MD14G1031200.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012638
Physical Location & Seq
Reverse (-)
2785170 .. 2788026
2857 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1031200.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGCAAAGTCCAGCAGAGAAAAAAGCAGGCGTCCACACCTACGTAAGTCAATGGCCAATCTCCTCCTTAGCCTGGTCAGTCCGCCATGACAAACCTTCACGCCTAGCCATTGGCAGCTTCATTGAGGACTACAGCAACAAGGTTGAACTAGTCCAGTTCAACCGTGACACATCCGACTTCGCCACCGATAGCCGCCTCATTTTCGACCACCCTTACGCACCAACCAACCTCATGTTCTTCCCCTCTGAGGACACCGCGAATCCTGATCTAATCGCCACGTCCGGTGACTACTTAAGGCTATGGGAGATCCATGATGATAGGATTGAGCTCAAGTCTCTGCTGAATAGCAACATGAGCAGTGAGTTTAATTCGGCCATAACGTCTTTTGACTGGGCTGAGTTTGACACTAGGCGAGTGGCTACGTCGAGTGTGGACACTACATGCACAATTTGGGATATTGAGAGGGAAGTTGTGGACACTCAGTTGGTGGCTCATGACAAAGAAGTGTATGACATTTCTTGGGGTGGCTTCAATGTGTTTGCTTCAGCTTCTGGTGATGGCACTGTCAGGGTGTTTGATTTGAGAAACAAAGAGAGGTCTACCATAGTTTATGAAAACCCTACTCAAGAAAGATCTTTGTTGAGGCTAGAATGGAACAAACATGACCCAAGATTCATTGCCACAGTTGGAATGGACAGCAACAAAGTGGTGATATTGGACATCAGATTTCCCACATTACCTCTGATGGAGCTCAAAAAACATGGTGCCAGTGTGAATGCCATTTCATGGTCTCCAGGGAAAGGACACCAATTATGTTCGGTGAGTGATGATTCAAGGGCTTTAATTTGGGATGTGGTGAGACCTGGATTTCAGTCCAATGGCGGCGGCGATGCGGAGCCGGAGATGTGGTATGGAACGACAGCTCAGATTAATCAAGTGCGTTGGTCTGCTCTAGAAATGAACTGGATTGGTATTGGCTTTTTGAACAAGTTGCAGCTCTTGAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

337

Amino Acids

38.07

Weight (kDa)

5.08

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 29 - 283 1.9e-08 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 99 - 316 1.8e-20 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 105 - 208 2e-09 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 115 - 285 3.9e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 123 - 202 3.7e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 125 - 242 3.9e-13 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 125 - 202 4.6e-10 WDR5 beta-propeller domain
WD40_Gbeta PF25391 134 - 197 3.9e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 136 - 200 7.3e-06 WDR55
Beta-prop_RIG_2nd PF23775 185 - 287 4.8e-06 RIG second beta-propeller
Beta-prop_THOC3 PF25174 212 - 285 8.9e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 764
AccB7I CCANNNNNTGG 1 cut(s) 786
AccI GTMKAC 1 cut(s) 599
AccII CGCG 1 cut(s) 257
AciI CCGC 6 cut(s) 82, 193, 255, 882, 885, 893
AclWI GGATC 1 cut(s) 301
AcoI YGGCCR 2 cut(s) 53, 372
AcuI CTGAAG 1 cut(s) 528
AcyI GRCGYC 1 cut(s) 30
AfiI CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
AflII CTTAAG 1 cut(s) 292
AgsI TTSAA 6 cut(s) 146, 160, 532, 834, 985, 1003
AhlI ACTAGT 1 cut(s) 148
AjiI CACGTC 1 cut(s) 279
AjnI CCWGG 3 cut(s) 71, 793, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 6 cut(s) 117, 328, 548, 751, 923, 997
AluI AGCT 6 cut(s) 117, 328, 548, 751, 923, 997
Alw21I GWGCWC 2 cut(s) 330, 753
Alw26I GTCTC 3 cut(s) 339, 795, 853
AlwI GGATC 1 cut(s) 301
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 2 cut(s) 53, 372
ApeKI GCWGC 2 cut(s) 114, 994
AseI ATTAAT 1 cut(s) 930
AsuHPI GGTGA 5 cut(s) 296, 566, 721, 832, 868
BalI TGGCCA 1 cut(s) 55
BanI GGYRCC 1 cut(s) 764
BanII GRGCYC 2 cut(s) 330, 753
Bbv12I GWGCWC 2 cut(s) 330, 753
BbvI GCAGC 2 cut(s) 126, 1006
BccI CCATC 2 cut(s) 551, 739
BciT130I CCWGG 3 cut(s) 73, 795, 864
BcoDI GTCTC 3 cut(s) 339, 795, 853
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 5 cut(s) 104, 149, 408, 647, 953
BfmI CTRYAG 1 cut(s) 130
BfrI CTTAAG 1 cut(s) 292
BglII AGATCT 1 cut(s) 632
BisI GCNGC 5 cut(s) 115, 193, 883, 886, 995
BlsI GCNGC 5 cut(s) 116, 194, 884, 887, 996
Bme1390I CCNGG 3 cut(s) 73, 795, 864
BmgBI CACGTC 1 cut(s) 279
BmiI GGNNCC 2 cut(s) 766, 897
BmrFI CCNGG 3 cut(s) 73, 795, 864
BmrI ACTGGG 1 cut(s) 400
BmsI GCATC 1 cut(s) 880
BmuI ACTGGG 1 cut(s) 400
BpmI CTGGAG 1 cut(s) 777
Bpu10I CCTNAGC 1 cut(s) 67
BpuEI CTTGAG 2 cut(s) 314, 609
BsaAI YACGTR 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 30
BsaI GGTCTC 2 cut(s) 795, 853
BsaJI CCNNGG 1 cut(s) 794
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 893, 923
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
Bse1I ACTGG 4 cut(s) 154, 395, 768, 968
Bse3DI GCAATG 1 cut(s) 675
BseBI CCWGG 3 cut(s) 73, 795, 864
BseDI CCNNGG 1 cut(s) 794
BseGI GGATG 2 cut(s) 170, 856
BseLI CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 4 cut(s) 237, 387, 494, 938
BseNI ACTGG 4 cut(s) 154, 395, 768, 968
BseRI GAGGAG 1 cut(s) 52
BseXI GCAGC 2 cut(s) 126, 1006
Bsh1236I CGCG 1 cut(s) 257
BshFI GGCC 2 cut(s) 55, 374
BshNI GGYRCC 1 cut(s) 764
BsiHKAI GWGCWC 2 cut(s) 330, 753
BsiSI CCGG 2 cut(s) 282, 899
BslI CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
BsmAI GTCTC 3 cut(s) 339, 795, 853
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 2 cut(s) 55, 374
Bso31I GGTCTC 2 cut(s) 795, 853
Bsp1286I GDGCHC 2 cut(s) 330, 753
Bsp143I GATC 3 cut(s) 265, 306, 632
BspACI CCGC 6 cut(s) 82, 193, 255, 882, 885, 893
BspANI GGCC 2 cut(s) 55, 374
BspCNI CTCAG 4 cut(s) 238, 388, 493, 937
BspFNI CGCG 1 cut(s) 257
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 2 cut(s) 766, 897
BspPI GGATC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 764
BspTI CTTAAG 1 cut(s) 292
BspTNI GGTCTC 2 cut(s) 795, 853
BsrDI GCAATG 1 cut(s) 675
BsrI ACTGG 4 cut(s) 154, 395, 768, 968
BssECI CCNNGG 1 cut(s) 794
BssMI GATC 3 cut(s) 265, 306, 632
BssNI GRCGYC 1 cut(s) 30
Bst2UI CCWGG 3 cut(s) 73, 795, 864
Bst4CI ACNGT 3 cut(s) 164, 565, 685
BstACI GRCGYC 1 cut(s) 30
BstAFI CTTAAG 1 cut(s) 292
BstBAI YACGTR 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 5 cut(s) 67, 246, 396, 480, 924
BstF5I GGATG 2 cut(s) 170, 856
BstFNI CGCG 1 cut(s) 257
BstKTI GATC 3 cut(s) 268, 309, 635
BstMAI GTCTC 3 cut(s) 339, 795, 853
BstMBI GATC 3 cut(s) 265, 306, 632
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstNI CCWGG 3 cut(s) 73, 795, 864
BstNSI RCATGY 1 cut(s) 444
BstSCI CCNGG 3 cut(s) 71, 793, 862
BstSFI CTRYAG 1 cut(s) 130
BstSNI TACGTA 1 cut(s) 43
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 2 cut(s) 126, 1006
BstX2I RGATCY 2 cut(s) 306, 632
BstYI RGATCY 2 cut(s) 306, 632
BsuRI GGCC 2 cut(s) 55, 374
BtgZI GCGATG 1 cut(s) 903
BtrI CACGTC 1 cut(s) 279
BtsCI GGATG 2 cut(s) 170, 856
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 3 cut(s) 364, 561, 775
Cac8I GCNNGC 1 cut(s) 28
CaiI CAGNNNCTG 1 cut(s) 551
CciI TCATGA 1 cut(s) 493
CseI GACGC 1 cut(s) 19
CviAII CATG 9 cut(s) 86, 232, 311, 352, 441, 494, 662, 761, 786
DdeI CTNAG 5 cut(s) 67, 246, 396, 480, 924
DpnI GATC 3 cut(s) 267, 308, 634
DpnII GATC 3 cut(s) 265, 306, 632
EaeI YGGCCR 2 cut(s) 53, 372
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 2 cut(s) 328, 751
Eco105I TACGTA 1 cut(s) 43
Eco24I GRGCYC 2 cut(s) 330, 753
Eco31I GGTCTC 2 cut(s) 795, 853
Eco53kI GAGCTC 2 cut(s) 328, 751
Eco57I CTGAAG 1 cut(s) 528
EcoICRI GAGCTC 2 cut(s) 328, 751
EcoRII CCWGG 3 cut(s) 71, 793, 862
EcoT38I GRGCYC 2 cut(s) 330, 753
FaeI CATG 9 cut(s) 89, 235, 314, 355, 444, 497, 665, 764, 789
FatI CATG 9 cut(s) 85, 231, 310, 351, 440, 493, 661, 760, 785
FblI GTMKAC 1 cut(s) 599
Fnu4HI GCNGC 5 cut(s) 115, 193, 883, 886, 995
FokI GGATG 2 cut(s) 157, 863
FriOI GRGCYC 2 cut(s) 330, 753
Fsp4HI GCNGC 5 cut(s) 115, 193, 883, 886, 995
FspBI CTAG 5 cut(s) 104, 149, 408, 647, 953
GluI GCNGC 5 cut(s) 115, 193, 883, 886, 995
GsuI CTGGAG 1 cut(s) 777
HaeIII GGCC 2 cut(s) 55, 374
HapII CCGG 2 cut(s) 282, 899
HgaI GACGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 9 cut(s) 89, 235, 314, 355, 444, 497, 665, 764, 789
HinfI GANTC 3 cut(s) 259, 672, 830
HpaII CCGG 2 cut(s) 282, 899
HphI GGTGA 5 cut(s) 296, 566, 721, 832, 868
Hpy166II GTNNAC 4 cut(s) 34, 433, 475, 600
Hpy188I TCNGA 5 cut(s) 175, 247, 725, 744, 927
Hpy188III TCNNGA 5 cut(s) 263, 494, 626, 953, 1000
Hpy8I GTNNAC 4 cut(s) 34, 433, 475, 600
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 2 cut(s) 105, 997
HpyCH4III ACNGT 3 cut(s) 164, 565, 685
HpyCH4IV ACGT 4 cut(s) 42, 278, 380, 422
HpyCH4V TGCA 3 cut(s) 4, 444, 994
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 5 cut(s) 67, 246, 396, 480, 924
HpySE526I ACGT 4 cut(s) 42, 278, 380, 422
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 9 cut(s) 89, 235, 314, 355, 444, 497, 665, 764, 789
Kzo9I GATC 3 cut(s) 265, 306, 632
LmnI GCTCC 2 cut(s) 748, 895
Lsp1109I GCAGC 2 cut(s) 126, 1006
LweI GCATC 1 cut(s) 880
MaeI CTAG 5 cut(s) 104, 149, 408, 647, 953
MaeII ACGT 4 cut(s) 42, 278, 380, 422
MaeIII GTNAC 2 cut(s) 164, 284
MalI GATC 3 cut(s) 267, 308, 634
MboI GATC 3 cut(s) 265, 306, 632
MboII GAAGA 1 cut(s) 229
MflI RGATCY 2 cut(s) 306, 632
MhlI GDGCHC 2 cut(s) 330, 753
MlsI TGGCCA 1 cut(s) 55
MluCI AATT 4 cut(s) 367, 447, 809, 843
MluNI TGGCCA 1 cut(s) 55
MmeI TCCRAC 2 cut(s) 198, 667
Mox20I TGGCCA 1 cut(s) 55
MscI TGGCCA 1 cut(s) 55
MseI TTAA 5 cut(s) 293, 366, 842, 930, 1009
Msp20I TGGCCA 1 cut(s) 55
MspCI CTTAAG 1 cut(s) 292
MspI CCGG 2 cut(s) 282, 899
MspR9I CCNGG 3 cut(s) 73, 795, 864
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 3 cut(s) 73, 795, 864
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 354
NdeII GATC 3 cut(s) 265, 306, 632
NlaIII CATG 9 cut(s) 89, 235, 314, 355, 444, 497, 665, 764, 789
NlaIV GGNNCC 2 cut(s) 766, 897
NmuCI GTSAC 2 cut(s) 164, 284
NspI RCATGY 1 cut(s) 444
PagI TCATGA 1 cut(s) 493
PcsI WCGNNNNNNNCGW 1 cut(s) 377
PctI GAATGC 1 cut(s) 781
PfeI GAWTC 3 cut(s) 259, 672, 830
PflMI CCANNNNNTGG 1 cut(s) 786
PkrI GCNGC 5 cut(s) 116, 194, 884, 887, 996
Ppu21I YACGTR 1 cut(s) 43
PshBI ATTAAT 1 cut(s) 930
Psp124BI GAGCTC 2 cut(s) 330, 753
Psp6I CCWGG 3 cut(s) 71, 793, 862
PspGI CCWGG 3 cut(s) 71, 793, 862
PspN4I GGNNCC 2 cut(s) 766, 897
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 2 cut(s) 306, 632
SacI GAGCTC 2 cut(s) 330, 753
SaqAI TTAA 5 cut(s) 293, 366, 842, 930, 1009
SatI GCNGC 5 cut(s) 115, 193, 883, 886, 995
Sau3AI GATC 3 cut(s) 265, 306, 632
ScrFI CCNGG 3 cut(s) 73, 795, 864
SduI GDGCHC 2 cut(s) 330, 753
SfaNI GCATC 1 cut(s) 880
SfcI CTRYAG 1 cut(s) 130
SmlI CTYRAG 3 cut(s) 292, 329, 624
SmoI CTYRAG 3 cut(s) 292, 329, 624
SnaBI TACGTA 1 cut(s) 43
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 4 cut(s) 367, 447, 809, 843
SsiI CCGC 6 cut(s) 82, 193, 255, 882, 885, 893
SspMI CTAG 5 cut(s) 104, 149, 408, 647, 953
SstI GAGCTC 2 cut(s) 330, 753
StyD4I CCNGG 3 cut(s) 71, 793, 862
TaaI ACNGT 3 cut(s) 164, 565, 685
TaiI ACGT 4 cut(s) 45, 281, 383, 425
TaqI TCGA 2 cut(s) 204, 425
TasI AATT 4 cut(s) 367, 447, 809, 843
TauI GCSGC 3 cut(s) 195, 885, 888
TfiI GAWTC 3 cut(s) 259, 672, 830
Tru1I TTAA 5 cut(s) 293, 366, 842, 930, 1009
Tru9I TTAA 5 cut(s) 293, 366, 842, 930, 1009
TscAI CASTG 3 cut(s) 364, 568, 775
TseFI GTSAC 2 cut(s) 164, 284
TseI GCWGC 2 cut(s) 114, 994
Tsp45I GTSAC 2 cut(s) 164, 284
TspDTI ATGAA 5 cut(s) 109, 627, 664, 774, 974
TspRI CASTG 3 cut(s) 364, 568, 775
Van91I CCANNNNNTGG 1 cut(s) 786
Vha464I CTTAAG 1 cut(s) 292
VspI ATTAAT 1 cut(s) 930
XbaI TCTAGA 1 cut(s) 952
XceI RCATGY 1 cut(s) 444
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XmiI GTMKAC 1 cut(s) 599
XspI CTAG 5 cut(s) 104, 149, 408, 647, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.