RLG00000023355

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013576
Physical Location & Seq
Reverse (-)
22434440 .. 22443747
9308 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023355

Sequence Viewer

Length: 2106 bp
ATGGTGCTTCCGGGTGTTTTGCCGGATCAGTTTACATTCCCGTTTGCATTGAGCGCGTGCTCAAAGATTGTGGCTTTCTGTGAAGGGGTTCAGCTCCATGGCTCGCTTGTGAAGATGGGTTTCGAGCAAGATGTGTTTATTGGGAATCCTTTGATTCATTTCTATGCAGAATGTGGGGACTTGGATTATGCCCACAAGGTGTCTGATGAAATGCTTGAGAAAAACATCGTGTCGTGGACTAGTTTGATGTGTGGTTATGGTAGAAGGAATATGCCCAAGGAGGCTGTTTCTTTGTTTTTGGAGATGGTGGCTGCCGGTGTTAAGCCCAATTCGGTGACTATGGTGTGTGTCATTTCTGCTTGTGCAAAGTTGAAGGATGTTGCGTTGAGTGAGAGAGTGTGTGCTTACATTGGGGAGTCTGGACTGAAGACTAATATGCTTATGGTGAATTCACTTGTTGATATGTATATGAAATGTGGAGCTACTGATGCCACAAAGCGGCTTTTTGATGAATGTGTCGATAAAAACTTGGTTCTTTACAATAACATTTTGTCAAATTATGTGCGCCAGGGACTGGCTAGTGAAGTAGTTTCTGTCATGGGTCAAATGCTACAACAGGGCCTAAGATCTGACAAGGTTACCATGTTAGCTGCAATTTCAGCTTGCGCACAACCAAGTGATTCTCTCTCAGATGCATTGCTTTCGGAAATGATAGACGAGTTGATTTACTTATTCCAACCACAAGAAGAAGAAGAGAACGAAGATGCCAAACACATCAATACCAATTCAATTGCTACCAACACAATCAGAAGCAACAACAGAAACGACAGCCCTGGCTTGTGCCTCAAGTGCAAGGCCAACCCTCCCATCTCCGCCGATACCCATGCCGACGATTCCCGTTTCTGCGCCGATTGCTTCCGCACAAACCTCTTCGGCAAGTTCAGGTTCGCCGTCACCTCCAACGCCATGATTTCTCCCACTGATAACGTCCTCGTCGCCTTCTCTGGCAGCCCTTCTTCCAGGGTTGCTTTACAGTTTGTACATGAGATGCACAGTAAAGCACAGAAGAATTTTGATGCTAGTAGGGATAGATCGTTACCGGTGTTCGGTGTTGGAGTTGCATTTATTGATGAAACTAGTGTTTATTCTGTTCCATCCAATGAACAAGACAATGCAATTGCAGACATTACATCTATTGTGGAAAGCCTAGCCCCGCCGACAAAACAATTGTACGTTGTACCGATTGAGAGTGTCTACTCTGAAGAGTCTGGCGATGGCAGGGAGAGACTAAAGAAGTTACTAGATGCTGTTACTGACGCCACTGGAAGAGAAGATCTTTTACTACATCTGCGGATGTTGGCCTTGCAAAAGATTGCCTCACAAAATGGATACAACAGACTTCTACTGGGATCATGCATATCGAGGATTGCTTGCCATGTTATTACAGCCACTGTGAAGGGACAAGGATATTCACTACCAGCAGATATACAGTATGTTGATTCAAGGTGGGAAATCCCGGTTTTGCTTCCTCTTCGTGATTGTCTTGCACAAGAGCTGGACATGCTTTGCCGCCTTGATGGTCTAAAAACTGTTGAGTTAATAAGAAGTCCTGGCTCTGGCATTAATGCGTTGGTTTCATCATTTGTGTCACTCTTGCAGGAAGAAAATCCTTCTCGGGAGTGCACAATTGTGAGAACAGCTGGAAAGCTTATCCCATTTCATTTCAACAGAATTCAAGAGATCGATGACTCTAATGTCCCTTTGGCAACTCGAAGGCATCGGAAGAGATATAACCTTAAACCTAATGAATGTTTTTCTTCAGAGTCATTCTGCCTTATCTGCAATGGCCCACTCAGCAAATCTGATTTGCTAAGTTTGCGCAATCTTGAACAATGCCAAACATGTCCCGACACTTTTGGTGGTGCCTGCTGTTCGAGCTGCCGGTTTCAGATACTTCCCCAGGACCCTTCATTACTGGATCAATTTTCTGCTATTTTACCCCAGCAGCTGGTTGCTCGGGCAAAGCATGACAACCTTGACAATTACAGTGTGCTCAGGGAACAAATACAAGATTGCTTGCTTTCAGAAGGCGAAGATGAAACCTGA

Protein Analysis

702

Amino Acids

77.36

Weight (kDa)

5.13

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 74 - 123 9.6e-13 PPR repeat family
PPR PF01535 77 - 107 2.4e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1754
Acc16I TGCGCA 2 cut(s) 667, 1880
AccB1I GGYRCC 1 cut(s) 1922
AccB7I CCANNNNNTGG 2 cut(s) 574, 2008
AccI GTMKAC 1 cut(s) 1254
AccII CGCG 1 cut(s) 56
AciI CCGC 6 cut(s) 499, 873, 919, 1214, 1351, 1570
AclWI GGATC 3 cut(s) 33, 1417, 1986
AcsI RAATTY 3 cut(s) 448, 1069, 1731
AcuI CTGAAG 3 cut(s) 446, 1281, 1803
AcyI GRCGYC 1 cut(s) 1317
AdeI CACNNNGTG 1 cut(s) 199
AfaI GTAC 3 cut(s) 1041, 1232, 1239
AfiI CCNNNNNNNGG 5 cut(s) 498, 574, 1106, 1616, 2008
AflIII ACRYGT 1 cut(s) 1901
AgeI ACCGGT 1 cut(s) 1099
AgsI TTSAA 6 cut(s) 373, 789, 1503, 1726, 1736, 1889
AhlI ACTAGT 2 cut(s) 239, 1136
AjnI CCWGG 5 cut(s) 567, 832, 1019, 1609, 1959
AleI CACNNNNGTG 1 cut(s) 1688
AluBI AGCT 9 cut(s) 94, 482, 650, 662, 1555, 1700, 1708, 1938, 2008
AluI AGCT 9 cut(s) 94, 482, 650, 662, 1555, 1700, 1708, 1938, 2008
Alw21I GWGCWC 3 cut(s) 62, 1685, 2055
Alw26I GTCTC 1 cut(s) 1279
Alw44I GTGCAC 1 cut(s) 1681
AlwI GGATC 3 cut(s) 33, 1417, 1986
AlwNI CAGNNNCTG 3 cut(s) 574, 1451, 2008
Ama87I CYCGRG 2 cut(s) 1674, 2016
AoxI GGCC 4 cut(s) 619, 855, 1359, 1846
ApaLI GTGCAC 1 cut(s) 1681
ApeKI GCWGC 5 cut(s) 311, 650, 1008, 1938, 2005
ApoI RAATTY 3 cut(s) 448, 1069, 1731
AseI ATTAAT 1 cut(s) 1623
AsiGI ACCGGT 1 cut(s) 1099
Asp700I GAANNNNTTC 1 cut(s) 87
AspLEI GCGC 5 cut(s) 56, 567, 668, 908, 1881
AspS9I GGNCC 3 cut(s) 619, 1847, 1963
AsuC2I CCSGG 2 cut(s) 12, 1517
AsuHPI GGTGA 3 cut(s) 346, 457, 946
AvaI CYCGRG 2 cut(s) 1674, 2016
AvaII GGWCC 1 cut(s) 1963
BaeGI GKGCMC 1 cut(s) 1685
BanI GGYRCC 1 cut(s) 1922
BarI GAAGNNNNNNTAC 2 cut(s) 1323, 1355
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 3 cut(s) 62, 1685, 2055
BbvI GCAGC 5 cut(s) 298, 637, 1020, 1925, 2017
BccI CCATC 6 cut(s) 109, 298, 875, 1162, 1268, 1571
BceAI ACGGC 1 cut(s) 935
BcgI CGANNNNNNTGC 4 cut(s) 684, 718, 866, 900
BciT130I CCWGG 5 cut(s) 569, 834, 1021, 1611, 1961
BciVI GTATCC 1 cut(s) 1382
BcnI CCSGG 2 cut(s) 12, 1517
BcoDI GTCTC 1 cut(s) 1279
BcuI ACTAGT 2 cut(s) 239, 1136
BfaI CTAG 6 cut(s) 240, 579, 1080, 1137, 1208, 1301
BfuI GTATCC 1 cut(s) 1382
BglII AGATCT 2 cut(s) 626, 1333
BisI GCNGC 7 cut(s) 312, 500, 651, 1009, 1570, 1939, 2006
BlsI GCNGC 7 cut(s) 313, 501, 652, 1010, 1571, 1940, 2007
Bme1390I CCNGG 7 cut(s) 12, 569, 834, 1021, 1517, 1611, 1961
Bme18I GGWCC 1 cut(s) 1963
BmeT110I CYCGRG 2 cut(s) 1674, 2016
BmgT120I GGNCC 3 cut(s) 619, 1847, 1963
BmiI GGNNCC 2 cut(s) 1924, 1965
BmrFI CCNGG 7 cut(s) 12, 569, 834, 1021, 1517, 1611, 1961
BmrI ACTGGG 1 cut(s) 1415
BmsI GCATC 7 cut(s) 478, 682, 754, 1038, 1066, 1294, 1786
BmuI ACTGGG 1 cut(s) 1415
BpiI GAAGAC 1 cut(s) 434
Bpu10I CCTNAGC 1 cut(s) 2054
BpuEI CTTGAG 2 cut(s) 236, 830
BpuMI CCSGG 2 cut(s) 12, 1517
Bsa29I ATCGAT 1 cut(s) 1743
BsaHI GRCGYC 1 cut(s) 1317
BsaJI CCNNGG 6 cut(s) 97, 276, 568, 832, 1020, 1959
BsaWI WCCGGW 1 cut(s) 1099
Bsc4I CCNNNNNNNGG 5 cut(s) 498, 574, 1106, 1616, 2008
Bse118I RCCGGY 3 cut(s) 314, 1099, 1941
Bse1I ACTGG 4 cut(s) 579, 1327, 1410, 1980
Bse3DI GCAATG 2 cut(s) 695, 1849
BseBI CCWGG 5 cut(s) 569, 834, 1021, 1611, 1961
BseCI ATCGAT 1 cut(s) 1743
BseDI CCNNGG 6 cut(s) 97, 276, 568, 832, 1020, 1959
BseGI GGATG 3 cut(s) 382, 1154, 1359
BseLI CCNNNNNNNGG 5 cut(s) 498, 574, 1106, 1616, 2008
BseMI GCAATG 2 cut(s) 695, 1849
BseMII CTCAG 3 cut(s) 702, 1867, 2068
BseNI ACTGG 4 cut(s) 579, 1327, 1410, 1980
BseSI GKGCMC 1 cut(s) 1685
BseXI GCAGC 5 cut(s) 298, 637, 1020, 1925, 2017
BseYI CCCAGC 1 cut(s) 2001
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 4 cut(s) 621, 857, 1361, 1848
BshNI GGYRCC 1 cut(s) 1922
BshTI ACCGGT 1 cut(s) 1099
BshVI ATCGAT 1 cut(s) 1743
BsiHKAI GWGCWC 3 cut(s) 62, 1685, 2055
BsiHKCI CYCGRG 2 cut(s) 1674, 2016
BsiSI CCGG 6 cut(s) 11, 23, 315, 1100, 1517, 1942
BslFI GGGAC 5 cut(s) 191, 585, 1473, 1742, 1890
BslI CCNNNNNNNGG 5 cut(s) 498, 574, 1106, 1616, 2008
BsmAI GTCTC 1 cut(s) 1279
BsmFI GGGAC 5 cut(s) 191, 585, 1473, 1742, 1890
BsnI GGCC 4 cut(s) 621, 857, 1361, 1848
BsoBI CYCGRG 2 cut(s) 1674, 2016
Bsp1286I GDGCHC 3 cut(s) 62, 1685, 2055
Bsp1407I TGTACA 1 cut(s) 1039
Bsp143I GATC 7 cut(s) 25, 626, 1091, 1333, 1409, 1740, 1978
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 6 cut(s) 499, 873, 919, 1214, 1351, 1570
BspANI GGCC 4 cut(s) 621, 857, 1361, 1848
BspCNI CTCAG 3 cut(s) 701, 1866, 2067
BspDI ATCGAT 1 cut(s) 1743
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 2 cut(s) 1924, 1965
BspPI GGATC 3 cut(s) 33, 1417, 1986
BspT107I GGYRCC 1 cut(s) 1922
BsrDI GCAATG 2 cut(s) 695, 1849
BsrFI RCCGGY 3 cut(s) 314, 1099, 1941
BsrGI TGTACA 1 cut(s) 1039
BsrI ACTGG 4 cut(s) 579, 1327, 1410, 1980
BssAI RCCGGY 3 cut(s) 314, 1099, 1941
BssECI CCNNGG 6 cut(s) 97, 276, 568, 832, 1020, 1959
BssMI GATC 7 cut(s) 25, 626, 1091, 1333, 1409, 1740, 1978
BssNI GRCGYC 1 cut(s) 1317
BssT1I CCWWGG 2 cut(s) 97, 276
Bst2UI CCWGG 5 cut(s) 569, 834, 1021, 1611, 1961
Bst4CI ACNGT 6 cut(s) 1035, 1055, 1453, 1491, 1591, 2048
Bst6I CTCTTC 6 cut(s) 747, 935, 1257, 1321, 1536, 1778
BstACI GRCGYC 1 cut(s) 1317
BstAUI TGTACA 1 cut(s) 1039
BstC8I GCNNGC 6 cut(s) 58, 104, 664, 1432, 1927, 2078
BstDEI CTNAG 5 cut(s) 623, 688, 1853, 1871, 2054
BstDSI CCRYGG 1 cut(s) 97
BstEII GGTNACC 1 cut(s) 637
BstF5I GGATG 3 cut(s) 382, 1154, 1359
BstFNI CGCG 1 cut(s) 56
BstHHI GCGC 5 cut(s) 56, 567, 668, 908, 1881
BstKTI GATC 7 cut(s) 28, 629, 1094, 1336, 1412, 1743, 1981
BstMAI GTCTC 1 cut(s) 1279
BstMBI GATC 7 cut(s) 25, 626, 1091, 1333, 1409, 1740, 1978
BstNI CCWGG 5 cut(s) 569, 834, 1021, 1611, 1961
BstNSI RCATGY 2 cut(s) 1564, 1905
BstPI GGTNACC 1 cut(s) 637
BstSCI CCNGG 7 cut(s) 10, 567, 832, 1019, 1515, 1609, 1959
BstSLI GKGCMC 1 cut(s) 1685
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 5 cut(s) 298, 637, 1020, 1925, 2017
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 2 cut(s) 626, 1333
BstYI RGATCY 2 cut(s) 626, 1333
Bsu15I ATCGAT 1 cut(s) 1743
BsuI GTATCC 1 cut(s) 1382
BsuRI GGCC 4 cut(s) 621, 857, 1361, 1848
BsuTUI ATCGAT 1 cut(s) 1743
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 1 cut(s) 1287
BtsCI GGATG 3 cut(s) 382, 1154, 1359
BtsIMutI CAGTG 4 cut(s) 978, 1320, 1449, 2053
Cac8I GCNNGC 6 cut(s) 58, 104, 664, 1432, 1927, 2078
CaiI CAGNNNCTG 3 cut(s) 574, 1451, 2008
CfoI GCGC 5 cut(s) 56, 567, 668, 908, 1881
Cfr10I RCCGGY 3 cut(s) 314, 1099, 1941
Cfr13I GGNCC 3 cut(s) 619, 1847, 1963
ClaI ATCGAT 1 cut(s) 1743
CseI GACGC 1 cut(s) 1325
Csp6I GTAC 3 cut(s) 1040, 1231, 1238
CspAI ACCGGT 1 cut(s) 1099
CspCI CAANNNNNGTGG 2 cut(s) 51, 86
CviQI GTAC 3 cut(s) 1040, 1231, 1238
DdeI CTNAG 5 cut(s) 623, 688, 1853, 1871, 2054
DpnI GATC 7 cut(s) 27, 628, 1093, 1335, 1411, 1742, 1980
DpnII GATC 7 cut(s) 25, 626, 1091, 1333, 1409, 1740, 1978
DraIII CACNNNGTG 1 cut(s) 199
DrdI GACNNNNNNGTC 1 cut(s) 1754
DseDI GACNNNNNNGTC 1 cut(s) 1754
Eam1104I CTCTTC 6 cut(s) 747, 935, 1257, 1321, 1536, 1778
EarI CTCTTC 6 cut(s) 747, 935, 1257, 1321, 1536, 1778
EciI GGCGGA 1 cut(s) 862
Eco130I CCWWGG 2 cut(s) 97, 276
Eco47I GGWCC 1 cut(s) 1963
Eco57I CTGAAG 3 cut(s) 446, 1281, 1803
Eco88I CYCGRG 2 cut(s) 1674, 2016
Eco91I GGTNACC 1 cut(s) 637
EcoO109I RGGNCCY 2 cut(s) 619, 1963
EcoO65I GGTNACC 1 cut(s) 637
EcoRI GAATTC 2 cut(s) 448, 1731
EcoRII CCWGG 5 cut(s) 567, 832, 1019, 1609, 1959
EcoT14I CCWWGG 2 cut(s) 97, 276
EcoT22I ATGCAT 2 cut(s) 697, 1418
ErhI CCWWGG 2 cut(s) 97, 276
FaqI GGGAC 5 cut(s) 191, 585, 1473, 1742, 1890
FauI CCCGC 1 cut(s) 1221
FblI GTMKAC 1 cut(s) 1254
Fnu4HI GCNGC 7 cut(s) 312, 500, 651, 1009, 1570, 1939, 2006
FokI GGATG 3 cut(s) 389, 1141, 1366
Fsp4HI GCNGC 7 cut(s) 312, 500, 651, 1009, 1570, 1939, 2006
FspBI CTAG 6 cut(s) 240, 579, 1080, 1137, 1208, 1301
FspI TGCGCA 2 cut(s) 667, 1880
GlaI GCGC 5 cut(s) 55, 566, 667, 907, 1880
GluI GCNGC 7 cut(s) 312, 500, 651, 1009, 1570, 1939, 2006
GsaI CCCAGC 1 cut(s) 2005
HaeIII GGCC 4 cut(s) 621, 857, 1361, 1848
HapII CCGG 6 cut(s) 11, 23, 315, 1100, 1517, 1942
HgaI GACGC 1 cut(s) 1325
HhaI GCGC 5 cut(s) 56, 567, 668, 908, 1881
Hin1I GRCGYC 1 cut(s) 1317
Hin6I GCGC 5 cut(s) 54, 565, 666, 906, 1879
HinP1I GCGC 5 cut(s) 54, 565, 666, 906, 1879
HindIII AAGCTT 1 cut(s) 1706
HinfI GANTC 9 cut(s) 145, 154, 416, 680, 893, 1265, 1499, 1748, 1823
HpaII CCGG 6 cut(s) 11, 23, 315, 1100, 1517, 1942
HphI GGTGA 3 cut(s) 346, 457, 946
Hpy166II GTNNAC 4 cut(s) 33, 237, 1255, 1683
Hpy188III TCNNGA 6 cut(s) 420, 1535, 1676, 1736, 1886, 1907
Hpy8I GTNNAC 4 cut(s) 33, 237, 1255, 1683
Hpy99I CGWCG 2 cut(s) 893, 998
HpyCH4III ACNGT 6 cut(s) 1035, 1055, 1453, 1491, 1591, 2048
HpyCH4IV ACGT 2 cut(s) 987, 1233
HpyF3I CTNAG 5 cut(s) 623, 688, 1853, 1871, 2054
HpySE526I ACGT 2 cut(s) 987, 1233
Hsp92I GRCGYC 1 cut(s) 1317
HspAI GCGC 5 cut(s) 54, 565, 666, 906, 1879
Kzo9I GATC 7 cut(s) 25, 626, 1091, 1333, 1409, 1740, 1978
LmnI GCTCC 2 cut(s) 99, 479
Lsp1109I GCAGC 5 cut(s) 298, 637, 1020, 1925, 2017
LweI GCATC 7 cut(s) 478, 682, 754, 1038, 1066, 1294, 1786
MaeI CTAG 6 cut(s) 240, 579, 1080, 1137, 1208, 1301
MaeII ACGT 2 cut(s) 987, 1233
MaeIII GTNAC 7 cut(s) 334, 637, 952, 1095, 1296, 1309, 1647
MalI GATC 7 cut(s) 27, 628, 1093, 1335, 1411, 1742, 1980
MboI GATC 7 cut(s) 25, 626, 1091, 1333, 1409, 1740, 1978
MfeI CAATTG 4 cut(s) 789, 1176, 1226, 1686
MflI RGATCY 2 cut(s) 626, 1333
MhlI GDGCHC 3 cut(s) 62, 1685, 2055
MlyI GAGTC 4 cut(s) 425, 1274, 1742, 1832
MmeI TCCRAC 3 cut(s) 760, 984, 1093
MnlI CCTC 9 cut(s) 274, 854, 873, 938, 967, 1001, 1387, 1416, 1539
Mph1103I ATGCAT 2 cut(s) 697, 1418
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 4 cut(s) 321, 1598, 1623, 1797
MslI CAYNNNNRTG 2 cut(s) 162, 1688
MspA1I CMGCKG 2 cut(s) 1700, 2008
MspI CCGG 6 cut(s) 11, 23, 315, 1100, 1517, 1942
MspR9I CCNGG 7 cut(s) 12, 569, 834, 1021, 1517, 1611, 1961
MunI CAATTG 4 cut(s) 789, 1176, 1226, 1686
MvaI CCWGG 5 cut(s) 569, 834, 1021, 1611, 1961
MvnI CGCG 1 cut(s) 56
NciI CCSGG 2 cut(s) 12, 1517
NcoI CCATGG 1 cut(s) 97
NdeII GATC 7 cut(s) 25, 626, 1091, 1333, 1409, 1740, 1978
NlaIV GGNNCC 2 cut(s) 1924, 1965
NmuCI GTSAC 3 cut(s) 334, 952, 1647
NsbI TGCGCA 2 cut(s) 667, 1880
NsiI ATGCAT 2 cut(s) 697, 1418
NspI RCATGY 2 cut(s) 1564, 1905
OliI CACNNNNGTG 1 cut(s) 1688
PciI ACATGT 1 cut(s) 1901
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 5 cut(s) 145, 154, 680, 893, 1499
PflMI CCANNNNNTGG 2 cut(s) 574, 2008
PinAI ACCGGT 1 cut(s) 1099
PkrI GCNGC 7 cut(s) 313, 501, 652, 1010, 1571, 1940, 2007
PleI GAGTC 4 cut(s) 424, 1273, 1742, 1831
PpsI GAGTC 4 cut(s) 424, 1273, 1742, 1831
PpuMI RGGWCCY 1 cut(s) 1963
PscI ACATGT 1 cut(s) 1901
PshBI ATTAAT 1 cut(s) 1623
Psp5II RGGWCCY 1 cut(s) 1963
Psp6I CCWGG 5 cut(s) 567, 832, 1019, 1609, 1959
PspEI GGTNACC 1 cut(s) 637
PspFI CCCAGC 1 cut(s) 2001
PspGI CCWGG 5 cut(s) 567, 832, 1019, 1609, 1959
PspN4I GGNNCC 2 cut(s) 1924, 1965
PspPI GGNCC 3 cut(s) 619, 1847, 1963
PspPPI RGGWCCY 1 cut(s) 1963
PstNI CAGNNNCTG 3 cut(s) 574, 1451, 2008
PsuI RGATCY 2 cut(s) 626, 1333
PvuII CAGCTG 2 cut(s) 1700, 2008
RsaI GTAC 3 cut(s) 1041, 1232, 1239
RsaNI GTAC 3 cut(s) 1040, 1231, 1238
RseI CAYNNNNRTG 2 cut(s) 162, 1688
SaqAI TTAA 4 cut(s) 321, 1598, 1623, 1797
SatI GCNGC 7 cut(s) 312, 500, 651, 1009, 1570, 1939, 2006
Sau3AI GATC 7 cut(s) 25, 626, 1091, 1333, 1409, 1740, 1978
Sau96I GGNCC 3 cut(s) 619, 1847, 1963
SchI GAGTC 4 cut(s) 425, 1274, 1742, 1832
ScrFI CCNGG 7 cut(s) 12, 569, 834, 1021, 1517, 1611, 1961
SduI GDGCHC 3 cut(s) 62, 1685, 2055
SfaNI GCATC 7 cut(s) 478, 682, 754, 1038, 1066, 1294, 1786
SinI GGWCC 1 cut(s) 1963
SmiMI CAYNNNNRTG 2 cut(s) 162, 1688
SmlI CTYRAG 2 cut(s) 215, 845
SmoI CTYRAG 2 cut(s) 215, 845
SpeI ACTAGT 2 cut(s) 239, 1136
SsiI CCGC 6 cut(s) 499, 873, 919, 1214, 1351, 1570
SspMI CTAG 6 cut(s) 240, 579, 1080, 1137, 1208, 1301
StyD4I CCNGG 7 cut(s) 10, 567, 832, 1019, 1515, 1609, 1959
StyI CCWWGG 2 cut(s) 97, 276
TaaI ACNGT 6 cut(s) 1035, 1055, 1453, 1491, 1591, 2048
TaiI ACGT 2 cut(s) 990, 1236
TaqI TCGA 6 cut(s) 123, 519, 1421, 1743, 1771, 1934
TatI WGTACW 1 cut(s) 1039
TauI GCSGC 2 cut(s) 502, 1572
TfiI GAWTC 5 cut(s) 145, 154, 680, 893, 1499
Tru1I TTAA 4 cut(s) 321, 1598, 1623, 1797
Tru9I TTAA 4 cut(s) 321, 1598, 1623, 1797
TscAI CASTG 4 cut(s) 985, 1327, 1456, 2053
TseFI GTSAC 3 cut(s) 334, 952, 1647
TseI GCWGC 5 cut(s) 311, 650, 1008, 1938, 2005
Tsp45I GTSAC 3 cut(s) 334, 952, 1647
TspRI CASTG 4 cut(s) 985, 1327, 1456, 2053
Van91I CCANNNNNTGG 2 cut(s) 574, 2008
VneI GTGCAC 1 cut(s) 1681
VpaK11BI GGWCC 1 cut(s) 1963
VspI ATTAAT 1 cut(s) 1623
XapI RAATTY 3 cut(s) 448, 1069, 1731
XceI RCATGY 2 cut(s) 1564, 1905
XmiI GTMKAC 1 cut(s) 1254
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 6 cut(s) 240, 579, 1080, 1137, 1208, 1301
Zsp2I ATGCAT 2 cut(s) 697, 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.