Prupe.7G060900_v2.0.a1

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0013576
Physical Location & Seq
Reverse (-)
10019125 .. 10023816
4692 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G060900.5

Sequence Viewer

Length: 1392 bp
ATGGCGTGCAGTGCTTCAGCTTGCCAGTCAAATTGCTACAAAACCCAGCAGGAAGAAGAAGCTGTTAAAGCCAACAACAATACCAATTCATTTGCTGCTAATATTAGCAGCAGCAACAACAACAGCAACAGTGGTTTGTGTCTGAAGTGCAAGGCCAACCAACCTATCTCTTCCAATAGCGACGGTGGTGACGATGCACGCTTTTGCATCGATTGCTTCCGCGGCAATCTCTTCGGGAAGTTCAGGTTCGCTGTGACCTCCAACGCCATGATTGCTCCTACCGATAATGTCCTCGTTGCCTTCTCTGGTGGCCCTTCTTCCAGGGTTGCCTTGCAGTTTGTACATGAGATGCATAGTAAAGCGCAGAAGAATTTCGATGCATGTAGGGATAGATCGTTGCCAGTTTTCGGTGTTGGAGTTGCATTTGTTGATGAAAGTTCTGTTTACTCTGCCCCCTCTAATGAAACCGACAAAGTAATTGGAGACGTTAGATTGATAGTGGAAAATCTAGCCCCGCCATCAAAGGAGTTGTATATTGTACCTATTGAAAGTGTCTACTCTTCAGATTCTGGTGATGGAAGGGAGAGATTAAAGAAGTTACTGGATGCTGTTGGTGATGCAACTGGGAGAGAAGATCTCTTATTACATCTACGCATGTTGGCCTTGCAAAAGGTTGCCTCTCAAAATGGATACAACAGACTTCTACTGGGGTCATGCGTATCGAGGATTGCCTGCCATGTTATTTCAGCCACTGTCAAGGGCCAAGGATATTCGTTACCAGCCGATGTACAGTATGTTGATGCAAGGTGGGAGATCCCAGTTGTGCTTCCCCTTCGCGATTGTCTTGCCAAAGAGCTGACCATGCTTTGCCGCCTTGATGGTCTAAAAACTGTGGAGTTGATAAGAAGTCCTTGCACTGGCATAAATGGCTTGGTCTCATCATTTGTGACACTTTTGCAGGAAGAAAATCCTTCTCGAGAGTGCACAATTATGAGAACAGCTGGAAAGCTTATCCCATTTCATTTCAACAAAATTGAAGAGATTGATGACTCTAATGTTCTTTTGGCAACTCGAAGGCGTCAGAAGAGATATAACCTTAAACCTAATGAATCTTTTTCCTCCGAGTCGTTCTGCTTTATCTGCAATAGCCCACTCAGCAGATCTGACTTGCTAAGTTTGAAGAACCTGGAGAGTCACAAAACAAGTTCTGTTTGCTGTTCAAGCTGCCAGTTCCAAATACTTCCCCAAGACCCCTCATCAGTAAATGAGTTGTACACTCTTTTACCTGAGCAACTGTTTGCCCGAGCAAAGCATGACAACCTTGACCATTGCAGTGCCATCAGGGAACAAATACAAGATTGCTTGCTTTCAGAAGGTGAAGATGAAACTTGA

Protein Analysis

464

Amino Acids

50.88

Weight (kDa)

5.73

Isoelectric Point (pI)

55.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 555
AccII CGCG 2 cut(s) 222, 837
AciI CCGC 4 cut(s) 220, 222, 515, 871
AclWI GGATC 1 cut(s) 808
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 2 cut(s) 164, 546
AcyI GRCGYC 1 cut(s) 1078
AfaI GTAC 4 cut(s) 342, 540, 789, 1274
AfiI CCNNNNNNNGG 2 cut(s) 407, 917
AgsI TTSAA 5 cut(s) 548, 1027, 1037, 1180, 1221
AjnI CCWGG 2 cut(s) 320, 1185
AluBI AGCT 6 cut(s) 20, 62, 856, 1001, 1009, 1224
AluI AGCT 6 cut(s) 20, 62, 856, 1001, 1009, 1224
Alw21I GWGCWC 1 cut(s) 986
Alw26I GTCTC 2 cut(s) 477, 940
Alw44I GTGCAC 1 cut(s) 982
AlwI GGATC 1 cut(s) 808
AlwNI CAGNNNCTG 2 cut(s) 569, 752
Ama87I CYCGRG 2 cut(s) 975, 1302
AoxI GGCC 4 cut(s) 153, 310, 660, 760
ApaLI GTGCAC 1 cut(s) 982
ApeKI GCWGC 4 cut(s) 95, 108, 111, 1224
ApoI RAATTY 1 cut(s) 370
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 2 cut(s) 311, 760
AsuHPI GGTGA 4 cut(s) 200, 584, 626, 1388
AvaI CYCGRG 2 cut(s) 975, 1302
BaeGI GKGCMC 1 cut(s) 986
Bbv12I GWGCWC 1 cut(s) 986
BbvI GCAGC 4 cut(s) 82, 120, 123, 1211
BccI CCATC 4 cut(s) 526, 569, 872, 1346
BcgI CGANNNNNNTGC 4 cut(s) 214, 248, 827, 861
BciT130I CCWGG 2 cut(s) 322, 1187
BciVI GTATCC 1 cut(s) 683
BcoDI GTCTC 2 cut(s) 477, 940
BfaI CTAG 1 cut(s) 509
BfuI GTATCC 1 cut(s) 683
BglII AGATCT 2 cut(s) 634, 1160
BisI GCNGC 6 cut(s) 96, 109, 112, 223, 871, 1225
BlsI GCNGC 6 cut(s) 97, 110, 113, 224, 872, 1226
Bme1390I CCNGG 2 cut(s) 322, 1187
BmeT110I CYCGRG 2 cut(s) 975, 1302
BmgT120I GGNCC 2 cut(s) 311, 760
BmrFI CCNGG 2 cut(s) 322, 1187
BmrI ACTGGG 3 cut(s) 633, 716, 812
BmsI GCATC 7 cut(s) 184, 216, 339, 367, 595, 607, 790
BmuI ACTGGG 3 cut(s) 633, 716, 812
BplI GAGNNNNNCTC 2 cut(s) 621, 653
BpmI CTGGAG 1 cut(s) 1208
Bpu10I CCTNAGC 1 cut(s) 1287
Bsa29I ATCGAT 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 1078
BsaI GGTCTC 1 cut(s) 940
BsaJI CCNNGG 3 cut(s) 220, 321, 763
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 917
Bse1I ACTGG 8 cut(s) 25, 401, 606, 628, 711, 818, 922, 1228
Bse3DI GCAATG 1 cut(s) 1327
BseBI CCWGG 2 cut(s) 322, 1187
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 3 cut(s) 220, 321, 763
BseGI GGATG 1 cut(s) 610
BseLI CCNNNNNNNGG 2 cut(s) 407, 917
BseMI GCAATG 1 cut(s) 1327
BseMII CTCAG 2 cut(s) 1168, 1278
BseNI ACTGG 8 cut(s) 25, 401, 606, 628, 711, 818, 922, 1228
BseSI GKGCMC 1 cut(s) 986
BseXI GCAGC 4 cut(s) 82, 120, 123, 1211
BseYI CCCAGC 1 cut(s) 45
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 2 cut(s) 222, 837
BshFI GGCC 4 cut(s) 155, 312, 662, 762
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 986
BsiHKCI CYCGRG 2 cut(s) 975, 1302
BslI CCNNNNNNNGG 2 cut(s) 407, 917
BsmAI GTCTC 2 cut(s) 477, 940
BsmBI CGTCTC 1 cut(s) 477
BsnI GGCC 4 cut(s) 155, 312, 662, 762
Bso31I GGTCTC 1 cut(s) 940
BsoBI CYCGRG 2 cut(s) 975, 1302
Bsp1286I GDGCHC 1 cut(s) 986
Bsp1407I TGTACA 3 cut(s) 340, 787, 1272
Bsp143I GATC 4 cut(s) 392, 634, 813, 1160
Bsp68I TCGCGA 1 cut(s) 837
BspACI CCGC 4 cut(s) 220, 222, 515, 871
BspANI GGCC 4 cut(s) 155, 312, 662, 762
BspCNI CTCAG 2 cut(s) 1167, 1279
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 2 cut(s) 222, 837
BspPI GGATC 1 cut(s) 808
BspTNI GGTCTC 1 cut(s) 940
BsrDI GCAATG 1 cut(s) 1327
BsrGI TGTACA 3 cut(s) 340, 787, 1272
BsrI ACTGG 8 cut(s) 25, 401, 606, 628, 711, 818, 922, 1228
BssECI CCNNGG 3 cut(s) 220, 321, 763
BssMI GATC 4 cut(s) 392, 634, 813, 1160
BssNI GRCGYC 1 cut(s) 1078
BssT1I CCWWGG 1 cut(s) 763
Bst2UI CCWGG 2 cut(s) 322, 1187
Bst4CI ACNGT 6 cut(s) 131, 185, 754, 792, 892, 1296
Bst6I CTCTTC 5 cut(s) 175, 236, 565, 1032, 1079
BstACI GRCGYC 1 cut(s) 1078
BstAPI GCANNNNNTGC 1 cut(s) 213
BstAUI TGTACA 3 cut(s) 340, 787, 1272
BstC8I GCNNGC 5 cut(s) 7, 22, 199, 733, 1364
BstDEI CTNAG 3 cut(s) 1154, 1172, 1287
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 1 cut(s) 610
BstFNI CGCG 2 cut(s) 222, 837
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 4 cut(s) 395, 637, 816, 1163
BstMAI GTCTC 2 cut(s) 477, 940
BstMBI GATC 4 cut(s) 392, 634, 813, 1160
BstNI CCWGG 2 cut(s) 322, 1187
BstNSI RCATGY 2 cut(s) 384, 658
BstSCI CCNGG 2 cut(s) 320, 1185
BstSLI GKGCMC 1 cut(s) 986
BstUI CGCG 2 cut(s) 222, 837
BstV1I GCAGC 4 cut(s) 82, 120, 123, 1211
BstX2I RGATCY 3 cut(s) 634, 813, 1160
BstYI RGATCY 3 cut(s) 634, 813, 1160
Bsu15I ATCGAT 1 cut(s) 210
BsuI GTATCC 1 cut(s) 683
BsuRI GGCC 4 cut(s) 155, 312, 662, 762
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 1 cut(s) 610
BtsI GCAGTG 2 cut(s) 16, 1339
BtsIMutI CAGTG 5 cut(s) 16, 136, 750, 915, 1339
BtuMI TCGCGA 1 cut(s) 837
Cac8I GCNNGC 5 cut(s) 7, 22, 199, 733, 1364
CaiI CAGNNNCTG 2 cut(s) 569, 752
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 2 cut(s) 311, 760
Cfr42I CCGCGG 1 cut(s) 223
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 1 cut(s) 1067
Csp6I GTAC 4 cut(s) 341, 539, 788, 1273
CviAII CATG 8 cut(s) 268, 344, 381, 655, 714, 737, 862, 1313
CviQI GTAC 4 cut(s) 341, 539, 788, 1273
DdeI CTNAG 3 cut(s) 1154, 1172, 1287
DpnI GATC 4 cut(s) 394, 636, 815, 1162
DpnII GATC 4 cut(s) 392, 634, 813, 1160
Eam1104I CTCTTC 5 cut(s) 175, 236, 565, 1032, 1079
EarI CTCTTC 5 cut(s) 175, 236, 565, 1032, 1079
Eco130I CCWWGG 1 cut(s) 763
Eco31I GGTCTC 1 cut(s) 940
Eco57I CTGAAG 2 cut(s) 164, 546
Eco88I CYCGRG 2 cut(s) 975, 1302
EcoRII CCWGG 2 cut(s) 320, 1185
EcoT14I CCWWGG 1 cut(s) 763
EcoT22I ATGCAT 2 cut(s) 354, 382
ErhI CCWWGG 1 cut(s) 763
Esp3I CGTCTC 1 cut(s) 477
FaeI CATG 8 cut(s) 271, 347, 384, 658, 717, 740, 865, 1316
FalI AAGNNNNNCTT 2 cut(s) 895, 927
FatI CATG 8 cut(s) 267, 343, 380, 654, 713, 736, 861, 1312
FauI CCCGC 1 cut(s) 522
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 6 cut(s) 96, 109, 112, 223, 871, 1225
FokI GGATG 1 cut(s) 617
Fsp4HI GCNGC 6 cut(s) 96, 109, 112, 223, 871, 1225
FspBI CTAG 1 cut(s) 509
GlaI GCGC 1 cut(s) 363
GluI GCNGC 6 cut(s) 96, 109, 112, 223, 871, 1225
GsaI CCCAGC 1 cut(s) 49
GsuI CTGGAG 1 cut(s) 1208
HaeIII GGCC 4 cut(s) 155, 312, 662, 762
HgaI GACGC 1 cut(s) 1067
HhaI GCGC 1 cut(s) 364
Hin1I GRCGYC 1 cut(s) 1078
Hin1II CATG 8 cut(s) 271, 347, 384, 658, 717, 740, 865, 1316
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HindIII AAGCTT 1 cut(s) 1007
HinfI GANTC 5 cut(s) 566, 1049, 1109, 1124, 1192
HphI GGTGA 4 cut(s) 200, 584, 626, 1388
Hpy166II GTNNAC 4 cut(s) 445, 556, 984, 1275
Hpy188I TCNGA 6 cut(s) 144, 565, 1083, 1123, 1165, 1372
Hpy188III TCNNGA 4 cut(s) 235, 836, 975, 977
Hpy8I GTNNAC 4 cut(s) 445, 556, 984, 1275
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 7 cut(s) 310, 324, 573, 842, 981, 1068, 1367
HpyCH4III ACNGT 6 cut(s) 131, 185, 754, 792, 892, 1296
HpyCH4IV ACGT 1 cut(s) 486
HpyF3I CTNAG 3 cut(s) 1154, 1172, 1287
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 1078
Hsp92II CATG 8 cut(s) 271, 347, 384, 658, 717, 740, 865, 1316
HspAI GCGC 1 cut(s) 362
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 4 cut(s) 392, 634, 813, 1160
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 4 cut(s) 82, 120, 123, 1211
LweI GCATC 7 cut(s) 184, 216, 339, 367, 595, 607, 790
MaeI CTAG 1 cut(s) 509
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 6 cut(s) 188, 253, 597, 774, 946, 1193
MalI GATC 4 cut(s) 394, 636, 815, 1162
MboI GATC 4 cut(s) 392, 634, 813, 1160
MflI RGATCY 3 cut(s) 634, 813, 1160
MhlI GDGCHC 1 cut(s) 986
MluCI AATT 6 cut(s) 31, 85, 370, 477, 987, 1032
MlyI GAGTC 3 cut(s) 1043, 1133, 1201
MmeI TCCRAC 2 cut(s) 285, 394
MnlI CCTC 7 cut(s) 268, 302, 466, 688, 717, 1129, 1264
Mph1103I ATGCAT 2 cut(s) 354, 382
MroXI GAANNNNTTC 1 cut(s) 371
MseI TTAA 3 cut(s) 66, 590, 1098
MslI CAYNNNNRTG 2 cut(s) 989, 1332
MspA1I CMGCKG 2 cut(s) 222, 1001
MspR9I CCNGG 2 cut(s) 322, 1187
MvaI CCWGG 2 cut(s) 322, 1187
MvnI CGCG 2 cut(s) 222, 837
NdeII GATC 4 cut(s) 392, 634, 813, 1160
NlaIII CATG 8 cut(s) 271, 347, 384, 658, 717, 740, 865, 1316
NmuCI GTSAC 4 cut(s) 188, 253, 946, 1193
NruI TCGCGA 1 cut(s) 837
NsiI ATGCAT 2 cut(s) 354, 382
NspI RCATGY 2 cut(s) 384, 658
PaeR7I CTCGAG 1 cut(s) 975
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 2 cut(s) 566, 1109
PkrI GCNGC 6 cut(s) 97, 110, 113, 224, 872, 1226
PleI GAGTC 3 cut(s) 1043, 1132, 1200
PpsI GAGTC 3 cut(s) 1043, 1132, 1200
Psp6I CCWGG 2 cut(s) 320, 1185
PspFI CCCAGC 1 cut(s) 45
PspGI CCWGG 2 cut(s) 320, 1185
PspPI GGNCC 2 cut(s) 311, 760
PstNI CAGNNNCTG 2 cut(s) 569, 752
PsuI RGATCY 3 cut(s) 634, 813, 1160
PvuII CAGCTG 1 cut(s) 1001
RruI TCGCGA 1 cut(s) 837
RsaI GTAC 4 cut(s) 342, 540, 789, 1274
RsaNI GTAC 4 cut(s) 341, 539, 788, 1273
RseI CAYNNNNRTG 2 cut(s) 989, 1332
SacII CCGCGG 1 cut(s) 223
SaqAI TTAA 3 cut(s) 66, 590, 1098
SatI GCNGC 6 cut(s) 96, 109, 112, 223, 871, 1225
Sau3AI GATC 4 cut(s) 392, 634, 813, 1160
Sau96I GGNCC 2 cut(s) 311, 760
SchI GAGTC 3 cut(s) 1043, 1133, 1201
ScrFI CCNGG 2 cut(s) 322, 1187
SduI GDGCHC 1 cut(s) 986
SfaNI GCATC 7 cut(s) 184, 216, 339, 367, 595, 607, 790
Sfr274I CTCGAG 1 cut(s) 975
Sfr303I CCGCGG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 223
SlaI CTCGAG 1 cut(s) 975
SmiMI CAYNNNNRTG 2 cut(s) 989, 1332
SmlI CTYRAG 1 cut(s) 975
SmoI CTYRAG 1 cut(s) 975
Sse9I AATT 6 cut(s) 31, 85, 370, 477, 987, 1032
SsiI CCGC 4 cut(s) 220, 222, 515, 871
SspI AATATT 1 cut(s) 103
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 2 cut(s) 320, 1185
StyI CCWWGG 1 cut(s) 763
TaaI ACNGT 6 cut(s) 131, 185, 754, 792, 892, 1296
TaiI ACGT 1 cut(s) 489
TaqI TCGA 5 cut(s) 210, 375, 722, 976, 1072
TasI AATT 6 cut(s) 31, 85, 370, 477, 987, 1032
TatI WGTACW 3 cut(s) 340, 787, 1272
TauI GCSGC 2 cut(s) 225, 873
TfiI GAWTC 2 cut(s) 566, 1109
Tru1I TTAA 3 cut(s) 66, 590, 1098
Tru9I TTAA 3 cut(s) 66, 590, 1098
TscAI CASTG 5 cut(s) 16, 136, 757, 922, 1339
TseFI GTSAC 4 cut(s) 188, 253, 946, 1193
TseI GCWGC 4 cut(s) 95, 108, 111, 1224
Tsp45I GTSAC 4 cut(s) 188, 253, 946, 1193
TspDTI ATGAA 5 cut(s) 78, 447, 477, 1010, 1122
TspRI CASTG 5 cut(s) 16, 136, 757, 922, 1339
VneI GTGCAC 1 cut(s) 982
XapI RAATTY 1 cut(s) 370
XceI RCATGY 2 cut(s) 384, 658
XhoI CTCGAG 1 cut(s) 975
XmiI GTMKAC 1 cut(s) 555
XmnI GAANNNNTTC 1 cut(s) 371
XspI CTAG 1 cut(s) 509
Zsp2I ATGCAT 2 cut(s) 354, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.