Rroxscaffold_6G00408490

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010421
Physical Location & Seq
Forward (+)
30958267 .. 30962017
3751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408490.1

Sequence Viewer

Length: 1332 bp
ATGAGGAAATTGTGTCTATGTCTTTTCTCTCTACTCCTAACCATCTCTCTCTTCCCTCTACACCTAAAAGCAAAAGATGATGATTTTGTTAGGCAACCTCCACGGAGAGTAATATTCACCCACCATGACCGTTCCGACTCAGACCCTCAACAGGTGCATATTTCACTAGTGGGGAAAGATCACATGAAAGTTTCATGGGTTACAGACAGCAAACATTGCAGTTCTCTTGTAGAGTACGGAAAAGAATCTGGAAATTACAATGCAAAAGCTATTGGAGAACACACATCCTACAAATATTTCTTCTATACCTCTGGGAAGATCCACCATGTTACCATTGGCCCTTTGGAGCCATCAACTACATACTTCTATAGATGTGGAGGTTCTGGCCCTGAGTTCTCCTTCAAAACACCCCCTTCAACACTTCCCCTTGATTTTGTAGTCGTTGGTGACCTAGGACAGACTGAATGGACTAACTCCACCCTCGACCACATCCAAAGTATAGATTATGACGTCCTCATTCTACCCGGCGATCTATCTTATGCCGATACCCACCAACCCCTTTGGGACTCATTCGGCCGCATTGTCGAACCCTATGCCAGCCGCCGGCCATGGATGGTCACAGAAGGCAACCACGAAGTCGAAAGCTTCCCCATCATCTACCCAACAGGGTTCAAAGCCTACAATGCAAGGTGGCCGATGCCCTACCAAGAGAGCGGATCCACCTCAAACTTGTACTACTCTTTCGAAGTTGCTGGCACCCACGTCATCATGCTTGGGTCCTACATGGAGTTTGATGCTGATTCAGACCAGTACAAGTGGCTTCAAGCTGACTTGGCAAAGATTGACCGGAAAAAGACGCCTTGGGTTGTTGCCCTTTTGCATGCACCGTGGTATAATACTAACTCTGCTCATCAAGGTGAAGGAGAGAGCATGAGATTAGCTATGGAAGAGCTGTTGTATAATGCAAAAGTTGATTTGGTTTTTCAAGGCCATGTTCATGCGTATGAACGATTTACCAGGGTTTATAAGAATAAGGCTGACCCATGTGGTCCAATTTACATAACCATTGGCGATGGTGGGAACCGAGAAGGACTAGCATTATCGTTCGAAGACCCTGCTTCCCCTCTCTCCTTGTACAGAGAACCGAGTTTCGGACATGGACGGCTCAAAATGGTGAATGAAACACATGCGTTTTGGGGATGGCATCGCAACAACGATTCAAACTTCGTTGTTAAAGACCAGGTCTGGTTACAAAGTTTGAGCTCCTCTAAAACTTGCTCCAAATCTGTCGACCAGAAGATTACTTCACCGTCCACTAAAGATGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

443

Amino Acids

50.44

Weight (kDa)

6.12

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 49 - 136 4.5e-23 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 145 - 335 1.4e-19 Calcineurin-like phosphoesterase
Metallophos_C PF14008 350 - 407 2.8e-17 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1026
AatII GACGTC 1 cut(s) 513
AccB1I GGYRCC 1 cut(s) 755
AccBSI CCGCTC 1 cut(s) 714
AccI GTMKAC 1 cut(s) 1290
AciI CCGC 3 cut(s) 577, 601, 714
AclWI GGATC 3 cut(s) 313, 711, 724
AcoI YGGCCR 3 cut(s) 574, 605, 692
AcyI GRCGYC 2 cut(s) 510, 857
AfaI GTAC 4 cut(s) 236, 734, 812, 1136
AfiI CCNNNNNNNGG 3 cut(s) 151, 603, 1151
AgsI TTSAA 6 cut(s) 403, 417, 673, 824, 986, 1221
AhlI ACTAGT 1 cut(s) 166
AjiI CACGTC 1 cut(s) 763
AjnI CCWGG 2 cut(s) 1016, 1239
AluBI AGCT 6 cut(s) 269, 645, 827, 941, 952, 1263
AluI AGCT 6 cut(s) 269, 645, 827, 941, 952, 1263
Alw21I GWGCWC 1 cut(s) 1265
AlwI GGATC 3 cut(s) 313, 711, 724
AoxI GGCC 6 cut(s) 337, 385, 574, 605, 692, 988
AspA2I CCTAGG 1 cut(s) 451
AspS9I GGNCC 4 cut(s) 338, 386, 777, 1049
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 5 cut(s) 109, 458, 929, 1186, 1299
AsuII TTCGAA 2 cut(s) 744, 1107
AvaII GGWCC 2 cut(s) 777, 1049
AvrII CCTAGG 1 cut(s) 451
BamHI GGATCC 1 cut(s) 716
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 1 cut(s) 1265
BbsI GAAGAC 1 cut(s) 1116
Bbv12I GWGCWC 1 cut(s) 1265
BccI CCATC 6 cut(s) 50, 358, 607, 659, 1067, 1194
BceAI ACGGC 1 cut(s) 1178
BcgI CGANNNNNNTGC 4 cut(s) 575, 609, 1097, 1131
BciT130I CCWGG 2 cut(s) 1018, 1241
BcnI CCSGG 1 cut(s) 525
BcuI ACTAGT 1 cut(s) 166
BfaI CTAG 3 cut(s) 167, 452, 1094
BfmI CTRYAG 1 cut(s) 367
BisI GCNGC 2 cut(s) 577, 601
BlnI CCTAGG 1 cut(s) 451
BlsI GCNGC 2 cut(s) 578, 602
Bme1390I CCNGG 3 cut(s) 525, 1018, 1241
Bme18I GGWCC 2 cut(s) 777, 1049
BmgBI CACGTC 1 cut(s) 763
BmgT120I GGNCC 4 cut(s) 338, 386, 777, 1049
BmiI GGNNCC 5 cut(s) 348, 718, 757, 778, 1082
BmrFI CCNGG 3 cut(s) 525, 1018, 1241
BmsI GCATC 3 cut(s) 687, 784, 1213
BpiI GAAGAC 1 cut(s) 1116
Bpu14I TTCGAA 2 cut(s) 744, 1107
BpuMI CCSGG 1 cut(s) 525
BsaHI GRCGYC 2 cut(s) 510, 857
BsaJI CCNNGG 6 cut(s) 101, 451, 608, 860, 887, 1017
BsaWI WCCGGW 1 cut(s) 846
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 603, 1151
Bse118I RCCGGY 1 cut(s) 603
Bse1I ACTGG 1 cut(s) 808
Bse3DI GCAATG 1 cut(s) 214
BseBI CCWGG 2 cut(s) 1018, 1241
BseDI CCNNGG 6 cut(s) 101, 451, 608, 860, 887, 1017
BseGI GGATG 4 cut(s) 284, 489, 618, 1205
BseLI CCNNNNNNNGG 3 cut(s) 151, 603, 1151
BseMI GCAATG 1 cut(s) 214
BseMII CTCAG 2 cut(s) 153, 381
BseNI ACTGG 1 cut(s) 808
BseRI GAGGAG 1 cut(s) 1255
BseX3I CGGCCG 1 cut(s) 574
Bsh1285I CGRYCG 1 cut(s) 577
BshFI GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
BshNI GGYRCC 1 cut(s) 755
BsiEI CGRYCG 1 cut(s) 577
BsiHKAI GWGCWC 1 cut(s) 1265
BsiSI CCGG 3 cut(s) 525, 604, 847
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 3 cut(s) 151, 603, 1151
BsmFI GGGAC 1 cut(s) 578
BsnI GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
Bsp119I TTCGAA 2 cut(s) 744, 1107
Bsp1286I GDGCHC 1 cut(s) 1265
Bsp1407I TGTACA 1 cut(s) 1134
Bsp143I GATC 4 cut(s) 178, 318, 529, 716
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 3 cut(s) 577, 601, 714
BspANI GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
BspCNI CTCAG 2 cut(s) 152, 382
BspLI GGNNCC 5 cut(s) 348, 718, 757, 778, 1082
BspPI GGATC 3 cut(s) 313, 711, 724
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 2 cut(s) 744, 1107
BspT107I GGYRCC 1 cut(s) 755
BsrBI CCGCTC 1 cut(s) 714
BsrDI GCAATG 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 603
BsrGI TGTACA 1 cut(s) 1134
BsrI ACTGG 1 cut(s) 808
BssAI RCCGGY 1 cut(s) 603
BssECI CCNNGG 6 cut(s) 101, 451, 608, 860, 887, 1017
BssMI GATC 4 cut(s) 178, 318, 529, 716
BssNI GRCGYC 2 cut(s) 510, 857
BssT1I CCWWGG 3 cut(s) 451, 608, 860
Bst2UI CCWGG 2 cut(s) 1018, 1241
Bst4CI ACNGT 3 cut(s) 131, 888, 1311
Bst6I CTCTTC 2 cut(s) 56, 942
BstACI GRCGYC 2 cut(s) 510, 857
BstAPI GCANNNNNTGC 1 cut(s) 216
BstAUI TGTACA 1 cut(s) 1134
BstBI TTCGAA 2 cut(s) 744, 1107
BstC8I GCNNGC 4 cut(s) 598, 605, 754, 882
BstDEI CTNAG 2 cut(s) 139, 390
BstDSI CCRYGG 3 cut(s) 101, 608, 887
BstEII GGTNACC 1 cut(s) 446
BstF5I GGATG 4 cut(s) 284, 489, 618, 1205
BstKTI GATC 4 cut(s) 181, 321, 532, 719
BstMBI GATC 4 cut(s) 178, 318, 529, 716
BstMCI CGRYCG 1 cut(s) 577
BstMWI GCNNNNNNNGC 3 cut(s) 216, 683, 833
BstNI CCWGG 2 cut(s) 1018, 1241
BstNSI RCATGY 2 cut(s) 884, 1190
BstPI GGTNACC 1 cut(s) 446
BstSCI CCNGG 3 cut(s) 523, 1016, 1239
BstSFI CTRYAG 1 cut(s) 367
BstV2I GAAGAC 1 cut(s) 1116
BstX2I RGATCY 2 cut(s) 318, 716
BstYI RGATCY 2 cut(s) 318, 716
BstZI CGGCCG 1 cut(s) 574
BsuRI GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
BtgI CCRYGG 3 cut(s) 101, 608, 887
BtgZI GCGATG 2 cut(s) 1086, 1190
BtrI CACGTC 1 cut(s) 763
BtsCI GGATG 4 cut(s) 284, 489, 618, 1205
Cac8I GCNNGC 4 cut(s) 598, 605, 754, 882
Cfr10I RCCGGY 1 cut(s) 603
Cfr13I GGNCC 4 cut(s) 338, 386, 777, 1049
CseI GACGC 1 cut(s) 865
CsiI ACCWGGT 1 cut(s) 1239
Csp6I GTAC 4 cut(s) 235, 733, 811, 1135
CviQI GTAC 4 cut(s) 235, 733, 811, 1135
DdeI CTNAG 2 cut(s) 139, 390
DpnI GATC 4 cut(s) 180, 320, 531, 718
DpnII GATC 4 cut(s) 178, 318, 529, 716
EaeI YGGCCR 3 cut(s) 574, 605, 692
EagI CGGCCG 1 cut(s) 574
Eam1104I CTCTTC 2 cut(s) 56, 942
EarI CTCTTC 2 cut(s) 56, 942
Ecl136II GAGCTC 1 cut(s) 1263
EclXI CGGCCG 1 cut(s) 574
Eco130I CCWWGG 3 cut(s) 451, 608, 860
Eco24I GRGCYC 1 cut(s) 1265
Eco47I GGWCC 2 cut(s) 777, 1049
Eco52I CGGCCG 1 cut(s) 574
Eco53kI GAGCTC 1 cut(s) 1263
Eco91I GGTNACC 1 cut(s) 446
EcoICRI GAGCTC 1 cut(s) 1263
EcoO109I RGGNCCY 1 cut(s) 777
EcoO65I GGTNACC 1 cut(s) 446
EcoRII CCWGG 2 cut(s) 1016, 1239
EcoT14I CCWWGG 3 cut(s) 451, 608, 860
EcoT38I GRGCYC 1 cut(s) 1265
ErhI CCWWGG 3 cut(s) 451, 608, 860
FaqI GGGAC 1 cut(s) 578
FblI GTMKAC 1 cut(s) 1290
Fnu4HI GCNGC 2 cut(s) 577, 601
FokI GGATG 4 cut(s) 271, 476, 625, 1212
FriOI GRGCYC 1 cut(s) 1265
Fsp4HI GCNGC 2 cut(s) 577, 601
FspBI CTAG 3 cut(s) 167, 452, 1094
GluI GCNGC 2 cut(s) 577, 601
HaeIII GGCC 6 cut(s) 339, 387, 576, 607, 694, 990
HapII CCGG 3 cut(s) 525, 604, 847
HgaI GACGC 1 cut(s) 865
Hin1I GRCGYC 2 cut(s) 510, 857
HincII GTYRAC 1 cut(s) 1291
HindII GTYRAC 1 cut(s) 1291
HindIII AAGCTT 1 cut(s) 643
HinfI GANTC 5 cut(s) 137, 245, 566, 800, 1217
HpaII CCGG 3 cut(s) 525, 604, 847
HphI GGTGA 5 cut(s) 109, 458, 929, 1186, 1299
Hpy166II GTNNAC 2 cut(s) 1291, 1314
Hpy188I TCNGA 4 cut(s) 136, 142, 805, 1154
Hpy188III TCNNGA 1 cut(s) 249
Hpy8I GTNNAC 2 cut(s) 1291, 1314
HpyAV CCTTC 5 cut(s) 409, 423, 617, 914, 1082
HpyCH4III ACNGT 3 cut(s) 131, 888, 1311
HpyCH4IV ACGT 2 cut(s) 510, 762
HpyCH4V TGCA 7 cut(s) 157, 219, 263, 686, 880, 884, 965
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 683, 833
HpyF3I CTNAG 2 cut(s) 139, 390
HpySE526I ACGT 2 cut(s) 510, 762
Hsp92I GRCGYC 2 cut(s) 510, 857
KroI GCCGGC 1 cut(s) 603
KroNI GCCGGC 1 cut(s) 605
Kzo9I GATC 4 cut(s) 178, 318, 529, 716
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 3 cut(s) 346, 1268, 1283
LweI GCATC 3 cut(s) 687, 784, 1213
MabI ACCWGGT 1 cut(s) 1239
MaeI CTAG 3 cut(s) 167, 452, 1094
MaeII ACGT 2 cut(s) 510, 762
MaeIII GTNAC 5 cut(s) 199, 328, 446, 616, 1248
MalI GATC 4 cut(s) 180, 320, 531, 718
MbiI CCGCTC 1 cut(s) 714
MboI GATC 4 cut(s) 178, 318, 529, 716
MboII GAAGA 6 cut(s) 43, 292, 328, 959, 1121, 1309
MflI RGATCY 2 cut(s) 318, 716
MhlI GDGCHC 1 cut(s) 1265
MluCI AATT 4 cut(s) 8, 253, 1053, 1325
MlyI GAGTC 2 cut(s) 131, 560
MmeI TCCRAC 1 cut(s) 159
MroNI GCCGGC 1 cut(s) 603
MseI TTAA 1 cut(s) 1233
MslI CAYNNNNRTG 3 cut(s) 915, 996, 1002
MspI CCGG 3 cut(s) 525, 604, 847
MspR9I CCNGG 3 cut(s) 525, 1018, 1241
MvaI CCWGG 2 cut(s) 1018, 1241
MwoI GCNNNNNNNGC 3 cut(s) 216, 683, 833
NaeI GCCGGC 1 cut(s) 605
NciI CCSGG 1 cut(s) 525
NcoI CCATGG 1 cut(s) 608
NdeII GATC 4 cut(s) 178, 318, 529, 716
NgoMIV GCCGGC 1 cut(s) 603
NlaIV GGNNCC 5 cut(s) 348, 718, 757, 778, 1082
NmuCI GTSAC 2 cut(s) 446, 616
NspI RCATGY 2 cut(s) 884, 1190
NspV TTCGAA 2 cut(s) 744, 1107
PaeI GCATGC 1 cut(s) 884
PciSI GCTCTTC 1 cut(s) 942
PdiI GCCGGC 1 cut(s) 605
PfeI GAWTC 3 cut(s) 245, 800, 1217
PflFI GACNNNGTC 1 cut(s) 1241
PkrI GCNGC 2 cut(s) 578, 602
PleI GAGTC 2 cut(s) 131, 560
PpsI GAGTC 2 cut(s) 131, 560
PpuMI RGGWCCY 1 cut(s) 777
PsiI TTATAA 1 cut(s) 1026
Psp124BI GAGCTC 1 cut(s) 1265
Psp5II RGGWCCY 1 cut(s) 777
Psp6I CCWGG 2 cut(s) 1016, 1239
PspEI GGTNACC 1 cut(s) 446
PspGI CCWGG 2 cut(s) 1016, 1239
PspN4I GGNNCC 5 cut(s) 348, 718, 757, 778, 1082
PspPI GGNCC 4 cut(s) 338, 386, 777, 1049
PspPPI RGGWCCY 1 cut(s) 777
PsuI RGATCY 2 cut(s) 318, 716
PsyI GACNNNGTC 1 cut(s) 1241
RsaI GTAC 4 cut(s) 236, 734, 812, 1136
RsaNI GTAC 4 cut(s) 235, 733, 811, 1135
RseI CAYNNNNRTG 3 cut(s) 915, 996, 1002
SacI GAGCTC 1 cut(s) 1265
SalI GTCGAC 1 cut(s) 1289
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 1 cut(s) 1233
SatI GCNGC 2 cut(s) 577, 601
Sau3AI GATC 4 cut(s) 178, 318, 529, 716
Sau96I GGNCC 4 cut(s) 338, 386, 777, 1049
SchI GAGTC 2 cut(s) 131, 560
ScrFI CCNGG 3 cut(s) 525, 1018, 1241
SduI GDGCHC 1 cut(s) 1265
SexAI ACCWGGT 1 cut(s) 1239
SfaNI GCATC 3 cut(s) 687, 784, 1213
SfcI CTRYAG 1 cut(s) 367
SfuI TTCGAA 2 cut(s) 744, 1107
SinI GGWCC 2 cut(s) 777, 1049
SmiMI CAYNNNNRTG 3 cut(s) 915, 996, 1002
SpeI ACTAGT 1 cut(s) 166
SphI GCATGC 1 cut(s) 884
Sse9I AATT 4 cut(s) 8, 253, 1053, 1325
SsiI CCGC 3 cut(s) 577, 601, 714
SspI AATATT 2 cut(s) 114, 296
SspMI CTAG 3 cut(s) 167, 452, 1094
SstI GAGCTC 1 cut(s) 1265
StyD4I CCNGG 3 cut(s) 523, 1016, 1239
StyI CCWWGG 3 cut(s) 451, 608, 860
TaaI ACNGT 3 cut(s) 131, 888, 1311
TaiI ACGT 2 cut(s) 513, 765
TaqI TCGA 6 cut(s) 483, 585, 639, 744, 1107, 1290
TasI AATT 4 cut(s) 8, 253, 1053, 1325
TatI WGTACW 3 cut(s) 732, 810, 1134
TauI GCSGC 2 cut(s) 579, 603
TfiI GAWTC 3 cut(s) 245, 800, 1217
Tru1I TTAA 1 cut(s) 1233
Tru9I TTAA 1 cut(s) 1233
TseFI GTSAC 2 cut(s) 446, 616
Tsp45I GTSAC 2 cut(s) 446, 616
TspDTI ATGAA 5 cut(s) 183, 200, 986, 1020, 1194
TspGWI ACGGA 2 cut(s) 118, 252
Tth111I GACNNNGTC 1 cut(s) 1241
VpaK11BI GGWCC 2 cut(s) 777, 1049
XceI RCATGY 2 cut(s) 884, 1190
XcmI CCANNNNNNNNNTGG 2 cut(s) 332, 340
XmaJI CCTAGG 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 1290
XspI CTAG 3 cut(s) 167, 452, 1094
ZraI GACGTC 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.