FvH4_6g23820

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013576
Physical Location & Seq
Forward (+)
17904321 .. 17909711
5391 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g23820.t1

Sequence Viewer

Length: 1368 bp
ATGGCGTGCAGTGCCTCAACTTGCCAGTCAAATTGCTACAAAGACCAACCAGATGAACATGACCCAGATACCAATTCAGTTGCCAACAGAAACATCAGCAATGGTCTCTGCCTCAAGTGCAAGACCAACCCTCCCATCTCCTCCTCCGCCGACGACTCCCGCTTCTGCTCCGAATGCTTCCGCGCCAACCTCTTCGGCAAGTTCCGCTTCGCTGTCACCTCCAACGCCATGATTTCTCCCTCCGATAACGTCCTCCTCGCCTTCTCCGGCGGCCCTTCTTCCAGGGTTGCTCTAGAGTTTGTACACGAGATGCACAGCAAAGCGCAGAAGAATTTTGATGCCAGTAGGGACAGGTCATTACCGGTTTTCGGAGTTGGAGTTGCTTTCATTGATGAGGCTAGTGTTTATTCTTCTCCATCCAATGAACTATACAAAGCAATTCAAGAGATTACAGCTATTGTGGAGGAGCTAGCCCCGCCCGCGAAACAGTTGTATGTTGTGCCCATTGAGAGTGTTTACTCTTCAGAGTGTGGTGATGGAAGGGAGAGGCTGAAGAGGTTACTAGATGCTGTTACTGACCCCACTGGAAAAGAAGATCTTTTACTGCATCTCCGGATGTTGGCCTTGCAGAAGATTGCCTCGGAGAATGGATACAACAGAATTCTACTGGGATCATGCATATCGAGGATTGCTTGCCATGTTATCACAGCCACTGTGAAGGGACAAGGATATTCGCTACCAGCAGATATACAGTATGTAGATTCAAGGTGGGAAATCCCAGTATTGCTTCCTCTTCGTGATTGTCTTGCACAAGAGCTGAACATGCTTTGCCGCCTTGAGGGTCTAAAAACTGTTGAGTTGATAAGAAGTCCTGGCACCGGCATTAACGGGTTGGTTTTGTCATTCGTGTCACTCTTGCAGGAAGAAAATCCTTCTCGAGAGAGCACAATTGTGAGAACAGCTGGAAAGCTTATCCCATTTCATTTCAACAGAATTCAAGAGATCGATGATTCTAATGTCCCTTTGGCAACTCGAAGGCGCCAAAAGAGATATAATCTTAAACCGAATGAATGTTTTTCTTCAGAGTCATTCTGCTTTATCTGCAATGGCCCCCTCAGCAAATCTGATTTGCTAAGTTTGCGCAATCTTGGTCAATGCCAAACAAGTCCTGACACTTTTGGTGCTGCCTGCTGTTCGAGCTGCCGGTTTCAGATACTTCCCCAGGACCCTTCGTTACTGGATCAATTTTCTGCAGATTTACCTCAGCAGCTGGTTGCTCGAGCAAAGCTTGACAACCTTGACAATTACCGTATGCTCAGGGAACAAATACAAGATTGCTTGCTTTCAGAAGGCGAAGATGAAACCTGA

Protein Analysis

456

Amino Acids

50.37

Weight (kDa)

5.38

Isoelectric Point (pI)

56.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1142
AccB1I GGYRCC 2 cut(s) 875, 1038
AccII CGCG 2 cut(s) 183, 482
AccIII TCCGGA 1 cut(s) 612
AciI CCGC 8 cut(s) 147, 160, 181, 205, 270, 476, 480, 832
AclWI GGATC 2 cut(s) 679, 1248
AcsI RAATTY 3 cut(s) 331, 660, 993
AcuI CTGAAG 3 cut(s) 507, 572, 1065
AcyI GRCGYC 1 cut(s) 1039
AfaI GTAC 1 cut(s) 303
AfiI CCNNNNNNNGG 4 cut(s) 368, 619, 838, 878
AgeI ACCGGT 1 cut(s) 361
AgsI TTSAA 4 cut(s) 443, 765, 988, 998
AjnI CCWGG 3 cut(s) 281, 871, 1221
AleI CACNNNNGTG 1 cut(s) 950
AluBI AGCT 8 cut(s) 455, 469, 817, 962, 970, 1200, 1270, 1288
AluI AGCT 8 cut(s) 455, 469, 817, 962, 970, 1200, 1270, 1288
Alw21I GWGCWC 1 cut(s) 947
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 2 cut(s) 679, 1248
AlwNI CAGNNNCTG 2 cut(s) 713, 1270
Ama87I CYCGRG 2 cut(s) 936, 1278
Aor13HI TCCGGA 1 cut(s) 612
AoxI GGCC 3 cut(s) 271, 621, 1108
ApeKI GCWGC 3 cut(s) 1184, 1200, 1267
ApoI RAATTY 3 cut(s) 331, 660, 993
AsiGI ACCGGT 1 cut(s) 361
AspLEI GCGC 4 cut(s) 185, 325, 1041, 1143
AspS9I GGNCC 3 cut(s) 272, 1109, 1225
AsuHPI GGTGA 2 cut(s) 208, 545
AsuNHI GCTAGC 1 cut(s) 469
AvaI CYCGRG 2 cut(s) 936, 1278
AvaII GGWCC 1 cut(s) 1225
BaeGI GKGCMC 1 cut(s) 504
BanI GGYRCC 2 cut(s) 875, 1038
BarI GAAGNNNNNNTAC 2 cut(s) 585, 617
BauI CACGAG 1 cut(s) 305
Bbv12I GWGCWC 1 cut(s) 947
BbvCI CCTCAGC 2 cut(s) 1115, 1263
BbvI GCAGC 3 cut(s) 1171, 1187, 1279
BccI CCATC 3 cut(s) 143, 424, 530
BciT130I CCWGG 3 cut(s) 283, 873, 1223
BciVI GTATCC 1 cut(s) 644
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 4 cut(s) 293, 399, 470, 563
BfmI CTRYAG 1 cut(s) 1251
BfoI RGCGCY 1 cut(s) 1042
BfuI GTATCC 1 cut(s) 644
BglII AGATCT 1 cut(s) 595
BisI GCNGC 5 cut(s) 271, 832, 1185, 1201, 1268
BlsI GCNGC 5 cut(s) 272, 833, 1186, 1202, 1269
Bme1390I CCNGG 3 cut(s) 283, 873, 1223
Bme18I GGWCC 1 cut(s) 1225
BmeT110I CYCGRG 2 cut(s) 936, 1278
BmgT120I GGNCC 3 cut(s) 272, 1109, 1225
BmiI GGNNCC 4 cut(s) 877, 1040, 1111, 1227
BmrFI CCNGG 3 cut(s) 283, 873, 1223
BmrI ACTGGG 2 cut(s) 677, 773
BmsI GCATC 4 cut(s) 300, 328, 556, 616
BmtI GCTAGC 1 cut(s) 473
BmuI ACTGGG 2 cut(s) 677, 773
Bpu10I CCTNAGC 3 cut(s) 1115, 1263, 1316
BpuEI CTTGAG 2 cut(s) 98, 857
Bsa29I ATCGAT 1 cut(s) 1005
BsaHI GRCGYC 1 cut(s) 1039
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 3 cut(s) 282, 639, 1221
BsaWI WCCGGW 2 cut(s) 361, 612
BsaXI ACNNNNNCTCC 6 cut(s) 115, 145, 363, 393, 594, 624
Bsc4I CCNNNNNNNGG 4 cut(s) 368, 619, 838, 878
Bse118I RCCGGY 3 cut(s) 361, 878, 1203
Bse1I ACTGG 6 cut(s) 25, 342, 589, 672, 779, 1242
Bse3DI GCAATG 2 cut(s) 106, 1111
BseAI TCCGGA 1 cut(s) 612
BseBI CCWGG 3 cut(s) 283, 873, 1223
BseCI ATCGAT 1 cut(s) 1005
BseDI CCNNGG 3 cut(s) 282, 639, 1221
BseGI GGATG 2 cut(s) 416, 621
BseLI CCNNNNNNNGG 4 cut(s) 368, 619, 838, 878
BseMI GCAATG 2 cut(s) 106, 1111
BseMII CTCAG 3 cut(s) 1129, 1277, 1330
BseNI ACTGG 6 cut(s) 25, 342, 589, 672, 779, 1242
BseRI GAGGAG 4 cut(s) 130, 133, 245, 479
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 3 cut(s) 1171, 1187, 1279
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 2 cut(s) 183, 482
BshFI GGCC 3 cut(s) 273, 623, 1110
BshNI GGYRCC 2 cut(s) 875, 1038
BshTI ACCGGT 1 cut(s) 361
BshVI ATCGAT 1 cut(s) 1005
BsiHKAI GWGCWC 1 cut(s) 947
BsiHKCI CYCGRG 2 cut(s) 936, 1278
BsiSI CCGG 5 cut(s) 267, 362, 613, 879, 1204
BslFI GGGAC 3 cut(s) 362, 735, 1004
BslI CCNNNNNNNGG 4 cut(s) 368, 619, 838, 878
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 3 cut(s) 362, 735, 1004
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 3 cut(s) 273, 623, 1110
Bso31I GGTCTC 1 cut(s) 110
BsoBI CYCGRG 2 cut(s) 936, 1278
Bsp1286I GDGCHC 2 cut(s) 504, 947
Bsp13I TCCGGA 1 cut(s) 612
Bsp1407I TGTACA 1 cut(s) 301
Bsp143I GATC 4 cut(s) 595, 671, 1002, 1240
BspACI CCGC 8 cut(s) 147, 160, 181, 205, 270, 476, 480, 832
BspANI GGCC 3 cut(s) 273, 623, 1110
BspCNI CTCAG 3 cut(s) 1128, 1276, 1329
BspDI ATCGAT 1 cut(s) 1005
BspEI TCCGGA 1 cut(s) 612
BspFNI CGCG 2 cut(s) 183, 482
BspLI GGNNCC 4 cut(s) 877, 1040, 1111, 1227
BspMAI CTGCAG 1 cut(s) 1255
BspOI GCTAGC 1 cut(s) 473
BspPI GGATC 2 cut(s) 679, 1248
BspT107I GGYRCC 2 cut(s) 875, 1038
BspTNI GGTCTC 1 cut(s) 110
BsrDI GCAATG 2 cut(s) 106, 1111
BsrFI RCCGGY 3 cut(s) 361, 878, 1203
BsrGI TGTACA 1 cut(s) 301
BsrI ACTGG 6 cut(s) 25, 342, 589, 672, 779, 1242
BssAI RCCGGY 3 cut(s) 361, 878, 1203
BssECI CCNNGG 3 cut(s) 282, 639, 1221
BssMI GATC 4 cut(s) 595, 671, 1002, 1240
BssNI GRCGYC 1 cut(s) 1039
BssSI CACGAG 1 cut(s) 305
Bst2BI CACGAG 1 cut(s) 305
Bst2UI CCWGG 3 cut(s) 283, 873, 1223
Bst4CI ACNGT 5 cut(s) 489, 715, 753, 853, 1310
Bst6I CTCTTC 4 cut(s) 197, 526, 548, 798
BstACI GRCGYC 1 cut(s) 1039
BstAUI TGTACA 1 cut(s) 301
BstC8I GCNNGC 6 cut(s) 7, 471, 480, 694, 1189, 1340
BstDEI CTNAG 4 cut(s) 1115, 1133, 1263, 1316
BstF5I GGATG 2 cut(s) 416, 621
BstFNI CGCG 2 cut(s) 183, 482
BstH2I RGCGCY 1 cut(s) 1042
BstHHI GCGC 4 cut(s) 185, 325, 1041, 1143
BstKTI GATC 4 cut(s) 598, 674, 1005, 1243
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 4 cut(s) 595, 671, 1002, 1240
BstNI CCWGG 3 cut(s) 283, 873, 1223
BstNSI RCATGY 1 cut(s) 826
BstSCI CCNGG 3 cut(s) 281, 871, 1221
BstSFI CTRYAG 1 cut(s) 1251
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 2 cut(s) 183, 482
BstV1I GCAGC 3 cut(s) 1171, 1187, 1279
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
Bsu15I ATCGAT 1 cut(s) 1005
BsuI GTATCC 1 cut(s) 644
BsuRI GGCC 3 cut(s) 273, 623, 1110
BsuTUI ATCGAT 1 cut(s) 1005
BtsCI GGATG 2 cut(s) 416, 621
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 3 cut(s) 16, 582, 711
Cac8I GCNNGC 6 cut(s) 7, 471, 480, 694, 1189, 1340
CaiI CAGNNNCTG 2 cut(s) 713, 1270
CfoI GCGC 4 cut(s) 185, 325, 1041, 1143
Cfr10I RCCGGY 3 cut(s) 361, 878, 1203
Cfr13I GGNCC 3 cut(s) 272, 1109, 1225
ClaI ATCGAT 1 cut(s) 1005
Csp6I GTAC 1 cut(s) 302
CspAI ACCGGT 1 cut(s) 361
CviAII CATG 5 cut(s) 59, 229, 675, 698, 823
CviQI GTAC 1 cut(s) 302
DdeI CTNAG 4 cut(s) 1115, 1133, 1263, 1316
DinI GGCGCC 1 cut(s) 1040
DpnI GATC 4 cut(s) 597, 673, 1004, 1242
DpnII GATC 4 cut(s) 595, 671, 1002, 1240
Eam1104I CTCTTC 4 cut(s) 197, 526, 548, 798
EarI CTCTTC 4 cut(s) 197, 526, 548, 798
EciI GGCGGA 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 1225
Eco57I CTGAAG 3 cut(s) 507, 572, 1065
Eco88I CYCGRG 2 cut(s) 936, 1278
EcoO109I RGGNCCY 1 cut(s) 1225
EcoRI GAATTC 2 cut(s) 660, 993
EcoRII CCWGG 3 cut(s) 281, 871, 1221
EcoT22I ATGCAT 1 cut(s) 680
EgeI GGCGCC 1 cut(s) 1040
EheI GGCGCC 1 cut(s) 1040
FaeI CATG 5 cut(s) 62, 232, 678, 701, 826
FalI AAGNNNNNCTT 4 cut(s) 191, 223, 582, 614
FaqI GGGAC 3 cut(s) 362, 735, 1004
FatI CATG 5 cut(s) 58, 228, 674, 697, 822
FauI CCCGC 3 cut(s) 167, 483, 487
Fnu4HI GCNGC 5 cut(s) 271, 832, 1185, 1201, 1268
FokI GGATG 2 cut(s) 403, 628
Fsp4HI GCNGC 5 cut(s) 271, 832, 1185, 1201, 1268
FspBI CTAG 4 cut(s) 293, 399, 470, 563
FspI TGCGCA 1 cut(s) 1142
GlaI GCGC 4 cut(s) 184, 324, 1040, 1142
GluI GCNGC 5 cut(s) 271, 832, 1185, 1201, 1268
HaeII RGCGCY 1 cut(s) 1042
HaeIII GGCC 3 cut(s) 273, 623, 1110
HapII CCGG 5 cut(s) 267, 362, 613, 879, 1204
HhaI GCGC 4 cut(s) 185, 325, 1041, 1143
Hin1I GRCGYC 1 cut(s) 1039
Hin1II CATG 5 cut(s) 62, 232, 678, 701, 826
Hin6I GCGC 4 cut(s) 183, 323, 1039, 1141
HinP1I GCGC 4 cut(s) 183, 323, 1039, 1141
HindIII AAGCTT 2 cut(s) 968, 1286
HinfI GANTC 4 cut(s) 155, 761, 1010, 1085
HpaII CCGG 5 cut(s) 267, 362, 613, 879, 1204
HphI GGTGA 2 cut(s) 208, 545
Hpy166II GTNNAC 2 cut(s) 304, 517
Hpy188I TCNGA 9 cut(s) 172, 244, 371, 526, 643, 1084, 1126, 1212, 1348
Hpy188III TCNNGA 8 cut(s) 293, 443, 613, 797, 936, 938, 998, 1169
Hpy8I GTNNAC 2 cut(s) 304, 517
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 8 cut(s) 271, 285, 534, 712, 942, 1029, 1239, 1343
HpyCH4III ACNGT 5 cut(s) 489, 715, 753, 853, 1310
HpyCH4IV ACGT 1 cut(s) 249
HpyF3I CTNAG 4 cut(s) 1115, 1133, 1263, 1316
HpySE526I ACGT 1 cut(s) 249
Hsp92I GRCGYC 1 cut(s) 1039
Hsp92II CATG 5 cut(s) 62, 232, 678, 701, 826
HspAI GCGC 4 cut(s) 183, 323, 1039, 1141
KasI GGCGCC 1 cut(s) 1038
Kpn2I TCCGGA 1 cut(s) 612
Kzo9I GATC 4 cut(s) 595, 671, 1002, 1240
LmnI GCTCC 2 cut(s) 173, 466
Lsp1109I GCAGC 3 cut(s) 1171, 1187, 1279
LweI GCATC 4 cut(s) 300, 328, 556, 616
MaeI CTAG 4 cut(s) 293, 399, 470, 563
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 5 cut(s) 214, 558, 571, 909, 1233
MalI GATC 4 cut(s) 597, 673, 1004, 1242
MboI GATC 4 cut(s) 595, 671, 1002, 1240
MfeI CAATTG 1 cut(s) 948
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 2 cut(s) 504, 947
MluCI AATT 9 cut(s) 31, 73, 331, 438, 660, 948, 993, 1244, 1303
Mly113I GGCGCC 1 cut(s) 1039
MlyI GAGTC 2 cut(s) 149, 1094
MmeI TCCRAC 2 cut(s) 246, 355
Mph1103I ATGCAT 1 cut(s) 680
MroI TCCGGA 1 cut(s) 612
MseI TTAA 2 cut(s) 885, 1059
MslI CAYNNNNRTG 1 cut(s) 950
MspA1I CMGCKG 2 cut(s) 962, 1270
MspI CCGG 5 cut(s) 267, 362, 613, 879, 1204
MspR9I CCNGG 3 cut(s) 283, 873, 1223
MunI CAATTG 1 cut(s) 948
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 3 cut(s) 283, 873, 1223
MvnI CGCG 2 cut(s) 183, 482
NarI GGCGCC 1 cut(s) 1039
NdeII GATC 4 cut(s) 595, 671, 1002, 1240
NheI GCTAGC 1 cut(s) 469
NlaIII CATG 5 cut(s) 62, 232, 678, 701, 826
NlaIV GGNNCC 4 cut(s) 877, 1040, 1111, 1227
NmuCI GTSAC 2 cut(s) 214, 909
NsbI TGCGCA 1 cut(s) 1142
NsiI ATGCAT 1 cut(s) 680
NspI RCATGY 1 cut(s) 826
OliI CACNNNNGTG 1 cut(s) 950
PaeR7I CTCGAG 2 cut(s) 936, 1278
PctI GAATGC 1 cut(s) 179
PfeI GAWTC 2 cut(s) 761, 1010
PinAI ACCGGT 1 cut(s) 361
PkrI GCNGC 5 cut(s) 272, 833, 1186, 1202, 1269
PleI GAGTC 2 cut(s) 149, 1093
PluTI GGCGCC 1 cut(s) 1042
PpsI GAGTC 2 cut(s) 149, 1093
PpuMI RGGWCCY 1 cut(s) 1225
Psp5II RGGWCCY 1 cut(s) 1225
Psp6I CCWGG 3 cut(s) 281, 871, 1221
PspGI CCWGG 3 cut(s) 281, 871, 1221
PspN4I GGNNCC 4 cut(s) 877, 1040, 1111, 1227
PspPI GGNCC 3 cut(s) 272, 1109, 1225
PspPPI RGGWCCY 1 cut(s) 1225
PspXI VCTCGAGB 1 cut(s) 1278
PstI CTGCAG 1 cut(s) 1255
PstNI CAGNNNCTG 2 cut(s) 713, 1270
PsuI RGATCY 1 cut(s) 595
PvuII CAGCTG 2 cut(s) 962, 1270
RsaI GTAC 1 cut(s) 303
RsaNI GTAC 1 cut(s) 302
RseI CAYNNNNRTG 1 cut(s) 950
SaqAI TTAA 2 cut(s) 885, 1059
SatI GCNGC 5 cut(s) 271, 832, 1185, 1201, 1268
Sau3AI GATC 4 cut(s) 595, 671, 1002, 1240
Sau96I GGNCC 3 cut(s) 272, 1109, 1225
SchI GAGTC 2 cut(s) 149, 1094
ScrFI CCNGG 3 cut(s) 283, 873, 1223
SduI GDGCHC 2 cut(s) 504, 947
SfaNI GCATC 4 cut(s) 300, 328, 556, 616
SfcI CTRYAG 1 cut(s) 1251
SfoI GGCGCC 1 cut(s) 1040
Sfr274I CTCGAG 2 cut(s) 936, 1278
SinI GGWCC 1 cut(s) 1225
SlaI CTCGAG 2 cut(s) 936, 1278
SmiMI CAYNNNNRTG 1 cut(s) 950
SmlI CTYRAG 4 cut(s) 113, 836, 936, 1278
SmoI CTYRAG 4 cut(s) 113, 836, 936, 1278
Sse9I AATT 9 cut(s) 31, 73, 331, 438, 660, 948, 993, 1244, 1303
SsiI CCGC 8 cut(s) 147, 160, 181, 205, 270, 476, 480, 832
SspDI GGCGCC 1 cut(s) 1038
SspMI CTAG 4 cut(s) 293, 399, 470, 563
StyD4I CCNGG 3 cut(s) 281, 871, 1221
TaaI ACNGT 5 cut(s) 489, 715, 753, 853, 1310
TaiI ACGT 1 cut(s) 252
TaqI TCGA 6 cut(s) 683, 937, 1005, 1033, 1196, 1279
TasI AATT 9 cut(s) 31, 73, 331, 438, 660, 948, 993, 1244, 1303
TatI WGTACW 1 cut(s) 301
TauI GCSGC 2 cut(s) 273, 834
TfiI GAWTC 2 cut(s) 761, 1010
Tru1I TTAA 2 cut(s) 885, 1059
Tru9I TTAA 2 cut(s) 885, 1059
TscAI CASTG 3 cut(s) 16, 589, 718
TseFI GTSAC 2 cut(s) 214, 909
TseI GCWGC 3 cut(s) 1184, 1200, 1267
Tsp45I GTSAC 2 cut(s) 214, 909
TspDTI ATGAA 5 cut(s) 69, 376, 438, 971, 1083
TspRI CASTG 3 cut(s) 16, 589, 718
VpaK11BI GGWCC 1 cut(s) 1225
XapI RAATTY 3 cut(s) 331, 660, 993
XbaI TCTAGA 1 cut(s) 292
XceI RCATGY 1 cut(s) 826
XhoI CTCGAG 2 cut(s) 936, 1278
XspI CTAG 4 cut(s) 293, 399, 470, 563
Zsp2I ATGCAT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.