Rh3BG223300

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0012638
Physical Location & Seq
Reverse (-)
20364924 .. 20368400
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG223300.1

Sequence Viewer

Length: 1008 bp
ATGCAAAGTCCAGCAGAGAAAAAACCAGGCGTCTACACCTACGTGAGTCAATGGCCAATCTACTCCTTGGCTTGGTCAGTTCGCCAAGACAAGCCTTCACGCCTCGCCATAGGAAGCTTCATCGAAGAGTACAGCAACAAGGTCGAACTAGTCCAATTCAACCCGTCCACCTCCGACTTCACCACTGATAATCGCCTCATTTTCGACCACCCTTATGCCCCCACAAACATCATGTTCTTCCCTTCCGGGGCGGCCACCAATCCAGACCTCATAGCCACCTCCGGTGACTACTTACGCATTTGGGAGATCCATGACGACCGAATTGAGCTCAGGTCCCTGCTCAATGGGAACATGAGCAGCGAGTTCAACTCGGCCATAACATCATTTGATTGGGCCGAGTTTGACACTCGACGTGTGGCAACATCAAGTGTCGACACAACCTGTACGATTTGGGACATTGAAAGGGAGGCCGTGGACACCCAACTCGTAGCTCATGACAAAGAAGTGTATGACATTTCTTGGGGTGGATTCAATGTATTTGCTTCTGCTTCTGGGGATGGCACTGTTCGGATATTTGACCTAAGAGACAAAGAGAGGTCGACCATAGTATACGAGAACCCTGCTCAAGATGGGTCATTGTTAAGGCTAGAATGGAACAAACAAGACCCAAGGTTCATAGCCACGGTTGGAATGGACAGCAACAGGGTGGTGATATTGGACATTAGGTTTCCCACAGCACCATTGATGGAGCTCAAAAAGCATGGAGCTAGTGTGAATGCCATTTCTTGGTCGCCACGCATGGGGCACCAGCTATGTTCTGTGAGTGATGATTCAAGGGCTATGATATGGGAGGTGGTGAGATCCGGATTTGGATCCGATGGGGCGGACATGGAGCCGGAGATGTGGTATGGAGTGACGGCTCAGATTAATCAAGTGCGGTGGTCACCTGTGGAGTTGGATTGGGTTGCCATTGCTTTCTTGAACAAGTTGCAGCTATTGAAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

335

Amino Acids

37.96

Weight (kDa)

4.78

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 34 - 283 3.6e-09 EIPR1 beta-propeller
Beta-prop_WDR3_1st PF25173 104 - 204 1.1e-09 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 118 - 287 6.5e-12 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 120 - 316 9.2e-21 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 123 - 202 4.4e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 125 - 265 5e-16 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 125 - 199 4.7e-10 WDR5 beta-propeller domain
WD40_Gbeta PF25391 134 - 203 1.6e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 136 - 199 5.1e-06 WDR55
Beta-prop_THOC3 PF25174 215 - 285 2.8e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 804
AccB7I CCANNNNNTGG 1 cut(s) 786
AccI GTMKAC 4 cut(s) 33, 432, 599, 609
AccIII TCCGGA 1 cut(s) 863
AciI CCGC 3 cut(s) 251, 884, 937
AclWI GGATC 4 cut(s) 301, 855, 867, 880
AcoI YGGCCR 3 cut(s) 53, 252, 372
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 2 cut(s) 131, 445
AfiI CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 7 cut(s) 160, 367, 461, 532, 834, 982, 1000
AhlI ACTAGT 1 cut(s) 148
AjiI CACGTC 1 cut(s) 413
AjnI CCWGG 1 cut(s) 25
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 7 cut(s) 117, 328, 491, 751, 767, 811, 994
AluI AGCT 7 cut(s) 117, 328, 491, 751, 767, 811, 994
Alw21I GWGCWC 2 cut(s) 330, 753
Alw26I GTCTC 1 cut(s) 579
AlwI GGATC 4 cut(s) 301, 855, 867, 880
Aor13HI TCCGGA 1 cut(s) 863
AoxI GGCC 5 cut(s) 53, 252, 372, 393, 468
ApeKI GCWGC 2 cut(s) 357, 991
AseI ATTAAT 1 cut(s) 927
AspS9I GGNCC 2 cut(s) 333, 393
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 5 cut(s) 172, 296, 721, 868, 936
AvaII GGWCC 1 cut(s) 333
BaeGI GKGCMC 1 cut(s) 807
BalI TGGCCA 1 cut(s) 55
BamHI GGATCC 1 cut(s) 872
BanI GGYRCC 1 cut(s) 804
BanII GRGCYC 2 cut(s) 330, 753
Bbv12I GWGCWC 2 cut(s) 330, 753
BbvI GCAGC 2 cut(s) 369, 1003
BccI CCATC 4 cut(s) 551, 623, 739, 872
BceAI ACGGC 2 cut(s) 455, 933
BcgI CGANNNNNNTGC 4 cut(s) 124, 158, 602, 636
BciT130I CCWGG 1 cut(s) 27
BcnI CCSGG 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 579
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 3 cut(s) 149, 647, 768
BisI GCNGC 3 cut(s) 252, 358, 992
BlsI GCNGC 3 cut(s) 253, 359, 993
Bme1390I CCNGG 2 cut(s) 27, 247
Bme18I GGWCC 1 cut(s) 333
BmgBI CACGTC 1 cut(s) 413
BmgT120I GGNCC 2 cut(s) 333, 393
BmiI GGNNCC 4 cut(s) 335, 806, 874, 894
BmrFI CCNGG 2 cut(s) 27, 247
BplI GAGNNNNNCTC 2 cut(s) 353, 385
Bpu10I CCTNAGC 1 cut(s) 329
BpuEI CTTGAG 1 cut(s) 609
BpuMI CCSGG 1 cut(s) 247
BsaAI YACGTR 1 cut(s) 43
BsaBI GATNNNNATC 1 cut(s) 871
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 5 cut(s) 66, 246, 471, 668, 681
BsaWI WCCGGW 2 cut(s) 281, 863
BsaXI ACNNNNNCTCC 4 cut(s) 756, 786, 890, 920
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
Bse3DI GCAATG 1 cut(s) 969
Bse8I GATNNNNATC 1 cut(s) 871
BseAI TCCGGA 1 cut(s) 863
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 5 cut(s) 66, 246, 471, 668, 681
BseGI GGATG 1 cut(s) 562
BseJI GATNNNNATC 1 cut(s) 871
BseLI CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
BseMI GCAATG 1 cut(s) 969
BseMII CTCAG 2 cut(s) 343, 935
BseSI GKGCMC 1 cut(s) 807
BseXI GCAGC 2 cut(s) 369, 1003
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 5 cut(s) 55, 254, 374, 395, 470
BshNI GGYRCC 1 cut(s) 804
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 2 cut(s) 330, 753
BsiSI CCGG 4 cut(s) 246, 282, 864, 896
BslFI GGGAC 2 cut(s) 319, 467
BslI CCNNNNNNNGG 4 cut(s) 72, 247, 786, 800
BsmAI GTCTC 1 cut(s) 579
BsmFI GGGAC 2 cut(s) 319, 467
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 5 cut(s) 55, 254, 374, 395, 470
Bsp1286I GDGCHC 3 cut(s) 330, 753, 807
Bsp13I TCCGGA 1 cut(s) 863
Bsp143I GATC 3 cut(s) 306, 860, 872
BspACI CCGC 3 cut(s) 251, 884, 937
BspANI GGCC 5 cut(s) 55, 254, 374, 395, 470
BspCNI CTCAG 2 cut(s) 342, 934
BspEI TCCGGA 1 cut(s) 863
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 4 cut(s) 335, 806, 874, 894
BspPI GGATC 4 cut(s) 301, 855, 867, 880
BspT107I GGYRCC 1 cut(s) 804
BsrDI GCAATG 1 cut(s) 969
BssECI CCNNGG 5 cut(s) 66, 246, 471, 668, 681
BssMI GATC 3 cut(s) 306, 860, 872
BssNAI GTATAC 1 cut(s) 610
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 2 cut(s) 66, 668
Bst1107I GTATAC 1 cut(s) 610
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 565, 685
Bst6I CTCTTC 1 cut(s) 120
BstACI GRCGYC 1 cut(s) 30
BstBAI YACGTR 1 cut(s) 43
BstDEI CTNAG 3 cut(s) 329, 581, 921
BstDSI CCRYGG 2 cut(s) 471, 681
BstEII GGTNACC 1 cut(s) 942
BstF5I GGATG 1 cut(s) 562
BstKTI GATC 3 cut(s) 309, 863, 875
BstMAI GTCTC 1 cut(s) 579
BstMBI GATC 3 cut(s) 306, 860, 872
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 1 cut(s) 757
BstNI CCWGG 1 cut(s) 27
BstPI GGTNACC 1 cut(s) 942
BstSCI CCNGG 2 cut(s) 25, 245
BstSLI GKGCMC 1 cut(s) 807
BstV1I GCAGC 2 cut(s) 369, 1003
BstX2I RGATCY 3 cut(s) 306, 860, 872
BstYI RGATCY 3 cut(s) 306, 860, 872
BstZ17I GTATAC 1 cut(s) 610
BsuRI GGCC 5 cut(s) 55, 254, 374, 395, 470
BtgI CCRYGG 2 cut(s) 471, 681
BtrI CACGTC 1 cut(s) 413
BtsCI GGATG 1 cut(s) 562
BtsIMutI CAGTG 2 cut(s) 183, 561
CciI TCATGA 1 cut(s) 493
Cfr13I GGNCC 2 cut(s) 333, 393
CseI GACGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 130, 444
CspCI CAANNNNNGTGG 2 cut(s) 920, 955
CviAII CATG 7 cut(s) 232, 311, 352, 494, 761, 799, 889
CviQI GTAC 2 cut(s) 130, 444
DdeI CTNAG 3 cut(s) 329, 581, 921
DpnI GATC 3 cut(s) 308, 862, 874
DpnII GATC 3 cut(s) 306, 860, 872
EaeI YGGCCR 3 cut(s) 53, 252, 372
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
EciI GGCGGA 1 cut(s) 899
Ecl136II GAGCTC 2 cut(s) 328, 751
Eco130I CCWWGG 2 cut(s) 66, 668
Eco24I GRGCYC 2 cut(s) 330, 753
Eco47I GGWCC 1 cut(s) 333
Eco53kI GAGCTC 2 cut(s) 328, 751
Eco91I GGTNACC 1 cut(s) 942
EcoICRI GAGCTC 2 cut(s) 328, 751
EcoO109I RGGNCCY 1 cut(s) 333
EcoO65I GGTNACC 1 cut(s) 942
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 2 cut(s) 66, 668
EcoT38I GRGCYC 2 cut(s) 330, 753
ErhI CCWWGG 2 cut(s) 66, 668
FaeI CATG 7 cut(s) 235, 314, 355, 497, 764, 802, 892
FaqI GGGAC 2 cut(s) 319, 467
FatI CATG 7 cut(s) 231, 310, 351, 493, 760, 798, 888
FblI GTMKAC 4 cut(s) 33, 432, 599, 609
Fnu4HI GCNGC 3 cut(s) 252, 358, 992
FokI GGATG 1 cut(s) 569
FriOI GRGCYC 2 cut(s) 330, 753
Fsp4HI GCNGC 3 cut(s) 252, 358, 992
FspBI CTAG 3 cut(s) 149, 647, 768
GluI GCNGC 3 cut(s) 252, 358, 992
HaeIII GGCC 5 cut(s) 55, 254, 374, 395, 470
HapII CCGG 4 cut(s) 246, 282, 864, 896
HgaI GACGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 7 cut(s) 235, 314, 355, 497, 764, 802, 892
HincII GTYRAC 2 cut(s) 433, 600
HindII GTYRAC 2 cut(s) 433, 600
HindIII AAGCTT 1 cut(s) 115
HinfI GANTC 3 cut(s) 46, 528, 830
HpaII CCGG 4 cut(s) 246, 282, 864, 896
HphI GGTGA 5 cut(s) 172, 296, 721, 868, 936
Hpy166II GTNNAC 6 cut(s) 34, 168, 433, 475, 600, 610
Hpy188I TCNGA 4 cut(s) 175, 570, 877, 924
Hpy188III TCNNGA 5 cut(s) 263, 494, 626, 864, 979
Hpy8I GTNNAC 6 cut(s) 34, 168, 433, 475, 600, 610
Hpy99I CGWCG 1 cut(s) 414
HpyAV CCTTC 3 cut(s) 105, 252, 994
HpyCH4III ACNGT 2 cut(s) 565, 685
HpyCH4IV ACGT 2 cut(s) 42, 412
HpyCH4V TGCA 2 cut(s) 4, 991
HpyF10VI GCNNNNNNNGC 1 cut(s) 757
HpyF3I CTNAG 3 cut(s) 329, 581, 921
HpySE526I ACGT 2 cut(s) 42, 412
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 7 cut(s) 235, 314, 355, 497, 764, 802, 892
Kpn2I TCCGGA 1 cut(s) 863
Kzo9I GATC 3 cut(s) 306, 860, 872
LmnI GCTCC 3 cut(s) 748, 764, 892
Lsp1109I GCAGC 2 cut(s) 369, 1003
MaeI CTAG 3 cut(s) 149, 647, 768
MaeII ACGT 2 cut(s) 42, 412
MaeIII GTNAC 3 cut(s) 284, 913, 942
MalI GATC 3 cut(s) 308, 862, 874
MboI GATC 3 cut(s) 306, 860, 872
MboII GAAGA 2 cut(s) 137, 229
MflI RGATCY 3 cut(s) 306, 860, 872
MhlI GDGCHC 3 cut(s) 330, 753, 807
MlsI TGGCCA 1 cut(s) 55
MluCI AATT 2 cut(s) 155, 321
MluNI TGGCCA 1 cut(s) 55
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 3 cut(s) 198, 667, 936
MnlI CCTC 8 cut(s) 113, 181, 206, 278, 289, 460, 588, 844
Mox20I TGGCCA 1 cut(s) 55
MroI TCCGGA 1 cut(s) 863
MscI TGGCCA 1 cut(s) 55
MseI TTAA 2 cut(s) 641, 927
MslI CAYNNNNRTG 2 cut(s) 41, 213
Msp20I TGGCCA 1 cut(s) 55
MspI CCGG 4 cut(s) 246, 282, 864, 896
MspR9I CCNGG 2 cut(s) 27, 247
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 757
NciI CCSGG 1 cut(s) 247
NdeII GATC 3 cut(s) 306, 860, 872
NlaIII CATG 7 cut(s) 235, 314, 355, 497, 764, 802, 892
NlaIV GGNNCC 4 cut(s) 335, 806, 874, 894
NmeAIII GCCGAG 2 cut(s) 350, 421
NmuCI GTSAC 3 cut(s) 284, 913, 942
OliI CACNNNNGTG 1 cut(s) 41
PagI TCATGA 1 cut(s) 493
PctI GAATGC 1 cut(s) 781
PfeI GAWTC 2 cut(s) 528, 830
PflMI CCANNNNNTGG 1 cut(s) 786
PkrI GCNGC 3 cut(s) 253, 359, 993
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 43
PpuMI RGGWCCY 1 cut(s) 333
PshBI ATTAAT 1 cut(s) 927
Psp124BI GAGCTC 2 cut(s) 330, 753
Psp5II RGGWCCY 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 25
PspEI GGTNACC 1 cut(s) 942
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 4 cut(s) 335, 806, 874, 894
PspPI GGNCC 2 cut(s) 333, 393
PspPPI RGGWCCY 1 cut(s) 333
PsuI RGATCY 3 cut(s) 306, 860, 872
RsaI GTAC 2 cut(s) 131, 445
RsaNI GTAC 2 cut(s) 130, 444
RseI CAYNNNNRTG 2 cut(s) 41, 213
SacI GAGCTC 2 cut(s) 330, 753
SalI GTCGAC 2 cut(s) 431, 598
SaqAI TTAA 2 cut(s) 641, 927
SatI GCNGC 3 cut(s) 252, 358, 992
Sau3AI GATC 3 cut(s) 306, 860, 872
Sau96I GGNCC 2 cut(s) 333, 393
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 2 cut(s) 27, 247
SduI GDGCHC 3 cut(s) 330, 753, 807
SinI GGWCC 1 cut(s) 333
SmiMI CAYNNNNRTG 2 cut(s) 41, 213
SmlI CTYRAG 1 cut(s) 624
SmoI CTYRAG 1 cut(s) 624
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 2 cut(s) 155, 321
SsiI CCGC 3 cut(s) 251, 884, 937
SspMI CTAG 3 cut(s) 149, 647, 768
SstI GAGCTC 2 cut(s) 330, 753
StyD4I CCNGG 2 cut(s) 25, 245
StyI CCWWGG 2 cut(s) 66, 668
TaaI ACNGT 2 cut(s) 565, 685
TaiI ACGT 2 cut(s) 45, 415
TaqI TCGA 6 cut(s) 123, 144, 204, 409, 432, 599
TaqII GACCGA 1 cut(s) 333
TasI AATT 2 cut(s) 155, 321
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 528, 830
Tru1I TTAA 2 cut(s) 641, 927
Tru9I TTAA 2 cut(s) 641, 927
TscAI CASTG 2 cut(s) 190, 568
TseFI GTSAC 3 cut(s) 284, 913, 942
TseI GCWGC 2 cut(s) 357, 991
Tsp45I GTSAC 3 cut(s) 284, 913, 942
TspDTI ATGAA 2 cut(s) 109, 664
TspRI CASTG 2 cut(s) 190, 568
Van91I CCANNNNNTGG 1 cut(s) 786
VpaK11BI GGWCC 1 cut(s) 333
VspI ATTAAT 1 cut(s) 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XmiI GTMKAC 4 cut(s) 33, 432, 599, 609
XspI CTAG 3 cut(s) 149, 647, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.