Rh3DG184300

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0016881
Physical Location & Seq
Reverse (-)
16341542 .. 16342657
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG184300.1

Sequence Viewer

Length: 978 bp
ATGGAGGACGTTGCATCCAACTTCCAACCTTCTTCACGGTCCTACTTCACACCCCTTTTGATTTCCATGGCCGGAATAGTCTCCACTACTCTAGCCGTAATAGCATACCATTTCATAGTGACGAAGTTTTGCTTGAGGCATAGGCGTAGGCAGAGACAGAGAATACTAGAAGGATTCTCTAATTTCTCACCGCAACCGAACCCCAACAACAACCAAACTGGGGTAGATGAGAAAGTCCTGAGCTCTATCCCAATTCTCTCTTACTCCACAAAAGACGGACTGACCGGTCTAGATCAGAGCGAGTGTGTGATCTGCTTGGGCGAGCTGGAAGATGGTGACATGGTGAGGCTTCTGCCCAATTGCAAACATCTGTTTCACAATCCATGCATAGACAAATGGTTCTTGGCACATACAAACTGCCCGGTTTGTAGGGCACCTATAGCCGGGCCCATTAAAACTGTGGAGCCTTTGCCTACAGATCAAGAAACCCGAGATCAAGAAGTTCGAGATGAAGCCAGCAGCGTTGAATGTTCTAATTCGAATTCGTCTCCCCAGAGTCATGAGGAGGAAAGGGGACTACTTGGCAATAATGCAACCACTGTTCAATTGCCAAAAAATGGTTTGCTAAGGAATTGTGTGTCTCTCAAGGTGTTGCCTATAGAAGGAAGTAATGAGCAGAAAAGATTTGTGAACGGGTTGGAGAGGTCATTGTCTTTGGATCTGTCACATATCCTCATCAACGTACACAGAGAATGTGGTGATGATCACAAGCCCTCTTCATCATCATCACCATCTTCTCCTTCTTCTTCTTCTTCTCCTCTGGCGAAAGCGGTTCTTGTGGAGTGTAGATCATTCAGGGAAAGATCTTTCAGACAGTTTGATCGGATGTCTACAGTGTTAATAAGATCACTTTCGCAGTTGCGGGTGGGGCAAAGTAGCAGCACCATGGCTAATGGTCATGAAATTCTTCCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

325

Amino Acids

35.99

Weight (kDa)

6.79

Isoelectric Point (pI)

68.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 99 - 148 5.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 101 - 144 5e-14 Ring finger domain
zf-RING_11 PF17123 101 - 130 4.7e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 101 - 143 2.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 101 - 144 9e-07 RING-H2 zinc finger domain
zf-RING_5 PF14634 101 - 144 9.1e-06 zinc-RING finger domain
zf-C3HC4 PF00097 102 - 143 8.5e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016881)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17171
malus_domestica MD12G1103100.v1.1 MD14G1097100.v1.1
prunus_persica Prupe.7G123200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471271
rosa_laevigata RLG00000024192
rosa_multiflora Rmu_ssc0000091.1_g000001
rosa_roxburghii Rroxscaffold_6G00410150
rosa_rugosa Rorug03G0116700
rosa_samantha Rh3AG166000 Rh3BG191400 Rh3CG181700 Rh3DG184300
rosa_wichuraiana Rw3G015620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 433
AccB7I CCANNNNNTGG 1 cut(s) 617
AccI GTMKAC 1 cut(s) 890
AciI CCGC 3 cut(s) 191, 830, 922
AclWI GGATC 1 cut(s) 726
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 541, 963
AfaI GTAC 1 cut(s) 744
AfiI CCNNNNNNNGG 4 cut(s) 220, 443, 617, 662
AgeI ACCGGT 1 cut(s) 284
AgsI TTSAA 2 cut(s) 527, 605
AluBI AGCT 2 cut(s) 243, 325
AluI AGCT 2 cut(s) 243, 325
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 4 cut(s) 85, 148, 552, 645
AlwI GGATC 1 cut(s) 726
Ama87I CYCGRG 1 cut(s) 489
AoxI GGCC 2 cut(s) 69, 446
ApaI GGGCCC 1 cut(s) 450
ApeKI GCWGC 2 cut(s) 519, 939
ApoI RAATTY 2 cut(s) 541, 963
AsiGI ACCGGT 1 cut(s) 284
Asp700I GAANNNNTTC 1 cut(s) 966
AspS9I GGNCC 3 cut(s) 39, 446, 447
AsuC2I CCSGG 2 cut(s) 422, 445
AsuHPI GGTGA 5 cut(s) 180, 347, 355, 770, 780
AsuII TTCGAA 1 cut(s) 539
AvaI CYCGRG 1 cut(s) 489
AvaII GGWCC 1 cut(s) 39
BaeGI GKGCMC 2 cut(s) 436, 450
BanI GGYRCC 1 cut(s) 433
BanII GRGCYC 2 cut(s) 245, 450
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 531, 951
BccI CCATC 2 cut(s) 326, 799
BceAI ACGGC 1 cut(s) 80
BclI TGATCA 1 cut(s) 763
BcnI CCSGG 2 cut(s) 422, 445
BcoDI GTCTC 4 cut(s) 85, 148, 552, 645
BfaI CTAG 3 cut(s) 92, 167, 290
BfmI CTRYAG 4 cut(s) 438, 474, 657, 891
BglII AGATCT 1 cut(s) 863
BisI GCNGC 2 cut(s) 520, 940
BlsI GCNGC 2 cut(s) 521, 941
Bme1390I CCNGG 2 cut(s) 422, 445
Bme18I GGWCC 1 cut(s) 39
BmeT110I CYCGRG 1 cut(s) 489
BmgT120I GGNCC 3 cut(s) 39, 446, 447
BmiI GGNNCC 3 cut(s) 435, 448, 465
BmrFI CCNGG 2 cut(s) 422, 445
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 228
Bpu10I CCTNAGC 2 cut(s) 239, 626
Bpu14I TTCGAA 1 cut(s) 539
BpuEI CTTGAG 2 cut(s) 154, 629
BpuMI CCSGG 2 cut(s) 422, 445
BsaJI CCNNGG 2 cut(s) 66, 945
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 443, 617, 662
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 223
BseDI CCNNGG 2 cut(s) 66, 945
BseGI GGATG 2 cut(s) 14, 891
BseLI CCNNNNNNNGG 4 cut(s) 220, 443, 617, 662
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 223
BseRI GAGGAG 2 cut(s) 578, 807
BseSI GKGCMC 2 cut(s) 436, 450
BseXI GCAGC 2 cut(s) 531, 951
BshFI GGCC 2 cut(s) 71, 448
BshNI GGYRCC 1 cut(s) 433
BshTI ACCGGT 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 489
BsiSI CCGG 4 cut(s) 72, 285, 422, 444
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 4 cut(s) 220, 443, 617, 662
BsmAI GTCTC 4 cut(s) 85, 148, 552, 645
BsmBI CGTCTC 1 cut(s) 552
BsmFI GGGAC 1 cut(s) 588
BsnI GGCC 2 cut(s) 71, 448
BsoBI CYCGRG 1 cut(s) 489
Bsp119I TTCGAA 1 cut(s) 539
Bsp120I GGGCCC 1 cut(s) 446
Bsp1286I GDGCHC 3 cut(s) 245, 436, 450
Bsp19I CCATGG 2 cut(s) 66, 945
BspACI CCGC 3 cut(s) 191, 830, 922
BspANI GGCC 2 cut(s) 71, 448
BspCNI CTCAG 1 cut(s) 231
BspHI TCATGA 2 cut(s) 559, 958
BspLI GGNNCC 3 cut(s) 435, 448, 465
BspPI GGATC 1 cut(s) 726
BspT104I TTCGAA 1 cut(s) 539
BspT107I GGYRCC 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 223
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 66, 945
BssT1I CCWWGG 2 cut(s) 66, 945
Bst4CI ACNGT 5 cut(s) 39, 460, 601, 876, 895
Bst6I CTCTTC 1 cut(s) 781
BstBI TTCGAA 1 cut(s) 539
BstC8I GCNNGC 2 cut(s) 323, 517
BstDEI CTNAG 2 cut(s) 239, 626
BstDSI CCRYGG 2 cut(s) 66, 945
BstENI CCTNNNNNAGG 1 cut(s) 660
BstF5I GGATG 2 cut(s) 14, 891
BstMAI GTCTC 4 cut(s) 85, 148, 552, 645
BstMWI GCNNNNNNNGC 3 cut(s) 101, 440, 928
BstSCI CCNGG 2 cut(s) 420, 443
BstSFI CTRYAG 4 cut(s) 438, 474, 657, 891
BstSLI GKGCMC 2 cut(s) 436, 450
BstV1I GCAGC 2 cut(s) 531, 951
BstX2I RGATCY 2 cut(s) 718, 863
BstYI RGATCY 2 cut(s) 718, 863
BsuRI GGCC 2 cut(s) 71, 448
BtgI CCRYGG 2 cut(s) 66, 945
BtsCI GGATG 2 cut(s) 14, 891
BtsIMutI CAGTG 2 cut(s) 597, 900
Cac8I GCNNGC 2 cut(s) 323, 517
CciI TCATGA 2 cut(s) 559, 958
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 3 cut(s) 39, 446, 447
Csp6I GTAC 1 cut(s) 743
CspAI ACCGGT 1 cut(s) 284
CviAII CATG 6 cut(s) 67, 340, 384, 560, 946, 959
CviQI GTAC 1 cut(s) 743
DdeI CTNAG 2 cut(s) 239, 626
DrdI GACNNNNNNGTC 1 cut(s) 285
DseDI GACNNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 781
EarI CTCTTC 1 cut(s) 781
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 66, 945
Eco24I GRGCYC 2 cut(s) 245, 450
Eco47I GGWCC 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 489
EcoICRI GAGCTC 1 cut(s) 243
EcoNI CCTNNNNNAGG 1 cut(s) 660
EcoRI GAATTC 1 cut(s) 541
EcoT14I CCWWGG 2 cut(s) 66, 945
EcoT22I ATGCAT 1 cut(s) 389
EcoT38I GRGCYC 2 cut(s) 245, 450
ErhI CCWWGG 2 cut(s) 66, 945
Esp3I CGTCTC 1 cut(s) 552
FaeI CATG 6 cut(s) 70, 343, 387, 563, 949, 962
FalI AAGNNNNNCTT 4 cut(s) 116, 148, 819, 851
FaqI GGGAC 1 cut(s) 588
FatI CATG 6 cut(s) 66, 339, 383, 559, 945, 958
FauI CCCGC 1 cut(s) 915
FbaI TGATCA 1 cut(s) 763
FblI GTMKAC 1 cut(s) 890
Fnu4HI GCNGC 2 cut(s) 520, 940
FokI GGATG 1 cut(s) 898
FriOI GRGCYC 2 cut(s) 245, 450
Fsp4HI GCNGC 2 cut(s) 520, 940
FspBI CTAG 3 cut(s) 92, 167, 290
GluI GCNGC 2 cut(s) 520, 940
HaeIII GGCC 2 cut(s) 71, 448
HapII CCGG 4 cut(s) 72, 285, 422, 444
Hin1II CATG 6 cut(s) 70, 343, 387, 563, 949, 962
HinfI GANTC 2 cut(s) 174, 556
HpaII CCGG 4 cut(s) 72, 285, 422, 444
HphI GGTGA 5 cut(s) 180, 347, 355, 770, 780
Hpy166II GTNNAC 3 cut(s) 691, 745, 891
Hpy188I TCNGA 3 cut(s) 297, 872, 885
Hpy188III TCNNGA 7 cut(s) 238, 290, 482, 497, 506, 560, 959
Hpy8I GTNNAC 3 cut(s) 691, 745, 891
HpyAV CCTTC 4 cut(s) 39, 164, 656, 810
HpyCH4III ACNGT 5 cut(s) 39, 460, 601, 876, 895
HpyCH4IV ACGT 2 cut(s) 9, 741
HpyCH4V TGCA 4 cut(s) 14, 363, 387, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 440, 928
HpyF3I CTNAG 2 cut(s) 239, 626
HpySE526I ACGT 2 cut(s) 9, 741
Hsp92II CATG 6 cut(s) 70, 343, 387, 563, 949, 962
Ksp22I TGATCA 1 cut(s) 763
LmnI GCTCC 1 cut(s) 463
Lsp1109I GCAGC 2 cut(s) 531, 951
LweI GCATC 1 cut(s) 23
MaeI CTAG 3 cut(s) 92, 167, 290
MaeII ACGT 2 cut(s) 9, 741
MaeIII GTNAC 3 cut(s) 118, 335, 723
MboII GAAGA 9 cut(s) 24, 341, 768, 786, 795, 798, 801, 804, 959
MfeI CAATTG 2 cut(s) 358, 605
MflI RGATCY 2 cut(s) 718, 863
MhlI GDGCHC 3 cut(s) 245, 436, 450
MluCI AATT 8 cut(s) 181, 252, 358, 535, 541, 605, 631, 963
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 3 cut(s) 42, 49, 678
MnlI CCTC 8 cut(s) 129, 339, 556, 559, 696, 743, 784, 828
Mph1103I ATGCAT 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 966
MseI TTAA 2 cut(s) 453, 899
MspI CCGG 4 cut(s) 72, 285, 422, 444
MspR9I CCNGG 2 cut(s) 422, 445
MunI CAATTG 2 cut(s) 358, 605
MwoI GCNNNNNNNGC 3 cut(s) 101, 440, 928
NciI CCSGG 2 cut(s) 422, 445
NcoI CCATGG 2 cut(s) 66, 945
NlaIII CATG 6 cut(s) 70, 343, 387, 563, 949, 962
NlaIV GGNNCC 3 cut(s) 435, 448, 465
NmuCI GTSAC 3 cut(s) 118, 335, 723
NsiI ATGCAT 1 cut(s) 389
NspV TTCGAA 1 cut(s) 539
PagI TCATGA 2 cut(s) 559, 958
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 1 cut(s) 174
PflMI CCANNNNNTGG 1 cut(s) 617
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 2 cut(s) 521, 941
PleI GAGTC 1 cut(s) 564
PpsI GAGTC 1 cut(s) 564
Psp124BI GAGCTC 1 cut(s) 245
PspN4I GGNNCC 3 cut(s) 435, 448, 465
PspOMI GGGCCC 1 cut(s) 446
PspPI GGNCC 3 cut(s) 39, 446, 447
PsuI RGATCY 2 cut(s) 718, 863
RsaI GTAC 1 cut(s) 744
RsaNI GTAC 1 cut(s) 743
SacI GAGCTC 1 cut(s) 245
SaqAI TTAA 2 cut(s) 453, 899
SatI GCNGC 2 cut(s) 520, 940
Sau96I GGNCC 3 cut(s) 39, 446, 447
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 2 cut(s) 422, 445
SduI GDGCHC 3 cut(s) 245, 436, 450
SetI ASST 8 cut(s) 12, 31, 245, 327, 439, 651, 707, 744
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 4 cut(s) 438, 474, 657, 891
SfuI TTCGAA 1 cut(s) 539
SinI GGWCC 1 cut(s) 39
SmlI CTYRAG 2 cut(s) 133, 644
SmoI CTYRAG 2 cut(s) 133, 644
Sse9I AATT 8 cut(s) 181, 252, 358, 535, 541, 605, 631, 963
SsiI CCGC 3 cut(s) 191, 830, 922
SspMI CTAG 3 cut(s) 92, 167, 290
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 2 cut(s) 420, 443
StyI CCWWGG 2 cut(s) 66, 945
TaaI ACNGT 5 cut(s) 39, 460, 601, 876, 895
TaiI ACGT 2 cut(s) 12, 744
TaqI TCGA 2 cut(s) 505, 539
TasI AATT 8 cut(s) 181, 252, 358, 535, 541, 605, 631, 963
TfiI GAWTC 1 cut(s) 174
Tru1I TTAA 2 cut(s) 453, 899
Tru9I TTAA 2 cut(s) 453, 899
TscAI CASTG 2 cut(s) 604, 900
TseFI GTSAC 3 cut(s) 118, 335, 723
TseI GCWGC 2 cut(s) 519, 939
Tsp45I GTSAC 3 cut(s) 118, 335, 723
TspDTI ATGAA 4 cut(s) 103, 525, 768, 975
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 2 cut(s) 604, 900
Van91I CCANNNNNTGG 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 39
XagI CCTNNNNNAGG 1 cut(s) 660
XapI RAATTY 2 cut(s) 541, 963
XbaI TCTAGA 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 890
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 3 cut(s) 92, 167, 290
Zsp2I ATGCAT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.