Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0010421

Physical Location & Seq

Genomic Coordinates Reverse (-)

1870297 .. 1874981

Gene Structure 4685 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g02170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGCCCACCATTTTTTCTTTCTTTCTATTTTTACTTTTTGGTGTGGAACGTCTGTGGCTTTGCTGTAAGTATTGCCAAGACCCCTTCAAACATATATATACTTACTTCACGCCATTGGAATCTTATTATTTTATCCATTTTCCAAACCCCCTCAACCCTCTTATATATCTCTCCAAGGCTCTGAAAATATATGTATCCAATAGCTCTCACAAAACAAAAATGGAGAAATTATATCTTCATGTGTTCTCTCTCCTCCTAGTCATCTGTCTCTTCCCTCAACTCATAAAATCACAAGATGATGATTATGTTAGACAACCTCCTCGCGGAGTAATATTCACCCACCACACTAGATCGGATGAGGACCCTCAACAGGTGCATATTTCACTGGTGGGAAAAGATCATATGAGAGTTTCATGGGTTACTGAAAGCAAGCATGTTAAATCAATTGTGGAGTATGGAAAGGAATCTGGGATATATAATGGGAAGGCAACTGGAGAACACACATCATACAAGTACTTTCTCTACAGCTCCGGGAAGATCCACCATGTTACCATTGGTCCCTTGGAGCCTGCCACTACATACTTCTATAGGTGTGGAGGTTCAGGCCCCGAGTTCTCCTTCAAGACACCCCCACAAAAACTTCCCCTTGAATTTGCAGTGGCCGGTGACCTAGGTCAGACTGAATGGACTAACTCAACCTTACAACACATCGGAAGCATGGACTACGATGTGCTTCTCCTCCCCGGTGACTTGTCATACGCCGATACACAGCAACCGCTTTGGGACTCCTTCGGCCGCATTGTCGAGCCCTACGCCAGCAAACGCCCGTGGATGGTCACAGAAGGCAACCATGAAATCGAGAGCATCCCGATCATCAACCCTACAGGCTTCAAGGCCTACAATGCCAGGTGGCCGATGCCCTACCAAGAGAGTGGGTCCACCTCAAACTTGTACTACTCTTTTGAAGTTGCCGGGACCCACGTCATCATGCTGGGGTCCTACGCTGATTTTGATGTTAAGTCAGATCAGTACAAGTGGCTTCAGGCTGATTTGGCAAAGATTGATAGGAAGGTGACGCCGTGGATTGTTGTGCTTCTGCATGCACCTTGGTATAATTCCAATAATGCCCATAAAGGTGAAGGGGAGAGTATGAGGAAAGCAATGGAAGAGTTGCTGTACAATGCAAGGGTGGATGTGGTTTTTGCAGGGCATGTTCATGCGTATGAACGATTTACTAGGATTTACAACAATGAAGCTGATCCATGTGGTCCAGTGTATATGACCATTGGTGACGGTGGAAACCGCGAAGGACTAGCGTTAAAGTTCGAAAATCCGGCTTCCCCTCTGTCTTTGTACAGAGAGCCAAGTTTTGGACACGGAAGGCTCAGAGTATTAAACGAAACACATGCATTTTGGGGATGGCATCGAAACAACGAATCAAACTCCGTTGTAAAAGACCAGGTCTGGTTAGAAAGTTTGAGCAGCTCCAAAACATGCATGAAATCTGGGAGCCAGAAGGTGGGTTCATCATCTGTTAACGATGAATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

517

Amino Acids

59.0

Weight (kDa)

6.52

Isoelectric Point (pI)

36.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	122 - 209	1.5e-23	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	218 - 408	2.7e-18	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	423 - 486	2.3e-17	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010421)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52820
fragaria_vesca	FvH4_6g18411 FvH4_6g18420
malus_domestica	MD12G1023300.v1.1 MD14G1020300.v1.1
prunus_persica	Prupe.7G113300_v2.0.a1
pyrus_communis	pycom12g02170 pycom14g01990
rosa_chinensis	RchiOBHm_Chr3g0472921 RchiOBHm_Chr3g0472941
rosa_laevigata	RLG00000024045 RLG00000024046
rosa_multiflora	Rmu_sc0004313.1_g000002 Rmu_sc0021557.1_g000002
rosa_roxburghii	Rroxscaffold_6G00408480 Rroxscaffold_6G00408490
rosa_rugosa	Rorug03G0129600
rosa_samantha	Rh3CG205000 Rh3CG205200
rosa_wichuraiana	Rw3G016170 Rw3G016180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	1370, 1519
AccII	CGCG	2 cut(s)	324, 1305
AciI	CCGC	4 cut(s)	324, 776, 796, 1303
AclWI	GGATC	2 cut(s)	532, 1253
AcoI	YGGCCR	3 cut(s)	660, 793, 911
AcsI	RAATTY	1 cut(s)	650
AcuI	CTGAAG	1 cut(s)	1025
AcyI	GRCGYC	1 cut(s)	1076
AfaI	GTAC	5 cut(s)	515, 953, 1031, 1178, 1355
AfiI	CCNNNNNNNGG	5 cut(s)	323, 370, 832, 1370, 1519
AgsI	TTSAA	5 cut(s)	88, 622, 650, 892, 965
AjiI	CACGTC	1 cut(s)	982
AjnI	CCWGG	2 cut(s)	905, 1458
AluBI	AGCT	4 cut(s)	204, 528, 1256, 1485
AluI	AGCT	4 cut(s)	204, 528, 1256, 1485
Alw26I	GTCTC	1 cut(s)	272
AlwI	GGATC	2 cut(s)	532, 1253
Ama87I	CYCGRG	1 cut(s)	608
AoxI	GGCC	5 cut(s)	604, 660, 793, 894, 911
ApeKI	GCWGC	1 cut(s)	1482
ApoI	RAATTY	1 cut(s)	650
AspA2I	CCTAGG	1 cut(s)	670
AspS9I	GGNCC	7 cut(s)	361, 557, 605, 936, 975, 996, 1268
AsuC2I	CCSGG	3 cut(s)	532, 744, 973
AsuHPI	GGTGA	6 cut(s)	328, 677, 758, 1084, 1148, 1301
AsuII	TTCGAA	1 cut(s)	1326
AvaI	CYCGRG	1 cut(s)	608
AvaII	GGWCC	6 cut(s)	361, 557, 936, 975, 996, 1268
AvrII	CCTAGG	1 cut(s)	670
BanII	GRGCYC	1 cut(s)	810
BbvI	GCAGC	1 cut(s)	1494
BccI	CCATC	2 cut(s)	826, 1413
BceAI	ACGGC	1 cut(s)	1063
BcgI	CGANNNNNNTGC	2 cut(s)	1388, 1422
BciT130I	CCWGG	2 cut(s)	907, 1460
BciVI	GTATCC	1 cut(s)	205
BcnI	CCSGG	3 cut(s)	532, 744, 973
BcoDI	GTCTC	1 cut(s)	272
BfaI	CTAG	5 cut(s)	257, 348, 671, 1236, 1313
BfmI	CTRYAG	3 cut(s)	523, 586, 882
BfuI	GTATCC	1 cut(s)	205
BisI	GCNGC	2 cut(s)	796, 1483
BlnI	CCTAGG	1 cut(s)	670
BlsI	GCNGC	2 cut(s)	797, 1484
BmcAI	AGTACT	1 cut(s)	515
Bme1390I	CCNGG	5 cut(s)	532, 744, 907, 973, 1460
Bme18I	GGWCC	6 cut(s)	361, 557, 936, 975, 996, 1268
BmeT110I	CYCGRG	1 cut(s)	608
BmgBI	CACGTC	1 cut(s)	982
BmgT120I	GGNCC	7 cut(s)	361, 557, 605, 936, 975, 996, 1268
BmiI	GGNNCC	9 cut(s)	363, 559, 567, 607, 937, 976, 977, 997, 1511
BmrFI	CCNGG	5 cut(s)	532, 744, 907, 973, 1460
BmsI	GCATC	3 cut(s)	873, 906, 1432
BoxI	GACNNNNGTC	2 cut(s)	672, 980
BpmI	CTGGAG	1 cut(s)	513
Bpu14I	TTCGAA	1 cut(s)	1326
BpuMI	CCSGG	3 cut(s)	532, 744, 973
BsaHI	GRCGYC	1 cut(s)	1076
BsaJI	CCNNGG	7 cut(s)	174, 561, 670, 742, 827, 1079, 1106
Bsc4I	CCNNNNNNNGG	5 cut(s)	323, 370, 832, 1370, 1519
Bse118I	RCCGGY	1 cut(s)	662
Bse1I	ACTGG	3 cut(s)	390, 496, 1271
Bse3DI	GCAATG	1 cut(s)	1167
BseBI	CCWGG	2 cut(s)	907, 1460
BseDI	CCNNGG	7 cut(s)	174, 561, 670, 742, 827, 1079, 1106
BseGI	GGATG	5 cut(s)	361, 837, 864, 1198, 1424
BseLI	CCNNNNNNNGG	5 cut(s)	323, 370, 832, 1370, 1519
BseMI	GCAATG	1 cut(s)	1167
BseMII	CTCAG	1 cut(s)	1399
BseNI	ACTGG	3 cut(s)	390, 496, 1271
BseRI	GAGGAG	3 cut(s)	242, 309, 728
BseX3I	CGGCCG	1 cut(s)	793
BseXI	GCAGC	1 cut(s)	1494
BseYI	CCCAGC	1 cut(s)	991
Bsh1236I	CGCG	2 cut(s)	324, 1305
Bsh1285I	CGRYCG	1 cut(s)	796
BshFI	GGCC	5 cut(s)	606, 662, 795, 896, 913
BsiEI	CGRYCG	1 cut(s)	796
BsiHKCI	CYCGRG	1 cut(s)	608
BsiSI	CCGG	5 cut(s)	531, 663, 744, 972, 1334
BslFI	GGGAC	3 cut(s)	543, 797, 988
BslI	CCNNNNNNNGG	5 cut(s)	323, 370, 832, 1370, 1519
BsmAI	GTCTC	1 cut(s)	272
BsmFI	GGGAC	3 cut(s)	543, 797, 988
BsnI	GGCC	5 cut(s)	606, 662, 795, 896, 913
BsoBI	CYCGRG	1 cut(s)	608
Bsp119I	TTCGAA	1 cut(s)	1326
Bsp1286I	GDGCHC	1 cut(s)	810
Bsp1407I	TGTACA	2 cut(s)	1176, 1353
Bsp143I	GATC	6 cut(s)	350, 397, 537, 870, 1024, 1258
BspACI	CCGC	4 cut(s)	324, 776, 796, 1303
BspANI	GGCC	5 cut(s)	606, 662, 795, 896, 913
BspCNI	CTCAG	1 cut(s)	1398
BspFNI	CGCG	2 cut(s)	324, 1305
BspLI	GGNNCC	9 cut(s)	363, 559, 567, 607, 937, 976, 977, 997, 1511
BspPI	GGATC	2 cut(s)	532, 1253
BspT104I	TTCGAA	1 cut(s)	1326
BsrDI	GCAATG	1 cut(s)	1167
BsrFI	RCCGGY	1 cut(s)	662
BsrGI	TGTACA	2 cut(s)	1176, 1353
BsrI	ACTGG	3 cut(s)	390, 496, 1271
BssAI	RCCGGY	1 cut(s)	662
BssECI	CCNNGG	7 cut(s)	174, 561, 670, 742, 827, 1079, 1106
BssMI	GATC	6 cut(s)	350, 397, 537, 870, 1024, 1258
BssNI	GRCGYC	1 cut(s)	1076
BssT1I	CCWWGG	4 cut(s)	174, 561, 670, 1106
Bst2UI	CCWGG	2 cut(s)	907, 1460
Bst4CI	ACNGT	1 cut(s)	1295
Bst6I	CTCTTC	2 cut(s)	275, 1161
BstACI	GRCGYC	1 cut(s)	1076
BstAUI	TGTACA	2 cut(s)	1176, 1353
BstBI	TTCGAA	1 cut(s)	1326
BstC8I	GCNNGC	4 cut(s)	431, 570, 817, 1101
BstDEI	CTNAG	1 cut(s)	1385
BstDSI	CCRYGG	2 cut(s)	827, 1079
BstEII	GGTNACC	1 cut(s)	665
BstF5I	GGATG	5 cut(s)	361, 837, 864, 1198, 1424
BstFNI	CGCG	2 cut(s)	324, 1305
BstKTI	GATC	6 cut(s)	353, 400, 540, 873, 1027, 1261
BstMAI	GTCTC	1 cut(s)	272
BstMBI	GATC	6 cut(s)	350, 397, 537, 870, 1024, 1258
BstMCI	CGRYCG	1 cut(s)	796
BstMWI	GCNNNNNNNGC	2 cut(s)	902, 1052
BstNI	CCWGG	2 cut(s)	907, 1460
BstNSI	RCATGY	5 cut(s)	437, 1103, 1214, 1409, 1497
BstPAI	GACNNNNGTC	2 cut(s)	672, 980
BstPI	GGTNACC	1 cut(s)	665
BstSCI	CCNGG	5 cut(s)	530, 742, 905, 971, 1458
BstSFI	CTRYAG	3 cut(s)	523, 586, 882
BstUI	CGCG	2 cut(s)	324, 1305
BstV1I	GCAGC	1 cut(s)	1494
BstX2I	RGATCY	1 cut(s)	537
BstXI	CCANNNNNNTGG	1 cut(s)	932
BstYI	RGATCY	1 cut(s)	537
BstZI	CGGCCG	1 cut(s)	793
BsuI	GTATCC	1 cut(s)	205
BsuRI	GGCC	5 cut(s)	606, 662, 795, 896, 913
BtgI	CCRYGG	2 cut(s)	827, 1079
BtrI	CACGTC	1 cut(s)	982
BtsCI	GGATG	5 cut(s)	361, 837, 864, 1198, 1424
BtsI	GCAGTG	1 cut(s)	663
BtsIMutI	CAGTG	3 cut(s)	383, 663, 1278
Cac8I	GCNNGC	4 cut(s)	431, 570, 817, 1101
Cfr10I	RCCGGY	1 cut(s)	662
Cfr13I	GGNCC	7 cut(s)	361, 557, 605, 936, 975, 996, 1268
CseI	GACGC	1 cut(s)	1084
CsiI	ACCWGGT	1 cut(s)	1458
Csp6I	GTAC	5 cut(s)	514, 952, 1030, 1177, 1354
CviQI	GTAC	5 cut(s)	514, 952, 1030, 1177, 1354
DdeI	CTNAG	1 cut(s)	1385
DpnI	GATC	6 cut(s)	352, 399, 539, 872, 1026, 1260
DpnII	GATC	6 cut(s)	350, 397, 537, 870, 1024, 1258
EaeI	YGGCCR	3 cut(s)	660, 793, 911
EagI	CGGCCG	1 cut(s)	793
Eam1104I	CTCTTC	2 cut(s)	275, 1161
EarI	CTCTTC	2 cut(s)	275, 1161
EclXI	CGGCCG	1 cut(s)	793
Eco130I	CCWWGG	4 cut(s)	174, 561, 670, 1106
Eco147I	AGGCCT	1 cut(s)	896
Eco24I	GRGCYC	1 cut(s)	810
Eco47I	GGWCC	6 cut(s)	361, 557, 936, 975, 996, 1268
Eco52I	CGGCCG	1 cut(s)	793
Eco57I	CTGAAG	1 cut(s)	1025
Eco88I	CYCGRG	1 cut(s)	608
Eco91I	GGTNACC	1 cut(s)	665
EcoO109I	RGGNCCY	4 cut(s)	361, 605, 975, 996
EcoO65I	GGTNACC	1 cut(s)	665
EcoRII	CCWGG	2 cut(s)	905, 1458
EcoT14I	CCWWGG	4 cut(s)	174, 561, 670, 1106
EcoT22I	ATGCAT	2 cut(s)	1411, 1499
EcoT38I	GRGCYC	1 cut(s)	810
ErhI	CCWWGG	4 cut(s)	174, 561, 670, 1106
FaqI	GGGAC	3 cut(s)	543, 797, 988
FauNDI	CATATG	1 cut(s)	402
Fnu4HI	GCNGC	2 cut(s)	796, 1483
FokI	GGATG	5 cut(s)	368, 844, 851, 1205, 1431
FriOI	GRGCYC	1 cut(s)	810
Fsp4HI	GCNGC	2 cut(s)	796, 1483
FspBI	CTAG	5 cut(s)	257, 348, 671, 1236, 1313
GluI	GCNGC	2 cut(s)	796, 1483
GsaI	CCCAGC	1 cut(s)	995
GsuI	CTGGAG	1 cut(s)	513
HaeIII	GGCC	5 cut(s)	606, 662, 795, 896, 913
HapII	CCGG	5 cut(s)	531, 663, 744, 972, 1334
HgaI	GACGC	1 cut(s)	1084
Hin1I	GRCGYC	1 cut(s)	1076
HincII	GTYRAC	1 cut(s)	1537
HindII	GTYRAC	1 cut(s)	1537
HinfI	GANTC	4 cut(s)	119, 464, 785, 1436
HpaI	GTTAAC	1 cut(s)	1537
HpaII	CCGG	5 cut(s)	531, 663, 744, 972, 1334
HphI	GGTGA	6 cut(s)	328, 677, 758, 1084, 1148, 1301
Hpy166II	GTNNAC	2 cut(s)	939, 1537
Hpy188I	TCNGA	6 cut(s)	183, 355, 678, 713, 1024, 1388
Hpy188III	TCNNGA	3 cut(s)	622, 859, 868
Hpy8I	GTNNAC	2 cut(s)	939, 1537
HpyCH4III	ACNGT	1 cut(s)	1295
HpyCH4IV	ACGT	2 cut(s)	49, 981
HpyCH4V	TGCA	8 cut(s)	376, 656, 1099, 1103, 1184, 1205, 1409, 1497
HpyF10VI	GCNNNNNNNGC	2 cut(s)	902, 1052
HpyF3I	CTNAG	1 cut(s)	1385
HpySE526I	ACGT	2 cut(s)	49, 981
Hsp92I	GRCGYC	1 cut(s)	1076
KflI	GGGWCCC	1 cut(s)	975
KspAI	GTTAAC	1 cut(s)	1537
Kzo9I	GATC	6 cut(s)	350, 397, 537, 870, 1024, 1258
LmnI	GCTCC	4 cut(s)	533, 565, 1490, 1509
Lsp1109I	GCAGC	1 cut(s)	1494
LweI	GCATC	3 cut(s)	873, 906, 1432
MabI	ACCWGGT	1 cut(s)	1458
MaeI	CTAG	5 cut(s)	257, 348, 671, 1236, 1313
MaeII	ACGT	2 cut(s)	49, 981
MaeIII	GTNAC	7 cut(s)	418, 547, 665, 746, 835, 1072, 1289
MalI	GATC	6 cut(s)	352, 399, 539, 872, 1026, 1260
MboI	GATC	6 cut(s)	350, 397, 537, 870, 1024, 1258
MboII	GAAGA	4 cut(s)	227, 262, 547, 1178
MfeI	CAATTG	1 cut(s)	444
MflI	RGATCY	1 cut(s)	537
MhlI	GDGCHC	1 cut(s)	810
MluCI	AATT	5 cut(s)	227, 444, 650, 1114, 1544
MlyI	GAGTC	1 cut(s)	779
Mph1103I	ATGCAT	2 cut(s)	1411, 1499
MseI	TTAA	5 cut(s)	438, 1017, 1319, 1394, 1536
MslI	CAYNNNNRTG	3 cut(s)	1134, 1215, 1221
MspI	CCGG	5 cut(s)	531, 663, 744, 972, 1334
MspR9I	CCNGG	5 cut(s)	532, 744, 907, 973, 1460
MunI	CAATTG	1 cut(s)	444
MvaI	CCWGG	2 cut(s)	907, 1460
MvnI	CGCG	2 cut(s)	324, 1305
MwoI	GCNNNNNNNGC	2 cut(s)	902, 1052
NciI	CCSGG	3 cut(s)	532, 744, 973
NdeI	CATATG	1 cut(s)	402
NdeII	GATC	6 cut(s)	350, 397, 537, 870, 1024, 1258
NlaIV	GGNNCC	9 cut(s)	363, 559, 567, 607, 937, 976, 977, 997, 1511
NmuCI	GTSAC	5 cut(s)	665, 746, 835, 1072, 1289
NsiI	ATGCAT	2 cut(s)	1411, 1499
NspI	RCATGY	5 cut(s)	437, 1103, 1214, 1409, 1497
NspV	TTCGAA	1 cut(s)	1326
PaeI	GCATGC	1 cut(s)	1103
PceI	AGGCCT	1 cut(s)	896
PfeI	GAWTC	3 cut(s)	119, 464, 1436
PflFI	GACNNNGTC	1 cut(s)	1460
PflMI	CCANNNNNTGG	2 cut(s)	1370, 1519
PfoI	TCCNGGA	1 cut(s)	530
PkrI	GCNGC	2 cut(s)	797, 1484
PleI	GAGTC	1 cut(s)	779
PpsI	GAGTC	1 cut(s)	779
PpuMI	RGGWCCY	3 cut(s)	361, 975, 996
PshAI	GACNNNNGTC	2 cut(s)	672, 980
Psp5II	RGGWCCY	3 cut(s)	361, 975, 996
Psp6I	CCWGG	2 cut(s)	905, 1458
PspEI	GGTNACC	1 cut(s)	665
PspFI	CCCAGC	1 cut(s)	991
PspGI	CCWGG	2 cut(s)	905, 1458
PspN4I	GGNNCC	9 cut(s)	363, 559, 567, 607, 937, 976, 977, 997, 1511
PspPI	GGNCC	7 cut(s)	361, 557, 605, 936, 975, 996, 1268
PspPPI	RGGWCCY	3 cut(s)	361, 975, 996
PsuI	RGATCY	1 cut(s)	537
PsyI	GACNNNGTC	1 cut(s)	1460
RsaI	GTAC	5 cut(s)	515, 953, 1031, 1178, 1355
RsaNI	GTAC	5 cut(s)	514, 952, 1030, 1177, 1354
RseI	CAYNNNNRTG	3 cut(s)	1134, 1215, 1221
SaqAI	TTAA	5 cut(s)	438, 1017, 1319, 1394, 1536
SatI	GCNGC	2 cut(s)	796, 1483
Sau3AI	GATC	6 cut(s)	350, 397, 537, 870, 1024, 1258
Sau96I	GGNCC	7 cut(s)	361, 557, 605, 936, 975, 996, 1268
ScaI	AGTACT	1 cut(s)	515
SchI	GAGTC	1 cut(s)	779
ScrFI	CCNGG	5 cut(s)	532, 744, 907, 973, 1460
SduI	GDGCHC	1 cut(s)	810
SexAI	ACCWGGT	1 cut(s)	1458
SfaNI	GCATC	3 cut(s)	873, 906, 1432
SfcI	CTRYAG	3 cut(s)	523, 586, 882
SfuI	TTCGAA	1 cut(s)	1326
SinI	GGWCC	6 cut(s)	361, 557, 936, 975, 996, 1268
SmiMI	CAYNNNNRTG	3 cut(s)	1134, 1215, 1221
SphI	GCATGC	1 cut(s)	1103
Sse9I	AATT	5 cut(s)	227, 444, 650, 1114, 1544
SseBI	AGGCCT	1 cut(s)	896
SsiI	CCGC	4 cut(s)	324, 776, 796, 1303
SspI	AATATT	1 cut(s)	333
SspMI	CTAG	5 cut(s)	257, 348, 671, 1236, 1313
StuI	AGGCCT	1 cut(s)	896
StyD4I	CCNGG	5 cut(s)	530, 742, 905, 971, 1458
StyI	CCWWGG	4 cut(s)	174, 561, 670, 1106
TaaI	ACNGT	1 cut(s)	1295
TaiI	ACGT	2 cut(s)	52, 984
TaqI	TCGA	4 cut(s)	804, 858, 1326, 1426
TasI	AATT	5 cut(s)	227, 444, 650, 1114, 1544
TatI	WGTACW	5 cut(s)	513, 951, 1029, 1176, 1353
TauI	GCSGC	1 cut(s)	798
TfiI	GAWTC	3 cut(s)	119, 464, 1436
Tru1I	TTAA	5 cut(s)	438, 1017, 1319, 1394, 1536
Tru9I	TTAA	5 cut(s)	438, 1017, 1319, 1394, 1536
TscAI	CASTG	3 cut(s)	390, 663, 1278
TseFI	GTSAC	5 cut(s)	665, 746, 835, 1072, 1289
TseI	GCWGC	1 cut(s)	1482
Tsp45I	GTSAC	5 cut(s)	665, 746, 835, 1072, 1289
TspDTI	ATGAA	8 cut(s)	227, 402, 867, 1205, 1239, 1266, 1514, 1515
TspGWI	ACGGA	2 cut(s)	1392, 1435
TspRI	CASTG	3 cut(s)	390, 663, 1278
Tth111I	GACNNNGTC	1 cut(s)	1460
Van91I	CCANNNNNTGG	2 cut(s)	1370, 1519
VpaK11BI	GGWCC	6 cut(s)	361, 557, 936, 975, 996, 1268
XapI	RAATTY	1 cut(s)	650
XceI	RCATGY	5 cut(s)	437, 1103, 1214, 1409, 1497
XcmI	CCANNNNNNNNNTGG	2 cut(s)	551, 559
XmaJI	CCTAGG	1 cut(s)	670
XspI	CTAG	5 cut(s)	257, 348, 671, 1236, 1313
ZrmI	AGTACT	1 cut(s)	515
Zsp2I	ATGCAT	2 cut(s)	1411, 1499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.