGG 1 cut(s) 539
BcoDI GTCTC 3 cut(s) 315, 386, 507
BfaI CTAG 3 cut(s) 98, 122, 326
BfmI CTRYAG 1 cut(s) 438
BisI GCNGC 1 cut(s) 911
BlsI GCNGC 1 cut(s) 912
Bme1390I CCNGG 1 cut(s) 539
BmgBI CACGTC 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 536
BmrFI CCNGG 1 cut(s) 539
BmsI GCATC 2 cut(s) 150, 342
BpiI GAAGAC 3 cut(s) 79, 336, 918
Bpu10I CCTNAGC 1 cut(s) 638
Bpu14I TTCGAA 1 cut(s) 860
BpuMI CCSGG 1 cut(s) 539
BsaAI YACGTR 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 492
BsaI GGTCTC 2 cut(s) 315, 507
BsaJI CCNNGG 2 cut(s) 72, 991
BsaWI WCCGGW 1 cut(s) 421
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 660, 866
Bse3DI GCAATG 1 cut(s) 643
Bse8I GATNNNNATC 1 cut(s) 492
BseDI CCNNGG 2 cut(s) 72, 991
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMI GCAATG 1 cut(s) 643
BseNI ACTGG 2 cut(s) 660, 866
BseRI GAGGAG 2 cut(s) 160, 587
BseXI GCAGC 1 cut(s) 922
Bsh1236I CGCG 1 cut(s) 188
Bsh1285I CGRYCG 1 cut(s) 902
BshFI GGCC 3 cut(s) 77, 537, 990
BsiEI CGRYCG 1 cut(s) 902
BsiSI CCGG 3 cut(s) 78, 422, 539
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 1 cut(s) 575
BsmAI GTCTC 3 cut(s) 315, 386, 507
BsmBI CGTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 28
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 3 cut(s) 77, 537, 990
Bso31I GGTCTC 2 cut(s) 315, 507
Bsp119I TTCGAA 1 cut(s) 860
Bsp143I GATC 8 cut(s) 292, 487, 493, 502, 565, 730, 884, 916
Bsp19I CCATGG 2 cut(s) 72, 991
BspACI CCGC 3 cut(s) 142, 898, 958
BspANI GGCC 3 cut(s) 77, 537, 990
BspFNI CGCG 1 cut(s) 188
BspHI TCATGA 2 cut(s) 490, 496
BspPI GGATC 4 cut(s) 300, 560, 738, 892
BspQI GCTCTTC 1 cut(s) 956
BspT104I TTCGAA 1 cut(s) 860
BspTNI GGTCTC 2 cut(s) 315, 507
BsrDI GCAATG 1 cut(s) 643
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 2 cut(s) 660, 866
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 2 cut(s) 72, 991
BssMI GATC 8 cut(s) 292, 487, 493, 502, 565, 730, 884, 916
BssT1I CCWWGG 2 cut(s) 72, 991
Bst6I CTCTTC 1 cut(s) 956
BstAPI GCANNNNNTGC 1 cut(s) 107
BstBAI YACGTR 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 860
BstC8I GCNNGC 4 cut(s) 79, 181, 365, 535
BstDEI CTNAG 1 cut(s) 638
BstDSI CCRYGG 2 cut(s) 72, 991
BstFNI CGCG 1 cut(s) 188
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 8 cut(s) 295, 490, 496, 505, 568, 733, 887, 919
BstMAI GTCTC 3 cut(s) 315, 386, 507
BstMBI GATC 8 cut(s) 292, 487, 493, 502, 565, 730, 884, 916
BstMCI CGRYCG 1 cut(s) 902
BstMWI GCNNNNNNNGC 5 cut(s) 107, 148, 185, 352, 454
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 537
BstSFI CTRYAG 1 cut(s) 438
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 1 cut(s) 922
BstV2I GAAGAC 3 cut(s) 79, 336, 918
BstXI CCANNNNNNTGG 1 cut(s) 215
BsuRI GGCC 3 cut(s) 77, 537, 990
BtgI CCRYGG 2 cut(s) 72, 991
BtgZI GCGATG 1 cut(s) 537
BtrI CACGTC 1 cut(s) 396
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 2 cut(s) 591, 653
Cac8I GCNNGC 4 cut(s) 79, 181, 365, 535
CciI TCATGA 2 cut(s) 490, 496
CfoI GCGC 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 536
Csp6I GTAC 1 cut(s) 112
CviAII CATG 7 cut(s) 73, 289, 368, 384, 491, 497, 992
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 1 cut(s) 638
DpnI GATC 8 cut(s) 294, 489, 495, 504, 567, 732, 886, 918
DpnII GATC 8 cut(s) 292, 487, 493, 502, 565, 730, 884, 916
EaeI YGGCCR 2 cut(s) 75, 988
Eam1104I CTCTTC 1 cut(s) 956
EarI CTCTTC 1 cut(s) 956
Eco130I CCWWGG 2 cut(s) 72, 991
Eco31I GGTCTC 2 cut(s) 315, 507
Eco57I CTGAAG 2 cut(s) 18, 912
EcoT14I CCWWGG 2 cut(s) 72, 991
EcoT22I ATGCAT 1 cut(s) 389
ErhI CCWWGG 2 cut(s) 72, 991
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 7 cut(s) 76, 292, 371, 387, 494, 500, 995
FaqI GGGAC 1 cut(s) 28
FatI CATG 7 cut(s) 72, 288, 367, 383, 490, 496, 991
FbaI TGATCA 3 cut(s) 487, 493, 502
Fnu4HI GCNGC 1 cut(s) 911
Fsp4HI GCNGC 1 cut(s) 911
FspBI CTAG 3 cut(s) 98, 122, 326
GlaI GCGC 1 cut(s) 189
GluI GCNGC 1 cut(s) 911
HaeIII GGCC 3 cut(s) 77, 537, 990
HapII CCGG 3 cut(s) 78, 422, 539
HhaI GCGC 1 cut(s) 190
Hin1II CATG 7 cut(s) 76, 292, 371, 387, 494, 500, 995
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 3 cut(s) 16, 244, 250
HpaII CCGG 3 cut(s) 78, 422, 539
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 2 cut(s) 581, 940
Hpy188I TCNGA 3 cut(s) 249, 297, 847
Hpy188III TCNNGA 5 cut(s) 235, 491, 497, 563, 569
Hpy8I GTNNAC 2 cut(s) 581, 940
HpyAV CCTTC 2 cut(s) 667, 886
HpyCH4IV ACGT 4 cut(s) 257, 395, 470, 741
HpyCH4V TGCA 4 cut(s) 163, 169, 363, 387
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 148, 185, 352, 454
HpyF3I CTNAG 1 cut(s) 638
HpySE526I ACGT 4 cut(s) 257, 395, 470, 741
Hsp92II CATG 7 cut(s) 76, 292, 371, 387, 494, 500, 995
HspAI GCGC 1 cut(s) 188
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Ksp22I TGATCA 3 cut(s) 487, 493, 502
Kzo9I GATC 8 cut(s) 292, 487, 493, 502, 565, 730, 884, 916
LguI GCTCTTC 1 cut(s) 956
LmnI GCTCC 1 cut(s) 29
Lsp1109I GCAGC 1 cut(s) 922
LweI GCATC 2 cut(s) 150, 342
MaeI CTAG 3 cut(s) 98, 122, 326
MaeII ACGT 4 cut(s) 257, 395, 470, 741
MaeIII GTNAC 1 cut(s) 651
MalI GATC 8 cut(s) 294, 489, 495, 504, 567, 732, 886, 918
MboI GATC 8 cut(s) 292, 487, 493, 502, 565, 730, 884, 916
MlsI TGGCCA 1 cut(s) 990
MluCI AATT 7 cut(s) 170, 279, 415, 585, 614, 693, 877
MluNI TGGCCA 1 cut(s) 990
MlyI GAGTC 1 cut(s) 10
MmeI TCCRAC 1 cut(s) 690
MnlI CCTC 8 cut(s) 138, 141, 194, 565, 568, 570, 708, 900
Mox20I TGGCCA 1 cut(s) 990
Mph1103I ATGCAT 1 cut(s) 389
MroNI GCCGGC 1 cut(s) 77
MroXI GAANNNNTTC 2 cut(s) 282, 925
MscI TGGCCA 1 cut(s) 990
MseI TTAA 1 cut(s) 701
MslI CAYNNNNRTG 1 cut(s) 495
Msp20I TGGCCA 1 cut(s) 990
MspI CCGG 3 cut(s) 78, 422, 539
MspR9I CCNGG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 409
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 5 cut(s) 107, 148, 185, 352, 454
NaeI GCCGGC 1 cut(s) 79
NciI CCSGG 1 cut(s) 539
NcoI CCATGG 2 cut(s) 72, 991
NdeII GATC 8 cut(s) 292, 487, 493, 502, 565, 730, 884, 916
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 7 cut(s) 76, 292, 371, 387, 494, 500, 995
NmeAIII GCCGAG 1 cut(s) 381
NmuCI GTSAC 1 cut(s) 651
NsiI ATGCAT 1 cut(s) 389
NspI RCATGY 1 cut(s) 371
NspV TTCGAA 1 cut(s) 860
PagI TCATGA 2 cut(s) 490, 496
PciSI GCTCTTC 1 cut(s) 956
PctI GAATGC 1 cut(s) 409
PdiI GCCGGC 1 cut(s) 79
PdmI GAANNNNTTC 2 cut(s) 282, 925
PfeI GAWTC 2 cut(s) 244, 250
PkrI GCNGC 1 cut(s) 912
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
Ppu21I YACGTR 1 cut(s) 258
Psp1406I AACGTT 1 cut(s) 470
PspPI GGNCC 1 cut(s) 536
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 495
SapI GCTCTTC 1 cut(s) 956
SaqAI TTAA 1 cut(s) 701
SatI GCNGC 1 cut(s) 911
Sau3AI GATC 8 cut(s) 292, 487, 493, 502, 565, 730, 884, 916
Sau96I GGNCC 1 cut(s) 536
SchI GAGTC 1 cut(s) 10
ScrFI CCNGG 1 cut(s) 539
SfaNI GCATC 2 cut(s) 150, 342
SfcI CTRYAG 1 cut(s) 438
SfuI TTCGAA 1 cut(s) 860
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 7 cut(s) 170, 279, 415, 585, 614, 693, 877
SsiI CCGC 3 cut(s) 142, 898, 958
SspMI CTAG 3 cut(s) 98, 122, 326
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 2 cut(s) 72, 991
TaiI ACGT 4 cut(s) 260, 398, 473, 744
TaqI TCGA 4 cut(s) 525, 860, 902, 919
TasI AATT 7 cut(s) 170, 279, 415, 585, 614, 693, 877
TfiI GAWTC 2 cut(s) 244, 250
Tru1I TTAA 1 cut(s) 701
Tru9I TTAA 1 cut(s) 701
TscAI CASTG 2 cut(s) 598, 660
TseFI GTSAC 1 cut(s) 651
TseI GCWGC 1 cut(s) 910
Tsp45I GTSAC 1 cut(s) 651
TspDTI ATGAA 3 cut(s) 453, 686, 774
TspGWI ACGGA 1 cut(s) 995
TspRI CASTG 2 cut(s) 598, 660
XapI RAATTY 2 cut(s) 279, 693
XceI RCATGY 1 cut(s) 371
XmnI GAANNNNTTC 2 cut(s) 282, 925
XspI CTAG 3 cut(s) 98, 122, 326
Zsp2I ATGCAT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.