pycom14g02810

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012638
Physical Location & Seq
Reverse (-)
2137812 .. 2139400
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g02810.1

Sequence Viewer

Length: 1011 bp
ATGCAAAGTCCAGCAGAGAAAAAAGCAGGCGTCCACACCTACGTAAGTCAATGGCCAATCTCCTCCTTAGCATGGTCAGTCCGCCGTGACAAACCTTCACGCCTAGCCATTGGCAGCTTCATTGAGGACTACAGCAACAAGGTTGAACTAGTCCAGTTCAACCGTGACACATCCGACTTCGCCACCGATAGCCGCCTCATTTTCGACCACCCTTACGCACCAACCAACCTCATGTTCTTCCCCTCTGAGGACACCGCGAATCCTGATCTAATCGCCACGTCCGGTGACTACTTAAGGCTATGGGAGATCCATGATGATAGGATTGAGCTCAAGTCTCTTCTGAATAGCAACATGAGTAGTGAGTTTAATTCGGCTATAACGTCTTTTGATTGGGCTGAGTTTGACACTAGGCGTGTGGCTACGTCGAGTGTGGACACTACATGCACGATTTGGGACATTGTGAGGGAAGTTGTGGATACTCAGTTGGTGGCTCATGACAAAGAAGTGTATGACATTTCTTGGGGTGGCTTCAATGTGTTTGCTTCAGCTTCTGGTGATGGCACTGTCAGGGTGTTTGATTTGAGAAACAAAGAGAGGTCTACGATAGTTTATGAAAACCCTACTCAAGAAAGATCTTTGTTGAGGCTAGAATGGAACAAACATGACCCAAGATTCATTGCCACAGTTGGAATGGACAGCAACAAAGTGGTGATATTGGACATCAGATTTCCCACATCACCTCTGATGGAGCTCAAAAAACATGGTGCCAGTGTGAATGCCATTTCATGGTCTCCAGGGAAAGGACACCAATTATGTTCGGTGAGTGATGATTCAAGGGCTCTGATTTGGGATGTGGTGAGACATGGATTTCAGTCCAATGGCGGCGGCGATGCGGAGCCGGATATGTGGTATGGAACGGCAGCTCAGATTAATCAAGTACGTTGGTCTGCTTTAGAGTTGAACTGGATTGGTATTGGCTTTTTGAACAAATTGCAGCTCTTGAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

337

Amino Acids

38.02

Weight (kDa)

5.23

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 34 - 283 5.8e-08 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 99 - 286 2.6e-20 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 105 - 205 4.9e-10 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 115 - 286 6.1e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 123 - 202 1.2e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 125 - 242 3.5e-12 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 125 - 200 2.1e-09 WDR5 beta-propeller domain
WD40_Gbeta PF25391 134 - 197 3.9e-06 G protein beta WD-40 repeat protein
Beta-prop_RIG_2nd PF23775 184 - 286 5.6e-06 RIG second beta-propeller
Beta-prop_THOC3 PF25174 212 - 285 5.2e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 764
AccB7I CCANNNNNTGG 1 cut(s) 786
AccI GTMKAC 1 cut(s) 599
AccII CGCG 1 cut(s) 257
AciI CCGC 6 cut(s) 82, 193, 255, 882, 885, 893
AclWI GGATC 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 53
AcuI CTGAAG 1 cut(s) 528
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 1 cut(s) 939
AfiI CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
AflII CTTAAG 1 cut(s) 292
AgsI TTSAA 7 cut(s) 146, 160, 532, 834, 961, 985, 1003
AhlI ACTAGT 1 cut(s) 148
AjiI CACGTC 1 cut(s) 279
AjnI CCWGG 1 cut(s) 793
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 6 cut(s) 117, 328, 548, 751, 923, 997
AluI AGCT 6 cut(s) 117, 328, 548, 751, 923, 997
Alw21I GWGCWC 2 cut(s) 330, 753
Alw26I GTCTC 3 cut(s) 339, 795, 853
AlwI GGATC 1 cut(s) 301
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 1 cut(s) 53
ApeKI GCWGC 3 cut(s) 114, 920, 994
AseI ATTAAT 1 cut(s) 930
AsuHPI GGTGA 6 cut(s) 296, 566, 721, 729, 832, 868
BalI TGGCCA 1 cut(s) 55
BanI GGYRCC 1 cut(s) 764
BanII GRGCYC 3 cut(s) 330, 753, 841
Bbv12I GWGCWC 2 cut(s) 330, 753
BbvI GCAGC 3 cut(s) 126, 932, 1006
BccI CCATC 2 cut(s) 551, 739
BceAI ACGGC 2 cut(s) 69, 933
BciT130I CCWGG 1 cut(s) 795
BciVI GTATCC 1 cut(s) 469
BcoDI GTCTC 3 cut(s) 339, 795, 853
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 4 cut(s) 104, 149, 408, 647
BfmI CTRYAG 1 cut(s) 130
BfrI CTTAAG 1 cut(s) 292
BfuI GTATCC 1 cut(s) 469
BglII AGATCT 1 cut(s) 632
BisI GCNGC 6 cut(s) 115, 193, 883, 886, 921, 995
BlsI GCNGC 6 cut(s) 116, 194, 884, 887, 922, 996
Bme1390I CCNGG 1 cut(s) 795
BmgBI CACGTC 1 cut(s) 279
BmiI GGNNCC 2 cut(s) 766, 897
BmrFI CCNGG 1 cut(s) 795
BmsI GCATC 1 cut(s) 880
BpmI CTGGAG 1 cut(s) 777
Bpu10I CCTNAGC 1 cut(s) 67
BpuEI CTTGAG 2 cut(s) 314, 609
BsaAI YACGTR 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 795
BsaJI CCNNGG 1 cut(s) 794
BsaWI WCCGGW 1 cut(s) 281
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
Bse1I ACTGG 3 cut(s) 154, 768, 968
Bse3DI GCAATG 1 cut(s) 675
BseBI CCWGG 1 cut(s) 795
BseDI CCNNGG 1 cut(s) 794
BseGI GGATG 2 cut(s) 170, 856
BseLI CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 4 cut(s) 237, 387, 494, 938
BseNI ACTGG 3 cut(s) 154, 768, 968
BseRI GAGGAG 1 cut(s) 52
BseXI GCAGC 3 cut(s) 126, 932, 1006
Bsh1236I CGCG 1 cut(s) 257
BshFI GGCC 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 764
BsiHKAI GWGCWC 2 cut(s) 330, 753
BsiSI CCGG 2 cut(s) 282, 899
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
BsmAI GTCTC 3 cut(s) 339, 795, 853
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 1 cut(s) 55
Bso31I GGTCTC 1 cut(s) 795
Bsp1286I GDGCHC 3 cut(s) 330, 753, 841
Bsp143I GATC 3 cut(s) 265, 306, 632
BspACI CCGC 6 cut(s) 82, 193, 255, 882, 885, 893
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 4 cut(s) 238, 388, 493, 937
BspFNI CGCG 1 cut(s) 257
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 2 cut(s) 766, 897
BspPI GGATC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 764
BspTI CTTAAG 1 cut(s) 292
BspTNI GGTCTC 1 cut(s) 795
BsrDI GCAATG 1 cut(s) 675
BsrI ACTGG 3 cut(s) 154, 768, 968
BssECI CCNNGG 1 cut(s) 794
BssMI GATC 3 cut(s) 265, 306, 632
BssNI GRCGYC 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 795
Bst4CI ACNGT 3 cut(s) 164, 565, 685
Bst6I CTCTTC 1 cut(s) 342
BstACI GRCGYC 1 cut(s) 30
BstAFI CTTAAG 1 cut(s) 292
BstBAI YACGTR 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 5 cut(s) 67, 246, 396, 480, 924
BstF5I GGATG 2 cut(s) 170, 856
BstFNI CGCG 1 cut(s) 257
BstKTI GATC 3 cut(s) 268, 309, 635
BstMAI GTCTC 3 cut(s) 339, 795, 853
BstMBI GATC 3 cut(s) 265, 306, 632
BstNI CCWGG 1 cut(s) 795
BstNSI RCATGY 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 793
BstSFI CTRYAG 1 cut(s) 130
BstSNI TACGTA 1 cut(s) 43
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 3 cut(s) 126, 932, 1006
BstX2I RGATCY 2 cut(s) 306, 632
BstYI RGATCY 2 cut(s) 306, 632
BsuI GTATCC 1 cut(s) 469
BsuRI GGCC 1 cut(s) 55
BtgZI GCGATG 1 cut(s) 903
BtrI CACGTC 1 cut(s) 279
BtsCI GGATG 2 cut(s) 170, 856
BtsIMutI CAGTG 2 cut(s) 561, 775
Cac8I GCNNGC 1 cut(s) 28
CaiI CAGNNNCTG 1 cut(s) 551
CciI TCATGA 1 cut(s) 493
CseI GACGC 1 cut(s) 19
Csp6I GTAC 1 cut(s) 938
CviQI GTAC 1 cut(s) 938
DdeI CTNAG 5 cut(s) 67, 246, 396, 480, 924
DpnI GATC 3 cut(s) 267, 308, 634
DpnII GATC 3 cut(s) 265, 306, 632
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 2 cut(s) 328, 751
Eco105I TACGTA 1 cut(s) 43
Eco24I GRGCYC 3 cut(s) 330, 753, 841
Eco31I GGTCTC 1 cut(s) 795
Eco53kI GAGCTC 2 cut(s) 328, 751
Eco57I CTGAAG 1 cut(s) 528
EcoICRI GAGCTC 2 cut(s) 328, 751
EcoRII CCWGG 1 cut(s) 793
EcoT38I GRGCYC 3 cut(s) 330, 753, 841
FaqI GGGAC 1 cut(s) 467
FblI GTMKAC 1 cut(s) 599
Fnu4HI GCNGC 6 cut(s) 115, 193, 883, 886, 921, 995
FokI GGATG 2 cut(s) 157, 863
FriOI GRGCYC 3 cut(s) 330, 753, 841
Fsp4HI GCNGC 6 cut(s) 115, 193, 883, 886, 921, 995
FspBI CTAG 4 cut(s) 104, 149, 408, 647
GluI GCNGC 6 cut(s) 115, 193, 883, 886, 921, 995
GsuI CTGGAG 1 cut(s) 777
HaeIII GGCC 1 cut(s) 55
HapII CCGG 2 cut(s) 282, 899
HgaI GACGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 30
HinfI GANTC 3 cut(s) 259, 672, 830
HpaII CCGG 2 cut(s) 282, 899
HphI GGTGA 6 cut(s) 296, 566, 721, 729, 832, 868
Hpy166II GTNNAC 3 cut(s) 34, 433, 600
Hpy188I TCNGA 7 cut(s) 175, 247, 342, 725, 744, 843, 927
Hpy188III TCNNGA 4 cut(s) 263, 494, 626, 1000
Hpy8I GTNNAC 3 cut(s) 34, 433, 600
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 2 cut(s) 105, 997
HpyCH4III ACNGT 3 cut(s) 164, 565, 685
HpyCH4IV ACGT 5 cut(s) 42, 278, 380, 422, 940
HpyCH4V TGCA 3 cut(s) 4, 444, 994
HpyF3I CTNAG 5 cut(s) 67, 246, 396, 480, 924
HpySE526I ACGT 5 cut(s) 42, 278, 380, 422, 940
Hsp92I GRCGYC 1 cut(s) 30
Kzo9I GATC 3 cut(s) 265, 306, 632
LmnI GCTCC 2 cut(s) 748, 895
Lsp1109I GCAGC 3 cut(s) 126, 932, 1006
LweI GCATC 1 cut(s) 880
MaeI CTAG 4 cut(s) 104, 149, 408, 647
MaeII ACGT 5 cut(s) 42, 278, 380, 422, 940
MaeIII GTNAC 3 cut(s) 86, 164, 284
MalI GATC 3 cut(s) 267, 308, 634
MboI GATC 3 cut(s) 265, 306, 632
MboII GAAGA 2 cut(s) 229, 329
MflI RGATCY 2 cut(s) 306, 632
MhlI GDGCHC 3 cut(s) 330, 753, 841
MlsI TGGCCA 1 cut(s) 55
MluCI AATT 3 cut(s) 367, 809, 989
MluNI TGGCCA 1 cut(s) 55
MmeI TCCRAC 2 cut(s) 198, 667
Mox20I TGGCCA 1 cut(s) 55
MscI TGGCCA 1 cut(s) 55
MseI TTAA 4 cut(s) 293, 366, 930, 1009
Msp20I TGGCCA 1 cut(s) 55
MspCI CTTAAG 1 cut(s) 292
MspI CCGG 2 cut(s) 282, 899
MspR9I CCNGG 1 cut(s) 795
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 1 cut(s) 795
MvnI CGCG 1 cut(s) 257
NdeII GATC 3 cut(s) 265, 306, 632
NlaIV GGNNCC 2 cut(s) 766, 897
NmuCI GTSAC 3 cut(s) 86, 164, 284
NspI RCATGY 1 cut(s) 444
PagI TCATGA 1 cut(s) 493
PcsI WCGNNNNNNNCGW 1 cut(s) 377
PctI GAATGC 1 cut(s) 781
PfeI GAWTC 3 cut(s) 259, 672, 830
PflMI CCANNNNNTGG 1 cut(s) 786
PkrI GCNGC 6 cut(s) 116, 194, 884, 887, 922, 996
Ppu21I YACGTR 1 cut(s) 43
PshBI ATTAAT 1 cut(s) 930
Psp124BI GAGCTC 2 cut(s) 330, 753
Psp6I CCWGG 1 cut(s) 793
PspGI CCWGG 1 cut(s) 793
PspN4I GGNNCC 2 cut(s) 766, 897
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 2 cut(s) 306, 632
RsaI GTAC 1 cut(s) 939
RsaNI GTAC 1 cut(s) 938
SacI GAGCTC 2 cut(s) 330, 753
SaqAI TTAA 4 cut(s) 293, 366, 930, 1009
SatI GCNGC 6 cut(s) 115, 193, 883, 886, 921, 995
Sau3AI GATC 3 cut(s) 265, 306, 632
ScrFI CCNGG 1 cut(s) 795
SduI GDGCHC 3 cut(s) 330, 753, 841
SfaNI GCATC 1 cut(s) 880
SfcI CTRYAG 1 cut(s) 130
SmlI CTYRAG 3 cut(s) 292, 329, 624
SmoI CTYRAG 3 cut(s) 292, 329, 624
SnaBI TACGTA 1 cut(s) 43
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 3 cut(s) 367, 809, 989
SsiI CCGC 6 cut(s) 82, 193, 255, 882, 885, 893
SspMI CTAG 4 cut(s) 104, 149, 408, 647
SstI GAGCTC 2 cut(s) 330, 753
StyD4I CCNGG 1 cut(s) 793
TaaI ACNGT 3 cut(s) 164, 565, 685
TaiI ACGT 5 cut(s) 45, 281, 383, 425, 943
TaqI TCGA 2 cut(s) 204, 425
TasI AATT 3 cut(s) 367, 809, 989
TauI GCSGC 3 cut(s) 195, 885, 888
TfiI GAWTC 3 cut(s) 259, 672, 830
Tru1I TTAA 4 cut(s) 293, 366, 930, 1009
Tru9I TTAA 4 cut(s) 293, 366, 930, 1009
TscAI CASTG 2 cut(s) 568, 775
TseFI GTSAC 3 cut(s) 86, 164, 284
TseI GCWGC 3 cut(s) 114, 920, 994
Tsp45I GTSAC 3 cut(s) 86, 164, 284
TspDTI ATGAA 4 cut(s) 109, 627, 664, 774
TspRI CASTG 2 cut(s) 568, 775
Van91I CCANNNNNTGG 1 cut(s) 786
Vha464I CTTAAG 1 cut(s) 292
VspI ATTAAT 1 cut(s) 930
XceI RCATGY 1 cut(s) 444
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XmiI GTMKAC 1 cut(s) 599
XspI CTAG 4 cut(s) 104, 149, 408, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.