MD12G1074000.v1.1

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013576
Physical Location & Seq
Forward (+)
8841317 .. 8845148
3832 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1074000.v1.1.491

Sequence Viewer

Length: 1398 bp
ATGGCGTGCAGTGCTTCAGGTTGCGAATCAAATTGCTACAAAACCCAACAAGAAGAAGATGAAGATGTCAAAGACAACAACAATTACAAATCAATTTCTACAAACACTAGAAACAACAACAGCAACAGTAGTTTGTGCCTCAAGTGCAAGGCCAATCAACCCATCTCTTCCAACACCGACGGCGGCGACGAAGCGCGCTTCTGCGCCGATTGCTTCCGCGGCAATCTCTTCGGGAAGTTCAGGTTCGCTGTCACCTCCAACGCCATGATTTCTCCTACTGACAATGTCCTCGTTGCCTTCTCCGGTGGCCCTTCTTCCAGGGTTGCTTTGCAGTTTGTACATGAGATGCATAATAAAGCACAGAAGAATTTCGATGCCAGTAGGGATAGATCGTTGCCAGTTTTTGGGGTTGGAGTTGCATTTGTTGATGAGACTTCTGTTTATTCTGTTCCGTCTGGTGAAGTAGAAAACGCAATTGAAGATATTAGATTGATCGTGGAAGACCTAGCTCCACCAACAAAAGAACTGCATGTTGTACCAATTGAAAGTGTATACTCTTCAGATTCTGGTGATGGAAGGGATAGATTAAATCAGTTGTTAGATGCTGTTCCTGATGCTACTGGGAAAGAAGATCTTTTACTTCACATGCGGATGTTGGCATTGCAAAAGGTTGCCTCTGAAAAGGGATACAACAGACTTGTGCTTGGATCATGCATATCGAGGATTGCTTGCCATGTTATTACAGCCACTGTCAAGGGCCAAGGATATTCTTTACCAGCTGATATACAATATGTTGATGCAAGGTGGGAGATCCCAGTGTTACTTCCTCTTCGTGATTGTCTTGCACGAGAGCTGACCATGCTTTGCCGCCTTGATGGGTTTGGCAGTCTAAAAACTGTGGAGTTGATGAGAAGTCCTAGTACTGGAATAAACGGCTTGGTCTCATCATTCGTGACACTTTTGCAGGAAGAAAATCCTTCTCGAGAGTGCACAATTGTGAGAACTGCTGGAAAGCTTATCCCATTTCATTTTAACGGAATTCAAGAGATAAATGACTCTAAGGTTTCTTTGGAAACTCGAAGGCGTCTGAAGAGATATAACCTTAAACCTAATGAATCTTTTTCCTCAGAGTCATTCTGCTTTATCTGCAATAGCCCACTCAGCCGATCTGACTTGCTAAGTTTGAAGAATTTTGAGGAGCACAAGACAAGTTATGTCTGCTGTTTAAGCTGCCAGTTTCAGATACTTCCTGAAGACCCCTCATCAATAAATCAATTTTTTACTCTTTTTCCTCAGCAGCTGGCTGCTCGAGCAAAGCATGACAATCTTGACCATTACAGTGTCCTCAGGGAACAAATACAAGATTGCTTGCTCTCAGAAGGTGAAGATGAGACTTGA

Protein Analysis

466

Amino Acids

51.66

Weight (kDa)

5.3

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 404
AccI GTMKAC 1 cut(s) 552
AccII CGCG 2 cut(s) 196, 219
AciI CCGC 5 cut(s) 183, 217, 219, 649, 868
AclWI GGATC 2 cut(s) 715, 805
AcsI RAATTY 3 cut(s) 367, 1038, 1189
AcuI CTGAAG 3 cut(s) 543, 1109, 1272
AcyI GRCGYC 1 cut(s) 1084
AfaI GTAC 3 cut(s) 339, 537, 922
AfiI CCNNNNNNNGG 2 cut(s) 404, 923
AgsI TTSAA 4 cut(s) 479, 545, 1043, 1186
AjnI CCWGG 1 cut(s) 317
AleI CACNNNNGTG 1 cut(s) 995
AluBI AGCT 6 cut(s) 509, 779, 853, 1015, 1230, 1300
AluI AGCT 6 cut(s) 509, 779, 853, 1015, 1230, 1300
Alw21I GWGCWC 2 cut(s) 992, 1203
Alw26I GTCTC 3 cut(s) 425, 946, 1385
Alw44I GTGCAC 1 cut(s) 988
AlwI GGATC 2 cut(s) 715, 805
AlwNI CAGNNNCTG 3 cut(s) 566, 749, 1300
Ama87I CYCGRG 2 cut(s) 981, 1308
AoxI GGCC 3 cut(s) 150, 307, 757
ApaLI GTGCAC 1 cut(s) 988
ApeKI GCWGC 3 cut(s) 1230, 1297, 1304
ApoI RAATTY 3 cut(s) 367, 1038, 1189
Asp700I GAANNNNTTC 1 cut(s) 368
AspLEI GCGC 3 cut(s) 196, 198, 206
AspS9I GGNCC 2 cut(s) 308, 757
AsuHPI GGTGA 4 cut(s) 244, 470, 581, 1394
AvaI CYCGRG 2 cut(s) 981, 1308
AxyI CCTNAGG 1 cut(s) 1346
BaeGI GKGCMC 1 cut(s) 992
BarI GAAGNNNNNNTAC 4 cut(s) 621, 653, 904, 936
BauI CACGAG 1 cut(s) 846
BbsI GAAGAC 2 cut(s) 507, 1260
Bbv12I GWGCWC 2 cut(s) 992, 1203
BbvCI CCTCAGC 1 cut(s) 1293
BbvI GCAGC 3 cut(s) 1217, 1291, 1309
BccI CCATC 3 cut(s) 170, 566, 869
BceAI ACGGC 2 cut(s) 196, 949
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 1 cut(s) 319
BciVI GTATCC 1 cut(s) 680
BcoDI GTCTC 3 cut(s) 425, 946, 1385
BfaI CTAG 3 cut(s) 108, 506, 918
BfuI GTATCC 1 cut(s) 680
BglII AGATCT 1 cut(s) 631
BisI GCNGC 6 cut(s) 184, 220, 868, 1231, 1298, 1305
BlsI GCNGC 6 cut(s) 185, 221, 869, 1232, 1299, 1306
BmcAI AGTACT 1 cut(s) 922
Bme1390I CCNGG 1 cut(s) 319
BmeT110I CYCGRG 2 cut(s) 981, 1308
BmgT120I GGNCC 2 cut(s) 308, 757
BmrFI CCNGG 1 cut(s) 319
BmrI ACTGGG 2 cut(s) 630, 809
BmsI GCATC 5 cut(s) 336, 364, 592, 604, 787
BmuI ACTGGG 2 cut(s) 630, 809
BpiI GAAGAC 2 cut(s) 507, 1260
Bpu10I CCTNAGC 1 cut(s) 1293
BpuEI CTTGAG 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 1084
BsaI GGTCTC 1 cut(s) 946
BsaJI CCNNGG 3 cut(s) 217, 318, 760
BsaWI WCCGGW 1 cut(s) 302
Bsc4I CCNNNNNNNGG 2 cut(s) 404, 923
Bse1I ACTGG 6 cut(s) 378, 398, 625, 815, 928, 1234
Bse21I CCTNAGG 1 cut(s) 1346
Bse3DI GCAATG 1 cut(s) 659
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 3 cut(s) 217, 318, 760
BseGI GGATG 1 cut(s) 657
BseLI CCNNNNNNNGG 2 cut(s) 404, 923
BseMI GCAATG 1 cut(s) 659
BseMII CTCAG 5 cut(s) 1140, 1174, 1307, 1360, 1389
BseNI ACTGG 6 cut(s) 378, 398, 625, 815, 928, 1234
BsePI GCGCGC 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 1211
BseSI GKGCMC 1 cut(s) 992
BseXI GCAGC 3 cut(s) 1217, 1291, 1309
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 2 cut(s) 196, 219
BshFI GGCC 3 cut(s) 152, 309, 759
BsiHKAI GWGCWC 2 cut(s) 992, 1203
BsiHKCI CYCGRG 2 cut(s) 981, 1308
BsiSI CCGG 1 cut(s) 303
BslI CCNNNNNNNGG 2 cut(s) 404, 923
BsmAI GTCTC 3 cut(s) 425, 946, 1385
BsnI GGCC 3 cut(s) 152, 309, 759
Bso31I GGTCTC 1 cut(s) 946
BsoBI CYCGRG 2 cut(s) 981, 1308
Bsp1286I GDGCHC 2 cut(s) 992, 1203
Bsp1407I TGTACA 1 cut(s) 337
Bsp143I GATC 6 cut(s) 389, 492, 631, 707, 810, 1166
BspACI CCGC 5 cut(s) 183, 217, 219, 649, 868
BspANI GGCC 3 cut(s) 152, 309, 759
BspCNI CTCAG 5 cut(s) 1139, 1173, 1306, 1359, 1388
BspFNI CGCG 2 cut(s) 196, 219
BspPI GGATC 2 cut(s) 715, 805
BspTNI GGTCTC 1 cut(s) 946
BsrDI GCAATG 1 cut(s) 659
BsrGI TGTACA 1 cut(s) 337
BsrI ACTGG 6 cut(s) 378, 398, 625, 815, 928, 1234
BssECI CCNNGG 3 cut(s) 217, 318, 760
BssHII GCGCGC 1 cut(s) 194
BssMI GATC 6 cut(s) 389, 492, 631, 707, 810, 1166
BssNAI GTATAC 1 cut(s) 553
BssNI GRCGYC 1 cut(s) 1084
BssSI CACGAG 1 cut(s) 846
BssT1I CCWWGG 1 cut(s) 760
Bst1107I GTATAC 1 cut(s) 553
Bst2BI CACGAG 1 cut(s) 846
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 4 cut(s) 128, 751, 898, 1340
Bst6I CTCTTC 5 cut(s) 172, 233, 562, 834, 1085
BstACI GRCGYC 1 cut(s) 1084
BstAUI TGTACA 1 cut(s) 337
BstC8I GCNNGC 5 cut(s) 7, 196, 730, 1302, 1370
BstDEI CTNAG 7 cut(s) 1059, 1126, 1160, 1178, 1293, 1346, 1375
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 1 cut(s) 657
BstFNI CGCG 2 cut(s) 196, 219
BstHHI GCGC 3 cut(s) 196, 198, 206
BstKTI GATC 6 cut(s) 392, 495, 634, 710, 813, 1169
BstMAI GTCTC 3 cut(s) 425, 946, 1385
BstMBI GATC 6 cut(s) 389, 492, 631, 707, 810, 1166
BstMWI GCNNNNNNNGC 9 cut(s) 11, 144, 210, 219, 859, 1146, 1161, 1227, 1310
BstNI CCWGG 1 cut(s) 319
BstNSI RCATGY 2 cut(s) 533, 649
BstSCI CCNGG 1 cut(s) 317
BstSLI GKGCMC 1 cut(s) 992
BstUI CGCG 2 cut(s) 196, 219
BstV1I GCAGC 3 cut(s) 1217, 1291, 1309
BstV2I GAAGAC 2 cut(s) 507, 1260
BstX2I RGATCY 2 cut(s) 631, 810
BstYI RGATCY 2 cut(s) 631, 810
BstZ17I GTATAC 1 cut(s) 553
Bsu36I CCTNAGG 1 cut(s) 1346
BsuI GTATCC 1 cut(s) 680
BsuRI GGCC 3 cut(s) 152, 309, 759
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 1 cut(s) 657
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 4 cut(s) 16, 747, 822, 1345
Cac8I GCNNGC 5 cut(s) 7, 196, 730, 1302, 1370
CaiI CAGNNNCTG 3 cut(s) 566, 749, 1300
CfoI GCGC 3 cut(s) 196, 198, 206
Cfr13I GGNCC 2 cut(s) 308, 757
Cfr42I CCGCGG 1 cut(s) 220
CseI GACGC 1 cut(s) 1073
Csp6I GTAC 3 cut(s) 338, 536, 921
CviAII CATG 8 cut(s) 265, 341, 530, 646, 711, 734, 859, 1319
CviQI GTAC 3 cut(s) 338, 536, 921
DdeI CTNAG 7 cut(s) 1059, 1126, 1160, 1178, 1293, 1346, 1375
DpnI GATC 6 cut(s) 391, 494, 633, 709, 812, 1168
DpnII GATC 6 cut(s) 389, 492, 631, 707, 810, 1166
Eam1104I CTCTTC 5 cut(s) 172, 233, 562, 834, 1085
EarI CTCTTC 5 cut(s) 172, 233, 562, 834, 1085
Eco130I CCWWGG 1 cut(s) 760
Eco31I GGTCTC 1 cut(s) 946
Eco57I CTGAAG 3 cut(s) 543, 1109, 1272
Eco81I CCTNAGG 1 cut(s) 1346
Eco88I CYCGRG 2 cut(s) 981, 1308
EcoRI GAATTC 1 cut(s) 1038
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 760
EcoT22I ATGCAT 2 cut(s) 351, 716
ErhI CCWWGG 1 cut(s) 760
FaeI CATG 8 cut(s) 268, 344, 533, 649, 714, 737, 862, 1322
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FatI CATG 8 cut(s) 264, 340, 529, 645, 710, 733, 858, 1318
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 6 cut(s) 184, 220, 868, 1231, 1298, 1305
FokI GGATG 1 cut(s) 664
Fsp4HI GCNGC 6 cut(s) 184, 220, 868, 1231, 1298, 1305
FspBI CTAG 3 cut(s) 108, 506, 918
GlaI GCGC 3 cut(s) 195, 197, 205
GluI GCNGC 6 cut(s) 184, 220, 868, 1231, 1298, 1305
HaeIII GGCC 3 cut(s) 152, 309, 759
HapII CCGG 1 cut(s) 303
HgaI GACGC 1 cut(s) 1073
HhaI GCGC 3 cut(s) 196, 198, 206
Hin1I GRCGYC 1 cut(s) 1084
Hin1II CATG 8 cut(s) 268, 344, 533, 649, 714, 737, 862, 1322
Hin6I GCGC 3 cut(s) 194, 196, 204
HinP1I GCGC 3 cut(s) 194, 196, 204
HindIII AAGCTT 1 cut(s) 1013
HinfI GANTC 5 cut(s) 26, 563, 1055, 1115, 1130
HpaII CCGG 1 cut(s) 303
HphI GGTGA 4 cut(s) 244, 470, 581, 1394
Hpy166II GTNNAC 2 cut(s) 553, 990
Hpy188I TCNGA 7 cut(s) 562, 679, 1089, 1129, 1171, 1242, 1378
Hpy188III TCNNGA 9 cut(s) 232, 611, 833, 952, 981, 983, 1043, 1250, 1328
Hpy8I GTNNAC 2 cut(s) 553, 990
Hpy99I CGWCG 2 cut(s) 182, 191
HpyAV CCTTC 6 cut(s) 307, 321, 570, 987, 1074, 1373
HpyCH4III ACNGT 4 cut(s) 128, 751, 898, 1340
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 144, 210, 219, 859, 1146, 1161, 1227, 1310
HpyF3I CTNAG 7 cut(s) 1059, 1126, 1160, 1178, 1293, 1346, 1375
Hsp92I GRCGYC 1 cut(s) 1084
Hsp92II CATG 8 cut(s) 268, 344, 533, 649, 714, 737, 862, 1322
HspAI GCGC 3 cut(s) 194, 196, 204
KspI CCGCGG 1 cut(s) 220
Kzo9I GATC 6 cut(s) 389, 492, 631, 707, 810, 1166
LmnI GCTCC 2 cut(s) 514, 1198
Lsp1109I GCAGC 3 cut(s) 1217, 1291, 1309
LweI GCATC 5 cut(s) 336, 364, 592, 604, 787
MaeI CTAG 3 cut(s) 108, 506, 918
MaeIII GTNAC 3 cut(s) 250, 819, 952
MalI GATC 6 cut(s) 391, 494, 633, 709, 812, 1168
MboI GATC 6 cut(s) 389, 492, 631, 707, 810, 1166
MfeI CAATTG 3 cut(s) 474, 540, 993
MflI RGATCY 2 cut(s) 631, 810
MhlI GDGCHC 2 cut(s) 992, 1203
MlyI GAGTC 2 cut(s) 1049, 1139
MmeI TCCRAC 3 cut(s) 195, 282, 391
Mph1103I ATGCAT 2 cut(s) 351, 716
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 4 cut(s) 587, 1032, 1104, 1226
MslI CAYNNNNRTG 3 cut(s) 650, 995, 1338
MspA1I CMGCKG 3 cut(s) 219, 779, 1300
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 1 cut(s) 319
MunI CAATTG 3 cut(s) 474, 540, 993
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 2 cut(s) 196, 219
MwoI GCNNNNNNNGC 9 cut(s) 11, 144, 210, 219, 859, 1146, 1161, 1227, 1310
NdeII GATC 6 cut(s) 389, 492, 631, 707, 810, 1166
NlaIII CATG 8 cut(s) 268, 344, 533, 649, 714, 737, 862, 1322
NmuCI GTSAC 2 cut(s) 250, 952
NsiI ATGCAT 2 cut(s) 351, 716
NspI RCATGY 2 cut(s) 533, 649
OliI CACNNNNGTG 1 cut(s) 995
PaeR7I CTCGAG 2 cut(s) 981, 1308
PauI GCGCGC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 3 cut(s) 26, 563, 1115
PflFI GACNNNGTC 1 cut(s) 284
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 6 cut(s) 185, 221, 869, 1232, 1299, 1306
PleI GAGTC 2 cut(s) 1049, 1138
PpsI GAGTC 2 cut(s) 1049, 1138
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspPI GGNCC 2 cut(s) 308, 757
PspXI VCTCGAGB 1 cut(s) 1308
PstNI CAGNNNCTG 3 cut(s) 566, 749, 1300
PsuI RGATCY 2 cut(s) 631, 810
PsyI GACNNNGTC 1 cut(s) 284
PteI GCGCGC 1 cut(s) 194
PvuII CAGCTG 2 cut(s) 779, 1300
RsaI GTAC 3 cut(s) 339, 537, 922
RsaNI GTAC 3 cut(s) 338, 536, 921
RseI CAYNNNNRTG 3 cut(s) 650, 995, 1338
SacII CCGCGG 1 cut(s) 220
SaqAI TTAA 4 cut(s) 587, 1032, 1104, 1226
SatI GCNGC 6 cut(s) 184, 220, 868, 1231, 1298, 1305
Sau3AI GATC 6 cut(s) 389, 492, 631, 707, 810, 1166
Sau96I GGNCC 2 cut(s) 308, 757
ScaI AGTACT 1 cut(s) 922
SchI GAGTC 2 cut(s) 1049, 1139
ScrFI CCNGG 1 cut(s) 319
SduI GDGCHC 2 cut(s) 992, 1203
SfaNI GCATC 5 cut(s) 336, 364, 592, 604, 787
Sfr274I CTCGAG 2 cut(s) 981, 1308
Sfr303I CCGCGG 1 cut(s) 220
SgrBI CCGCGG 1 cut(s) 220
SlaI CTCGAG 2 cut(s) 981, 1308
SmiMI CAYNNNNRTG 3 cut(s) 650, 995, 1338
SmlI CTYRAG 3 cut(s) 140, 981, 1308
SmoI CTYRAG 3 cut(s) 140, 981, 1308
SsiI CCGC 5 cut(s) 183, 217, 219, 649, 868
SspMI CTAG 3 cut(s) 108, 506, 918
StyD4I CCNGG 1 cut(s) 317
StyI CCWWGG 1 cut(s) 760
TaaI ACNGT 4 cut(s) 128, 751, 898, 1340
TaqI TCGA 5 cut(s) 372, 719, 982, 1078, 1309
TatI WGTACW 2 cut(s) 337, 920
TauI GCSGC 3 cut(s) 186, 222, 870
TfiI GAWTC 3 cut(s) 26, 563, 1115
Tru1I TTAA 4 cut(s) 587, 1032, 1104, 1226
Tru9I TTAA 4 cut(s) 587, 1032, 1104, 1226
TscAI CASTG 4 cut(s) 16, 754, 822, 1345
TseFI GTSAC 2 cut(s) 250, 952
TseI GCWGC 3 cut(s) 1230, 1297, 1304
Tsp45I GTSAC 2 cut(s) 250, 952
TspDTI ATGAA 3 cut(s) 75, 1016, 1128
TspGWI ACGGA 2 cut(s) 441, 1050
TspRI CASTG 4 cut(s) 16, 754, 822, 1345
Tth111I GACNNNGTC 1 cut(s) 284
Van91I CCANNNNNTGG 1 cut(s) 404
VneI GTGCAC 1 cut(s) 988
XapI RAATTY 3 cut(s) 367, 1038, 1189
XceI RCATGY 2 cut(s) 533, 649
XhoI CTCGAG 2 cut(s) 981, 1308
XmiI GTMKAC 1 cut(s) 552
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 3 cut(s) 108, 506, 918
ZrmI AGTACT 1 cut(s) 922
Zsp2I ATGCAT 2 cut(s) 351, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.