Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010421

Physical Location & Seq

Genomic Coordinates Reverse (-)

2334733 .. 2339497

Gene Structure 4765 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1023300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1332 bp

ATGGAGAAATTATATCTTCATGTGTTCTCTCTCCTCCTAGTCATCTGTCTCTTCCCTCAACTCATAAAATCACAAGATGATGATTATGTTCGACAACCTCCTCGCGGAGTAATATTCACCCACCACACTAGTTCGGATGAGGACCCTCAACAGGTGCATATTTCACTGGTGGGAAAAGATCATATGAGAGTTTCATGGGTTACTGAAAGCAAGCATGTTAAATCAATTGTGGAGTATGGAAAGGAATCTGGGATGTATAATGGGAAGGCAACTGGAGAACACACATCATACAAGTACTTTCTCTACAGCTCTGGGAAGATCCACCATGTTACCATTGGTCCCTTGGAACCTGCCACTACATACTTCTATAGGTGTGGAGGTTCAGGCCCCGAGTTCTCCTTCAAGACACCCCCACAAAAACTTCCCCTTGAATTTGCAGTGGCCGGTGACCTAGGTCAGACTGAATGGACTAACTCAACCCTACAACACATCGGAAGCATGGACTACGATGTGCTTCTCCTCCCCGGTGACTTGTCATACGCCGATACACAGCAACCGCTTTGGGACTCCTTCGGCCGCATTGTCGAGCCCTACGCCAGCAAACGCCCTTGGATGGTCACAGAAGGCAACCATGAAGTCGAGAGCATCCCAATCATCAACCCTACTGGCTTCAAGGCCTACAATGCCAGGTGGCCGATGCCCTACCAAGAGAGTGGGTCCACCTCAAACTTGTACTACTCTTTTGAAGTTGCCGGGACCCACGTCATCATGCTGGGATCCTATGCTGATTTTGATGCTAAGTCAGATCAGTACAAGTGGCTTCAGGCTGATTTGGCAAAGATTGATAGGAAGGTGACGCCGTGGATTGTTGTGCTTCTGCATGCACCGTGGTATAATACCAATTCTGCCCACAAAGGTGAAGGGGAGAGTATGAGGAAAGCAATGGAAGAGTTGCTGTACAATGCAAGGGTGGATGTGGTTTTTGCGGGGCATGTTCATGCGTATGAACGATTTACTAGGGTTTACAACAATGAAGCTGATCCATGTGGTCCAGTGTATATGACCATTGGTGACGGTGGAAATCGCGAAGGACTAGCATTAAAGTTCGAAAATCCGGCTTCCCCTCTGTCTTTGTACAGAGAGCCGAGTTTTGGACACGGAAGGCTCAGAGTATTAAACGAAACACATGCATTTTGGGGATGGCATCGAAACAACGAATCAAACTCCGTTGTAAAAGACCAGGTCTGGTTAGAAAGTTTGAGCAGCTCCAAAACATGCATGAAATCTGGGAGCCAGAAGGTGGGTTCATCATCTGTTAACGATGAATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

444

Amino Acids

50.07

Weight (kDa)

6.02

Isoelectric Point (pI)

33.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	49 - 136	2.7e-24	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	145 - 335	2.6e-18	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	350 - 413	1.9e-17	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010421)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52820
fragaria_vesca	FvH4_6g18411 FvH4_6g18420
malus_domestica	MD12G1023300.v1.1 MD14G1020300.v1.1
prunus_persica	Prupe.7G113300_v2.0.a1
pyrus_communis	pycom12g02170 pycom14g01990
rosa_chinensis	RchiOBHm_Chr3g0472921 RchiOBHm_Chr3g0472941
rosa_laevigata	RLG00000024045 RLG00000024046
rosa_multiflora	Rmu_sc0004313.1_g000002 Rmu_sc0021557.1_g000002
rosa_roxburghii	Rroxscaffold_6G00408480 Rroxscaffold_6G00408490
rosa_rugosa	Rorug03G0129600
rosa_samantha	Rh3CG205000 Rh3CG205200
rosa_wichuraiana	Rw3G016170 Rw3G016180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	358
AccB7I	CCANNNNNTGG	1 cut(s)	1300
AccII	CGCG	2 cut(s)	105, 1086
AciI	CCGC	4 cut(s)	105, 557, 577, 986
AclWI	GGATC	4 cut(s)	313, 771, 784, 1034
AcoI	YGGCCR	3 cut(s)	441, 574, 692
AcsI	RAATTY	1 cut(s)	431
AcuI	CTGAAG	1 cut(s)	806
AcyI	GRCGYC	1 cut(s)	857
AfaI	GTAC	5 cut(s)	296, 734, 812, 959, 1136
AfiI	CCNNNNNNNGG	5 cut(s)	104, 151, 613, 1151, 1300
AgsI	TTSAA	4 cut(s)	403, 431, 673, 746
AhlI	ACTAGT	1 cut(s)	128
AjiI	CACGTC	1 cut(s)	763
AjnI	CCWGG	2 cut(s)	686, 1239
AleI	CACNNNNGTG	1 cut(s)	915
AluBI	AGCT	3 cut(s)	309, 1037, 1266
AluI	AGCT	3 cut(s)	309, 1037, 1266
Alw26I	GTCTC	1 cut(s)	53
AlwI	GGATC	4 cut(s)	313, 771, 784, 1034
Ama87I	CYCGRG	1 cut(s)	389
AoxI	GGCC	5 cut(s)	385, 441, 574, 675, 692
ApeKI	GCWGC	1 cut(s)	1263
ApoI	RAATTY	1 cut(s)	431
AspA2I	CCTAGG	1 cut(s)	451
AspS9I	GGNCC	6 cut(s)	142, 338, 386, 717, 756, 1049
AsuC2I	CCSGG	2 cut(s)	525, 754
AsuHPI	GGTGA	6 cut(s)	109, 458, 539, 865, 929, 1082
AsuII	TTCGAA	1 cut(s)	1107
AvaI	CYCGRG	1 cut(s)	389
AvaII	GGWCC	5 cut(s)	142, 338, 717, 756, 1049
AvrII	CCTAGG	1 cut(s)	451
BamHI	GGATCC	1 cut(s)	776
BanII	GRGCYC	1 cut(s)	591
BbvI	GCAGC	1 cut(s)	1275
BccI	CCATC	2 cut(s)	607, 1194
BceAI	ACGGC	1 cut(s)	844
BcgI	CGANNNNNNTGC	2 cut(s)	1169, 1203
BciT130I	CCWGG	2 cut(s)	688, 1241
BcnI	CCSGG	2 cut(s)	525, 754
BcoDI	GTCTC	1 cut(s)	53
BcuI	ACTAGT	1 cut(s)	128
BfaI	CTAG	5 cut(s)	38, 129, 452, 1017, 1094
BfmI	CTRYAG	2 cut(s)	304, 367
BfuAI	ACCTGC	1 cut(s)	358
BisI	GCNGC	2 cut(s)	577, 1264
BlnI	CCTAGG	1 cut(s)	451
BlsI	GCNGC	2 cut(s)	578, 1265
BmcAI	AGTACT	1 cut(s)	296
Bme1390I	CCNGG	4 cut(s)	525, 688, 754, 1241
Bme18I	GGWCC	5 cut(s)	142, 338, 717, 756, 1049
BmeT110I	CYCGRG	1 cut(s)	389
BmgBI	CACGTC	1 cut(s)	763
BmgT120I	GGNCC	6 cut(s)	142, 338, 386, 717, 756, 1049
BmiI	GGNNCC	9 cut(s)	144, 340, 348, 388, 718, 757, 758, 778, 1292
BmrFI	CCNGG	4 cut(s)	525, 688, 754, 1241
BmsI	GCATC	4 cut(s)	654, 687, 784, 1213
BoxI	GACNNNNGTC	2 cut(s)	453, 761
BpmI	CTGGAG	1 cut(s)	294
Bpu14I	TTCGAA	1 cut(s)	1107
BpuMI	CCSGG	2 cut(s)	525, 754
BsaHI	GRCGYC	1 cut(s)	857
BsaJI	CCNNGG	6 cut(s)	342, 451, 523, 608, 860, 887
Bsc4I	CCNNNNNNNGG	5 cut(s)	104, 151, 613, 1151, 1300
Bse118I	RCCGGY	1 cut(s)	443
Bse1I	ACTGG	4 cut(s)	171, 277, 670, 1052
Bse3DI	GCAATG	1 cut(s)	948
BseBI	CCWGG	2 cut(s)	688, 1241
BseDI	CCNNGG	6 cut(s)	342, 451, 523, 608, 860, 887
BseGI	GGATG	6 cut(s)	142, 258, 618, 645, 979, 1205
BseLI	CCNNNNNNNGG	5 cut(s)	104, 151, 613, 1151, 1300
BseMI	GCAATG	1 cut(s)	948
BseMII	CTCAG	1 cut(s)	1180
BseNI	ACTGG	4 cut(s)	171, 277, 670, 1052
BseRI	GAGGAG	3 cut(s)	23, 90, 509
BseX3I	CGGCCG	1 cut(s)	574
BseXI	GCAGC	1 cut(s)	1275
BseYI	CCCAGC	1 cut(s)	772
Bsh1236I	CGCG	2 cut(s)	105, 1086
Bsh1285I	CGRYCG	1 cut(s)	577
BshFI	GGCC	5 cut(s)	387, 443, 576, 677, 694
BsiEI	CGRYCG	1 cut(s)	577
BsiHKCI	CYCGRG	1 cut(s)	389
BsiSI	CCGG	4 cut(s)	444, 525, 753, 1115
BslFI	GGGAC	3 cut(s)	324, 578, 769
BslI	CCNNNNNNNGG	5 cut(s)	104, 151, 613, 1151, 1300
BsmAI	GTCTC	1 cut(s)	53
BsmFI	GGGAC	3 cut(s)	324, 578, 769
BsnI	GGCC	5 cut(s)	387, 443, 576, 677, 694
BsoBI	CYCGRG	1 cut(s)	389
Bsp119I	TTCGAA	1 cut(s)	1107
Bsp1286I	GDGCHC	1 cut(s)	591
Bsp1407I	TGTACA	2 cut(s)	957, 1134
Bsp143I	GATC	5 cut(s)	178, 318, 776, 805, 1039
Bsp68I	TCGCGA	1 cut(s)	1086
BspACI	CCGC	4 cut(s)	105, 557, 577, 986
BspANI	GGCC	5 cut(s)	387, 443, 576, 677, 694
BspCNI	CTCAG	1 cut(s)	1179
BspFNI	CGCG	2 cut(s)	105, 1086
BspLI	GGNNCC	9 cut(s)	144, 340, 348, 388, 718, 757, 758, 778, 1292
BspMI	ACCTGC	1 cut(s)	358
BspPI	GGATC	4 cut(s)	313, 771, 784, 1034
BspT104I	TTCGAA	1 cut(s)	1107
BsrDI	GCAATG	1 cut(s)	948
BsrFI	RCCGGY	1 cut(s)	443
BsrGI	TGTACA	2 cut(s)	957, 1134
BsrI	ACTGG	4 cut(s)	171, 277, 670, 1052
BssAI	RCCGGY	1 cut(s)	443
BssECI	CCNNGG	6 cut(s)	342, 451, 523, 608, 860, 887
BssMI	GATC	5 cut(s)	178, 318, 776, 805, 1039
BssNI	GRCGYC	1 cut(s)	857
BssT1I	CCWWGG	3 cut(s)	342, 451, 608
Bst2UI	CCWGG	2 cut(s)	688, 1241
Bst4CI	ACNGT	2 cut(s)	888, 1076
Bst6I	CTCTTC	2 cut(s)	56, 942
BstACI	GRCGYC	1 cut(s)	857
BstAUI	TGTACA	2 cut(s)	957, 1134
BstBI	TTCGAA	1 cut(s)	1107
BstC8I	GCNNGC	3 cut(s)	212, 598, 882
BstDEI	CTNAG	2 cut(s)	798, 1166
BstDSI	CCRYGG	2 cut(s)	860, 887
BstEII	GGTNACC	1 cut(s)	446
BstF5I	GGATG	6 cut(s)	142, 258, 618, 645, 979, 1205
BstFNI	CGCG	2 cut(s)	105, 1086
BstKTI	GATC	5 cut(s)	181, 321, 779, 808, 1042
BstMAI	GTCTC	1 cut(s)	53
BstMBI	GATC	5 cut(s)	178, 318, 776, 805, 1039
BstMCI	CGRYCG	1 cut(s)	577
BstMWI	GCNNNNNNNGC	2 cut(s)	683, 833
BstNI	CCWGG	2 cut(s)	688, 1241
BstNSI	RCATGY	5 cut(s)	218, 884, 995, 1190, 1278
BstPAI	GACNNNNGTC	2 cut(s)	453, 761
BstPI	GGTNACC	1 cut(s)	446
BstSCI	CCNGG	4 cut(s)	523, 686, 752, 1239
BstSFI	CTRYAG	2 cut(s)	304, 367
BstUI	CGCG	2 cut(s)	105, 1086
BstV1I	GCAGC	1 cut(s)	1275
BstX2I	RGATCY	2 cut(s)	318, 776
BstXI	CCANNNNNNTGG	1 cut(s)	713
BstYI	RGATCY	2 cut(s)	318, 776
BstZI	CGGCCG	1 cut(s)	574
BsuRI	GGCC	5 cut(s)	387, 443, 576, 677, 694
BtgI	CCRYGG	2 cut(s)	860, 887
BtrI	CACGTC	1 cut(s)	763
BtsCI	GGATG	6 cut(s)	142, 258, 618, 645, 979, 1205
BtsI	GCAGTG	1 cut(s)	444
BtsIMutI	CAGTG	3 cut(s)	164, 444, 1059
BtuMI	TCGCGA	1 cut(s)	1086
BveI	ACCTGC	1 cut(s)	358
Cac8I	GCNNGC	3 cut(s)	212, 598, 882
Cfr10I	RCCGGY	1 cut(s)	443
Cfr13I	GGNCC	6 cut(s)	142, 338, 386, 717, 756, 1049
CseI	GACGC	1 cut(s)	865
CsiI	ACCWGGT	1 cut(s)	1239
Csp6I	GTAC	5 cut(s)	295, 733, 811, 958, 1135
CviQI	GTAC	5 cut(s)	295, 733, 811, 958, 1135
DdeI	CTNAG	2 cut(s)	798, 1166
DpnI	GATC	5 cut(s)	180, 320, 778, 807, 1041
DpnII	GATC	5 cut(s)	178, 318, 776, 805, 1039
EaeI	YGGCCR	3 cut(s)	441, 574, 692
EagI	CGGCCG	1 cut(s)	574
Eam1104I	CTCTTC	2 cut(s)	56, 942
EarI	CTCTTC	2 cut(s)	56, 942
EclXI	CGGCCG	1 cut(s)	574
Eco130I	CCWWGG	3 cut(s)	342, 451, 608
Eco147I	AGGCCT	1 cut(s)	677
Eco24I	GRGCYC	1 cut(s)	591
Eco47I	GGWCC	5 cut(s)	142, 338, 717, 756, 1049
Eco52I	CGGCCG	1 cut(s)	574
Eco57I	CTGAAG	1 cut(s)	806
Eco88I	CYCGRG	1 cut(s)	389
Eco91I	GGTNACC	1 cut(s)	446
EcoO109I	RGGNCCY	3 cut(s)	142, 386, 756
EcoO65I	GGTNACC	1 cut(s)	446
EcoRII	CCWGG	2 cut(s)	686, 1239
EcoT14I	CCWWGG	3 cut(s)	342, 451, 608
EcoT22I	ATGCAT	2 cut(s)	1192, 1280
EcoT38I	GRGCYC	1 cut(s)	591
ErhI	CCWWGG	3 cut(s)	342, 451, 608
FaqI	GGGAC	3 cut(s)	324, 578, 769
FauI	CCCGC	1 cut(s)	979
FauNDI	CATATG	1 cut(s)	183
Fnu4HI	GCNGC	2 cut(s)	577, 1264
FokI	GGATG	6 cut(s)	149, 265, 625, 632, 986, 1212
FriOI	GRGCYC	1 cut(s)	591
Fsp4HI	GCNGC	2 cut(s)	577, 1264
FspBI	CTAG	5 cut(s)	38, 129, 452, 1017, 1094
GluI	GCNGC	2 cut(s)	577, 1264
GsaI	CCCAGC	1 cut(s)	776
GsuI	CTGGAG	1 cut(s)	294
HaeIII	GGCC	5 cut(s)	387, 443, 576, 677, 694
HapII	CCGG	4 cut(s)	444, 525, 753, 1115
HgaI	GACGC	1 cut(s)	865
Hin1I	GRCGYC	1 cut(s)	857
HincII	GTYRAC	1 cut(s)	1318
HindII	GTYRAC	1 cut(s)	1318
HinfI	GANTC	3 cut(s)	245, 566, 1217
HpaI	GTTAAC	1 cut(s)	1318
HpaII	CCGG	4 cut(s)	444, 525, 753, 1115
HphI	GGTGA	6 cut(s)	109, 458, 539, 865, 929, 1082
Hpy166II	GTNNAC	3 cut(s)	720, 1024, 1318
Hpy188I	TCNGA	5 cut(s)	136, 459, 494, 805, 1169
Hpy188III	TCNNGA	3 cut(s)	403, 640, 1085
Hpy8I	GTNNAC	3 cut(s)	720, 1024, 1318
HpyAV	CCTTC	9 cut(s)	259, 409, 580, 617, 844, 914, 1082, 1155, 1291
HpyCH4III	ACNGT	2 cut(s)	888, 1076
HpyCH4IV	ACGT	1 cut(s)	762
HpyCH4V	TGCA	7 cut(s)	157, 437, 880, 884, 965, 1190, 1278
HpyF10VI	GCNNNNNNNGC	2 cut(s)	683, 833
HpyF3I	CTNAG	2 cut(s)	798, 1166
HpySE526I	ACGT	1 cut(s)	762
Hsp92I	GRCGYC	1 cut(s)	857
KflI	GGGWCCC	1 cut(s)	756
KspAI	GTTAAC	1 cut(s)	1318
Kzo9I	GATC	5 cut(s)	178, 318, 776, 805, 1039
LmnI	GCTCC	2 cut(s)	1271, 1290
Lsp1109I	GCAGC	1 cut(s)	1275
LweI	GCATC	4 cut(s)	654, 687, 784, 1213
MabI	ACCWGGT	1 cut(s)	1239
MaeI	CTAG	5 cut(s)	38, 129, 452, 1017, 1094
MaeII	ACGT	1 cut(s)	762
MaeIII	GTNAC	7 cut(s)	199, 328, 446, 527, 616, 853, 1070
MalI	GATC	5 cut(s)	180, 320, 778, 807, 1041
MboI	GATC	5 cut(s)	178, 318, 776, 805, 1039
MboII	GAAGA	4 cut(s)	8, 43, 328, 959
MfeI	CAATTG	1 cut(s)	225
MflI	RGATCY	2 cut(s)	318, 776
MhlI	GDGCHC	1 cut(s)	591
MluCI	AATT	5 cut(s)	8, 225, 431, 901, 1325
MlyI	GAGTC	1 cut(s)	560
Mph1103I	ATGCAT	2 cut(s)	1192, 1280
MseI	TTAA	4 cut(s)	219, 1100, 1175, 1317
MslI	CAYNNNNRTG	3 cut(s)	915, 996, 1002
MspI	CCGG	4 cut(s)	444, 525, 753, 1115
MspR9I	CCNGG	4 cut(s)	525, 688, 754, 1241
MunI	CAATTG	1 cut(s)	225
MvaI	CCWGG	2 cut(s)	688, 1241
MvnI	CGCG	2 cut(s)	105, 1086
MwoI	GCNNNNNNNGC	2 cut(s)	683, 833
NciI	CCSGG	2 cut(s)	525, 754
NdeI	CATATG	1 cut(s)	183
NdeII	GATC	5 cut(s)	178, 318, 776, 805, 1039
NlaIV	GGNNCC	9 cut(s)	144, 340, 348, 388, 718, 757, 758, 778, 1292
NmeAIII	GCCGAG	1 cut(s)	1170
NmuCI	GTSAC	5 cut(s)	446, 527, 616, 853, 1070
NruI	TCGCGA	1 cut(s)	1086
NsiI	ATGCAT	2 cut(s)	1192, 1280
NspI	RCATGY	5 cut(s)	218, 884, 995, 1190, 1278
NspV	TTCGAA	1 cut(s)	1107
OliI	CACNNNNGTG	1 cut(s)	915
PaeI	GCATGC	1 cut(s)	884
PceI	AGGCCT	1 cut(s)	677
PfeI	GAWTC	2 cut(s)	245, 1217
PflFI	GACNNNGTC	1 cut(s)	1241
PflMI	CCANNNNNTGG	1 cut(s)	1300
PkrI	GCNGC	2 cut(s)	578, 1265
PleI	GAGTC	1 cut(s)	560
PpsI	GAGTC	1 cut(s)	560
PpuMI	RGGWCCY	2 cut(s)	142, 756
PshAI	GACNNNNGTC	2 cut(s)	453, 761
Psp5II	RGGWCCY	2 cut(s)	142, 756
Psp6I	CCWGG	2 cut(s)	686, 1239
PspEI	GGTNACC	1 cut(s)	446
PspFI	CCCAGC	1 cut(s)	772
PspGI	CCWGG	2 cut(s)	686, 1239
PspN4I	GGNNCC	9 cut(s)	144, 340, 348, 388, 718, 757, 758, 778, 1292
PspPI	GGNCC	6 cut(s)	142, 338, 386, 717, 756, 1049
PspPPI	RGGWCCY	2 cut(s)	142, 756
PsuI	RGATCY	2 cut(s)	318, 776
PsyI	GACNNNGTC	1 cut(s)	1241
RruI	TCGCGA	1 cut(s)	1086
RsaI	GTAC	5 cut(s)	296, 734, 812, 959, 1136
RsaNI	GTAC	5 cut(s)	295, 733, 811, 958, 1135
RseI	CAYNNNNRTG	3 cut(s)	915, 996, 1002
SaqAI	TTAA	4 cut(s)	219, 1100, 1175, 1317
SatI	GCNGC	2 cut(s)	577, 1264
Sau3AI	GATC	5 cut(s)	178, 318, 776, 805, 1039
Sau96I	GGNCC	6 cut(s)	142, 338, 386, 717, 756, 1049
ScaI	AGTACT	1 cut(s)	296
SchI	GAGTC	1 cut(s)	560
ScrFI	CCNGG	4 cut(s)	525, 688, 754, 1241
SduI	GDGCHC	1 cut(s)	591
SexAI	ACCWGGT	1 cut(s)	1239
SfaNI	GCATC	4 cut(s)	654, 687, 784, 1213
SfcI	CTRYAG	2 cut(s)	304, 367
SfuI	TTCGAA	1 cut(s)	1107
SinI	GGWCC	5 cut(s)	142, 338, 717, 756, 1049
SmiMI	CAYNNNNRTG	3 cut(s)	915, 996, 1002
SpeI	ACTAGT	1 cut(s)	128
SphI	GCATGC	1 cut(s)	884
Sse9I	AATT	5 cut(s)	8, 225, 431, 901, 1325
SseBI	AGGCCT	1 cut(s)	677
SsiI	CCGC	4 cut(s)	105, 557, 577, 986
SspI	AATATT	1 cut(s)	114
SspMI	CTAG	5 cut(s)	38, 129, 452, 1017, 1094
StuI	AGGCCT	1 cut(s)	677
StyD4I	CCNGG	4 cut(s)	523, 686, 752, 1239
StyI	CCWWGG	3 cut(s)	342, 451, 608
TaaI	ACNGT	2 cut(s)	888, 1076
TaiI	ACGT	1 cut(s)	765
TaqI	TCGA	5 cut(s)	91, 585, 639, 1107, 1207
TasI	AATT	5 cut(s)	8, 225, 431, 901, 1325
TatI	WGTACW	5 cut(s)	294, 732, 810, 957, 1134
TauI	GCSGC	1 cut(s)	579
TfiI	GAWTC	2 cut(s)	245, 1217
Tru1I	TTAA	4 cut(s)	219, 1100, 1175, 1317
Tru9I	TTAA	4 cut(s)	219, 1100, 1175, 1317
TscAI	CASTG	3 cut(s)	171, 444, 1059
TseFI	GTSAC	5 cut(s)	446, 527, 616, 853, 1070
TseI	GCWGC	1 cut(s)	1263
Tsp45I	GTSAC	5 cut(s)	446, 527, 616, 853, 1070
TspDTI	ATGAA	8 cut(s)	8, 183, 648, 986, 1020, 1047, 1295, 1296
TspGWI	ACGGA	2 cut(s)	1173, 1216
TspRI	CASTG	3 cut(s)	171, 444, 1059
Tth111I	GACNNNGTC	1 cut(s)	1241
Van91I	CCANNNNNTGG	1 cut(s)	1300
VpaK11BI	GGWCC	5 cut(s)	142, 338, 717, 756, 1049
XapI	RAATTY	1 cut(s)	431
XceI	RCATGY	5 cut(s)	218, 884, 995, 1190, 1278
XcmI	CCANNNNNNNNNTGG	2 cut(s)	332, 340
XmaJI	CCTAGG	1 cut(s)	451
XspI	CTAG	5 cut(s)	38, 129, 452, 1017, 1094
ZrmI	AGTACT	1 cut(s)	296
Zsp2I	ATGCAT	2 cut(s)	1192, 1280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.