Rw3G016170

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010421
Physical Location & Seq
Forward (+)
16913337 .. 16916720
3384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016170.1

Sequence Viewer

Length: 1296 bp
ATGACGATGAAATTGAGCCTGCATATGTTCTCTTTCCTCCTGACTATCTCTGTTGTCCCTTACCTGCTAAAATCACAAGATGATGATTTTGTTAGGCAACCTGCTGGTAGAGTAATTCTCACTCCTCATGATCGTTCTGACTCTGACCCTCAACAGGTGCATATTTCACTGGTGGGAAAGGACCACATGAAAGTTTCATGGGTTACAGACAACAGTCATACCAAATCTATTGTGGAGTATGGAAAGCAATCTGGAAAATATAATGCAAAGTCTACTGGAGAACACACATGGTACGATTATTTTTCATATACTTCTGGGAGGATCCACCATGTTACTATTGGCCCCTTGGAGCCTTCAACCACATACTTCTATAGCTGTGGTGGTTCCGGTCCGGAGTTCTCCTTCAAGACACCCCCACAAACACTTCCGATCGAATTTGTAATAGTTGGTGACCTAGGACAAACTGATTGGACTAACTCCACTCTAGCCCACATCAAAAGCATAGACTACGATGTCCTCCTCTTACCGGGTGATCTATCCTATGCCGATGGACACCAACCCCTTTGGGACTCGTTTGGCCGGATTGTTGAACCCTTGGCGAGCCACCGCCCCTGGATGGTCACAGAAGGCAACCACGAAGTCGAAATCGCTCCCGTCGTTTCCCCAACCGGGTTCAAAGCCTTCAATGCCAGGTGGCAAATGCCGTACAAAGAGAGCGGATCCACCTCGAACTTGTACTACTCTTTTGAAGTTGCTGGAACCCACATCATCATGCTTGGGTCCTACGCTGAGTTTGACACCAAATCGAAGCAATACGCGTGGCTTGTAGCCGATTTGGCAAAGATTGATAGGAAGAAAACGCCGTGGGTTGTTGTGGTATTGCATGCACCGTGGTATAATACCAACACTGCTCATCAGGGTGAAGGAGAGAGCATGAGAATAGCTATGGAGAAGTTGCTGTATAAGGCACGGGTTGATCTGGTTTATTCAGGGCACGTTCATGCCTATGAGCGATTTACTAGGGTGTACAATAATAAGGCTGACCCATGTGGTCCCGTGCATGTGACCATTGGCGACGACCCCCCTTCTCCTCTGTCCTTGTACAGAGAACCGAGTTTTGGACATGGACGACTCAAGATAGTGAATGGAACACACGCCGTTTGGGGATGGCATCGCAACCAGGACTCAAACTTTGTTGTAAAAGACCAAGTCTGGCTACAAAGTTTGAGCTCCTCTAAAATATGCTCCAAATCTGCGGACCTGGAAGTTCCACCGTCATCTAAGGATGAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

431

Amino Acids

48.57

Weight (kDa)

6.39

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 50 - 137 3.1e-23 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 146 - 336 5e-19 Calcineurin-like phosphoesterase
Metallophos_C PF14008 352 - 396 1.7e-08 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1294
AasI GACNNNNNNGTC 1 cut(s) 512
Acc36I ACCTGC 2 cut(s) 72, 109
AccBSI CCGCTC 1 cut(s) 717
AccI GTMKAC 1 cut(s) 272
AccII CGCG 1 cut(s) 818
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 3 cut(s) 607, 717, 1256
AclWI GGATC 4 cut(s) 316, 329, 714, 727
AcoI YGGCCR 1 cut(s) 577
AcsI RAATTY 1 cut(s) 434
AfaI GTAC 5 cut(s) 293, 707, 737, 1028, 1103
AfiI CCNNNNNNNGG 5 cut(s) 154, 526, 616, 669, 1118
AflIII ACRYGT 1 cut(s) 816
AgsI TTSAA 6 cut(s) 357, 406, 590, 676, 685, 749
AjnI CCWGG 4 cut(s) 611, 689, 1179, 1260
AjuI GAANNNNNNNTTGG 2 cut(s) 1101, 1133
AluBI AGCT 3 cut(s) 375, 944, 1230
AluI AGCT 3 cut(s) 375, 944, 1230
Alw21I GWGCWC 1 cut(s) 1232
AlwI GGATC 4 cut(s) 316, 329, 714, 727
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 2 cut(s) 340, 577
ApoI RAATTY 1 cut(s) 434
ArsI GACNNNNNNTTYG 2 cut(s) 1175, 1207
AspA2I CCTAGG 1 cut(s) 454
AspS9I GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1258
AsuC2I CCSGG 2 cut(s) 528, 670
AsuHPI GGTGA 3 cut(s) 461, 542, 932
AvaII GGWCC 5 cut(s) 181, 389, 780, 1052, 1258
AvrII CCTAGG 1 cut(s) 454
BaeGI GKGCMC 1 cut(s) 996
BamHI GGATCC 2 cut(s) 321, 719
BanII GRGCYC 1 cut(s) 1232
Bbv12I GWGCWC 1 cut(s) 1232
BccI CCATC 3 cut(s) 542, 610, 1161
BceAI ACGGC 3 cut(s) 688, 847, 1142
BciT130I CCWGG 4 cut(s) 613, 691, 1181, 1262
BcnI CCSGG 2 cut(s) 528, 670
BfaI CTAG 3 cut(s) 455, 485, 1020
BfmI CTRYAG 1 cut(s) 370
BfuAI ACCTGC 2 cut(s) 72, 109
BglI GCCNNNNNGGC 1 cut(s) 836
BlnI CCTAGG 1 cut(s) 454
Bme1390I CCNGG 6 cut(s) 528, 613, 670, 691, 1181, 1262
Bme18I GGWCC 5 cut(s) 181, 389, 780, 1052, 1258
BmgT120I GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1258
BmiI GGNNCC 8 cut(s) 323, 343, 351, 385, 721, 760, 781, 1054
BmrFI CCNGG 6 cut(s) 528, 613, 670, 691, 1181, 1262
BmsI GCATC 1 cut(s) 1180
BoxI GACNNNNGTC 1 cut(s) 213
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BpmI CTGGAG 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 1118
BpuMI CCSGG 2 cut(s) 528, 670
BsaJI CCNNGG 6 cut(s) 345, 454, 594, 611, 863, 890
BsaWI WCCGGW 2 cut(s) 386, 391
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 526, 616, 669, 1118
Bse1I ACTGG 2 cut(s) 174, 280
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 4 cut(s) 613, 691, 1181, 1262
BseDI CCNNGG 6 cut(s) 345, 454, 594, 611, 863, 890
BseGI GGATG 3 cut(s) 621, 1172, 1291
BseLI CCNNNNNNNGG 5 cut(s) 154, 526, 616, 669, 1118
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 2 cut(s) 174, 280
BseRI GAGGAG 4 cut(s) 114, 509, 1080, 1222
BseSI GKGCMC 1 cut(s) 996
Bsh1236I CGCG 1 cut(s) 818
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 2 cut(s) 342, 579
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 1232
BsiSI CCGG 5 cut(s) 387, 392, 527, 580, 669
BslFI GGGAC 3 cut(s) 41, 581, 1038
BslI CCNNNNNNNGG 5 cut(s) 154, 526, 616, 669, 1118
BsmFI GGGAC 3 cut(s) 41, 581, 1038
BsnI GGCC 2 cut(s) 342, 579
Bsp1286I GDGCHC 2 cut(s) 996, 1232
Bsp13I TCCGGA 1 cut(s) 391
Bsp1407I TGTACA 2 cut(s) 1026, 1101
Bsp143I GATC 6 cut(s) 130, 321, 429, 532, 719, 976
BspACI CCGC 3 cut(s) 607, 717, 1256
BspANI GGCC 2 cut(s) 342, 579
BspCNI CTCAG 1 cut(s) 781
BspEI TCCGGA 1 cut(s) 391
BspFNI CGCG 1 cut(s) 818
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 8 cut(s) 323, 343, 351, 385, 721, 760, 781, 1054
BspMI ACCTGC 2 cut(s) 72, 109
BspPI GGATC 4 cut(s) 316, 329, 714, 727
BsrBI CCGCTC 1 cut(s) 717
BsrGI TGTACA 2 cut(s) 1026, 1101
BsrI ACTGG 2 cut(s) 174, 280
BssECI CCNNGG 6 cut(s) 345, 454, 594, 611, 863, 890
BssMI GATC 6 cut(s) 130, 321, 429, 532, 719, 976
BssT1I CCWWGG 3 cut(s) 345, 454, 594
Bst2UI CCWGG 4 cut(s) 613, 691, 1181, 1262
Bst4CI ACNGT 3 cut(s) 215, 891, 1275
BstAUI TGTACA 2 cut(s) 1026, 1101
BstC8I GCNNGC 3 cut(s) 20, 601, 885
BstDEI CTNAG 2 cut(s) 789, 1281
BstDSI CCRYGG 2 cut(s) 863, 890
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 3 cut(s) 621, 1172, 1291
BstFNI CGCG 1 cut(s) 818
BstKTI GATC 6 cut(s) 133, 324, 432, 535, 722, 979
BstMBI GATC 6 cut(s) 130, 321, 429, 532, 719, 976
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 2 cut(s) 686, 836
BstNI CCWGG 4 cut(s) 613, 691, 1181, 1262
BstNSI RCATGY 2 cut(s) 887, 1064
BstPAI GACNNNNGTC 1 cut(s) 213
BstPI GGTNACC 1 cut(s) 449
BstSCI CCNGG 6 cut(s) 526, 611, 668, 689, 1179, 1260
BstSFI CTRYAG 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 996
BstUI CGCG 1 cut(s) 818
BstX2I RGATCY 2 cut(s) 321, 719
BstYI RGATCY 2 cut(s) 321, 719
BsuRI GGCC 2 cut(s) 342, 579
BtgI CCRYGG 2 cut(s) 863, 890
BtgZI GCGATG 1 cut(s) 1157
BtsCI GGATG 3 cut(s) 621, 1172, 1291
BtsI GCAGTG 1 cut(s) 906
BtsIMutI CAGTG 2 cut(s) 167, 906
BveI ACCTGC 2 cut(s) 72, 109
Cac8I GCNNGC 3 cut(s) 20, 601, 885
CciI TCATGA 1 cut(s) 127
Cfr13I GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1258
CpoI CGGWCCG 1 cut(s) 389
Csp6I GTAC 5 cut(s) 292, 706, 736, 1027, 1102
CspCI CAANNNNNGTGG 2 cut(s) 800, 835
CspI CGGWCCG 1 cut(s) 389
CviQI GTAC 5 cut(s) 292, 706, 736, 1027, 1102
DdeI CTNAG 2 cut(s) 789, 1281
DpnI GATC 6 cut(s) 132, 323, 431, 534, 721, 978
DpnII GATC 6 cut(s) 130, 321, 429, 532, 719, 976
DrdI GACNNNNNNGTC 1 cut(s) 512
DseDI GACNNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 577
Ecl136II GAGCTC 1 cut(s) 1230
Eco130I CCWWGG 3 cut(s) 345, 454, 594
Eco24I GRGCYC 1 cut(s) 1232
Eco47I GGWCC 5 cut(s) 181, 389, 780, 1052, 1258
Eco53kI GAGCTC 1 cut(s) 1230
Eco91I GGTNACC 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 1230
EcoO109I RGGNCCY 1 cut(s) 780
EcoO65I GGTNACC 1 cut(s) 449
EcoRII CCWGG 4 cut(s) 611, 689, 1179, 1260
EcoT14I CCWWGG 3 cut(s) 345, 454, 594
EcoT38I GRGCYC 1 cut(s) 1232
ErhI CCWWGG 3 cut(s) 345, 454, 594
FaqI GGGAC 3 cut(s) 41, 581, 1038
FauNDI CATATG 1 cut(s) 24
FblI GTMKAC 1 cut(s) 272
FokI GGATG 2 cut(s) 628, 1179
FriOI GRGCYC 1 cut(s) 1232
FspBI CTAG 3 cut(s) 455, 485, 1020
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 2 cut(s) 342, 579
HapII CCGG 5 cut(s) 387, 392, 527, 580, 669
HinfI GANTC 4 cut(s) 140, 569, 1131, 1184
HpaII CCGG 5 cut(s) 387, 392, 527, 580, 669
HphI GGTGA 3 cut(s) 461, 542, 932
Hpy166II GTNNAC 2 cut(s) 273, 1027
Hpy188I TCNGA 3 cut(s) 139, 145, 429
Hpy188III TCNNGA 6 cut(s) 40, 128, 252, 392, 406, 1135
Hpy8I GTNNAC 2 cut(s) 273, 1027
Hpy99I CGWCG 2 cut(s) 659, 1079
HpyAV CCTTC 6 cut(s) 363, 412, 620, 691, 917, 1095
HpyCH4III ACNGT 3 cut(s) 215, 891, 1275
HpyCH4IV ACGT 1 cut(s) 996
HpyCH4V TGCA 6 cut(s) 22, 160, 266, 883, 887, 1060
HpyF10VI GCNNNNNNNGC 2 cut(s) 686, 836
HpyF3I CTNAG 2 cut(s) 789, 1281
HpySE526I ACGT 1 cut(s) 996
Kpn2I TCCGGA 1 cut(s) 391
Kzo9I GATC 6 cut(s) 130, 321, 429, 532, 719, 976
LmnI GCTCC 4 cut(s) 349, 655, 1235, 1250
LweI GCATC 1 cut(s) 1180
MaeI CTAG 3 cut(s) 455, 485, 1020
MaeII ACGT 1 cut(s) 996
MaeIII GTNAC 5 cut(s) 202, 331, 449, 619, 1063
MalI GATC 6 cut(s) 132, 323, 431, 534, 721, 978
MbiI CCGCTC 1 cut(s) 717
MboI GATC 6 cut(s) 130, 321, 429, 532, 719, 976
MboII GAAGA 1 cut(s) 865
MflI RGATCY 2 cut(s) 321, 719
MhlI GDGCHC 2 cut(s) 996, 1232
MluCI AATT 4 cut(s) 11, 114, 434, 1289
MluI ACGCGT 1 cut(s) 816
MlyI GAGTC 4 cut(s) 134, 563, 1125, 1178
MnlI CCTC 9 cut(s) 47, 135, 159, 312, 527, 530, 736, 1101, 1243
MroI TCCGGA 1 cut(s) 391
MslI CAYNNNNRTG 4 cut(s) 770, 918, 999, 1005
MspI CCGG 5 cut(s) 387, 392, 527, 580, 669
MspR9I CCNGG 6 cut(s) 528, 613, 670, 691, 1181, 1262
MvaI CCWGG 4 cut(s) 613, 691, 1181, 1262
MvnI CGCG 1 cut(s) 818
MwoI GCNNNNNNNGC 2 cut(s) 686, 836
NciI CCSGG 2 cut(s) 528, 670
NdeI CATATG 1 cut(s) 24
NdeII GATC 6 cut(s) 130, 321, 429, 532, 719, 976
NlaIV GGNNCC 8 cut(s) 323, 343, 351, 385, 721, 760, 781, 1054
NmuCI GTSAC 3 cut(s) 449, 619, 1063
NspI RCATGY 2 cut(s) 887, 1064
PaeI GCATGC 1 cut(s) 887
PagI TCATGA 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 654
PflFI GACNNNGTC 1 cut(s) 1208
Ple19I CGATCG 1 cut(s) 432
PleI GAGTC 4 cut(s) 134, 563, 1125, 1178
PpsI GAGTC 4 cut(s) 134, 563, 1125, 1178
PpuMI RGGWCCY 1 cut(s) 780
PshAI GACNNNNGTC 1 cut(s) 213
PsiI TTATAA 1 cut(s) 1294
Psp124BI GAGCTC 1 cut(s) 1232
Psp5II RGGWCCY 1 cut(s) 780
Psp6I CCWGG 4 cut(s) 611, 689, 1179, 1260
PspEI GGTNACC 1 cut(s) 449
PspGI CCWGG 4 cut(s) 611, 689, 1179, 1260
PspN4I GGNNCC 8 cut(s) 323, 343, 351, 385, 721, 760, 781, 1054
PspPI GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1258
PspPPI RGGWCCY 1 cut(s) 780
PsrI GAACNNNNNNTAC 2 cut(s) 722, 754
PsuI RGATCY 2 cut(s) 321, 719
PsyI GACNNNGTC 1 cut(s) 1208
PvuI CGATCG 1 cut(s) 432
RsaI GTAC 5 cut(s) 293, 707, 737, 1028, 1103
RsaNI GTAC 5 cut(s) 292, 706, 736, 1027, 1102
RseI CAYNNNNRTG 4 cut(s) 770, 918, 999, 1005
Rsr2I CGGWCCG 1 cut(s) 389
RsrII CGGWCCG 1 cut(s) 389
SacI GAGCTC 1 cut(s) 1232
Sau3AI GATC 6 cut(s) 130, 321, 429, 532, 719, 976
Sau96I GGNCC 6 cut(s) 181, 341, 389, 780, 1052, 1258
SchI GAGTC 4 cut(s) 134, 563, 1125, 1178
ScrFI CCNGG 6 cut(s) 528, 613, 670, 691, 1181, 1262
SduI GDGCHC 2 cut(s) 996, 1232
SfaNI GCATC 1 cut(s) 1180
SfcI CTRYAG 1 cut(s) 370
SinI GGWCC 5 cut(s) 181, 389, 780, 1052, 1258
SmiMI CAYNNNNRTG 4 cut(s) 770, 918, 999, 1005
SmlI CTYRAG 1 cut(s) 1133
SmoI CTYRAG 1 cut(s) 1133
SphI GCATGC 1 cut(s) 887
Sse9I AATT 4 cut(s) 11, 114, 434, 1289
SsiI CCGC 3 cut(s) 607, 717, 1256
SspMI CTAG 3 cut(s) 455, 485, 1020
SstI GAGCTC 1 cut(s) 1232
StyD4I CCNGG 6 cut(s) 526, 611, 668, 689, 1179, 1260
StyI CCWWGG 3 cut(s) 345, 454, 594
TaaI ACNGT 3 cut(s) 215, 891, 1275
TaiI ACGT 1 cut(s) 999
TaqI TCGA 4 cut(s) 432, 642, 728, 806
TasI AATT 4 cut(s) 11, 114, 434, 1289
TatI WGTACW 3 cut(s) 735, 1026, 1101
TscAI CASTG 2 cut(s) 174, 913
TseFI GTSAC 3 cut(s) 449, 619, 1063
Tsp45I GTSAC 3 cut(s) 449, 619, 1063
TspDTI ATGAA 5 cut(s) 23, 186, 203, 294, 989
TspRI CASTG 2 cut(s) 174, 913
Tth111I GACNNNGTC 1 cut(s) 1208
VpaK11BI GGWCC 5 cut(s) 181, 389, 780, 1052, 1258
XapI RAATTY 1 cut(s) 434
XceI RCATGY 2 cut(s) 887, 1064
XcmI CCANNNNNNNNNTGG 2 cut(s) 229, 335
XmaJI CCTAGG 1 cut(s) 454
XmiI GTMKAC 1 cut(s) 272
XspI CTAG 3 cut(s) 455, 485, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.