Rroxscaffold_6G00410150

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0016881
Physical Location & Seq
Reverse (-)
32572316 .. 32573854
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410150.1

Sequence Viewer

Length: 990 bp
ATGGAGGACATTACATCCAACGTCCAACCTTCTTCACGGTCCTACTTCACACCCCTTTTGATTTCCATGGCCGGAATAGTCTCCACTACTCTAGCCGTAATAGCATACCATTTCATAGTGACGAAGTTTTGCTTGAGGCATAGGCATAGGCAGAGACAGAGAATACTAGAATCATTCTCTAATTTCTCACCTCAACCGAACCCCAACAACAACCAAACTGGGGTAGATGAGAAAGTCCTGAGCTCTATCCCAATTCTCTCTTACTCCACAAAAGACGGACTGACCGGTCTAGATCAGAGCGAGTGTGTGATCTGCTTGGGTGAGCTGGAAGATGGTGACATGGTGAGGCTTCTGCCCAATTGCAAACATGTGTTTCACAATCCATGCATAGACAAATGGTTCTTGGCACATACAAACTGCCCGGTTTGTAGGGCACCTATAGCCGCGCCCATTAAAACTGTGGAGCCCTTGCCTACTGATCAAGAAACCCGAGATCAAGAAGTTCGAGATGAAGCCAGCAGCGTTGAATGTTCTAATTCGAATTCGAATTCGTCTCCCCAGAGTCATGAGGAGGAAAGGGGACTACTTAGCAATAATGCAACCACTGTTCAATTGTCAAAAAATGGTTTGCTAAGGAATTGTGTGTCCCTCGTGCTGTTGCCTATAGAAGGAAGTAGTACTAAGCAGCAGAAAAGATTTGTGAACGGGTTGGATAGGTCATTGTCTTTGGATCTGTCACATATCCTCATCAACGTACAGAGAGAATGTGGCGATGATCACAAGCCCTCTTCATCATCATCACCATCTTCTCCTTCTTCTTCTTCTCCTGCTCTGGCGAAAGCGGTTCTTGTAGAGTCTGGATCATTCAGGGAAAGATCTTTCAGACAGTTTGATCGGATGTCTACAGTGTTAATGAGATCACTTTCGCAGTTGCGGGTAGGCCAAAGTAGCAGCACCATGGCTAATGGTCATGAAATTCTTCCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

329

Amino Acids

36.27

Weight (kDa)

6.55

Isoelectric Point (pI)

65.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 101 - 144 2.6e-14 Ring finger domain
zf-RING_11 PF17123 101 - 130 1.7e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 101 - 143 3.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 101 - 144 3.3e-07 RING-H2 zinc finger domain
zf-RING_5 PF14634 101 - 144 4.6e-06 zinc-RING finger domain
zf-C3HC4 PF00097 102 - 143 1.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016881)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17171
malus_domestica MD12G1103100.v1.1 MD14G1097100.v1.1
prunus_persica Prupe.7G123200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471271
rosa_laevigata RLG00000024192
rosa_multiflora Rmu_ssc0000091.1_g000001
rosa_roxburghii Rroxscaffold_6G00410150
rosa_rugosa Rorug03G0116700
rosa_samantha Rh3AG166000 Rh3BG191400 Rh3CG181700 Rh3DG184300
rosa_wichuraiana Rw3G015620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 433
AccI GTMKAC 1 cut(s) 902
AccII CGCG 1 cut(s) 446
AciI CCGC 3 cut(s) 444, 842, 934
AclWI GGATC 2 cut(s) 738, 868
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 3 cut(s) 541, 547, 975
AfaI GTAC 2 cut(s) 679, 756
AfiI CCNNNNNNNGG 2 cut(s) 220, 668
AflIII ACRYGT 1 cut(s) 367
AgeI ACCGGT 1 cut(s) 284
AgsI TTSAA 2 cut(s) 527, 611
AluBI AGCT 2 cut(s) 243, 325
AluI AGCT 2 cut(s) 243, 325
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 3 cut(s) 85, 148, 558
AlwI GGATC 2 cut(s) 738, 868
Ama87I CYCGRG 1 cut(s) 489
AoxI GGCC 2 cut(s) 69, 940
ApeKI GCWGC 3 cut(s) 519, 685, 951
ApoI RAATTY 3 cut(s) 541, 547, 975
AsiGI ACCGGT 1 cut(s) 284
Asp700I GAANNNNTTC 1 cut(s) 978
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 1 cut(s) 39
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 5 cut(s) 180, 332, 347, 355, 792
AsuII TTCGAA 2 cut(s) 539, 545
AvaI CYCGRG 1 cut(s) 489
AvaII GGWCC 1 cut(s) 39
BaeGI GKGCMC 1 cut(s) 436
BanI GGYRCC 1 cut(s) 433
BanII GRGCYC 2 cut(s) 245, 468
BauI CACGAG 1 cut(s) 650
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 3 cut(s) 531, 697, 963
BccI CCATC 2 cut(s) 326, 811
BceAI ACGGC 1 cut(s) 80
BclI TGATCA 2 cut(s) 478, 775
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 3 cut(s) 85, 148, 558
BfaI CTAG 3 cut(s) 92, 167, 290
BfmI CTRYAG 3 cut(s) 438, 663, 903
BglII AGATCT 1 cut(s) 875
BisI GCNGC 4 cut(s) 444, 520, 686, 952
BlsI GCNGC 4 cut(s) 445, 521, 687, 953
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 1 cut(s) 422
Bme18I GGWCC 1 cut(s) 39
BmeT110I CYCGRG 1 cut(s) 489
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 2 cut(s) 435, 465
BmrFI CCNGG 1 cut(s) 422
BmrI ACTGGG 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 228
Bpu10I CCTNAGC 2 cut(s) 239, 632
Bpu14I TTCGAA 2 cut(s) 539, 545
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 66, 957
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 668
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 223
BseDI CCNNGG 2 cut(s) 66, 957
BseGI GGATG 2 cut(s) 14, 903
BseLI CCNNNNNNNGG 2 cut(s) 220, 668
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 223
BseRI GAGGAG 1 cut(s) 584
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 3 cut(s) 531, 697, 963
Bsh1236I CGCG 1 cut(s) 446
BshFI GGCC 2 cut(s) 71, 942
BshNI GGYRCC 1 cut(s) 433
BshTI ACCGGT 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 489
BsiSI CCGG 3 cut(s) 72, 285, 422
BslFI GGGAC 2 cut(s) 594, 631
BslI CCNNNNNNNGG 2 cut(s) 220, 668
BsmAI GTCTC 3 cut(s) 85, 148, 558
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 2 cut(s) 594, 631
BsnI GGCC 2 cut(s) 71, 942
BsoBI CYCGRG 1 cut(s) 489
Bsp119I TTCGAA 2 cut(s) 539, 545
Bsp1286I GDGCHC 3 cut(s) 245, 436, 468
Bsp19I CCATGG 2 cut(s) 66, 957
BspACI CCGC 3 cut(s) 444, 842, 934
BspANI GGCC 2 cut(s) 71, 942
BspCNI CTCAG 1 cut(s) 231
BspFNI CGCG 1 cut(s) 446
BspHI TCATGA 2 cut(s) 565, 970
BspLI GGNNCC 2 cut(s) 435, 465
BspPI GGATC 2 cut(s) 738, 868
BspT104I TTCGAA 2 cut(s) 539, 545
BspT107I GGYRCC 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 223
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 66, 957
BssSI CACGAG 1 cut(s) 650
BssT1I CCWWGG 2 cut(s) 66, 957
Bst2BI CACGAG 1 cut(s) 650
Bst4CI ACNGT 5 cut(s) 39, 460, 607, 888, 907
Bst6I CTCTTC 1 cut(s) 793
BstBI TTCGAA 2 cut(s) 539, 545
BstC8I GCNNGC 1 cut(s) 517
BstDEI CTNAG 4 cut(s) 239, 587, 632, 681
BstDSI CCRYGG 2 cut(s) 66, 957
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 2 cut(s) 14, 903
BstFNI CGCG 1 cut(s) 446
BstHHI GCGC 1 cut(s) 448
BstMAI GTCTC 3 cut(s) 85, 148, 558
BstMWI GCNNNNNNNGC 3 cut(s) 101, 440, 948
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 420
BstSFI CTRYAG 3 cut(s) 438, 663, 903
BstSLI GKGCMC 1 cut(s) 436
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 3 cut(s) 531, 697, 963
BstX2I RGATCY 2 cut(s) 730, 875
BstYI RGATCY 2 cut(s) 730, 875
BsuRI GGCC 2 cut(s) 71, 942
BtgI CCRYGG 2 cut(s) 66, 957
BtgZI GCGATG 1 cut(s) 786
BtsCI GGATG 2 cut(s) 14, 903
BtsIMutI CAGTG 2 cut(s) 603, 912
Cac8I GCNNGC 1 cut(s) 517
CciI TCATGA 2 cut(s) 565, 970
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 39
Csp6I GTAC 2 cut(s) 678, 755
CspAI ACCGGT 1 cut(s) 284
CviAII CATG 7 cut(s) 67, 340, 368, 384, 566, 958, 971
CviQI GTAC 2 cut(s) 678, 755
DdeI CTNAG 4 cut(s) 239, 587, 632, 681
DrdI GACNNNNNNGTC 1 cut(s) 285
DseDI GACNNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 793
EarI CTCTTC 1 cut(s) 793
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 66, 957
Eco24I GRGCYC 2 cut(s) 245, 468
Eco47I GGWCC 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 489
EcoICRI GAGCTC 1 cut(s) 243
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoRI GAATTC 2 cut(s) 541, 547
EcoT14I CCWWGG 2 cut(s) 66, 957
EcoT22I ATGCAT 1 cut(s) 389
EcoT38I GRGCYC 2 cut(s) 245, 468
ErhI CCWWGG 2 cut(s) 66, 957
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 7 cut(s) 70, 343, 371, 387, 569, 961, 974
FalI AAGNNNNNCTT 4 cut(s) 116, 148, 831, 863
FaqI GGGAC 2 cut(s) 594, 631
FatI CATG 7 cut(s) 66, 339, 367, 383, 565, 957, 970
FauI CCCGC 1 cut(s) 927
FbaI TGATCA 2 cut(s) 478, 775
FblI GTMKAC 1 cut(s) 902
Fnu4HI GCNGC 4 cut(s) 444, 520, 686, 952
FokI GGATG 1 cut(s) 910
FriOI GRGCYC 2 cut(s) 245, 468
Fsp4HI GCNGC 4 cut(s) 444, 520, 686, 952
FspBI CTAG 3 cut(s) 92, 167, 290
GlaI GCGC 1 cut(s) 447
GluI GCNGC 4 cut(s) 444, 520, 686, 952
HaeIII GGCC 2 cut(s) 71, 942
HapII CCGG 3 cut(s) 72, 285, 422
HhaI GCGC 1 cut(s) 448
Hin1II CATG 7 cut(s) 70, 343, 371, 387, 569, 961, 974
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HinfI GANTC 3 cut(s) 170, 562, 854
HpaII CCGG 3 cut(s) 72, 285, 422
HphI GGTGA 5 cut(s) 180, 332, 347, 355, 792
Hpy166II GTNNAC 2 cut(s) 703, 903
Hpy188I TCNGA 3 cut(s) 297, 884, 897
Hpy188III TCNNGA 8 cut(s) 238, 290, 482, 497, 506, 566, 858, 971
Hpy8I GTNNAC 2 cut(s) 703, 903
HpyAV CCTTC 3 cut(s) 39, 662, 822
HpyCH4III ACNGT 5 cut(s) 39, 460, 607, 888, 907
HpyCH4IV ACGT 2 cut(s) 21, 753
HpyCH4V TGCA 3 cut(s) 363, 387, 599
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 440, 948
HpyF3I CTNAG 4 cut(s) 239, 587, 632, 681
HpySE526I ACGT 2 cut(s) 21, 753
Hsp92II CATG 7 cut(s) 70, 343, 371, 387, 569, 961, 974
HspAI GCGC 1 cut(s) 446
Ksp22I TGATCA 2 cut(s) 478, 775
LmnI GCTCC 1 cut(s) 463
Lsp1109I GCAGC 3 cut(s) 531, 697, 963
MaeI CTAG 3 cut(s) 92, 167, 290
MaeII ACGT 2 cut(s) 21, 753
MaeIII GTNAC 3 cut(s) 118, 335, 735
MboII GAAGA 8 cut(s) 24, 341, 780, 798, 807, 810, 813, 971
MfeI CAATTG 2 cut(s) 358, 611
MflI RGATCY 2 cut(s) 730, 875
MhlI GDGCHC 3 cut(s) 245, 436, 468
MluCI AATT 9 cut(s) 181, 252, 358, 535, 541, 547, 611, 637, 975
MlyI GAGTC 2 cut(s) 571, 863
MmeI TCCRAC 3 cut(s) 42, 49, 690
MnlI CCTC 8 cut(s) 129, 201, 339, 562, 565, 659, 755, 796
Mph1103I ATGCAT 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 978
MseI TTAA 2 cut(s) 453, 911
MspI CCGG 3 cut(s) 72, 285, 422
MspR9I CCNGG 1 cut(s) 422
MunI CAATTG 2 cut(s) 358, 611
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 3 cut(s) 101, 440, 948
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 2 cut(s) 66, 957
NlaIII CATG 7 cut(s) 70, 343, 371, 387, 569, 961, 974
NlaIV GGNNCC 2 cut(s) 435, 465
NmuCI GTSAC 3 cut(s) 118, 335, 735
NsiI ATGCAT 1 cut(s) 389
NspI RCATGY 1 cut(s) 371
NspV TTCGAA 2 cut(s) 539, 545
PagI TCATGA 2 cut(s) 565, 970
PciI ACATGT 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 978
PfeI GAWTC 1 cut(s) 170
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 4 cut(s) 445, 521, 687, 953
PleI GAGTC 2 cut(s) 570, 862
PpsI GAGTC 2 cut(s) 570, 862
PscI ACATGT 1 cut(s) 367
Psp124BI GAGCTC 1 cut(s) 245
PspN4I GGNNCC 2 cut(s) 435, 465
PspPI GGNCC 1 cut(s) 39
PsuI RGATCY 2 cut(s) 730, 875
RsaI GTAC 2 cut(s) 679, 756
RsaNI GTAC 2 cut(s) 678, 755
SacI GAGCTC 1 cut(s) 245
SaqAI TTAA 2 cut(s) 453, 911
SatI GCNGC 4 cut(s) 444, 520, 686, 952
Sau96I GGNCC 1 cut(s) 39
ScaI AGTACT 1 cut(s) 679
SchI GAGTC 2 cut(s) 571, 863
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 3 cut(s) 245, 436, 468
SetI ASST 8 cut(s) 24, 31, 193, 245, 327, 439, 719, 756
SfcI CTRYAG 3 cut(s) 438, 663, 903
SfuI TTCGAA 2 cut(s) 539, 545
SinI GGWCC 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
Sse9I AATT 9 cut(s) 181, 252, 358, 535, 541, 547, 611, 637, 975
SsiI CCGC 3 cut(s) 444, 842, 934
SspMI CTAG 3 cut(s) 92, 167, 290
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 420
StyI CCWWGG 2 cut(s) 66, 957
TaaI ACNGT 5 cut(s) 39, 460, 607, 888, 907
TaiI ACGT 2 cut(s) 24, 756
TaqI TCGA 3 cut(s) 505, 539, 545
TasI AATT 9 cut(s) 181, 252, 358, 535, 541, 547, 611, 637, 975
TatI WGTACW 1 cut(s) 677
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 2 cut(s) 453, 911
Tru9I TTAA 2 cut(s) 453, 911
TscAI CASTG 2 cut(s) 610, 912
TseFI GTSAC 3 cut(s) 118, 335, 735
TseI GCWGC 3 cut(s) 519, 685, 951
Tsp45I GTSAC 3 cut(s) 118, 335, 735
TspDTI ATGAA 4 cut(s) 103, 525, 780, 987
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 2 cut(s) 610, 912
VpaK11BI GGWCC 1 cut(s) 39
XagI CCTNNNNNAGG 1 cut(s) 666
XapI RAATTY 3 cut(s) 541, 547, 975
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 1 cut(s) 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 902
XmnI GAANNNNTTC 1 cut(s) 978
XspI CTAG 3 cut(s) 92, 167, 290
ZrmI AGTACT 1 cut(s) 679
Zsp2I ATGCAT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.