FvH4_6g18411

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010421
Physical Location & Seq
Reverse (-)
12164896 .. 12168333
3438 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18411.t1

Sequence Viewer

Length: 1317 bp
ATGATGAAATTGTGTCCGAATCTGTTCTCTCTACTCCTAACCATCTCTCTCTTCCCTCAACTCCTGAAAGCGAAAGATGATGATTTTGTTAGGCAACCTCCGCGGAGAGTAATATTCACCCACCATGACCGTTCCGATTCGGACCCTCAACAGGTGCATATTTCACTGGTGGGAAAAGATCACATGAAAGTTTCATGGGTGACAGACAGCAAACATAGCAGTTCTCTTGTTGAGTACGGAAAACAATCTGGAAAATACAATGCAAAAGCTAATGGAGAACACACATCCTACGAATACTTCTTTTATAGTTCTGGGAAGATCCACCATGTTACCATTGGCCCTTTGGAGCCATCAACTACATACTTCTACAGGTGTGGAGGTTCCGGCCCGGAGTTCTCCTTCAAGACACCCCCTTCAACACTTCCTCTTGATTTTGTAGTCGTTGGTGACCTAGGGCAGACTGAATGGACTAACTCCACCCTCGACCACATCCAAAGTATAGACTATGACGTCCTCCTGCTACCGGGGGATCTATCTTACGCCGATACCCACCAGCCCCTTTGGGACTCATTCGGCCGCATTGTAGAACCCTATGCCAGCCGCCGGCCATGGATGGTCACAGAAGGCAACCACGAAATCGAAATCTTCCCCATCATCTACCCAACCGGTTTTAAAGCCTACAATGCAAGGTGGCCGATGCCCTACCAAGAGAGCGGATCCACCTCAAACTTGTACTACTCCTTCGAAGTTGCCGGCACTCACGTCGTCATGCTTGGTTCCTACGCGGAGTTTAAGGCTGGTTCAGACCAGTACCAGTGGCTTCAGGCTGATTTAGCAAAGATTGACCGGAAAAAGACGCCGTGGGTGGTTGCACTTCTGCATGCACCGTGGTATAATACCAACACTGCTCATCAAGGTGAAGGAGACAGCATGAGGTTAGCCATGGAAGAGCTGCTGTATAAGGCACAGGTTGATTTGGTTTTTCAAGGCCATGTTCATGCGTATGAACGATTTACCAGGGTTTATAACAACAAGGCTGACCCATGTGGTCCGATTTATATAACCATTGGCGATGGTGGTAACCGAGAAGGACTAGCATTATCGTTCGAAAAGCCTACTTCCCCTCTCTCCTTGTTCCGAGAAGCGAGCTTCGGACATGGAAGGCTAAAAATGGTGAATGAAACACATGCATTGTGGGGATGGCATCGGAATAACGACTCAAACTTCATTGTGAAAGACCAGGTCTGGTTACAAAGTTTGAGCTCCTCTAAAACTTGCTCCTCTAAAACTTCACCATCCCGTAAAGATGAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

439

Amino Acids

49.76

Weight (kDa)

6.34

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 49 - 136 2e-24 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 145 - 335 6.9e-19 Calcineurin-like phosphoesterase
Metallophos_C PF14008 350 - 408 2.5e-18 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1026
AasI GACNNNNNNGTC 1 cut(s) 509
AatII GACGTC 1 cut(s) 513
AccBSI CCGCTC 1 cut(s) 714
AccII CGCG 2 cut(s) 103, 785
AciI CCGC 6 cut(s) 101, 103, 577, 601, 714, 785
AclWI GGATC 4 cut(s) 313, 537, 711, 724
AcoI YGGCCR 3 cut(s) 574, 605, 692
AcuI CTGAAG 1 cut(s) 806
AcyI GRCGYC 2 cut(s) 510, 857
AfaI GTAC 3 cut(s) 236, 734, 812
AfiI CCNNNNNNNGG 3 cut(s) 151, 523, 603
AgeI ACCGGT 1 cut(s) 665
AgsI TTSAA 3 cut(s) 403, 417, 986
AjiI CACGTC 1 cut(s) 763
AjnI CCWGG 2 cut(s) 1016, 1239
AluBI AGCT 4 cut(s) 269, 952, 1149, 1263
AluI AGCT 4 cut(s) 269, 952, 1149, 1263
Alw21I GWGCWC 1 cut(s) 1265
Alw26I GTCTC 1 cut(s) 918
AlwI GGATC 4 cut(s) 313, 537, 711, 724
AoxI GGCC 6 cut(s) 337, 385, 574, 605, 692, 988
ApeKI GCWGC 1 cut(s) 952
AsiGI ACCGGT 1 cut(s) 665
Asp700I GAANNNNTTC 1 cut(s) 23
AspA2I CCTAGG 1 cut(s) 451
AspS9I GGNCC 4 cut(s) 142, 338, 386, 1049
AsuC2I CCSGG 2 cut(s) 389, 525
AsuHPI GGTGA 6 cut(s) 109, 211, 458, 929, 1186, 1284
AsuII TTCGAA 2 cut(s) 744, 1107
AvaII GGWCC 2 cut(s) 142, 1049
AvrII CCTAGG 1 cut(s) 451
BamHI GGATCC 1 cut(s) 716
BanII GRGCYC 1 cut(s) 1265
BarI GAAGNNNNNNTAC 2 cut(s) 725, 757
Bbv12I GWGCWC 1 cut(s) 1265
BbvI GCAGC 1 cut(s) 939
BccI CCATC 7 cut(s) 50, 358, 607, 659, 1067, 1194, 1303
BceAI ACGGC 1 cut(s) 844
BcgI CGANNNNNNTGC 2 cut(s) 745, 779
BciT130I CCWGG 2 cut(s) 1018, 1241
BcnI CCSGG 2 cut(s) 389, 525
BcoDI GTCTC 1 cut(s) 918
BfaI CTAG 2 cut(s) 452, 1094
BfmI CTRYAG 1 cut(s) 367
BisI GCNGC 3 cut(s) 577, 601, 953
BlnI CCTAGG 1 cut(s) 451
BlsI GCNGC 3 cut(s) 578, 602, 954
Bme1390I CCNGG 4 cut(s) 389, 525, 1018, 1241
Bme18I GGWCC 2 cut(s) 142, 1049
BmgBI CACGTC 1 cut(s) 763
BmgT120I GGNCC 4 cut(s) 142, 338, 386, 1049
BmiI GGNNCC 5 cut(s) 144, 348, 382, 718, 778
BmrFI CCNGG 4 cut(s) 389, 525, 1018, 1241
BmsI GCATC 2 cut(s) 687, 1213
Bpu14I TTCGAA 2 cut(s) 744, 1107
BpuMI CCSGG 2 cut(s) 389, 525
BsaHI GRCGYC 2 cut(s) 510, 857
BsaJI CCNNGG 8 cut(s) 101, 451, 524, 608, 860, 887, 942, 1017
BsaWI WCCGGW 2 cut(s) 665, 846
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 523, 603
Bse118I RCCGGY 3 cut(s) 603, 665, 752
Bse1I ACTGG 3 cut(s) 171, 808, 814
BseBI CCWGG 2 cut(s) 1018, 1241
BseDI CCNNGG 8 cut(s) 101, 451, 524, 608, 860, 887, 942, 1017
BseGI GGATG 5 cut(s) 284, 489, 618, 1205, 1295
BseLI CCNNNNNNNGG 3 cut(s) 151, 523, 603
BseNI ACTGG 3 cut(s) 171, 808, 814
BseRI GAGGAG 2 cut(s) 1255, 1270
BseX3I CGGCCG 1 cut(s) 574
BseXI GCAGC 1 cut(s) 939
Bsh1236I CGCG 2 cut(s) 103, 785
Bsh1285I CGRYCG 1 cut(s) 577
BshFI GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
BshTI ACCGGT 1 cut(s) 665
BsiEI CGRYCG 1 cut(s) 577
BsiHKAI GWGCWC 1 cut(s) 1265
BsiSI CCGG 7 cut(s) 384, 389, 524, 604, 666, 753, 847
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 3 cut(s) 151, 523, 603
BsmAI GTCTC 1 cut(s) 918
BsmFI GGGAC 1 cut(s) 578
BsnI GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
Bsp119I TTCGAA 2 cut(s) 744, 1107
Bsp1286I GDGCHC 1 cut(s) 1265
Bsp143I GATC 4 cut(s) 178, 318, 529, 716
Bsp19I CCATGG 2 cut(s) 608, 942
BspACI CCGC 6 cut(s) 101, 103, 577, 601, 714, 785
BspANI GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
BspFNI CGCG 2 cut(s) 103, 785
BspLI GGNNCC 5 cut(s) 144, 348, 382, 718, 778
BspPI GGATC 4 cut(s) 313, 537, 711, 724
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 2 cut(s) 744, 1107
BsrBI CCGCTC 1 cut(s) 714
BsrFI RCCGGY 3 cut(s) 603, 665, 752
BsrI ACTGG 3 cut(s) 171, 808, 814
BssAI RCCGGY 3 cut(s) 603, 665, 752
BssECI CCNNGG 8 cut(s) 101, 451, 524, 608, 860, 887, 942, 1017
BssMI GATC 4 cut(s) 178, 318, 529, 716
BssNI GRCGYC 2 cut(s) 510, 857
BssT1I CCWWGG 3 cut(s) 451, 608, 942
Bst2UI CCWGG 2 cut(s) 1018, 1241
Bst4CI ACNGT 2 cut(s) 131, 888
Bst6I CTCTTC 2 cut(s) 56, 942
BstACI GRCGYC 2 cut(s) 510, 857
BstBI TTCGAA 2 cut(s) 744, 1107
BstC8I GCNNGC 5 cut(s) 598, 605, 754, 882, 1147
BstDSI CCRYGG 5 cut(s) 101, 608, 860, 887, 942
BstEII GGTNACC 2 cut(s) 446, 1079
BstF5I GGATG 5 cut(s) 284, 489, 618, 1205, 1295
BstFNI CGCG 2 cut(s) 103, 785
BstKTI GATC 4 cut(s) 181, 321, 532, 719
BstMAI GTCTC 1 cut(s) 918
BstMBI GATC 4 cut(s) 178, 318, 529, 716
BstMCI CGRYCG 1 cut(s) 577
BstMWI GCNNNNNNNGC 4 cut(s) 100, 216, 683, 833
BstNI CCWGG 2 cut(s) 1018, 1241
BstNSI RCATGY 2 cut(s) 884, 1190
BstPI GGTNACC 2 cut(s) 446, 1079
BstSCI CCNGG 4 cut(s) 387, 523, 1016, 1239
BstSFI CTRYAG 1 cut(s) 367
BstUI CGCG 2 cut(s) 103, 785
BstV1I GCAGC 1 cut(s) 939
BstX2I RGATCY 3 cut(s) 318, 529, 716
BstYI RGATCY 3 cut(s) 318, 529, 716
BstZI CGGCCG 1 cut(s) 574
BsuRI GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
BtgI CCRYGG 5 cut(s) 101, 608, 860, 887, 942
BtgZI GCGATG 1 cut(s) 1086
BtrI CACGTC 1 cut(s) 763
BtsCI GGATG 5 cut(s) 284, 489, 618, 1205, 1295
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 3 cut(s) 164, 821, 903
Cac8I GCNNGC 5 cut(s) 598, 605, 754, 882, 1147
Cfr10I RCCGGY 3 cut(s) 603, 665, 752
Cfr13I GGNCC 4 cut(s) 142, 338, 386, 1049
Cfr42I CCGCGG 1 cut(s) 104
CseI GACGC 1 cut(s) 865
CsiI ACCWGGT 1 cut(s) 1239
Csp6I GTAC 3 cut(s) 235, 733, 811
CspAI ACCGGT 1 cut(s) 665
CviQI GTAC 3 cut(s) 235, 733, 811
DpnI GATC 4 cut(s) 180, 320, 531, 718
DpnII GATC 4 cut(s) 178, 318, 529, 716
DraI TTTAAA 1 cut(s) 673
DrdI GACNNNNNNGTC 1 cut(s) 509
DseDI GACNNNNNNGTC 1 cut(s) 509
EaeI YGGCCR 3 cut(s) 574, 605, 692
EagI CGGCCG 1 cut(s) 574
Eam1104I CTCTTC 2 cut(s) 56, 942
EarI CTCTTC 2 cut(s) 56, 942
Ecl136II GAGCTC 1 cut(s) 1263
EclXI CGGCCG 1 cut(s) 574
Eco130I CCWWGG 3 cut(s) 451, 608, 942
Eco24I GRGCYC 1 cut(s) 1265
Eco47I GGWCC 2 cut(s) 142, 1049
Eco52I CGGCCG 1 cut(s) 574
Eco53kI GAGCTC 1 cut(s) 1263
Eco57I CTGAAG 1 cut(s) 806
Eco91I GGTNACC 2 cut(s) 446, 1079
EcoICRI GAGCTC 1 cut(s) 1263
EcoO65I GGTNACC 2 cut(s) 446, 1079
EcoRII CCWGG 2 cut(s) 1016, 1239
EcoT14I CCWWGG 3 cut(s) 451, 608, 942
EcoT22I ATGCAT 1 cut(s) 1192
EcoT38I GRGCYC 1 cut(s) 1265
ErhI CCWWGG 3 cut(s) 451, 608, 942
FaqI GGGAC 1 cut(s) 578
Fnu4HI GCNGC 3 cut(s) 577, 601, 953
FokI GGATG 5 cut(s) 271, 476, 625, 1212, 1282
FriOI GRGCYC 1 cut(s) 1265
Fsp4HI GCNGC 3 cut(s) 577, 601, 953
FspBI CTAG 2 cut(s) 452, 1094
GluI GCNGC 3 cut(s) 577, 601, 953
HaeIII GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
HapII CCGG 7 cut(s) 384, 389, 524, 604, 666, 753, 847
HgaI GACGC 1 cut(s) 865
Hin1I GRCGYC 2 cut(s) 510, 857
HinfI GANTC 4 cut(s) 19, 137, 566, 1217
HpaII CCGG 7 cut(s) 384, 389, 524, 604, 666, 753, 847
HphI GGTGA 6 cut(s) 109, 211, 458, 929, 1186, 1284
Hpy188I TCNGA 8 cut(s) 18, 136, 142, 805, 1053, 1139, 1154, 1209
Hpy188III TCNNGA 4 cut(s) 64, 249, 403, 428
Hpy99I CGWCG 1 cut(s) 767
HpyAV CCTTC 7 cut(s) 409, 423, 617, 751, 914, 1082, 1155
HpyCH4III ACNGT 2 cut(s) 131, 888
HpyCH4IV ACGT 2 cut(s) 510, 762
HpyCH4V TGCA 7 cut(s) 157, 263, 686, 872, 880, 884, 1190
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 216, 683, 833
HpySE526I ACGT 2 cut(s) 510, 762
Hsp92I GRCGYC 2 cut(s) 510, 857
KroI GCCGGC 2 cut(s) 603, 752
KroNI GCCGGC 2 cut(s) 605, 754
KspI CCGCGG 1 cut(s) 104
Kzo9I GATC 4 cut(s) 178, 318, 529, 716
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 3 cut(s) 346, 1268, 1283
Lsp1109I GCAGC 1 cut(s) 939
LweI GCATC 2 cut(s) 687, 1213
MabI ACCWGGT 1 cut(s) 1239
MaeI CTAG 2 cut(s) 452, 1094
MaeII ACGT 2 cut(s) 510, 762
MaeIII GTNAC 6 cut(s) 199, 328, 446, 616, 1079, 1248
MalI GATC 4 cut(s) 180, 320, 531, 718
MbiI CCGCTC 1 cut(s) 714
MboI GATC 4 cut(s) 178, 318, 529, 716
MboII GAAGA 4 cut(s) 43, 328, 637, 959
MflI RGATCY 3 cut(s) 318, 529, 716
MhlI GDGCHC 1 cut(s) 1265
MluCI AATT 2 cut(s) 8, 1310
MlyI GAGTC 2 cut(s) 560, 1211
Mph1103I ATGCAT 1 cut(s) 1192
MroNI GCCGGC 2 cut(s) 603, 752
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 2 cut(s) 672, 792
MslI CAYNNNNRTG 3 cut(s) 915, 996, 1002
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 7 cut(s) 384, 389, 524, 604, 666, 753, 847
MspR9I CCNGG 4 cut(s) 389, 525, 1018, 1241
MvaI CCWGG 2 cut(s) 1018, 1241
MvnI CGCG 2 cut(s) 103, 785
MwoI GCNNNNNNNGC 4 cut(s) 100, 216, 683, 833
NaeI GCCGGC 2 cut(s) 605, 754
NciI CCSGG 2 cut(s) 389, 525
NcoI CCATGG 2 cut(s) 608, 942
NdeII GATC 4 cut(s) 178, 318, 529, 716
NgoMIV GCCGGC 2 cut(s) 603, 752
NlaIV GGNNCC 5 cut(s) 144, 348, 382, 718, 778
NmuCI GTSAC 3 cut(s) 199, 446, 616
NsiI ATGCAT 1 cut(s) 1192
NspI RCATGY 2 cut(s) 884, 1190
NspV TTCGAA 2 cut(s) 744, 1107
PaeI GCATGC 1 cut(s) 884
PciSI GCTCTTC 1 cut(s) 942
PdiI GCCGGC 2 cut(s) 605, 754
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 2 cut(s) 19, 137
PflFI GACNNNGTC 1 cut(s) 1241
PinAI ACCGGT 1 cut(s) 665
PkrI GCNGC 3 cut(s) 578, 602, 954
PleI GAGTC 2 cut(s) 560, 1211
PpsI GAGTC 2 cut(s) 560, 1211
PsiI TTATAA 1 cut(s) 1026
Psp124BI GAGCTC 1 cut(s) 1265
Psp6I CCWGG 2 cut(s) 1016, 1239
PspEI GGTNACC 2 cut(s) 446, 1079
PspGI CCWGG 2 cut(s) 1016, 1239
PspN4I GGNNCC 5 cut(s) 144, 348, 382, 718, 778
PspPI GGNCC 4 cut(s) 142, 338, 386, 1049
PsuI RGATCY 3 cut(s) 318, 529, 716
PsyI GACNNNGTC 1 cut(s) 1241
RsaI GTAC 3 cut(s) 236, 734, 812
RsaNI GTAC 3 cut(s) 235, 733, 811
RseI CAYNNNNRTG 3 cut(s) 915, 996, 1002
SacI GAGCTC 1 cut(s) 1265
SacII CCGCGG 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 2 cut(s) 672, 792
SatI GCNGC 3 cut(s) 577, 601, 953
Sau3AI GATC 4 cut(s) 178, 318, 529, 716
Sau96I GGNCC 4 cut(s) 142, 338, 386, 1049
SchI GAGTC 2 cut(s) 560, 1211
ScrFI CCNGG 4 cut(s) 389, 525, 1018, 1241
SduI GDGCHC 1 cut(s) 1265
SexAI ACCWGGT 1 cut(s) 1239
SfaNI GCATC 2 cut(s) 687, 1213
SfcI CTRYAG 1 cut(s) 367
Sfr303I CCGCGG 1 cut(s) 104
SfuI TTCGAA 2 cut(s) 744, 1107
SgrBI CCGCGG 1 cut(s) 104
SinI GGWCC 2 cut(s) 142, 1049
SmiMI CAYNNNNRTG 3 cut(s) 915, 996, 1002
SphI GCATGC 1 cut(s) 884
Sse9I AATT 2 cut(s) 8, 1310
SsiI CCGC 6 cut(s) 101, 103, 577, 601, 714, 785
SspI AATATT 1 cut(s) 114
SspMI CTAG 2 cut(s) 452, 1094
SstI GAGCTC 1 cut(s) 1265
StyD4I CCNGG 4 cut(s) 387, 523, 1016, 1239
StyI CCWWGG 3 cut(s) 451, 608, 942
TaaI ACNGT 2 cut(s) 131, 888
TaiI ACGT 2 cut(s) 513, 765
TaqI TCGA 4 cut(s) 483, 639, 744, 1107
TasI AATT 2 cut(s) 8, 1310
TatI WGTACW 1 cut(s) 732
TauI GCSGC 2 cut(s) 579, 603
TfiI GAWTC 2 cut(s) 19, 137
Tru1I TTAA 2 cut(s) 672, 792
Tru9I TTAA 2 cut(s) 672, 792
TscAI CASTG 3 cut(s) 171, 821, 910
TseFI GTSAC 3 cut(s) 199, 446, 616
TseI GCWGC 1 cut(s) 952
Tsp45I GTSAC 3 cut(s) 199, 446, 616
TspDTI ATGAA 7 cut(s) 20, 183, 200, 986, 1020, 1194, 1216
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 3 cut(s) 171, 821, 910
Tth111I GACNNNGTC 1 cut(s) 1241
VpaK11BI GGWCC 2 cut(s) 142, 1049
XceI RCATGY 2 cut(s) 884, 1190
XcmI CCANNNNNNNNNTGG 2 cut(s) 332, 340
XmaJI CCTAGG 1 cut(s) 451
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 2 cut(s) 452, 1094
ZraI GACGTC 1 cut(s) 511
Zsp2I ATGCAT 1 cut(s) 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.