RLG00000023356

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013576
Physical Location & Seq
Reverse (-)
22465684 .. 22469401
3718 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023356

Sequence Viewer

Length: 1416 bp
ATGGCGTGCAGTGCCTCAACTTGCCAGTCAAATTGCTACAGAGACCAACCACAAGAAGAAGAAGAAGAAGATGCCAAACACACCAATACCAATTCAATTGCTACCAACACAATCAGCAACAACAACAACAGAAACAACAGCCATGGTTTGTGCCTCAAGTGCAAGGCCAACCCTCCCATCTCCGCCGATACCCACGCCGACGATTCCCGTTTCTGCGCCGATTGCTTCCGCGCAAACCTCTTCGGCAAGTTCAGGTTCGCCGTCACCTCCAACGCCATGATTTCTCCCACTGATAACGTCCTCGTCGCCTTCTCCGGCGGCCCTTCTTCCAGGGTTGCTTTACAGTTTGTACATGAGATGCACAGTAAAGCGCAGAAGAATTTTGATGCTAGTAGGGATAGATCGTTACCGGTGTTCGGTGTTGGAGTTGCATTTATTGATGAAACTAGTGTTTATTCTGTTCCATCCAATGAACAAGACAATGCAATTGAAGACATTACATCCATTGTGGAAAACCTAGCCCCGCCGACAAAACAATTGTACGTTGTACCGATTGAGAGTGTCTACTCTGAAGAGCCTGGCGATGCCAGGGAGAGACTAAAGAAGTTACTAGATGCTGTTACTGACGCCACTGGAAGAGAAGATCTTTTACTACATCTGCGGATGTTGGCCTTGCAAAAGATTGCCTCACAAAATGGATACAACAGACTTCTTCTGGGAGCATGCATATCGAGGATTGCTTGCCATGTTATTACAGCCACTGTGAAGGGACAAGGATATTCACTACCAGCAGATATACAGTATGTTGATTCAAGGTGGGAAATCCCAGTTTTGCTTCCTCTTCGTGATTGTCTTGCACAAGAGCTGAACATGCTTTGCCGCCTTGATGGTCTAAAAACTGTTGAGCTGATAAGAAGTCCTGGCTCTGGCATTAATGGGTTGGTTTCATCATTTGTGTCACTCTTGCAGGAAGAAAATCCTTCTCGGGAGTGCACAATTGTGAGAACAGCTGGAAAGCTTATCCCATTTCATTTCAACAGAATTCAAGAGATTGATGACTCTAATGTCCCTTTGGCAACTCAAAGGCGTCAGAAGAGATATAACCTTAAACCTAATGAATGTTTTTCTTCAGAGTCATTCTGCATTATCTGCAATGGCCCACTCAGCAAATCTGATTTGCTAAGTTTGCGCAATCTTGAACAATGCCAAACATGTCCCGACACTTTTGGTGGTGCCTGCTGTTCGAGCTGCCGGTTTCAGATACTTCCCCAGGACCCTTCATTACTGGATCAATTTTCTGCTGTTTTACCCCAGCAGCTGGTTGCTCGGGCAAAGCATGACAACCTTGACAATTACAGTGTGCTCAGGGAACAAATGCAAGATTGCTTGCTTTCAGAAGGCGAAGATGAAACCTGA

Protein Analysis

472

Amino Acids

52.14

Weight (kDa)

5.21

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1064
Acc16I TGCGCA 1 cut(s) 1190
AccB1I GGYRCC 1 cut(s) 1232
AccB7I CCANNNNNTGG 1 cut(s) 1318
AccI GTMKAC 1 cut(s) 564
AccII CGCG 1 cut(s) 231
AciI CCGC 6 cut(s) 183, 229, 318, 524, 661, 880
AclWI GGATC 1 cut(s) 1296
AcsI RAATTY 2 cut(s) 379, 1041
AcuI CTGAAG 2 cut(s) 591, 1113
AcyI GRCGYC 2 cut(s) 627, 1087
AfaI GTAC 3 cut(s) 351, 542, 549
AfiI CCNNNNNNNGG 3 cut(s) 416, 926, 1318
AflIII ACRYGT 1 cut(s) 1211
AgeI ACCGGT 1 cut(s) 409
AgsI TTSAA 6 cut(s) 96, 491, 813, 1036, 1046, 1199
AhlI ACTAGT 1 cut(s) 446
AjnI CCWGG 5 cut(s) 329, 577, 587, 919, 1269
AleI CACNNNNGTG 1 cut(s) 998
AluBI AGCT 6 cut(s) 865, 907, 1010, 1018, 1248, 1318
AluI AGCT 6 cut(s) 865, 907, 1010, 1018, 1248, 1318
Alw21I GWGCWC 2 cut(s) 995, 1365
Alw26I GTCTC 2 cut(s) 36, 589
Alw44I GTGCAC 1 cut(s) 991
AlwI GGATC 1 cut(s) 1296
AlwNI CAGNNNCTG 2 cut(s) 761, 1318
Ama87I CYCGRG 2 cut(s) 984, 1326
AoxI GGCC 4 cut(s) 165, 319, 669, 1156
ApaLI GTGCAC 1 cut(s) 991
ApeKI GCWGC 2 cut(s) 1248, 1315
ApoI RAATTY 2 cut(s) 379, 1041
AseI ATTAAT 1 cut(s) 933
AsiGI ACCGGT 1 cut(s) 409
AspLEI GCGC 4 cut(s) 218, 233, 373, 1191
AspS9I GGNCC 3 cut(s) 320, 1157, 1273
AsuHPI GGTGA 1 cut(s) 256
AvaI CYCGRG 2 cut(s) 984, 1326
AvaII GGWCC 1 cut(s) 1273
BaeGI GKGCMC 1 cut(s) 995
BanI GGYRCC 1 cut(s) 1232
BarI GAAGNNNNNNTAC 2 cut(s) 633, 665
BbsI GAAGAC 1 cut(s) 498
Bbv12I GWGCWC 2 cut(s) 995, 1365
BbvI GCAGC 2 cut(s) 1235, 1327
BccI CCATC 3 cut(s) 185, 472, 881
BceAI ACGGC 1 cut(s) 245
BcgI CGANNNNNNTGC 2 cut(s) 711, 745
BciT130I CCWGG 5 cut(s) 331, 579, 589, 921, 1271
BciVI GTATCC 1 cut(s) 692
BcoDI GTCTC 2 cut(s) 36, 589
BcuI ACTAGT 1 cut(s) 446
BfaI CTAG 4 cut(s) 390, 447, 518, 611
BfmI CTRYAG 1 cut(s) 37
BfuI GTATCC 1 cut(s) 692
BglII AGATCT 1 cut(s) 643
BisI GCNGC 4 cut(s) 319, 880, 1249, 1316
BlsI GCNGC 4 cut(s) 320, 881, 1250, 1317
Bme1390I CCNGG 5 cut(s) 331, 579, 589, 921, 1271
Bme18I GGWCC 1 cut(s) 1273
BmeT110I CYCGRG 2 cut(s) 984, 1326
BmgT120I GGNCC 3 cut(s) 320, 1157, 1273
BmiI GGNNCC 2 cut(s) 1234, 1275
BmrFI CCNGG 5 cut(s) 331, 579, 589, 921, 1271
BmrI ACTGGG 1 cut(s) 821
BmsI GCATC 5 cut(s) 61, 348, 376, 574, 604
BmuI ACTGGG 1 cut(s) 821
BpiI GAAGAC 1 cut(s) 498
Bpu10I CCTNAGC 1 cut(s) 1364
BpuEI CTTGAG 1 cut(s) 140
BsaHI GRCGYC 2 cut(s) 627, 1087
BsaI GGTCTC 1 cut(s) 36
BsaJI CCNNGG 4 cut(s) 142, 330, 588, 1269
BsaWI WCCGGW 1 cut(s) 409
Bsc4I CCNNNNNNNGG 3 cut(s) 416, 926, 1318
Bse118I RCCGGY 2 cut(s) 409, 1251
Bse1I ACTGG 4 cut(s) 25, 637, 827, 1290
Bse3DI GCAATG 1 cut(s) 1159
BseBI CCWGG 5 cut(s) 331, 579, 589, 921, 1271
BseDI CCNNGG 4 cut(s) 142, 330, 588, 1269
BseGI GGATG 3 cut(s) 464, 500, 669
BseLI CCNNNNNNNGG 3 cut(s) 416, 926, 1318
BseMI GCAATG 1 cut(s) 1159
BseMII CTCAG 2 cut(s) 1177, 1378
BseNI ACTGG 4 cut(s) 25, 637, 827, 1290
BseSI GKGCMC 1 cut(s) 995
BseXI GCAGC 2 cut(s) 1235, 1327
BseYI CCCAGC 1 cut(s) 1311
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 4 cut(s) 167, 321, 671, 1158
BshNI GGYRCC 1 cut(s) 1232
BshTI ACCGGT 1 cut(s) 409
BsiHKAI GWGCWC 2 cut(s) 995, 1365
BsiHKCI CYCGRG 2 cut(s) 984, 1326
BsiSI CCGG 3 cut(s) 315, 410, 1252
BslFI GGGAC 3 cut(s) 783, 1052, 1200
BslI CCNNNNNNNGG 3 cut(s) 416, 926, 1318
BsmAI GTCTC 2 cut(s) 36, 589
BsmFI GGGAC 3 cut(s) 783, 1052, 1200
BsnI GGCC 4 cut(s) 167, 321, 671, 1158
Bso31I GGTCTC 1 cut(s) 36
BsoBI CYCGRG 2 cut(s) 984, 1326
Bsp1286I GDGCHC 2 cut(s) 995, 1365
Bsp1407I TGTACA 1 cut(s) 349
Bsp143I GATC 3 cut(s) 401, 643, 1288
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 6 cut(s) 183, 229, 318, 524, 661, 880
BspANI GGCC 4 cut(s) 167, 321, 671, 1158
BspCNI CTCAG 2 cut(s) 1176, 1377
BspFNI CGCG 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 1234, 1275
BspPI GGATC 1 cut(s) 1296
BspQI GCTCTTC 1 cut(s) 567
BspT107I GGYRCC 1 cut(s) 1232
BspTNI GGTCTC 1 cut(s) 36
BsrDI GCAATG 1 cut(s) 1159
BsrFI RCCGGY 2 cut(s) 409, 1251
BsrGI TGTACA 1 cut(s) 349
BsrI ACTGG 4 cut(s) 25, 637, 827, 1290
BssAI RCCGGY 2 cut(s) 409, 1251
BssECI CCNNGG 4 cut(s) 142, 330, 588, 1269
BssMI GATC 3 cut(s) 401, 643, 1288
BssNI GRCGYC 2 cut(s) 627, 1087
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 5 cut(s) 331, 579, 589, 921, 1271
Bst4CI ACNGT 6 cut(s) 345, 365, 763, 801, 901, 1358
Bst6I CTCTTC 5 cut(s) 245, 567, 631, 846, 1088
BstACI GRCGYC 2 cut(s) 627, 1087
BstAPI GCANNNNNTGC 1 cut(s) 1149
BstAUI TGTACA 1 cut(s) 349
BstC8I GCNNGC 5 cut(s) 7, 724, 742, 1237, 1388
BstDEI CTNAG 3 cut(s) 1163, 1181, 1364
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 3 cut(s) 464, 500, 669
BstFNI CGCG 1 cut(s) 231
BstHHI GCGC 4 cut(s) 218, 233, 373, 1191
BstKTI GATC 3 cut(s) 404, 646, 1291
BstMAI GTCTC 2 cut(s) 36, 589
BstMBI GATC 3 cut(s) 401, 643, 1288
BstMWI GCNNNNNNNGC 8 cut(s) 11, 159, 222, 871, 1149, 1164, 1186, 1245
BstNI CCWGG 5 cut(s) 331, 579, 589, 921, 1271
BstNSI RCATGY 3 cut(s) 726, 874, 1215
BstSCI CCNGG 5 cut(s) 329, 577, 587, 919, 1269
BstSFI CTRYAG 1 cut(s) 37
BstSLI GKGCMC 1 cut(s) 995
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 1235, 1327
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 1 cut(s) 643
BstYI RGATCY 1 cut(s) 643
BsuI GTATCC 1 cut(s) 692
BsuRI GGCC 4 cut(s) 167, 321, 671, 1158
BtgI CCRYGG 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 597
BtsCI GGATG 3 cut(s) 464, 500, 669
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 5 cut(s) 16, 288, 630, 759, 1363
Cac8I GCNNGC 5 cut(s) 7, 724, 742, 1237, 1388
CaiI CAGNNNCTG 2 cut(s) 761, 1318
CfoI GCGC 4 cut(s) 218, 233, 373, 1191
Cfr10I RCCGGY 2 cut(s) 409, 1251
Cfr13I GGNCC 3 cut(s) 320, 1157, 1273
CseI GACGC 2 cut(s) 635, 1076
Csp6I GTAC 3 cut(s) 350, 541, 548
CspAI ACCGGT 1 cut(s) 409
CviAII CATG 8 cut(s) 143, 277, 353, 723, 746, 871, 1212, 1337
CviQI GTAC 3 cut(s) 350, 541, 548
DdeI CTNAG 3 cut(s) 1163, 1181, 1364
DpnI GATC 3 cut(s) 403, 645, 1290
DpnII GATC 3 cut(s) 401, 643, 1288
DrdI GACNNNNNNGTC 1 cut(s) 1064
DseDI GACNNNNNNGTC 1 cut(s) 1064
Eam1104I CTCTTC 5 cut(s) 245, 567, 631, 846, 1088
EarI CTCTTC 5 cut(s) 245, 567, 631, 846, 1088
EciI GGCGGA 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 1273
Eco57I CTGAAG 2 cut(s) 591, 1113
Eco88I CYCGRG 2 cut(s) 984, 1326
EcoO109I RGGNCCY 1 cut(s) 1273
EcoRI GAATTC 1 cut(s) 1041
EcoRII CCWGG 5 cut(s) 329, 577, 587, 919, 1269
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 728
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 8 cut(s) 146, 280, 356, 726, 749, 874, 1215, 1340
FaqI GGGAC 3 cut(s) 783, 1052, 1200
FatI CATG 8 cut(s) 142, 276, 352, 722, 745, 870, 1211, 1336
FauI CCCGC 1 cut(s) 531
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 4 cut(s) 319, 880, 1249, 1316
FokI GGATG 3 cut(s) 451, 487, 676
Fsp4HI GCNGC 4 cut(s) 319, 880, 1249, 1316
FspBI CTAG 4 cut(s) 390, 447, 518, 611
FspI TGCGCA 1 cut(s) 1190
GlaI GCGC 4 cut(s) 217, 232, 372, 1190
GluI GCNGC 4 cut(s) 319, 880, 1249, 1316
GsaI CCCAGC 1 cut(s) 1315
HaeIII GGCC 4 cut(s) 167, 321, 671, 1158
HapII CCGG 3 cut(s) 315, 410, 1252
HgaI GACGC 2 cut(s) 635, 1076
HhaI GCGC 4 cut(s) 218, 233, 373, 1191
Hin1I GRCGYC 2 cut(s) 627, 1087
Hin1II CATG 8 cut(s) 146, 280, 356, 726, 749, 874, 1215, 1340
Hin6I GCGC 4 cut(s) 216, 231, 371, 1189
HinP1I GCGC 4 cut(s) 216, 231, 371, 1189
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 4 cut(s) 203, 809, 1058, 1133
HpaII CCGG 3 cut(s) 315, 410, 1252
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 2 cut(s) 565, 993
Hpy188I TCNGA 6 cut(s) 571, 1092, 1132, 1174, 1260, 1396
Hpy188III TCNNGA 5 cut(s) 845, 986, 1046, 1196, 1217
Hpy8I GTNNAC 2 cut(s) 565, 993
Hpy99I CGWCG 2 cut(s) 203, 308
HpyAV CCTTC 6 cut(s) 319, 333, 760, 990, 1287, 1391
HpyCH4III ACNGT 6 cut(s) 345, 365, 763, 801, 901, 1358
HpyCH4IV ACGT 2 cut(s) 297, 543
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 159, 222, 871, 1149, 1164, 1186, 1245
HpyF3I CTNAG 3 cut(s) 1163, 1181, 1364
HpySE526I ACGT 2 cut(s) 297, 543
Hsp92I GRCGYC 2 cut(s) 627, 1087
Hsp92II CATG 8 cut(s) 146, 280, 356, 726, 749, 874, 1215, 1340
HspAI GCGC 4 cut(s) 216, 231, 371, 1189
Kzo9I GATC 3 cut(s) 401, 643, 1288
LguI GCTCTTC 1 cut(s) 567
LmnI GCTCC 1 cut(s) 719
Lsp1109I GCAGC 2 cut(s) 1235, 1327
LweI GCATC 5 cut(s) 61, 348, 376, 574, 604
MaeI CTAG 4 cut(s) 390, 447, 518, 611
MaeII ACGT 2 cut(s) 297, 543
MaeIII GTNAC 5 cut(s) 262, 405, 606, 619, 957
MalI GATC 3 cut(s) 403, 645, 1290
MboI GATC 3 cut(s) 401, 643, 1288
MfeI CAATTG 4 cut(s) 96, 486, 536, 996
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 2 cut(s) 995, 1365
MlyI GAGTC 2 cut(s) 1052, 1142
MmeI TCCRAC 2 cut(s) 294, 403
MnlI CCTC 9 cut(s) 25, 164, 183, 248, 277, 311, 697, 726, 849
Mph1103I ATGCAT 1 cut(s) 728
MseI TTAA 2 cut(s) 933, 1107
MslI CAYNNNNRTG 1 cut(s) 998
MspA1I CMGCKG 2 cut(s) 1010, 1318
MspI CCGG 3 cut(s) 315, 410, 1252
MspR9I CCNGG 5 cut(s) 331, 579, 589, 921, 1271
MunI CAATTG 4 cut(s) 96, 486, 536, 996
MvaI CCWGG 5 cut(s) 331, 579, 589, 921, 1271
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 8 cut(s) 11, 159, 222, 871, 1149, 1164, 1186, 1245
NcoI CCATGG 1 cut(s) 142
NdeII GATC 3 cut(s) 401, 643, 1288
NlaIII CATG 8 cut(s) 146, 280, 356, 726, 749, 874, 1215, 1340
NlaIV GGNNCC 2 cut(s) 1234, 1275
NmuCI GTSAC 2 cut(s) 262, 957
NsbI TGCGCA 1 cut(s) 1190
NsiI ATGCAT 1 cut(s) 728
NspI RCATGY 3 cut(s) 726, 874, 1215
OliI CACNNNNGTG 1 cut(s) 998
PaeI GCATGC 1 cut(s) 726
PciI ACATGT 1 cut(s) 1211
PciSI GCTCTTC 1 cut(s) 567
PfeI GAWTC 2 cut(s) 203, 809
PflMI CCANNNNNTGG 1 cut(s) 1318
PinAI ACCGGT 1 cut(s) 409
PkrI GCNGC 4 cut(s) 320, 881, 1250, 1317
PleI GAGTC 2 cut(s) 1052, 1141
PpsI GAGTC 2 cut(s) 1052, 1141
PpuMI RGGWCCY 1 cut(s) 1273
PscI ACATGT 1 cut(s) 1211
PshBI ATTAAT 1 cut(s) 933
Psp5II RGGWCCY 1 cut(s) 1273
Psp6I CCWGG 5 cut(s) 329, 577, 587, 919, 1269
PspFI CCCAGC 1 cut(s) 1311
PspGI CCWGG 5 cut(s) 329, 577, 587, 919, 1269
PspN4I GGNNCC 2 cut(s) 1234, 1275
PspPI GGNCC 3 cut(s) 320, 1157, 1273
PspPPI RGGWCCY 1 cut(s) 1273
PstNI CAGNNNCTG 2 cut(s) 761, 1318
PsuI RGATCY 1 cut(s) 643
PvuII CAGCTG 2 cut(s) 1010, 1318
RsaI GTAC 3 cut(s) 351, 542, 549
RsaNI GTAC 3 cut(s) 350, 541, 548
RseI CAYNNNNRTG 1 cut(s) 998
SapI GCTCTTC 1 cut(s) 567
SaqAI TTAA 2 cut(s) 933, 1107
SatI GCNGC 4 cut(s) 319, 880, 1249, 1316
Sau3AI GATC 3 cut(s) 401, 643, 1288
Sau96I GGNCC 3 cut(s) 320, 1157, 1273
SchI GAGTC 2 cut(s) 1052, 1142
ScrFI CCNGG 5 cut(s) 331, 579, 589, 921, 1271
SduI GDGCHC 2 cut(s) 995, 1365
SfaNI GCATC 5 cut(s) 61, 348, 376, 574, 604
SfcI CTRYAG 1 cut(s) 37
SinI GGWCC 1 cut(s) 1273
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
SpeI ACTAGT 1 cut(s) 446
SphI GCATGC 1 cut(s) 726
SsiI CCGC 6 cut(s) 183, 229, 318, 524, 661, 880
SspMI CTAG 4 cut(s) 390, 447, 518, 611
StyD4I CCNGG 5 cut(s) 329, 577, 587, 919, 1269
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 6 cut(s) 345, 365, 763, 801, 901, 1358
TaiI ACGT 2 cut(s) 300, 546
TaqI TCGA 2 cut(s) 731, 1244
TatI WGTACW 1 cut(s) 349
TauI GCSGC 2 cut(s) 321, 882
TfiI GAWTC 2 cut(s) 203, 809
Tru1I TTAA 2 cut(s) 933, 1107
Tru9I TTAA 2 cut(s) 933, 1107
TscAI CASTG 5 cut(s) 16, 295, 637, 766, 1363
TseFI GTSAC 2 cut(s) 262, 957
TseI GCWGC 2 cut(s) 1248, 1315
Tsp45I GTSAC 2 cut(s) 262, 957
TspDTI ATGAA 6 cut(s) 456, 486, 936, 1019, 1131, 1269
TspRI CASTG 5 cut(s) 16, 295, 637, 766, 1363
Van91I CCANNNNNTGG 1 cut(s) 1318
VneI GTGCAC 1 cut(s) 991
VpaK11BI GGWCC 1 cut(s) 1273
VspI ATTAAT 1 cut(s) 933
XapI RAATTY 2 cut(s) 379, 1041
XceI RCATGY 3 cut(s) 726, 874, 1215
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 4 cut(s) 390, 447, 518, 611
Zsp2I ATGCAT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.