RchiOBHm_Chr3g0472941

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0010421
Physical Location & Seq
Reverse (-)
18892646 .. 18896455
3810 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43863

Sequence Viewer

Length: 1332 bp
ATGACGATGAAATTGAGCCTGCATCTGTTCTCTTTCCTCCTGACTATCTCTGTTGTCCCTCACCTCCTAAAATCACAAGATGATGATTTTGTTAGGCAACCTGCTGGTAGAGTAATTCTCACTCCTCATGATCGTTCTGACTCTGACCCTCAACAGGTGCATATTTCACTGGTGGGAAAGGACCACATGAAAGTTTCATGGGTTACAGACAACAGTCATACCATATCTATTGTGGAGTATGGAAAGCAATCTGGAAAATATAATGCAAAGTCTACTGGAGAACACACATGGTACGATTATTTTTCATATACTTCTGGGAGGATCCACCATGTTACTATTGGCCCTTTGGAGCCTTCAACCACATACTTCTATAGCTGTGGTGGTTCCGGTCCGGAGTTCTCCTTCAAGACACCCCCACAAACACTTCCGATCGAATTTGTAATAGTTGGTGACCTAGGACAAACTGATTGGACTAACTCCACTCTAGCCCACATCAAAAGCATAGACTACGATGTCCTCCTCTTACCGGGTGATCTATCCTATGCCGATGGACACCAACCCCTTTGGGACTCGTTTGGCCGGATTGTTGAACCCTTGGCGAGCCACCGCCCCTGGATGGTCACAGAAGGCAACCACGAAGTCGAAATCTCTCCCGTCGTTTCCCCAACCGGGTTCAAAGCCTTCAATGCCAGGTGGCAAATGCCGTACAAAGAGAGCGGATCCACCTCGAACTTGTACTACTCTTTTGAAGTTGCTGGCACCCACATCATCATGCTTGGGTCCTACGCTGAGTTTGACACCAAATCGAAGCAGTACGCGTGGCTTGTAGCCGATTTGGCAAAGATTGATAGGAAGAAAACGCCGTGGGTTGTTGTGGTATTGCATGCACCGTGGTATAATACCAACACTGCTCATCAGGGTGAAGGAGAGAGCATGAGAATAGCTATGGAGAAGTTGCTGTATAAGGCACGGGTTGATCTGGTTTATTCAGGGCACGTTCATGCCTATGAGCGATTTACTAGGGTGTACAATAATAAGGCTGACCCATGTGGTCCCGTGCATGTGACCATTGGCGACGGTGGGAACCGAGAAGGATTGGCATTAATGTTCGAAGACCCCCCTTCTCCTCTGTCCTTGTACAGAGAACCGAGTTTTGGACATGGACGACTCAAGATAGTGAATGAAACACACGCCGTTTGGGGATGGCATCGCAACCAGGACTCAAACTTTGTTGTAAAAGACCAAGTGTGGCTACAAAGTTTGAGCTCCTCTAAAATATGCTCCAAATCTGCGGACCTGGAAATTCCACCGTCATCTGAGGATGAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

443

Amino Acids

49.89

Weight (kDa)

6.04

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 50 - 137 1.4e-22 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 146 - 336 6.3e-19 Calcineurin-like phosphoesterase
Metallophos_C PF14008 351 - 408 2.7e-18 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1330
AasI GACNNNNNNGTC 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 109
AccB1I GGYRCC 1 cut(s) 758
AccBSI CCGCTC 1 cut(s) 717
AccI GTMKAC 1 cut(s) 272
AccII CGCG 1 cut(s) 818
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 3 cut(s) 607, 717, 1292
AclWI GGATC 4 cut(s) 316, 329, 714, 727
AcoI YGGCCR 1 cut(s) 577
AcsI RAATTY 2 cut(s) 434, 1302
AfaI GTAC 6 cut(s) 293, 707, 737, 815, 1028, 1139
AfiI CCNNNNNNNGG 5 cut(s) 154, 526, 616, 669, 1154
AflIII ACRYGT 1 cut(s) 816
AgsI TTSAA 6 cut(s) 357, 406, 590, 676, 685, 749
AjnI CCWGG 4 cut(s) 611, 689, 1215, 1296
AjuI GAANNNNNNNTTGG 2 cut(s) 1137, 1169
AluBI AGCT 3 cut(s) 375, 944, 1266
AluI AGCT 3 cut(s) 375, 944, 1266
Alw21I GWGCWC 1 cut(s) 1268
AlwI GGATC 4 cut(s) 316, 329, 714, 727
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 2 cut(s) 340, 577
ApoI RAATTY 2 cut(s) 434, 1302
ArsI GACNNNNNNTTYG 2 cut(s) 1211, 1243
AseI ATTAAT 1 cut(s) 1103
AspA2I CCTAGG 1 cut(s) 454
AspS9I GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1294
AsuC2I CCSGG 2 cut(s) 528, 670
AsuHPI GGTGA 4 cut(s) 53, 461, 542, 932
AsuII TTCGAA 1 cut(s) 1110
AvaII GGWCC 5 cut(s) 181, 389, 780, 1052, 1294
AvrII CCTAGG 1 cut(s) 454
BaeGI GKGCMC 1 cut(s) 996
BamHI GGATCC 2 cut(s) 321, 719
BanI GGYRCC 1 cut(s) 758
BanII GRGCYC 1 cut(s) 1268
BbsI GAAGAC 1 cut(s) 1119
Bbv12I GWGCWC 1 cut(s) 1268
BccI CCATC 3 cut(s) 542, 610, 1197
BceAI ACGGC 3 cut(s) 688, 847, 1178
BciT130I CCWGG 4 cut(s) 613, 691, 1217, 1298
BcnI CCSGG 2 cut(s) 528, 670
BfaI CTAG 3 cut(s) 455, 485, 1020
BfmI CTRYAG 1 cut(s) 370
BfuAI ACCTGC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 836
BlnI CCTAGG 1 cut(s) 454
Bme1390I CCNGG 6 cut(s) 528, 613, 670, 691, 1217, 1298
Bme18I GGWCC 5 cut(s) 181, 389, 780, 1052, 1294
BmgT120I GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1294
BmiI GGNNCC 8 cut(s) 323, 351, 385, 721, 760, 781, 1054, 1085
BmrFI CCNGG 6 cut(s) 528, 613, 670, 691, 1217, 1298
BmsI GCATC 2 cut(s) 31, 1216
BoxI GACNNNNGTC 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 1119
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BpmI CTGGAG 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 1110
BpuEI CTTGAG 1 cut(s) 1154
BpuMI CCSGG 2 cut(s) 528, 670
BsaJI CCNNGG 5 cut(s) 454, 594, 611, 863, 890
BsaWI WCCGGW 2 cut(s) 386, 391
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 526, 616, 669, 1154
Bse1I ACTGG 2 cut(s) 174, 280
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 4 cut(s) 613, 691, 1217, 1298
BseDI CCNNGG 5 cut(s) 454, 594, 611, 863, 890
BseGI GGATG 3 cut(s) 621, 1208, 1327
BseLI CCNNNNNNNGG 5 cut(s) 154, 526, 616, 669, 1154
BseMII CTCAG 2 cut(s) 780, 1308
BseNI ACTGG 2 cut(s) 174, 280
BseRI GAGGAG 4 cut(s) 114, 509, 1116, 1258
BseSI GKGCMC 1 cut(s) 996
Bsh1236I CGCG 1 cut(s) 818
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 2 cut(s) 342, 579
BshNI GGYRCC 1 cut(s) 758
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 1268
BsiSI CCGG 5 cut(s) 387, 392, 527, 580, 669
BslFI GGGAC 3 cut(s) 41, 581, 1038
BslI CCNNNNNNNGG 5 cut(s) 154, 526, 616, 669, 1154
BsmFI GGGAC 3 cut(s) 41, 581, 1038
BsnI GGCC 2 cut(s) 342, 579
Bsp119I TTCGAA 1 cut(s) 1110
Bsp1286I GDGCHC 2 cut(s) 996, 1268
Bsp13I TCCGGA 1 cut(s) 391
Bsp1407I TGTACA 2 cut(s) 1026, 1137
Bsp143I GATC 6 cut(s) 130, 321, 429, 532, 719, 976
BspACI CCGC 3 cut(s) 607, 717, 1292
BspANI GGCC 2 cut(s) 342, 579
BspCNI CTCAG 2 cut(s) 781, 1309
BspEI TCCGGA 1 cut(s) 391
BspFNI CGCG 1 cut(s) 818
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 8 cut(s) 323, 351, 385, 721, 760, 781, 1054, 1085
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 4 cut(s) 316, 329, 714, 727
BspT104I TTCGAA 1 cut(s) 1110
BspT107I GGYRCC 1 cut(s) 758
BsrBI CCGCTC 1 cut(s) 717
BsrGI TGTACA 2 cut(s) 1026, 1137
BsrI ACTGG 2 cut(s) 174, 280
BssECI CCNNGG 5 cut(s) 454, 594, 611, 863, 890
BssMI GATC 6 cut(s) 130, 321, 429, 532, 719, 976
BssT1I CCWWGG 2 cut(s) 454, 594
Bst2UI CCWGG 4 cut(s) 613, 691, 1217, 1298
Bst4CI ACNGT 4 cut(s) 215, 891, 1079, 1311
BstAUI TGTACA 2 cut(s) 1026, 1137
BstBI TTCGAA 1 cut(s) 1110
BstC8I GCNNGC 4 cut(s) 20, 601, 757, 885
BstDEI CTNAG 2 cut(s) 789, 1317
BstDSI CCRYGG 2 cut(s) 863, 890
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 3 cut(s) 621, 1208, 1327
BstFNI CGCG 1 cut(s) 818
BstKTI GATC 6 cut(s) 133, 324, 432, 535, 722, 979
BstMBI GATC 6 cut(s) 130, 321, 429, 532, 719, 976
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 2 cut(s) 686, 836
BstNI CCWGG 4 cut(s) 613, 691, 1217, 1298
BstNSI RCATGY 2 cut(s) 887, 1064
BstPAI GACNNNNGTC 1 cut(s) 213
BstPI GGTNACC 1 cut(s) 449
BstSCI CCNGG 6 cut(s) 526, 611, 668, 689, 1215, 1296
BstSFI CTRYAG 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 996
BstUI CGCG 1 cut(s) 818
BstV2I GAAGAC 1 cut(s) 1119
BstX2I RGATCY 2 cut(s) 321, 719
BstYI RGATCY 2 cut(s) 321, 719
BsuRI GGCC 2 cut(s) 342, 579
BtgI CCRYGG 2 cut(s) 863, 890
BtgZI GCGATG 1 cut(s) 1193
BtsCI GGATG 3 cut(s) 621, 1208, 1327
BtsI GCAGTG 1 cut(s) 906
BtsIMutI CAGTG 2 cut(s) 167, 906
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 4 cut(s) 20, 601, 757, 885
CciI TCATGA 1 cut(s) 127
Cfr13I GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1294
CpoI CGGWCCG 1 cut(s) 389
Csp6I GTAC 6 cut(s) 292, 706, 736, 814, 1027, 1138
CspI CGGWCCG 1 cut(s) 389
CviQI GTAC 6 cut(s) 292, 706, 736, 814, 1027, 1138
DdeI CTNAG 2 cut(s) 789, 1317
DpnI GATC 6 cut(s) 132, 323, 431, 534, 721, 978
DpnII GATC 6 cut(s) 130, 321, 429, 532, 719, 976
DrdI GACNNNNNNGTC 1 cut(s) 512
DseDI GACNNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 577
Ecl136II GAGCTC 1 cut(s) 1266
Eco130I CCWWGG 2 cut(s) 454, 594
Eco24I GRGCYC 1 cut(s) 1268
Eco47I GGWCC 5 cut(s) 181, 389, 780, 1052, 1294
Eco53kI GAGCTC 1 cut(s) 1266
Eco91I GGTNACC 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 1266
EcoO109I RGGNCCY 1 cut(s) 780
EcoO65I GGTNACC 1 cut(s) 449
EcoRII CCWGG 4 cut(s) 611, 689, 1215, 1296
EcoT14I CCWWGG 2 cut(s) 454, 594
EcoT38I GRGCYC 1 cut(s) 1268
ErhI CCWWGG 2 cut(s) 454, 594
FaqI GGGAC 3 cut(s) 41, 581, 1038
FblI GTMKAC 1 cut(s) 272
FokI GGATG 2 cut(s) 628, 1215
FriOI GRGCYC 1 cut(s) 1268
FspBI CTAG 3 cut(s) 455, 485, 1020
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 2 cut(s) 342, 579
HapII CCGG 5 cut(s) 387, 392, 527, 580, 669
HinfI GANTC 4 cut(s) 140, 569, 1167, 1220
HpaII CCGG 5 cut(s) 387, 392, 527, 580, 669
HphI GGTGA 4 cut(s) 53, 461, 542, 932
Hpy166II GTNNAC 2 cut(s) 273, 1027
Hpy188I TCNGA 4 cut(s) 139, 145, 429, 1318
Hpy188III TCNNGA 6 cut(s) 40, 128, 252, 392, 406, 1171
Hpy8I GTNNAC 2 cut(s) 273, 1027
Hpy99I CGWCG 2 cut(s) 659, 1079
HpyAV CCTTC 7 cut(s) 363, 412, 620, 691, 917, 1085, 1131
HpyCH4III ACNGT 4 cut(s) 215, 891, 1079, 1311
HpyCH4IV ACGT 1 cut(s) 996
HpyCH4V TGCA 6 cut(s) 22, 160, 266, 883, 887, 1060
HpyF10VI GCNNNNNNNGC 2 cut(s) 686, 836
HpyF3I CTNAG 2 cut(s) 789, 1317
HpySE526I ACGT 1 cut(s) 996
Kpn2I TCCGGA 1 cut(s) 391
Kzo9I GATC 6 cut(s) 130, 321, 429, 532, 719, 976
LmnI GCTCC 3 cut(s) 349, 1271, 1286
LweI GCATC 2 cut(s) 31, 1216
MaeI CTAG 3 cut(s) 455, 485, 1020
MaeII ACGT 1 cut(s) 996
MaeIII GTNAC 5 cut(s) 202, 331, 449, 619, 1063
MalI GATC 6 cut(s) 132, 323, 431, 534, 721, 978
MbiI CCGCTC 1 cut(s) 717
MboI GATC 6 cut(s) 130, 321, 429, 532, 719, 976
MboII GAAGA 2 cut(s) 865, 1124
MflI RGATCY 2 cut(s) 321, 719
MhlI GDGCHC 2 cut(s) 996, 1268
MluCI AATT 5 cut(s) 11, 114, 434, 1302, 1325
MluI ACGCGT 1 cut(s) 816
MlyI GAGTC 4 cut(s) 134, 563, 1161, 1214
MroI TCCGGA 1 cut(s) 391
MseI TTAA 1 cut(s) 1103
MslI CAYNNNNRTG 4 cut(s) 770, 918, 999, 1005
MspI CCGG 5 cut(s) 387, 392, 527, 580, 669
MspR9I CCNGG 6 cut(s) 528, 613, 670, 691, 1217, 1298
MvaI CCWGG 4 cut(s) 613, 691, 1217, 1298
MvnI CGCG 1 cut(s) 818
MwoI GCNNNNNNNGC 2 cut(s) 686, 836
NciI CCSGG 2 cut(s) 528, 670
NdeII GATC 6 cut(s) 130, 321, 429, 532, 719, 976
NlaIV GGNNCC 8 cut(s) 323, 351, 385, 721, 760, 781, 1054, 1085
NmuCI GTSAC 3 cut(s) 449, 619, 1063
NspI RCATGY 2 cut(s) 887, 1064
NspV TTCGAA 1 cut(s) 1110
PaeI GCATGC 1 cut(s) 887
PagI TCATGA 1 cut(s) 127
Ple19I CGATCG 1 cut(s) 432
PleI GAGTC 4 cut(s) 134, 563, 1161, 1214
PpsI GAGTC 4 cut(s) 134, 563, 1161, 1214
PpuMI RGGWCCY 1 cut(s) 780
PshAI GACNNNNGTC 1 cut(s) 213
PshBI ATTAAT 1 cut(s) 1103
PsiI TTATAA 1 cut(s) 1330
Psp124BI GAGCTC 1 cut(s) 1268
Psp5II RGGWCCY 1 cut(s) 780
Psp6I CCWGG 4 cut(s) 611, 689, 1215, 1296
PspEI GGTNACC 1 cut(s) 449
PspGI CCWGG 4 cut(s) 611, 689, 1215, 1296
PspN4I GGNNCC 8 cut(s) 323, 351, 385, 721, 760, 781, 1054, 1085
PspPI GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1294
PspPPI RGGWCCY 1 cut(s) 780
PsrI GAACNNNNNNTAC 2 cut(s) 722, 754
PsuI RGATCY 2 cut(s) 321, 719
PvuI CGATCG 1 cut(s) 432
RsaI GTAC 6 cut(s) 293, 707, 737, 815, 1028, 1139
RsaNI GTAC 6 cut(s) 292, 706, 736, 814, 1027, 1138
RseI CAYNNNNRTG 4 cut(s) 770, 918, 999, 1005
Rsr2I CGGWCCG 1 cut(s) 389
RsrII CGGWCCG 1 cut(s) 389
SacI GAGCTC 1 cut(s) 1268
SaqAI TTAA 1 cut(s) 1103
Sau3AI GATC 6 cut(s) 130, 321, 429, 532, 719, 976
Sau96I GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1294
SchI GAGTC 4 cut(s) 134, 563, 1161, 1214
ScrFI CCNGG 6 cut(s) 528, 613, 670, 691, 1217, 1298
SduI GDGCHC 2 cut(s) 996, 1268
SfaNI GCATC 2 cut(s) 31, 1216
SfcI CTRYAG 1 cut(s) 370
SfuI TTCGAA 1 cut(s) 1110
SinI GGWCC 5 cut(s) 181, 389, 780, 1052, 1294
SmiMI CAYNNNNRTG 4 cut(s) 770, 918, 999, 1005
SmlI CTYRAG 1 cut(s) 1169
SmoI CTYRAG 1 cut(s) 1169
SphI GCATGC 1 cut(s) 887
Sse9I AATT 5 cut(s) 11, 114, 434, 1302, 1325
SsiI CCGC 3 cut(s) 607, 717, 1292
SspMI CTAG 3 cut(s) 455, 485, 1020
SstI GAGCTC 1 cut(s) 1268
StyD4I CCNGG 6 cut(s) 526, 611, 668, 689, 1215, 1296
StyI CCWWGG 2 cut(s) 454, 594
TaaI ACNGT 4 cut(s) 215, 891, 1079, 1311
TaiI ACGT 1 cut(s) 999
TaqI TCGA 5 cut(s) 432, 642, 728, 806, 1110
TasI AATT 5 cut(s) 11, 114, 434, 1302, 1325
TatI WGTACW 3 cut(s) 735, 1026, 1137
Tru1I TTAA 1 cut(s) 1103
Tru9I TTAA 1 cut(s) 1103
TscAI CASTG 2 cut(s) 174, 913
TseFI GTSAC 3 cut(s) 449, 619, 1063
Tsp45I GTSAC 3 cut(s) 449, 619, 1063
TspDTI ATGAA 6 cut(s) 23, 186, 203, 294, 989, 1197
TspRI CASTG 2 cut(s) 174, 913
VpaK11BI GGWCC 5 cut(s) 181, 389, 780, 1052, 1294
VspI ATTAAT 1 cut(s) 1103
XapI RAATTY 2 cut(s) 434, 1302
XceI RCATGY 2 cut(s) 887, 1064
XcmI CCANNNNNNNNNTGG 2 cut(s) 229, 335
XmaJI CCTAGG 1 cut(s) 454
XmiI GTMKAC 1 cut(s) 272
XspI CTAG 3 cut(s) 455, 485, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.