MD12G1030700.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012638
Physical Location & Seq
Reverse (-)
3400709 .. 3404601
3893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1030700.v1.1.491

Sequence Viewer

Length: 948 bp
ATGCAAAGACCAGCAGAGAAAAAAGCAGGCGTCCACACCTACATAAGCCAATGGCCAATCTACTCCTTAGCATGGTCAGTCCGCCATGACAAGCCTTCACGCCTAGCCATAGGCAGCTTCATTGAGGACTACGGCAACAAGGTTGAATTAGTCCAGTTCAACCAAGACACATCCGATTTCGCCACCGATAACCGCCTCACTTTCGACCACCCTTACGCACCAACCAACCTCATGTTCTTCCCCTCCGAGGACACCACGAATCCTGATCTAATCGCCACGTCCGGCGACTACTTAAGGTTGTGGGAGATCCATGATGATAGGATTGAGCTCAAGTCTTTGCTGAATAGCAACAGGAGTAGTGAGTTTAATTCGGCTATAACATCTTTTGATTGGGTAGAGTGTGACACTAGGCGTGTGGCTACTTCTAGTGTGGACACTACCTGCACAATTTGGGACATTGAGAGGGAAGCTGTGGATACTCAACTGGTGGCTCATGATAAAGAAGTGGTGTTTGATTTGAGAAACAAAGAGAGGTCTACCATAGTTTATGAAAACCCTACTCAAGACAGATCTTTGTTGAGGGTAGAATGGAACAAGCATGACCCAAGATTCATTGCCACAGTTGGAATGGACAGCAACAAAGTGGTAATAGTGGACATCAGATTTCCCACATCACCTCTAATGGAGCTCAAAAAACATGGTGCCAGTGTGAATGCAATTTCATGGTCTCCAGGGAAGGGACACCAGATTTGTTCAGTAAGTGATGATTCAAGGGCTATGATTTGGGATGTGGTGAGGCCTGGATTTCAATCGGACAGTGGCGGCGATATGGAGCCGGAGATGTGGTATGGAACGACGGCTCAGATTAATCAAGTGCGTTGGTCTGCTGTAGAGTTGAACGGGATCGGTATTGGCTTTTTGAACAAGTTCCAGCTCTTGAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

316

Amino Acids

35.93

Weight (kDa)

5.15

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 34 - 264 4.6e-10 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 170 - 295 5.9e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 191 - 264 3.4e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 200 - 265 8.8e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 449
AccB1I GGYRCC 1 cut(s) 701
AccI GTMKAC 1 cut(s) 536
AciI CCGC 3 cut(s) 82, 193, 822
AclWI GGATC 2 cut(s) 301, 911
AcoI YGGCCR 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 30
AfiI CCNNNNNNNGG 3 cut(s) 72, 247, 737
AflII CTTAAG 1 cut(s) 292
AgsI TTSAA 7 cut(s) 146, 160, 771, 809, 898, 922, 940
AjiI CACGTC 1 cut(s) 279
AjnI CCWGG 2 cut(s) 730, 799
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 5 cut(s) 117, 328, 470, 688, 934
AluI AGCT 5 cut(s) 117, 328, 470, 688, 934
Alw21I GWGCWC 2 cut(s) 330, 690
Alw26I GTCTC 1 cut(s) 732
AlwI GGATC 2 cut(s) 301, 911
AoxI GGCC 2 cut(s) 53, 797
ApeKI GCWGC 1 cut(s) 114
ArsI GACNNNNNNTTYG 2 cut(s) 732, 764
AseI ATTAAT 1 cut(s) 867
Asp700I GAANNNNTTC 1 cut(s) 926
AsuHPI GGTGA 2 cut(s) 666, 805
BalI TGGCCA 1 cut(s) 55
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 2 cut(s) 330, 690
Bbv12I GWGCWC 2 cut(s) 330, 690
BbvI GCAGC 1 cut(s) 126
BceAI ACGGC 2 cut(s) 148, 873
BciT130I CCWGG 2 cut(s) 732, 801
BciVI GTATCC 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 732
BfaI CTAG 3 cut(s) 104, 408, 426
BfmI CTRYAG 1 cut(s) 888
BfrI CTTAAG 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 449
BfuI GTATCC 1 cut(s) 469
BglII AGATCT 1 cut(s) 569
BisI GCNGC 2 cut(s) 115, 823
BlsI GCNGC 2 cut(s) 116, 824
Bme1390I CCNGG 2 cut(s) 732, 801
BmgBI CACGTC 1 cut(s) 279
BmiI GGNNCC 2 cut(s) 703, 834
BmrFI CCNGG 2 cut(s) 732, 801
BpmI CTGGAG 1 cut(s) 714
Bpu10I CCTNAGC 1 cut(s) 67
BpuEI CTTGAG 2 cut(s) 314, 546
BsaBI GATNNNNATC 1 cut(s) 808
BsaHI GRCGYC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 732
BsaJI CCNNGG 2 cut(s) 246, 731
BsaXI ACNNNNNCTCC 2 cut(s) 830, 860
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 247, 737
Bse1I ACTGG 3 cut(s) 154, 489, 705
Bse3DI GCAATG 1 cut(s) 612
Bse8I GATNNNNATC 1 cut(s) 808
BseBI CCWGG 2 cut(s) 732, 801
BseDI CCNNGG 2 cut(s) 246, 731
BseGI GGATG 2 cut(s) 170, 793
BseJI GATNNNNATC 1 cut(s) 808
BseLI CCNNNNNNNGG 3 cut(s) 72, 247, 737
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 1 cut(s) 875
BseNI ACTGG 3 cut(s) 154, 489, 705
BseXI GCAGC 1 cut(s) 126
BsgI GTGCAG 1 cut(s) 427
BshFI GGCC 2 cut(s) 55, 799
BshNI GGYRCC 1 cut(s) 701
BsiHKAI GWGCWC 2 cut(s) 330, 690
BsiSI CCGG 2 cut(s) 282, 836
BslFI GGGAC 2 cut(s) 467, 753
BslI CCNNNNNNNGG 3 cut(s) 72, 247, 737
BsmAI GTCTC 1 cut(s) 732
BsmFI GGGAC 2 cut(s) 467, 753
BsmI GAATGC 1 cut(s) 718
BsnI GGCC 2 cut(s) 55, 799
Bso31I GGTCTC 1 cut(s) 732
Bsp1286I GDGCHC 2 cut(s) 330, 690
Bsp143I GATC 4 cut(s) 265, 306, 569, 903
BspACI CCGC 3 cut(s) 82, 193, 822
BspANI GGCC 2 cut(s) 55, 799
BspCNI CTCAG 1 cut(s) 874
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 2 cut(s) 703, 834
BspMI ACCTGC 1 cut(s) 449
BspPI GGATC 2 cut(s) 301, 911
BspT107I GGYRCC 1 cut(s) 701
BspTI CTTAAG 1 cut(s) 292
BspTNI GGTCTC 1 cut(s) 732
BsrDI GCAATG 1 cut(s) 612
BsrI ACTGG 3 cut(s) 154, 489, 705
BssECI CCNNGG 2 cut(s) 246, 731
BssMI GATC 4 cut(s) 265, 306, 569, 903
BssNI GRCGYC 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 732, 801
Bst4CI ACNGT 2 cut(s) 622, 818
BstACI GRCGYC 1 cut(s) 30
BstAFI CTTAAG 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 2 cut(s) 67, 861
BstF5I GGATG 2 cut(s) 170, 793
BstKTI GATC 4 cut(s) 268, 309, 572, 906
BstMAI GTCTC 1 cut(s) 732
BstMBI GATC 4 cut(s) 265, 306, 569, 903
BstNI CCWGG 2 cut(s) 732, 801
BstSCI CCNGG 2 cut(s) 730, 799
BstSFI CTRYAG 1 cut(s) 888
BstV1I GCAGC 1 cut(s) 126
BstX2I RGATCY 2 cut(s) 306, 569
BstYI RGATCY 2 cut(s) 306, 569
BsuI GTATCC 1 cut(s) 469
BsuRI GGCC 2 cut(s) 55, 799
BtrI CACGTC 1 cut(s) 279
BtsCI GGATG 2 cut(s) 170, 793
BtsIMutI CAGTG 2 cut(s) 712, 823
BveI ACCTGC 1 cut(s) 449
Cac8I GCNNGC 1 cut(s) 28
CciI TCATGA 1 cut(s) 493
CseI GACGC 1 cut(s) 19
CviAII CATG 8 cut(s) 72, 86, 232, 311, 494, 599, 698, 723
DdeI CTNAG 2 cut(s) 67, 861
DpnI GATC 4 cut(s) 267, 308, 571, 905
DpnII GATC 4 cut(s) 265, 306, 569, 903
EaeI YGGCCR 1 cut(s) 53
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 2 cut(s) 328, 688
Eco147I AGGCCT 1 cut(s) 799
Eco24I GRGCYC 2 cut(s) 330, 690
Eco31I GGTCTC 1 cut(s) 732
Eco53kI GAGCTC 2 cut(s) 328, 688
EcoICRI GAGCTC 2 cut(s) 328, 688
EcoRII CCWGG 2 cut(s) 730, 799
EcoT38I GRGCYC 2 cut(s) 330, 690
FaeI CATG 8 cut(s) 75, 89, 235, 314, 497, 602, 701, 726
FaqI GGGAC 2 cut(s) 467, 753
FatI CATG 8 cut(s) 71, 85, 231, 310, 493, 598, 697, 722
FblI GTMKAC 1 cut(s) 536
Fnu4HI GCNGC 2 cut(s) 115, 823
FokI GGATG 2 cut(s) 157, 800
FriOI GRGCYC 2 cut(s) 330, 690
Fsp4HI GCNGC 2 cut(s) 115, 823
FspBI CTAG 3 cut(s) 104, 408, 426
GluI GCNGC 2 cut(s) 115, 823
GsuI CTGGAG 1 cut(s) 714
HaeIII GGCC 2 cut(s) 55, 799
HapII CCGG 2 cut(s) 282, 836
HgaI GACGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 8 cut(s) 75, 89, 235, 314, 497, 602, 701, 726
HinfI GANTC 3 cut(s) 259, 609, 767
HpaII CCGG 2 cut(s) 282, 836
HphI GGTGA 2 cut(s) 666, 805
Hpy166II GTNNAC 4 cut(s) 34, 433, 537, 655
Hpy188I TCNGA 5 cut(s) 175, 247, 662, 814, 864
Hpy188III TCNNGA 4 cut(s) 263, 494, 563, 937
Hpy8I GTNNAC 4 cut(s) 34, 433, 537, 655
Hpy99I CGWCG 1 cut(s) 859
HpyAV CCTTC 3 cut(s) 105, 730, 934
HpyCH4III ACNGT 2 cut(s) 622, 818
HpyCH4IV ACGT 1 cut(s) 278
HpyCH4V TGCA 3 cut(s) 4, 444, 716
HpyF3I CTNAG 2 cut(s) 67, 861
HpySE526I ACGT 1 cut(s) 278
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 8 cut(s) 75, 89, 235, 314, 497, 602, 701, 726
Kzo9I GATC 4 cut(s) 265, 306, 569, 903
LmnI GCTCC 2 cut(s) 685, 832
Lsp1109I GCAGC 1 cut(s) 126
MaeI CTAG 3 cut(s) 104, 408, 426
MaeII ACGT 1 cut(s) 278
MaeIII GTNAC 1 cut(s) 401
MalI GATC 4 cut(s) 267, 308, 571, 905
MboI GATC 4 cut(s) 265, 306, 569, 903
MboII GAAGA 1 cut(s) 229
MflI RGATCY 2 cut(s) 306, 569
MhlI GDGCHC 2 cut(s) 330, 690
MlsI TGGCCA 1 cut(s) 55
MluCI AATT 4 cut(s) 146, 367, 447, 717
MluNI TGGCCA 1 cut(s) 55
MmeI TCCRAC 1 cut(s) 604
Mox20I TGGCCA 1 cut(s) 55
MroXI GAANNNNTTC 1 cut(s) 926
MscI TGGCCA 1 cut(s) 55
MseI TTAA 4 cut(s) 293, 366, 867, 946
Msp20I TGGCCA 1 cut(s) 55
MspCI CTTAAG 1 cut(s) 292
MspI CCGG 2 cut(s) 282, 836
MspR9I CCNGG 2 cut(s) 732, 801
Mva1269I GAATGC 1 cut(s) 718
MvaI CCWGG 2 cut(s) 732, 801
NdeII GATC 4 cut(s) 265, 306, 569, 903
NlaIII CATG 8 cut(s) 75, 89, 235, 314, 497, 602, 701, 726
NlaIV GGNNCC 2 cut(s) 703, 834
NmuCI GTSAC 1 cut(s) 401
PagI TCATGA 1 cut(s) 493
PceI AGGCCT 1 cut(s) 799
PctI GAATGC 1 cut(s) 718
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 3 cut(s) 259, 609, 767
PkrI GCNGC 2 cut(s) 116, 824
PshBI ATTAAT 1 cut(s) 867
Psp124BI GAGCTC 2 cut(s) 330, 690
Psp6I CCWGG 2 cut(s) 730, 799
PspGI CCWGG 2 cut(s) 730, 799
PspN4I GGNNCC 2 cut(s) 703, 834
PsuI RGATCY 2 cut(s) 306, 569
SacI GAGCTC 2 cut(s) 330, 690
SaqAI TTAA 4 cut(s) 293, 366, 867, 946
SatI GCNGC 2 cut(s) 115, 823
Sau3AI GATC 4 cut(s) 265, 306, 569, 903
ScrFI CCNGG 2 cut(s) 732, 801
SduI GDGCHC 2 cut(s) 330, 690
SfcI CTRYAG 1 cut(s) 888
SmlI CTYRAG 3 cut(s) 292, 329, 561
SmoI CTYRAG 3 cut(s) 292, 329, 561
Sse9I AATT 4 cut(s) 146, 367, 447, 717
SseBI AGGCCT 1 cut(s) 799
SsiI CCGC 3 cut(s) 82, 193, 822
SspMI CTAG 3 cut(s) 104, 408, 426
SstI GAGCTC 2 cut(s) 330, 690
StuI AGGCCT 1 cut(s) 799
StyD4I CCNGG 2 cut(s) 730, 799
TaaI ACNGT 2 cut(s) 622, 818
TaiI ACGT 1 cut(s) 281
TaqI TCGA 1 cut(s) 204
TasI AATT 4 cut(s) 146, 367, 447, 717
TauI GCSGC 1 cut(s) 825
TfiI GAWTC 3 cut(s) 259, 609, 767
Tru1I TTAA 4 cut(s) 293, 366, 867, 946
Tru9I TTAA 4 cut(s) 293, 366, 867, 946
TscAI CASTG 2 cut(s) 712, 823
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 1 cut(s) 114
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 4 cut(s) 109, 564, 601, 711
TspRI CASTG 2 cut(s) 712, 823
Vha464I CTTAAG 1 cut(s) 292
VspI ATTAAT 1 cut(s) 867
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XmiI GTMKAC 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 3 cut(s) 104, 408, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.