FvH4_6g19450

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012638
Physical Location & Seq
Reverse (-)
12979511 .. 12982077
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19450.t1

Sequence Viewer

Length: 1008 bp
ATGCAAAGTCCAGCAGAGAAAAAACCAGGCGTCTACACCTACGTGAGTCAATGGCCAATCTACTCCCTGGCCTGGTCAGCTCGCCATGACAAGCCTTCACGCCTCGCCATTGGGAGCTTCATCGAAGACTACAGCAACAAGGTCGAACTCGTCCAGTTCAACTCTTCCACCTCCGACTTCACCACCGACAACCGCCTCATTTTCGACCACCCTTATGCCCCCACGAATCTGATGTTCTTCCCCTCCGATGCGGCCACCAATCCCGACCTCATAGCCACCTCCGGTGACTACTTACGCATTTGGGAGATCCATGACGACCGAATCGAGCTCAAGTCGCTGCTCAATGGCAACATGAGCAGCGAGTTCAACTCGGCCATAACATCATTTGATTGGGCCGAATTTGACACTCGGAGAGTGGCCACATCGAGTGTCGACACAACGTGTACGATTTGGGACATTGAGAGGGAGGCCGTGGACACCCAACTGGTGGCTCATGACAAAGAAGTTTATGACATTTCTTGGGGTGGATTCAACGTGTTCGCTTCTGCTTCTGGGGATGGCACTGTTAGGGTGTTCGACTTGAGAGACAAAGAGAGGTCGACAATAGTATATGAAAACCCTGCTCAAGATGGGTCATTGTTGAGGCTAGAATGGAACAAACATGACCCAAGGTTCATAGCCACGGTTGGAGTGGACAGCAACAAGGTGGTGATATTGGACATTAGGTTTCCCACCGCACCAATGATGGAGCTTAAAAAGCATGGAGCCAGTGTGAATGCCGTTTCTTGGTCGCCACGCATGGGGCACCAGCTATGTTCTGTGAGTGATGATTCAAGGGCTATGATATGGGAGGTGGTGAGAACCGGGTTTGGATCCGATGGGGCGGACGTCGAGCCGGAGATGTGGTACGGAGCGACGGCTCAGATCAATCAAGTGCGGTGGTCACCTGTGGAGTTGGATTGGATTGCCATTGCTTTCATGAACAAGTTGCAGCTATTGAAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

336

Amino Acids

37.87

Weight (kDa)

4.83

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 33 - 283 1e-10 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 100 - 287 1.7e-20 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 104 - 204 1.2e-09 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 117 - 287 1.7e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 123 - 202 4.2e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 125 - 265 1e-15 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 125 - 199 8.5e-10 WDR5 beta-propeller domain
WD40_Gbeta PF25391 134 - 285 1.1e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 137 - 199 5.4e-06 WDR55
Beta-prop_THOC3 PF25174 215 - 285 1e-06 THOC3 beta-propeller domain
Beta-prop_THOC3 PF25174 254 - 323 7.1e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 891
AccB1I GGYRCC 1 cut(s) 804
AccB7I CCANNNNNTGG 1 cut(s) 487
AccI GTMKAC 3 cut(s) 33, 432, 599
AciI CCGC 5 cut(s) 193, 251, 735, 884, 937
AclWI GGATC 3 cut(s) 301, 867, 880
AcoI YGGCCR 4 cut(s) 53, 252, 372, 417
AcsI RAATTY 1 cut(s) 398
AcyI GRCGYC 2 cut(s) 30, 888
AdeI CACNNNGTG 1 cut(s) 441
AfaI GTAC 2 cut(s) 445, 908
AfiI CCNNNNNNNGG 4 cut(s) 72, 487, 786, 800
AflIII ACRYGT 2 cut(s) 440, 534
AgsI TTSAA 5 cut(s) 160, 367, 532, 834, 1000
AjnI CCWGG 3 cut(s) 25, 66, 71
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 6 cut(s) 80, 117, 328, 751, 811, 994
AluI AGCT 6 cut(s) 80, 117, 328, 751, 811, 994
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 579
AlwI GGATC 3 cut(s) 301, 867, 880
AoxI GGCC 7 cut(s) 53, 69, 252, 372, 393, 417, 468
ApeKI GCWGC 3 cut(s) 337, 357, 991
ApoI RAATTY 1 cut(s) 398
AspS9I GGNCC 1 cut(s) 393
AsuC2I CCSGG 1 cut(s) 865
AsuHPI GGTGA 5 cut(s) 172, 296, 721, 868, 936
BaeGI GKGCMC 1 cut(s) 807
BalI TGGCCA 2 cut(s) 55, 419
BamHI GGATCC 1 cut(s) 872
BanI GGYRCC 1 cut(s) 804
BanII GRGCYC 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 3 cut(s) 324, 369, 1003
BccI CCATC 4 cut(s) 551, 623, 739, 872
BceAI ACGGC 3 cut(s) 455, 764, 933
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 3 cut(s) 27, 68, 73
BcnI CCSGG 1 cut(s) 865
BcoDI GTCTC 1 cut(s) 579
BfaI CTAG 1 cut(s) 647
BfmI CTRYAG 1 cut(s) 130
BisI GCNGC 4 cut(s) 252, 338, 358, 992
BlsI GCNGC 4 cut(s) 253, 339, 359, 993
Bme1390I CCNGG 4 cut(s) 27, 68, 73, 865
BmgT120I GGNCC 1 cut(s) 393
BmiI GGNNCC 3 cut(s) 766, 806, 874
BmrFI CCNGG 4 cut(s) 27, 68, 73, 865
BmsI GCATC 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 132
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpuEI CTTGAG 3 cut(s) 314, 601, 609
BpuMI CCSGG 1 cut(s) 865
BsaAI YACGTR 1 cut(s) 43
BsaHI GRCGYC 2 cut(s) 30, 888
BsaJI CCNNGG 4 cut(s) 66, 471, 668, 681
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 4 cut(s) 756, 786, 890, 920
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 487, 786, 800
Bse1I ACTGG 3 cut(s) 154, 489, 768
Bse3DI GCAATG 1 cut(s) 969
BseBI CCWGG 3 cut(s) 27, 68, 73
BseDI CCNNGG 4 cut(s) 66, 471, 668, 681
BseGI GGATG 1 cut(s) 562
BseLI CCNNNNNNNGG 4 cut(s) 72, 487, 786, 800
BseMI GCAATG 1 cut(s) 969
BseMII CTCAG 1 cut(s) 935
BseNI ACTGG 3 cut(s) 154, 489, 768
BseSI GKGCMC 1 cut(s) 807
BseXI GCAGC 3 cut(s) 324, 369, 1003
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 7 cut(s) 55, 71, 254, 374, 395, 419, 470
BshNI GGYRCC 1 cut(s) 804
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 3 cut(s) 282, 864, 896
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 4 cut(s) 72, 487, 786, 800
BsmAI GTCTC 1 cut(s) 579
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 7 cut(s) 55, 71, 254, 374, 395, 419, 470
Bsp1286I GDGCHC 2 cut(s) 330, 807
Bsp143I GATC 3 cut(s) 306, 872, 924
BspACI CCGC 5 cut(s) 193, 251, 735, 884, 937
BspANI GGCC 7 cut(s) 55, 71, 254, 374, 395, 419, 470
BspCNI CTCAG 1 cut(s) 934
BspHI TCATGA 2 cut(s) 493, 978
BspLI GGNNCC 3 cut(s) 766, 806, 874
BspPI GGATC 3 cut(s) 301, 867, 880
BspT107I GGYRCC 1 cut(s) 804
BsrDI GCAATG 1 cut(s) 969
BsrI ACTGG 3 cut(s) 154, 489, 768
BssECI CCNNGG 4 cut(s) 66, 471, 668, 681
BssMI GATC 3 cut(s) 306, 872, 924
BssNI GRCGYC 2 cut(s) 30, 888
BssT1I CCWWGG 1 cut(s) 668
Bst2UI CCWGG 3 cut(s) 27, 68, 73
Bst4CI ACNGT 2 cut(s) 565, 685
Bst6I CTCTTC 1 cut(s) 169
BstACI GRCGYC 2 cut(s) 30, 888
BstBAI YACGTR 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 1 cut(s) 921
BstDSI CCRYGG 2 cut(s) 471, 681
BstEII GGTNACC 1 cut(s) 942
BstF5I GGATG 1 cut(s) 562
BstKTI GATC 3 cut(s) 309, 875, 927
BstMAI GTCTC 1 cut(s) 579
BstMBI GATC 3 cut(s) 306, 872, 924
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 4 cut(s) 77, 334, 354, 757
BstNI CCWGG 3 cut(s) 27, 68, 73
BstPI GGTNACC 1 cut(s) 942
BstSCI CCNGG 4 cut(s) 25, 66, 71, 863
BstSFI CTRYAG 1 cut(s) 130
BstSLI GKGCMC 1 cut(s) 807
BstV1I GCAGC 3 cut(s) 324, 369, 1003
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 2 cut(s) 306, 872
BstYI RGATCY 2 cut(s) 306, 872
BsuRI GGCC 7 cut(s) 55, 71, 254, 374, 395, 419, 470
BtgI CCRYGG 2 cut(s) 471, 681
BtsCI GGATG 1 cut(s) 562
BtsIMutI CAGTG 2 cut(s) 561, 775
Cac8I GCNNGC 1 cut(s) 82
CciI TCATGA 2 cut(s) 493, 978
Cfr13I GGNCC 1 cut(s) 393
CseI GACGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 444, 907
CspCI CAANNNNNGTGG 2 cut(s) 920, 955
CviAII CATG 8 cut(s) 86, 311, 352, 494, 662, 761, 799, 979
CviQI GTAC 2 cut(s) 444, 907
DdeI CTNAG 1 cut(s) 921
DpnI GATC 3 cut(s) 308, 874, 926
DpnII GATC 3 cut(s) 306, 872, 924
DraIII CACNNNGTG 1 cut(s) 441
EaeI YGGCCR 4 cut(s) 53, 252, 372, 417
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
EciI GGCGGA 1 cut(s) 899
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 668
Eco24I GRGCYC 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 328
Eco91I GGTNACC 1 cut(s) 942
EcoICRI GAGCTC 1 cut(s) 328
EcoO65I GGTNACC 1 cut(s) 942
EcoRII CCWGG 3 cut(s) 25, 66, 71
EcoT14I CCWWGG 1 cut(s) 668
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 668
FaeI CATG 8 cut(s) 89, 314, 355, 497, 665, 764, 802, 982
FaqI GGGAC 1 cut(s) 467
FatI CATG 8 cut(s) 85, 310, 351, 493, 661, 760, 798, 978
FblI GTMKAC 3 cut(s) 33, 432, 599
Fnu4HI GCNGC 4 cut(s) 252, 338, 358, 992
FokI GGATG 1 cut(s) 569
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 4 cut(s) 252, 338, 358, 992
FspBI CTAG 1 cut(s) 647
GluI GCNGC 4 cut(s) 252, 338, 358, 992
HaeIII GGCC 7 cut(s) 55, 71, 254, 374, 395, 419, 470
HapII CCGG 3 cut(s) 282, 864, 896
HgaI GACGC 1 cut(s) 19
Hin1I GRCGYC 2 cut(s) 30, 888
Hin1II CATG 8 cut(s) 89, 314, 355, 497, 665, 764, 802, 982
HincII GTYRAC 2 cut(s) 433, 600
HindII GTYRAC 2 cut(s) 433, 600
HinfI GANTC 5 cut(s) 46, 226, 321, 528, 830
HpaII CCGG 3 cut(s) 282, 864, 896
HphI GGTGA 5 cut(s) 172, 296, 721, 868, 936
Hpy166II GTNNAC 6 cut(s) 34, 433, 444, 475, 600, 694
Hpy188I TCNGA 6 cut(s) 175, 231, 247, 411, 877, 924
Hpy188III TCNNGA 4 cut(s) 263, 494, 626, 979
Hpy8I GTNNAC 6 cut(s) 34, 433, 444, 475, 600, 694
Hpy99I CGWCG 2 cut(s) 893, 919
HpyAV CCTTC 2 cut(s) 105, 994
HpyCH4III ACNGT 2 cut(s) 565, 685
HpyCH4IV ACGT 4 cut(s) 42, 440, 534, 888
HpyCH4V TGCA 2 cut(s) 4, 991
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 334, 354, 757
HpyF3I CTNAG 1 cut(s) 921
HpySE526I ACGT 4 cut(s) 42, 440, 534, 888
Hsp92I GRCGYC 2 cut(s) 30, 888
Hsp92II CATG 8 cut(s) 89, 314, 355, 497, 665, 764, 802, 982
Kzo9I GATC 3 cut(s) 306, 872, 924
LmnI GCTCC 4 cut(s) 114, 748, 764, 911
Lsp1109I GCAGC 3 cut(s) 324, 369, 1003
LweI GCATC 1 cut(s) 238
MaeI CTAG 1 cut(s) 647
MaeII ACGT 4 cut(s) 42, 440, 534, 888
MaeIII GTNAC 2 cut(s) 284, 942
MalI GATC 3 cut(s) 308, 874, 926
MboI GATC 3 cut(s) 306, 872, 924
MboII GAAGA 3 cut(s) 137, 156, 229
MflI RGATCY 2 cut(s) 306, 872
MhlI GDGCHC 2 cut(s) 330, 807
MlsI TGGCCA 2 cut(s) 55, 419
MluCI AATT 1 cut(s) 398
MluNI TGGCCA 2 cut(s) 55, 419
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 3 cut(s) 198, 667, 936
Mox20I TGGCCA 2 cut(s) 55, 419
MscI TGGCCA 2 cut(s) 55, 419
MseI TTAA 1 cut(s) 753
MslI CAYNNNNRTG 2 cut(s) 41, 213
Msp20I TGGCCA 2 cut(s) 55, 419
MspI CCGG 3 cut(s) 282, 864, 896
MspR9I CCNGG 4 cut(s) 27, 68, 73, 865
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 3 cut(s) 27, 68, 73
MwoI GCNNNNNNNGC 4 cut(s) 77, 334, 354, 757
NciI CCSGG 1 cut(s) 865
NdeII GATC 3 cut(s) 306, 872, 924
NlaIII CATG 8 cut(s) 89, 314, 355, 497, 665, 764, 802, 982
NlaIV GGNNCC 3 cut(s) 766, 806, 874
NmeAIII GCCGAG 1 cut(s) 350
NmuCI GTSAC 2 cut(s) 284, 942
OliI CACNNNNGTG 1 cut(s) 41
PagI TCATGA 2 cut(s) 493, 978
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PctI GAATGC 1 cut(s) 781
PfeI GAWTC 4 cut(s) 226, 321, 528, 830
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 4 cut(s) 253, 339, 359, 993
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 43
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 3 cut(s) 25, 66, 71
PspEI GGTNACC 1 cut(s) 942
PspGI CCWGG 3 cut(s) 25, 66, 71
PspN4I GGNNCC 3 cut(s) 766, 806, 874
PspPI GGNCC 1 cut(s) 393
PsuI RGATCY 2 cut(s) 306, 872
RsaI GTAC 2 cut(s) 445, 908
RsaNI GTAC 2 cut(s) 444, 907
RseI CAYNNNNRTG 2 cut(s) 41, 213
SacI GAGCTC 1 cut(s) 330
SalI GTCGAC 2 cut(s) 431, 598
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 4 cut(s) 252, 338, 358, 992
Sau3AI GATC 3 cut(s) 306, 872, 924
Sau96I GGNCC 1 cut(s) 393
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 4 cut(s) 27, 68, 73, 865
SduI GDGCHC 2 cut(s) 330, 807
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 1 cut(s) 130
SmiMI CAYNNNNRTG 2 cut(s) 41, 213
SmlI CTYRAG 3 cut(s) 329, 580, 624
SmoI CTYRAG 3 cut(s) 329, 580, 624
Sse9I AATT 1 cut(s) 398
SsiI CCGC 5 cut(s) 193, 251, 735, 884, 937
SspMI CTAG 1 cut(s) 647
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 4 cut(s) 25, 66, 71, 863
StyI CCWWGG 1 cut(s) 668
TaaI ACNGT 2 cut(s) 565, 685
TaiI ACGT 4 cut(s) 45, 443, 537, 891
TaqI TCGA 9 cut(s) 123, 144, 204, 324, 425, 432, 576, 599, 891
TaqII GACCGA 1 cut(s) 333
TasI AATT 1 cut(s) 398
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 4 cut(s) 226, 321, 528, 830
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TscAI CASTG 2 cut(s) 568, 775
TseFI GTSAC 2 cut(s) 284, 942
TseI GCWGC 3 cut(s) 337, 357, 991
Tsp45I GTSAC 2 cut(s) 284, 942
TspDTI ATGAA 5 cut(s) 109, 627, 664, 967, 995
TspGWI ACGGA 1 cut(s) 924
TspRI CASTG 2 cut(s) 568, 775
Van91I CCANNNNNTGG 1 cut(s) 487
XapI RAATTY 1 cut(s) 398
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XmiI GTMKAC 3 cut(s) 33, 432, 599
XspI CTAG 1 cut(s) 647
ZraI GACGTC 1 cut(s) 889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.